CN102735567B - Method for determining neutral detergent fiber content in pasture - Google Patents
Method for determining neutral detergent fiber content in pasture Download PDFInfo
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- CN102735567B CN102735567B CN201110092444.9A CN201110092444A CN102735567B CN 102735567 B CN102735567 B CN 102735567B CN 201110092444 A CN201110092444 A CN 201110092444A CN 102735567 B CN102735567 B CN 102735567B
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- 230000007935 neutral effect Effects 0.000 title claims abstract description 67
- 239000000835 fiber Substances 0.000 title claims abstract description 56
- 239000003599 detergent Substances 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000004321 preservation Methods 0.000 claims abstract description 25
- 239000012065 filter cake Substances 0.000 claims abstract description 22
- 238000001914 filtration Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000000243 solution Substances 0.000 claims description 20
- 238000003556 assay Methods 0.000 claims description 17
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 14
- 239000004459 forage Substances 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 8
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 7
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 5
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 5
- 235000019800 disodium phosphate Nutrition 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 2
- 235000011007 phosphoric acid Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 238000005259 measurement Methods 0.000 abstract description 13
- 238000009835 boiling Methods 0.000 abstract description 10
- 238000010438 heat treatment Methods 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 2
- 238000005406 washing Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract 3
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 abstract 1
- 238000007872 degassing Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 238000001816 cooling Methods 0.000 description 13
- 241000209094 Oryza Species 0.000 description 8
- 235000007164 Oryza sativa Nutrition 0.000 description 8
- 240000008042 Zea mays Species 0.000 description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 8
- 235000005822 corn Nutrition 0.000 description 8
- 235000009566 rice Nutrition 0.000 description 8
- 239000004460 silage Substances 0.000 description 8
- 241000743756 Bromus inermis Species 0.000 description 7
- 241000219823 Medicago Species 0.000 description 7
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 7
- 241001494479 Pecora Species 0.000 description 7
- 238000007605 air drying Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 238000009423 ventilation Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000000967 suction filtration Methods 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 125000004855 decalinyl group Chemical group C1(CCCC2CCCCC12)* 0.000 description 2
- 238000011005 laboratory method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 229940101006 anhydrous sodium sulfite Drugs 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The present invention provides a method for determining acid detergent fiber content in pasture. The method includes the following steps: (1) mixing and placing a neutral washing liquid and the pasture with a weight of A in a preservation bottle, and placing the preservation of the bottle in a pressure cooker, and heating to boiling for degassing; (2) sealing the preservation bottle and pressurizing the bottle in the pressure cooker to obtain a mixed liquid; (3) filtering the mixed liquid obtained in the step (2) with a filter with a weight of B to obtain a filter cake namely the neutral detergent fiber; and (4) drying the filter and the filter cake to a constant weight of C and then calculating according to a formula (I) to obtain the neutral detergent fiber (NDF) content in the pasture (%). Through depth analysis of NDF measurement principle, the present invention develops a simple method for determining NDF content in pasture.
Description
Technical field
The present invention relates to a kind of assay method of herbage neutral detergent fiber content, belong to herbage detection technique field.
Background technology
Neutral detergent fiber (NDF) is one of test index detecting cell wall constituent in plant feed or fibre composition, is substantially equivalent to hemicellulose in feed, cellulose and lignin sum.This of daily ration index to maintain ruminant digestive function and healthy, ensure that animal product production level is significant.
Fan Shi (Van Soest) washing the fibre method is to measure the classical way of NDF, and the method adopts neutral cleansing solution that the cellular content in plant feed is dissolved, and insoluble residue is NDF.The mensuration in laboratory is mainly divided into two kinds of modes at present: traditional suction filtration mode and the filter bag technology of dependence on import analyser.
Suction method is the classical way of measuring, and utilizes electric furnace in beaker, to complete the process of boiling that disappears, and the potpourri after then boiling disappearing obtains undissolved residue by the step such as suction filtration, cleaning.Adopt the method experimental procedure loaded down with trivial details, use chemical reagent to process more, and the sample size of once measuring is few, need to use vacuum filtration device, to extracting time requirement harshness.Filter bag technology is the method with Canada's cooperative development by the U.S., the analysis filter bag that employing special material is made is as the carrier of measuring, utilize fiber instrument to complete mensuration process, in mensuration process, fiber instrument can complete mensuration program automatically, have that automaticity is high, sample determination amount is large, accuracy high, but the cost of equipment and filter bag is very expensive.In addition, said method is laboratory method, must rely on laboratory condition to carry out testing, comparatively strict to requirement for experiment condition, should not carry out mensuration in production scene, need in production practices instant while understanding feed quality, often be difficult to guarantee ageing.
Summary of the invention
The object of this invention is to provide a kind of assay method of herbage neutral detergent fiber content.
The assay method of herbage neutral detergent fiber content provided by the invention, comprises the steps:
(1) the described herbage that is A by neutral cleansing solution and weight mixes to be placed on and preserves in bottle, by described preservations bottle be placed in pressure cooker be heated to seethe with excitement carry out degassed;
(2) described preservation bottle is sealed rear with pressurizeing in pressure cooker, obtain mixed liquor;
(3) filtrator that is B by weight carries out the mixed liquor that filtration step (2) obtains, and the filter cake obtaining is described neutral detergent fiber;
(4) by described filtrator and filtration cakes torrefaction to constant weight C, then calculate and obtain the NDF of neutral detergent fiber content described in described herbage (%) according to formula (I),
NDF(％)＝(C-B)/A×100％
(I)。
In above-mentioned assay method, described in step (1), herbage can be powder, and the particle diameter of described powder can be 18 order-40 orders, specifically can be 18 orders, 25 orders, 30 orders, 35 orders or 40 orders, is easy to the dissolving of described herbage.
In above-mentioned assay method, described herbage can be leguminous forage, graminous pasture or forage crop, as alfalfa, sheep's hay, Bai Sanye, awnless brome, whole corn silage feed or forage rice.
In above-mentioned assay method, also comprise before degassed described in step (1) to the described step that adds AMS in bottle of preserving, be applicable to the mensuration of the herbage neutral detergent fiber content that content of starch is high; The ratio of quality and the number of copies of described AMS and described herbage can be 1: (10-20), and as 1: 10 or 1: 20.
In above-mentioned assay method, described neutral cleansing solution can be the mixed aqueous solution of lauryl sodium sulfate, sodium hydrogen phosphate and phosphoric acid, and described mixed aqueous solution is prepared in accordance with the following methods: by 30g lauryl sodium sulfate, 11.48g sodium hydrogen phosphate (Na
2hPO412H
2o) soluble in water and be adjusted to 1L, be that 8.5% phosphoric acid solution regulates pH value to 7.0 and get final product with quality percentage composition.
In above-mentioned assay method, described in step (1), the degassed time can be 10min-12min, specifically can be 10min or 12min.
In above-mentioned assay method, described in step (2), the pressure of pressurization can be 150kpa-170kpa, specifically can be 150kpa, 160kpa or 170kpa, and the time of described pressurization can be 40min.
In above-mentioned assay method, described filtrator is filter paper, and the aperture of described filter paper can be 80 μ m-120 μ m, specifically can be 80 μ m, 100 μ m, 110 μ m or 120 μ m.
In above-mentioned assay method, before filtering described in step (3), also comprise the step that with micro-wave oven, described filter paper is dried to constant weight.
In above-mentioned assay method, described in step (4), be dried in micro-wave oven and carry out.
The present invention has the following advantages:
(1) simplified drug variety and the layoutprocedure of neutral cleansing solution, laboratory method needs to use 8 kinds of chemical reagent in mensuration process, and method provided by the invention has adopted 3 kinds of main agents can complete mensuration;
(2) equipment adopting in mensuration process is all conventional kitchen equipment in life, and without complete laboratory condition, the condition of mensuration is content with very little, and has improved measure ageing;
(3) in mensuration process, gordian technique main points are easy to grasp, and all processes is easy to grasp, and without technical professional, mensuration personnel can grasp through simple training.
Embodiment
The experimental technique using in following embodiment if no special instructions, is conventional method.
Material, reagent etc. used in following embodiment, if no special instructions, all can obtain from commercial channels.
Neutral cleansing solution in the following embodiment of the present invention is prepared in accordance with the following methods: by 30g lauryl sodium sulfate, 11.48g sodium hydrogen phosphate (Na
2hPO412H
2o) soluble in water and be adjusted to 1L, be that 8.5% phosphoric acid solution regulates pH value to 7.0 and get final product with quality percentage composition.
The phosphoric acid solution that in above-mentioned compound method, quality percentage composition is 8.5% is demarcated in accordance with the following methods: by soluble in water 10ml phosphoric acid (quality percentage composition is 85%), and water is settled to 100ml and get final product.
In the following embodiment of the present invention, the concrete steps of classical way used are as follows:
Reagent and preparation:
(1) 30gL-1 lauryl sodium sulfate (neutral detergent) solution.Take that 18.61g ethylenediamine tetraacetic acid is received (EDTA) and 6.81g sodium tetraborate (Na
2b
4o
710H
2o) with putting into 1000mL beaker, add after a small amount of water heating for dissolving, then add 30g lauryl sodium sulfate.Take 4.65g ADSP, be placed in another beaker, add a small amount of water, heating for dissolving hypsokinesis, in first beaker, is diluted to 100mL slightly.This pH value of solution is 6.9～7.0.
(2) other reagent: acetone, anhydrous sodium sulfate, decahydronaphthalenes.
Determination step:
1. accurately take 40 mesh sieve Feed Sample 0.5～1.0g (m), put without in mouth beaker in tall form, added neutral detergent solution 100mL and 2mL decahydronaphthalenes and 0.5g anhydrous sodium sulfite.
2. load onto condensing unit, be placed on electric furnace immediately and boil (5～10min), and micro-1h that boils.
3. boil completely, cooling 10min, is installed on the glass pot of known quality (ml) on bottle,suction, residue is all moved into, and suction filtration, and rinse with boiling water, then suction filtration.Use again 20mL acetone rinsing, suction filtration.
4. take off crucible, 105 DEG C of oven dry, (m weighs
2).
Result is calculated
In formula: m
2for crucible+NDF quality, g; m
1for crucible quality, g; M is sample mass, g.
The mensuration of embodiment 1, alfalfa neutral detergent fiber content
(1) alfalfa is cut short to 1～2cm, be placed in dry ventilated environment natural air drying.It is 18 orders that air-dry sample is milled to particle diameter with food masher.
(2) above-mentioned alfalfa 1.03g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml); Screw on preservation bottle cap, appropriateness screws to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to bottleneck (under bottleneck protruding place), be placed on fire.After boiling, the weak fluidized state of furnishing keeps 10 minutes, carries out degassed.Not lid of this pressure process pot.
(3) preservation bottle cap is tightened, water is added to bottleneck, the lid that covers pressure cooker is placed in fire above, keeps 40 minutes pressurized states (150kpa), obtains the mixed liquor that contains neutral detergent fiber.Then, naturally cooling 10～15 minutes, open pressure cooker, take out bottle, then by naturally cooling bottle 10 minutes.
(4) use and be dried to the coffee filter paper of constant weight through micro-wave oven (power is 200W) (weight is 1.19g, aperture is that the mixed liquor that 100 μ m) obtain step (3) filters, and the filter cake obtaining is neutral detergent fiber; Wash filter cake with water twice (40ml × 2).
(5) filter cake obtained above (comprising coffee filter paper) is dried with micro-wave oven to (power is 500W, and the time is 3 minutes; Power is 200W, and the time is 7 minutes), being dried to constant weight quality is 1.65g; The content NDF (%) that calculates and obtain alfalfa neutral detergent fiber according to formula (I) is 44.66%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates alfalfa neutral detergent fiber according to formula (I) is respectively 47.67% and 47.48%.
The content NDF (%) that measures alfalfa neutral detergent fiber with classical way, measurement result is in triplicate respectively 45.06%, 48.45% and 46.32%; Known, method measurement result provided by the invention is more accurate.
The mensuration of embodiment 2, sheep's hay neutral detergent fiber content
(1) sheep's hay is cut short to 1～2cm, be placed in dry ventilated environment natural air drying.It is 40 orders that air-dry sample is milled to particle diameter with food masher.
(2) above-mentioned sheep's hay 0.97g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml); Screw on preservation bottle cap, appropriateness screws to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to bottleneck (under bottleneck protruding place), be placed on fire.After boiling, the weak fluidized state of furnishing keeps 10 minutes, carries out degassed.Not lid of this pressure process pot.
(3) preservation bottle cap is tightened, water is added to bottleneck, the lid that covers pressure cooker is placed in fire above, keeps 40 minutes pressurized states (170kpa), obtains the mixed liquor that contains neutral detergent fiber.Then, naturally cooling 10～15 minutes, open pressure cooker, take out bottle, then by naturally cooling bottle 15 minutes.
(4) use and be dried to the coffee filter paper of constant weight through micro-wave oven (power is 200W) (weight is 1.20g, aperture is that the mixed liquor that 120 μ m) obtain step (3) filters, and the filter cake obtaining is neutral detergent fiber; Wash filter cake with water twice (40ml × 2).
(5) filter cake obtained above (comprising coffee filter paper) is dried with micro-wave oven to (power is 500W, and the time is 3 minutes; Power is 200W, and the time is 7 minutes), being dried to constant weight quality is 1.84g; The content NDF (%) that calculates and obtain sheep's hay neutral detergent fiber according to formula (I) is 65.98%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates sheep's hay neutral detergent fiber according to formula (I) is respectively 59.78% and 56.33%.
The content NDF (%) that measures sheep's hay neutral detergent fiber with classical way, measurement result is in triplicate respectively 65.10%, 60.87% and 56.17%; Known, method measurement result provided by the invention is more accurate.
The mensuration of embodiment 3, Bai Sanye neutral detergent fiber content
(1) Bai Sanye is cut short to 1～2cm, be placed in dry ventilated environment natural air drying.It is 30 orders that air-dry sample is milled to particle diameter with food masher.
(2) above-mentioned Bai Sanye 1.08g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml); Screw on preservation bottle cap, appropriateness screws to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to bottleneck (under bottleneck protruding place), be placed on fire.After boiling, the weak fluidized state of furnishing keeps 10 minutes, carries out degassed.Not lid of this pressure process pot.
(3) preservation bottle cap is tightened, water is added to bottleneck, the lid that covers pressure cooker is placed in fire above, keeps 40 minutes pressurized states (160kpa), obtains the mixed liquor that contains neutral detergent fiber.Then, naturally cooling 10～15 minutes, open pressure cooker, take out bottle, then by naturally cooling bottle 10 minutes.
(4) use and be dried to the coffee filter paper of constant weight through micro-wave oven (power is 200W) (weight is 1.21g, aperture is that the mixed liquor that 80 μ m) obtain step (3) filters, and the filter cake obtaining is neutral detergent fiber; Wash filter cake with water twice (40ml × 2).
(5) filter cake obtained above (comprising coffee filter paper) is dried with micro-wave oven to (power is 500W, and the time is 3 minutes; Power is 200W, and the time is 7 minutes), being dried to constant weight quality is 1.54g; The content NDF (%) that calculates and obtain Bai Sanye neutral detergent fiber according to formula (I) is 30.56%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates Bai Sanye neutral detergent fiber according to formula (I) is respectively 33.60% and 22.63%.
The content NDF (%) that measures Bai Sanye neutral detergent fiber with classical way, measurement result is in triplicate respectively 31.23%, 32.34% and 23.27%; Known, method measurement result provided by the invention is more accurate.
The mensuration of embodiment 4, awnless brome neutral detergent fiber content
(1) awnless brome is cut short to 1～2cm, be placed in dry ventilated environment natural air drying.It is 25 orders that air-dry sample is milled to particle diameter with food masher.
(2) above-mentioned awnless brome 1.01g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml); Screw on preservation bottle cap, appropriateness screws to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to bottleneck (under bottleneck protruding place), be placed on fire.After boiling, the weak fluidized state of furnishing keeps 12 minutes, carries out degassed.Not lid of this pressure process pot.
(3) preservation bottle cap is tightened, water is added to bottleneck, the lid that covers pressure cooker is placed in fire above, keeps 40 minutes pressurized states (150kpa), obtains the mixed liquor that contains neutral detergent fiber.Then, naturally cooling 10～15 minutes, open pressure cooker, take out bottle, then by naturally cooling bottle 15 minutes.
(4) use and be dried to the coffee filter paper of constant weight through micro-wave oven (power is 200W) (weight is 1.19g, aperture is that the mixed liquor that 100 μ m) obtain step (3) filters, and the filter cake obtaining is neutral detergent fiber; Wash filter cake with water twice (40ml × 2).
(5) filter cake obtained above (comprising coffee filter paper) is dried with micro-wave oven to (power is 500W, and the time is 3 minutes; Power is 200W, and the time is 7 minutes), being dried to constant weight quality is 1.79g; The content NDF (%) that calculates and obtain awnless brome neutral detergent fiber according to formula (I) is 59.41%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates awnless brome neutral detergent fiber according to formula (I) is respectively 55.40% and 59.73%.
The content NDF (%) that measures awnless brome neutral detergent fiber with classical way, measurement result is in triplicate respectively 59.40%, 56.67% and 58.97%; Known, method measurement result provided by the invention is more accurate.
The mensuration of embodiment 5, whole corn silage feed neutral detergent fiber content
(1) whole corn silage feed is placed in to dry ventilated environment natural air drying.It is 35 orders that air-dry sample is milled to particle diameter with food masher.
(2) above-mentioned whole corn silage feed 1.07g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml), add the high temperature resistant AMS of 50 μ L (wherein, AMS and whole corn silage quality of the fodder portion rate are 1: 10); Screw on preservation bottle cap, appropriateness screws to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to bottleneck (under bottleneck protruding place), be placed on fire.After boiling, the weak fluidized state of furnishing keeps 12 minutes, carries out degassed.Not lid of this pressure process pot.
(3) preservation bottle cap is tightened, water is added to bottleneck, the lid that covers pressure cooker is placed in fire above, keeps 40 minutes pressurized states (150kpa), obtains the mixed liquor that contains neutral detergent fiber.Then, naturally cooling 10 minutes, open pressure cooker, take out bottle, then by naturally cooling bottle 15 minutes.
(4) use and be dried to the coffee filter paper of constant weight through micro-wave oven (power is 200W) (weight is 1.23g, aperture is that the mixed liquor that 110 μ m) obtain step (3) filters, and the filter cake obtaining is neutral detergent fiber; Wash filter cake with water twice (40ml × 2).
(5) filter cake obtained above (comprising coffee filter paper) is dried with micro-wave oven to (power is 500W, and the time is 3 minutes; Power is 200W, and the time is 7 minutes), being dried to constant weight quality is 1.82g; The content NDF (%) that calculates and obtain whole corn silage feed neutral detergent fiber according to formula (I) is 55.14%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates whole corn silage feed neutral detergent fiber according to formula (I) is respectively 54.88% and 47.79%.
The content NDF (%) that measures whole corn silage feed neutral detergent fiber with classical way, measurement result is in triplicate respectively 55.84%, 54.27% and 47.84%; Known, method measurement result provided by the invention is more accurate.
The mensuration of embodiment 6, forage rice neutral detergent fiber content
(1) forage rice is placed in to dry ventilated environment natural air drying.It is 30 orders that air-dry sample is milled to particle diameter with food masher.
(2) above-mentioned forage rice 1.10g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml), add the high temperature resistant AMS of 50 μ L (wherein, the ratio of quality and the number of copies of AMS and forage rice is 1: 20); Screw on preservation bottle cap, appropriateness screws to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to bottleneck (under bottleneck protruding place), be placed on fire.After boiling, the weak fluidized state of furnishing keeps 10 minutes, carries out degassed.Not lid of this pressure process pot.
(3) preservation bottle cap is tightened, water is added to bottleneck, the lid that covers pressure cooker is placed in fire above, keeps 40 minutes pressurized states (160kpa), obtains the mixed liquor that contains neutral detergent fiber.Then, naturally cooling 10 minutes, open pressure cooker, take out bottle, then by naturally cooling bottle 15 minutes.
(4) use and be dried to the coffee filter paper of constant weight through micro-wave oven (power is 200W) (weight is 1.21g, aperture is that the mixed liquor that 120 μ m) obtain step (3) filters, and the filter cake obtaining is neutral detergent fiber; Wash filter cake with water twice (40ml × 2).
(5) filter cake obtained above (comprising coffee filter paper) is dried with micro-wave oven to (power is 500W, and the time is 3 minutes; Power is 200W, and the time is 7 minutes), being dried to constant weight quality is 1.77g; The content NDF (%) that calculates and obtain forage rice neutral detergent fiber according to formula (I) is 50.91%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates forage rice neutral detergent fiber according to formula (I) is respectively 45.13% and 56.29%.
The content NDF (%) that measures forage rice neutral detergent fiber with classical way, measurement result is in triplicate respectively 51.82%, 46.28% and 56.17%; Known, method measurement result provided by the invention is more accurate.
Claims (4)
1. the assay method of herbage neutral detergent fiber content, comprises the steps:
(1) the described herbage that is A by neutral cleansing solution and weight mixes to be placed on and preserves in bottle, by described preservations bottle be placed in pressure cooker be heated to seethe with excitement carry out degassed; Described also comprising before degassed to the described step that adds AMS in bottle of preserving, be applicable to the mensuration of the herbage neutral detergent fiber content that content of starch is high; The ratio of quality and the number of copies of described AMS and described herbage can be 1:(10-20); The described degassed time is 10 minutes-12 minutes; Described herbage is powder, and the particle diameter of described powder is 18 order-40 orders; Described neutral cleansing solution can be the mixed aqueous solution of lauryl sodium sulfate, sodium hydrogen phosphate and phosphoric acid, and described mixed aqueous solution is prepared in accordance with the following methods: by 30g lauryl sodium sulfate, 11.48g sodium hydrogen phosphate (Na
2hPO412H
2o) soluble in water and be adjusted to 1L, be that 8.5% phosphoric acid solution regulates pH value to 7.0 and get final product with quality percentage composition;
(2) described preservation bottle is sealed rear with pressurizeing in pressure cooker, obtain mixed liquor; The pressure of described pressurization is 150kpa-170kpa, and the time of described pressurization is 40 minutes;
(3) filtrator that is B by weight carries out the described mixed liquor that filtration step (2) obtains, and the filter cake obtaining is described neutral detergent fiber; Before described filtration, also comprise the step that with micro-wave oven, described filter paper is dried to constant weight;
(4) by described filtrator and filtration cakes torrefaction to constant weight C, then calculate and obtain the NDF(% of neutral detergent fiber content described in described herbage according to formula (I)),
NDF（%）=（C-B）/A×100%
（Ⅰ）。
2. assay method according to claim 1, is characterized in that: described herbage is leguminous forage, graminous pasture or forage crop.
3. assay method according to claim 2, is characterized in that: described filtrator is filter paper, and the aperture of described filter paper is 80 μ m-120 μ m.
4. assay method according to claim 3, is characterized in that: described in step (4), be dried in micro-wave oven and carry out.
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| CN108020483A (en) * | 2018-01-25 | 2018-05-11 | 杭州神农牧业有限公司 | Neutral detergent fiber measurement device |
| CN113627660B (en) * | 2021-07-30 | 2023-11-10 | 深圳市中金岭南有色金属股份有限公司凡口铅锌矿 | Method and device for predicting productivity of ceramic filter |
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| 中华人民共和国国家质量监督检验检疫总局中国国家标准化管理委员会.饲料中中性洗涤纤维(NDF)的测定.《中华人民共和国国家标准GB/T20806一200》.2006,第1-3页. * |
| 玉米秸秆NDF与ADF含量变化规律的研究;朱顺国 等;《实验研究》;20011231(第1期);第24-26页 * |
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