CN106166453A - A kind of passive mixing device for high performance liquid chromatography - Google Patents
A kind of passive mixing device for high performance liquid chromatography Download PDFInfo
- Publication number
- CN106166453A CN106166453A CN201610527610.6A CN201610527610A CN106166453A CN 106166453 A CN106166453 A CN 106166453A CN 201610527610 A CN201610527610 A CN 201610527610A CN 106166453 A CN106166453 A CN 106166453A
- Authority
- CN
- China
- Prior art keywords
- mixing unit
- hole
- upper cover
- disc
- thread
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/40—Static mixers
- B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
- B01F25/421—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions by moving the components in a convoluted or labyrinthine path
- B01F25/423—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions by moving the components in a convoluted or labyrinthine path by means of elements placed in the receptacle for moving or guiding the components
- B01F25/4232—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions by moving the components in a convoluted or labyrinthine path by means of elements placed in the receptacle for moving or guiding the components using dams
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/45—Mixing liquids with liquids; Emulsifying using flow mixing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/716—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/36—Control of physical parameters of the fluid carrier in high pressure liquid systems
Abstract
The invention discloses one for the passive mixing device of high performance liquid chromatography, the branch utilizing liquid stream converges high efficiency mixing and the uniformly mixing realizing flowing phase again, including thread mixing unit, the end face of thread mixing unit compresses and is sealed and installed with upper cover, and the bottom surface of thread mixing unit compresses and is sealed and installed with lower cover.The present invention can realize the mixing of high efficiency and high evenness.The dead volume of whole system stream is the least simultaneously, can be effectively improved the performance of gradient elution.
Description
Technical field
The present invention relates to liquid chromatograph fields of measurement, a kind of passive mixing device for high performance liquid chromatography.
Background technology
High performance liquid chromatography is an important branch of chromatographic isolation measurement technology.High performance liquid chromatograph is carried out
During gradient analysis, the concentration of flowing phase can persistently change or stepped change.In gradient analysis, need to utilize blender
The flowing of two or more is mixed the most uniformly, if mixing efficiency is not enough, i.e. when multiple flowing is mutually by detector
There is no mix homogeneously, repeatability and the accuracy of detector analysis result will be affected.Therefore add between pump and chromatographic column
Blender, mixes uniformly realizing high efficiency and height.Existing high performance liquid chromatography blender because of technology maturation with become we
Face considers, many employings passive mixing (static mixing) mode.Passive mixer concrete implementation form includes disturbing in flow line
Stream mixing, chaos formula mixes, and branch converges formula mixing etc. again.It is big all to there is dead volume in the blender of a lot of designs, mix homogeneously
The shortcoming such as property is undesirable.
Summary of the invention it is an object of the invention to provide a kind of passive mixing device for high performance liquid chromatography, to solve
Prior art blender dead volume is big, the problem that mixing uniformity is undesirable.
In order to achieve the above object, the technical solution adopted in the present invention is:
A kind of passive mixing device for high performance liquid chromatography, it is characterised in that: utilize the branch of liquid stream to converge realization stream again
The high efficiency mixing of dynamic phase and uniformly mixing, including thread mixing unit, the end face of thread mixing unit compresses and is sealed and installed with
Upper cover, the bottom surface of thread mixing unit compresses and is sealed and installed with lower cover, wherein:
Described thread mixing unit includes thread mixing unit disc, thread mixing unit disc center be provided with as in
The central through hole of heart mixed zone, the end face of thread mixing unit disc, bottom surface be provided with the circular groove as drainage trough the most with one heart,
Thread mixing unit disc end face, bottom surface further respectively have multiple tracks in corresponding surface uniform ring around the spiral channel of central through hole,
Spiral channel is as spiral flow path, and each spiral flow path one end notch is respectively communicated to the drainage trough of correspondence, each spiral flow path
Other end notch is respectively communicated to central mixing zone, and each spiral flow path degree of depth is line from drainage trough to direction, central mixing zone
Property increase；
Described upper cover includes that upper cover disc, upper cover disc end face are coaxially provided with the conical surface, are provided with straight down from conical surface end face
The through conical surface, the central through hole of upper cover disc, the conical surface is provided with liquid input through hole, and multiple liquid input through hole is the most equal
Even being distributed in around conical surface central shaft, and multiple liquid input through hole tilts the through conical surface, upper cover disc, upper cover passes through bottom surface
When compression is sealingly mounted at thread mixing unit end face, the central through hole of upper cover connects with thread mixing unit center mixed zone,
The liquid input aperture, through hole lower end of upper cover is respectively communicated to the drainage trough of thread mixing unit；
Described lower cover includes lower cover disc, is eccentrically provided with through hole in lower cover disc, and described lower cover compression is sealed in spiral and mixes
When closing unit bottom surface, in lower cover, aperture, through hole upper end is communicated to the drainage trough of thread mixing unit.
Described a kind of passive mixing device for high performance liquid chromatography, it is characterised in that: described thread mixing unit
In, the spiral flow path of thread mixing unit disc end face and bottom surface is in being alternately distributed.
Described a kind of passive mixing device for high performance liquid chromatography, it is characterised in that: upper cover and lower cover can be mutual
Replace and use, meet different mode of operation demands with the flow channel of realization collocation varying number.
The present invention discloses the structure of a kind of new passive mixing device, utilizes the pressure branch of liquid stream to converge realization flowing again
The high efficiency mixing of phase and uniformly mixing, it is possible to achieve high efficiency and the mixing of high evenness.Whole system stream is dead simultaneously
Volume the least (less than 100uL), can be effectively improved the performance of gradient elution.
Accompanying drawing explanation
Fig. 1 is superstructure schematic diagram of the present invention, wherein:
Fig. 1 a is top view, and Fig. 1 b is upward view.
Fig. 2 is thread mixing cellular construction schematic diagram of the present invention, wherein:
Fig. 2 a is top view, and Fig. 2 b is sectional view.
Fig. 3 is lower cover structure schematic diagram of the present invention, wherein:
Fig. 3 a is top view, and Fig. 3 b is upward view.
Fig. 4 is 1 time overall structure flow circuit diagram of mode of operation in detailed description of the invention.
Fig. 5 is 2 times overall structure flow circuit diagrams of operational module in detailed description of the invention.
Detailed description of the invention
As Figure 1-Figure 5, a kind of passive mixing device for high performance liquid chromatography, utilize the branch of liquid stream to converge again
Realizing high efficiency mixing and the uniformly mixing of flowing phase, including thread mixing unit 1, the end face of thread mixing unit 1 compresses close
Envelope is provided with upper cover 2, and the bottom surface of thread mixing unit 1 compresses and is sealed and installed with lower cover 3, wherein:
Thread mixing unit 1 includes thread mixing unit disc 1.1, and thread mixing unit disc 1.1 center is provided with work
Centered by the central through hole of mixed zone 1.2, the end face of thread mixing unit disc 1.1, bottom surface are provided with the most with one heart as drawing
The circular groove of chute 1.3, thread mixing unit disc 1.1 end face, bottom surface further respectively have multiple tracks corresponding surface uniform ring around in
The spiral channel of heart through hole, spiral channel is as spiral flow path, and it is right that 1.4 each spiral flow path 1.4 one end notches are respectively communicated to
The drainage trough 1.3 answered, each spiral flow path 1.4 other end notch is respectively communicated to central mixing zone 1.2, and each spiral flow path
1.4 degree of depth linearly increase from direction, drainage trough 1.3 to central mixing zone 1.2；
Upper cover 2 includes that upper cover disc 2.1, upper cover disc 2.1 end face are coaxially provided with the conical surface 2.2, vertical from the conical surface 2.2 end face
To having the central through hole 2.3 of the through conical surface 2.2, upper cover disc 2.1, the conical surface 2.2 is provided with liquid input through hole 2.4,
Multiple liquid input through hole 2.4 is circularly and evenly distributed around the conical surface 2.2 central shaft, and multiple liquid input through hole 2.4 inclines
The oblique through conical surface 2.2, upper cover disc 2.1, upper cover 2 is compressed by bottom surface when being sealingly mounted at thread mixing unit 1 end face, on
The central through hole 2.3 of lid 2 connects with thread mixing unit 1 central mixing zone 1.2, liquid input 2.4 times stomidiums of through hole of upper cover 2
Mouth is respectively communicated to the drainage trough 1.3 of thread mixing unit 1；
Lower cover 3 includes lower cover disc 3.1, is eccentrically provided with through hole 3.2 in lower cover disc 3.1, and lower cover 3 compresses and is sealed in spiral
During mixed cell 1 bottom surface, in lower cover 3, aperture, through hole 3.2 upper end is communicated to the drainage trough 1.3 of thread mixing unit 1.
In thread mixing unit 1, the spiral flow path 1.4 of thread mixing unit disc 1.1 end face and bottom surface is in alternately dividing
Cloth.
In thread mixing unit 1, liquid arrives spiral flow path through drainage trough 1.3, mixes with center in central mixing zone 1.2
Tangent plane direction, district becomes 30 degree to import central mixing zone.
Upper cover 2 and lower cover 3 can mutually replace use, meet different work with the flow channel of realization collocation varying number
Mode requirement.
Specific embodiment:
In the present invention, comprise the upper cover of liquid stream input, thread mixing unit and lower cover three part comprising liquid stream output and form.
Being stamped a conical surface design on as it is shown in figure 1, be circularly and evenly distributed in the conical surface four liquid input through holes,
The liquid to be mixed that four tunnels are different can be accessed.Liquid to be mixed is pooled to thread mixing unit by liquid input through hole.On
One central through hole of lid Center is passed directly into the central mixing zone of thread mixing unit.
As in figure 2 it is shown, thread mixing unit contact sealing by compressing mode with upper cover, the end face of thread mixing unit with
Bottom surface connects as four liquid input through holes of the circular groove of drainage trough, circular groove and upper cover, guides liquid to be mixed to enter mixing
Spiral flow path, spiral flow path groove width 0.5mm, the degree of depth of groove is linearly increasing from drainage trough to central mixing zone.Central mixing zone
For cylindrical hole, volume about 50uL.Spiral flow path totally ten four tunnel, is respectively arranged with seven tunnels and is evenly distributed on the end face of thread mixing unit
And bottom surface.The drainage trough of end face, bottom surface is connected to central mixing zone by spiral flow path respectively.At the sidewall of central mixing zone, come
It is alternately distributed from the spiral flow path of end face, bottom surface.Liquid arrives spiral flow path through drainage trough, at central mixing zone and incident cylinder
Tangent plane direction becomes 30 degree to import central mixing zone.Alternatively distributed incident stream makes incident liquid stream reach maximum in central mixing zone
Mixing efficiency.
As it is shown on figure 3, lower cover contacts sealing with the back side of thread mixing unit by compression mode.Thread mixing unit
Back side drainage trough is corresponding with the through hole of lower cover.It is noted that due to the symmetric design of thread mixing unit, upper cover and lower cover
All can mutually replace use, the flow channel of varying number of can arranging in pairs or groups whereby meets different mode of operation demands.
As shown in Figure 4, mode of operation 1:
Assume that liquid to be mixed is A and B.A and B all pumps into blender from blender upper cover, and wherein P1, P3 and P5 path leads to
Entering A liquid, P2 and P4 path is passed through B liquid.Proportioning is by respective proportioning valve on-off control.Five streams of A and B liquid pass through
Drainage trough and spiral flow path import the central mixing zone of mixed cell.(remaining is flowed out after sufficiently mixing by the Q1 through hole of lower cover
Through hole plug blocks).
As it is shown in figure 5, mode of operation 2:
Assume that liquid to be mixed is A and B.A liquid pumps into blender from blender upper cover, wherein the P1 of upper cover, P2, P3, P4
Path is passed through A liquid, and B liquid pumps into blender from blender lower cover, wherein the P1 of lower cover, and P2, P3, P4 path is passed through B liquid.
Proportioning is by respective proportioning valve on-off control.The center that A and B liquid imports mixed cell by drainage trough and spiral flow path mixes
District.Flowed out by the P5 through hole of upper cover after sufficiently mixing.The P5 passage of lower cover blocks with dead plug.
Claims (3)
1. the passive mixing device for high performance liquid chromatography, it is characterised in that: utilize the branch of liquid stream to converge realization again
The high efficiency mixing of flowing phase and uniformly mixing, including thread mixing unit, the end face of thread mixing unit compresses to seal and installs
Having upper cover, the bottom surface of thread mixing unit compresses and is sealed and installed with lower cover, wherein:
Described thread mixing unit includes thread mixing unit disc, thread mixing unit disc center be provided with as in
The central through hole of heart mixed zone, the end face of thread mixing unit disc, bottom surface be provided with the circular groove as drainage trough the most with one heart,
Thread mixing unit disc end face, bottom surface further respectively have multiple tracks in corresponding surface uniform ring around the spiral channel of central through hole,
Spiral channel is as spiral flow path, and each spiral flow path one end notch is respectively communicated to the drainage trough of correspondence, each spiral flow path
Other end notch is respectively communicated to central mixing zone, and each spiral flow path degree of depth is line from drainage trough to direction, central mixing zone
Property increase；
Described upper cover includes that upper cover disc, upper cover disc end face are coaxially provided with the conical surface, are provided with straight down from conical surface end face
The through conical surface, the central through hole of upper cover disc, the conical surface is provided with liquid input through hole, and multiple liquid input through hole is the most equal
Even being distributed in around conical surface central shaft, and multiple liquid input through hole tilts the through conical surface, upper cover disc, upper cover passes through bottom surface
When compression is sealingly mounted at thread mixing unit end face, the central through hole of upper cover connects with thread mixing unit center mixed zone,
The liquid input aperture, through hole lower end of upper cover is respectively communicated to the drainage trough of thread mixing unit；
Described lower cover includes lower cover disc, is eccentrically provided with through hole in lower cover disc, and described lower cover compression is sealed in spiral and mixes
When closing unit bottom surface, in lower cover, aperture, through hole upper end is communicated to the drainage trough of thread mixing unit.
A kind of passive mixing device for high performance liquid chromatography the most according to claim 1, it is characterised in that: described spiral shell
In rotation mixed cell, the spiral flow path of thread mixing unit disc end face and bottom surface is in being alternately distributed.
A kind of passive mixing device for high performance liquid chromatography the most according to claim 1, it is characterised in that: upper cover and
Lower cover can mutually replace use, meets different mode of operation demands with the flow channel of realization collocation varying number.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610527610.6A CN106166453A (en) | 2016-07-06 | 2016-07-06 | A kind of passive mixing device for high performance liquid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610527610.6A CN106166453A (en) | 2016-07-06 | 2016-07-06 | A kind of passive mixing device for high performance liquid chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106166453A true CN106166453A (en) | 2016-11-30 |
Family
ID=58066159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610527610.6A Withdrawn CN106166453A (en) | 2016-07-06 | 2016-07-06 | A kind of passive mixing device for high performance liquid chromatography |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106166453A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108393008A (en) * | 2018-04-26 | 2018-08-14 | 无锡先导智能装备股份有限公司 | Rabbling mechanism and pulping device |
| US11185830B2 (en) | 2017-09-06 | 2021-11-30 | Waters Technologies Corporation | Fluid mixer |
| US11555805B2 (en) | 2019-08-12 | 2023-01-17 | Waters Technologies Corporation | Mixer for chromatography system |
| US11821882B2 (en) | 2020-09-22 | 2023-11-21 | Waters Technologies Corporation | Continuous flow mixer |
| US11898999B2 (en) | 2020-07-07 | 2024-02-13 | Waters Technologies Corporation | Mixer for liquid chromatography |
-
2016
- 2016-07-06 CN CN201610527610.6A patent/CN106166453A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11185830B2 (en) | 2017-09-06 | 2021-11-30 | Waters Technologies Corporation | Fluid mixer |
| CN108393008A (en) * | 2018-04-26 | 2018-08-14 | 无锡先导智能装备股份有限公司 | Rabbling mechanism and pulping device |
| US11555805B2 (en) | 2019-08-12 | 2023-01-17 | Waters Technologies Corporation | Mixer for chromatography system |
| US11898999B2 (en) | 2020-07-07 | 2024-02-13 | Waters Technologies Corporation | Mixer for liquid chromatography |
| US11821882B2 (en) | 2020-09-22 | 2023-11-21 | Waters Technologies Corporation | Continuous flow mixer |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20161130 |
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| WW01 | Invention patent application withdrawn after publication |