CN106165782A - A kind of livestock fodder additives - Google Patents
A kind of livestock fodder additives Download PDFInfo
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- CN106165782A CN106165782A CN201610584962.5A CN201610584962A CN106165782A CN 106165782 A CN106165782 A CN 106165782A CN 201610584962 A CN201610584962 A CN 201610584962A CN 106165782 A CN106165782 A CN 106165782A
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- fodder additives
- microcapsule
- livestock fodder
- polylactic acid
- livestock
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Abstract
The invention discloses a kind of livestock fodder additives, belong to field of fodder.A kind of livestock fodder additives of the present invention, including microcapsule, described microcapsule is made up of probiotic bacteria embedding, and described microcapsule exterior surface is covered with polylactic acid film, adds trans 2 hexenoic aldehydes of oligosaccharide in described polylactic acid film.A kind of poultry feed of the present invention has reduction Pediococcus pentosaceus drug resistance impact, significantly reduce it to extend influence, reduce the adverse effect that animal health, human health or environment are produced, broiler (grass section chicken) efficiency of feed utilization can be improved simultaneously, improve growth of meat chicken performance, improve the feature of grass section chicken meat production.
Description
Technical field
The present invention relates to a kind of additive, particularly a kind of livestock fodder additives.
Background technology
Adding probiotic bacteria in poultry farming in feedstuff, balanced by regulation and control intestinal microecology, probiotics of rising in value, suppression has
Evil bacterium, produces many positive impacts to growth of animal, not only has the advantage of antibiotic and completely avoid the drawback of antibiotic,
Probiotic bacteria will have broad application prospects as green feed additive.Therefore meaning great to probiotic products and functional development thereof
Justice.Growth of animal performance is affected by h and E factor.Between Guo Qujishinian, research worker is selected by heredity
Growth of meat chicken performance and efficiency of feed utilization have made great progress.Studies have found that, kind is different, Intestine of Broiler physiology
Function and Bacterial community are the most variant.In the environmental factors affecting growth of meat chicken, most important feed factor also can affect individual
Body intestinal microbial population forms.These researchs disclose growth of meat chicken performance and are associated with its intestinal microflora.Pentose sheet ball
Bacterium can improve the utilization rate of broiler fodder, improves growth performance, improves broiler meat production, but due to its antibiotic-resistant Fourth Ring
Element, may spread the most resistance to tetracycline of microorganism causing other, so not can determine that Pediococcus pentosaceus will not be to animal health, people
Class is healthy or environment has a negative impact, and therefore European Union's refusal approval Pediococcus pentosaceus uses as feed additive.
Summary of the invention
The goal of the invention of the present invention is: for the problem of above-mentioned existence, it is provided that a kind of reduction Pediococcus pentosaceus drug resistance
Impact, significantly reduces it and extends influence, and reduces the adverse effect producing animal health, human health or environment, simultaneously can
Improve broiler (grass section chicken) efficiency of feed utilization, improve growth of meat chicken performance, improve the feed additive of grass section chicken meat production.
The technical solution used in the present invention is as follows:
A kind of livestock fodder additives of the present invention, including microcapsule, described microcapsule is made up of probiotic bacteria embedding, described
Microcapsule exterior surface is covered with polylactic acid film, adds oligosaccharide-trans-2-hexenoic aldehyde in described polylactic acid film.
Owing to have employed technique scheme, polylactic acid film cladding microcapsule after can in Intestine of Broiler slow release, prolong
The long probiotic bacteria time-to-live in gastric juice, increase the action time of probiotic bacteria.Trans-2-hexenoic aldehyde is liquid at normal temperatures,
And there is volatility, abnormal smells from the patient is strong, but is bonded with oligosaccharide molecular so that trans-2-hexenoic aldehyde volatility is substantially reduced, with
Time, by increasing capacitance it is possible to increase small intestinal villous height and height of naps and the ratio of Crypt depth and the increase of height of naps can increase absorption
Area so that meat production is correspondingly improved.
A kind of livestock fodder additives as claimed in claim 1, it is characterised in that: described oligosaccharide-trans-2-hexenoic aldehyde
It is 27:80 with the mass ratio of polylactic acid.
Owing to have employed technique scheme, adding proportion is for be the most preferably worth, and after processing 1h in simulated gastric fluid, survival rate is
87.2%, after processing 3h, survival rate is 74.0%, and after processing 5h, survival rate is 60.4%；At room temperature store the survival rate of 7d
Being 82%, after storage 60d, survival rate is 63%.
A kind of livestock fodder additives of the present invention, described probiotic bacteria is by the mesentery that mass ratio is 1:0.4 bright string coccus
Form with Pediococcus pentosaceus.
Owing to have employed technique scheme, reduce under the cooperation of mesentery bright string coccus and use making of Pediococcus pentosaceus
By risk, meanwhile, there is preferable meat production.At experimental group chicken group 42 age in days meat productivity, (chest muscle rate is 35.41%, lower limb flesh
Rate 46.07%) apparently higher than matched group chicken group (chest muscle rate is 35.64%, lower limb flesh rate 35.00%), volatility wind in chicken breast
Taste material such as (anti-, anti-)-2,4-nonadienal, (instead)-2-hexenoic aldehyde, (instead) 2-octenal and (instead)-2-nonenyl aldehyde etc. contain
Amount is all higher than the chicken breast sample of matched group, and these materials all show fat aroma；It addition, have (instead) of " chicken flavor "-
2-decenal only detects in test group chicken breast sample.
A kind of livestock fodder additives of the present invention, comprising mass fraction in described microcapsule is 0.03%～0.14%
Biological selenium, the sodium alginate of 1%～4%, the calcium chloride of 1%～4%, the defatted milk powder of 3%～7%, the lactose of 3%～7%.
Owing to have employed upper technical scheme, microcapsule adds biological selenium and can make its thymus and spleen t-cell IL-2
Inductive activity significantly improves, and impact and probiotic bacteria on other interleukin levels of blood improve blood IL-1 and IL-2 level
Also improve, it is possible to strengthen the immunity of broiler, reduce the risk using Pediococcus pentosaceus to be brought, it is to avoid its diffusion
Cause other the most resistance to tetracycline of microorganism.The microcapsule made by sodium alginate, calcium chloride, defatted milk powder and lactose, bag
Burying effective, artificial resistance of reverse is preferable, and heat resistanceheat resistant, resistance to compression and anti-gastric acid ability strengthen；Its storage life extends, and is more conducive to store and raw
Produce application.
A kind of livestock fodder additives of the present invention, comprises the biological selenium that mass fraction is 0.07% in described microcapsule,
The sodium alginate of 2%, the calcium chloride of 1%, the defatted milk powder of 5%, the lactose of 6%.
Owing to have employed technique scheme, aforementioned proportion is optimum, and the embedding productivity of microcapsule is the highest, reaches
80.44%.
A kind of livestock fodder additives of the present invention, described biological selenium by comprising the following steps preparation,
Step one, configures culture medium, and including glucose 5.0g, casein peptone 10.0g, beef extractum 10.0g, yeast carries
Take liquid 5.0g, Rhizoma Solani tuber osi 50g, ascorbic acid 0.5g, sodium acetate 5.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, citric acid three
Ammonium 3.7g, Magnesium sulfate heptahydrate 0.2g, manganese sulfate 0.05g, calcium carbonate 20.0g, agar 20g, pH are natural, are settled to 1L, in 115
DEG C sterilizing 30min；
Step 2, adds sodium selenite in culture medium, and selenium concentration is 80 μ g/mL, saccharomyces cerevisiae and candida mycoderma is pressed
1:1 combined inoculation in culture medium, 30 DEG C, in 140r/min rotary shaker, shaken cultivation 12h；
Step 3, after fermentation liquid ultrasonication, loads in bag filter, tightens upper end, dialyse in distilled water, distilled water
The cysteine solution 1mL of middle addition 150g/L, and constantly instill methylene blue solution, until methylene blue is colour-fast, prepare
Concentrate after biological selenium extracting solution.
A kind of livestock fodder additives of the present invention, described probiotic microcapsule is Homogeneous Circular granule, described probiotic bacteria
A diameter of the 0.6 of microcapsule～1.5mm, the thickness of described polylactic acid film is 20～70 μm.
Owing to have employed technique scheme, microcapsule can be promoted animal immune organ by the good absorption of broiler
Growing, as the antigenic storehouse that animal is lifelong, stimulating immune system produces immunne response, and enhancing human body immunity system makes it to cause of disease
Microorganism keeps certain immunity.
A kind of livestock fodder additives of the present invention, described livestock fodder additives is used for improving grass section chicken meat production.
Owing to have employed technique scheme, at experimental group chicken group 42 age in days meat productivity, (chest muscle rate is 35.41%, lower limb flesh
Rate 46.07%) apparently higher than matched group chicken group (chest muscle rate is 35.64%, lower limb flesh rate 35.00%).
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:
1, reduce the impact of Pediococcus pentosaceus drug resistance, significantly reduce it and extend influence, reduce animal health, human health
Or the adverse effect that environment produces, broiler (grass section chicken) efficiency of feed utilization can be improved simultaneously, improve growth of meat chicken performance, change
Shan Cao section chicken meat production.
2, extend the probiotic bacteria time-to-live in gastric juice, increase the action time of probiotic bacteria so that it is thymus and splenic T are thin
Born of the same parents' IL-2 inductive activity significantly improves, the impact of other interleukin levels of blood and probiotic bacteria are improved blood IL-1 and
IL-2 level also improves, it is possible to strengthen the immunity of broiler, reduces the risk using Pediococcus pentosaceus to be brought, keeps away
Exempt from its diffusion and cause other the most resistance to tetracycline of microorganism
Detailed description of the invention
The present invention is described in detail below.
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, to the present invention
It is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
Embodiment 1
By bright to cultured Pediococcus pentosaceus and mesentery string coccus bacterium solution according to centrifugal concentrating after 0.4:1 mix homogeneously,
It is proportionally added into biological selenium, sodium alginate, calcium chloride, defatted milk powder and lactose, after being coated, solidifying, low through 350r/min
The centrifugal 10min of speed collects microcapsule granule, takes out a small amount of sample determination embedding productivity after washing 3 times；Remaining product is placed in-20 DEG C
Pre-cooling 10min ,-80 DEG C of freeze overnight, then carry out 24h lyophilization process.Product is loaded on sealing bag, and room temperature preserves.Biological
The concrete proportioning of selenium, sodium alginate, calcium chloride, defatted milk powder and lactose is shown in Table 1, and preparing probiotic microcapsule is Homogeneous Circular
Grain, a diameter of the 0.6 of probiotic microcapsule～1.5mm.
Weighing a certain amount of particles of polylactic acid and add in dichloromethane organic solution, at 25 DEG C, stirring is allowed to be completely dissolved,
Stir speed (S.S.) is 300r/min, makes film base soln, by oligosaccharide-trans-2-hexenoic aldehyde of preparing in mortar levigate after
In being added to polylactic acid membrane basal liquid, putting into supersonic oscillations 30min, treat that the two is sufficiently mixed, solution is without precipitation granule.
By the evenly laid out placement of probiotic microcapsule prepared in advance on a glass, by the tape casting by above-mentioned configuration
Mixed solution is poured in glass plate, and vibrate in ultrasound wave 10min, and in 26 DEG C of baking ovens, dried taking-up takes off film, and polylactic acid is thin
Film is at probiotic microcapsule surface uniform fold, and thickness is 20～70 μm.
The microcapsule embedded composition proportion of table 1 (mass fraction %)
| Numbering | Biological selenium | Sodium alginate | Calcium chloride | Defatted milk powder | Lactose | Embedding productivity |
| 1 | 0.03 | 1 | 1 | 3 | 3 | 74.31 |
| 2 | 0.14 | 4 | 4 | 7 | 7 | 76.20 |
| 3 | 0.10 | 3 | 2 | 4 | 3 | 75.13 |
| 4 | 0.05 | 2 | 1 | 4 | 5 | 70.26 |
| 5 | 0.06 | 1 | 2 | 7 | 7 | 69.24 |
| 6 | 0.03 | 1 | 2 | 6 | 3 | 79.53 |
| 7 | 0.08 | 3 | 1 | 5 | 4 | 80.04 |
| 8 | 0.12 | 4 | 1 | 5 | 3 | 78.13 |
| 9 | 0.10 | 4 | 3 | 4 | 7 | 62.84 |
| 10 | 0.07 | 2 | 1 | 5 | 6 | 80.44 |
| 11 | 0.07 | 3 | 4 | 6 | 6 | 67.30 |
| 12 | 0.05 | 3 | 4 | 6 | 3 | 74.15 |
| 13 | 0.09 | 2 | 3 | 7 | 5 | 70.93 |
Embodiment 2
Biological selenium by comprising the following steps preparation,
Step one, configures culture medium, and including glucose 5.0g, casein peptone 10.0g, beef extractum 10.0g, yeast carries
Take liquid 5.0g, Rhizoma Solani tuber osi 50g, ascorbic acid 0.5g, sodium acetate 5.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, citric acid three
Ammonium 3.7g, Magnesium sulfate heptahydrate 0.2g, manganese sulfate 0.05g, calcium carbonate 20.0g, agar 20g, pH are natural, are settled to 1L, in 115 DEG C
Sterilizing 30min；
Step 2, adds sodium selenite in culture medium, and selenium concentration is 80 μ g/mL, saccharomyces cerevisiae and candida mycoderma is pressed
1:1 combined inoculation in culture medium, 30 DEG C, in 140r/min rotary shaker, shaken cultivation 12h；
Step 3, after fermentation liquid ultrasonication, loads in bag filter, tightens upper end, dialyse in distilled water, distilled water
The cysteine solution 1mL of middle addition 150g/L, and constantly instill methylene blue solution, until methylene blue is colour-fast, prepare
Concentrate after biological selenium extracting solution.
Embodiment 3
Oligosaccharide-trans-2-hexenoic aldehyde is prepared by the following method: weigh oligosaccharide and trans-2-hexenoic aldehyde in proportion, by widow
Sugared and trans-2-hexenoic aldehyde is dissolved separately in acetone, is added in the there-necked flask with thermometer by oligosaccharide-acetone soln, adds
Enter appropriate DMAP, under conditions of being heated to reflux, be slowly added dropwise trans-2-hexenoic aldehyde, after heating reflux reaction 20h, natural
Cooling, adds such as the distilled water of excess, after product fully precipitates, uses ethyl alcohol recrystallization, productivity 82.1% after precipitation is collected by filtration.
Embodiment 4
Experimental animal: 150 AA broiler hatching egg, purchased from the agriculture limited company of Sichuan jade hat.Hatching process is in Sichuan
Agriculture university poultry breeding produces field to be carried out, eventually for the health of feeding experiment and close 1 aa broiler chicken of body weight totally 120
Only.
Choose health and close 1 aa broiler chicken totally 120 of body weight.Randomly draw 60 chickling (every as feeding group
20 is 1 repetition, totally 3 repetitions in group), (every 20 is 1 repetition to remaining 60 chickling, organizes interior 3 weights as a control group
Multiple).Control group fed basal diet, feeds group and adds in 1g/kg embodiment 1 the 7th group in basal diet in the way of spice
Additive.Carry out the immunity of horse Garrick neck injection and the immunity of infectious bronchitis eye drip during 1 age in days, during 12 age in days, carry out H5+
H9 bird flu and newcastle injecting immune, the immunity of fabricius bursa drip and a puncture kind fowlpox.Matched group, feed group feeding environment, pipe
Reason mode is carried out according to Sichuan Agricultural University's poultry breeding field custom requirements, 6 weeks experimental periods.
At experimental group chicken group 42 age in days meat productivity (chest muscle rate is 35.41%, lower limb flesh rate 46.07%) apparently higher than matched group
Chicken group (chest muscle rate is 35.64%, lower limb flesh rate 35.00%), volatile flavor substance such as (anti-, anti-)-2,4-nonyl two in chicken breast
Olefine aldehydr, (instead)-2-hexenoic aldehyde, (instead) 2-octenal and (instead)-2-nonenyl aldehyde equal size are all higher than the chicken breast sample of matched group
Product, these materials all show fat aroma；It addition, there is (the instead)-2-decenal of " chicken flavor " only at test group chicken breast
Sample detects.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any amendment, equivalent and the improvement etc. made within god and principle, should be included within the scope of the present invention.
Claims (8)
1. a livestock fodder additives, it is characterised in that: including microcapsule, described microcapsule is made up of probiotic bacteria embedding, institute
State microcapsule exterior surface and be covered with polylactic acid film, in described polylactic acid film, add oligosaccharide-trans-2-hexenoic aldehyde.
2. livestock fodder additives as claimed in claim 1 a kind of, it is characterised in that: described oligosaccharide-trans-2-hexenoic aldehyde with
The mass ratio of polylactic acid is 27:80.
3. a kind of livestock fodder additives as claimed in claim 1 or 2, it is characterised in that: described probiotic bacteria by mass ratio is
The mesentery bright string coccus of 1:0.4 and Pediococcus pentosaceus composition.
4. a kind of livestock fodder additives as claimed in claim 3, it is characterised in that: described microcapsule comprises mass fraction
It is the biological selenium of 0.03% ~ 0.14%, the sodium alginate of 1% ~ 4%, the calcium chloride of 1% ~ 4%, the defatted milk powder of 3% ~ 7%, the breast of 3% ~ 7%
Sugar.
5. a kind of livestock fodder additives as claimed in claim 4, it is characterised in that: described microcapsule comprises mass fraction
It is the biological selenium of 0.07%, the sodium alginate of 2%, the calcium chloride of 1%, the defatted milk powder of 5%, the lactose of 6%.
6. a kind of livestock fodder additives as described in claim 4 or 5, its feature spy is: described biological selenium is by including
Prepared by following steps,
Step one, configures culture medium, including glucose 5.0g, casein peptone 10.0g, beef extractum 10.0g, yeast extract
5.0g, Rhizoma Solani tuber osi 50g, ascorbic acid 0.5g, sodium acetate 5.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, Triammonium citrate
3.7g, Magnesium sulfate heptahydrate 0.2g, manganese sulfate 0.05g, calcium carbonate 20.0g, agar 20g, pH are natural, are settled to 1L, go out in 115 DEG C
Bacterium 30min；
Step 2, adds sodium selenite in culture medium, and selenium concentration is 80 μ g/mL, saccharomyces cerevisiae and candida mycoderma is mixed by 1:1
Conjunction is inoculated in culture medium, 30 DEG C, in 140r/min rotary shaker, shaken cultivation 12h；
Step 3, after fermentation liquid ultrasonication, loads in bag filter, tightens upper end, dialyse, add in distilled water in distilled water
Enter the cysteine solution 1mL of 150g/L, and constantly instill methylene blue solution, until methylene blue is colour-fast, prepare biology
Concentrate after selenium extracting solution.
7. a kind of livestock fodder additives as described in claim 1 or 2 or 6, it is characterised in that: described probiotic microcapsule
In Homogeneous Circular granule, a diameter of 0.6 ~ 1.5mm of described probiotic microcapsule, the thickness of described polylactic acid film is 20 ~ 70 μ
m。
8. a kind of livestock fodder additives as claimed in claim 7, it is characterised in that: described livestock fodder additives is used for changing
Shan Cao section chicken meat production.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107594136A (en) * | 2017-09-04 | 2018-01-19 | 安徽秀安生态农业有限公司 | A kind of sorbefacient doll's fish meal |
| CN107668389A (en) * | 2017-09-04 | 2018-02-09 | 安徽秀安生态农业有限公司 | A kind of doll's fish meal |
| CN113388551A (en) * | 2021-07-16 | 2021-09-14 | 新希望六和股份有限公司 | Pediococcus pentosaceus NHB-PpA9601 and application thereof |
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| CN107594136A (en) * | 2017-09-04 | 2018-01-19 | 安徽秀安生态农业有限公司 | A kind of sorbefacient doll's fish meal |
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