CN106137811B - Plant extract composition and application thereof - Google Patents
Plant extract composition and application thereof Download PDFInfo
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Abstract
The invention discloses a plant extract composition and application thereof. The traditional Chinese medicine composition comprises a limonium japonicum extract and a gentian extract, wherein the mass ratio of the limonium japonicum extract to the gentian extract is (1:99) - (99: 1); the limonium sinense kuntze extract is prepared by the following preparation method: dissolving the crude extract of herba Limonii Japonici in water, loading into resin packed column, eluting with water and ethanol solution in sequence, collecting eluates, respectively, and removing ethanol and water under reduced pressure; the gentian extract is prepared by the following preparation method: dissolving the rough extract of radix Gentianae in water, loading into resin packed column, eluting with water and ethanol solution sequentially, collecting eluates, and removing ethanol and water under reduced pressure. The preparation method of the plant extract composition is simple, and the prepared plant extract composition can be used as a tyrosinase inhibitor and an antioxidant active agent; further preferably in the preparation of skin care or cosmetic products.
Description
Technical Field
The invention relates to the field of plant extraction, and particularly relates to a plant extract composition and application thereof.
Background
In 1956 Harman proposed the theory of free radical aging [ Harman, D.aging: A the organic based on free and radial chemistry.J.Gerontol.1956,11:289- "300 ], which considers that too many free radicals are a significant cause of biological aging. According to the theory, excessive active oxygen free radicals in the body react with unsaturated fatty acid to generate substances such as malonaldehyde, and the like, and the malonaldehyde reacts with proteins on cell membranes to generate brown pigment which is deposited on the skin to form various color spots. The excessive free radicals can also cause the collagen fibers and the elastic fibers in the skin to be crosslinked and denatured, become brittle and lose elasticity, and when the skin moisture is insufficient, the elastic fibers are easy to break, and skin aging phenomena such as dark lines, fine lines, wrinkles and the like occur. In addition, ionic radiation in the environment and environmental pollutants such as air pollution and chemicals also cause the continuous production of free radicals in living organisms. For example, the ultraviolet radiation in the environment can stimulate fibroblasts and mitochondria in the dermis of the skin, releasing superoxide anions, which are converted to other more destructive free radicals in excess. Although the human body has an antioxidant defense system capable of maintaining a balance between oxidation and oxidation to slow down the generation of active oxygen and free radicals, if the human body is excessively exposed to the sun for a long time, the large amount of free radicals generated in the human body may cause the antioxidant defense ability of the skin to be lowered, resulting in skin damage, such as photoaging, epidermal wrinkles, skin immunity disorder, etc. The antioxidant is a substance having the ability to capture free radicals, and can scavenge active free radicals in the skin, relieve oxidative stress damage of the skin caused by free radicals, and improve skin aging caused by oxidative stress. Free radical scavenging antioxidants in vivo, such as vitamin E and vitamin C, are known, and plant-derived antioxidants such as lotus flower and plum fruit extracts have been reported (patent document CN201110158540.9 discloses a composition for external skin application containing lotus flower extract and plum fruit extract as active ingredients and having antioxidant activity).
Regarding the formation of melanin in the skin, studies have been conducted to show that it is mainly caused by biochemical reactions in melanocytes, i.e., tyrosine generates dopaquinone under the action of tyrosinase as a catalyst, and then melanin is formed by enzymatic catalysis or non-enzymatic oxidation. As for the whitening active substance for inhibiting the production of melanin, inhibition of tyrosinase activity is generally a major target. Tyrosinase inhibitors derived from plant extracts are used as potential whitening active substances, and have expectations of safety and low skin irritation, and various developed reports have been made so far, such as a method for detecting the whitening effect of an ampelopsis japonica extract in patent document CN 201310411195.4; application of CN201310382406.6 aquilaria sinensis pericarp extract in preparing whitening cosmetics.
The herb of Buxue Cao is also called lover's herb, Yizhimei, Haoyao, Baihuayuqian Xiang, and Zhonghua Buxue Cao (atlas of higher plants in China). The herba Limonii Japonici contains tannin, flavone, and anthocyanidin, such as myricitrin, rutin, myricetin, quercetin, and tetrahydroxyflavone. The main pharmacological actions of the blood-nourishing grass are inflammation diminishing, pain relieving, blood circulation promoting, blood enriching, menstruation regulating, bleeding stopping and the like, and the whole grass is collected for fresh use or dry use when the flowers are just bloomed. The Chinese plant record states that the blood-tonifying herbs are traditional national herbs of Kazak, and the roots or whole herbs of the blood-tonifying herbs are used for stopping bleeding, astringing and inducing diuresis in folk. Xinjiang plant will record Chinese herbal medicine and plant lovers in flowerpots as flowers. The blood-nourishing grass is an important flower-matching material because of small flowers, dry film, elegant color and long viewing period.
Limonium sinense (Girard) Kuntze is flower or whole plant of Limonium millefolium of Plumbaginaceae. The herb of Buxue Cao is also called lover's herb, Yizhimei, Haoyao, Baihuayuqian Xiang, and Zhonghua Buxue Cao (atlas of higher plants in China). The herba Limonii Japonici contains tannin, flavone, and anthocyanidin, such as myricitrin, rutin, myricetin, quercetin, and tetrahydroxyflavone. The main pharmacological actions of the blood-nourishing grass are inflammation diminishing, pain relieving, blood circulation promoting, blood enriching, menstruation regulating, bleeding stopping and the like, and the whole grass is collected for fresh use or dry use when the flowers are just bloomed. The Chinese plant record states that the blood-tonifying herbs are traditional national herbs of Kazak, and the roots or whole herbs of the blood-tonifying herbs are used for stopping bleeding, astringing and inducing diuresis in folk. Xinjiang plant will record Chinese herbal medicine and plant lovers in flowerpots as flowers. The blood-nourishing grass is an important flower-matching material because of small flowers, dry film, elegant color and long viewing period. The Limonium has high plants, long flowering branches and can not wither for a long time, and the Limonium has purple, light purple, rose powder, blue, red, white and yellow varieties which are called as 'forget-me-not', the market of 'forget-me-not' fresh flowers, dried flowers and scented tea is not flowers of the plant forget-me-not (Myosotis silvatica ehrh. ex Hoffm.) of the genus Myosotis in the family Boraginaceae, but actually is flowers of the Limonium sinense (Girard) Kuntze or the Limonium bicolor (Bag.) Kuntze.
Gentiana (gentian) L) plants are distributed in mainland china, russia, japan, korea, etc., and are grown in regions at an altitude of 400 m to 1,700 m. Chinese gentian belongs to more than 240 species, is mostly produced in southwest alpine regions, has only a few species around Beijing, and is called three-mountain flowers in the world together with rhododendron and primula. Gentian is also an important medicinal plant, and roots and rhizomes of gentian have the effects of clearing heat, purging liver and arresting convulsion. Radix Gentianae contains compounds such as gentiopicroside, gentisic and triterpenes.
Disclosure of Invention
The invention aims to solve the technical problem of providing a plant extract composition and application thereof. The preparation method of the plant extract composition is simple, and the prepared plant extract composition can be used as a tyrosinase inhibitor and an antioxidant active agent; further preferably in the preparation of skin care or cosmetic products.
The invention provides a plant extract composition, which comprises a limonium japonicum extract and a gentian extract, wherein the mass ratio of the limonium japonicum extract to the gentian extract is (1:99) - (99: 1);
the limonium sinense kuntze extract is prepared by the following preparation method: (1) mixing the limonium sinense kuntze with a solvent, extracting, filtering, and removing the solvent under reduced pressure to obtain a crude extract of the limonium sinense kuntze; (2) dissolving the crude extract of herba Limonii Japonici in water, loading into resin packed column, eluting with water and ethanol solution in sequence, collecting eluates, respectively, and removing ethanol and water under reduced pressure;
the gentian extract is prepared by the following preparation method: (1) mixing radix Gentianae with solvent, extracting, filtering, and removing solvent under reduced pressure to obtain radix Gentianae crude extract; (2) dissolving the rough extract of radix Gentianae in water, loading into resin packed column, eluting with water and ethanol solution sequentially, collecting eluates, and removing ethanol and water under reduced pressure.
The mass ratio of the limonium japonicum extract to the gentian extract is preferably (1:10) - (10:1), more preferably (1:1) - (10:1), and most preferably 1: 1.
In the preparation method of the limonium sinense kuntze extract, the ethanol solution elution is preferably performed by sequentially performing concentration gradient elution with 10-30% ethanol water solution, 31-50% ethanol water solution, 51-70% ethanol water solution, 71-90% ethanol water solution and 91-99% ethanol water solution, and more preferably by sequentially performing concentration gradient elution with 20% ethanol water solution, 40% ethanol water solution, 60% ethanol water solution, 80% ethanol water solution and 95% ethanol water solution; the percentages are volume percentages.
Wherein, the extract of the limonium sinense kuntze is preferably the extract obtained by eluting with 31 percent to 50 percent of ethanol water solution; more preferably 40% ethanol aqueous solution; the percentages are volume percentages.
In the preparation method of the gentian extract, the ethanol solution is preferably subjected to concentration gradient elution sequentially by using a 10-50% ethanol water solution, a 51-90% ethanol water solution and a 91-99% ethanol water solution, and more preferably by using a 30% ethanol water solution, a 70% ethanol water solution and a 95% ethanol water solution; the percentages are volume percentages.
Wherein, the gentian extract is preferably an extract obtained by eluting with 51 to 90 percent of ethanol water solution; more preferably 70% ethanol aqueous solution; the percentages are volume percentages.
Wherein the Limonium sinense (Girard) Kuntze, Limonium bicolor (Bag.) Kuntze, Limonium karst Kaschgalium (Rupr.) Ik. -Gal.), Limonium giganteum (Willd.) Kuntze, Limonium paniculatum (Limonium drechnum Ik. -Gal.), Limonium flexuosum (L.) Kuntze, Limonium specimen (Ligum flavum) Kuntze, Limonium sanguinum sanguinicum (L.) Kuntze), Limonium sanguinea (Ligusticum japonicum) Kuntze, Limonium nodosum (Ligusticum Ik. -Gal.), Limonium sanguinea (Ligustium sanguinea), Limonium sanguinea (Ligusticum japonicum) Kuntze, Limonitum (Ligusticum japonicum) Hayata, Limonitum (Kimura) Kuntze, Limonium sanguinium sanguinea, Limonitum (Ligusticum japonicum) Kuntze, Limonitum (Ligusticum japonicum), Limonitum (Ligusticum japonicum (L.) Kuntze), Limonitum (Ligusticum japonicum (Hawth) Kuntze), Limonitum (Ligusticum japonicum (Ligusticum Ik. -Gal), Limonitum (Ligusticum japonicum), Limonitum (Ligusticum) Kuntze, Limonitum (Ligusticum japonicum, Limonitum (Ligusticum) Kuntze, Limonitum), Limonitum (Ligusticum japonicum (Ligusticum) Hawth (Ligusticum, One or more of Limonitum tenellum (Thurcz.) Kuntze, Limonitum otolepis (Schrenk) Ktze, and Limonitum aureum (L.) Hill), preferably Limonitum sinense (Girard) Kuntze.
In the preparation method of the limonium japonicum extract, the solvent is preferably an ethanol water solution with the volume percentage of 60-80%.
Wherein the Limonium tetragonum can be Limonium tetragonum and/or Limonium tetragonum.
In the preparation method of the limonium japonicum extract, the volume mass ratio of the limonium japonicum to the solvent is preferably 10-40 mL/g.
Wherein the gentian is typically a plant of the genus Gentiana, preferably Gentiana alpina Pall (Gentiana algidapal), Gentiana lantana plantain (Gentiana Veitchiorum Hemsl), Gentiana griffonia (Gentiana jamesii Hemsl.), Gentiana grandiflorum (Gentiana major kanchenyi kantz), Gentiana macrophylla (Gentiana taliensis balf. f. & Forrest), Gentiana multiflora (Gentiana striolata t. n.ho.), Gentiana nobilis (gentianella Franch), Gentiana lutea (Gentiana grandiflora), Gentiana crassipes (Gentiana grandiflora), Gentiana maculata (Gentiana grandiflora), Gentiana lutea (Gentiana grandiflora), Gentiana scabra bungeana, Gentiana japonica (Gentiana grandiflora), Gentiana scabra (Gentiana grandiflora), Gentiana japonica (Gentiana grandiflora), Gentiana scabra bunge (Gentiana grandiflora), Gentiana grandiflora (Gentiana grandiflora) Gentiana himalayana (Gentiana venusta (g. don) wall. ex Griseb.), Gentiana minor (Gentiana parka h. smith), Gentiana sinkiangensis (Gentiana karelinii Griseb.), Gentiana fraxinkiana (Gentiana waluewii Regel & Schmalh.), Gentiana elegans (Gentiana bella Franch. ex hemsl.), Gentiana argentata (Gentiana albicalyx Burk.), Gentiana rubiginea (Gentiana scandala hayata), Gentiana yunnanensis (Gentiana yunnanensis Franch.), Gentiana yunnanensis Franch., Gentiana chinensis sanensis kusanz, Gentiana peak (Gentiana grandiflora) and Gentiana alpina (Gentiana japonica, Gentiana alpina t.s, Gentiana alpina japonica, Gentiana alpina germanica.
In the preparation method of the gentian extract, the solvent is preferably 50-95% by volume of ethanol water solution, and more preferably 70% by volume of ethanol water solution.
In the preparation method of the gentian extract, the volume mass ratio of the gentian to the solvent is preferably 15-30 mL/g.
Wherein, the resin can be macroporous resin which is conventionally used in the field, and D101 resin or AB-8 resin is preferred.
The extraction is preferably ultrasonic extraction, stirring extraction or countercurrent circulation extraction, the time of ultrasonic extraction is preferably 0.5-1.5 hours, the time of stirring extraction is preferably 1.5-2 hours, and the time of countercurrent circulation extraction is preferably 0.3-1 hour. The extraction is most preferably ultrasonic extraction for 1 hour.
Wherein, the water is preferably deionized water.
In a preferred embodiment, the plant extract composition of the present invention is composed of the above-mentioned limonium japonicum extract and gentian extract.
The invention also provides the application of the plant extract composition as a tyrosinase inhibitor or an antioxidant activator; further preferably in the preparation of skin care or cosmetic products.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The positive progress effects of the invention are as follows: the preparation method of the plant extract composition is simple, the prepared plant extract composition can be used as a tyrosinase inhibitor or an antioxidant active agent, and the plant extract composition can be matched with an external agent to be used as an anti-aging agent for preventing skin aging and maintaining a young and healthy skin state.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
Example 1
Preparation of Limonium tetragonum extract
100g of commercially available lukedflower variegate flower and whole herb (Limonium sinense (Girard) Kuntze)) are dried, crushed, mixed and soaked with 2000mL of 70% ethanol solution (v/v, all ethanol solutions in the invention are in volume concentration), extracted for 1 hour by ultrasonic assistance, filtered, and the filtrate is subjected to reduced pressure to remove the solvent, so that 20g of the lukedflower variegate crude extract is obtained.
Taking a crude extract of the limonium, dissolving the crude extract with deionized water, loading the extract to an AB-8 resin packed column, performing gradient elution with deionized water, 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol and 95% ethanol in sequence (the percentage is volume percentage), respectively collecting eluent, removing ethanol and water under reduced pressure, weighing, and respectively obtaining extracts of the limonium of 11g of a water elution part, 2.0g of a 20% ethanol elution part, 2.2g of a 40% ethanol elution part, 2.1g of a 60% ethanol elution part, 1.1g of an 80% ethanol elution part and 1.0g of a 95% ethanol elution part.
Example 2
Determination of total flavone content in Limonium tetragonum extract
The total flavone content of the limonium sinense extract prepared in example 1 is measured by using rutin as a standard substance according to a method of the pharmacopoeia of the people's republic of China (2010 version).
The determination steps are as follows: 1) and (3) standard curve preparation: 1ml, 2ml, 3ml, 4ml, 5ml and 6ml of standard substance solution are precisely measured and respectively placed in a 25ml measuring flask, 30% ethanol is added to 6.0ml, and 1ml of 5% sodium nitrite solution (mass percentage concentration) is added. Mixing, standing for 6 minutes, adding 1ml of 10% aluminum nitrate solution (mass percentage concentration), shaking uniformly, and standing for 6 minutes. Adding 10ml of sodium hydroxide test solution, adding 30% ethanol to scale, shaking, standing for 15 min, measuring absorbance at 510nm wavelength by ultraviolet-visible spectrophotometry with corresponding reagent as blank, and drawing standard curve with absorbance as ordinate and concentration as abscissa.
2) The extract of Limonium sinense (Girard.) Kuntze of each elution part prepared in example 1 was completely dissolved in deionized water to prepare a 1mg/mL aqueous solution. A certain amount of sample is placed in a 25ml volumetric flask, the operation is the same as the step 1 from the step of adding 1ml of 5% sodium nitrite solution according to the standard curve preparation steps, the absorbance is sequentially measured, meanwhile, 3ml of sample solution is sampled, the operation is the same as the above except for no sodium hydroxide test solution, the blank is used, and the total flavone content (in terms of rutin) in the limonium sibiricum extract is calculated, and the following table 1 shows.
TABLE 1 Total flavone content of Limonium tetragonum extract
As can be seen from Table 1, the crude extract obtained after the herba Limonii chinensis is extracted contains more flavonoid compounds, the flavone is the main active component of the herba Limonii chinensis, and after the herba Limonii chinensis is separated by the adsorption resin, the flavone enriched at the 40% ethanol elution part of the herba Limonii chinensis is the most, the flavone content is greatly improved compared with that in the crude extract, and the biological activity is correspondingly enhanced.
Example 3
Preparation method of Gentiana alpina extract
50g of commercially available Gentiana alpina (Gentiana algida Pall.) is dried and crushed, mixed with 1000mL of 70% ethanol solution (v/v, all ethanol solutions in the invention are in volume concentration) for soaking, ultrasonic-assisted extraction is carried out for 1 hour, filtration is carried out, and 7.0g of rough extract of Gentiana alpina is obtained after the filtrate is subjected to reduced pressure solvent removal.
Taking the rough extract of the gentiana alpina, dissolving the rough extract by using deionized water, loading the rough extract to an AB-8 resin packed column, sequentially carrying out gradient elution by using deionized water, 30% ethanol, 70% ethanol and 95% ethanol (the percentage is volume percentage), respectively collecting eluent, removing ethanol and water under reduced pressure, and weighing to respectively obtain the gentiana alpina extract with a water elution part of 4.7g, a 30% ethanol elution part of 0.6g, a 70% ethanol elution part of 1.0g and a 95% ethanol elution part of 0.7 g.
Example 4
Determination of total flavone content in gentiana alpina extract
The total flavone content in the gentiana alpina extract prepared in example 3 was measured according to the method of the pharmacopoeia of the people's republic of china (2010 version) using rutin as a standard.
The determination steps are as follows: 1) and (3) standard curve preparation: 1ml, 2ml, 3ml, 4ml, 5ml and 6ml of standard substance solution are precisely measured and respectively placed in a 25ml measuring flask, 30% ethanol is added to 6.0ml, and 1ml of 5% sodium nitrite solution (mass percentage concentration) is added. Mixing, standing for 6 minutes, adding 1ml of 10% aluminum nitrate solution (mass percentage concentration), shaking uniformly, and standing for 6 minutes. Adding 10ml of sodium hydroxide test solution, adding 30% ethanol to scale, shaking, standing for 15 min, measuring absorbance at 510nm wavelength by ultraviolet-visible spectrophotometry with corresponding reagent as blank, and drawing standard curve with absorbance as ordinate and concentration as abscissa.
2) The gentiana alpina extract from each elution site prepared in example 3 was completely dissolved in deionized water to prepare a 1mg/mL aqueous solution. Placing a certain amount of sample into a 25ml volumetric flask, and performing the same operation as the step 1 from the step of adding 1ml of 5% sodium nitrite solution according to the standard curve preparation steps, sequentially measuring the absorbance, simultaneously sampling 3ml of sample solution, performing the same operation except for adding no sodium hydroxide test solution, and taking the blank to calculate the content (in terms of rutin) of the total flavonoids in the gentiana alpina extract, as shown in the following table 2.
TABLE 2 Total Flavonoids content of Gentiana alpina extract
As shown in Table 2, after separation by the adsorption resin, the 70% ethanol elution part of Gentiana alpina has the most abundant flavones, which are greatly improved in flavone content compared with the crude extract, and the bioactivity of the Gentiana alpina is correspondingly enhanced.
Example 5
The limonium japonicum extract obtained by eluting with 40% ethanol prepared in the method of example 1 and the gentiana alpina extract obtained by eluting with 70% ethanol prepared in example 3 are mixed according to the mass ratio of 10: 1. 2: 1. 1: 1. 1:2 and 1:10, preparing the mixture, and then completely dissolving the mixture in deionized water to prepare 1mg/mL aqueous solution for determining tyrosinase inhibitory activity, wherein arbutin is used as a reference substance.
The determination steps are as follows: mixing phosphate buffer solution (pH6.8, 30mM)70 μ L, 1.66mM tyrosinase solution 80 μ L, and sample solution 80 μ L, incubating in 37 deg.C constant temperature water tank for more than 5 min, adding tyrosinase solution (125U/ml)10 μ L, maintaining at 37 deg.C for 10min, and measuring absorbance A at 475nm wavelength with enzyme labeling instrument475. The absorbance was also measured as a reference solution by replacing the sample aqueous solution with deionized water, and the results are shown in Table 3.
The tyrosinase activity inhibition rate is calculated by the formula: inhibition ratio (%) ═ a0-(A475-B))/A0× 100% wherein A is0Is the absorbance of a reference solution, A475Is the absorbance of the sample solution, and B is the absorbance of the sample blank solution.
TABLE 3 determination of tyrosinase inhibitory Activity
As can be seen from table 3, the composition of the limonium japonicum extract and the gentiana alpina extract has a synergistic inhibition effect on tyrosinase, and when the mass ratio of the limonium japonicum extract to the gentiana alpina extract is 1:1, the tyrosinase inhibition effect is strongest, and the inhibition rate reaches 96.0 percent at most.
Example 6
The extract of the Gentiana alpina prepared by the method of example 1 and the extract of the Gentiana alpina prepared by the method of example 3 and eluted by 40% ethanol are respectively prepared according to the mass ratio of 10:1, 2: 1, 1:2 and 1:10, then the Gentiana alpina extract is completely dissolved by deionized water to prepare 0.01mg/mL aqueous solution, the activity of scavenging free radicals and resisting oxidation is determined by a DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine) method, the determination step is that the Gentiana alpina extract is respectively prepared by deionized water to prepare 0.01mg/mL aqueous solution, 2mL is transferred into a 10mL test tube with a plug, and 2mL of DPPH ethanol solution (2 × 10) is added-4mol/L), mixing well, standing at room temperature for 30min, and measuring 517nm wavelength with spectrophotometerAbsorbance A of517(ii) a Simultaneously measuring the absorbance A of 2mL of extract solution mixed with 2mL of ethanol0Absorbance C after 2mL of water mixed with 2mL of DPPH ethanol solution and absorbance C after 2mL of water mixed with 2mL of ethanol solution0. The measurement is carried out in three times in parallel, the average value is taken, the free radical clearance rate is calculated according to the following formula, and the larger the clearance rate is, the stronger the antioxidant capacity of the extract is. The results are shown in table 4 below:
radical scavenging rate (%) - (1- (a)517-A0)/(C-C0)]×100％
TABLE 4 detection results of radical scavenging activity by DPPH method
As can be seen from table 4, the combination of the extract of limonium and the extract of gentiana alpina has antioxidant activity, but does not show a good synergistic effect.
Claims (10)
1. The plant extract composition is characterized by comprising a limonium japonicum extract and a gentian extract, wherein the mass ratio of the limonium japonicum extract to the gentian extract is (1:1) - (10: 1);
the limonium sinense kuntze extract is prepared by the following preparation method: (1) mixing the limonium sinense kuntze with a solvent, extracting, filtering, and removing the solvent under reduced pressure to obtain a limonium sinense kuntze crude extract, wherein the solvent is an ethanol water solution with the volume percentage of 60-80%; (2) dissolving the crude extract of herba Limonii chinensis in water, loading into a resin packed column, eluting with water and ethanol solution in sequence, collecting eluates, respectively, and removing ethanol and water under reduced pressure to obtain the final product, wherein the resin is D101 resin or AB-8 resin; the limonium japonicum extract is an extract obtained by eluting with 40% ethanol water solution; the percentage is volume percentage;
the gentian extract is prepared by the following preparation method: (1) mixing radix gentianae and a solvent, extracting, filtering, and removing the solvent under reduced pressure to obtain a radix gentianae crude extract, wherein the solvent is an ethanol water solution with the volume percentage of 50-95%; (2) dissolving the rough extract of the gentian in water, loading the rough extract into a resin packed column, eluting with water and ethanol solution in sequence, collecting eluates respectively, and removing ethanol and water under reduced pressure to obtain the gentian extract, wherein the resin is D101 resin or AB-8 resin; the gentian extract is an extract obtained by eluting with 70% ethanol water solution; the percentages are volume percentages.
2. The plant extract composition of claim 1, wherein the mass ratio of the limonium japonicum extract to the gentian extract is 1: 1.
3. The plant extract composition as claimed in claim 1, wherein in the preparation method of the limonium japonicum extract, the ethanol solution is eluted by gradient elution with 10% to 30% ethanol water solution, 31% to 50% ethanol water solution, 51% to 70% ethanol water solution, 71% to 90% ethanol water solution and 91% to 99% ethanol water solution in concentration, and the percentage is volume percentage;
and/or in the preparation method of the gentian extract, the ethanol solution elution is performed by sequentially performing concentration gradient elution with 10-50% ethanol water solution, 51-90% ethanol water solution and 91-99% ethanol water solution, and the percentage is volume percentage.
4. The plant extract composition of claim 3, wherein the extract of Limonium tetragonum is prepared by eluting with a gradient of 20% aqueous ethanol, 40% aqueous ethanol, 60% aqueous ethanol, 80% aqueous ethanol and 95% aqueous ethanol, in volume percent;
and/or, in the preparation method of the gentian extract, the ethanol solution elution is sequentially performed with 30% ethanol water solution, 70% ethanol water solution and 95% ethanol water solution in a concentration gradient manner; the percentages are volume percentages.
5. The plant extract composition of claim 1, wherein the blood-enriching herb is hematinicHerb of Limonium genus (Buxue Cao)Limonium sinense (Girard) Kuntze) Herb of Limonium bicolor (Buxue Bigher)Limonium bicolor (Bag.) Kuntze) Kashi Buxue Cao (Kashi Buxue Cao)Limonium kaschgaricum (Rupr.) Ik.-Gal.)Buxue Cao (herba Limonium Japonici)Limonium gmelinii (Willd.) Kuntze) Herba seu radix Tetrastigmatis HypoglauciLimonium drepanostachyum Ik.- Gal.)Herb of common Flowery Buxue (herb of common Flowery Branch)Limonium flexuosum (L.) Kuntze) Chinese medicinal herb of woody Buxue (Buxue Cao)Limonium suffruticosum (L.) Kuntze) Huichou Liaoxue herb (Buxue Cao)Limonium roborowskii Ik.-Gal.) Blood-tonifying herbs of tobacco pipe (Limonium franchetii (Debx.) Kuntze) Coral Buxue Cao (coral Buxue Cao)Limonium coralloides (Tausch) Lincz.) Limonium wrightii (Chinese lobelia herb)Limonium chrysocomum (Kar. & Kir.) Kuntze) Jinghe Liangxue Cao (blood-nourishing herb for Jinghe)Limonium leptolobum (Regel) Kuntze) Buxue Cao (Buxue Cao) for reproducing branchesLimonium myrianthum (Schrenk)) Hibiscus mutabilis (Hibiscus mutabilis)Limonium wrightii (Hance) Kuntze) Buxue Cao (herba Limoii Teneri)Limonium tenellum (Turcz.) Kuntze) Herb of common Buxue (ear leaf)Limonium otolepis (Schrenk) Ktze.) And Limonium aureum (B.Moench.) (Limonium aureum (L.) Hill) One or more of;
and/or the gentian is gentiana alpinaGentiana algida Pall.) Gentian root (radix gentianae plantaginea)Gentiana Veitchiorum Hemsl) Radix Gentianae of Changbai mountain (a Chinese medicinal preparation)Gentiana jamesii Hemsl.) Gentiana scabra Bunge (Gentiana scabra Bunge.)Gentiana szechenyii Kanitz) Gentiana scabra Bunge (Gentiana scabra Bunge)Gentiana taliensis Balf.f. & Forrest) Gentiana lutea (Gentiana lutea)Gentiana striolata T. N. Ho) Radix Gentianae, radix GentianaeGentiana gentilis Franch) Safflower, gentian (A) and (B)Gentiana rhodantha Franch. ex Hemsl.) Radix Gentianae, andGentiana flavomaculata Hayata) Radix Gentianae of Crassulaceae (herba Rhodiolae)Gentiana crassula H. Smith) And Ju Hua Shen (Gentiana sarcorrhiza Ling & Ma ex T. N. Ho) Radix Gentianae, Linzhi (Chinese gentian)Gentiana nyingchiensis T. N. Ho) Gentiana tassella root (A)Gentiana panthaica Prain & Burk.) And beautiful gentian (Gentiana formosa H. Smith) And large-leaved gentian (large-leaved gentian)Gentiana macrophylla Pall.) Qinghai-Tibet gentian (A)Gentiana futtereri Diels & Gilg) Gentiana scabra Bunge (Gentiana striolata Kuntze.)Gentiana filisepala T. N. Ho) Tianshan Gentiana macrophylla (root of Gentiana macrophylla)Gentiana tianschanica Rupr.) Gentiana lutea (Chinese gentian)Gentiana clarkei Kusnez.) Xijin gentian (a)Gentiana sikkimensis C. B. Clarke) Radix Gentianae of Himalayan (A) and (B)Gentiana venusta (G. Don) Wall. ex Griseb.) Radix Gentianae, andGentiana parvula H. Smith) Gentian in Xinjiang (A)Gentiana karelinii Griseb.) And large-leaved gentian of Xinjiang (Gentiana walujewii Regel & Schmalh.) Xiuli Gentiana (Chinese gentian)Gentiana bella Franch. ex Hemsl.) Radix Gentianae of Silene calyx (A. Merr.) (B. Merr.) (A. Merr.)Gentiana albicalyx Burk.) Radix Gentianae of Yushan mountain (1)Gentiana scabrida Hayata) Gentiana yunnanensis (a) and (b)Gentiana yunnanensis Franch.) Chinese gentian (Chinese gentian)Gentiana chinensis Kusnez.) Zhufeng gentian (gentiana scabra Bunge)Gentiana stellata Turrill) And Gentianae of purple flowerGentiana syringea T. N. Ho) One or more of (a).
6. The plant extract composition of claim 1, wherein the Limonium tetragonum is Limonium tetragonum of genus Limonium (b)Limonium sinense (Girard) Kuntze) The gentian is gentiana alpina: (Gentiana algida Pall.）。
7. The plant extract composition of claim 1, wherein in the preparation method of the limonium japonicum extract, the volume-to-mass ratio of the limonium japonicum to the solvent is 10-40 mL/g;
and/or in the preparation method of the gentian extract, the volume mass ratio of the gentian to the solvent is 15-30 mL/g;
and/or the extraction is ultrasonic extraction, stirring extraction or countercurrent circulation extraction;
and/or the water is deionized water.
8. The plant extract composition of claim 1, wherein the gentian extract is prepared by a method comprising the steps of dissolving the gentian extract in an aqueous ethanol solution at a concentration of 70% by volume;
and/or, when the extraction is ultrasonic extraction, the time of the ultrasonic extraction is 0.5-1.5 hours;
or, when the extraction is stirring extraction, the stirring extraction time is 1.5-2 hours;
or when the extraction is countercurrent circulation extraction, the countercurrent circulation extraction time is 0.3-1 hour.
9. Use of a plant extract composition according to any one of claims 1 to 8 for the preparation of tyrosinase inhibitors or antioxidant actives.
10. Use according to claim 9, wherein the plant extract composition is used in the preparation of a skin care or cosmetic product.
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| CN1518417A (en) * | 2001-06-21 | 2004-08-04 | 协和发酵工业株式会社 | Process for producing plant extract containing plant powder |
| CN101909592A (en) * | 2007-12-25 | 2010-12-08 | 花王株式会社 | External preparation for skin |
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| US7189419B2 (en) * | 2002-12-17 | 2007-03-13 | Avon Products, Inc. | Use of active extracts to lighten skin, lips, hair, and/or nails |
| CN1740184A (en) * | 2004-08-26 | 2006-03-01 | 中国科学院西北高原生物研究所 | A kind of technology of from the Gentiana medicinal plant, extracting the secoiridoid glycosides product |
| CN100344643C (en) * | 2005-07-21 | 2007-10-24 | 中国科学院昆明植物研究所 | Method for preparing gentiamarin |
| CN102920753A (en) * | 2012-11-05 | 2013-02-13 | 江苏省中国科学院植物研究所 | Dichromatism gmelin sealavender herb extractive and application thereof |
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| CN101909592A (en) * | 2007-12-25 | 2010-12-08 | 花王株式会社 | External preparation for skin |
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