CN102735567A - Method for determining neutral detergent fiber content in pasture - Google Patents
Method for determining neutral detergent fiber content in pasture Download PDFInfo
- Publication number
- CN102735567A CN102735567A CN2011100924449A CN201110092444A CN102735567A CN 102735567 A CN102735567 A CN 102735567A CN 2011100924449 A CN2011100924449 A CN 2011100924449A CN 201110092444 A CN201110092444 A CN 201110092444A CN 102735567 A CN102735567 A CN 102735567A
- Authority
- CN
- China
- Prior art keywords
- bottle
- detergent fiber
- assay method
- neutral detergent
- ndf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000007935 neutral effect Effects 0.000 title claims abstract description 66
- 239000000835 fiber Substances 0.000 title claims abstract description 55
- 239000003599 detergent Substances 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 40
- 238000004321 preservation Methods 0.000 claims abstract description 19
- 239000012065 filter cake Substances 0.000 claims abstract description 16
- 238000001035 drying Methods 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000007872 degassing Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 19
- 238000003556 assay Methods 0.000 claims description 18
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 12
- 239000004459 forage Substances 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 8
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 6
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 6
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 4
- 235000019800 disodium phosphate Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 235000011007 phosphoric acid Nutrition 0.000 claims description 2
- 238000009835 boiling Methods 0.000 abstract description 10
- 238000005406 washing Methods 0.000 abstract description 8
- 238000010438 heat treatment Methods 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract 3
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 25
- 238000001816 cooling Methods 0.000 description 13
- 241000209094 Oryza Species 0.000 description 8
- 235000007164 Oryza sativa Nutrition 0.000 description 8
- 240000008042 Zea mays Species 0.000 description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 8
- 235000005822 corn Nutrition 0.000 description 8
- 235000009566 rice Nutrition 0.000 description 8
- 239000004460 silage Substances 0.000 description 8
- 241000743756 Bromus inermis Species 0.000 description 7
- 241000219823 Medicago Species 0.000 description 7
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 7
- 241001494479 Pecora Species 0.000 description 7
- 238000007605 air drying Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 238000002386 leaching Methods 0.000 description 6
- 238000009423 ventilation Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000000967 suction filtration Methods 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 125000004855 decalinyl group Chemical group C1(CCCC2CCCCC12)* 0.000 description 2
- 238000011005 laboratory method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229940101006 anhydrous sodium sulfite Drugs 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a method for determining acid detergent fiber content in pasture. The method includes the following steps: (1) mixing and placing a neutral washing liquid and the pasture with a weight of A in a preservation bottle, and placing the preservation of the bottle in a pressure cooker, and heating to boiling for degassing; (2) sealing the preservation bottle and pressurizing the bottle in the pressure cooker to obtain a mixed liquid; (3) filtering the mixed liquid obtained in the step (2) with a filter with a weight of B to obtain a filter cake namely the neutral detergent fiber; and (4) drying the filter and the filter cake to a constant weight of C and then calculating according to a formula (I) to obtain the neutral detergent fiber (NDF) content in the pasture (%). Through depth analysis of NDF measurement principle, the present invention develops a simple method for determining NDF content in pasture.
Description
Technical field
The present invention relates to a kind of herbage neutral detergent fiber Determination on content method, belong to herbage detection technique field.
Background technology
Neutral detergent fiber (NDF) is one of test index that detects cell wall constituent in the plant feed or fibre composition, is equivalent to hemicellulose in the feed, cellulose and lignin sum basically.This of daily ration index to the digestive function of keeping ruminant with healthy, ensure that the animal product production level is significant.
Fan Shi (Van Soest) washing the fibre method is to measure the classical way of NDF, and this method adopts neutral cleansing solution with the dissolving of the cellular content in the plant feed, and insoluble residue is NDF.Breadboard mensuration mainly is divided into dual mode at present: traditional suction filtration mode and the filter bag of dependence on import analyser technology.
Suction method is the classical way of measuring, and utilizes electric furnace in beaker, to accomplish the process of boiling that disappears, and the potpourri that will disappear after boiling then obtains undissolved residue through steps such as suction filtration, cleanings.Adopt this method experimental procedure loaded down with trivial details, it is more to use chemical reagent to handle, and the sample size of once measuring is few, needs to use the vacuum filtration device, harsh to extracting time requirement.The filter bag technology is by the method for the U.S. with Canada's cooperative development; The analysis filter bag that the employing special material is processed is as the carrier of measuring; Utilize the fiber appearance to accomplish the mensuration process; The fiber appearance can be accomplished the mensuration program automatically in the mensuration process, have characteristics such as automaticity height, sample determination amount are big, accuracy height, but the cost of equipment and filter bag is very expensive.In addition, said method is laboratory method, must rely on laboratory condition to carry out testing; Comparatively strict to requirement for experiment condition; Should not carry out mensuration in the production scene, need in production practices instant when understanding feed quality, often be difficult to guarantee ageing.
Summary of the invention
The purpose of this invention is to provide a kind of herbage neutral detergent fiber Determination on content method.
Herbage neutral detergent fiber Determination on content method provided by the invention comprises the steps:
(1) is that the said herbage of A mixes to be placed on and preserves in the bottle with neutral cleansing solution and weight, said preservations bottle placed pressure cooker to be heated to seethe with excitement outgas;
(2) said preservation bottle is sealed the back with pressurizeing in the pressure cooker, obtain mixed liquor;
(3) use weight to carry out the mixed liquor that filtration step (2) obtains as the filtrator of B, the filter cake that obtains is said neutral detergent fiber;
(4) with said filtrator and filtration cakes torrefaction to constant weight C, calculate according to formula (I) then and promptly get neutral detergent fiber content NDF (%) described in the then said herbage,
NDF(％)＝(C-B)/A×100％
(I)。
In the above-mentioned assay method, herbage can be powder described in the step (1), and the particle diameter of said powder can be 18 orders-40 order, specifically can be 18 orders, 25 orders, 30 orders, 35 orders or 40 orders, is easy to the dissolving of said herbage.
In the above-mentioned assay method, said herbage can be leguminous forage, graminous pasture or forage crop, like alfalfa, sheep's hay, Bai Sanye, awnless brome, whole corn silage feed or forage rice.
In the above-mentioned assay method, also comprise before the degassing described in the step (1), be applicable to the herbage neutral detergent fiber Determination on content that content of starch is high to the said step that adds AMS in the bottle of preserving; The ratio of quality and the number of copies of said AMS and said herbage can be 1: (10-20), and as 1: 10 or 1: 20.
In the above-mentioned assay method, said neutral cleansing solution can be the mixed aqueous solution of lauryl sodium sulfate, sodium hydrogen phosphate and phosphoric acid, and said mixed aqueous solution is prepared according to following method: with 30g lauryl sodium sulfate, 11.48g sodium hydrogen phosphate (Na
2HPO412H
2O) soluble in water and transfer to 1L, using the quality percentage composition is that 8.5% phosphoric acid solution is regulated pH value to 7.0 and promptly got.
In the above-mentioned assay method, the time of the degassing described in the step (1) can be 10min-12min, specifically can be 10min or 12min.
In the above-mentioned assay method, the pressure of pressurization can be 150kpa-170kpa described in the step (2), specifically can be 150kpa, 160kpa or 170kpa, and the time of said pressurization can be 40min.
In the above-mentioned assay method, said filtrator is a filter paper, and the aperture of said filter paper can be 80 μ m-120 μ m, specifically can be 80 μ m, 100 μ m, 110 μ m or 120 μ m.
In the above-mentioned assay method, also comprise the step that said filter paper is dried to constant weight with micro-wave oven before filtering described in the step (3).
In the above-mentioned assay method, drying is carried out in micro-wave oven described in the step (4).
The present invention has the following advantages:
(1) simplified the drug variety and the layoutprocedure of neutral cleansing solution, laboratory method needs to use 8 kinds of chemical reagent in the mensuration process, and method provided by the invention has adopted 3 kinds of main agents can accomplish mensuration;
(2) equipment that adopts in the mensuration process all is a kitchen equipment commonly used in the life, need not complete laboratory condition, and the condition of mensuration is content with very little, and has improved measure ageing;
(3) the gordian technique main points are easy to grasp in the mensuration process, and all processes is easy to grasp, and need not the technical professional, and mensuration personnel can grasp through simple training.
Embodiment
Employed experimental technique is conventional method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Neutral cleansing solution among the following embodiment of the present invention is prepared according to following method: with 30g lauryl sodium sulfate, 11.48g sodium hydrogen phosphate (Na
2HPO412H
2O) soluble in water and transfer to 1L, using the quality percentage composition is that 8.5% phosphoric acid solution is regulated pH value to 7.0 and promptly got.
The quality percentage composition is that 8.5% phosphoric acid solution is demarcated according to following method in the above-mentioned compound method: 10ml phosphoric acid (the quality percentage composition is 85%) is soluble in water, and water is settled to 100ml and promptly gets.
The concrete steps of used classical way are as follows among the following embodiment of the present invention:
Reagent and preparation:
(1) 30gL-1 lauryl sodium sulfate (neutral detergent) solution.Take by weighing that the 18.61g ethylenediamine tetraacetic acid is received (EDTA) and 6.81g sodium tetraborate (Na
2B
4O
710H
2O) with putting into the 1000mL beaker, add the low amounts of water heating for dissolving after, add the 30g lauryl sodium sulfate again.Take by weighing the 4.65g ADSP, place another beaker, add low amounts of water, the heating for dissolving hypsokinesis is diluted to 100mL in first beaker slightly.This pH value of solution is 6.9～7.0.
(2) other reagent: acetone, anhydrous sodium sulfate, decahydronaphthalenes.
Determination step:
1. accurately take by weighing 40 mesh sieve feed sample 0.5～1.0g (m), put in the no mouth beaker in tall form, added neutral detergent solution 100mL and 2mL decahydronaphthalenes and 0.5g anhydrous sodium sulfite.
2. load onto condensing unit, place on the electric furnace immediately and boil (5～10min), and little 1h that boils.
3. boil and finish, cooling 10min is installed on the glass pot (ml) of known quality on the bottle,suction, residue is all moved into, and suction filtration, and with boiling water flushing, suction filtration again.Use the 20mL acetone rinsing again, suction filtration.
4. take off crucible, 105 ℃ of oven dry, (m weighs
2).
The result calculates
In the formula: m
2Be crucible+NDF quality, g; m
1Be crucible quality, g; M is a sample mass, g.
Embodiment 1, alfalfa neutral detergent fiber Determination on content
(1) alfalfa is cut short to 1～2cm, placed dry ventilated environment natural air drying.Using food to use masher to be milled to particle diameter air-dry sample is 18 orders.
(2) above-mentioned alfalfa 1.03g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml); Screw on the preservation bottle cap, appropriateness is screwed to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to neck (under the bottleneck protruding place), place on the fire.After the boiling, the weak fluidized state of furnishing kept 10 minutes, outgased.This pressure process pot is lid not.
(3) will preserve bottle cap and tighten, and add to neck to water, the lid that covers pressure cooker places on the fire, keeps 40 minutes pressurized states (150kpa), obtains containing the mixed liquor of neutral detergent fiber.Then, natural cooling 10～15 minutes is opened pressure cooker, takes out bottle, again with bottle natural cooling 10 minutes.
(4) use and be dried to the mixed liquor that the coffee filter paper (weight is 1.19g, and the aperture is 100 μ m) of constant weight obtains step (3) through micro-wave oven (power is 200W) and filter, the filter cake that obtains is neutral detergent fiber; Twice of water washing leaching cake (40ml * 2).
(5) (power is 500W, and the time is 3 minutes the above-mentioned filter cake that obtains (comprising coffee filter paper) to be carried out drying with micro-wave oven; Power is 200W, and the time is 7 minutes), being dried to the constant weight quality is 1.65g; Calculating the content NDF (%) that promptly gets the alfalfa neutral detergent fiber according to formula (I) is 44.66%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates the alfalfa neutral detergent fiber according to formula (I) is respectively 47.67% and 47.48%.
With the content NDF (%) of classical way mensuration alfalfa neutral detergent fiber, the mensuration result of triplicate is respectively 45.06%, 48.45% and 46.32%; Can know that it is more accurate that method provided by the invention is measured the result.
Embodiment 2, sheep's hay neutral detergent fiber Determination on content
(1) sheep's hay is cut short to 1～2cm, placed dry ventilated environment natural air drying.Using food to use masher to be milled to particle diameter air-dry sample is 40 orders.
(2) above-mentioned sheep's hay 0.97g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml); Screw on the preservation bottle cap, appropriateness is screwed to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to neck (under the bottleneck protruding place), place on the fire.After the boiling, the weak fluidized state of furnishing kept 10 minutes, outgased.This pressure process pot is lid not.
(3) will preserve bottle cap and tighten, and add to neck to water, the lid that covers pressure cooker places on the fire, keeps 40 minutes pressurized states (170kpa), obtains containing the mixed liquor of neutral detergent fiber.Then, natural cooling 10～15 minutes is opened pressure cooker, takes out bottle, again with bottle natural cooling 15 minutes.
(4) use and be dried to the mixed liquor that the coffee filter paper (weight is 1.20g, and the aperture is 120 μ m) of constant weight obtains step (3) through micro-wave oven (power is 200W) and filter, the filter cake that obtains is neutral detergent fiber; Twice of water washing leaching cake (40ml * 2).
(5) (power is 500W, and the time is 3 minutes the above-mentioned filter cake that obtains (comprising coffee filter paper) to be carried out drying with micro-wave oven; Power is 200W, and the time is 7 minutes), being dried to the constant weight quality is 1.84g; Calculating the content NDF (%) that promptly gets the sheep's hay neutral detergent fiber according to formula (I) is 65.98%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates the sheep's hay neutral detergent fiber according to formula (I) is respectively 59.78% and 56.33%.
With the content NDF (%) of classical way mensuration sheep's hay neutral detergent fiber, the mensuration result of triplicate is respectively 65.10%, 60.87% and 56.17%; Can know that it is more accurate that method provided by the invention is measured the result.
Embodiment 3, Bai Sanye neutral detergent fiber Determination on content
(1) Bai Sanye is cut short to 1～2cm, place dry ventilated environment natural air drying.Using food to use masher to be milled to particle diameter air-dry sample is 30 orders.
(2) above-mentioned Bai Sanye 1.08g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml); Screw on the preservation bottle cap, appropriateness is screwed to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to neck (under the bottleneck protruding place), place on the fire.After the boiling, the weak fluidized state of furnishing kept 10 minutes, outgased.This pressure process pot is lid not.
(3) will preserve bottle cap and tighten, and add to neck to water, the lid that covers pressure cooker places on the fire, keeps 40 minutes pressurized states (160kpa), obtains containing the mixed liquor of neutral detergent fiber.Then, natural cooling 10～15 minutes is opened pressure cooker, takes out bottle, again with bottle natural cooling 10 minutes.
(4) use and be dried to the mixed liquor that the coffee filter paper (weight is 1.21g, and the aperture is 80 μ m) of constant weight obtains step (3) through micro-wave oven (power is 200W) and filter, the filter cake that obtains is neutral detergent fiber; Twice of water washing leaching cake (40ml * 2).
(5) (power is 500W, and the time is 3 minutes the above-mentioned filter cake that obtains (comprising coffee filter paper) to be carried out drying with micro-wave oven; Power is 200W, and the time is 7 minutes), being dried to the constant weight quality is 1.54g; Calculating the content NDF (%) that promptly gets the Bai Sanye neutral detergent fiber according to formula (I) is 30.56%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates the Bai Sanye neutral detergent fiber according to formula (I) is respectively 33.60% and 22.63%.
With the content NDF (%) of classical way mensuration Bai Sanye neutral detergent fiber, the mensuration result of triplicate is respectively 31.23%, 32.34% and 23.27%; Can know that it is more accurate that method provided by the invention is measured the result.
Embodiment 4, awnless brome neutral detergent fiber Determination on content
(1) awnless brome is cut short to 1～2cm, placed dry ventilated environment natural air drying.Using food to use masher to be milled to particle diameter air-dry sample is 25 orders.
(2) above-mentioned awnless brome 1.01g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml); Screw on the preservation bottle cap, appropriateness is screwed to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to neck (under the bottleneck protruding place), place on the fire.After the boiling, the weak fluidized state of furnishing kept 12 minutes, outgased.This pressure process pot is lid not.
(3) will preserve bottle cap and tighten, and add to neck to water, the lid that covers pressure cooker places on the fire, keeps 40 minutes pressurized states (150kpa), obtains containing the mixed liquor of neutral detergent fiber.Then, natural cooling 10～15 minutes is opened pressure cooker, takes out bottle, again with bottle natural cooling 15 minutes.
(4) use and be dried to the mixed liquor that the coffee filter paper (weight is 1.19g, and the aperture is 100 μ m) of constant weight obtains step (3) through micro-wave oven (power is 200W) and filter, the filter cake that obtains is neutral detergent fiber; Twice of water washing leaching cake (40ml * 2).
(5) (power is 500W, and the time is 3 minutes the above-mentioned filter cake that obtains (comprising coffee filter paper) to be carried out drying with micro-wave oven; Power is 200W, and the time is 7 minutes), being dried to the constant weight quality is 1.79g; Calculating the content NDF (%) that promptly gets the awnless brome neutral detergent fiber according to formula (I) is 59.41%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates the awnless brome neutral detergent fiber according to formula (I) is respectively 55.40% and 59.73%.
With the content NDF (%) of classical way mensuration awnless brome neutral detergent fiber, the mensuration result of triplicate is respectively 59.40%, 56.67% and 58.97%; Can know that it is more accurate that method provided by the invention is measured the result.
Embodiment 5, whole corn silage feed neutral detergent fiber Determination on content
(1) the whole corn silage feed is placed dry ventilated environment natural air drying.Using food to use masher to be milled to particle diameter air-dry sample is 35 orders.
(2) above-mentioned whole corn silage feed 1.07g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml), add the high temperature resistant AMS of 50 μ L (wherein, AMS and whole corn silage quality of the fodder portion rate are 1: 10); Screw on the preservation bottle cap, appropriateness is screwed to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to neck (under the bottleneck protruding place), place on the fire.After the boiling, the weak fluidized state of furnishing kept 12 minutes, outgased.This pressure process pot is lid not.
(3) will preserve bottle cap and tighten, and add to neck to water, the lid that covers pressure cooker places on the fire, keeps 40 minutes pressurized states (150kpa), obtains containing the mixed liquor of neutral detergent fiber.Then, natural cooling 10 minutes is opened pressure cooker, takes out bottle, again with bottle natural cooling 15 minutes.
(4) use and be dried to the mixed liquor that the coffee filter paper (weight is 1.23g, and the aperture is 110 μ m) of constant weight obtains step (3) through micro-wave oven (power is 200W) and filter, the filter cake that obtains is neutral detergent fiber; Twice of water washing leaching cake (40ml * 2).
(5) (power is 500W, and the time is 3 minutes the above-mentioned filter cake that obtains (comprising coffee filter paper) to be carried out drying with micro-wave oven; Power is 200W, and the time is 7 minutes), being dried to the constant weight quality is 1.82g; Calculating the content NDF (%) that promptly gets whole corn silage feed neutral detergent fiber according to formula (I) is 55.14%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates whole corn silage feed neutral detergent fiber according to formula (I) is respectively 54.88% and 47.79%.
With the content NDF (%) of classical way mensuration whole corn silage feed neutral detergent fiber, the mensuration result of triplicate is respectively 55.84%, 54.27% and 47.84%; Can know that it is more accurate that method provided by the invention is measured the result.
Embodiment 6, forage rice neutral detergent fiber Determination on content
(1) forage rice is placed dry ventilated environment natural air drying.Using food to use masher to be milled to particle diameter air-dry sample is 30 orders.
(2) above-mentioned forage rice 1.10g sample and neutral cleansing solution 100ml are put into preservation bottle (a volume 150ml), add the high temperature resistant AMS of 50 μ L (wherein, the ratio of quality and the number of copies of AMS and forage rice is 1: 20); Screw on the preservation bottle cap, appropriateness is screwed to keep inside and outside ventilation.Then, bottle is put into pressure cooker, in pot, add water to neck (under the bottleneck protruding place), place on the fire.After the boiling, the weak fluidized state of furnishing kept 10 minutes, outgased.This pressure process pot is lid not.
(3) will preserve bottle cap and tighten, and add to neck to water, the lid that covers pressure cooker places on the fire, keeps 40 minutes pressurized states (160kpa), obtains containing the mixed liquor of neutral detergent fiber.Then, natural cooling 10 minutes is opened pressure cooker, takes out bottle, again with bottle natural cooling 15 minutes.
(4) use and be dried to the mixed liquor that the coffee filter paper (weight is 1.21g, and the aperture is 120 μ m) of constant weight obtains step (3) through micro-wave oven (power is 200W) and filter, the filter cake that obtains is neutral detergent fiber; Twice of water washing leaching cake (40ml * 2).
(5) (power is 500W, and the time is 3 minutes the above-mentioned filter cake that obtains (comprising coffee filter paper) to be carried out drying with micro-wave oven; Power is 200W, and the time is 7 minutes), being dried to the constant weight quality is 1.77g; Calculating the content NDF (%) that promptly gets the forage rice neutral detergent fiber according to formula (I) is 50.91%.
Repeat above-mentioned steps (1)-(5) 2 times, the content NDF (%) that calculates the forage rice neutral detergent fiber according to formula (I) is respectively 45.13% and 56.29%.
With the content NDF (%) of classical way mensuration forage rice neutral detergent fiber, the mensuration result of triplicate is respectively 51.82%, 46.28% and 56.17%; Can know that it is more accurate that method provided by the invention is measured the result.
Claims (10)
1. herbage neutral detergent fiber Determination on content method comprises the steps:
(1) is that the said herbage of A mixes to be placed on and preserves in the bottle with neutral cleansing solution and weight, said preservations bottle placed pressure cooker to be heated to seethe with excitement outgas;
(2) said preservation bottle is sealed the back with pressurizeing in the pressure cooker, obtain mixed liquor;
(3) use weight to carry out the said mixed liquor that filtration step (2) obtains as the filtrator of B, the filter cake that obtains is said neutral detergent fiber;
(4) with said filtrator and filtration cakes torrefaction to constant weight C, calculate according to formula (I) then and promptly get neutral detergent fiber content NDF (%) described in the then said herbage,
NDF(％)＝(C-B)/A×100％
(I)。
2. assay method according to claim 1 is characterized in that: herbage is powder described in the step (1), and the particle diameter of said powder is 18 orders-40 orders.
3. assay method according to claim 1 and 2 is characterized in that: said herbage is leguminous forage, graminous pasture or forage crop.
4. according to arbitrary described assay method among the claim 1-3, it is characterized in that: also comprise before the degassing described in the step (1) to the said step that adds AMS in the bottle of preserving.
5. according to arbitrary described assay method among the claim 1-4, it is characterized in that: said neutral cleansing solution is the mixed aqueous solution of lauryl sodium sulfate, sodium hydrogen phosphate and phosphoric acid.
6. according to arbitrary described assay method among the claim 1-5, it is characterized in that: the time of the degassing is 10 minutes-12 minutes described in the step (1).
7. according to arbitrary described assay method among the claim 1-6, it is characterized in that: the pressure of pressurization is 150kpa-170kpa described in the step (2), and the time of said pressurization is 40 minutes.
8. according to arbitrary described assay method among the claim 1-7, it is characterized in that: said filtrator is a filter paper, and the aperture of said filter paper is 80 μ m-120 μ m.
9. according to arbitrary described assay method among the claim 1-8, it is characterized in that: also comprise the step that said filter paper is dried to constant weight with micro-wave oven before filtering described in the step (3).
10. according to arbitrary described assay method among the claim 1-9, it is characterized in that: drying is carried out in micro-wave oven described in the step (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110092444.9A CN102735567B (en) | 2011-04-13 | 2011-04-13 | Method for determining neutral detergent fiber content in pasture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110092444.9A CN102735567B (en) | 2011-04-13 | 2011-04-13 | Method for determining neutral detergent fiber content in pasture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102735567A true CN102735567A (en) | 2012-10-17 |
| CN102735567B CN102735567B (en) | 2014-12-17 |
Family
ID=46991460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110092444.9A Expired - Fee Related CN102735567B (en) | 2011-04-13 | 2011-04-13 | Method for determining neutral detergent fiber content in pasture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102735567B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543084A (en) * | 2013-10-23 | 2014-01-29 | 浙江省纺织测试研究院 | Enzyme quantitative detection method of textile fiber content |
| CN108020483A (en) * | 2018-01-25 | 2018-05-11 | 杭州神农牧业有限公司 | Neutral detergent fiber measurement device |
| CN113627660A (en) * | 2021-07-30 | 2021-11-09 | 深圳市中金岭南有色金属股份有限公司凡口铅锌矿 | Method and device for predicting production rate of ceramic filter |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824851A (en) * | 2005-12-26 | 2006-08-30 | 苏州市利飞纺织品有限公司 | Preparation of original bamboo fiber by bioenzyme method |
-
2011
- 2011-04-13 CN CN201110092444.9A patent/CN102735567B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1824851A (en) * | 2005-12-26 | 2006-08-30 | 苏州市利飞纺织品有限公司 | Preparation of original bamboo fiber by bioenzyme method |
Non-Patent Citations (2)
| Title |
|---|
| 中华人民共和国国家质量监督检验检疫总局中国国家标准化管理委员会: "《中华人民共和国国家标准GB/T20806一200》", 30 December 2006, article "饲料中中性洗涤纤维(NDF)的测定", pages: 1-3 * |
| 朱顺国 等: "玉米秸秆NDF与ADF含量变化规律的研究", 《实验研究》, no. 1, 31 December 2001 (2001-12-31), pages 24 - 26 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543084A (en) * | 2013-10-23 | 2014-01-29 | 浙江省纺织测试研究院 | Enzyme quantitative detection method of textile fiber content |
| CN108020483A (en) * | 2018-01-25 | 2018-05-11 | 杭州神农牧业有限公司 | Neutral detergent fiber measurement device |
| CN113627660A (en) * | 2021-07-30 | 2021-11-09 | 深圳市中金岭南有色金属股份有限公司凡口铅锌矿 | Method and device for predicting production rate of ceramic filter |
| CN113627660B (en) * | 2021-07-30 | 2023-11-10 | 深圳市中金岭南有色金属股份有限公司凡口铅锌矿 | Method and device for predicting productivity of ceramic filter |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102735567B (en) | 2014-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104186835B (en) | A kind of maca leaf black tea and preparation method thereof | |
| Gul et al. | Proximate composition and mineral analysis of cinnamon | |
| CN103053808B (en) | Silage additive and preparation method and application thereof | |
| CN101209112A (en) | Eggshell calcium oxide powder and preparation and application thereof | |
| CN102735567B (en) | Method for determining neutral detergent fiber content in pasture | |
| Singh et al. | Study on dehydration of papaya slices using osmotic dehydration mediated hot air oven drying | |
| Nath et al. | Phytochemical and proximate analysis of papaya (Carica papaya) leaves | |
| CN110249997A (en) | It is suitable for the pollen suspension and the preparation method and application thereof of Kiwi berry liquid pollination | |
| CN104087533A (en) | Microbial agent for degrading kitchen wastes of naval vessels and ships and preparation method of microbial agent | |
| CN105613496A (en) | Quality modifier for improving red fruits, method for preparing quality modifier and application thereof | |
| Somers | The affinity of onion cell walls for calcium ions | |
| CN109652277A (en) | A kind of thorn pear wine and preparation method thereof rich in SOD | |
| CN102188463A (en) | Preparation method of hazel leaf extract | |
| CN103897049A (en) | Extraction method of Symphytum officinale leaf protein | |
| Durmaz et al. | An experimental study on extraction of sugar from carob using with taguchi method | |
| CN108562471B (en) | Method for rapidly detecting activity of plant root system | |
| CN102735566B (en) | Method for determining acid detergent fiber content in pasture | |
| CN107583699A (en) | A kind of heparin tube examined for clinical medicine and preparation method thereof | |
| Mlambo et al. | In vitro biological activity of tannins from Acacia and other tree fruits: Correlations with colorimetric and gravimetric phenolic assays | |
| CN102072903B (en) | Preparation method of sulfur dioxide standard sample used for food safety rapid detection box | |
| Thornton et al. | Copper uptake by ryegrass seedlings; contribution of cell wall adsorption | |
| Ayoub et al. | Physico-chemical analysis of fresh persimmon (Diospyros kaki L) fruit pulp from Jammu Region India | |
| CN103837426A (en) | Method for detecting silage mildew | |
| CN109805406A (en) | A kind of extracting method of rice bran combination phenol super-pressure-alkali-sour water solution substep release | |
| Mahendra et al. | Utilization of anthocyanin extract from rambutan fruit rind (Nephelium lappaceum l.) as an indicator of the quality on freshness meat |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141217 |