CN106137806A - Comprise the former topical composition drawing rattan and the method for skin lightening - Google Patents
Comprise the former topical composition drawing rattan and the method for skin lightening Download PDFInfo
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- CN106137806A CN106137806A CN201510127506.3A CN201510127506A CN106137806A CN 106137806 A CN106137806 A CN 106137806A CN 201510127506 A CN201510127506 A CN 201510127506A CN 106137806 A CN106137806 A CN 106137806A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
A kind of topical composition comprises the vegetable material from galium.Described topical composition can locally use with blast (or whitening) subjects skin.Generally, described topical composition comprises from the former vegetable material drawing rattan kind, such as extract.Described former rattan is drawn to exist with the amount of effective blast subjects skin.In certain embodiments, the active component of described topical composition is drawn rattan to form by former.In a particular embodiment, described topical composition is drawn rattan to form by former.A kind of beauty method for blast subjects skin includes drawing rattan application to the step of subjects skin by former.Described topical composition is typically be enough to the amount of blast skin and/or time application.
Description
Technical field
The present invention relate generally to for blast subjects skin comprise former draw rattan (Galium
Aparine) topical composition, and be more particularly to comprise the former topical composition drawing rattan
And use the method for described composition blast subjects skin.
Background technology
Human skin coloring is determined by the melanic amount of skin surface and position.Melanin is by depositing
It is dermal-epidermal junction, commonly referred to amino acid tyrosine in melanocytic cell
It is oxidizing to L-DOPA (L-DOPA) and synthesize.Described another way,
Melanin is produced by the melanocyte being present in dermal-epidermal junction, and chemical nature is black
Tyrosine in element cell generates L-DOPA through tyrosinase catalysis oxidation, after through a series of
Enzymatic reaction generates melanin.This series of cell process is commonly referred to melanogenesis.Described oxygen
Change process is by the enzymatic of referred to as tyrosinase.The a series of cell processes being carried out by melanocyte
Commonly referred to melanogenesis.
Melanic amount and type that pigmenting of skin is synthesized by melanocyte regulate and control.Environment because of
Element also can affect skin color.In skin, the melanin of healthy amount effectively absorbs ultraviolet
(" UV ") ray.Described another way, in healthy skin, a certain amount of melanin can
Effectively absorb the ultraviolet radiation in external environment.The exposure that UV ray is increased by skin is general
Increase the amount that melanin generates and speed (with time that skin is exposed under UV ray and strong
The increase of degree, the amount that dermal melanin generates and speed also increase accordingly), and can cause
More black skin (colour of skin intensification), or " sepia ".Local or overall pigmentation illness,
Such as hyperpigmentation or hypopigmentation, can be derived from many factors, including internal swash
Element level, diet, genetic disorder and drug therapy.Common pigmentation illness includes yellowish-brown
Spot (melisma), freckle and hickie.
Various compositions have been formulated to solve pigmentation illness, and have for example been expected with
In treatment hyperpigmentation and/or hypopigmentation.This type for the treatment of is commonly known as " skin
Blast ", " skin-whitening " or " skin lightening ".There are several purposes of skin-whitening agents.Example
Such as, blast senile plaque expelling (freckle or Aging color spot), desalination, or prevention Caucasian and
The melanism (that is, maintaining bright/white colour of skin) of Asian skin.Some in these preparations include
Tyrosinase inhibitor such as quinhydrones, vitamin C, kojic acid, ursin, glutathione, half
Cystine, lactic acid, forulic acid, niacinamide, and plant extracts such as black bearberry and mulberry tree carry
Take thing etc..But, some of these compounds, such as quinhydrones and kojic acid, there is side effect,
Including the cytotoxicity of skin irritatin, acute dermatitis and Skin Cell.
In view of above, still existing provides other skin lightening agent, particularly highly effective skin blast
The chance of agent.Most specifically, still there is the effective skin lightening agent of offer and by natural material
Add composition to solve the chance of the problem related to Conventional compounds such as quinhydrones.
Content of the invention
Disclose a kind of topical composition.Described topical composition can locally use with blast (or
Whitening) subjects skin.Described topical composition comprises former to draw rattan.Described former draw rattan with
The amount of effective blast subjects skin exists.In certain embodiments, described topical composition
Active component drawn rattan to form by former.In a particular embodiment, described topical composition by
Former rattan is drawn to form.
Also disclose the beauty method of a kind of blast subjects skin.The method includes applying former drawing
Draw rattan to the step of subjects skin.Described topical composition is typically be enough to the amount of blast skin
And/or time application.
Brief description
Other advantages of the disclosure will readily appreciate that, because by examining with reference to described below and combination
Consider accompanying drawing and become better understood, in accompanying drawing:
Fig. 1 is the line chart illustrating cell toxicity data (" data 1 ")；
Fig. 2 is the line chart illustrating tyrosinase inhibition rate data (" data 2 ")；
Fig. 3 is the line chart illustrating B16 cell suppression data (" data 3 ")；With
Fig. 4 is the line chart illustrating melanin eliminator rate data (" data 4 ").
Detailed description of the invention
Disclose a kind of topical composition (" composition ").Described composition is used for skin lightening
(or whitening).More specifically, described composition can be used for the skin of blast experimenter.Tested
Person is typically people, and can include the men and women at each age.Described composition is not limited to
Particular subject or the skin site of experimenter.For example, described composition can be answered by a people
With to its face, neck, arm, hand, chest, trunk, leg, pin etc. or its any combination.This type of skin
Skin region can be hyperpigmentation or hypopigmentation.Use the side of described composition
Method is described further below.
It is surprisingly found that galium (genus Galium) and can be used for skin lightening.Tool
Body ground, does not carries the baby or is limited to any particular theory, it is believed that the extract of galium reduces
And/or suppression melanin generates.For minimizing/suppression alternatively or additionally, it is believed that galium
Extract eliminate melanin.The composition of the disclosure generally comprises planting from galium
Thing, more generally from the extract of the former plant drawing rattan kind.As used herein, to drawing
Mentioning of rattan can draw rattan to be interchangeably used with former, and vice versa.Based on finding herein,
The embodiment of the disclosure relates generally to topical composition, and it comprises to be enough to reduce/suppress have phase
In requisition for the amount and/or be enough to that generates of experimenter's melanin eliminate in requisition for experimenter
In the extract of clivers of melanic amount, for example former extraction drawing rattan kind plant
Thing.
In each embodiment, described composition comprises to draw rattan, or former draws rattan.?
In further embodiment, the active component of described composition is by drawing rattan to form, or by former
Rattan is drawn to form.In these embodiments, described composition can be further by one or many
Plant annexing ingredient and/or inactive ingredients composition.In certain embodiments, described composition
By drawing rattan to form, or rattan is drawn to form by former.In all these embodiments, described
Composition can include the vegetable material from galium, including from one or more kinds of examples
Such as the former vegetable material drawing rattan (also known as flower draws rattan (Galium aparine L.)).
Draw rattan to be the annual big genus with herbaceos perennial of Rubiaceae, be present in north
Hemisphere and the temperate zone in the Southern Hemisphere.Have more than 600 kinds and draw rattan, including former draw rattan or " pig calamity
Calamity (Cleaver) ", it is also known as: Cliver, Goosegrass, Catchweed, Stickyweed,
Robin-run-the-hedge, Sticky Willy, Sticky Willow, Velcro Weed and Grip
Grass.Clearvers is annual plant, has sarmentum of crawling, its along landing ground and other
The top set of plant simultaneously grows.They are connected by the little glochild oneself growing from stem and leaf.Stem can
It reaching three feet or longer, and is dihedral or square.Leaf is simple, narrow drapes over one's shoulders pin
Shape is extremely linear and verticillate with 6 to 8.Clearvers has stellular white to light green color
Flower, it occurred in early spring to spring.Described flower is with 2 or 3 packet gatherings, and grows
Outside axil.Seed ball is bur, and it grows gathering 1 to 3 seed together；It
Covered by glochild, glochild sticks to animal skin, helps seed dispersal.
Former draw rattan innately in Europe, North America and Asia be from Britain and Canary Islands to Japan
Wide geographic area.At present, its transplanted throughout the U.S., Canada, Mexico, Central America,
The major part in South America, Australia, some ocean islands and dispersion place, Africa.Former draw rattan
Chemical composition includes: iridoid glycoside such as woodruff thuja acid and 10-10-deacetyl asperulosidic thuja acid,
Asperuloside, monotropein and aucubin, alkaloid such as caffeine, phenolic compound
Such as phenolic acid, anthraquinone derivative such as aldehyde Nordamnacanthal (1,3-quinizarin-2-aldehyde),
Flavonoids and cumarin, organic acids such as citric acid, and orchil.
The scientific classification of this component of described composition is typically following-mesh: and Gentianales
(Gentianales)；Section: Rubiaceae (Rubiaceae)；And genus: draw rattan.Draw rattan
Hybrid between two or more kinds is also possible.In each embodiment, draw the rattan can
Draw the one or more of rattan kind to be selected from.In many embodiments, draw rattan to include or
It is former to draw rattan (or former draw rattan (G.aparine)).
Any part drawing rattan plant can be used for producing the material using in composition, including but
It is not limited to the extract of root, stem, rhizome, leaf, flower, fruit and/or these parts.La La
Rattan can in original form, suspended form, dehydrated form, conc forms or form of extract use.
Generally, rattan is drawn to be to be dried or liquid form.This component of composition and/or other components
Extract can be obtained by general extraction methods known in the art, for example by water (for example
Steam) extract or extracted by solvent (such as alcohol).The composition of the disclosure is not limited to specific
Extracting method.Exemplary extracting method is described below.
In many embodiments, composition comprises from the former extract drawing rattan kind.Such as this
Literary composition uses, and mentions, to " drawing boisiana extract ", the extraction referring generally to containing from galium
Thing, described include from individually (i.e. " former draw boisiana extract ") or with one or more other
The former of galium combination planted draws rattan kind.Draw boisiana extract can business available from various come
Source.Additionally, the suitable boisiana extract that draws can be obtained by using any conventional extraction techniques.
Many is had to can be used for producing the extracting method of the extract being suitable for described composition.These sides
Method includes but is not limited to the extraction side disclosed in the U.S. Patent number 7,897,184 of Rana et al.
Method, it is incorporated herein by reference in their entirety at this, and below with reference to some extracting methods
Partly replicate.Although the Extraction solvent describing refers specifically to ethanol, it should be appreciated that, other
Alcohol, such as but not limited to isopropanol, ethyl alcohol and/or methylol can supplement or substitute ethanol
And use.Exemplary alcohols solvent includes but is not limited to C1To C4Alcohol, such as methyl alcohol, ethanol,
Propyl alcohol, isopropanol and butanol；Water-alcohol or the mixture of alcohol and water, including water-ethanol
(hydro-ethanol)；Polyhydroxy-alcohol, such as propane diols and butanediol；And fatty alcohol.Permissible
Use any one of these alcoholic solvents.Other solvents, such as but not limited to acetone also can be made
Use for Extraction solvent.Solvent-water the admixture of any ratio, such as alcohol-water can also be used
And/or acetone-water admixture.
In an example, it is possible to use Solvent Extract methods technology obtains and draws boisiana extract.
In another example, it is possible to use solvent series classification separates to obtain draws boisiana extract.Also
Total water-ethanol extractive technique can be used to obtain and to draw boisiana extract.It is said that in general, its
It is referred to as disposable extraction.The extract producing in this process can contain the extraction material of wide scope
Present in phytochemical, including fat-soluble and water-soluble plant chemical composition.Carry in collection
After taking solution, evaporate solvent, obtain extract.
Total alcohol extract can also be used.This technology uses ethanol as solvent.This extraction skill
The extract that art produces can also include fat-soluble and/or lipophilic in addition to water soluble compound
Compound.Also total methyl alcohol can be used in a similar manner to extract, there is similar result.At each
In embodiment, obtained draw rattan to extract by the former vegetable material drawing rattan kind of alcohol extracting
Thing.
Another example that can be used for obtaining the extractive technique drawing boisiana extract is supercritical fluid
CO 2 supercritical extracts (" SFE ").In this extraction procedure, material to be extracted is not sudden and violent
It is exposed to any organic solvent.But, Extraction solvent is supercriticality (such as ＞ 31.3 DEG C
With ＞ 73.8 bar) under carbon dioxide (CO2), contain and be with or without conditioning agent.Art technology
Personnel are it will be appreciated that temperature and pressure condition can change to obtain optimal extract productivity.Should
Technology produces fat-soluble and/or lipophilic compound extract, is similar to total hexane and acetic acid second
Ester extractive technique, it also can use.
Drawing boisiana extract any amount can add composition, condition is that it is tested with effective blast
The amount of person's skin exists.Generally, boisiana extract is drawn to reduce (or suppression) experimenter with effective
Dermal melanin generates and/or eliminate melanic amount to be existed.In each embodiment, draw
Boisiana extract is drawn to exist with the amount of about 10 to about 300 μ g/mL compositions.In specific embodiment party
In case, draw boisiana extract with about the 10th, about the 20th, about the 25th, about the 50th, about 75 or about 100 μ g/mL
The amount of composition exists.Further contemplate each subrange of about 10 to about 300 μ g/mL compositions
And amount, and the amount less than or greater than this tittle.
The amount drawing boisiana extract present in composition can be depending on several factor, including wish
Melanin suppression level, the melanin suppression level in specific extraction thing or composition and
Other factors.In certain embodiments, based on 100 weight portions (" pbw ") composition,
Draw boisiana extract with about 0.01 to about 20pbw amount exist.In further embodiment,
Based on 100pbw composition, draw boisiana extract with about 0.05 to about 10pbw amount exist.
Further contemplate each subrange and the amount of about 0.01 to about 20pbw, and be less than or greater than these
The amount of amount.It is optionally used for other extracts of composition or composition is described in Dornoff's et al.
U.S. Patent number 5,747,006, and Leverett et al. U.S. Patent number 5,980,904,
6,994,874,7,060,304,7,247,321 and 7,364,759, the disclosure of which is by quoting
It is integrally incorporated herein.
In certain embodiments, drawing rattan except former, composition also comprises to supplement active component.
Supplementary active component is generally selected from following group: north trifoliate orange (Hovenia dulcis), the root bark of Chinese wolf-berry or
Matrimony vine (Kycii Cortex or Lycium chinense), Thalictrumpetaloideum (Thalictrum
Petaloideum) and combinations thereof.In these embodiments, supplementary active component can come
Extract from north trifoliate orange kind, matrimony vine kind and Thalictrumpetaloideum kind.This type of extract can be with business
Obtain, or obtained by one of said extracted technology.Although optional, but it is believed that this
Supplement any one of active components a bit or combine and can be applied in combination to enter with drawing boisiana extract
One step helps skin lightening.If used, supplementary active component can be as drawing rattan to extract
Described amount uses, such as with about 10 to about 300 μ g/mL compositions or about 0.01 to about
The amount of 20pbw composition.
In certain embodiments, composition does not contains other active materials.Here " other work
Property material " typicallys represent composition and is different from the other kinds of traditional Chinese medicine drawing rattan
(“TCM”；Or " Chinese medicine ").Other kinds of TCM is understood in the art.Other classes
The example of the TCM of type is typically " biological described in international publication number WO 01/22934 A2
Active material ", its content is incorporated herein by reference in their entirety.In certain embodiments, group
Compound can comprise following inactive material.If used, inactive material is different from other classes
The TCM of type.
Can be with compositions formulated to comprise cosmetically acceptable carrier (or medium) preparing
And/or pack and be denoted as increasing skin lightening or whitening, suppression, reduction or minimizing melanin
Generate or pigmentation.Composition can be with local application.The cosmetically example of acceptable carrier
Including but not limited to water, glycerine, wax, various alcohol such as ethanol, propyl alcohol, vegetable oil, mineral
Oil, silicone such as silicone oil, fatty ester, fatty alcohol, glycol, polyglycols or its any combination.
This type of component is generally considered to be inactive ingredients.Final composition can be to be suitable for topical application
In any form of skin, such as but not limited to aerosol spray, gel, creams, dispersion
Liquid, emulsion, foam, liquid, washing lotion, mousse, paster, pomade, pulvis, pump type spray
Mist agent, solid, solution, sticky stick or little towel.Emulsion can include oil-in-water emulsion, Water-In-Oil
Emulsion and silicone bag aqueous emulsion.
Various method known in the art can be used to prepare the composition of the disclosure.In preparation group
In one example of compound, preparation method includes extracting the former vegetable material drawing rattan kind to obtain
The former step drawing boisiana extract.Preparation method also includes combining former draws boisiana extract and beauty treatment
The step of one or more of for example above-mentioned carrier of upper acceptable carrier.Can use conventional raw
Produce method and apparatus such as blender, plunger etc. and carry out combination partner.
Composition can be used for skin lightening in every way.As an example, it is used for blast experimenter
The beauty method of skin includes the former step drawing rattan to be applied to subjects skin.Former draw rattan
Can apply in every way, including composition is applied to subjects skin.Can be by composition
It is directly or indirectly applied to skin, for example, pass through hand, spreader, paster etc..
Can on demand, every day, one day several times or any suitable scheme administration composition, with
Just results needed is realized.In beauty method, topical application frequency can depend on several factor,
Including required melanin generates suppression level.It is said that in general, scheme includes once a day or two
Secondary application composition is applied and/or application in evening to include morning in skin.Should in applying every time
Amount for the composition of skin can depend on several factor, including wish result level and
Concrete composition.
Illustrate following example of disclosure composition and method expected illustrate rather than
Limit the present invention.
Embodiment
Described below is exemplary extracting method.This extracting method is used for obtaining disclosure combination
The former of thing draws boisiana extract.This extracting method is properly termed as methyl alcohol classification separation or methyl alcohol carries
Take.It will be appreciated by those skilled in the art that other extracting methods can be used for obtaining carrying of wanting
Take thing, including those described above.To determine related other extracting methods of skin lightening effect and divide
Analysis method (such as assay method) is described in the international publication number WO of Rana et al.
2011/019468A2, WO 2011/109139A2 and WO 2013/169634A2, it is public
Open content to be incorporated herein by.
Exemplary extracting method
Any material or the extract that use should be powder types.Weigh according to following table and record
(to immediate 0.1g) sample entrance flask:
| Charging | Extract | |
| Plant/botanical weight (g) | 50 | 10 |
| Flask volume (mL) | 500 | 150 |
| Methyl alcohol volume (mL) | 300 | 60 |
Add stirring rod and pour the methyl alcohol measured shown in table into flask.Use aluminum sealing lid flask
Opening.Flask is placed on magnetic agitation plate and use slowly/moderate stirring rate stir at least
12 hours.Sample is kept to avoid direct light.Take out flask at room temperature ultrasonic from agitating plate
Process one hour, vortex once in a while.
It by GF/A Filter paper filtering sample solution, is directly entered 500mL round bottom kieldahl flask.
Use remaining solvent in Rotary Evaporators evaporation round bottom kieldahl flask.Reduce solvent volume extremely little
In 10mL.Glass pipet is used to be transferred to concentrated extract (being still that liquid form)
The scintillation vial (uncovered is weighed) weighed in advance.Methyl alcohol is used to enter one as needed for transfer purpose
Step dilution.
Pipe is placed under nitrogen evaporimeter and uses slow nitrogen stream reduce volume may to minimum.Recommend
Bath temperature be 40 DEG C.From nitrogen evaporimeter take out pipe and its uncovered is put into drier until
It is dried (about 12 hours).In record scintillation vial, the final dry weight (uncovered) of fraction (is passed through
Difference calculates).Cap and be stored in refrigerator to pipe for further.
Use aforementioned extracting method obtain former draw boisiana extract after, carry out various testing and producing
Raw corresponding data set is to determine the former effect drawing boisiana extract in terms of skin lightening.In phase
Close part, use efficacy determinations well known by persons skilled in the art and method evaluate cytotoxicity,
Tyrosinase suppression, B16 cell suppression and melanin eliminator.Tabular data be provided in
Under data the 1st, data the 2nd, data 3 and data 4 collect and respective Fig. 1, the 2nd, 3
With 4.
Data 1
Former draw cytotoxicity-CTG (quinhydrones is as reference) in B16F10 for the rattan
Promega Corporation's(CTG) luminescent cell vigor is surveyed
Surely it is the homogeneous method measuring viable cell number in culture, based on as metabolic active cells
The ATP's indicating and existing is quantitative.According to the CTG by illustrating in data 1 and Fig. 1
The safety evaluatio based on cell, former draw rattan there is no cytotoxicity risk.
According to vitro bioassay data, find that the former medicinal herbal extract drawing rattan has skin and increases
Bright and that depigmentaton is calm effect.Apply the mensuration based on cell by model cell B16F10,
Monitoring tyrosinase activity suppression, B16 cell suppression and the melanin eliminator activity existing.
The mensuration based on model cell B16F10 cell of the type is known to those skilled in the art
's.
Ursin is considered tyrosinase activity suppression and B16 cell suppresses both positives
Comparison, and quinhydrones is used as the positive control of the melanin eliminator activity existing.Sum up following description
Result, former draw rattan to have in testing in vitro as the calm reagent of skin lightening or depigmentaton
Prominent activity is without cytotoxicity risk.
Data 2
Former draw tyrosinase inhibition rate (ursin is as reference) in B16F10 for the rattan
Former draw rattan
Ursin
Former draw boisiana extract to play the suppression of excellent tyrosinase in B16F10 cell to live
Property, relative to the positive control ursin of same concentrations, have under 100 μ g/mL concentration
The inhibiting rate of 97.3%.Specifically, as illustrated in data 2 and Fig. 2, former draw
Rattan quite or gets well with ursin effect in terms of tyrosinase inhibitory activity in B16F10 cell
Many.
Data 3
Former draw rattan in B16F10 B16 cell suppression (ursin is as reference)
Former draw rattan
Ursin
In B16F10 cell in B16 cell inhibitory activity, relative to positive control black bearberry
Glycosides, the former inhibiting rate drawing rattan is 96.1% under 100 μ g/mL.Specifically, such as data 3
Illustrate with Fig. 3, former draw rattan B16 cell inhibitory activity in B16F10 cell
Aspect and ursin effect are quite or much better.This means former to draw rattan to have the ability strong interruption
Melanin synthesis process in (blocking-up) or minimizing B16F10 cell.Under this data set typically passes through
The assay method that will list of literary composition and obtain.
B16 cell suppression measures:
Purpose: the standard that in this program description B16F10 cell, B16 cell suppression measures
Method.
Clone: B16-F10 (ATCC#)
Material:
RPMI 1640 (GIBCO#22400-089 lot number 1006397) for B16
Hyclone (GIBCO#10099-141)
Trypsase-EDTA (GIBCO#25200-072)
·1M NaOH
24 orifice plates (Corning)
Program:
1st day: bed board cell
1. trypsinized dish measure cell density.
2. the density diluting cells slurry with 18,000 cells/ml is extremely volume required.
3. by 1ml/ porocyte slurry distribution to assay plate.
4. with 37 DEG C, 5%CO under humidified condition2Hatch assay plate 24 hours.
2nd day: add test compound
1. prepare reference and test compound solution (200 ×) according to plate figure.
2. 5 μ l compounds are transferred to assay plate (ultimate density: 1 ×).
3. with 37 DEG C, 5%CO under humidified condition2Hatch plate 72 hours.
5th day: make plate imaging
1. remove culture medium.
2. add 150 μ l 1M NaOH to assay plate.
3. at 80 DEG C, hatch plate 30min.
4. 140 μ l solution are transferred to UV plate.Test 400nm signal.
5. 5 μ l solution are transferred to UV plate.Add 200 μ l BCA reagent to each hole simultaneously
Hatch 20 min at 37 DEG C.Test 562 nm signals.
Data process:
Use GraphPad.
% suppression=(peak signal-compound signal)/(peak signal-minimum signal) × 100.
Peak signal is available from the effect of DMSO.
Minimum signal is available from 200 μ g/ml ursin.
Data 4
Former draw melanin eliminator rate (quinhydrones is as reference) in B16F10 for the rattan
Former draw rattan
Quinhydrones
In the melanin eliminator existing measures, relative to positive control quinhydrones at 100 μ g/mL
When 73.5% elimination factor, discovery B16F10 cultured cells in 33.9% pigment add 100
μ g/mL is former draws rattan to eliminate after 72 hours.This means former to draw rattan to be also used for melanin
Eliminate.This data set is typically obtained by the following assay method that will list.
The melanin eliminator existing measures:
Purpose: the standard method that in this program description B16 cell, melanin eliminator measures.
Clone: B16-F10 (ATCC#)
Material:
RPMI 1640 (GIBCO#22400-089 lot number 1006397) for B16
Hyclone (GIBCO#10099-141)
Trypsase-EDTA (GIBCO#25200-072)
·1M NaOH
24 orifice plates (Corning)
Program:
1st day: bed board cell
1. trypsinized dish measure cell density.
2. the density diluting cells slurry with 18,000 cells/ml is extremely volume required.
3. by 1ml/ porocyte slurry distribution to assay plate.
4. with 37 DEG C, 5%CO under humidified condition2Hatch assay plate 24 hours.
2nd day: cell process
1. 10 μM of forskolins and 50 μM of 8-MOP are added in every hole.
2. with 37 DEG C, 5%CO under humidified condition2Hatch assay plate 72 hours.
5th day: add test compound
1. change fresh culture, every hole 1ml.
2. prepare reference and test compound solution (200 ×) according to plate figure.
3. 5 μ l compounds are transferred to assay plate (ultimate density: 1 ×).
4. with 37 DEG C, 5%CO under humidified condition2Hatch plate 72 hours.
8th day: make plate imaging
1. remove culture medium.
2. add 150 μ l 1M NaOH to assay plate.
3. at 80 DEG C, hatch plate 30min.
4. 140 μ l solution are transferred to UV plate.Test 400nm signal.
5. 5 μ l solution are transferred to UV plate.Add 200 μ l BCA reagent to each hole simultaneously
Hatch 20min at 37 DEG C.Test 562nm signal.
Data process:
Use GraphPad.
% suppression=(peak signal-compound signal)/(peak signal-minimum signal) × 100.
Peak signal is not available from having compound effects cell.
Minimum signal is available from 10 μM of forskolins and 50 μM of 8-MOP.
Be appreciated that claims be not limited to describe in detail described in clear and definite and specific chemical combination
Thing, composition or method, it can be in the particular implementation side falling within the scope of the appended claims
Change between case.For being used for herein describing each embodiment special characteristic or aspect institute foundation
Any marlcush group for, it is understood that different, special and/or unexpected results
Can be available from each member of each marlcush group independent of every other Ma Kushi member.Horse
Each member in assorted group of storehouse can by alone or in combination according to and provide to claims
In the range of specific embodiments enough support.
It is also to be understood that describe any scope of institute's foundation in each embodiment of the present invention and sub-model
Enclose independent and totally fall into scope, and being understood to describe and cover to include
Wherein all and/or partial value is in interior all scopes, even if this type of value is not herein clearly
Write.Those skilled in the art easily find, the scope enumerated and subrange fully describe and real
Show each embodiment of the present invention, and this type of scope and subrange can be further depicted as
Become 1/2nd, 1/3rd, 1/4th, five related/first-class.It is only used as one
Example, the scope of " 0.1 to 0.9 " can be further depicted as into less 1/3rd, and i.e. 0.1
To 0.3, middle 1/3rd, i.e. 0.4 to 0.6, and bigger 1/3rd, i.e. 0.7 to
0.9, it is individual and generally within the scope of the appended claims, and can individual and/or
Generally by according to and provide to enough the propping up of specific embodiments in scope
Hold.Additionally, with regard to the language limiting or modifying scope, such as " at least ", " being more than ", " little
In ", " no more than " etc., it is understood that this type of language pack enclosed tool scope and/or the upper limit or lower limit.Make
For another example, the scope of " at least 10 " is intrinsic include at least 10 to 35 subrange, at least
The subrange of 10 to 25, subrange of at least 25 to 35 etc., and each subrange can
With individual and/or generally by according to and be embodied as in providing to scope
Enough supporting of scheme.Finally, in the range of disclosed individual numerical value can by according to and provide right
In scope, specific embodiments enough supports.For example, the model of " 1 to 9 "
Enclose and include each individual integer, such as 3, and include the number of individuals of decimal point (or fraction)
Value, such as 4.1, its can by according to and provide and be embodied as in scope
Enough supporting of scheme.
Describe the present invention herein by way of illustration, and it is understood that made
Term it is contemplated that in words of description character rather than limit.In view of teachings above, the present invention
Many adjust and change is possible.The present invention can be within the scope of the appended claims with not
The mode being same as specifically describing is implemented.Take explicitly into account independent claims and appurtenance herein
Require the theme of all combinations of (individual event and multinomial subordinate).
Claims (20)
1. the topical composition for blast subjects skin, described topical composition comprises
The former of amount of effective blast subjects skin draws rattan.
2. the topical composition for skin lightening, the activity one-tenth of described topical composition
Dividing is drawn rattan to form by former.
3. topical composition as claimed in claim 1 or 2, wherein said topical composition bag
Containing from the former extract drawing rattan kind.
4. topical composition as claimed in claim 3, wherein said former draw boisiana extract with
Effectively reduce melanin in experimenter and generate and/or eliminate the existence of melanic amount.
5. topical composition as claimed in claim 3, wherein said former draw boisiana extract with
The amount of topical composition described in about 10 to about 300 μ g/mL exists.
6. topical composition as claimed in claim 3, wherein said former draws boisiana extract to lead to
Cross the former vegetable material drawing rattan kind described in alcohol extracting and obtain.
7. topical composition as claimed in claim 1 or 2, also comprises cosmetically acceptable
Carrier.
8. topical composition as claimed in claim 7, wherein said cosmetically acceptable
Carrier is selected from the group consisting of: water, glycerine, wax, alcohol, vegetable oil, mineral oil, silicon
Ketone, fatty ester, fatty alcohol, glycol, polyglycols and combinations thereof.
9. topical composition as claimed in claim 1, also comprises selected from the group consisting of
Supplementary active component: trifoliate orange, the root bark of Chinese wolf-berry, lobe core grass of meadow rue and combinations thereof.
10. topical composition as claimed in claim 9, wherein said supplementary active component bag
Containing the extract from trifoliate orange kind, the root bark of Chinese wolf-berry and lobe core grass of meadow rue kind.
11. topical compositions as claimed in claim 10, wherein said supplementary active component
Extract exists with the amount of topical composition described in about 10 to about 300 μ g/mL.
12. topical compositions as described in any one of claim 9 to 11, also comprise beauty treatment
Upper acceptable carrier.
13. topical composition as claimed in claim 12, wherein said cosmetically can accept
Carrier selected from the group that consists of: water, glycerine, wax, alcohol, vegetable oil, mineral oil,
Silicone, fatty ester, fatty alcohol, glycol, polyglycols and combinations thereof.
14. 1 kinds of topical compositions for skin lightening, described topical composition is drawn by former
Rattan forms.
15. claims the 1st, the topical composition described in 2 or 14 any one is tested for blast
The purposes of person's skin.
16. 1 kinds of sides preparing topical composition the 1st, described in 2 or 14 any one for the claim
Method, described method includes extracting the described former vegetable material drawing rattan kind and former draws rattan to carry to obtain
Take the step of thing.
17. methods as claimed in claim 16, also include combining and described former draw rattan to extract
The step of thing and cosmetically acceptable carrier.
18. 1 kinds of beauty methods for blast subjects skin, described method includes applying former
Draw rattan to the step of subjects skin.
19. methods as claimed in claim 18, wherein said applying step limits further
For application topical composition to subjects skin, wherein said topical composition comprises from described
The former extract drawing rattan kind.
20. methods as claimed in claim 19, wherein said former draw boisiana extract to have
Effect reduces melanin in subjects skin and generates and/or eliminate the existence of melanic amount.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510127506.3A CN106137806A (en) | 2015-03-23 | 2015-03-23 | Comprise the former topical composition drawing rattan and the method for skin lightening |
| TW105107746A TW201634045A (en) | 2015-03-23 | 2016-03-14 | Topical composition containing galium aparine and method for brightening skin |
| PCT/CN2016/076205 WO2016150310A1 (en) | 2015-03-23 | 2016-03-14 | Topical composition containing galium aparine and method for brightening skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510127506.3A CN106137806A (en) | 2015-03-23 | 2015-03-23 | Comprise the former topical composition drawing rattan and the method for skin lightening |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106137806A true CN106137806A (en) | 2016-11-23 |
Family
ID=56979048
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510127506.3A Pending CN106137806A (en) | 2015-03-23 | 2015-03-23 | Comprise the former topical composition drawing rattan and the method for skin lightening |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN106137806A (en) |
| TW (1) | TW201634045A (en) |
| WO (1) | WO2016150310A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113200911B (en) * | 2021-05-18 | 2023-05-05 | 云南民族大学 | Quinoline alkaloid compound and preparation method and application thereof |
| CN113214151B (en) * | 2021-05-18 | 2023-05-05 | 云南民族大学 | Anti-rotavirus active compound in Thalictrum cyrtonema and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002179516A (en) * | 2000-12-13 | 2002-06-26 | Pola Chem Ind Inc | Skin-whitening composition |
| CN101022819A (en) * | 2003-07-03 | 2007-08-22 | 薰衣草作物项目有限公司 | Herbal composition |
| CN103784368A (en) * | 2014-01-15 | 2014-05-14 | 广东雅丽洁精细化工有限公司 | Skin care composition and cream with relieving and whitening functions and preparation method thereof |
| CN103906524A (en) * | 2011-04-06 | 2014-07-02 | 玫琳凯有限公司 | Topical skin care formulations comprising plant extracts |
| CN104394839A (en) * | 2012-06-11 | 2015-03-04 | 株式会社爱茉莉太平洋 | Anti-aging cosmetic composition |
-
2015
- 2015-03-23 CN CN201510127506.3A patent/CN106137806A/en active Pending
-
2016
- 2016-03-14 WO PCT/CN2016/076205 patent/WO2016150310A1/en active Application Filing
- 2016-03-14 TW TW105107746A patent/TW201634045A/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002179516A (en) * | 2000-12-13 | 2002-06-26 | Pola Chem Ind Inc | Skin-whitening composition |
| CN101022819A (en) * | 2003-07-03 | 2007-08-22 | 薰衣草作物项目有限公司 | Herbal composition |
| CN103906524A (en) * | 2011-04-06 | 2014-07-02 | 玫琳凯有限公司 | Topical skin care formulations comprising plant extracts |
| CN104394839A (en) * | 2012-06-11 | 2015-03-04 | 株式会社爱茉莉太平洋 | Anti-aging cosmetic composition |
| CN103784368A (en) * | 2014-01-15 | 2014-05-14 | 广东雅丽洁精细化工有限公司 | Skin care composition and cream with relieving and whitening functions and preparation method thereof |
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| 张贵财 主编: "《陇南本草纲要》", 31 January 2012 * |
| 敖恩宝利格 等.: "《瓣蕊唐松草根茎中抗氧化物质的提取研究》", 《时珍国医国药》 * |
| 赵永杰，张静: "《抗氧化剂在个人护理品中的研究进展》", 《日用化学品科学》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| TW201634045A (en) | 2016-10-01 |
| WO2016150310A1 (en) | 2016-09-29 |
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