CN105874966A - Method for promoting germination of Heritiera plant seeds - Google Patents
Method for promoting germination of Heritiera plant seeds Download PDFInfo
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- CN105874966A CN105874966A CN201610280732.XA CN201610280732A CN105874966A CN 105874966 A CN105874966 A CN 105874966A CN 201610280732 A CN201610280732 A CN 201610280732A CN 105874966 A CN105874966 A CN 105874966A
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- germination
- seed
- seeds
- leucadendron
- plant seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for promoting germination of Heritiera plant seeds. The method includes the steps of firstly, performing scarifying treatment on Heritiera plant seed shells; cutting off a small amount of seed coats to expose part of white endosperm; secondly, soaking for accelerating germination, to be more specific, soaking the seeds after sterile treatment into a gibberellin-containing sterile solution, and placing in a dark cultivation box for accelerating germination; thirdly, subjecting the seeds to in-vitro culture to induce germination, to be more specific, under a sterile condition, inoculating the seeds after soaking for accelerating germination on an MS culture medium, and performing in-vitro culture at variable temperature for 20-50 days to enable the seeds to germinate gradually. By using the method, germination rate of the Heritiera plant seeds can be effectively increased, and seed germination time can be shortened.
Description
Technical field
The present invention relates to a kind of method promoting plant seed germination, particularly relate to a kind of promotion Leucadendron plant seed
The method sprouted.
Background technology
Originate in Proteaceae (Proteaceae) Leucadendron (Leucadendron) plant in South Africa, cut for top grade
Leaf cut-flower, potted plant and flower garden are viewed and admired, and are the most excellent ornamencal flower and trees.But seed is difficult to sprout under normal conditions, exists
Dormancy.Release seed dormancy, improve the seed germination planting percent of Leucadendron (Leucadendron) plant, be to improve silver
Leaf Pterostyrax plant introduction and the important content of Lycopersicum esculentum efficiency.Under conventional germination condition, Leucadendron plant seed germination rate
It is 0.
Summary of the invention
The present invention proposes a kind of method promoting Leucadendron plant seed germination, and the method can improve Leucadendron and plant
Thing percentage of seedgermination and shortening seed germination time, improve seed seedling efficiency.
The technical scheme is that and be achieved in that: a kind of method promoting Leucadendron plant seed germination, including
Following steps:
Step one, processes Leucadendron plant seed kind shell broken skin: cut a small amount of seed coat, exposed portion white endosperm；
Step 2, Steeping and budding processes: is immersed in sterile solution after being processed by seed asepsis, is positioned over dark culturing case
Interior accelerating germination processes；
Step 3, seed isolated culture sprout-induction: aseptically, the seed after being processed by Steeping and budding is seeded in
In MS culture medium, isolated culture, within 20～50 days, sprout successively.
Further, in step 2, the temperature of dark culturing case is 22-28 DEG C.
Further, the method that in step 3, in-vitro culture method uses alternating temperature isolated culture: every day 18-22 DEG C cultivates 8-
Cultivate 14-16h for 10h, 8-12 DEG C.
Further, the sterile solution in step 2 is gibberellins sterile solution.
Further, the mass concentration of described gibberellins sterile solution is 0.05-0.15g/L.
The invention have the benefit that the method promoting Leucadendron plant seed germination of the present invention can be effectively increased
Leucadendron plant seed germination rate and shortening seed germination time.
Detailed description of the invention
For being more fully understood that the present invention, below by following example the present invention made the most concrete elaboration, but not
Can be regarded as limitation of the invention, some made according to foregoing invention content for those skilled in the art are nonessential
Improve and adjust, be also considered as being within the scope of the present invention.
Use Leucadendron plant seed Leucadendron linifolium, Leucadendron from South Africa
Conicum and Leucadendron laureolum.
Embodiment one
After three kinds of seeds are carried out surface sterilization sterilizing, it is soaked in aseptic aqueous solution, is placed in 25 DEG C of dark culturing casees
Accelerating germination processes；Aseptically, the seed after being processed by Steeping and budding is seeded in MS culture medium, 25 DEG C of in vitro trainings of constant temperature
Support, wait and sprouting.Inoculate 15 seeds, if 2 times are repeated.
Embodiment two
Three kinds of seeds are prescinded small part seed coat in anatomical lens incision, does not injure embryo for degree to expose white endosperm；Will
After seed carries out surface sterilization sterilizing, being soaked in aseptic aqueous solution, in being placed in 25 DEG C of dark culturing casees, accelerating germination processes；Aseptic
Under the conditions of, the seed after being processed by Steeping and budding is seeded in MS culture medium, 25 DEG C of isolated culture of constant temperature, waits and sprouting.Inoculation
15 seeds, if 2 times are repeated.
Embodiment three
Three kinds of seeds are prescinded small part seed coat in anatomical lens incision, does not injure embryo for degree to expose white endosperm；Will
After seed carries out surface sterilization sterilizing, it is soaked in containing in 0.10g/L gibberellins (GA) sterile solution, is placed in 25 DEG C of dark culturing
In case, accelerating germination processes；Aseptically, the seed after being processed by Steeping and budding is seeded in MS culture medium, and constant temperature 25 DEG C is in vitro
Cultivate, wait and sprouting.Inoculate 15 seeds, if 2 times are repeated.
Embodiment four
Three kinds of seeds are prescinded small part seed coat in anatomical lens incision, with expose white endosperm but do not injure embryo for degree (with
Do not cut seed coat seed for comparison)；After seed is carried out surface sterilization sterilizing, it is soaked in containing 0.10g/L gibberellins (GA) nothing
In bacterium solution, in being placed in 25 DEG C of dark culturing casees, accelerating germination processes；Aseptically, the seed inoculation after Steeping and budding being processed
In MS culture medium, isolated culture under alternating temperature, wait and sprouting, the method for Fluctuation temperature culture is: cultivate 10h, 10 DEG C of cultivations for 20 DEG C
14h.Inoculate 15 seeds, if 2 times are repeated.
Record weekly the percentage of seedgermination of above-described embodiment one to four, and statistic record Average Germination Days.In in vitro training
Germination percentage after supporting 50 days and Average Germination Days statistics, see table 1.
Table 1 Leucadendron plant seed germination rate and germination natural law
Note: "-" refers to that this process no data that do not germinates presents.
As known from Table 1, seed coat cutting process contributes to Leucadendron plant seed germination, sprouts than the seed not cutting seed coat
The rate of sending out increases；Gibberellins GA Steeping and budding, also by stratification, soaks 24h under the conditions of 25 DEG C and carries out accelerating germination process, plant
Sub-germination percentage improves 21%-25%, and germinating time shortens 5-12 days.Therefore, gibberellins GA Steeping and budding after seed coat cutting
Contribute to Leucadendron plant seed improve germination percentage and shorten germinating time.
Under isolated culture condition, Fluctuation temperature culture (10h, 14h at 10 DEG C at every day 20 DEG C) compared with 25 DEG C of constant temperature culture,
Significantly improve Leucadendron plant seed germination rate and shorten germinating time.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (5)
1. the method promoting Leucadendron plant seed germination, it is characterised in that comprise the following steps:
Step one, processes Leucadendron plant seed kind shell broken skin: cut a small amount of seed coat, exposed portion white endosperm；
Step 2, Steeping and budding processes: is immersed in sterile solution after being processed by seed asepsis, urges in being positioned over dark culturing case
Bud processes；
Step 3, seed isolated culture sprout-induction: aseptically, the seed after being processed by Steeping and budding is seeded in MS training
Support on base, isolated culture, within 20～50 days, sprout successively.
2. the method promoting Leucadendron plant seed germination as claimed in claim 1, it is characterised in that: dark in step 2
The temperature of incubator is 22-28 DEG C.
3. the method promoting Leucadendron plant seed germination as claimed in claim 1, it is characterised in that in vitro in step 3
Cultural method uses the method for alternating temperature isolated culture: cultivates 8-10h every day 18-22 DEG C, cultivates 14-16h for 8-12 DEG C.
4. the method promoting Leucadendron plant seed germination as claimed in claim 1, it is characterised in that: the nothing in step 2
Bacterium solution is gibberellins sterile solution.
5. the as claimed in claim 5 method promoting Leucadendron plant seed germination, it is characterised in that: described gibberellins without
The mass concentration of bacterium solution is 0.05-0.15g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610280732.XA CN105874966B (en) | 2016-04-29 | 2016-04-29 | A method of promoting Leucadendron plant seed germination |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610280732.XA CN105874966B (en) | 2016-04-29 | 2016-04-29 | A method of promoting Leucadendron plant seed germination |
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| Publication Number | Publication Date |
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| CN105874966A true CN105874966A (en) | 2016-08-24 |
| CN105874966B CN105874966B (en) | 2019-04-05 |
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| CN201610280732.XA Active CN105874966B (en) | 2016-04-29 | 2016-04-29 | A method of promoting Leucadendron plant seed germination |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184595A (en) * | 2018-02-26 | 2018-06-22 | 濮阳市农业科学院 | A kind of cultural method of high oleic acid peanut |
| CN112997787A (en) * | 2021-02-07 | 2021-06-22 | 广东红树林生态科技有限公司 | Rapid seedling raising method for half-mangrove plant silver leaf tree |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100797980B1 (en) * | 2006-10-12 | 2008-01-28 | 주식회사 태림 | The chip of small size seed and the method of preparing it |
| CN101524042A (en) * | 2008-12-03 | 2009-09-09 | 广东海洋大学 | Fruit seedling cultivating technology of looking-glass trees |
-
2016
- 2016-04-29 CN CN201610280732.XA patent/CN105874966B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100797980B1 (en) * | 2006-10-12 | 2008-01-28 | 주식회사 태림 | The chip of small size seed and the method of preparing it |
| CN101524042A (en) * | 2008-12-03 | 2009-09-09 | 广东海洋大学 | Fruit seedling cultivating technology of looking-glass trees |
Non-Patent Citations (4)
| Title |
|---|
| 曾力: "六种山龙眼科木本切花植物引种繁殖的研究", 《西南林学院硕士学位论文》 * |
| 李红燕 等: "不同处理对木球卢卡树种子出苗的影响", 《山东林业科技》 * |
| 罗昌国 等: "不同处理对山龙眼花卉红花卢卡树种子的发芽影响", 《种子》 * |
| 高秀梅 等: "银叶树育苗试验研究", 《福建林业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184595A (en) * | 2018-02-26 | 2018-06-22 | 濮阳市农业科学院 | A kind of cultural method of high oleic acid peanut |
| CN112997787A (en) * | 2021-02-07 | 2021-06-22 | 广东红树林生态科技有限公司 | Rapid seedling raising method for half-mangrove plant silver leaf tree |
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| CN105874966B (en) | 2019-04-05 |
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