WO2021163958A1

WO2021163958A1 - Mixing device and driving method therefor, and testing assembly

Info

Publication number: WO2021163958A1
Application number: PCT/CN2020/076029
Authority: WO
Inventors: 胡立教; 张玙璠; 崔皓辰; 袁春根; 李鸿全; 李婧; 甘伟琼
Original assignee: 京东方科技集团股份有限公司; 北京京东方健康科技有限公司
Priority date: 2020-02-20
Filing date: 2020-02-20
Publication date: 2021-08-26
Also published as: CN113544489A; CN113544489B

Abstract

Disclosed are a mixing device (100) and a driving method therefor, and a testing assembly. The mixing device (100) is used for operating a testing chip (01) comprising a membrane cavity (02), and the mixing device (100) comprises a testing chip mounting portion (10) and a movable driving portion (20), wherein the testing chip mounting portion (10) is configured for mounting a testing chip (01); and the driving portion (20) is configured to be in contact with the membrane cavity (02) of the mounted testing chip (01), and can repeatedly squeeze different parts of the membrane cavity (02). The mixing device (100) can mix reagents in the single membrane cavity (02) of the testing chip (01), has a simple structure, is easy to implement, can avoid the wasting of the reagents in connecting flow channels between multiple areas during mixing between the multiple areas, is conducive to reducing the loss of the reagents or magnetic beads during mixing, and can shorten the mixing time, improve the mixing uniformity, and improve the level of control during mixing.

Description

Mixing device and its driving method and detection component

Technical field

The embodiments of the present disclosure relate to a hybrid device, a driving method thereof, and a detection component.

Background technique

Microfluidic chip technology integrates the basic operation units of sample preparation, reaction, separation, and detection involved in the fields of biology, chemistry, and medicine into a chip with micrometer-scale microchannels to automatically complete the entire process of reaction and analysis. The chip used in this process is called a microfluidic chip, which can also be called a Lab-on-a-chip (Lab-on-a-chip). Microfluidic chip technology has the advantages of low sample consumption, fast analysis speed, easy to make portable instruments, suitable for instant and on-site analysis, etc., and has been widely used in many fields such as biology, chemistry and medicine.

Summary of the invention

At least one embodiment of the present disclosure provides a mixing device for operating a detection chip including a membrane cavity, wherein the mixing device includes: a detection chip mounting part configured to mount the detection chip; a movable driving part, wherein The driving part is configured to be in contact with the membrane cavity of the mounted detection chip, and can repeatedly squeeze different parts of the membrane cavity.

For example, in the mixing device provided by an embodiment of the present disclosure, the driving part includes a plurality of movable parts, the plurality of movable parts can be in contact with different parts of the membrane cavity, and the plurality of movable parts are configured to Moving alternately to opposite directions along a direction perpendicular to the detection chip to repeatedly squeeze different parts of the membrane cavity.

For example, in the mixing device provided by an embodiment of the present disclosure, the plurality of movable parts includes a column with a fan-shaped cross-sectional shape, and the plurality of movable parts are adjacent to each other and are combined as a whole to form a cylinder; the membrane cavity It is circular, and the cross-sectional diameter of the cylinder is smaller than or equal to the diameter of the membrane cavity.

For example, in the mixing device provided by an embodiment of the present disclosure, the part where the plurality of movable parts are in contact with each other includes a groove, the grooves of the plurality of movable parts are combined to form a cylindrical cavity, and the driving part further includes The magnetic component is arranged in the cylindrical cavity and can move in the cylindrical cavity along the axis direction of the cylindrical cavity.

For example, in the mixing device provided by an embodiment of the present disclosure, the cross-sectional shape of the cylindrical cavity is a circle, and the cross-sectional shape of the magnetic component is a circle, and the cross-sectional diameter of the magnetic component is that of the membrane. 1/3 to 2/3 of the diameter of the cavity.

For example, in the mixing device provided by an embodiment of the present disclosure, the thickness of the part covering the groove on the end surfaces of the plurality of movable parts in contact with the membrane cavity is 0.1 mm-2 mm.

For example, in the mixing device provided by an embodiment of the present disclosure, the plurality of movable parts includes two movable parts, and the cross-sectional shape of the movable parts is semicircular.

For example, the mixing device provided by an embodiment of the present disclosure further includes multiple sets of first power components and multiple first transmission components, wherein the multiple first transmission components are connected to the multiple movable components in a one-to-one correspondence, so The plurality of first transmission components are connected to the plurality of groups of first power components in one-to-one correspondence, and the plurality of groups of first power components are configured to provide power to drive the corresponding plurality of first transmission components, and the plurality of first power components are configured to provide power to drive the corresponding plurality of first transmission components. The first transmission component is configured to transmit the power provided by the plurality of groups of first power components to the corresponding plurality of movable components to drive the corresponding plurality of movable components.

For example, in the mixing device provided by an embodiment of the present disclosure, at least one of the plurality of groups of first power components includes a motor and a cam, the cam is connected to the motor in an eccentric manner, and the plurality of first transmissions At least one of the components includes a connecting rod and a bearing, the inner ring of the bearing is rotatably connected with one end of the connecting rod, the outer ring of the bearing is in contact with the cam, and the other end of the connecting rod is connected to the corresponding movable The parts are fixedly connected.

For example, the mixing device provided by an embodiment of the present disclosure further includes a second power component and a second transmission component, wherein the second transmission component is connected to the magnetic component and the second power component, and the second power component is connected to the magnetic component and the second power component. The component is configured to provide power to drive the second transmission component, and the second transmission component is configured to transmit the power provided by the second power component to the magnetic component to drive the magnetic component.

For example, the mixing device provided by an embodiment of the present disclosure further includes a seal ring and a seal ring drive unit, wherein the seal ring is disposed on the detection chip mounting portion, and the detection chip includes a plurality of strips connected to the membrane cavity. A flow channel and an annular sealing area surrounding the membrane cavity, the annular sealing area overlaps the plurality of flow channels, when the detection chip is mounted on the detection chip mounting portion, the sealing ring is different The parts can be in contact with or separated from the annular sealing area, and the sealing ring is configured to control at least one of the plurality of flow passages to open and control the plurality of flow passages by contacting or separating with the annular sealing area. At least one of the flow channels is closed, and the plurality of flow channels are controlled to be closed, the sealing ring driving unit is configured to drive different parts of the sealing ring to contact or separate from the annular sealing area respectively.

For example, in the mixing device provided by an embodiment of the present disclosure, the seal ring drive unit includes multiple sets of seal ring drive components, and each set of the multiple sets of seal ring drive components includes an arc-shaped contact piece, a third power component, and a third power component. Three transmission components, the arc-shaped contact piece can be in contact with the sealing ring, the third transmission component is connected with the arc-shaped contact piece and the third power component, and the third transmission component is configured to The power provided by the third power component is transmitted to the arc-shaped contact piece, so that the corresponding contact part of the sealing ring and the arc-shaped contact piece contacts or separates from the annular sealing area.

For example, in the mixing device provided by an embodiment of the present disclosure, the multiple sets of seal ring driving parts include two sets of seal ring driving parts, and the part of the seal ring that contacts one of the arc-shaped contacts is 1/2 circle. arc.

For example, in the mixing device provided by an embodiment of the present disclosure, the multiple sets of seal ring drive components are configured to be able to rotate with the central axis of the seal ring as a rotation axis.

For example, in the mixing device provided by an embodiment of the present disclosure, the plurality of flow channels includes three flow channels, and the three flow channels are uniformly distributed along the circumference of the membrane cavity, and the extension of the three flow channels The line intersects at a point, the sealing ring includes a complementary first part and a second part, and the dividing line of the first part and the second part does not overlap the flow channel, and the first part is sealed with the ring When the zone is separated and the second part is in contact with the annular sealing zone, one of the three flow channels is opened, and the other two of the three flow channels are closed. When the second part is in contact with the annular sealing area, the three flow channels are all closed.

For example, in the mixing device provided by an embodiment of the present disclosure, the plurality of flow channels include four flow channels, and the four flow channels are uniformly distributed along the circumference of the membrane cavity, and the extension of the four flow channels The line intersects at a point. The sealing ring includes a complementary first part and a second part. The dividing line of the first part and the second part overlaps with at least one of the flow channels. When the sealing area is separated and the second part is in contact with the annular sealing area, one of the four flow channels is opened, and the other three of the four flow channels are closed. When the second part is in contact with the annular sealing area, the four flow channels are all closed.

At least one embodiment of the present disclosure further provides a detection component, including: the mixing device according to any embodiment of the present disclosure, and the detection chip; wherein, the detection chip includes the membrane cavity, and the mixing device The driving part is configured to be in contact with the membrane cavity and can repeatedly squeeze different parts of the membrane cavity.

At least one embodiment of the present disclosure further provides a method for driving a mixing device according to any one of the embodiments of the present disclosure, including: driving the driving part to repeatedly squeeze the detection chip mounted on the detection chip mounting part Different parts of the membrane cavity.

For example, in the driving method provided by an embodiment of the present disclosure, the driving part includes a plurality of movable parts and a magnetic part, the part where the plurality of movable parts contact each other includes a groove, and the groove of the plurality of movable parts The combination constitutes a cylindrical cavity, and the driving method further includes: moving the magnetic component in the cylindrical cavity along the axial direction of the cylindrical cavity.

For example, in the driving method provided by an embodiment of the present disclosure, the mixing device includes a sealing ring, the detection chip includes an annular sealing area, and the driving method further includes: driving different parts of the sealing ring and the annular sealing area. The sealing area touches or separates.

Description of the drawings

In order to explain the technical solutions of the embodiments of the present disclosure more clearly, the following will briefly introduce the drawings of the embodiments. Obviously, the drawings in the following description only refer to some embodiments of the present disclosure, rather than limiting the present disclosure. .

FIG. 1 is a schematic block diagram of a mixing device provided by at least one embodiment of the present disclosure;

2 is a schematic structural diagram of a mixing device provided by at least one embodiment of the present disclosure;

FIG. 3 is a schematic diagram of the mixing principle of a mixing device provided by at least one embodiment of the present disclosure;

Fig. 4 is a partial structural diagram of the mixing device shown in Fig. 2;

5 is a schematic diagram of a sealing method of the mixing device provided by at least one embodiment of the present disclosure;

6 is a schematic diagram of another sealing method of the mixing device provided by at least one embodiment of the present disclosure;

FIG. 7 is a schematic structural diagram of a sealing ring driving unit of a mixing device provided by at least one embodiment of the present disclosure; and

FIG. 8 is a schematic block diagram of a detection component provided by at least one embodiment of the present disclosure.

Detailed ways

In order to make the objectives, technical solutions, and advantages of the embodiments of the present disclosure clearer, the technical solutions of the embodiments of the present disclosure will be described clearly and completely in conjunction with the accompanying drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, rather than all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by a person of ordinary skill in the art without creative labor are within the protection scope of the present disclosure.

Unless otherwise defined, the technical terms or scientific terms used in the present disclosure shall have the usual meanings understood by those with ordinary skills in the field to which this disclosure belongs. The "first", "second" and similar words used in the present disclosure do not indicate any order, quantity, or importance, but are only used to distinguish different components. Likewise, similar words such as "a", "one" or "the" do not mean a quantity limit, but mean that there is at least one. "Include" or "include" and other similar words mean that the element or item appearing before the word covers the elements or items listed after the word and their equivalents, but does not exclude other elements or items. Similar words such as "connected" or "connected" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "Down", "Left", "Right", etc. are only used to indicate the relative position relationship. When the absolute position of the described object changes, the relative position relationship may also change accordingly.

During the use of the microfluidic chip, the reaction system solution in the microfluidic chip needs to be reacted, such as amplification reaction. The reaction degree and reaction effect of the reaction system solution have a greater impact on the detection result of the microfluidic chip . The reaction system solution is usually formed by mixing multiple reagents, and the mixing effect of the multiple reagents directly affects the reaction degree and reaction effect of the reaction system solution.

In a common microfluidic chip, an open mixing method is often used, that is, multiple reagents are mixed between two areas, which are connected by a flow channel, for example, in two vesicles. Mix it back and forth between, or between the reagent storage tank and other areas connected to it. However, this mixing method will result in waste of reagents and magnetic beads. For example, reagents and magnetic beads will remain in the flow channel between the two mixing areas, resulting in a large amount of reagents and loss of magnetic beads. . Moreover, this mixing method has a longer mixing time and poor mixing uniformity, which will affect the accuracy of the detection result.

At least one embodiment of the present disclosure provides a mixing device, a driving method thereof, and a detection component. The mixing device can mix reagents in a single membrane cavity of a detection chip, has a simple structure, is easy to implement, and can avoid mixing between multiple regions. The waste of reagents connected to the flow channel between multiple areas is beneficial to reduce the loss of reagents or magnetic beads during the mixing process, can shorten the mixing time, improve the uniformity of the mixing, and improve the controllability of the mixing process.

Hereinafter, embodiments of the present disclosure will be explained in detail with reference to the drawings. It should be noted that the same reference numerals in different drawings will be used to refer to the same elements that have been described.

At least one embodiment of the present disclosure provides a mixing device for operating a detection chip including a membrane cavity. The mixing device includes a detection chip mounting part and a movable driving part. The detection chip mounting part is configured to mount the detection chip. The driving part is configured to be in contact with the membrane cavity of the mounted detection chip, and can repeatedly squeeze different parts of the membrane cavity.

Fig. 1 is a schematic block diagram of a mixing device provided by at least one embodiment of the present disclosure. As shown in FIG. 1, the mixing device 100 is used to operate a detection chip 01 including a membrane cavity. The detection chip 01 is, for example, a microfluidic chip and is used, for example, to detect nucleic acid fragments in a sample (such as blood, body fluid, etc.). For example, the membrane cavity is a cavity formed between the elastic membrane of the detection chip 01 and the chip substrate, and can contain liquid.

The hybrid device 100 includes a detection chip mounting part 10 and a movable driving part 20. The detection chip mounting part 10 plays a role of bearing, fixing, etc., and is configured to mount the detection chip 01. The driving part 20 is configured to be in contact with the membrane cavity of the mounted detection chip 01, and can repeatedly squeeze different parts of the membrane cavity. When multiple reagents are delivered to the membrane cavity, the multiple reagents can be uniformly mixed in the membrane cavity by repeatedly squeezing different parts of the membrane cavity. When both the sample and the reagent are delivered to the membrane cavity, by repeatedly squeezing different parts of the membrane cavity, the sample and the reagent can be mixed uniformly, so as to facilitate the extraction, rinsing, elution and other operations of the sample.

Fig. 2 is a schematic structural diagram of a mixing device provided by at least one embodiment of the present disclosure. As shown in FIG. 2, the hybrid device 100 includes a detection chip mounting part 10 and a movable driving part 20.

The detection chip mounting part 10 plays a role of bearing, fixing, etc., and is configured to mount the detection chip 01. For example, the detection chip mounting portion 10 may adopt a structure of a card slot and a clamp, an adhesive, or other applicable structure, as long as the detection chip 01 can be installed, which is not limited in the embodiment of the present disclosure. . For example, FIG. 2 schematically shows that the detection chip mounting portion 10 is a structural member having a gap for accommodating the detection chip 01, but this does not constitute a limitation to the embodiment of the present disclosure.

For example, the detection chip 01 includes a film cavity 02, the film cavity 02 is located on the surface of one side of the detection chip 01 (for example, the lower surface of the detection chip 01 shown in FIG. 2), and the film cavity 02 covers the lower surface of the detection chip 01 A cavity formed between the elastic film and the chip substrate of the detection chip 01. The membrane cavity 02 is used to contain liquid, for example, a sample and a variety of reagents are mixed in the membrane cavity 02 to perform operations such as extraction, rinsing, and elution.

The driving part 20 is configured to be in contact with the membrane cavity 02 of the mounted detection chip 01, and can repeatedly squeeze different parts of the membrane cavity 02. For example, the driving part 20 includes a plurality of movable parts 21. A plurality of movable parts 21 can be in contact with different parts of the membrane cavity 02, and the plurality of movable parts 21 are configured to be able to alternately move in opposite directions along a direction perpendicular to the detection chip 01 to repeatedly squeeze different parts of the membrane cavity 02.

For example, as shown in FIG. 2, the plurality of movable parts 21 includes a first movable part 211 and a second movable part 212. Direction movement. The first direction is, for example, a direction perpendicular to the detection chip 01, and when the detection chip 01 is placed horizontally, the first direction is, for example, a vertical direction. For example, the installation positions of the first movable part 211 and the second movable part 212 correspond to the membrane cavity 02. When the first movable part 211 or the second movable part 212 moves in the direction approaching the detection chip 01 in the first direction, the first movable part 211 or the second movable part 212 can contact the membrane cavity 02 and press the membrane cavity 02. Corresponding parts, thereby deforming the elastic film covering the film cavity 02 and making the space of the film cavity 02 smaller.

For example, the first movable part 211 and the second movable part 212 can alternately move in the opposite direction along the first direction, so that different parts of the membrane cavity 02 can be repeatedly squeezed.

As shown in FIG. 3, when the first movable part 211 moves upward, the second movable part 212 moves downward, so the first movable part 211 can squeeze the corresponding part of the membrane cavity 02 (for example, the membrane cavity shown in FIG. 3). 02), the right part of the membrane cavity 02 will not be squeezed. At this time, the liquid 03 in the membrane cavity 02 is squeezed into the right space of the membrane cavity 02. Similarly, when the first movable part 211 moves downward, the second movable part 212 moves upward, so the second movable part 212 can squeeze the corresponding part of the membrane cavity 02 (for example, the right side of the membrane cavity 02 shown in FIG. 3). Side part), the left part of the membrane cavity 02 will not be squeezed. At this time, the liquid 03 in the membrane cavity 02 will be squeezed into the left space of the membrane cavity 02.

By making the first movable part 211 and the second movable part 212 alternately move in the opposite direction along the first direction, the left and right parts of the membrane cavity 02 can be repeatedly squeezed, so that the liquid 03 in the membrane cavity 02 can be squeezed repeatedly. It flows back and forth in the left space and the right space of the membrane cavity 02, thereby realizing the mixing of the liquid 03. For example, the liquid 03 may contain samples and various reagents, and may contain magnetic beads dispersed therein. For example, the first movable part 211 and the second movable part 212 can also simultaneously squeeze the left and right parts of the membrane cavity 02, so that the liquid 03 in the membrane cavity 02 can be discharged to realize the function of pumping liquid.

Therefore, the mixing device 100 can mix reagents in the single membrane cavity 02 of the detection chip 01, has a simple structure, is easy to implement, and can avoid the waste of reagents connecting flow channels between multiple areas when mixing between multiple areas. It helps to reduce the loss of reagents or magnetic beads during the mixing process. Moreover, this repeated extrusion method can shorten the mixing time, improve the mixing efficiency, and improve the mixing uniformity, so that the liquid 03 can be fully mixed uniformly, and the controllability of the mixing process can be improved.

For example, the volume of the liquid 03 in the membrane cavity 02 is less than or equal to 50% to 90% of the maximum volume of the liquid that the membrane cavity 02 can contain, such as 50% to 80%, such as 60%, so as to achieve better mixing. The effect is to make the liquid 03 mix more evenly.

For example, the plurality of movable parts 21 includes a column with a sector shape in cross section, and the plurality of movable parts 21 are adjacent to each other and are combined as a whole to form a cylindrical body. In FIGS. 2, 3, and 4, in order to show that the plurality of movable parts 21 (for example, the first movable part 211 and the second movable part 212) can alternately move in opposite directions, the plurality of movable parts 21 are represented as The positions in the vertical direction are staggered with each other. It can be understood that when the positions of the plurality of movable parts 21 in the vertical direction are consistent (for example, when the horizontal height is the same), the plurality of movable parts 21 are combined to form a cylinder. For example, as shown in FIG. 4, the membrane cavity 02 is circular, and the cross-sectional diameter of a cylinder formed by the combination of a plurality of movable parts 21 is less than or equal to the diameter of the membrane cavity 02. As a result, the movable member 21 can be brought into contact with the membrane cavity 02 and fully squeezed.

For example, in some examples, as shown in FIGS. 2-4, the plurality of movable parts 21 includes two movable parts, namely, a first movable part 211 and a second movable part 212. At this time, the cross-sectional shape of the movable part 21 is half. Round. It should be noted that, in the embodiment of the present disclosure, the number of movable parts 21 is not limited to two, and can also be any number such as three, four, etc. Correspondingly, the cross-sectional shape of the movable part 21 can be 1/3 circle. Shape, 1/4 circle, etc., which can be determined according to actual requirements, and the embodiments of the present disclosure do not limit this.

For example, as shown in FIGS. 2 and 3, the driving part 20 further includes a magnetic component 22. For example, the part where the plurality of movable parts 21 are in contact with each other includes a groove, and the grooves of the plurality of movable parts 21 are combined to form a cylindrical cavity. For example, the first movable part 211 includes a groove 213, and the second movable part 212 includes a groove 214. The groove 213 and the groove 214 are located at the part where the first movable part 211 and the second movable part 212 are in contact with each other. The groove 213 and The grooves 214 are combined to form a cylindrical cavity (for example, a cylindrical cavity). The magnetic component 22 is disposed in the cylindrical cavity and can move in the cylindrical cavity along the axis direction of the cylindrical cavity. For example, the axial direction of the cylindrical cavity is consistent with the first direction, that is, the magnetic component 22 can move in the first direction, for example, reciprocate in the first direction.

For example, when the cylindrical cavity is a cylindrical cavity, the shape of the magnetic component 22 can be set to a cylinder, so that the magnetic component 22 can move more smoothly in the cylindrical cavity. Of course, the embodiment of the present disclosure is not limited to this, and the shape of the magnetic component 22 can also be a cube or any suitable shape, which can be determined according to actual requirements. For example, the cross-sectional diameter of the magnetic member 22 is smaller than the cross-sectional diameter of the cylindrical cavity. For example, the magnetic component 22 may be any device with magnetism, such as a permanent magnet or an electromagnet, which is not limited in the embodiment of the present disclosure.

For example, the magnetic component 22 is used to adsorb magnetic beads located in the membrane cavity 02. For example, when the detection chip 01 is used, the sample is transported to the membrane cavity 02. Since the surface of the magnetic beads is modified, the nucleic acid fragments in the sample will be adsorbed on the surface of the magnetic beads, thereby binding to the magnetic beads. In the subsequent rinsing process, when the waste liquid needs to be punched out of the membrane cavity 02, the magnetic component 22 needs to generate adsorption force on the magnetic beads, so as to prevent the magnetic beads from flowing out of the membrane cavity 02 with the waste liquid, so that the magnetic beads and their adsorbed The nucleic acid fragment remains in the membrane cavity 02. After the nucleic acid fragments are eluted from the magnetic beads, when the magnetic beads need to be punched out of the membrane cavity 02 along with the waste liquid, the magnetic component 22 needs to prevent the magnetic bead from being adsorbed so that the magnetic beads can flow out of the membrane cavity 02 with the waste liquid. When mixing the liquid in the membrane cavity 02, it is also necessary to prevent the magnetic component 22 from generating an adsorption force on the magnetic beads, so that the magnetic beads can be dispersed in the liquid to facilitate uniform mixing. Since the magnetic component 22 can move in the cylindrical cavity, when the magnetic component 22 is close to the detection chip 01, it can generate adsorption force to the magnetic beads, and when the magnetic component 22 is far away from the detection chip 01, it can not generate adsorption force to the magnetic beads. Therefore, the magnetic member 22 can achieve a desired function.

For example, the cross-sectional shape of the magnetic member 22 is circular, and the cross-sectional diameter of the magnetic member 22 is 1/3 to 2/3 of the diameter of the membrane cavity 02, for example, 1/2. This can ensure that the magnetic component 22 can adsorb all the magnetic beads when it is close to the membrane cavity 02, thereby ensuring that the magnetic beads will not flow out of the membrane cavity 02 with the waste liquid, and will not adsorb the magnetic beads when away from the membrane cavity 02, thereby ensuring that the magnetic beads It can be broken up and mixed uniformly and can flow out of the membrane cavity 02 as required. It should be noted that since the size of the upper surface of the magnetic component 22 determines the magnitude of the magnetic force, the value of the cross-sectional diameter of the magnetic component 22 can be specifically determined according to factors such as the number of magnetic beads, the required adsorption force, and the like. No restrictions.

For example, the thickness of the part covering the groove in the end surface of the plurality of movable parts 21 in contact with the film cavity 02 is 0.1 mm to 2 mm, for example, 1 mm. For example, as shown in FIG. 2, the part covering the groove 213 on the end surface of the first movable part 211 in contact with the film cavity 02 is a thin wall 215, and the thickness of the thin wall 215 is 0.1 mm-2 mm, for example, 1 mm. Correspondingly, the second movable part 212 also has a thin wall (the number is not marked in FIG. 2), and the thickness of the thin wall is also 0.1 mm to 2 mm, for example, 1 mm. In this way, the upper surface of the movable part 21 can be a complete fan-shaped surface, which can completely cover and close to the membrane cavity 02 for sufficient compression, and at the same time, it can ensure that the magnetic weakening of the magnetic part 22 is small, so that The magnetic force generated by the magnetic member 22 can attract the magnetic beads.

For example, as shown in FIG. 2, the hybrid device 100 further includes multiple sets of first power components 30 and multiple first transmission components 40. For example, the plurality of groups of first power components 30 includes two groups, namely the first power component 30a and the first power component 30b; the plurality of first transmission components 40 include two, which are respectively the first transmission component 40a and the first transmission component. 40b.

For example, the plurality of first transmission components 40 are connected to the plurality of movable components 21 in a one-to-one correspondence, and the plurality of first transmission components 40 are connected to the plurality of groups of first power components 30 in a one-to-one correspondence. The plurality of groups of first power components 30 are configured to provide power to drive the corresponding plurality of first transmission components 40, and the plurality of first transmission components 40 are configured to transmit the power provided by the plurality of groups of first power components 30 to the corresponding plurality of activities Component 21 to drive a plurality of corresponding movable components 21.

For example, the first transmission part 40a is connected to the first power part 30a and the first movable part 211, and transmits the power provided by the first power part 30a to the first movable part 211 to drive the first movable part 211 to repeatedly squeeze the film Corresponding part of cavity 02. For example, the first transmission part 40b is connected to the first power part 30b and the second movable part 212, and transmits the power provided by the first power part 30b to the second movable part 212 to drive the second movable part 212 to repeatedly squeeze the film Corresponding part of cavity 02.

It should be noted that in this embodiment, there are two first transmission components 40 and the first power components 30 are two groups, but the embodiment of the present disclosure is not limited to this, the first transmission components 40 and the first power components 30 The number can also be any number, which can be determined according to actual requirements, for example, according to the number of movable parts 21 that need to be driven. For example, the number of the first transmission components 40, the number of the first power components 30, and the number of the movable components 21 are equal to achieve one-to-one correspondence, so that the movable components 21 can be driven to squeeze the film cavity 02.

For example, at least one of the groups of first power components 30 includes a motor and a cam. For example, in some examples, as shown in FIG. 2, the first power component 30a and the first power component 30b each include a motor and a cam. Taking the first power component 30b as an example, the first power component 30b includes a motor 31 and a cam 32, and the cam 32 is connected to the motor 31 in an eccentric manner. The motor 31 is, for example, a stepper motor or other suitable motors. Similarly, the first power component 30a also adopts a similar or identical structure, which will not be repeated here.

For example, at least one of the plurality of first transmission components 40 includes a connecting rod and a bearing. For example, in some examples, as shown in FIG. 2, the first transmission component 40a and the first transmission component 40b each include a connecting rod and a bearing. Taking the first transmission component 40b as an example, the first transmission component 40b includes a connecting rod 41 and a bearing 42. The inner ring of the bearing 42 is rotatably connected with one end of the connecting rod 41, and the outer ring of the bearing 42 is in contact and connected with the cam 32, and the connecting rod 41 The other end is fixedly connected to the corresponding movable part (ie, the second movable part 212). For example, the bearing 42 may be a sliding bearing, a rolling bearing, or other types of bearings. Similarly, the first transmission component 40a also adopts a similar or identical structure, which will not be repeated here.

When the motor 31 drives the cam 32 to rotate, since the cam 32 is connected to the motor 31 in an eccentric manner, the position of the contact part of the cam 32 and the bearing 42 in the vertical direction will change, thereby driving the bearing 42 and the connecting rod 41 to move up and down. Movement, thereby driving the second movable component 212 to move up and down, so as to realize repeated squeezing of the corresponding part of the membrane cavity 02. In this way, the axial movement of the cam 32 is converted into the up and down movement of the second movable part 212 through the connecting rod 41, and the transmission efficiency is high. Similarly, the first movable part 211 is also driven in a similar manner.

For example, by setting the motor speed, the frequency of the up and down movement of the first movable part 211 and the second movable part 212 can be adjusted, thereby adjusting the squeezing frequency of the membrane cavity 02. When the extrusion frequency of the membrane cavity 02 is high, the mixing time of the liquid in the membrane cavity 02 can be shortened, and the mixing efficiency can be improved. When the extrusion frequency of the membrane cavity 02 is low, power consumption can be reduced, and the liquid in the membrane cavity 02 can be prevented from heating up due to extrusion.

For example, as shown in FIG. 2, the mixing device 100 further includes a second power component 50 and a second transmission component 60. The second transmission component 60 is connected to the magnetic component 22 and the second power component 50. The second power component 50 is configured to provide power to drive the second transmission component 60. The second transmission component 60 is configured to transmit the power provided by the second power component 50 to the magnetic component 22 to drive the magnetic component 22. For example, the second power component 50 may include a motor and a cam, the second transmission component 60 may include a connecting rod and a bearing, and the specific structure of the second power component 50 and the second transmission component 60 may be the same as those of the first power component 30 and the first power component 30 and the second power component. A transmission component 40 is similar and will not be repeated here.

When the second power component 50 and the second transmission component 60 drive the magnetic component 22 to move and approach the detection chip 01, the magnetic beads in the membrane cavity 02 can be adsorbed by the magnetic component 22; when the second power component 50 and the second transmission component 60 When the magnetic component 22 is driven to move away from the detection chip 01, the magnetic beads in the membrane cavity 02 will not be attracted by the magnetic component 22. Thus, the control of the magnetic beads can be achieved.

It should be noted that, in the embodiments of the present disclosure, the specific structural forms of the first power component 30, the first transmission component 40, the second power component 50, and the second transmission component 60 are not limited to the structural forms described above, and may also be For other applicable structural forms, such as a hydraulic drive device, a pneumatic drive device, etc., the embodiments of the present disclosure do not limit this.

The following is a brief description of the working mode of each power component.

When the detection chip 01 is used, the solution after the sample is lysed is injected into the membrane cavity 02. At this time, the motor in the first power component 30 is controlled to rotate so that the multiple movable components 21 move downwards to move away from the membrane cavity. 02. After the solution to be lysed enters the membrane cavity 02, the flow channel connected to the membrane cavity 02 is closed (for example, the sealing ring 70 described below can be used for sealing), so that the motor in the first power component 30 rotates at a high speed, thereby driving the The two movable parts 21 alternately and rapidly squeeze the membrane cavity 02 to mix the solution in the membrane cavity 02 and the embedded magnetic beads. Through mixing, nucleic acid fragments are adsorbed on the surface of the magnetic beads.

Then, the motor in the second power component 50 rotates, so that the magnetic component 22 moves upwards to approach the membrane cavity 02 to attract the magnetic beads in the membrane cavity 02. At the same time, open the flow channel connected to the membrane cavity 02 and control the rotation of the motor in the first power component 30 so that the multiple movable parts 21 move upwards to simultaneously squeeze the membrane cavity 02 to discharge the liquid in the membrane cavity 02 .

Then, the impurities are removed by rinsing and elution, leaving the nucleic acid fragments for subsequent amplification. During rinsing and elution operations, when the magnetic beads need to be attracted, the motor in the second power component 50 rotates to move the magnetic component 22 upwards, thereby approaching the membrane cavity 02 to attract the magnetic beads in the membrane cavity 02 . When there is no need to attract the magnetic beads (for example, when the magnetic beads need to be dispersed), the motor in the second power component 50 rotates to move the magnetic component 22 downwards, so as to move away from the membrane cavity 02, so that the magnetic beads are not attracted The role of.

Fig. 5 is a schematic diagram of a sealing method of the mixing device provided by at least one embodiment of the present disclosure. For example, as shown in FIG. 5, the mixing device 100 further includes a sealing ring 70. For example, the sealing ring 70 is provided on the detection chip mounting portion 10, for example, a mounting frame can be provided in the testing chip mounting portion 10, and the sealing ring 70 can be provided on the mounting frame. In the embodiment of the present disclosure, the sealing ring 70 may be disposed on the detection chip mounting portion 10 in any applicable manner, and the embodiment of the present disclosure does not limit this. For example, the sealing ring 70 may be an O-shaped sealing ring, and the material of the sealing ring 70 may be a deformable material such as rubber or resin, which is not limited in the embodiment of the present disclosure.

For example, the detection chip 01 includes a plurality of flow channels 04 communicating with the membrane cavity 02 and an annular sealing area 021 surrounding the membrane cavity 02, and the annular sealing area 021 overlaps the plurality of flow channels 04. When the detection chip 01 is mounted on the detection chip mounting portion 10, different parts of the sealing ring 70 can be in contact with or separated from the annular sealing area 021, respectively. The sealing ring 70 is configured to control at least one of the plurality of flow channels 04 to open and to control at least one of the plurality of flow channels 04 to close by contacting or separating with the annular sealing area 021. For example, the sealing ring 70 may also be configured to control the closure of multiple flow channels 04 by contacting the annular sealing area 021, thereby sealing the membrane cavity 02.

For example, as shown in FIG. 5, in some examples, the plurality of flow passages 04 includes three flow passages, which are a first flow passage 041, a second flow passage 042, and a third flow passage 043, respectively. For example, the first flow channel 041, the second flow channel 042, and the third flow channel 043 are divergently distributed (for example, evenly distributed) along the circumference of the film cavity 02, and the first flow channel 041, the second flow channel 042, and the third flow channel The extension lines of 043 intersect at one point.

For example, the seal ring 70 includes a first portion 71 and a second portion 72 that are complementary, and the dividing line (the dashed line shown in FIG. 5) of the first portion 71 and the second portion 72 does not overlap the flow channel 04. For example, the first part 71 and the second part 72 are half of the sealing ring 70 respectively.

When the first part 71 is separated from the annular sealing area 021 and the second part 72 is in contact with the annular sealing area 021, the second part 72 squeezes the annular sealing area 021 so that the elastic film at the annular sealing area 021 is deformed to closely adhere to the chip substrate , So that the second flow channel 042 and the third flow channel 043 can be closed, and the first flow channel 041 is in an open state, that is, one of the three flow channels 04 (for example, the first flow channel 041) is open, and the three flow channels The other two flow channels in 04 (for example, the second flow channel 042 and the third flow channel 043) are closed. In this way, the functions of liquid feeding and discharging can be realized. For example, the reagent can flow into the membrane cavity 02 through the first flow channel 041, or the waste liquid in the membrane cavity 02 can flow out through the first flow channel 041.

For example, when the mixing device 100 is used for mixing operation, that is, when a plurality of movable parts 21 are alternately squeezed into the film cavity 02, the first part 71 and the second part 72 of the sealing ring 70 can both squeeze the annular sealing area. 021, so that the multiple flow channels 04 are closed to realize the sealing of the membrane cavity 02 and facilitate the mixing of the liquid in the membrane cavity 02. For example, when the first part 71 and the second part 72 are in contact with the annular sealing area 021, the first part 71 and the second part 72 both squeeze the annular sealing area 021, so that the first flow passage 041, the second flow passage 042 and the second All three runners 043 are closed. In the usual open mixing method, the liquid passage connected to the membrane cavity is in an open state, so the specific length of the liquid entering the liquid passage cannot be controlled. Compared with the usual open mixing method, the mixing device 100 provided by the embodiment of the present disclosure can minimize the loss and uncontrollability of reagents during the mixing process.

By setting the sealing ring 70, the sealing of the membrane cavity 02 can be achieved, and the opening and closing of the flow channel 04 can be flexibly controlled. The operation is simple, easy to implement, and can facilitate the realization of the detection chip 01 (such as a microfluidic chip) during use The operation of liquid feeding, draining, mixing and so on. Using the sealing ring 70 as a sealing component can effectively reduce the cost.

Fig. 6 is a schematic diagram of another sealing method of the mixing device provided by at least one embodiment of the present disclosure. For example, in other examples, as shown in FIG. 6, the plurality of flow channels 04 includes four flow channels, which are a fourth flow channel 044, a fifth flow channel 045, a sixth flow channel 046, and a seventh flow channel 047, respectively. For example, the fourth flow channel 044, the fifth flow channel 045, the sixth flow channel 046, and the seventh flow channel 047 are divergently distributed (for example, evenly distributed) along the circumference of the membrane cavity 02, and the fourth flow channel 044, the fifth flow channel The extension lines of the runner 045, the sixth runner 046, and the seventh runner 047 intersect at one point. For example, the sealing ring 70 includes a complementary first part 71 and a second part 72, the dividing line of the first part 71 and the second part 72 (the dashed line shown in FIG. It overlaps with the seventh runner 047). For example, the first part 71 and the second part 72 are half of the sealing ring 70 respectively.

When the first part 71 is separated from the annular sealing area 021 and the second part 72 is in contact with the annular sealing area 021, the second part 72 squeezes the annular sealing area 021 so that the elastic film at the annular sealing area 021 is deformed to closely adhere to the chip substrate , So that the fifth flow channel 045, the sixth flow channel 046 and the seventh flow channel 047 can be closed, and the fourth flow channel 044 is in an open state, that is, one of the four flow channels 04 (for example, the fourth flow channel) Channel 044) is opened, and the other three of the four flow channels 04 (for example, the fifth flow channel 045, the sixth flow channel 046, and the seventh flow channel 047) are closed.

It should be noted that the fifth runner 045 and the seventh runner 047 are located at the junction of the first part 71 and the second part 72. Due to the compression deformation of the sealing ring 70, the sealing ring 70 and the annular sealing area 021 can be enlarged. Therefore, the fifth flow channel 045 and the seventh flow channel 047 can be covered and pressed, so that the fifth flow channel 045 and the seventh flow channel 047 are closed.

For example, when the first part 71 and the second part 72 are in contact with the annular sealing area 021, the first part 71 and the second part 72 both squeeze the annular sealing area 021, so that the fourth flow channel 044, the fifth flow channel 045, Both the sixth runner 046 and the seventh runner 047 are closed.

It should be noted that in the embodiments of the present disclosure, the number and arrangement of the runners 04 are not limited to the situations shown in Figs. 5 and 6, and the number of runners 04 can also be any number such as 5, 6, etc. This can be determined according to actual needs, and the embodiments of the present disclosure do not limit this.

FIG. 7 is a schematic structural diagram of a sealing ring driving unit of a mixing device provided by at least one embodiment of the present disclosure. For example, as shown in FIG. 7, the mixing device 100 further includes a sealing ring driving unit 80, and the sealing ring driving unit 80 is configured to drive different parts of the sealing ring 70 to contact or separate from the annular sealing area 021 respectively. For clarity of presentation, FIG. 7 only shows the seal ring driving unit 80 and the seal ring 70, but does not show other structures of the mixing device 100, which does not constitute a limitation to the embodiment of the present disclosure.

For example, the seal ring drive unit 80 includes multiple sets of seal ring drive components, such as a first set of seal ring drive components 81 and a second set of seal ring drive components 82. Each of the multiple sets of seal ring drive components includes an arc-shaped contact piece, a third power component, and a third transmission component. For example, the first group of sealing ring driving parts 81 includes arc-shaped contacts 811, third power parts 812, and third transmission parts 813, and the second group of sealing ring driving parts 82 includes arc-shaped contacts 821, third power parts 822, and The third transmission component 823. The arc-shaped

contact pieces

811 and 821 can be in contact with the sealing ring 70.

The third transmission part 813 is connected with the arc-shaped contact 811 and the third power part 812, and the third transmission part 813 is configured to transmit the power provided by the third power part 812 to the arc-shaped contact 811, so that the sealing ring 70 is in contact with the arc The corresponding contact part of the shaped contact piece 811 is in contact with or separated from the annular sealing area 021. Similarly, the third transmission part 823 is connected with the arc-shaped contact 821 and the third power part 822, and the third transmission part 823 is configured to transmit the power provided by the third power part 822 to the arc-shaped contact 821, so that the sealing ring The part 70 corresponding to the arc-shaped contact piece 821 is in contact with or separated from the annular sealing area 021.

For example, under the action of driving, the arc-shaped

contact members

811 and 821 can independently move in the first direction, thereby contacting or separating with the sealing ring 70 respectively, so as to realize the aforementioned functions of closing the flow channel and opening the flow channel. For example, in the example shown in FIG. 7, the seal ring drive unit 80 includes two sets of seal ring drive components, so there are two arc-shaped contact pieces (that is, the arc-shaped contact piece 811 and the arc-shaped contact piece 821), and the seal ring 70 The part in contact with an arc-shaped contact piece (the arc-shaped contact piece 811 or the arc-shaped contact piece 821) is approximately a 1/2 arc.

For example, multiple sets of seal ring drive components 81 and 82 are configured to be rotatable about the central axis of the seal ring 70 as a rotation axis, for example, in the rotation direction shown in FIG. 7. For example, multiple sets of seal ring drive components 81, 82 can be arranged on a rotatable support platform, so that the rotation of multiple sets of seal ring drive components 81, 82 can be achieved by controlling the rotation of the support platform. It should be noted that, in the embodiment of the present disclosure, the rotation of multiple sets of seal ring driving components 81 and 82 can be realized by any applicable arrangement, and the embodiment of the present disclosure does not limit this.

For example, the part where the sealing ring 70 contacts the arc-shaped contact piece 811 is the aforementioned first part 71, and the part where the sealing ring 70 contacts the arc-shaped contact piece 821 is the aforementioned second part 72. When the flow channel distribution in the detection chip 01 is as shown in FIG. 5, if it is necessary to open the second flow channel 042, close the first flow channel 041 and the third flow channel 043, the first set of sealing ring driving components 81 And the second group of sealing ring driving components 82 rotate, correspondingly, the first part 71 and the second part 72 are no longer divided as shown in FIG. 5, but also rotate accordingly, so that the first part 71 and the second part The two flow passages 042 overlap, so that the second part 72 overlaps the first flow passage 041 and the third flow passage 043, thereby controlling the second flow passage 042 to open and the first flow passage 041 and the third flow passage 043 to close.

Similarly, when the flow channel distribution in the detection chip 01 is as shown in FIG. 6, the four flow channels 04 can also be controlled by controlling the rotation of the first group of sealing ring driving parts 81 and the second group of sealing ring driving parts 82. Any one of the flow passages is open and the other three flow passages are closed.

It should be noted that in the embodiments of the present disclosure, the

third power components

812 and 822 can be implemented similarly to the first power component 30, and the

third transmission components

813 and 823 can be implemented similarly to the first transmission component 40. For related descriptions, please refer to the above content, which will not be repeated here.

It should be noted that in the embodiment of the present disclosure, the mixing device 100 may also include more components and components, for example, it may also include a limit device, a power supply, a chassis, etc., which may be determined according to actual needs. The implementation of the present disclosure The example does not restrict this. For example, a temperature control unit, an optical detection unit, etc., used to implement Digital Polymerase Chain Reaction (dPCR) can also be integrated into the mixing device 100, thereby improving the integration of the equipment and making the mixing device 100 The detection chip 01 can be operated and dPCR can be performed, thereby realizing the detection of nucleic acid fragments.

At least one embodiment of the present disclosure further provides a detection component, which includes the hybrid device and the detection chip described in any embodiment of the present disclosure. The detection component can mix reagents in a single membrane cavity of the detection chip, has a simple structure, is easy to implement, can avoid the waste of reagents connected to flow channels between multiple areas when mixing between multiple areas, and is beneficial to reduce reagents or magnetic beads Loss in the mixing process can shorten the mixing time, improve the mixing uniformity, and improve the controllability of the mixing process.

FIG. 8 is a schematic block diagram of a detection component provided by at least one embodiment of the present disclosure. As shown in FIG. 8, the detection component 200 includes a mixing device 210 and a detection chip 220. For example, the mixing device 210 may be the aforementioned mixing device 100, and the detection chip 220 may be the aforementioned detection chip 01. For example, the detection chip 220 includes a membrane cavity (such as the aforementioned membrane cavity 02), and the driving part (such as the aforementioned driving part 20) of the mixing device 210 is configured to be in contact with the membrane cavity and can repeatedly squeeze different parts of the membrane cavity. For example, the detection component 200 can perform dPCR to realize the detection of nucleic acid fragments.

When the detection chip 220 is used for detection, the mixing device 210 can mix the liquid in the membrane cavity of the detection chip 220. In some embodiments, the mixing device 210 can also control the opening and closing of multiple flow channels of the detection chip 220. For the detailed description and technical effects of the mixing device 210 and the detection chip 220, please refer to the detailed description of the mixing device 100 and the detection chip 01 above, which will not be repeated here.

At least one embodiment of the present disclosure further provides a driving method of the hybrid device according to any embodiment of the present disclosure. Using this driving method, the mixing device can be driven to mix the reagents in a single membrane cavity of the detection chip, which is easy to implement, and can avoid the waste of reagents connected to flow channels between multiple areas when mixing between multiple areas, which is beneficial to reduce reagents Or the loss of magnetic beads during the mixing process can shorten the mixing time, improve the mixing uniformity, and improve the controllability of the mixing process.

For example, in some examples, the driving method includes: driving the driving part to repeatedly press different parts of the film cavity of the detection chip mounted on the detection chip mounting part. For example, when the mixing device 100 shown in FIG. 2 is used, the detection chip 01 is mounted on the detection chip mounting part 10, and then the plurality of movable parts 21 in the driving part 20 are driven to repeatedly squeeze the film cavity 02 of the detection chip 01 The different parts of the membrane cavity 02 can be mixed evenly.

For example, in some other examples, the driving method further includes: moving the magnetic component in the cylindrical cavity along the axis of the cylindrical cavity. For example, when the mixing device 100 shown in FIG. 2 is used, the detection chip 01 is mounted on the detection chip mounting portion 10, and then the magnetic component 22 is arranged in the cylindrical cavity along the axis of the cylindrical cavity (for example, the first One direction) movement. When the magnetic component 22 is close to the detection chip 01, the magnetic beads in the membrane cavity 02 may be adsorbed, and when the magnetic component 22 is far away from the detection chip 01, the magnetic beads in the membrane cavity 02 may not be adsorbed.

For example, in still other examples, the driving method further includes: driving different parts of the sealing ring to contact or separate from the annular sealing area. For example, when the mixing device 100 shown in FIGS. 2 and 7 is used, the detection chip 01 is mounted on the detection chip mounting portion 10, and then the sealing ring driving unit 80 is used to drive different parts of the sealing ring 70 and the annular sealing area 021 Contact or separation, thereby controlling any one of the flow channels to open, and control the remaining flow channels to close.

It should be noted that, in the embodiment of the present disclosure, the driving method may further include more steps, and the execution order of each step is not limited, which may be determined according to actual requirements. For example, the operation of squeezing the film cavity 02, the operation of driving the magnetic component 22, and the operation of driving the sealing ring 70 can be performed sequentially in any order, or can be performed at the same time, which can be determined according to actual needs. The embodiment of the present disclosure There is no restriction on this.

For detailed description and technical effects of the driving method, reference may be made to the description of the mixing device 100 in the foregoing, which will not be repeated here.

The following points need to be explained:

(1) The drawings of the embodiments of the present disclosure only refer to the structures involved in the embodiments of the present disclosure, and other structures can refer to the usual design.

(2) In the case of no conflict, the embodiments of the present disclosure and the features in the embodiments can be combined with each other to obtain new embodiments.

The above are only specific implementations of the present disclosure, but the protection scope of the present disclosure is not limited thereto, and the protection scope of the present disclosure should be subject to the protection scope of the claims.

Claims

A mixing device for operating a detection chip including a membrane cavity, wherein the mixing device includes:

The detection chip mounting part is configured to install the detection chip;

Movable drive unit,

Wherein, the driving part is configured to be in contact with the membrane cavity of the mounted detection chip, and can repeatedly squeeze different parts of the membrane cavity.
The mixing device according to claim 1, wherein the driving part includes a plurality of movable parts,

The plurality of movable parts may be in contact with different parts of the film cavity, and the plurality of movable parts may be configured to alternately move to opposite directions along a direction perpendicular to the detection chip to repeatedly squeeze the film Different parts of the cavity.
The mixing device according to claim 2, wherein the plurality of movable parts comprise a column with a sector shape in cross section, and the plurality of movable parts are adjacent to each other and are combined as a whole to form a cylindrical body;

The membrane cavity is circular, and the cross-sectional diameter of the cylinder is less than or equal to the diameter of the membrane cavity.
The mixing device according to claim 3, wherein the part where the plurality of movable parts contact each other includes a groove, and the grooves of the plurality of movable parts combine to form a cylindrical cavity,

The driving part further includes a magnetic component disposed in the cylindrical cavity and movable in the cylindrical cavity along the axis direction of the cylindrical cavity.
The mixing device according to claim 4, wherein the cross-sectional shape of the cylindrical cavity is a circle, and the cross-sectional shape of the magnetic component is a circle,

The cross-sectional diameter of the magnetic component is 1/3 to 2/3 of the diameter of the membrane cavity.
The mixing device according to claim 4 or 5, wherein the thickness of the part covering the groove in the end surface of the plurality of movable parts in contact with the membrane cavity is 0.1 mm to 2 mm.
The mixing device according to any one of claims 2-6, wherein the plurality of movable parts includes two movable parts,

The cross-sectional shape of the movable part is semicircular.
The mixing device according to any one of claims 2-7, further comprising multiple sets of first power components and multiple first transmission components,

Wherein, the plurality of first transmission parts are connected with the plurality of movable parts in a one-to-one correspondence, and the plurality of first transmission parts are connected with the plurality of groups of first power parts in a one-to-one correspondence,

The plurality of groups of first power components are configured to provide power to drive the corresponding plurality of first transmission components,

The plurality of first transmission components are configured to transmit power provided by the plurality of groups of first power components to the corresponding plurality of movable components to drive the corresponding plurality of movable components.
The mixing device according to claim 8, wherein at least one of the plurality of groups of first power components includes a motor and a cam, and the cam is connected to the motor in an eccentric manner,

At least one of the plurality of first transmission components includes a connecting rod and a bearing, the inner ring of the bearing is rotatably connected with one end of the connecting rod, the outer ring of the bearing is in contact with the cam, and the connecting rod The other end is fixedly connected with the corresponding movable part.
The mixing device according to any one of claims 4-6, further comprising a second power component and a second transmission component,

Wherein, the second transmission component is connected with the magnetic component and the second power component,

The second power component is configured to provide power to drive the second transmission component,

The second transmission component is configured to transmit the power provided by the second power component to the magnetic component to drive the magnetic component.
The mixing device according to any one of claims 1-10, further comprising a seal ring and a seal ring drive unit, wherein:

The sealing ring is arranged on the detection chip mounting part,

The detection chip includes a plurality of flow channels communicating with the membrane cavity and an annular sealing area surrounding the membrane cavity, and the annular sealing area overlaps the plurality of flow channels,

When the detection chip is mounted on the detection chip mounting portion, different parts of the sealing ring can be in contact with or separated from the annular sealing area, respectively,

The sealing ring is configured to control at least one of the plurality of flow channels to open and control at least one of the plurality of flow channels to close by contacting or separating with the annular sealing area, and to control the Many runners are closed,

The sealing ring driving unit is configured to drive different parts of the sealing ring to contact or separate from the annular sealing area, respectively.
The mixing device according to claim 11, wherein the sealing ring driving unit comprises a plurality of groups of sealing ring driving parts,

Each of the multiple sets of seal ring driving parts includes an arc-shaped contact piece, a third power part, and a third transmission part. The arc-shaped contact piece can be in contact with the seal ring, and the third transmission part is in contact with the arc The shaped contact is connected to the third power component,

The third transmission component is configured to transmit the power provided by the third power component to the arc-shaped contact piece, so that the part of the sealing ring and the arc-shaped contact piece corresponding to contact with the annular sealing area Contact or separation.
The mixing device according to claim 12, wherein the multiple sets of sealing ring driving parts comprise two sets of sealing ring driving parts,

The part of the sealing ring in contact with one of the arc-shaped contacts is a 1/2 arc.
The mixing device according to claim 12 or 13, wherein the multiple sets of seal ring driving parts are configured to be rotatable with the central axis of the seal ring as a rotation axis.
The mixing device according to any one of claims 11-14, wherein the plurality of flow channels comprise three flow channels, and the three flow channels are uniformly distributed along the circumference of the membrane cavity, and the three flow channels The extension lines of intersect at a point,

The sealing ring includes a complementary first part and a second part, and the dividing line of the first part and the second part does not overlap with the flow channel,

In the case where the first part is separated from the annular sealing area and the second part is in contact with the annular sealing area, one of the three flow passages is opened, and the other two of the three flow passages closure,

When both the first part and the second part are in contact with the annular sealing area, the three flow passages are all closed.
The mixing device according to any one of claims 11-14, wherein the plurality of flow channels includes four flow channels, and the four flow channels are uniformly distributed along the circumference of the membrane cavity, and the four flow channels The extension lines of intersect at a point,

The sealing ring includes a complementary first part and a second part, and a dividing line of the first part and the second part overlaps with at least one of the flow channels,

In the case where the first part is separated from the annular sealing area and the second part is in contact with the annular sealing area, one of the four flow channels is opened, and the other three of the four flow channels are closed ,

When both the first part and the second part are in contact with the annular sealing area, the four flow passages are all closed.
A detection component, including:

The mixing device according to any one of claims 1-16, and

The detection chip;

Wherein, the detection chip includes the membrane cavity, and the driving part of the mixing device is configured to be in contact with the membrane cavity and can repeatedly squeeze different parts of the membrane cavity.
A method for driving a hybrid device according to claim 1, comprising:

The driving part is driven to repeatedly press different parts of the film cavity of the detection chip mounted on the detection chip mounting part.
The driving method according to claim 18, wherein the driving part includes a plurality of movable parts and a magnetic part, a portion where the plurality of movable parts contact each other includes a groove, and the grooves of the plurality of movable parts are combined to form Cylindrical cavity,

The driving method further includes:

The magnetic component is moved in the cylindrical cavity along the axial direction of the cylindrical cavity.
The driving method according to claim 18 or 19, wherein the mixing device includes a sealing ring, and the detection chip includes an annular sealing area,

The driving method further includes:

Different parts of the sealing ring are driven to contact or separate from the annular sealing area.