CN106124358A - A kind of measure the method washing fiber content in sweet sorghum stalk residue - Google Patents
A kind of measure the method washing fiber content in sweet sorghum stalk residue Download PDFInfo
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- CN106124358A CN106124358A CN201610532222.7A CN201610532222A CN106124358A CN 106124358 A CN106124358 A CN 106124358A CN 201610532222 A CN201610532222 A CN 201610532222A CN 106124358 A CN106124358 A CN 106124358A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
- G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The present invention provides a kind of method measuring and washing fiber content in sweet sorghum stalk residue, and step is: the pretreatment of sweet sorghum stalk；Configuration neutral detergent, acid detergent, sulphuric acid；Sample neutral detergent after pretreatment is disappeared and after boiling, is calculated the content of neutral detergent fiber.Again weigh sample, disappear with acid detergent and be calculated acid detergent fiber content after boiling；With being calculated acidic cleaning content of lignin, content of cellulose, hemicellulose level, content of lignin after sulfuric acid treatment.Step of the present invention is simple, can conveniently measure the many kinds of parameters such as neutral detergent fiber content in sweet sorghum stalk residue, and the utilization for its resource provides data and theories integration.
Description
Technical field
The present invention relates to the measuring method of content of material in sweet sorghum stalk, measure sugar grass straw in particular it relates to a kind of
The method washing fiber content in stalk residue, belongs to agricultural technology field.
Background technology
In agricultural production, substantial amounts of biomass castoff, for a long time the resource energy of these garbages can be produced
Change the focus utilizing always research.Sugar grass is a kind of reproducible green energy resource crop, and most research concentrates on profit
Ferment producing and ethanol with the stalk juice of sugar grass, but its straw residue is also a kind of resource, can develop further.
Literature search through prior art finds, Chinese Patent Application No. is CN 201110091984.5, Publication No.
CN 102735566A, entitled: the assay method of acid detergent fiber content in herbage, the readme of this patent is: (1) will weight
Described herbage and the mixing of acidic cleaning liquid that amount is A are placed in preservation bottle, are placed in microwave oven by described preservation bottle and take off
Gas；(2) described preservation bottle carries out sealing be placed in pressure cooker and pressurize, obtain mixed liquor；(3) it is the mistake of B by weight
Filter carries out the mixed liquor that filtration step (2) obtains, and the filter cake obtained is described acid detergent fiber；(4) by described filtration
Device and filtration cakes torrefaction are to constant weight C, then according to formula (I) carries out calculating i.e. obtains acid detergent fiber content described in the most described herbage
ADF (%).This technology is disadvantageous in that: needing to use pressure vessel (pressure cooker) to test, experimental procedure compares
Loaded down with trivial details.Once measure the content that can only calculate acid detergent fiber.
Chinese Patent Application No. is CN201510121434.1, Publication No. CN104655784A, entitled: a kind of mensuration
The method of straw lignocellulose constituent content, the readme of this patent is: the plant straw first choosing material to be tested enters
Row pretreatment, then carries out 72% concentrated sulphuric acid and 4% dilute sulfuric acid two step acidolysis, the lignocellulose component in straw is dropped
Solve, separate, then accurately measured the content of cellulose and hemicellulose by HPLC method, utilize UV method to measure degradation solution
The content of the solvable lignin of middle acid, measures the content of sour insoluble lignin by calcination method.This technology is disadvantageous in that:
Need to use the precision instruments such as high phase chromatograph of liquid (HPLC) and ultraviolet spectrophotometer (UV) to test, experimental procedure ratio
Cumbersome.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide a kind of mensuration and sweet sorghum stalk residue washs fibre
The method of dimension hplc, step is simple, can conveniently measure the neutral detergent fiber content in sweet sorghum stalk residue, acid
Property washing fiber content, acidic cleaning content of lignin, content of cellulose, hemicellulose level, many seed ginsengs such as content of lignin
Number, the utilization for its resource provides data and theories integration.
For achieving the above object, the present invention is by the following technical solutions:
A kind of measure the method washing fiber content in sweet sorghum stalk residue, comprise the steps:
The pretreatment of S1: sweet sorghum stalk: remove root, fringe and foliage portion after being gathered in the crops by sweet sorghum stalk, be dried
Processing, the residue sample of sweet sorghum stalk, for follow-up mensuration；
S2: weigh the residue sample of appropriate described sweet sorghum stalk, disappear with neutral detergent and boil, sucking filtration also rushes with acetone
Wash, obtain the content of neutral detergent fiber after drying；Described neutral detergent is the sodium dodecyl sulfate solution of 25-35g/L；
S3: again weigh the residue sample of described sweet sorghum stalk, disappear with acid detergent and boil, sucking filtration also rushes with acetone
Wash, obtain solid after drying, be calculated acid detergent fiber content；Described acid detergent is hexadecane trimethyl ammonium bromide
Mixed aqueous solution with sulphuric acid；
S4: in the solid that S3 obtains, adding mass percent concentration is the sulphuric acid of 68-75%, and stirring is in the pasty state, quiet
Put, be dried after distilled water flushing and weigh；
S5: the solid arrived of S4, carries out ashing process, weighs, be calculated acidic cleaning lignin after ashing cooling
Content, content of cellulose, hemicellulose level, content of lignin.
Preferably, described S1, step is:
S101: removing root, tassel and leaf after being gathered in the crops by sweet sorghum stalk, by chaser of squeezing the juice, extruding obtains sweet
The juice of Sorghum vulgare Pers. and sweet sorghum stalk residue；
S102: the sweet sorghum stalk residue obtained by S101 air-dries after processing, and sieves and obtain the biology of 40-60 mesh after grinding
Quality sample；
S103: the biomass samples obtained by S102, according to distilled water and biomass samples mass ratio 5-50, biological
Quality sample washs three times, removes sucrose, glucose and the fructose material of residual in biomass samples；
S104: the process carrying out the material after S103 processes, is dried 24 hours, at exsiccator in the baking oven of 60 DEG C
In be cooled to room temperature after, seal up for safekeeping with sealing bag, in order to avoid the degraded that the materials such as cellulase are to biomass samples, result caused by mistake
Difference.
Preferably, described neutral detergent, collocation method is:
Weigh disodiumedetate Na2EDTA, sodium tetraborate Na2B4O7·10H2O, addition distilled water, heating for dissolving,
Obtain solution A；
Weigh sodium lauryl sulphate, ethylene glycol, add distilled water, heating for dissolving, obtain solution B；
Weigh disodium hydrogen phosphate, add distilled water, heating for dissolving, obtain solution C；
Mixing after above-mentioned solution A, solution B, solution C cooling, regulation PH is 7.0, is prepared as the dodecane of 25-35g/L
Base metabisulfite solution.
Preferably, described acid detergent, collocation method is:
Weigh hexadecane trimethyl ammonium bromide, be dissolved in the sulfuric acid solution that mass percent concentration is 0.5%, be prepared into
To hexadecane trimethyl ammonium bromide and the mixed aqueous solution of sulphuric acid.
Preferably, the content of described neutral detergent fiber, assay method is:
Take the residue sample of appropriate sweet sorghum stalk, be weighed as M1, add and take 0.5 times of M1The anhydrous sodium sulfate of quality is anhydrous
Sodium sulfite；Add appropriate neutral detergent and take M1The 1-capryl alcohol of quality, disappears and boils, sucking filtration, and boiling water rinses, and sucking filtration uses acetone
Rinsing sucking filtration, 105 DEG C are dried overnight, and samples weighing is M2。
Preferably, described acid detergent fiber content, assay method is:
Taking the residue sample of appropriate sweet sorghum stalk, samples weighing is M3；Add appropriate acid detergent and add M3Quality
1-capryl alcohol；Disappearing and boil, sucking filtration, boiling water rinses, and sucking filtration uses acetone rinsing sucking filtration；105 DEG C are dried overnight, and samples weighing is M4；Sample
Adding mass percent concentration in product is the sulphuric acid of 68-75%, and stirring in the pasty state, stands；Appropriate distilled water flushing three times, 105
DEG C being dried overnight, after cooling, samples weighing is M5；500 DEG C of ashing 2h, after cooling, samples weighing is M6。
Preferably, described washing fiber, computational methods are:
Neutral detergent fiber W (NDF)=M2/M1100%
Cellular content W1=1-W (NDF)
Acid detergent fiber W (ADF)=M4/M3100%
Hemicellulose W2=W (NDF)-W (ADF)
Cellulose W3=(M4-M5)/M3100%
Acidic cleaning lignin W (ADL)=(M5-M6)/M3100%
Insoluble ash W4=M6/M3100%
Lignin W5=1-W1–W2–W3–W4。
Compared with prior art, the present invention has a following beneficial effect:
The method washing fiber content in the mensuration sweet sorghum stalk residue of the present invention has the advantage that sample is through pre-place
Reason, the impurity of interference measurement is excluded.Laboratory operating procedures is simple, utilizes laboratory standing instrument such as electric furnace and sucking filtration to fill
Put, get final product a measure and calculation and go out neutral detergent fiber content, acid detergent fiber content, acidic cleaning content of lignin, fibre
Dimension cellulose content, hemicellulose level, content of lignin, result is accurately reliable, in biomass lignocellulose component analysis, raw
The fields such as the thing energy have application and reference value.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in the technology of this area
Personnel are further appreciated by the present invention, but limit the present invention the most in any form.It should be pointed out that, the ordinary skill to this area
For personnel, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement.These broadly fall into the present invention
Protection domain.
Test philosophy of the present invention: neutral detergent makes plant cell rupture, cellular content overflows and (includes protein, fat
Fat, the carbohydrate of solubility, the ashy substance of solubility), and cellulose substances such as cellulose, lignin, half fiber
Plain and insoluble ash fraction then remains, mainly the component of cell wall, referred to as neutral detergent fiber.Acid detergent can
To dissolve the content composition in biomass and hemicellulose components, remaining residue is mainly cellulose, hemicellulose and silicon
Hydrochlorate composition, therefore the content of hemicellulose can be by the content of neutral detergent fiber and the difference of acid detergent fiber content
Obtain.The sulphuric acid (sulphuric acid of preferably 72%) of 68-75% can dissolve the cellulose components in acid detergent fiber, therefore fiber
The content of element can be obtained by the percent weight loss before and after the sulfuric acid treatment of calculating 72%.Sample through acidic cleaning and and
After 72% sulfuric acid treatment, remaining composition is mainly lignin and silicates, then after 500 DEG C are ashed, acidic cleaning wood
Quality is escaped by after-flame, and residual components is mainly ash and silicate.Therefore the content of acidic cleaning lignin can be by meter
Calculate the percent weight loss before and after ashing to obtain.Because cellulose, hemicellulose, lignin, cellular content and insoluble ash
Content is divided to be added equal to 1.Therefore the content of lignin can deduct cellulose by 1, hemicellulose, cellular content and can not
Molten content of ashes obtains.
Following example are carried out according to following enforcement step, in reality, neutral detergent, acid detergent, 68-75%
Sulphuric acid (72% sulphuric acid) configuration can be before whole pretreatment, it would however also be possible to employ existing matured product.
Step 1: pre-treatment step, obtains measuring straw residue sample
Step 2: configuration neutral detergent
Step 3: configuration acid detergent
Step 4: configure 72% sulphuric acid
Step 5: weigh straw residue sample, measures neutral detergent fiber
Step 6: weigh straw residue sample, measure other indexs
In following example of the present invention, grinding and sieve, sample can be made homogeneous in pre-treatment step, particle diameter diminishes, side
Experimental implementation continuous after an action of the bowels；
In following example of the present invention, the acting as of distilled water wash in pre-treatment step: remove impurity interference in test；
In following example of the present invention, what in pre-treatment step, drying sealing bag was sealed up for safekeeping act as: avoid straw residue
Rotting, constituent changes；
In following example of the present invention, use 1-capryl alcohol as defoamer, be possible to prevent during sample disappears and boils, with bubble
Foam overflows container, causes error and the mistake of experiment；
In following example of the present invention, use step-wise dissolution and the configuration of reagent, make material dissolve fully, it is to avoid dissolubility
The material different with dissolubility interferes in configuration.
Embodiment one:
1. the pretreatment of sweet sorghum stalk: remove root and leaf after being gathered in the crops by sweet sorghum stalk, be dried process；
Removing root and leaf after being gathered in the crops by sweet sorghum stalk, by chaser of squeezing the juice, extruding obtains the juice of sugar grass
And straw biomass.Straw biomass after carrying out air-drying process, sieve after grinding and obtain the biomass samples of 40-60 mesh.
Then according to the ratio of distilled water and sweet sorghum stalk 10:1 is washed straw three times, remove the sugarcane of residual in stem stalk biomass
The materials such as sugar, glucose and fructose.Then it is dried 24 hours in the baking oven of 60 DEG C, after being cooled to room temperature in exsiccator, uses
Sealing bag seals, in order to avoid the degraded that the materials such as cellulase are to biomass, experiment is caused error.
2. configuration neutral detergent
Described neutral detergent uses the sodium dodecyl sulfate solution of 25-35g/L, the dodecyl sulfur of preferably 30g/L
Acid sodium.
The beaker taking 100ml weighs 18.61g disodiumedetate (Na2EDTA), 6.81g sodium tetraborate
(Na2B4O7·10H2O), distilled water, heating for dissolving are added；Take 500ml beaker and weigh 30g sodium lauryl sulphate, 10ml second two
Alcohol ether, adds distilled water, heating for dissolving；Take 100ml beaker, weigh 11.72g disodium hydrogen phosphate, add distilled water,
Heating for dissolving；Mixing after cooling, regulation PH is 7.0, is settled to 1000ml；
3. configuration acid detergent
Described acid detergent is the mixed aqueous solution of hexadecane trimethyl ammonium bromide and sulphuric acid, preferably 20g/L hexadecane
Trimethylammonium bromide.
Configuration quality concentration 0.5% sulfuric acid solution, takes 250ml beaker, adds suitable quantity of water, is placed on balance clearing, weighs
49g concentrated sulphuric acid, adds along glass wall of cup, is stirred continuously, is settled to 1000ml；Take 1000ml beaker, weigh 20g hexadecane front three
Base ammonium bromide；Dissolve under room temperature.
4. configuration 72% sulphuric acid: cleaning, drying 1000ml graduated cylinder, reagent bottle, volumetric flask；Graduated cylinder weighs 652ml concentrated sulphuric acid；
It is dissolved in the distilled water of 300ml, cooling；Under room temperature, 1000ml volumetric flask constant volume；
5. take appropriate amount of sample (through pretreated sweet sorghum stalk residue), be weighed as 0.551g (M1), add 0.5g
Anhydrous sodium sulfite；Add appropriate neutral detergent and 1ml 1-capryl alcohol (defoamer)；Electric furnace disappears and boils 60min；Take out
Filter, boiling water rinses, and sucking filtration, with 20ml acetone rinsing sucking filtration；105 DEG C are dried overnight, and samples weighing is 0.3675g (M2)；
6. taking appropriate amount of sample (through pretreated sweet sorghum stalk residue), samples weighing is 1.0488g (M3)；Add
Appropriate acid detergent and 1ml 1-capryl alcohol (defoamer)；Electric furnace disappears and boils 60min；Sucking filtration, boiling water rinses, sucking filtration,
With 20ml acetone rinsing sucking filtration；105 DEG C are dried overnight, and samples weighing is 0.4681g (M4)；Sample adds 72% sulphuric acid, stirs
Mix in the pasty state, stand 3h；Appropriate distilled water flushing, 105 DEG C are dried overnight, and after cooling, samples weighing is 0.0862g (M5)；500
DEG C ashing 2h, after cooling, samples weighing is 0.0085g (M6)；
7. washing fiber computational methods are:
Neutral detergent fiber W (NDF)=M2/M1100%=0.3675/0.551 100%=66.70%
Cellular content W1=1-W (NDF)=1-66.70%=33.3%
Acid detergent fiber W (ADF)=M4/M3100%=0.4681/1.0488 100%=44.63%
Hemicellulose W2=W (NDF)-W (ADF)=22.07%
Cellulose W3=(M4-M5)/M3100%=(0.4681-0.0862)/1.0488 100%=36.41%
Acidic cleaning lignin W (ADL)=(M5-M6)/M3100%=(0.0862-0.0085)/1.0488 100%
=7.41%
Insoluble ash W4=M6/M3100%=0.0085/1.0488 100%=0.81%
Lignin W5=1-W1–W2–W3–W4=1 33.3% 22.07% 36.41% 0.81%=7.41%
Embodiment two:
1. the pretreatment of sweet sorghum stalk, obtains sweet sorghum stalk residue after pretreatment；
Removing root and leaf after being gathered in the crops by sweet sorghum stalk, by chaser of squeezing the juice, extruding obtains the juice of sugar grass
And straw biomass.Straw biomass after carrying out air-drying process, sieve after grinding and obtain the biomass samples of 40-60 mesh.
Then according to the ratio of distilled water and sweet sorghum stalk 10:1 is washed straw three times, remove the sugarcane of residual in stem stalk biomass
The materials such as sugar, glucose and fructose.Then it is dried 24 hours in the baking oven of 60 DEG C, after being cooled to room temperature in exsiccator, uses
Sealing bag seals, in order to avoid the materials such as cellulase are to biomass degradation, experiment is caused error.
2. configuration neutral detergent (sodium lauryl sulphate of 30g/L) collocation method is: the beaker taking 100ml weighs
18.61g disodiumedetate (Na2EDTA), 6.81g sodium tetraborate (Na2B4O7·10H2O), add distilled water, add thermosol
Solve；Take 500ml beaker and weigh 30g sodium lauryl sulphate, 10ml ethylene glycol, add distilled water, heating for dissolving；Take
100ml beaker, weighs 11.72g disodium hydrogen phosphate, adds distilled water, heating for dissolving；Mixing after cooling, regulation PH is
7.0, it is settled to 1000ml；
3. configuration acid detergent (20g/L hexadecane trimethyl ammonium bromide) collocation method is: configure 0.5% sulphuric acid molten
Liquid, takes 250ml beaker, adds suitable quantity of water, is placed on balance clearing, weighs 49g concentrated sulphuric acid, adds along glass wall of cup, constantly stirs
Mix, be settled to 1000ml；Take 1000ml beaker, weigh 20g hexadecane trimethyl ammonium bromide；Dissolve under room temperature；
4. configuration 72% sulphuric acid collocation method is: cleaning, drying 1000ml graduated cylinder, reagent bottle, volumetric flask；Graduated cylinder weighs
652ml concentrated sulphuric acid；It is dissolved in the distilled water of 300ml, cooling；Under room temperature, 1000ml volumetric flask constant volume；
5. take appropriate amount of sample (sweet sorghum stalk residue), be weighed as 0.548g (M1), add 0.5g anhydrous sodium sulfite；Add
Enter appropriate neutral detergent and 1ml 1-capryl alcohol (defoamer)；Electric furnace disappears and boils 60min；Sucking filtration, boiling water rinses, takes out
Filter, with 20ml acetone rinsing sucking filtration；105 DEG C are dried overnight, and samples weighing is 0.3638g (M2)；
6. taking appropriate amount of sample (sweet sorghum stalk residue), samples weighing is 1.0099g (M3)；Add appropriate acid detergent
With 1ml 1-capryl alcohol (defoamer)；Electric furnace disappears and boils 60min；Sucking filtration, boiling water rinses, and sucking filtration uses 20ml acetone rinsing
Sucking filtration；105 DEG C are dried overnight, and samples weighing is 0.4504g (M4)；Adding 72% sulphuric acid in sample, stirring in the pasty state, stands
3h；Cold water flush, 105 DEG C are dried overnight, and after cooling, samples weighing is 0.0814g (M5)；500 DEG C of ashing 2h, sample after cooling
It is weighed as 0.0073g (M6)；
7. washing fiber computational methods are:
Neutral detergent fiber W (NDF)=M2/M1100%=0.3638/0.548 100%=66.39%
Cellular content W1=1-W (NDF)=1-66.39%=33.61%
Acid detergent fiber W (ADF)=M4/M3100%=0.4504/1.0099 100%=44.60%
Hemicellulose W2=W (NDF)-W (ADF)=21.79%
Cellulose W3=(M4-M5)/M3100%=(0.4504-0.0814)/1.0099 100%=36.54%
Acidic cleaning lignin W (ADL)=(M5-M6)/M3100%=(0.0814-0.0073)/1.0099 100%
=7.34%
Insoluble ash W4=M6/M3100%=0.0073/1.0099 100%=0.72%
Lignin W5=1-W1–W2–W3–W4=1 33.61% 21.79% 36.54% 0.72%=7.34%
To sum up, the present invention utilizes laboratory standing instrument such as electric furnace, Suction filtration device etc., can once measure and can count
Calculate neutral detergent fiber content, acid detergent fiber content, acidic cleaning content of lignin, content of cellulose, hemicellulose
Content, content of lignin.Whole method step is simple, and result is the most reliable.The present invention studies in sweet sorghum stalk residue and washs
The content of fiber, the utilization for its resource provides data and theories integration.
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformation or amendment within the scope of the claims, this not shadow
Ring the flesh and blood of the present invention.
Claims (7)
1. one kind measures the method washing fiber content in sweet sorghum stalk residue, it is characterised in that: comprise the steps:
The pretreatment of S1: sweet sorghum stalk: remove root, fringe and foliage portion after being gathered in the crops by sweet sorghum stalk, be dried process,
The residue sample of sweet sorghum stalk, for follow-up mensuration；
S2: weigh the residue sample of appropriate described sweet sorghum stalk, disappear with neutral detergent and boil, sucking filtration with acetone rinsing, dry
The content of neutral detergent fiber is obtained after dry；Described neutral detergent is the sodium dodecyl sulfate solution of 25-35g/L；
S3: again weigh the residue sample of described sweet sorghum stalk, disappear with acid detergent and boil, sucking filtration with acetone rinsing, dry
Obtain solid after dry, be calculated acid detergent fiber content；Described acid detergent is hexadecane trimethyl ammonium bromide and sulfur
The mixed aqueous solution of acid；
S4: in the solid that S3 obtains, adding mass percent concentration is the sulphuric acid of 68-75%, and stirring in the pasty state, stands, and steams
Distilled water is dried after rinsing and weighs；
S5: the solid arrived of S4, carries out ashing process, weighs, be calculated acidic cleaning content of lignin after ashing cooling,
Content of cellulose, hemicellulose level, content of lignin.
The method washing fiber content in mensuration sweet sorghum stalk residue the most according to claim 1, it is characterised in that: institute
The S1 stated, step is:
S101: removing root, tassel and leaf after being gathered in the crops by sweet sorghum stalk, by chaser of squeezing the juice, extruding obtains sugar grass
Juice and sweet sorghum stalk residue；
S102: the sweet sorghum stalk residue obtained by S101 air-dries after processing, and sieves and obtain the biomass sample of 40-60 mesh after grinding
Product；
S103: the biomass samples obtained by S102, according to distilled water and biomass samples mass ratio 5-50, biomass sample
Product wash three times, remove sucrose, glucose and the fructose material of residual in biomass samples；
S104: the process carrying out the material after S103 processes, is dried 24 hours in the baking oven of 60 DEG C, cold in exsiccator
But to after room temperature, seal up for safekeeping with sealing bag, in order to avoid the degraded that the materials such as cellulase are to biomass samples, result is caused error.
The method washing fiber content in mensuration sweet sorghum stalk residue the most according to claim 1, it is characterised in that: institute
Stating neutral detergent, collocation method is:
Weigh disodiumedetate Na2EDTA, sodium tetraborate Na2B4O7·10H2O, adds distilled water, heating for dissolving, obtains
Solution A；
Weigh sodium lauryl sulphate, ethylene glycol, add distilled water, heating for dissolving, obtain solution B；
Weigh disodium hydrogen phosphate, add distilled water, heating for dissolving, obtain solution C；
Mixing after above-mentioned solution A, solution B, solution C cooling, regulation PH is 7.0, is prepared as the dodecyl sulfur of 25-35g/L
Acid sodium solution.
The method washing fiber content in mensuration sweet sorghum stalk residue the most according to claim 1, it is characterised in that institute
The acid detergent stated, collocation method is:
Weigh hexadecane trimethyl ammonium bromide, be dissolved in the sulfuric acid solution that mass percent concentration is 0.5%, prepare ten
Six alkane trimethylammonium bromides and the mixed aqueous solution of sulphuric acid.
5., according to the method washing fiber content in the mensuration sweet sorghum stalk residue described in any one of claim 1-4, it is special
Levy and be, the content of described neutral detergent fiber, assay method is:
Take the residue sample of appropriate sweet sorghum stalk, be weighed as M1, add 0.5 times of M1The anhydrous sodium sulfate anhydrous sodium sulphuric acid of quality
Sodium；Add appropriate neutral detergent and M1The 1-capryl alcohol of quality, disappears and boils, sucking filtration, and boiling water rinses, and sucking filtration uses acetone rinsing sucking filtration,
105 DEG C are dried overnight, and samples weighing is M2。
The method washing fiber content in mensuration sweet sorghum stalk residue the most according to claim 5, it is characterised in that institute
Stating acid detergent fiber content, assay method is:
Taking the residue sample of appropriate sweet sorghum stalk, samples weighing is M3；Add appropriate acid detergent and add M3The 1-of quality
Capryl alcohol；Disappearing and boil, sucking filtration, boiling water rinses, and sucking filtration uses acetone rinsing sucking filtration；105 DEG C are dried overnight, and samples weighing is M4；In sample
Adding mass percent concentration is the sulphuric acid of 68-75%, and stirring in the pasty state, stands；Appropriate distilled water flushing three times, does for 105 DEG C
Dry overnight, after cooling, samples weighing is M5；500 DEG C of ashing 2h, after cooling, samples weighing is M6。
The method washing fiber content in mensuration sweet sorghum stalk residue the most according to claim 6, it is characterised in that institute
The washing fiber stated, computational methods are:
Neutral detergent fiber W (NDF)=M2/M1100%
Cellular content W1=1-W (NDF)
Acid detergent fiber W (ADF)=M4/M3100%
Hemicellulose W2=W (NDF)-W (ADF)
Cellulose W3=(M4-M5)/M3100%
Acidic cleaning lignin W (ADL)=(M5-M6)/M3100%
Insoluble ash W4=M6/M3100%
Lignin W5=1-W1–W2–W3–W4。
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| CN108020483A (en) * | 2018-01-25 | 2018-05-11 | 杭州神农牧业有限公司 | Neutral detergent fiber measurement device |
| CN108254327A (en) * | 2018-01-24 | 2018-07-06 | 上海理工大学 | The improved method of lignocellulose biomass component analysis |
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| CN111122726B (en) * | 2019-12-20 | 2024-02-23 | 上海市农业科学院 | Edible fungus culture medium lignocellulose component testing equipment and method thereof |
| CN113109295A (en) * | 2021-03-31 | 2021-07-13 | 杭州融凯盛科技有限公司 | Method for determining waste residue composition in Lyocell production system |
| CN115308079A (en) * | 2022-08-26 | 2022-11-08 | 攀钢集团重庆钒钛科技有限公司 | Characterization method for acidolysis rate of titanium concentrate in laboratory |
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