CN109490136A - A kind of acid and neutral detergent fiber content method in quick measurement feed - Google Patents
A kind of acid and neutral detergent fiber content method in quick measurement feed Download PDFInfo
- Publication number
- CN109490136A CN109490136A CN201910010577.3A CN201910010577A CN109490136A CN 109490136 A CN109490136 A CN 109490136A CN 201910010577 A CN201910010577 A CN 201910010577A CN 109490136 A CN109490136 A CN 109490136A
- Authority
- CN
- China
- Prior art keywords
- feed
- filter bag
- fiber filter
- detergent fiber
- neutral detergent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
- G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of acid and neutral detergent fiber content methods in quickly measurement feed, comprising: the pre-treatment of Feed Sample；It prepares dry matter Feed Sample and records dry matter content；Fiber filter bag is dry in drier, it weighs after cooling；Feed Sample is fitted into the fiber filter bag of known quality and number；Fiber filter bag is put into nylon wire, concussion in pectinase solution is immersed in after bandaging to handle, it is immersed in alpha-amylase solution concussion processing again, it is immersed in Thermostable α-Amylase neutral detergent solution and washs, first rinsed with cold water, it is rinsed again with the boiling water of flowing, acetone submergence is taken out and submerged again with new acetone；It is weighed after drying to constant weight；Calculate the neutral detergent fiber content in feed；Fiber filter bag is washed under slight boiling condition with alpha-amylase acidic cleaning liquid, is rinsed with cold water, and the boiling water of flowing rinses；After being submerged in acetone, takes out and submerged again with new acetone；It is weighed after drying to constant weight；Calculate the acid detergent fiber content in feed.
Description
Technical field
The present invention relates to acid in quickly measurement feed a kind of in Animal nutrition laboratory, feed industry and related fields and
The method of neutral detergent fiber content.
Background technique
Neutral detergent fiber (Neutral detergent fiber, NDF) mainly includes hemicellulose and acidic cleaning
Fiber (ingredients such as cellulose and lignin), is made of insoluble non-starch polysaccharide and lignin, is that one kind can be calibrated
Really reflect the nutriment of feed fibre actual content.In cud, due to its relative to non-fiber class carbohydrate (such as
Starch, sucrose etc.) fermenting speed is slow, it is thus possible to and the growth metabolism for being continuously ruminant and rumen microorganism provides energy
Amount.But excessively high NDF can then generate negative effect to dry matter intake and breeding performonce fo animals, thus Accurate Determining its in feed
In content have great importance.
Currently, for neutral detergent fiber and acid detergent fiber measurement mainly using being analyzed by normal form based on
And a variety of different measuring methods developed, it is more commonly mainly Fibre Bag washing method and Gooch crucible suction method.By
It is cumbersome in Gooch crucible suction method program, measurement sample size it is few, therefore high-volume measurement Feed Sample when it is at high cost, time-consuming.
Fiber filter bag method has the characteristics that simply and easily the batch that can carry out NDF ingredient quickly measures.This technology is main at present
Measurement applied to NDF and ADF in feed and food.It is cooperated within 1993, is developed by Cornell Univ USA and Canada
ANKOM analysis analyzer is simultaneously patented, and the product and technology adopted by many national governments, research institution in the world
With, but the instrument and equipment price as used by ANKOM filter bag technology is more expensive, and every batch of measurement quantity only has 24, therefore
Also it is unable to satisfy the demand that high-volume measures Feed Sample.
In view of the above-mentioned problems, a kind of method for quickly, effectively measuring feed neutrality and acid detergent fiber of the present invention.
The method is first pre-processed by biological enzyme (such as amylase, pectase), is then washed using neutral detergent and acidity
Wash agent washed, degreasing, drying weighing, the NDF and ADF that high-volume Feed Sample can be completed effectively measure.The method
Analyzer is analyzed without using crude fibre analyzer and ANKOM, only operation need to can be completed by common beaker and electronics universal furnace,
Have the characteristics that simple, easily and fast high with accuracy, the batch that can carry out NDF and ADF ingredient effectively measures, thus
Practicability and application are had more in laboratory, feed industry and its related fields.
Summary of the invention
Simple, quick, effectively the neutrality in feed can be measured the technical problem to be solved in the present invention is to provide one kind to wash
The method for washing fiber content.
The invention solves another technical problem be to provide one kind can it is simple, quickly, effectively in measurement feed
The method of acid detergent fiber content.
To achieve the above object, the invention provides the following technical scheme:
A kind of acid and neutral detergent fiber content method in quick measurement feed, comprising the following steps:
(1) it the pre-treatment of Feed Sample: is processed according to the characteristic of the Feed Sample of acquisition, then uses feed meal
Broken machine crushes and crosses 1mm sieve；
(2) by step (1) treated Feed Sample, drying to constant weight, prepares dry matter Feed Sample and records dry matter
Content.
(3) it by fiber filter bag in drier, after 105 DEG C are dried 2 hours, weighs after cooling, is denoted as m0g；
(4) 0.45-0.55g Feed Sample is fitted into the fiber filter bag of known quality and number, gross weight is denoted as m1G, so
It is completely sealed with sealing machine in two sides afterwards；
(5) the fiber filter bag that step (4) obtains is put into nylon wire, is immersed in after bandaging in pectinase solution at concussion
Reason；
(6) by step (5) treated fiber filter bag is immersed in again Thermostable α-Amylase solution concussion processing；
(7) by step (6), treated that fiber filter bag is immersed in Thermostable α-Amylase neutral detergent solution washs；
(8) step (7) treated fiber filter bag is first rinsed 30 minutes with cold water, then with 10 points of the boiling water flushing of flowing
Clock；
(9) step (8) treated fiber filter bag is first submerged 10 minutes with acetone, takes out and submerges 10 with new acetone again
Minute；
(10) by step (9), treated weighs after fiber filter bag dries to constant weight, and is denoted as m2g；Calculate the neutrality in feed
Wash fiber content: neutral detergent fiber (%)=100% × (m2-m0)/(m1-m0)。
(11) by step (10), treated that the fiber filter bag Thermostable α-Amylase acidic cleaning liquid of 10000u/L exists
It is washed 70 minutes under slight boiling condition；
(12) after rinsing step (11) treated fiber filter bag 30 minutes with cold water, 10 points are rinsed with the boiling water of flowing
Clock；
(13) by step (12) treated fiber filter bag submerges in acetone after ten minutes, take out again with new acetone leaching
Not 10 minutes；
(14) by step (13), treated weighs after fiber filter bag dries to constant weight, and gross weight is denoted as m3g；It calculates in feed
Acid detergent fiber content: acid detergent fiber (%)=100% × (m3-m0)/(m1-m0)。
It in step (1), is processed and is specifically included according to the characteristic of the Feed Sample of acquisition, for moisture content height
Feed, first dried, measure its moisture；For longer or thicker roughage, is first cut short or cut carefully, then crushed
1mm sieve.
In step (3), 105 DEG C of drying temperature, drying time 2 hours.
In step (5), the aperture of nylon wire is 1mm.
In step (5), the temperature of pectinase solution is 50 DEG C, and the concussion processing time is 8 hours.
In step (6), the temperature of Thermostable α-Amylase solution is 95 DEG C, the concussion processing time 16 hours.
In step (7), washing is slightly boiled washing 70 minutes in Thermostable α-Amylase washing solution.
In step (7), washing is washed 70 minutes during continuous submergence stirring.
In step (8), boiling water washing is rinsed with the boiling water of flowing, and the time is 10 minutes.
The beneficial effects of the present invention are:
(1) present invention is pre-processed by pectase and Thermostable α-Amylase first, can be effectively removed in influence
Property washing fiber and acid detergent fiber measurement the great influence factor-starch and pectin, improve the accuracy of measuring method；
(2) enzymatic treatment and washing process can not only realize the batch operation of Feed Sample by nylon wire, while can
Relative error is reduced, accuracy is improved；
(3) it is rinsed in washing process by the cold water of flowing and boiling water, cleaning solution and sulfuric acid solution can be effectively removed
It destroys, ensure the integrality of fiber filter bag and saves cost；
(4) it is submerged by acetone, it being capable of effectively degreasing and decoloration；
(5) continuous mode is by common beaker and 2 six multi pack electronics universal furnaces, simple and crude condition can it is simple, batch
Measure to amount (a batch can measure 600 Feed Samples simultaneously) content of neutral detergent fiber and acid detergent fiber in feed.
Thus, the present invention, which is capable of providing one kind, simple, quick, effective to measure neutral detergent fiber and acidity in feed
The method for washing fiber content.
Specific embodiment
1, material
(1) instrument and equipment:
Feed grinder (JFSD-100- Π type, Jiading Grain and Oil Instrument Co., Ltd., Shanghai)；Fiber filter bag (Chinese agriculture
University beef cattle center)；Round-trip constant temperature oscillator (WHY-2 type, Jin Cheng Guo Sheng test apparatus factory, Community of Jin Tan County city)；Electric heating constant temperature drum
Wind drying box (DGG-9240B type, Shanghai is gloomy reliable to test Instrument Ltd.)；Six multi pack electronics universal furnace (Shanghai section perseverance industry
Development Co., Ltd)；Sealing machine (AIE-200 type, AMERICAN INT ' NL ELECTRIC)；Silica gel drier (diameter
400mm, Shenzhen are auspicious prosperous up to Hua Bo Instrument Ltd.)；5000mL glass beaker (safe experiment equipment factory, Haimen City)；Asbestos
Net (diameter 15cm, Dongguan City Dong Wang Hua Bo Instrument Ltd.)；1000mL graduated cylinder, thermos flask, kettle etc..
(2) reagent:
Thermostable α-Amylase (enzyme activity 10000u/mL, Wuxi Xue Mei enzyme preparation Co., Ltd)；Pectase (enzyme activity
300000u/mL, Tai'an letter are got profit bioengineering Co., Ltd)；
30g/L sodium dodecyl sulfate solution: 18.6g disodium ethylene diamine tetraacetate (Na is weighed2EDTA, chemistry are pure) and
6.81g sodium tetraborate (Na2B4O7·10H2O), and it is put into 1000mL beaker, after adding appropriate distilled water to dissolve by heating, adds
30g lauryl sodium sulfate and 10mL ethylene glycol ethyl ether.It weighs 4.56g Anhydrous Disodium Phosphate to be placed in another beaker, add few
Amount distilled water pours into first beaker after dissolving by heating cooling, is diluted to 1000mL.
0.5mol/L sulfuric acid solution: weighing the 27.87mL concentrated sulfuric acid, is slowly added into the 1000mL capacity for having been loaded with 500mL water
In bottle, constant volume after cooling.
Acidic cleaning agent solution (2g/L cetyl trimethylammonium bromide solution): 20g cetyl trimethyl bromine is weighed
Change ammonium, is dissolved in 1000mL 0.5mol/L sulfuric acid solution.
2, embodiment
A kind of acid and neutral detergent fiber content method in quick measurement feed, comprising the following steps:
(1) it the pre-treatment of Feed Sample: is processed according to the characteristic of the Feed Sample of acquisition, then uses feed meal
Broken machine crushes and crosses 1mm sieve；It is processed and is specifically included according to the characteristic of the Feed Sample of acquisition, for moisture content height
Feed, such as whole corn silage first dried, its moisture is measured；For longer or thicker roughage, such as sheep's hay, first
It is cut short or is cut and is thin；
(2) by step (1) treated Feed Sample, drying to constant weight, prepares dry matter Feed Sample and records dry matter
Content.
(3) it by fiber filter bag in drier, after 105 DEG C are dried 2 hours, weighs after cooling, is denoted as m0g；
(4) 0.45-0.55g Feed Sample is fitted into the fiber filter bag of known quality and number, gross weight is denoted as m1G, so
It is completely sealed with sealing machine in two sides afterwards；
(5) the fiber filter bag that step (4) obtains is put into the nylon wire that aperture is 1mm, is immersed in after bandaging
Concussion processing 8 hours in 300000u/L pectinase solution, wherein the temperature of pectinase solution is 50 DEG C；
(6) the Thermostable α-Amylase solution of step (5) treated fiber filter bag is immersed in again 20000u/L is shaken
Processing 16 hours, wherein the temperature of alpha-amylase solution is 95 DEG C；
(7) by the Thermostable α-Amylase neutral detergent solution of step (6) treated fiber filter bag is immersed in 10000u/L
In, it is washed 70 minutes under slight boiling condition；
(8) step (7) treated fiber filter bag is first rinsed 30 minutes with cold water, then with 10 points of the boiling water flushing of flowing
Clock；
(9) step (8) treated fiber filter bag is first submerged 10 minutes with acetone, takes out and submerges 10 with new acetone again
Minute；
(10) by step (9), treated weighs after fiber filter bag dries to constant weight, and is denoted as m2g；Calculate the neutrality in feed
Wash fiber content: neutral detergent fiber (%)=100% × (m2-m0)/(m1-m0)。
(11) by step (10), treated that the fiber filter bag Thermostable α-Amylase acidic cleaning liquid of 10000u/L exists
It is washed 70 minutes under slight boiling condition；
(12) after rinsing step (11) treated fiber filter bag 30 minutes with cold water, 10 points are rinsed with the boiling water of flowing
Clock；
(13) by step (12) treated fiber filter bag submerges in acetone after ten minutes, take out again with new acetone leaching
Not 10 minutes；
(14) by step (13), treated weighs after fiber filter bag dries to constant weight, and gross weight is denoted as m3g；It calculates in feed
Acid detergent fiber content: acid detergent fiber (%)=100% × (m3-m0)/(m1-m0)。
Embodiment 1:
Using the method for the invention, by 10 parts from Anhui Province Fengyang County, Guzhen County, Wuhe County, Fengtai County, Linquan County,
The corn feed sample that Suixi County, Lixin County, Wuwei County, Guangde County, Feidong County acquire crushes using pulverizer and crosses 1mm sieve,
Drying to constant weight, measures its neutral detergent fiber and acid detergent fiber content after measuring its dry matter content respectively.
Embodiment 2:
Using the method for the invention, by 10 parts from Anhui Province Fengyang County, Guzhen County, Wuhe County, Fengtai County, Linquan County,
The bean-dregs feed sample that Suixi County, Lixin County, Wuwei County, Guangde County, Feidong County acquire crushes using pulverizer and crosses 1mm sieve,
Drying to constant weight, measures its neutral detergent fiber and acid detergent fiber content after measuring its dry matter content.
Embodiment 3: utilizing the method for the invention, will be from Anhui Province Fengyang County, Wuhe County, Huoshan County, Suixi County, Guzhen
5 parts of imported from America clovers, the 2 parts of domestic clovers, 3 parts of domestic sheep of well-known cattle farm, beef cattle and the acquisition of mutton sheep farm in county
Grass, 5 parts of ensiling whole-plant corns, 5 parts of corn stovers crush using pulverizer and cross 1mm sieve, and wherein ensiling whole-plant corn is first dried
After crush, drying to constant weight, measures its neutral detergent fiber and acid detergent fiber content respectively after measuring its dry matter content.
3, test example
Test example 1: using corn as the neutral detergent fiber and acid detergent fiber percentage in the energy feed raw material of representative
Assay.
Selection 10 parts (Corn, C1-C10) corn feed sample for being acquired from Anhui Province various regions, crushed, be prepared into it is dry
After material sample, each corn sample does 15 in parallel, measures its neutral detergent fiber and acidity according to the method for the invention
Wash fiber content.
Each corn sample does 5 parallel, reference National Standard Method (GB/T 20806-2006) and the People's Republic of China (PRC) simultaneously
It is former to measure corn feed respectively for the measuring method of acid detergent fiber in agricultural industry criteria (NY/T 1459-2007)-feed
The content of neutral detergent fiber and acid detergent fiber in material, and measurement result is compared to analysis as a control group.
Test result is as follows:
Neutral detergent fiber percentage composition [means standard deviation (coefficient of variation)] measurement result is analyzed in 1 corn of table
Note: different letters indicate significant difference (P < 0.05) on the right side of the data between not going together, the same below.
As shown in Table 1, using the method for the invention measurement corn feed sample in neutral detergent fiber content with
Without significant between the neutral detergent fiber content in corn feed sample measured referring to National Standard Method (GB/T 20806-2006)
Difference.But when measuring the neutral detergent fiber content in corn feed sample referring to National Standard Method (GB/T 20806-2006),
Coefficient of variation variation range in group is 0.90%~5.21%；And utilize the corn feed sample of the method for the invention measurement
In neutral detergent fiber content when, the coefficient of variation variation range in group is only 0.15%~1.40%.The above results show
The neutral detergent fiber content in corn feed sample can quickly, be accurately and precisely measured using the method for the invention.
As shown in Table 2, using the method for the invention measurement corn feed sample in acid detergent fiber content with
It is carried out referring to the measurement of acid detergent fiber in People's Republic of China's agricultural industry criteria (NY/T 1459-2007)-feed
It is not significantly different between acid detergent fiber content in the corn feed sample of measuring method measurement.But referring to China
The measurement of acid detergent fiber is measured method in people's republic's agricultural industry criteria (NY/T 1459-2007)-feed
When measuring the acid detergent fiber content in corn feed sample, the coefficient of variation variation range in group is 2.12%~
6.41%；And change when utilizing the acid detergent fiber content in the corn feed sample of the method for the invention measurement, in group
Different index variation range is only 0.52%~2.91%.The above results show can quickly, accurately using the method for the invention
Accurately measure the acid detergent fiber content in corn feed sample.
Acid detergent fiber percentage composition [means standard deviation (coefficient of variation)] measurement result is analyzed in 2 corn of table
Test example 2: using dregs of beans as the neutral detergent fiber and acid detergent fiber percentage in the energy feed raw material of representative
Assay.
It selects 10 parts (Soybean meal, SM1-SM10) from Anhui Province Fengyang County, Guzhen County, Wuhe County, Fengtai County, face
The bean-dregs feed sample that spring county, Suixi County, Lixin County, Wuwei County, Guangde County, Feidong County acquire uses pulverizer crushing and mistake
1mm sieve, drying to constant weight, and after measuring its dry matter content, each dregs of beans sample does 15 in parallel, according to the method for the invention
Its neutral detergent fiber and acid detergent fiber content are measured respectively.
Each dregs of beans sample does 5 parallel, reference National Standard Method (GB/T 20806-2006) and the People's Republic of China (PRC) simultaneously
It is former to measure bean-dregs feed respectively for the measuring method of acid detergent fiber in agricultural industry criteria (NY/T 1459-2007)-feed
The content of neutral detergent fiber and acid detergent fiber in material, and measurement result is compared to analysis as a control group.
Test result is as follows:
Neutral detergent fiber percentage composition [means standard deviation (coefficient of variation)] measurement result is analyzed in 3 dregs of beans of table
As shown in Table 3, using the method for the invention measurement bean-dregs feed sample in neutral detergent fiber content with
Without significant between the neutral detergent fiber content in bean-dregs feed sample measured referring to National Standard Method (GB/T 20806-2006)
Difference.But when measuring the neutral detergent fiber content in bean-dregs feed sample referring to National Standard Method (GB/T 20806-2006),
Coefficient of variation variation range in group is 0.65%~2.22%；And utilize the bean-dregs feed sample of the method for the invention measurement
In neutral detergent fiber content when, the coefficient of variation variation range in group is only 0.30%~2.21%.The above results show
The neutral detergent fiber content in bean-dregs feed sample can quickly, be accurately and precisely measured using the method for the invention.
As shown in Table 4, using the method for the invention measurement bean-dregs feed sample in acid detergent fiber content with
Referring to the measuring method of acid detergent fiber in People's Republic of China's agricultural industry criteria (NY/T 1459-2007)-feed
It is not significantly different between acid detergent fiber content in the bean-dregs feed sample of measurement.But referring to Chinese people's republicanism
The measuring method of acid detergent fiber measures bean-dregs feed sample in state's agricultural industry criteria (NY/T 1459-2007)-feed
In acid detergent fiber content when, the coefficient of variation variation range in group is 2.12%~6.41%；And utilize institute of the present invention
When stating the acid detergent fiber content in the bean-dregs feed sample of method measurement, the coefficient of variation variation range in group is only
0.39%~2.91%.The above results show quickly, accurately and precisely measure dregs of beans feeding using the method for the invention
Expect the acid detergent fiber content in sample.
Acid detergent fiber percentage composition [means standard deviation (coefficient of variation)] measurement result is analyzed in 4 dregs of beans of table
Test example 3: using alfalfa hay, sheep's hay, whole corn silage and corn stover as in the roughage raw material of representative
Neutral detergent fiber and the measurement of acid detergent fiber percentage composition.
It, will be from knowing in Anhui Province Fengyang County, Wuhe County, Huoshan County, Suixi County, Guzhen County using the method for the invention
Name cattle farm, 5 parts of imported from America clovers (Imported Alfalfa, IA1-5) of beef cattle and the acquisition of mutton sheep farm, 2 parts of states
Produce clover (Domestic Alfalfa, DA1-2), 3 parts of domestic sheep's hays (Chinese wild grass hay, CWGH1-3), 5
Part ensiling whole-plant corn (Total corn silage, TCS1-5) and 5 parts of corn stovers (Corn stover, CS1-5) use
Pulverizer crushes and crosses 1mm sieve (ensiling whole-plant corn is first dried to be crushed afterwards), and drying to constant weight, after measuring its dry matter content,
Each Feed Sample does 15 in parallel, measures its neutral detergent fiber and acid detergent fiber respectively according to the method for the invention
Content.
Each Feed Sample does 5 parallel, reference National Standard Method (GB/T 20806-2006) and the People's Republic of China (PRC) simultaneously
The measuring method of acid detergent fiber in agricultural industry criteria (NY/T 1459-2007)-feed measures in feedstuff respectively
The content of neutral detergent fiber and acid detergent fiber, and measurement result is compared to analysis as a control group.
Test result is as follows:
Neutral detergent fiber and acid detergent fiber content [means standard deviation (coefficient of variation)] are surveyed in 5 alfalfa hay of table
Determine interpretation of result
As shown in Table 5, the neutral detergent fiber and acid detergent fiber in the clover of the method for the invention measurement are utilized
Content with referring to National Standard Method (GB/T 20806-2006) and People's Republic of China's agricultural industry criteria (NY/T 1459-
2007) it is not significantly different between the result of measuring method measurement.Moreover, neutral detergent fiber of the present invention and acidity are washed
Fiber content measuring method is washed, the coefficient of variation in group is respectively less than 2.0%.
The above results show quickly, accurately and precisely measure in alfalfa hay using the method for the invention
Neutral detergent fiber and acid detergent fiber content.
Neutral detergent fiber and acid detergent fiber content [means standard deviation (coefficient of variation)] measurement knot in 6 sheep's hay of table
Fruit analysis
Neutral detergent fiber and acid detergent fiber content [means standard deviation (variation lines in 7 whole corn silage of table
Number)] measurement result analysis
Neutral detergent fiber and acid detergent fiber content [means standard deviation (coefficient of variation)] are surveyed in 8 corn stover of table
Determine interpretation of result
By table 6-8 it is found that using in the sheep's hay of the method for the invention measurement, whole corn silage and corn stover
Property washing fiber and acid detergent fiber content with it is agriculture referring to National Standard Method (GB/T 20806-2006) and the People's Republic of China (PRC)
It is not significantly different between the result of professional standard (NY/T 1459-2007) measuring method measurement.Moreover, of the present invention
Neutral detergent fiber and acid detergent fiber content assaying method, the coefficient of variation in group are respectively less than 1.0%.
The above results show quickly, accurately and precisely measure the neutrality in feed using the method for the invention
Wash fiber and acid detergent fiber content.
The embodiments of the present invention described above are not intended to limit the scope of the present invention.It is any in the present invention
Spirit and principle within made modifications, equivalent substitutions and improvements etc., should be included in claim protection model of the invention
Within enclosing.
Claims (7)
1. a kind of acid and neutral detergent fiber content method in quickly measurement feed, comprising the following steps:
(1) it the pre-treatment of Feed Sample: is processed according to the characteristic of the Feed Sample of acquisition, then uses feed grinder
It crushes and crosses 1mm sieve；
(2) by step (1) treated Feed Sample, drying to constant weight, prepares dry matter Feed Sample and records dry matter and contains
Amount；
(3) it by fiber filter bag in drier, after 105 DEG C are dried 2 hours, weighs after cooling, is denoted as m0g；
(4) 0.45-0.55g Feed Sample is fitted into the fiber filter bag of known quality and number, gross weight is denoted as m1G, then with envelope
Mouth machine is completely sealed in two sides；
(5) the fiber filter bag that step (4) obtains is put into nylon wire, concussion in pectinase solution is immersed in after bandaging and is handled；
(6) by step (5) treated fiber filter bag is immersed in again Thermostable α-Amylase solution concussion processing；
(7) by step (6), treated that fiber filter bag is immersed in Thermostable α-Amylase neutral detergent solution washs；
(8) step (7) treated fiber filter bag is first rinsed 30 minutes with cold water, then with boiling water flushing 10 minutes of flowing；
(9) step (8) treated fiber filter bag is first submerged 10 minutes with acetone, is taken out again with 10 points new of acetone submergence
Clock；
(10) by step (9), treated weighs after fiber filter bag dries to constant weight, and is denoted as m2g；The neutral detergent calculated in feed is fine
Dimension hplc: neutral detergent fiber (%)=100% × (m2-m0)/(m1-m0)；
(11) by step (10) treated fiber filter bag with the Thermostable α-Amylase acidic cleaning liquid of 10000u/L slightly boiled
It is washed 70 minutes under state；
(12) it after rinsing step (11) treated fiber filter bag 30 minutes with cold water, is rinsed 10 minutes with the boiling water of flowing；
(13) by step (12) treated fiber filter bag submerges in acetone after ten minutes, take out and submerge 10 with new acetone again
Minute；
(14) by step (13), treated weighs after fiber filter bag dries to constant weight, and gross weight is denoted as m3g；Calculate the acidity in feed
Wash fiber content: acid detergent fiber (%)=100% × (m3-m0)/(m1-m0)。
2. measuring method according to claim 1, it is characterised in that: in step (1), according to the spy of the Feed Sample of acquisition
Property, which is processed, to be specifically included, and the feed high for moisture content is first dried, its moisture is measured；For longer or
Thicker roughage is first cut short or is cut carefully, then carries out crushing and crossing 1mm sieve.
3. measuring method according to claim 1, it is characterised in that: in step (3), 105 DEG C of drying temperature, drying time
2 hours.
4. measuring method according to claim 1, it is characterised in that: in step (5), the aperture of nylon wire is 1mm.
5. measuring method according to claim 1, it is characterised in that: in step (5), the temperature of pectinase solution is 50
DEG C, the concussion processing time is 8 hours.
6. measuring method according to claim 1, it is characterised in that: in step (6), the temperature of alpha-amylase solution is 95
DEG C, the concussion processing time 16 hours.
7. measuring method according to claim 1, it is characterised in that: in step (7), washing is washed under slight boiling condition
70 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910010577.3A CN109490136A (en) | 2019-01-07 | 2019-01-07 | A kind of acid and neutral detergent fiber content method in quick measurement feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910010577.3A CN109490136A (en) | 2019-01-07 | 2019-01-07 | A kind of acid and neutral detergent fiber content method in quick measurement feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109490136A true CN109490136A (en) | 2019-03-19 |
Family
ID=65714083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910010577.3A Pending CN109490136A (en) | 2019-01-07 | 2019-01-07 | A kind of acid and neutral detergent fiber content method in quick measurement feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109490136A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5370007A (en) * | 1993-06-11 | 1994-12-06 | Komarek; Andrew R. | Fiber analysis system |
| CN102175820A (en) * | 2011-01-26 | 2011-09-07 | 中国农业科学院草原研究所 | Application of acrylics and chinlon blending filter bag in measuring forage grass fiber content |
| US20120156799A1 (en) * | 2009-08-25 | 2012-06-21 | Ankom Technology Corporation | automated method and system for the analysis of total dietary fiber |
| CN102539274A (en) * | 2012-01-11 | 2012-07-04 | 山东农业大学 | Fiber screen bag and method for rapidly determining coarse fiber |
| CN106124358A (en) * | 2016-07-07 | 2016-11-16 | 上海交通大学 | A kind of measure the method washing fiber content in sweet sorghum stalk residue |
-
2019
- 2019-01-07 CN CN201910010577.3A patent/CN109490136A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5370007A (en) * | 1993-06-11 | 1994-12-06 | Komarek; Andrew R. | Fiber analysis system |
| US20120156799A1 (en) * | 2009-08-25 | 2012-06-21 | Ankom Technology Corporation | automated method and system for the analysis of total dietary fiber |
| CN102175820A (en) * | 2011-01-26 | 2011-09-07 | 中国农业科学院草原研究所 | Application of acrylics and chinlon blending filter bag in measuring forage grass fiber content |
| CN102539274A (en) * | 2012-01-11 | 2012-07-04 | 山东农业大学 | Fiber screen bag and method for rapidly determining coarse fiber |
| CN106124358A (en) * | 2016-07-07 | 2016-11-16 | 上海交通大学 | A kind of measure the method washing fiber content in sweet sorghum stalk residue |
Non-Patent Citations (4)
| Title |
|---|
| P. J. VAN SOESTET AL.: "《Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition》", 《J DAIRY SCI》 * |
| QING-CHANG REN ET AL.: "《Effects of tributyrin supplementation on ruminal microbial protein yield, fermentation characteristics and nutrients degradability in adult Small Tail ewes》", 《ANIM SCI J》 * |
| 苏玲玲 等.: "《纤维袋测定饲料原料中NDF和ADF 含量方法的研究》", 《饲料工业》 * |
| 袁胜 等.: "《高温α-淀粉酶对玉米、豆粕、玉米秸秆及黄豆秸秆中性洗涤纤维的影响》", 《安徽科技学院学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Van Soest et al. | Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition | |
| Van Soest et al. | Symposium: carbohydrate methodology, metabolism, and nutritional implications in dairy cattle | |
| Hall et al. | A method for partitioning neutral detergent‐soluble carbohydrates | |
| Schroeder | Forage nutrition for ruminants | |
| CN103868778B (en) | The measuring method of herbaceous plant content of lignin | |
| CN103053808B (en) | Silage additive and preparation method and application thereof | |
| Schaller | Fiber content and structure in foods | |
| Daniel et al. | Fibre digestibility and its relationships with chemical and morphological traits in thirty‐two sugarcane varieties | |
| CN109813709A (en) | A kind of acid value of dilated food and the measuring method of peroxide value | |
| Robertson | The detergent system of fiber analysis | |
| Blümmel et al. | Improving the production and utilization of sorghum and pearl millet as livestock feed: methodological problems and possible solutions | |
| CN109490136A (en) | A kind of acid and neutral detergent fiber content method in quick measurement feed | |
| Foster et al. | In sacco degradation kinetics of fresh and field-cured peanut (Arachis hypogaea L.) forage harvested at different maturities | |
| Buckner et al. | Evaluation of modifications to the neutral-detergent-fiber analysis procedure for corn and distillers grains plus solubles | |
| CN102735567B (en) | Method for determining neutral detergent fiber content in pasture | |
| Chaves et al. | A simplified method for lignin measurement in a range of forage species | |
| Mertens | Measurements of forage quality. | |
| CN102175820A (en) | Application of acrylics and chinlon blending filter bag in measuring forage grass fiber content | |
| Mlambo et al. | In vitro biological activity of tannins from Acacia and other tree fruits: Correlations with colorimetric and gravimetric phenolic assays | |
| CN107739748A (en) | A kind of assay method of Pepsin digestibility and its application | |
| Jovanović et al. | Acid-base, electrolyte and oxidative status in dairy cows at different stages of the production cycle | |
| Sharafi et al. | Determination of nutritive value of Sallow and Service leaves using nylon bags and gas production techniques | |
| CN108835428A (en) | A kind of growing and fattening pigs full price fermentative feedstuff of microbe preparation method | |
| CN101833006A (en) | Method for preparing test paper for rapidly determining dairy cows subclinical ketosis | |
| CN101845479A (en) | Method for identifying quality of ova of Acanthogobius ommaturus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190319 |