CN104316432A - Content measuring method of insoluble dietary fiber in food - Google Patents
Content measuring method of insoluble dietary fiber in food Download PDFInfo
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- CN104316432A CN104316432A CN201410642734.XA CN201410642734A CN104316432A CN 104316432 A CN104316432 A CN 104316432A CN 201410642734 A CN201410642734 A CN 201410642734A CN 104316432 A CN104316432 A CN 104316432A
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- buchner funnel
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- insoluble diedairy
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Abstract
The invention relates to a content measuring method of an insoluble dietary fiber in food. By the digestion action of a neutral detergent, substances such as sugar, starch, protein and pectin in a food sample are dissolved and removed, and indigestible residue is the insoluble dietary fiber which mainly comprises cellulose, hemicellulose, lignin, cutin, silica and the like, and further comprises insoluble ash. The method is convenient to implement and accurate in result.
Description
Technical field
The invention belongs to field of food detection, be specifically related to the content assaying method of insoluble diedairy fiber in a kind of food.
Background technology
Dietary fiber, refer to not easily digested dietary nutrient, the effect absorbing moisture content is had in digestive system, usually insoluble diedairy fiber and the large class of water-soluble dietary fiber two is divided into, it is the indispensable material of health diet, and insoluble diedairy fiber comprises cellulose, lignin and some hemicelluloses and from the wheat bran in food, corn bran, celery, pericarp and root vegetable.Non-soluble fiber can reduce the risk suffering from intestinal cancer, via middle noxious material Constipation and the diverticulitis of assimilating food, and can lower the toxin that in alimentary canal, bacterium is discharged simultaneously.
Summary of the invention
The object of the present invention is to provide the content assaying method of insoluble diedairy fiber in a kind of food.
The technical solution adopted for the present invention to solve the technical problems is: the content assaying method of insoluble diedairy fiber in a kind of food, comprises the following steps:
1) extract three times with 10mL sherwood oil after being pulverized by 1g food samples and slough fat, add 100mL neutral detergent solution, 5 n-octyl alcohols and 0.5g anhydrous sodium sulfite, be then placed in heating on electric furnace and boil, move to micro-1h that boils on electric hot plate;
2) in Buchner funnel middle berth 1g glass wool, move in baking oven, 110 DEG C of 4h, take out and put in exsiccator, dry in the air to room temperature, ten thousand/balance is weighed to obtain m
1;
3) pour the sample after boiling into Buchner funnel suction filtration while hot, with 90-100 DEG C of hot wash then suction filtration to dry, clean liquid and foam, the beyond the Great Wall rubber stopper of filter bottom;
4) in Buchner funnel, add 2.5% amylase liquid, liquid level need cover fiber, covers table glass ware, hydrolyse over night in 37 DEG C of constant temperature ovens;
5) take out Buchner funnel, removing bottom stopper, pumps amylase liquid, and divides several to wash away residual enzyme liquid with 300mL hot water, uses iodine fluid inspection, if any residual, continue to add enzyme hydrolysis, as starch eliminates, drain, then wash 2 times with acetone;
6) put in baking oven by Buchner funnel, 110 DEG C of 4h, take out, put in exsiccator, dry in the air to room temperature, accurately weigh to obtain m
2, be then calculated as follows insoluble diedairy fiber content:
Wherein, C---the content of insoluble diedairy fiber in sample, %;
M
1---Buchner funnel adds the quality of glass wool, g;
M
2---Buchner funnel adds the quality of fiber in glass wool and sample, g;
M---sample quality, g.
The preparation steps of described neutral detergent solution is: 18.61gEDTA disodium salt and 6.81g sodium borate decahydrate are placed in beaker, add water about 150mL, heating makes it to dissolve, 30g NaLS and 10mL ethylene glycol monoethyl ether are dissolved in about 700mL hot water, merge above-mentioned two liquid, then 4.56g ADSP is dissolved in 150mL hot water, then be incorporated in above-mentioned solution, regulate above-mentioned mixed liquor to pH6.9-7.1 with phosphoric acid, finally add water to 1000mL.
The preparation steps of described amylase liquid is: take 2.5g diastase and be dissolved in the phosphate buffered solution of 100mLpH7, centrifugal, and filter, the amylase liquid of filtration is for subsequent use.
The present invention has following beneficial effect: the present invention measures the content of insoluble diedairy fiber in food under the digestion of neutral detergent, the materials such as the sugar in food samples, starch, protein, pectin are dissolved removing, the residue that can not digest is insoluble diedairy fiber, mainly comprise cellulose, hemicellulose, lignin, cutin and silicon dioxide etc., and comprise insoluble ash content, method is easy to operate, and result is accurate.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is described further, but protection scope of the present invention is not limited to these embodiments.Every do not deviate from the present invention's design change or equivalent substituting include within protection scope of the present invention.
A content assaying method for insoluble diedairy fiber in food, comprises the following steps:
1) extract three times with 10mL sherwood oil after being pulverized by 1g food samples and slough fat, add 100mL neutral detergent solution, 5 n-octyl alcohols and 0.5g anhydrous sodium sulfite, be then placed in heating on electric furnace and boil, move to micro-1h that boils on electric hot plate; The preparation steps of described neutral detergent solution is: 18.61gEDTA disodium salt and 6.81g sodium borate decahydrate are placed in beaker, add water about 150mL, heating makes it to dissolve, 30g NaLS and 10mL ethylene glycol monoethyl ether are dissolved in about 700mL hot water, merge above-mentioned two liquid, then 4.56g ADSP is dissolved in 150mL hot water, then be incorporated in above-mentioned solution, regulate above-mentioned mixed liquor to pH6.9-7.1 with phosphoric acid, finally add water to 1000mL.
2) in Buchner funnel middle berth 1g glass wool, move in baking oven, 110 DEG C of 4h, take out and put in exsiccator, dry in the air to room temperature, ten thousand/balance is weighed to obtain m
1.
3) pour the sample after boiling into Buchner funnel suction filtration while hot, with 90-100 DEG C of hot wash then suction filtration to dry, clean liquid and foam, the beyond the Great Wall rubber stopper of filter bottom.
4) in Buchner funnel, add 2.5% amylase liquid, liquid level need cover fiber, covers table glass ware, hydrolyse over night in 37 DEG C of constant temperature ovens; The preparation steps of described amylase liquid is: take 2.5g diastase and be dissolved in the phosphate buffered solution of 100mLpH7, centrifugal, and filter, the amylase liquid of filtration is for subsequent use.
5) take out Buchner funnel, removing bottom stopper, pumps amylase liquid, and divides several to wash away residual enzyme liquid with 300mL hot water, uses iodine fluid inspection, if any residual, continue to add enzyme hydrolysis, as starch eliminates, drain, then wash 2 times with acetone.
6) put in baking oven by Buchner funnel, 110 DEG C of 4h, take out, put in exsiccator, dry in the air to room temperature, accurately weigh to obtain m
2, be then calculated as follows insoluble diedairy fiber content:
Wherein, C---the content of insoluble diedairy fiber in sample, %;
M
1---Buchner funnel adds the quality of glass wool, g;
M
2---Buchner funnel adds the quality of fiber in glass wool and sample, g;
M---sample quality, g.
Claims (3)
1. the content assaying method of insoluble diedairy fiber in food, is characterized in that, comprise the following steps:
1) extract three times with 10mL sherwood oil after being pulverized by 1g food samples and slough fat, add 100mL neutral detergent solution, 5 n-octyl alcohols and 0.5g anhydrous sodium sulfite, be then placed in heating on electric furnace and boil, move to micro-1h that boils on electric hot plate;
2) in Buchner funnel middle berth 1g glass wool, move in baking oven, 110 DEG C of 4h, take out and put in exsiccator, dry in the air to room temperature, ten thousand/balance is weighed to obtain m1;
3) pour the sample after boiling into Buchner funnel suction filtration while hot, with 90-100 DEG C of hot wash then suction filtration to dry, clean liquid and foam, the beyond the Great Wall rubber stopper of filter bottom;
4) in Buchner funnel, add 2.5% amylase liquid, liquid level need cover fiber, covers table glass ware, hydrolyse over night in 37 DEG C of constant temperature ovens;
5) take out Buchner funnel, removing bottom stopper, pumps amylase liquid, and divides several to wash away residual enzyme liquid with 300mL hot water, uses iodine fluid inspection, if any residual, continue to add enzyme hydrolysis, as starch eliminates, drain, then wash 2 times with acetone;
6) put in baking oven by Buchner funnel, 110 DEG C of 4h, take out, put in exsiccator, dry in the air to room temperature, accurately weigh to obtain m2, is then calculated as follows insoluble diedairy fiber content:
Wherein, the content of insoluble diedairy fiber in C-sample, %;
M1-Buchner funnel adds the quality of glass wool, g:
M2-Buchner funnel adds the quality of fiber in glass wool and sample, g;
M-sample quality, g.
2. the content assaying method of insoluble diedairy fiber in food according to claim 1, it is characterized in that, the preparation steps of described neutral detergent solution is: 18.61gEDTA disodium salt and 6.81g sodium borate decahydrate are placed in beaker, add water about 150mL, heating makes it to dissolve, 30g NaLS and 10mL ethylene glycol monoethyl ether are dissolved in about 700mL hot water, merge above-mentioned two liquid, again 4.56g ADSP is dissolved in 150mL hot water, be incorporated in above-mentioned solution again, regulate above-mentioned mixed liquor to pH6.9-7.1 with phosphoric acid, finally add water to 1000mL.
3. the content assaying method of insoluble diedairy fiber in food according to claim 1, it is characterized in that, the preparation steps of described amylase liquid is: take 2.5g diastase and be dissolved in the phosphate buffered solution of 100mLpH7, centrifugal, filter, the amylase liquid of filtration is for subsequent use.
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| CN201410642734.XA CN104316432A (en) | 2014-11-14 | 2014-11-14 | Content measuring method of insoluble dietary fiber in food |
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| CN201410642734.XA CN104316432A (en) | 2014-11-14 | 2014-11-14 | Content measuring method of insoluble dietary fiber in food |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106092815A (en) * | 2016-06-27 | 2016-11-09 | 江西出入境检验检疫局检验检疫综合技术中心 | The assay method of total dietary fiber in a kind of food |
| CN106124358A (en) * | 2016-07-07 | 2016-11-16 | 上海交通大学 | A kind of measure the method washing fiber content in sweet sorghum stalk residue |
| CN115521479A (en) * | 2022-09-15 | 2022-12-27 | 上海应用技术大学 | Preparation method of lignin derivative |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539274A (en) * | 2012-01-11 | 2012-07-04 | 山东农业大学 | Fiber screen bag and method for rapidly determining coarse fiber |
| CN203658137U (en) * | 2013-12-25 | 2014-06-18 | 上海纤检仪器有限公司 | Detecting device of dietary fibers |
| CN203811498U (en) * | 2014-01-20 | 2014-09-03 | 宜宾学院 | Cellulose measuring device |
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2014
- 2014-11-14 CN CN201410642734.XA patent/CN104316432A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539274A (en) * | 2012-01-11 | 2012-07-04 | 山东农业大学 | Fiber screen bag and method for rapidly determining coarse fiber |
| CN203658137U (en) * | 2013-12-25 | 2014-06-18 | 上海纤检仪器有限公司 | Detecting device of dietary fibers |
| CN203811498U (en) * | 2014-01-20 | 2014-09-03 | 宜宾学院 | Cellulose measuring device |
Non-Patent Citations (2)
| Title |
|---|
| 潘铎: "谷物和谷物食品中不溶性膳食纤维的测定", 《营养学报》 * |
| 赵忠林: "《中华人民共和国国家标准》", 1 January 2004 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106092815A (en) * | 2016-06-27 | 2016-11-09 | 江西出入境检验检疫局检验检疫综合技术中心 | The assay method of total dietary fiber in a kind of food |
| CN106124358A (en) * | 2016-07-07 | 2016-11-16 | 上海交通大学 | A kind of measure the method washing fiber content in sweet sorghum stalk residue |
| CN115521479A (en) * | 2022-09-15 | 2022-12-27 | 上海应用技术大学 | Preparation method of lignin derivative |
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