JP2019030232A - Generation method of shiitake mushroom - Google Patents

Abstract

To provide a generation method in which yield and weight for one mushroom are increased, by applying a novel germination processing in a generation step of shiitake mushroom.SOLUTION: The generation method of shiitake mushroom is configured so that, to a coating sheet formed on a surface of a mushroom bed in a step for inoculating shiitake mushroom and decomposing and decaying a culture medium by mycelium infestation for culturing the shiitake mushroom, germination processing for forming pores having a cross section of 0.5-20 mmreaching a mycelium layer under a surface coating sheet by penetrating a surface coating sheet is executed. A depth of the pore is 10-20 mm, and an interval between pores is 2-6 pieces/10mm.SELECTED DRAWING: Figure 1

Description

The present invention relates to a method for generating shiitake mushrooms in artificial cultivation of shiitake mushrooms, in particular fungal bed cultivation.

Conventionally, in the cultivation of shiitake mushrooms using a fungus bed, it has been necessary to give a germination treatment each time when shiitake mushrooms are generated several times in one fungus bed.
In general methods of this germination treatment, as described in cited references 1 to 5,
1) Method of changing the fungus bed management temperature in the range of about 5-10 ° C. (temperature change method) 2) Method of immersing the fungus bed in water (immersion method) 3) Tap the fungus bed with hand or special equipment There are known methods (implantation method), 4) a method of injecting water into the inside of the fungus bed (water injection method), 5) a method of changing the position of the shelf up and down, etc. (moving bed method).
In addition to this, in top cultivation where mushrooms are generated from the upper surface of the fungus bed, 6) a method of draining and re-supplying water accumulated between the fungus bed and the cultivation bag (side water drainage method) 7) A method of inverting the fungus bed and immersing only the upper surface portion in a fruit pack filled with water (partial water immersion method) is known.

Each of these germination methods is characterized by the magnitude of the effect and the range of application. In actual cultivation, the cultivation conditions, the purpose of cultivation, the varieties used, etc. are properly used according to the characteristics. However, each of them has advantages and disadvantages, and there are problems such as time and labor required and difficulty in adjusting germination.
For example, in the above-mentioned 2) flooding, (6) the reverse drainage method in top cultivation, and (7) the partial flooding method using fruit packs, it is essential to transport and maintain individual fungal beds. There is a problem that it takes a lot of labor.
On the other hand, although the implantation method of (3) requires relatively little labor, there is a problem in that it is difficult to adjust the germination because variation tends to occur among individual workers performing the treatment.

Therefore, when the present inventor comprehensively examined the problems (1) to (7) above, (a) they lack scientific evidence, and (b) they rely on experience and intuition. Because there is a large current situation, it was analyzed that appropriate processing was not necessarily performed in a timely manner.
Based on this analysis, the problems were examined. As a common issue for these germination treatments, the suitability of “stimulating the entire fungus bed” was cited. Then, assuming that this “stimulates the entire fungus bed”, the following concerns (a) and (b) were pointed out.
(A) When a treatment with a large stimulus is selected for a fungus bed in which the mushroom primordial is sufficiently prepared, the concentration of mushrooms is reduced and the fungus bed condition is deteriorated. Concerns arise.
Specifically, a) Inducing the occurrence from a place that does not want to be generated, b) The yield of mushrooms that can be shipped is reduced, and c) Mushrooms generated from the side and bottom of the fungal bed cannot be harvested in top cultivation. The fungus bed is damaged by rotting, and in addition, there is a possibility that the rotten mushroom attracts and proliferates harmful fungi and pests.
(B) On the contrary, when the preparation of the mushroom primordial is insufficient, or when a treatment with a small stimulus is selected for the fungus bed in which the amount of bacterial cells is reduced and the vitality is reduced, There is concern that mushrooms will not occur or that there will be little change.
Specifically, e) a decrease in yield and a decrease in the service life of the fungus bed can be mentioned.

Mushroom Cultivating Course Omori Kiyotosu and Koide Hiroshi Mushroom cultivation index Nagano Prefecture and others Revised Mushroom Cultivation Technology Plants World Co., Ltd. 2015 Summer Seminar Workshop Material National Sunmash Production Council, Kitaken Co., Ltd.

JP-A-2005-204603

  From the above, in the Shiitake germination treatment method, when the primordium is sufficiently prepared, local stimulation that is not the entire bacterial bed should be given with moderate stimulation while taking the whole into consideration. As a result of the inventor's consideration and exploration of a method to solve this problem, the inventors focused on the “existence of the coating” that is formed on the surface of the mycelium after the spread of the mycelia, and made an effort to utilize the existence of the coating. The present invention has been achieved.

The method for generating shiitake mushroom according to claim 1, wherein the film formed on the surface of the mycelium in the process of inoculating the shiitake fungus and incubating the mycelium with the spread of mycelia and culturing the mycelium, breaks the surface film and the mycelium layer below the surface film Germination is performed by piercing pores having a cross-sectional area of 0.5 to ²⁰ mm ^{2 that} reach up to.

The method for generating shiitake according to claim 2 is characterized in that the depth of the pores is set to 10 to 20 mm, and the distance between them is set to 2 to 6/10 ⁴ mm ² .

  The method for generating shiitake mushrooms according to claim 3 is characterized in that the pores are cut for 6 to 18 hours after sprouting treatment is performed by piercing the pores having a cross-sectional diameter of 1 to 5 mm that breaks the surface coating and reaches the mycelium layer below. It is characterized by urging water supply to the fungus bed.

  The method for generating shiitake mushroom according to claim 4, wherein the mycelium layer is formed by breaking the surface film and forming the mycelium layer below the film formed on the surface of the mycelium in the process of inoculating the shiitake fungus and incubating the mycelium by decomposing and decaying the medium. Germination is performed to cut a 5 to 10 mm long cut reaching up to.

The method for generating shiitake according to claim 5 is characterized in that the interval between the cuts is 2 to 6/10 ⁴ mm ² .

  The method for generating shiitake mushrooms according to claim 6, wherein the treatment is performed from 6 to 18 hours after breaking the surface coating and cutting a 5 to 10 mm long cut reaching the mycelium layer below the surface coating. It is characterized by prompting moisture supply.

According to the method for generating shiitake mushroom according to claim 1, an untreated product is obtained by carrying out a germination treatment by piercing a pore having a cross-sectional area of 0.5 to ²⁰ mm ² that breaks the surface coating and reaches the mycelium layer below it. Compared with, it was confirmed that the number of shiitake mushrooms increased, the fresh weight as a yield increased, and the value of the individual weight for ascertaining the size of one shiitake mushroom increased to an appropriate value.
As for the generation position, for example, in top cultivation, there was no generation from the side surface and bottom surface, and the result was that all occurred from the desired top surface.

  According to the method for generating shiitake mushrooms according to claim 2, the stimulation intensity of the pores in the germination treatment for piercing the pores is appropriate and the stimulation is local, but the entire bacterial bed is spaced at an appropriate interval. It was confirmed that

  According to the method for generating shiitake mushroom according to claim 3, by increasing the supply of moisture from the pores to the fungus bed from 6 hours to 18 hours after the germination treatment, an increase in the number of generations and yield is promoted, and the moisture content is increased. It was confirmed that the absorption time was shortened.

  According to the method for generating shiitake mushroom according to claim 4, a germination treatment is applied to break the surface coating and cut a length of 5 to 10 mm that reaches the mycelium layer below the surface coating, and is similar to the invention of claim 1. Thus, an increase in the number of shiitake mushrooms, an increase in yield, and an increase in single weight can be obtained, and the occurrence position is also from the desired position.

  According to the method for generating shiitake mushrooms according to claim 5, the intensity of the stimulation in the germination treatment for making a cut is appropriate, and the stimulation extends over the entire microbial bed at an appropriate interval while being local.

  According to the method for generating shiitake mushroom according to claim 6, by increasing the supply of moisture from the cut to the fungus bed from 6 hours to 18 hours after the germination treatment, the generation number and the yield are promoted, and the moisture absorption This shortens the time.

It is a chart figure which shows the flow of each process of this invention. It is a perspective view which shows the cultivation condition of this invention. It is a perspective view which shows what used the nail body by the specification used for this invention. It is a perspective view which shows what used the blade object by the specification used for this invention.

First, the film formation process which is the subject of the present invention will be described.
In the present invention, first, the medium is inoculated with shiitake mushrooms, and the medium undergoes a culturing process in which the medium is decomposed and decayed while the mycelium spreads. When the mycelium spreads throughout the medium, primordial formation is initiated in a part of the fungal bed. This primordium plays an important role in growing mushrooms by later germination from the mycelium layer, ripening to the fruit body through the juvenile body.
When this primordial formation starts, the formation of mycelium starts on the surface of the fungus bed in parallel with this, that is, small granular irregularities appear on the surface of the fungus bed, and the mycelium becomes an aggregate there Formation of lumps and hyphae begins. In many cases, the fungus bed surface is gradually colored brown at the same time as the formation of the primordia or at a slightly delayed rate, and eventually reaches a state of browning that is colored dark brown. Then, a certain layer of film is formed on this surface, and this becomes the “coating” that is the subject of the present invention.

Now, in the process of culturing, Shiitake does not end one fungus bed with the occurrence of a one-time mushroom, but reaps the germination of the grown mushrooms from the fungus bed and then gives germination stimuli to the fungus bed again. Grow the second mushroom. And the cultivation method which repeats this about 2 to 12 times is taken. That is, this is a method for generating mushrooms multiple times from one fungus bed.
In the case of multiple occurrences from this one fungus bed, as explained at the beginning, if no stimulation is given to the fungus bed and the primordium, germination becomes extremely low, and no garlic mushroom occurs, or The yield tends to be small and inefficient.

Therefore, in the present invention, as a solution to the above problem, attention will be paid to the “coating”. As described above, the coating is a mycelium mass on the surface of the microbial bed that is advanced in parallel with the formation of the primordium. It refers to a film that grows and is formed in a certain layer with a brown color change.
This film has a thickness of about 1 to 7 mm, and its hardness is not excessively hard, although it has a certain hardness as a film. For example, the surface of a softball sphere is relatively It becomes a thing with flexible elasticity.
This film is formed over the entire surface of the fungus bed.

The method for generating shiitake mushroom according to the present invention is to break the surface film against the film formed on the surface of the fungus bed in the process of inoculating the shiitake fungus and incubating the mycelium by decomposing and decaying the medium. A process of drilling pores having a cross-sectional area of 0.5 to ²⁰ mm ² reaching the lower mycelium layer is performed.
That is, the fungus bed at the time of germination is subjected to a treatment that pierces the surface of the film formed in the above process and breaks the film to reach the mycelium layer below it, thereby stimulating the germination (This is hereinafter referred to as pore germination treatment).
The pores are pores having a cross-sectional area of 0.5 to ²⁰ mm ² (corresponding to a diameter of about 1 to 5 mm), and a pore having a depth that reaches the mycelium layer below it after breaking the coating. To do.
The hole is typically a circular hole, but is not limited thereto, and includes a hole having an elliptical shape, a triangular shape, a quadrangular shape, a star shape, or the like in cross-sectional shape.

Specifically, as shown in FIG. 3, for example, six nail bodies 1a having a diameter of 2 mm and a length of about 150 mm are prepared, and these are arranged on a plate-like board body 2 of 170 mm × 90 mm at substantially equal intervals. Prepare Spec 3 that is distributed and struck.
Since the degree of dispersion is 6 in an area of 170 mm × 90 mm, 6 / 1.53 × 10 ⁴ mm ² is obtained. When this is converted into an area of 100 mm × 100 mm, it is about 4/10 ⁴ mm ² .

Using such specifications, the nail body is pierced from the upper surface of the fungus bed with respect to the film that has reached the above-described stage, and the tip thereof is made to reach a depth of about 15 mm of the mycelium layer under the film.
When the nail body is confirmed to be pierced, it is gently pulled out to return to the original fungus bed state.
As a result, pores having a cross-sectional diameter of 2 mm and a depth of 15 mm are formed in the surface coating of the fungus bed.
The vertical movement of this specification may be performed either by using a lifting device or by a human hand.

When performing a germination process for making a cut (hereinafter referred to as a cut germination process), a blade object (for example, a knife) with a sharp tip is used instead of the nail body, and its length is 5 to 10 mm. Can do.
Specifically, as shown in FIG. 4, six blade objects 1b having a width of 8 mm, a thickness of 0.6 mm, and a length of 150 mm are equally spaced on a plate-like plate body 2 of 170 mm × 90 mm as in the case of the nail body. Use Spec 3 standing in a distributed manner.
The outline is the same as that of the nail body.

Next, after performing the pore germination treatment, an operation for urging the supply of moisture from the pores to the fungus bed is performed for 6 to 18 hours as necessary.
In other words, in the cultivation of shiitake mushrooms, water must be supplied at any point in time between occurrence and harvest, but this water supply is not always sufficient, or the water supply takes longer than 24 hours. It took time.
Therefore, when this water supply is performed at the same time as the above-mentioned pore germination treatment, a desired result can be obtained depending on the yield and single weight.
More specifically, about 400 ml of water is stored in a fruit pack, and the fungus bed is turned upside down with respect to the fungus bed that has been subjected to pore germination treatment or cut germination treatment in the top surface cultivation. That is, it is immersed in a form in which the surface subjected to the pore germination treatment faces downward.
This treatment is preferably performed within 20 hours, preferably between 6 hours and 18 hours, in order to shorten the time for water supply.

Through the pore germination process and the water supply operation, a young fruit body is born in the fungus bed, grows into a fruit body, and proceeds to maturation of the mushroom, and the first trimming is finished.
Then, by applying germination stimulation to the fungus bed again, the second and subsequent multiple mushroom harvests are promoted. At this time, the above-described germination treatment of the present invention is added.
The intervals at which the germination treatment of the present invention is applied are not necessarily performed each time a plurality of times, and the number and interval are determined according to changes in the variety and environment.

The cut germination treatment is exactly the same as the pore germination treatment.

Next, functions and effects of the present invention will be described.
By the germination process by piercing the pores with a cross-sectional area of 0.5 to ²⁰ mm ² that breaks the surface coating and reaches the mycelium layer below the surface by the Shiitake generation method, Shiitake It is possible to increase the number of occurrences, increase the raw weight as a yield, and increase the value of the individual weight asking for the size of a single shiitake. This was confirmed by Example 1 described later.
This is because a part of the coated cell is destroyed by applying a treatment to pierce the pores, and outside air is introduced from the vicinity of the pores, and the germination reaction is activated as a defense reaction against these stimuli. Inferred.

In addition, the time when the coating formed on the surface of the fungus bed in the above process turns brown, even under the time conditions, corresponds to a state in which the primordial is sufficiently prepared, and preparation for germination is promoted by some stimulus Is considered a state of being arranged.

In addition, by applying a treatment that breaks the surface coating and pierces the pores that reach the mycelium layer below it, it is a local stimulus that does not uniformly stimulate the entire mycelium bed, and the size of the pores By making a hole with a cross-sectional area of 0.5 to ²⁰ mm ² , the stimulation is not too strong or too weak, avoiding the disadvantage of becoming a single piece due to concentration occurrence, The market value was large.

As for the generation position, in the top surface cultivation, there is no generation from the side surface and the bottom surface, and everything is generated from the desired top surface. This was confirmed by Example 2.
This is presumed to occur from the upper surface as the desired position in response to the stimulus if given from the upper surface direction in the case where the stimulus is desired, for example, in the case of upper surface cultivation.

In addition, it was confirmed that the individual weight would be appropriate by setting the depth of the pores to 10 to 20 mm and the mutual interval to 2 to 6/10 ⁴ mm ² .
This is because the stimuli themselves are local, but they are kept at an appropriate distance from each other, so that it is presumed that the stimuli are properly distributed throughout and the single weight is increased.

Further, by promoting the supply of moisture from the pores to the fungus bed 6 to 18 hours after the germination treatment, the generation number and the yield are promoted, and the moisture absorption time is shortened. . This was confirmed by Example 3.
This is because if the above germination treatment breaks the surface coating and pierces the pores that reach the underlying mycelium layer, water can easily enter through the path, and it penetrates into the mycelium layer. In a short time, sufficient water has been supplied.

Regarding this action and effect, in the case of the break germination treatment, it is exactly the same as the pore germination treatment.

In this example, the film was subjected to pore germination treatment, and the number of mushrooms generated, fresh weight, individual weight, etc. were measured, and the effectiveness of the treatment was confirmed.
First, the composition of the medium is a mixture of corn chips and sawdust mixed at a volume ratio of 6: 4, and a New Videl (Shitaken Co., Ltd.) for short-term cultivation of shiitake mushrooms is added as a nutrient to the medium. % And high-pressure sterilization (118 ° C., 60 minutes) was performed. After cooling, the cultivar was inoculated with Kitaken 705 (Kitaken Co., Ltd.) and cultured at 20 ° C. for 120 days. About the outbreak, the top cultivation which raise | generates a mushroom from the upper surface of a fungus bed was performed, and generation | occurrence | production management was made into 6 months.
Under the above conditions, a control zone and a treatment zone were set. In the control group, the germination stimulation on the fungus bed was made only by the temperature change of the side water, and C1 was made by submerging the fungus bed in a fruit pack containing water in addition to this temperature change and submerging the inside. . On the other hand, the one subjected to the above-mentioned pore germination treatment was defined as a treatment section, and the one that promoted the generation of mushrooms from the virgin medium (the one that occurred most) was designated as A1, and the first occurrence was promoted to the second occurrence. (Corresponding to No. 4) was designated as A2, and similarly, equivalent to the sixth time (corresponding to No. 6) was designated as A3. The number of fungus beds was 10 in each group. The process of drilling the pores is to create a spec with rods (nails) with a pointed tip of 2 mm in diameter and 150 mm in length, planted on a 170 mm x 90 mm plate at six intervals. This was done by drilling pores on the surface of the fungus bed.
The number of mushrooms generated, the fresh weight as the weight, and the individual weight as the weight per one of the above specimens were measured.
In addition, the specs for performing the pore germination treatment shown in the examples are those in which six nails having a cross-sectional diameter of 2 mm and a length of 150 mm are dispersed on a plate-like disk body of 170 mm × 90 mm at equal intervals. Using.

The results were as shown in Table 1 below.

Compared to the C1 ward, where germination was promoted only by a temperature change that was a mild stimulus, the number of mushrooms increased and the yield was the highest in the C2 ward subjected to fruit pack water immersion. However, there are many cases where the occurrence is concentrated locally, and the raw weight as a weight per piece has decreased.
On the other hand, in the A1 to A3 districts subjected to the treatment of the present invention, the number of mushrooms generated and the raw weight as a yield increased, and the mushroom individual weight also increased. That is, it was confirmed that not only the number of mushrooms generated and the overall yield increased, but also the weight per piece increased. In addition, there was a tendency for the occurrence to occur on average without concentrating the occurrence locally over the entire generation period.
When comparing the A1 to A3 wards, the number and fresh weight increased most in the A3 ward, and there was a tendency for the occurrence to be slightly concentrated in the later stage while the yield fluctuation was slightly large in the early to middle period. Comparing each other, the A3 ward was suitable. However, this time, the Hakken 705 was used as a specimen, but when different varieties were used, the suitable timing may be different, and it is not always 6 It is considered that it is not possible to judge that the number generation is suitable.

Based on the treatment of the present invention, the effect of promoting germination from a site where mushroom generation is desired was examined.
The culture is the same as in Example 1. The composition of the medium is a mixture of corn chips and sawdust in a volume ratio of 6: 4. % Was added, the water content was adjusted to 60%, and high-pressure sterilization (118 ° C., 60 minutes) was performed. After cooling, the cultivar was inoculated with Kitaken 705 (Kitaken Co., Ltd.) and cultured at 20 ° C. for 120 days.
About generation, we perform top surface cultivation to generate from the upper surface of fungus bed and want to germinate only by temperature change with respect to the fungus bed after the end of the first occurrence, we want to tap the fungus bed by hand C2 is the basin area that stimulates, C3 area is the reverse drainage water area that drains the water accumulated between the fungus bed and the cultivation bag, and the water is supplied again. The partial water immersion immersed in the fruit pack was designated as C4.
The pore germination treatment was the same as in Example 1 above.
The number of occurrences of mushrooms in each section from the upper surface and other locations was measured when the location where mushroom generation was desired was defined as the upper surface.

The results were as shown in Table 2 below.

As a result, in the control zone (C1) with only the temperature change, the basin zone (C2), the inverted drainage water (C3), and the partial flooding (C4) using the fruit pack, germination such as the side and bottom of the fungus bed is not desired. Occurrence from the location accounted for 12%, 41%, 44% and 25%, respectively. Moreover, in the (C3) and (C4) sections, there was a tendency that the labor required for the processing was large.
On the other hand, in the pore germination treatment section (A) of the present invention, it was found that all occurrences were obtained from the desired upper surface, and the occurrence from the desired location was 100%. In addition, the labor is small.

(A)
Although the effectiveness of the pore germination treatment could be confirmed by Example 2, it was verified what effect to the occurrence of mushrooms, harvesting, etc., when “the operation of supplying moisture to the fungus bed” was added thereto.
In other words, when cultivating Shiitake mushrooms, water must be supplied in a timely manner at any point in the generation period, but if this water supply is performed at the same time as the above-mentioned pore germination treatment, depending on the yield and single weight, etc. We anticipated that the desired results would be obtained and decided to verify this.

First, after performing the pore germination treatment, when performing the “water supply operation”, it was determined how much moisture the bacteria bed that had undergone the pore germination treatment absorbed.
Shiitake culture conditions were the same as in Example 1 above, and the medium composition was a mixture of Quercus chips and sawdust mixed at a volume ratio of 6: 4. The finished weight ratio was 10% added, the water content was adjusted to 60%, and high-pressure sterilization (118 ° C., 60 minutes) was performed. After standing to cool, the cultivar was inoculated with Kitaken 705 (Kitaken Co., Ltd.) and cultured at 20 ° C. for 120 days.
The generation conditions were such that 13 ° C. and 22 ° C. were changed alternately for 12 hours, and germination stimulation was performed by water immersion (4 hours). The untreated control group (C) and the treated group (A) where the treatment was performed using the bacterial bed after the first occurrence was set (10 samples of each bacterial bed were tested), and sprinkling management was performed twice a day 30 The measurement was performed for 3 days, and the change in the bacterial bed weight was measured.

The results were as shown in Table 3 below.

As a result, the weight increase of the untreated control group (C) was 0.98% in the treated group (A) compared to the control group (C), whereas the treated group (A) was 4. The increase was 04%, and a significant increase in the bacterial bed weight was observed.
Therefore, first, it was confirmed that the moisture was sufficiently absorbed in the bacteria bed by the pore germination treatment and the water supply operation.

(B) Based on the above (A), it was confirmed that sufficient moisture was absorbed into the fungus bed by the pore germination treatment, but what kind of influence was exerted on the yield of mushrooms by the fungus bed in which sufficient moisture was absorbed. I verified it.
The fungus bed used was Kitaken No. 705 as in the previous example. After the first occurrence, rest management (23 ° C, 7 days) is performed, and the control sections (C1) and (C2) where no treatment is performed and the treatment sections (A1) and (A2) in which submerged operation is added to the pore germination treatment are set. did. The operation time was 6 hours for (C1) and (A1) and 18 hours for (C2) and (A2).

The results were as shown in Table 4 below.

As a result, in 6 hours operation, the difference in the weight of the fungus bed was 55 g before and after the pack soaking in the C1 ward without treatment, and the mushroom yield was 192 g / fungus bed. In the A1 district with the addition of water, the difference in the weight of the fungus bed before and after the flooding of the pack was 70 g, and the yield of mushrooms was 192 g / fungus bed. In the 18-hour treatment, the difference in the weight of the fungus bed was 80 g before and after pack inundation in the C2 district without treatment, and the yield of mushrooms was 322 g / fungus bed, whereas in the A2 district where the treatment was carried out, the pack was flooded. The difference in the weight of the fungus bed between before and after was 168 g, and the yield of mushrooms was 349 g / fungus bed.
That is, when comparing the untreated control group and the treated group, the treated areas A1 and A2 tend to increase the amount of water absorption and increase the mushroom yield compared to the untreated C1 and C2. Admitted. Further, when the operation times were compared, in the A2 section where the operation time was 18 hours compared to the A1 section where the operation was performed for 6 hours, the A2 section where the operation time was long was higher in both the water absorption amount and the yield.
Therefore, in order to increase both the amount of water absorption and the yield, it is desirable to perform the treatment and keep the operation time as long as possible.
On the other hand, regarding the operation time, A2 ward operated for 18 hours was 349 g / bacteria bed, whereas A1 ward operated for 6 hours became 298 g / bacteria bed, and the yield of A1 ward was about 85% of A2 ward. It was something to reach.
From this, it was found that if a water immersion operation for 6 hours or more is performed, an effective and relatively high water absorption amount and yield can be secured.
From here, by setting the immersion time (water supply time) from 6 hours to 18 hours, a highly effective water absorption amount and yield can be expected.
The treatment time of 6 hours and 18 hours described above requires 24 hours or more in the case of water immersion in normal mushroom cultivation, and from this point, the addition of the water immersion operation to the pore germination treatment shortens the time and increases the yield. It is confirmed that there is an effect.

  In Shiitake fungus bed cultivation, the use of existing methods, as well as factory production that requires greater reductions in labor costs, facility costs, etc., enables efficient and effective pore germination treatment by using the present invention. Can be performed.

1a ··· Nail 1b · · Blade object 2 · Plate disc 3 · · Spec 3 · · Medium

Claims (6)

In contrast to the film formed on the surface of the mycelium in the process of inoculating shiitake fungi and decomposing and decaying the medium with the spread of mycelia, the cross-sectional area that breaks the surface film and reaches the mycelium layer below it is 0.5 to ²⁰ mm ² A method for generating shiitake mushrooms, characterized by performing a germination treatment to pierce the pores. ^{2. The} method for generating shiitake mushrooms according to claim 1, wherein the depth of the pores is 10 to 20 mm, and the interval between the pores is 2 to 6/10 ⁴ mm ² . After performing a germination treatment for piercing a pore having a cross-sectional area of 0.5 to ²⁰ mm ² that breaks the surface coating and reaches the mycelium layer below it, water supply from the pore to the mycelium is promoted from 6 to 18 hours. The method for generating shiitake mushrooms according to claim 1 or 2.   In the process of inoculating shiitake fungus and culturing by decomposing and decaying the medium with the spread of mycelia, the cut formed on the surface of the mycelium is 5 to 10 mm long to break the surface film and reach the mycelium layer below it A method for generating shiitake mushrooms, characterized by performing a germination treatment. 5. The method for generating shiitake mushrooms according to claim 4, wherein the interval between the cuts is set to 2 to 6 pieces / 10 ⁴ mm ² .   A water supply is urged from 6 to 18 hours from the cut to the fungus bed after the germination treatment for breaking the surface coat to reach the mycelium layer below it is performed. Item 5. A method for generating shiitake mushrooms according to item 5.

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