CN106119116A - The single cell protein compound enzymic preparation utilizing food and drink to produce waste water cultivation prepares the method for yeast autolysate and made yeast product - Google Patents

Abstract

The invention provides a kind of method that single cell protein compound enzymic preparation utilizing food and drink to produce waste water cultivation prepares yeast autolysate, it is to carry out mixing self-dissolving then dry, sterilizing in the ratio of dry biomass (2:8)～(8:2) with yeast with unicellular compound enzymic preparation, self-dissolving is divided into low-temperature zone and high temperature section, high temperature section keeps 30～120 minutes at 75～95 DEG C, and described unicellular compound enzymic preparation is that food and drink production waste water is obtained by following steps: (1) produces waste water to food and drink and carries out pretreatment；(2) pretreated food and drink is produced waste water and carry out nutrient substance regulation；(3) microorganism culturing；(4) bacterium mud is collected and is obtained unicellular compound enzymic preparation.The present invention has important environment protection significance, and has great economic worth.

Description

The single cell protein compound enzymic preparation utilizing food and drink to produce waste water cultivation prepares ferment The method of female autolysate and made yeast product

Technical field

The present invention relates to single cell protein produce and the technical field of application.

Background technology

Single cell protein (Single cell protein is called for short SCP), also known as microprotein or tropina, is to utilize Industrial wastewater, waste gas, natural gas, petroleum alkane class, agricultural and sideline converted products and organic waste etc., as culture medium, cultivate ferment The unicellular microorganism such as pathogenic antibacterial female, non-, miniature bacterium, fungus.Single cell protein is not a kind of true protein, but by egg The mixture groups such as white matter, fat, carbohydrate, nucleic acid and the nitrogen-containing compound of non-protein class, vitamin and inorganic compound The Cytoplasm group become.Important in single cell protein have yeast protein, bacterioprotein and algae protein, their chemical group Typically based on protein, fat in one-tenth.

The nutrient substance such as albumen contained by single cell protein, aminoacid are all right in addition to may be directly applied to protein sources Its contained endogenous enzymes is applied to carry out deep processing.Endogenous enzymes contained by single cell protein generally comprise lysozyme, protease and Nuclease, these enzymes both can carry out extraction can also carry out self-dissolving to produce the high-value products such as little peptide, nucleotide.

Some single cell proteins such as bacillus subtilis, the Bacillus licheniformis etc. beyond yeast is used at biological field Extract the existing highly developed application of certain enzyme preparation, from bacillus subtilis and aspergillus oryzae, such as extract xylanase, from Bacillus lentus extracts mannase, from Bacillus licheniformis, extracts amylase.This instruction sheet cell protein has Very abundant multiple endogenous enzymes can extract the application of industry of doing business of going forward side by side.

Most of enzyme of industrial use comes from the extraction of single cell protein, and extracts the process activity product always to enzyme Raw negative effect, if it can be considered to the single cell protein containing endogenous enzymes of directly using carry out as the enzyme source of crude product Commercial production may have good effect.It appeared that the single cell protein kind apoplexy due to endogenous wind in addition to yeast has a lot of thalline Abundant endogenous enzymes can be contained, and the kind of these endogenous enzymes and activity are all that yeast autolysis process needs especially, as Nuclease can be with the RNA of enzymatic yeast, and lysozyme then can improve yeast sporoderm-broken rate.It is therefore contemplated that if, with The single cell protein that endogenous enzymes is abundant may improve the efficiency of yeast autolysis if carrying out self-dissolving together with yeast.

The microbial treatments of waste water refer to the micropopulation utilizing domestication to cultivate, at the aeration environment of artificial-strengthening Middle suspension growth, decomposes biodegradable organic substance in oxidized waste water so that the method that waste water is purified.According to process The difference of technique, the growth conditions of micropopulation is the most different, suspension growth be called activated sludge, be attached on filler raw Long is called microbial film.This micropopulation, mainly includes antibacterial, protozoacide and algae.Wherein antibacterial is in quantity and matter Have comparative advantage in amount, account for the 90%～more than 94% of micropopulation quality.

Yeast is widely used in the fields such as bread fermentation, beer fermentation, animal feed, and it is of a great variety, including bread ferment Mother, beer yeast, molasses yeast etc..A lot of existing food materials, such as molasses, corn starch, corn syrup and Radix Betae etc., All can turn out substantial amounts of yeast by the way of fermentation.

Yeast autolysis technology production yeast autolysate, yeast extract, food dressing is utilized the most well to apply, self-dissolving Purpose obtains the higher product of value exactly, it is thus achieved that the higher Dissolve things inside of nutritive value, can produce free nucleoside after yeast autolysis Peptide sour, little and free amino acid etc., nucleotide has significant delicate flavour and immunologic function, such as yeast hydrolyate, it is simply that refer to ferment Mother is strain, through the thalline that liquid fermentation obtains, concentrates or be dried the product obtained after self-dissolving or exogenous enzyme catalyzing hydrolysis.Ferment Female hydrolysate has more preferable nutritive value than original yeast in Animal nutrition field.Therefore, how to improve self-dissolving efficiency to carry After high self-dissolving, the value of product just becomes a major technique research direction.Existing industrial circle is to this existing more research and answers With.

The production method of the yeast extract of a patent of invention CN101513247B[high protein content and product] public Having opened a kind of method using self-dissolving technology to produce yeast extract, extract Autolysis Condition disclosed herein is mainly pH and keeps 4～7, temperature is maintained at 45～55 DEG C, response time 3～12 hours.And need to add autolysis promoter and exogenous enzyme.It is certainly Molten enzymolysis and two steps of temperature reaction of including, time-consuming 5～30 hours respectively, and 3～10 hours.And in description and enforcement Example describes the manufacturing parameter that autolytic process is the most longer.

Patent of invention CN102051381B[mono-kind utilizes the method that beer yeast produces yeast extract], the self-dissolving of introduction The parameter of method is: use 55～65 DEG C of self-dissolvings 20～50 hours in the presence of papain.

Patent of invention CN101686720A[yeast autolysate] disclose the side that another kind of use self-dissolving technology carries out producing Method, disclosed main self-dissolving parameter includes: in the case of temperature is maintained at 30～70 DEG C, uses outer interpolation protease to carry out self-dissolving, Self-dissolving was for up to 20 hours.

Patent of invention CN102293356A[yeast autolysate and preparation method thereof] disclose the most concrete a kind of yeast Autolysate preparation method, involved Autolysis Condition is temperature 50～70 DEG C, and pH4.5～7.0 adds 0.1%-1.0% enzyme Preparation.The self-dissolving time that refer to such parameter in an embodiment needs has reached 10～28 hours.

Patent of invention CN101050427A[mono-kind produces the preparation method of Ruan's candida mycoderma autolysate], describe a kind of use Yeast autolysate is as the production method of flavoring, and its main Autolysis Condition is pH4.5～6.8, temperature range 45～ 55 DEG C, add the flavouring agent such as sodium chloride, yellow wine, stirring self-dissolving 24 hours.

Patent of invention CN101779722B[yeast culture and compounding method thereof] in yeast culture main from Molten parameter be then 50～60 DEG C under the conditions of self-dissolving 30～40 hours.

Patent of invention CN102031275A[utilizes the method that waste yeast prepares yeast extract], carry when introducing extraction process To two kinds of methods, one is enzyme process extracting, is incubated enzymolysis 8～24 hours at 45～55 DEG C in the case of adding specific extracting enzyme； Two be self-dissolving extracting be then under the conditions of 48～55 DEG C be incubated 24-48 hour.

Above-mentioned patent is all to use self-dissolving to realize the lifting that yeast is worth, it is therefore an objective to obtain the Dissolve things inside of yeast, nucleoside Peptide sour, little, flavor substance, immune material and some other high value nutrient, and this is applied to Animal nutrition, microorganism training Foster, food additive.In method disclosed in these patents, the mode of production before and after self-dissolving is had nothing in common with each other, but the parameter of self-dissolving technique There are some common ground:

The parameter that yeast autolysis is suitable for is about temperature in the range of 30～70 DEG C, and the scope more refined includes: 45～55 DEG C, 50～60 DEG C, 50～70 DEG C etc..

Time needed for another common ground of above-mentioned patent is self-dissolving is the most relatively long, and from the point of view of comprehensive literature, required time exists 3～50 hours, in the case of not using exogenous enzyme and autolysis promoter, the general time needed was about 24～48 hours, outside using Within source enzyme or the situation self-dissolving time such as surface promoter or surfactant can foreshorten to 24 hours, even 12 hours with In.Although within the time is the most significantly contracted to 12 hours from the prior art, but this is to use various external means Realize, it appears that yeast itself relies on the endogenous enzymes self-dissolving of self-dissolving need nonetheless remain for the longer time and be difficult to reach industrial high-efficient The requirement produced.

From the point of view of commercial production angle, low temperature and time-consuming length that main two features of existing self-dissolving technology are relative can cause A lot of problems produce:

Low temperature state can cause putrefactive microorganisms to grow, because the existing temperature being all less than pasteurization from solubility temperature (68～70 DEG C), say, that when material is when less than 68 DEG C, the growth of putrefactive microorganisms while self-dissolving, can be attended by.Not Use in the case of exogenous enzyme, start putrefactive microorganisms from self-dissolving and can carry out growing with self-dissolving and can be with exponential life always Long, in the case of using exogenous enzyme, although self-dissolving initial stage autolytic enzyme can putrefactive microorganisms always, but on enzymolysis later stage antibacterial Still may proceed to grow, therefore, all can be it may happen that the situation of putrefactive microorganisms raised growth occurs in the self-dissolving later stage, this will be right Produce control and cause puzzlement.

Low temperature and long-time self-dissolving can produce the harmful substance of high level, including harmful base, inorganic nitrogen and biogenic amine, Harmful microbe growth inevitably results in the generation of harmful substance, and the nutrient substance of high value can reduce accordingly simultaneously, as The nucleotide that self-dissolving produces can be utilized and produce base by microorganism, and aminoacid then microorganism can be converted into inorganic nitrogen.Thus shadow Ring final products quality, nutritive value and safety.

The material disposal ability that time-consuming longer but front and back the operation of self-dissolving operation is general is strong, and this is accomplished by designing Large Copacity Self-dissolving equipment, the most jumbo enzymatic vessel and reactor etc., this can cause cost increase.

From the point of view of existing disclosed self-dissolving technology, all employ exogenous enzyme in most cases and autolysis promoter carries High self-dissolving efficiency, the interpolation of exogenous enzyme can significantly improve again production cost.

Add in the case of exogenous enzyme will to self-dissolving time material moisture content have specific requirement, in most cases Needing higher moisture content and have the highest mobility, this could improve the efficiency of exogenous enzyme.And this can improve follow-up The cost being dried.

From the shortcoming of foregoing description and the data of offer it can be seen that compare yeast certainly with the high efficiency of modern enzymolysis process Molten the most much lower as its efficiency of enzymolysis process, reason is that the endogenous enzymes that yeast itself is had is not enriched, thus leads Cause needs to use external means to carry out self-dissolving strengthening, and this includes the prolongation time, improves outside moisture content, the parameters such as pH of keeping under strict control, interpolation Source enzyme.

In the most existing disclosed technology, in the case of using exogenous enzyme and autolysis promoter, the time of self-dissolving is still The longest, it can be seen that for cost reasons, exogenous enzyme cannot add in a large number, the addition mentioned in existing public technology For efficiency the highest.

Therefore, new thinking is used will to have very important significance to the self-dissolving efficiency improving yeast.

Summary of the invention

It is an object of the invention to solve the defect of existing yeast autolysis inefficiency, it is provided that a kind of efficient, rich in activity The production method of the abundant single cell protein of the use endogenous enzymes of composition and yeast mixing self-dissolving.

To achieve these goals, present invention employs techniques below scheme: utilize food and drink to produce what waste water was cultivated Single cell protein compound enzymic preparation prepares the method for yeast autolysate, is by dry matter with unicellular compound enzymic preparation and yeast The ratio of amount (2:8)～(8:2) carries out mixing self-dissolving then dry, sterilizing, and self-dissolving is divided into low-temperature zone and high temperature section, and low-temperature zone exists 45～60 DEG C keep 20～60 minutes, and high temperature section keeps 30～120 minutes at 75～95 DEG C,

Described unicellular compound enzymic preparation is that food and drink production waste water is obtained by following steps:

(1) food and drink producing waste water and carry out pretreatment, slagging-off oil removing also adjusts pH to 5～9, and pretreated food is drunk Material produce waste water BOD concentration between 100-5000mg/L, described food and drink produce waste water include starch or sugar or Beverage or canned food or meat or Aquatic product or wine brewing or rice product or milk product or bean product or fermented seasonings Production phase water, described production phase use water include food and drink raw material soaking, germinate, pulverize, squeeze, extract, dilute, Concentrate, cleaning waste water that steaming and decocting produces during frying, or saccharifying, the filtration fermenting, remove the gred, separate, produce in purification process In waste water, or packaging process evaporating, emitting, dripping or leaking of liquid or gas finished product and clean produce waste water.；

(2) pretreated food and drink is produced waste water and carry out nutrient substance regulation, make to supplement after nitrogen, phosphorus in waste water BOD: total nitrogen: total phosphorus=100:(5-20): (0.5-2)；

(3) using the usual aerobic activity flora of environmental protection industry (epi) as the production thalline of unicellular compound enzymic preparation, described multiple The thalline that produces of synthase preparation includes the one from hair zygosaccharomyces, Micrococcus, corynebacterium, bacillus, will The production thalline of compound enzymic preparation is inoculated into aeration culture pond, and the waste water after step (2) nutrient substance regulates pumps into aeration training Supporting pond, be sufficiently mixed with inoculation thalline, thalline is cultivated in the breeding of aeration culture pond, and omnidistance aeration oxygen replenishing ensures that aeration culture pond is molten Solution oxygen is 2～4mg/L, and temperature is limited to 10～40 DEG C, and pH is limited to 6.5～8.5, and thalline breeding obtains unicellular compound enzyme after cultivating Preparation, unicellular compound enzymic preparation precipitates with flock and microbial film form and exists with bacterium pureed state；

(4) bacterium mud is collected and is obtained unicellular compound enzymic preparation.

When food and drink production waste water is brewing wastewater, the production equipment of unicellular compound enzymic preparation includes setting according to operation The filter plant put, regulating reservoir, PH regulate tank, preliminary sedimentation tank, Aeration tank, second pond, the holding vessel of tool aeration performance, production stage Include successively:

(1) brewing wastewater pretreatment: using centrifuge, flame filter press and grid maker as filter plant, containing diatomite Waste water machine by centrifugation is centrifuged, and the waste water containing yeast filters through board and frame machine, jointly filters through grid maker with other malting effluent, filters After Waste Water Centralized in regulating reservoir, be then pumped into pH regulator tank, regulate pH to 7；

(2) waste water regulating pH is pipelined to preliminary sedimentation tank, and the waste residue after precipitation is discharged bottom preliminary sedimentation tank, on Clear liquid flows out from preliminary sedimentation tank top layer overfall, is respectively mounted COD, total nitrogen, total phosphorus online detection instrument in preliminary sedimentation tank overflow outlet, According to BOD: total nitrogen: total phosphorus=100:8:1, wherein BOD=COD*0.55, calculate the magnitude of recruitment of total nitrogen, total phosphorus,

Using carbamide and potassium dihydrogen phosphate as nitrogen source and the supplementary source of phosphorus source, the aqueous solution being configured to fixed concentration adds In waste water；

Using zinc sulfate and manganese sulfate as the supplementary interpolation of trace element, add concentration and press 2mg/L Zn in waste water²⁺With 1mg/L Mn²⁺Concentration necessary be added；

(3) microbial culture method of brewing wastewater selects continuous culture method, and thalline breeding training method selects the most mixed Box-like, brewing wastewater is delivered to Aeration tank from preliminary sedimentation tank through pipeline,

Bacterium mud and brewing wastewater are sufficiently mixed at Aeration tank, and in Aeration tank breeding incubation, omnidistance aeration oxygen replenishing ensures Culture pond dissolved oxygen is between 2～4mg/L, and pH is between 7.5～8.5, and Aeration tank is open culture environment, and thalline is with cotton-shaped Form be blended in Aeration tank, thalline mixed liquor delivers into two from Aeration tank end overflow, thalline mixed liquor by pipeline Heavy pond, relies on action of gravity at second pond, and cotton-shaped bacterial sediment gets off, and forms bacterium mud and discharges bottom second pond, a part The holding vessel entering tool aeration performance is used for producing unicellular compound enzymic preparation, and another part is transmitted back to aeration as backflow bacterium mud Pond,

(4) the bacterium mud in holding vessel is processed into unicellular compound enzymic preparation product through precipitation, concentration.

Enter the bacterium mud amount produced for unicellular compound enzymic preparation of holding vessel of tool aeration performance according to Aeration tank temperature Degree determines, when Aeration tank temperature is at 10～20 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation accounts for the total bacterium of Aeration tank The 1/13～1/15 of mud amount；When Aeration tank temperature is at 20～30 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation accounts for The 1/11～1/13 of Aeration tank total bacterium mud amount；When Aeration tank temperature is at 30～40 DEG C, produce for unicellular compound enzymic preparation Bacterium mud amount account for the 1/8～1/11 of Aeration tank total bacterium mud amount.

When food and drink production waste water is amino acids production waste water, the production equipment of unicellular compound enzymic preparation includes depending on The filter plant of operation setting, regulating reservoir, preliminary sedimentation tank, sump, SBR aeration culture pond, the holding vessel of tool aeration performance, produce Step includes successively:

(1) amino acids production Wastewater Pretreatment: using grid maker as filter plant, waste water pump is delivered to grid maker and is filtered to go Except suspension oarse-grained in waste water, floating thing, grid pitch selects 3mm, and the waste water after filtration is delivered to regulating reservoir through pipeline, and pH adjusts Saving between 6-8, regulating reservoir waste water pumps into preliminary sedimentation tank, and precipitation waste residue is collected and removed, and supernatant is defeated in sedimentation tank end overflow Deliver to sump；

(2) sump contain one for stirring immersible pump, take sump water sample detection TOC, total nitrogen, total phosphorus, according to TOC: total nitrogen: total phosphorus=100:12:1.5, calculates the magnitude of recruitment of total nitrogen, total phosphorus, with ammonium nitrate and calcium superphosphate for nitrogen source and The supplementary source of phosphorus source, disposably adds in sump；

(3) microbial culture method of amino acids production waste water selects the SBR technique of batch-type culture method, thalline breeding training Foster mode selects to be thoroughly mixed formula, and the waste water in sump enters SBR aeration culture pond after the dilution of dilution water, exposes at SBR Gas culture pond completes, into water, aeration, precipitation, draining, discharge of bacteria mud overall process, to continue aeration in fill phase according to time sequencing, Aeration ensures that DO is 0.8～1.2mg/L；Being thoroughly mixed at aeration phase amino acids production waste water and bacterium mud, thalline is with cotton-shaped Form is blended in SBR aeration culture pond, and this stage whole process aeration oxygen replenishing ensures that SBR aeration culture pond dissolved oxygen is 1～2mg/L Between, pH is between 7.5～8.5, and SBR aeration culture pond is open culture environment, and cultivation temperature is limited to 10～40 DEG C；Stop After aeration, cotton-shaped bacterial sediment gets off, and forms bacterium mud；Draining and spoil disposal stage are carried out simultaneously, and spoil disposal refers to that bacterium mud is trained from SBR aeration Support and discharge bottom pond, produce for unicellular compound enzymic preparation, stay part bacterium mud to stay SBR Aeration tank conduct as inoculation bacterium mud Inoculation liquid is standby；

(4) the bacterium mud being used for producing unicellular compound enzymic preparation discharged from SBR aeration culture pond is collected in tool aeration merit In the holding vessel of energy, holding vessel, at the dissolved oxygen ＞ 2mg/L of the aeration guarantee bacterium mud of collection phase, stops after having collected storing The aeration of tank and stirring, bacterium mud prepares unicellular compound enzymic preparation through precipitation, concentration.

The bacterium mud amount produced for unicellular compound enzymic preparation discharged from SBR aeration culture pond is cultivated according to SBR aeration Pond temperature determines, when SBR aeration culture pond temperature is at 10～20 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation accounts for The 1/11～1/12 of SBR aeration culture pond total bacterium mud amount；When SBR aeration culture pond temperature is at 20～30 DEG C, for unicellular The bacterium mud amount that compound enzymic preparation produces accounts for the 1/9～1/10 of SBR aeration culture pond total bacterium mud amount；When SBR aeration culture pond temperature

The parameter of unicellular compound enzymic preparation is as follows: butt albumen 40.0%～60.0%；Butt total amino acids 35.0% ～55.0%；Butt inorganic nitrogen 0～5.0%；Butt total nucleotide 2.0%～6.0%；Total nucleotide/total amino acids ratio 0.07～0.12；Total nucleotide/albumen 0.05～0.10；Antalzyme activity 150-500U/g butt；Nuclease 500-4000U/g Butt；Protease 1000-10000U/g butt.

Unicellular compound enzymic preparation is (3:7)～(7:3) with the mixed proportion of yeast, the most specific unicellular compound enzyme system Agent is 1:1 with the mass content ratio of yeast.

Described yeast includes one or more the mixing in beer yeast, bakery yeast, molasses yeast, and yeast Without beer yeast concentrated solution and the yeast paste of nucleic acid extraction technique, preferably undried, doing of described beer yeast Material protein content is 40%～55%.

Another object of the present invention is to provide a kind of by the yeast product obtained by any of the above-described described method.

Biodiversity content in above-mentioned yeast product is 5.0%-10.0%, free nucleotide mass content is 1.40%-3.00%, free alkali base mass content are 0-0.20%, albumen quality content is 40.0%-60.0%, wherein albumen In little peptide mass content be 15.00%～40.00%, free amino acid mass content in albumen be 2.0%-8.0%.

Compared with the existing technology, raw material, production technology and the product made thereof have the advantage that the present invention

Low using food and drink waste water as cost of material, reduce the production cost of single cell protein compound enzymic preparation.Useless Containing substantial amounts of nutrient in water, thus decrease the input of Exogenous Nutrients (nitrogen source, phosphorus source etc.).In raw material, Dissolved Organic Matter contains Amount height, remains matrix content and reduces in the enzyme preparation prepared, the purity of enzyme is improved.Raw material biological safety is high, in waste water Biodegradable composition is many, is natural organic matter matter, without poisonous and harmful substance.Raw material organism kinds is many, and microorganism is fitted The multiple enzyme of output of answering property organic is assimilated for these.Raw material organic concentration and existing micro-organism enzyme preparation Raw materials for production are compared much lower, and microorganism adaptive output extracellular polymeric is used for adsorbing organic substance, extracellular polymeric In containing multiple exoenzyme, larger molecular organics is resolved into small organic molecule, it is simple to rapidly diffuse into intracellular.Raw material comes Source is extensive, produces sustainable.

Food and drink waste water is cultivated the technique of unicellular compound enzymic preparation and is had the advantage that

Now, the production method of micro-organism enzyme preparation generally uses permanent turbid culture method, after incubation terminates, also has big The culture of amount remains in culture fluid, is completely mixed together with microbial cells.These culture materials mostly do not contain enzyme, with Microbial cells mixes the concentration reducing enzyme, in order to improve the content of enzyme in product, needs further extracting and developing Etc. step, and can the activity reducing enzyme in various degree during extracting and separating.The processing procedure of waste water, belongs to permanent Change culture method, say, that during micropopulation growth and breeding, nutrient substance is restrictive factor.General, waste water Middle BOD concentration is difficult to be absorbed by micropopulation less than 20mg/L again.Micropopulation is inhaled completely by metabolism Receiving and converted the organic substance in waste water, activated sludge or the microbial film almost all of formation are microbial cells.Permissible Directly utilize microbial cells as enzyme preparation product.

The enzyme preparation comparison of ingredients that the production method of existing micro-organism enzyme preparation is produced is single, for albumen feedstuff The addition of enzymolysis often enzyme is very big, and enzymolysis efficiency is low, and the taste of enzymolysis product is poor.Used in the present invention is bacterium Group can produce several functions enzyme, including protease, nuclease and autolytic enzyme.These enzymes act in albumen feedstuff enzymolysis process Substrate diverse location, substantially increases enzymolysis efficiency.Meanwhile, the zymolysis process of these enzymes is different from industrial enzyme, will not produce Raw bitter peptides, remains the local flavor of albumen feedstuff.

The unicellular compound enzymic preparation that patent of the present invention provides produce do not have the buying of raw material, transport, load and unload, proportioning；Not yet There is the control of cultivation temperature, decrease heat-insulation system operation；Bacterium mud, directly as unicellular compound enzymic preparation raw material, does not has cell The technique such as breaking cellular wall, extraction.Greatly reduce the difficulty of production operation.

The production of existing micro-organism enzyme preparation generally has waste residue, waste liquid produces, discharge.Patent of the present invention is producing list While cell compound enzymic preparation, the Organic substance in food and drink waste water is consumed totally, and waste water becomes clear water and can directly arrange Put, alleviate carrying capacity of environment.

Owing to the concentration ratio of food and drink waste water is relatively low, the microorganism species concentration cultivating generation is the lowest, general, The mass concentration of aeration microorganism is at 2000mg/L～6000mg/L.But, these microorganism species form flock, at aeration Relying on action of gravity to precipitate in sedimentation tank after pond, mass concentration can reach more than 30000mg/L (moisture content 97% Below), being then dehydrated further by additional flocculating agents, moisture content can be reduced to 80%～84%.Reach and chemostat cultivation The equal moisture content level of method, it is ensured that production cost will not increase.

Micropopulation that food and drink waste water processes is utilized to have a following advantage as enzyme preparation:

Enzyme preparation safety is high, and food and drink waste water does not contains poisonous and harmful substance.

Enzyme preparation activity is high；The ferment made especially with the unicellular compound enzymic preparation for yeast enzymolysis of the present invention Female product improves further due to effective ingredient such as nucleotide so that the palatability of product, abnormal smells from the patient obtain bigger reinforcement.Nucleoside Acid content raising also contributes to product has had bigger effect in terms of improving animal immune and improving production performance.And existing therefore, There are yeast autolysate, hydrolysate to compare when being applied to animal feed and just have preferably performance.

Enzyme preparation plyability is good, and the unicellular compound enzymic preparation for yeast enzymolysis especially with the present invention can make The nucleotide content of yeast can obtain bigger lifting than prior art, and the free nucleotide of product, little peptide, free amino acid are wanted It is significantly higher than existing yeast autolysate.

Enzyme preparation purity is high, can directly utilize microbial cells as enzyme preparation；Being used for especially with the present invention Final products, owing to addition is high, be contribute to the most again a part of nutrition by the unicellular compound enzymic preparation of yeast enzymolysis Point, also contribute to the protein ingredient of significant proportion, thus also improve the yield of final products.

Enzyme preparation production cost is low, and the particularly cost of product also to significantly reduce with existing yeast product, so that Obtain yeast product and can have larger range of application.

The direct of present invention innovation will be applied to yeast autolysis containing the single cell protein of abundant endogenous enzymes as exogenous enzyme, The endogenous enzymes comparing single cell protein with the industrial enzymes of common sterling has the advantage that the activity of enzyme is higher than industrial enzyme, because of The operation do not lived through destructive enzymes such as extraction, dry, storages.

The endogenous enzymes of the single cell protein that the present invention provides is enriched, and can make again after self self-dissolving under suitable conditions For yeast, itself can be acted on yeast by nuclease after lysozyme lysis and protease.

Structure and the kind of endogenous enzymes are substantially distinguished from industrial enzyme, consideration based on cost, and existing yeast autolysis uses Exogenous enzyme be mostly protease, such as neutral protease, alkaline protease and papain etc., these are mainly used for enzymolysis Albumen in yeast is the least to the nucleic acid effect in yeast, so the nucleotide that the self-dissolving of existing yeast produces remains by ferment Female self-dissolving is that nuclease produces, and therefore, the yield of nucleotide is the highest.Industry protease as exogenous enzyme another lack Point is to produce bitter peptides, and this is the point of contact, position due to protease effect, the hydrophobicity when Proteolytic enzyme, in peptide chain Aminoacid fully comes out, and contact taste bud produces bitter in the mouth, and this also there will be when yeast autolysis, the simply delicate flavour contained by yeast Material is more to be masked this and manifests, but this can reduce the delicate flavour of yeast itself.Contrary uses the endogenous of single cell protein Enzyme acts on yeast would not produce the problems referred to above, the single cell protein in the present invention contain three kinds of enzyme lysozyme, nuclease and Protease, lysozyme can fully crack the cell wall of single cell protein and the cell wall of yeast, so that endogenous enzymes and the end Thing (yeast Dissolve things inside) can be fully contacted, simultaneously the nucleic acid of the nuclease of single cell protein and protease abundant enzymatic yeast again And albumen, thus producing the nucleotide of high-load and little peptide, the bitter peptides produced during protease hydrolyzed in tropina can show The industrial protease less than routine write.

The ratio of the single cell protein containing exogenous enzyme Yu yeast has been arrived optimal ratio model by optimum experimental by the present invention Enclose so that enzyme and substrate can fully react, such that it is able to produce than more preferable nucleotide and little in the case of both independent self-dissolvings Peptide content.

Yeast autolysate production technology has the advantage that

Directly using the remarkable advantage in production technology of the single cell protein enzymatic yeast containing endogenous enzymes is certainly Time molten requiring moisture looser and much lower than the enzyme of industry, existing yeast autolysis is using industrial enzyme such as neutral egg May require that the moisture of self-dissolving is the highest during white enzyme, and need that there is the highest mobility.Can cause in most cases The moisture of self-dissolving material needs higher than 95%, and based on this, existing yeast autolysis is used mostly enzymatic vessel and produces.This The single cell protein that invention uses can contain substantial amounts of ICW, along with cell rupture during self-dissolving, cell when non-self-dissolving Interior moisture can flow out extracellular, thus improves the mobility that self-dissolving is material, therefore, and a weight when using single cell protein Extra high moisture when to want advantage be to have no need to ensure that yeast autolysis in technique, during production, moisture only need to control 70%～95%, therefore, only the equipment that can fully be stirred self-dissolving need to be used.The moisture of self-dissolving reduces permissible Save substantial amounts of drying cost, thus reduce the cost of final products.

The temperature range of the self-dissolving technology that the present invention provides is higher than existing self-dissolving technology, and temperature controlled scope is high In 68 DEG C of pasteurization, can not worry that harmful microbe is bred, thus reduce the control difficulty of temperature.Because this In invention, the optimal reactive temperature of endogenous enzymes kind and existing industrial protease has bigger difference, the single cell protein of the present invention Optimum temperature to be significantly higher than common protease.

In the yeast autolysis parameter that the present invention provides, the self-dissolving time is significantly lower than existing all self-dissolving methods, and reason is also It is relevant with the enzymatic activity height of single cell protein.The shortening of self-dissolving time can also save substantial amounts of production cost.

The self-dissolving technology using the present invention can make the nucleotide content of yeast can obtain bigger lifting than prior art, The free nucleotide of product, little peptide, free amino acid to be significantly higher than existing yeast autolysate.

Due to self-dissolving cost, the significantly reducing of drying cost, the cost of product also significantly to drop with existing yeast product It is low, so that yeast product can have larger range of application.The yeast product made enters due to effective ingredient such as nucleotide One step improves so that the palatability of product, abnormal smells from the patient obtain bigger reinforcement.Nucleotide content raising also contributes to product and is improving Animal immune improves production performance aspect bigger effect.Therefore, compare with existing yeast autolysate, hydrolysate in application Preferably performance is just had when animal feed.Although the single cell protein in the present invention uses as exogenous enzyme, but owing to adding Dosage is high, final products contribute to the most again a part of nutritional labeling, also contributed to the protein ingredient of significant proportion, thus Also the yield of final products is improved.

Accompanying drawing explanation

Fig. 1 is that the present invention utilizes the food and drink production waste water unicellular compound enzymic preparation of cultivation to prepare yeast as exogenous enzyme The process flow diagram of autolysate.

Fig. 2 is the structural representation of self-dissolving equipment.

Fig. 3 is the process flow diagram that brewing wastewater produces microbial compound enzyme preparation.

Fig. 4 is the process flow diagram that amino acids production waste water produces microbial compound enzyme preparation.

Wherein, belt wheel 1. reductor 2. travelling gear 3. cover plate 4. charging aperture 5. low-temperature zone heating hollow blade 6. cavity steams Vapour air inlet 7. high temperature section heating hollow blade 8. temperature sensor 9. spray equipment 10. cavity steam exhaust-gas mouth 11. overflow plate 12. discharge gate 13. equipment and horizontal plane angle 14. high temperature section steam exhaust-gas mouth 15. high temperature section steam inlet 16. motor 17. main casing 18. transmission main shaft 19. low-temperature zone steam inlet 20. low-temperature zone steam exhaust-gas mouth 21. bearing 22..

Centrifuge 100, board and frame machine 101, grid maker 102, regulating reservoir 103, PH regulates tank 104, preliminary sedimentation tank 105, biological choosing Selecting pond 106, Aeration tank 107, second pond 108, holding vessel 109, centrifuge 110, containing diatomite waste water 120, containing yeast wastewater 121, other malting effluents 122, waste residue 123, concentrated acid 124, concentrated base 125, water outlet 126, dilution water 127, trace element 128, phosphorus 129, nitrogen 130, reflux bacterium mud 131, enzyme preparation 132

Fine grid machine 200, regulating reservoir 201, preliminary sedimentation tank 202, sump 203, SBR Aeration tank 204, holding vessel 205, sheet frame Machine 206, flash drying equipment 207, amino acids production waste water 210, concentrated base 211, trace element 212, phosphorus 213, nitrogen 214, dilution Water 215, bacterium mud 216, waste residue 217, water outlet 218

The invention will be further described below in conjunction with the accompanying drawings, and the principle of this method and the equipment of employing are to this specialty It is the most clearly for people.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to limit Determine the present invention.

Detailed description of the invention

The single cell protein compound enzymic preparation that the present invention utilizes food and drink to produce waste water cultivation prepares yeast autolysate Method, is to carry out mixing self-dissolving then in the ratio of dry biomass (2:8)～(8:2) with yeast with unicellular compound enzymic preparation Be dried, sterilizing, self-dissolving is divided into low-temperature zone and high temperature section, and low-temperature zone keeps 20～60 minutes at 45～60 DEG C, high temperature section 75～ 95 DEG C keep 30～120 minutes, and wherein, described unicellular compound enzymic preparation is that food and drink produces waste water by following steps institute Obtain:

(1) food and drink producing waste water and carry out pretreatment, slagging-off oil removing also adjusts pH to 5～9, and residue clear liquid is without mud The material that sand, bulky grain Organic substance etc. can precipitate, pretreated food and drink produces the BOD concentration of waste water at 100- Between 5000mg/L；

(2) pretreated food and drink is produced waste water and carry out nutrient substance regulation, make to supplement after nitrogen, phosphorus in waste water BOD: total nitrogen: total phosphorus=100:(5-20): (0.5-2)；

(3) using the usual aerobic activity flora of environmental protection industry (epi) as the production thalline of unicellular compound enzymic preparation, described multiple The thalline that produces of synthase preparation includes the one from hair zygosaccharomyces, Micrococcus, corynebacterium, bacillus, will The production thalline of compound enzymic preparation is inoculated into aeration culture pond, and the waste water after step (2) nutrient substance regulates pumps into aeration training Supporting pond, be sufficiently mixed with inoculation thalline, thalline is cultivated in the breeding of aeration culture pond, and omnidistance aeration oxygen replenishing ensures that aeration culture pond is molten Solution oxygen is 2～4mg/L, and temperature is limited to 10～40 DEG C, and pH is limited to 6.5～8.5, and thalline breeding obtains unicellular compound enzyme after cultivating Preparation, unicellular compound enzymic preparation precipitates with flock and microbial film form and exists with bacterium pureed state；

(4) bacterium mud is collected and is obtained unicellular compound enzymic preparation.

The food and drink waste water of the present invention includes starch or sugar or beverage or canned food or meat or Aquatic product or makes The production phase water of wine or rice product or milk product or bean product or fermented seasonings, described production phase water Including food and drink raw material soaking, germinate, pulverize, squeeze, extract, dilute, concentrate, during steaming and decocting is fried the cleaning that produces give up Water, or saccharifying, the filtered wastewater that ferments, remove the gred, separate, produce in purification process, or the finished product of evaporating, emitting, dripping or leaking of liquid or gas in packaging process And clean the waste water produced.

Wherein preferred brewing wastewater.Brewing wastewater is pressed content of organics and is divided, and can be divided three classes: cleaning waste water: fridge, The cooling water of wheat juice, fermentation etc. and the flushing water etc. of bottle washer, be the clean water that can recycle；Clean waste water: produce dress The rinse water put, rinsing yeast water, the bottle-washing water etc. of racking room of fermentation plant, containing variable amount of Organic substance and inorganic Thing；Slag inclusion waste water: the organic wastewater of racking room and inorganic matter waste water.The concrete feature of distillery wastewater has following: make The source of wine waste water has complexity and multiformity；The water yield of discharge is big, and organic concentration is high, and suspended solids content is high, water Matter changes greatly；PH, COD, BOD of waste water are relatively stable, and BOD/COD value is higher, good biodegradability；Brewing wastewater is without poisonous Harmful substance.

Wherein another preferably amino acids production waste water.The waste water of amino acids production mostlys come from following side Face: waste liquid remaining after fermentation liquid resin absorption.Mainly contain valine, isoleucine, leucine and part inorganic salt, belong to High concentrated organic wastewater；The waste liquid produced in ion exchange resin adsorption process, mainly contains organic pigment and acid, alkali etc. are inorganic Salt；Concentrate, filter the waste water produced；Cooling water.Amino acids production waste water specific features is as follows: organic concentration is higher, wherein The absorption waste liquid etc. that predominantly fermentation remaining stroma and ion exchange process are discharged, fermentation remaining stroma has Semen Maydis pulp, peanut cake Powder, starch, soybean cake powder, crack rice powder, peptone etc.；In waste water, float and colloidality solid concentration are high, wherein predominantly fermentation Residual media matter and fermentation produce the thread thalline of microorganism；Owing to aminoacid is that batch fermentation produces, waste water interval row Put, thus its waste water composition and the water yield great changes have taken place at any time.

Food and drink waste water should not contain source of heavy metal pollution, without poisonous harmful chemical agents polluter, without pathogenic bacterium The waste water containing large amount of organic of discharge in the food and drink course of processing of polluter.

Described slagging-off deoiling method is but is not limited only to neutralize, precipitation, air supporting, coagulation, clarification, and filtering, absorption, film divides From, at least one in ion exchange.

Nutritional labeling contained by food and drink waste water is frequently not growth of microorganism optimal proportion, the most supplementary nitrogen of warp, As the cultivation nutriment of aerobic activity flora after phosphorus and trace element；Described nitrogen, the magnitude of recruitment of phosphorus press BOD: total nitrogen: total phosphorus= 100:(5-20): the final proportioning of (0.5-2) carries out supplementing interpolation, described trace element is according to the requirement of target enzyme preparation and useless In water, this micronutrient levels carries out selecting and addition control.

The BOD concentration of food and drink waste water is the highest is unfavorable for that microorganism quickly absorbs, and when waste strength is higher, needs Below BOD to 5000mg/L to be diluted.

Described supplementary nitrogen source is but is not limited only to ammonia, ammonium nitrate, ammonium phosphate, carbamide, the organic nitrogen of aquaculture discharge At least one in the itrogenous organic substance that source, commercial production are discharged.Described supplementary phosphorus source be but be not limited only to potassium dihydrogen phosphate, At least one in potassium hydrogen phosphate, ammonium dihydrogen phosphate, diammonium phosphate, perphosphate, tripolyphosphate, phosphorus stone.

Described supplementary trace element selection is but is not limited only at least in calcium, magnesium, ferrum, zinc, copper, potassium, molybdenum, manganese Kind.

Utilize this thalline to secrete the feature of a large amount of extracellular polymerics, form flock and microbial film.These flocks and Microbial film can all precipitate through backwash or after staticly settling, and exists with bacterium pureed state.A part of bacterium mud is as multiple Synthase preparation first product is collected in that to have the holding vessel of aeration performance standby, and remainder is back to aeration culture pond as inoculation liquid Proceed breeding to cultivate.The bacterium mud amount discharged as compound enzymic preparation first product is and the ratio of aeration culture pond bacterium mud total amount And determine with at least one in the ratio of water inlet total carbon quality, it is a constant numerical value.According to total with culture pond bacterium mud The ratio of amount, is only limitted to the one in 1/8,1/9,1/10,1/11,1/12,1/13,1/14,1/15,1/16.According to total carbon The ratio of quality, is only limitted to the one in 0.3,0.35,0.4,0.45,0.5,0.55,0.6,0.65.

Described cultural method includes continuous culture method and batch-type culture method.Wherein continuous culture method is intake, bacterium solution refluxes, Breeding is cultivated, mixed liquor precipitation, residue bacterium solution are discharged and be carried out continuously, and wherein batch-type culture method water inlet, breeding are cultivated, mixed liquor Precipitation, draining, residue bacterium solution discharge each stage are carried out in order, remain bacterium solution from intaking to and discharge one cycle of calculation, Zhou Erfu Beginning is repeated.Described batch-type culture method includes various remodeling, and have changes into continuously by water inlet, and have changes into part aeration Continuously.Have changes into water outlet continuously, but as long as also maintain the feature of sequence batch processing periodic duty, just should belong to batch-type training Support the scope of technique.

Described thalline breeding training method includes the formula that is thoroughly mixed and biofilm adhesion type.The formula that is wherein thoroughly mixed be flora with The form of flock is at aeration culture pond suspension growth, and mainly by staticly settling acquisition bacterium solution, biofilm adhesion type is that flora is attached On the carrier that culture pond places in advance, carry out growth and breeding, generally pass through backwash and staticly settle acquisition bacterium solution.

It is collected in as the bacterium mud of single cell protein compound enzymic preparation that to have the holding vessel of aeration performance standby.

The described holding vessel with aeration performance collecting bacterium mud, its tool stirs or circulates function, eliminates bacterium mud Deposition is got off, and ensures the dissolved oxygen ＞ 2mg/L of bacterium mud, and bacterium mud acquisition time is less than 2d.After reaching Production requirement amount, stop Aeration, bacterium mud, through precipitation, concentrates and is processed into single cell protein compound enzymic preparation for next step production.

The described gravitational settling that is precipitated as, after precipitation, bacterium mud moisture content is reduced to less than 97%.

After precipitation, thalline concentrates, and uses macromolecule organic flocculating agent to carry out bacterium mud flocculation, so by centrifuge or Person's pressure filter, its moisture content is further decreased to less than 84%.

The product parameters of the unicellular enzyme preparation obtained contains main functional enzyme and includes lysozyme, nuclease and egg White enzyme, it is adaptable to animal protein feed (be but be not limited only at least one in fish flour, blood plasma), plant protein fodder (be but Be not limited only at least one in bean cake, Semen arachidis hypogaeae dregs), the enzyme of microprotein (be but be not limited only at least one in yeast) Hydrolysis and fermentation.

The parameter of single cell protein compound enzymic preparation single cell protein compound enzymic preparation is as follows:

Butt albumen 40.0%～60.0%, butt total amino acids 35.0%～55.0%, butt inorganic nitrogen≤5.0%, Butt total nucleotide 2.0%～6.0%, total nucleotide/total amino acids ratio >=0.07, total nucleotide/albumen >=0.05, bacteriolyze Enzyme 150-500U/g butt, nuclease 500-4000U/g butt, protease 1000-10000U/g butt,

Wherein, the single cell protein compound enzymic preparation single cell protein compound enzyme system that brewing wastewater produces preferably is utilized Agent:

Butt albumen 45.0%～55.0%, butt total amino acids 38.0%～50.0%, butt inorganic nitrogen≤3.0%, Butt total nucleotide 3.0%～5.0%, total nucleotide/total amino acids ratio >=0.08, total nucleotide/albumen >=0.06, bacteriolyze Enzyme activity 300～500U/g butt nuclease 2000～4000U/g butt, protease 5000～10000U/g butt.

The single cell protein of described unicellular compound enzymic preparation includes: Bacillus licheniformis, bacillus subtilis, not tally double Discrimination bacillus, enterococcus faecalis, enterococcus faecalis, lactoenterococcus, bacillus acidophilus, lactobacillus casei, Rhodopseudomonas palustris, length pair Discrimination bacillus, the combination of one or more microbial strain culture of bifidobacterium breve.

Yeast used in the present invention includes beer yeast, bakery yeast, molasses yeast.Wherein preferably beer yeast.Beer Brewer yeast can be the powder product being dried, it is also possible to be that the beer yeast without dehydration that brewery is expelled directly out concentrates Liquid can also be the yeast paste passing through filter-press dehydration.Described yeast, without nucleic acid extraction technique, wherein it is preferred that The beer yeast concentrated solution of undried and yeast paste.

The dry protein content of described beer yeast is 40%～55%.

The raw material composition of yeast autolysate of the present invention is made up of following raw material and dry biomass percentage ratio thereof: Single cell protein compound enzymic preparation 20～80%, yeast 20～80%, preferably single cell protein compound enzymic preparation 30～60%, Yeast 30～60%, more preferably single cell protein compound enzymic preparation 50%, yeast 50%.

Single cell protein and yeast are delivered to self-dissolving equipment in proportion carry out mixing self-dissolving process.Described self-dissolving equipment By heating up to twin screw hollow blade heat supply, described self-dissolving equipment uses conduction oil or steam to supply hollow blade Heat.Front the 1/3 to 1/2 of two twin screw hollow blades of described self-dissolving equipment is segmented into low-temperature zone.Purpose is to be heated by material Temperature needed for low temperature self-dissolving, low-temperature zone can also be used for the interpolation of exogenous enzyme, and remaining segment is high temperature self-dissolving section, it is therefore an objective to continue Continuous be heated to higher from solubility temperature；

The cavity of self-dissolving equipment has an angle of inclination, and the feed end of self-dissolving equipment is higher, can make the material can be more Fast flows to discharge end, and described cavity is 3 °～10 ° with the range of tilt angles of horizontal plane；

Self-dissolving equipment is provided with the shower nozzle of spray on chamber top, is fitted with spray in leading portion, stage casing and the rear end of equipment Head, the purposes one of shower nozzle is to spray exogenous enzyme or autolysis promoter in autolytic process；Two is spray sterilization after self-dissolving terminates Liquid is with cleaning and sterilizing equipment.

The temperature requirement of self-dissolving is: low-temperature zone temperature is maintained at 45～60 DEG C, and high temperature section temperature is maintained at 75～95 DEG C

The time requirement of self-dissolving is: material should be maintained at 20～60 minutes in the time of low-temperature zone, and material stops in high temperature section The time stayed should be maintained at 30～120 minutes.

Self-dissolving material moisture requirement: the moisture of mixed material should be not less than 75%.

Material after self-dissolving is delivered to drying equipment by screw conveyor and is dried.Described drying equipment is single rolling Drum drier or cyclonic airflow drying machine, dried material moisture is 12%～25%.

Material after drying is delivered to tunnel type micro wave sterilizing installation by conveying equipment and carries out sterilizing, sterilization time 5 ～15 minutes, after sterilizing, moisture content of material is 5%～15%.

Material after sterilized uses Ultra-Micro Grinding Equipment to pulverize.Described Ultra-Micro Grinding Equipment can be roll-type pulverizing Machine, it is also possible to be jet mill, smashing fineness 100% 60 mesh excessively, 95% crosses 80 mesh standard sieves.

Finally, packed products.

Thus obtained yeast autolysate product moisture content 5.0%-10.0%；Protein content 40.0%-60.0%； Free nucleotide content 1.40%-3.00%；Free base contents 0-0.20%, little peptide content is not less than 15.00%, free ammonia Base acid content 2.0%-8.0%.

Embodiment 1: utilize beer waste water to produce the unicellular compound enzymic preparation for albumen feedstuff enzymolysis

Raw material: in beer waste water, main component is: saccharide, alcohols, aminoacid, pectin, Flos lupuli (Flos Humuli Lupuli), vitamin, ubiquitinated Compound and the Organic substance of packing shop and a small amount of inorganic salts etc..BOD/COD is higher, average out to 0.55.And have a large amount of float, Such as wheat slag etc., the most often there are the alkaline cleaner put into during disinfecting cleaning, antibacterial.Beer waste water water quantity and quality relies on raw In the product cycle, water quantity and quality fluctuation is the biggest.Production period wastewater flow rate is huge, and COD is 2000～4000mg/L, and pH value is with micro-alkali to middle alkali Property is main.

Production technology:

Production technology uses brewery existing Waste Water Treatment, slender through technique upgrading and operation control realization The production of born of the same parents' compound enzymic preparation.

Pretreatment of raw material.

1. filter: include machine 100 centrifugal filtration by centrifugation of containing diatomite waste water, extrude through board and frame machine 101 containing yeast wastewater Filtering, wheat husk, wheat slag, packaging label is through grid maker 102 grid filtration.After filtration, Waste Water Centralized is in regulating reservoir 103, regulating reservoir 103 volumes are not less than 2 times of per day displacement.Have not enough time to the containing diatomite waste water of filtration, containing yeast wastewater, cleaning alkali Water, liquefied ammonia are revealed waste water etc. and are pumped into accident pool, pump in regulating reservoir the most on a small quantity；

2.pH regulates: the waste water of regulating reservoir pumps into pH regulator tank 104, and the average water stopping time of pH regulator tank is 20s, and There is immersible pump continuously stirred.By adding concentrated hydrochloric acid, regulation wastewater pH is to 7.PH detection is by online pH probe detection, concentrated hydrochloric acid Interpolation by dosing pump, converter and pH numerical value co-controlling；

3. precipitation: the waste water regulating pH is pipelined to radial-flow preliminary sedimentation tank 105, by gravitational settling, inorganic Salt, silt and the kieselguhr not filtered out, yeast, wheat slag, wheat husk and packaging label precipitate and separate came, from preliminary sedimentation tank 105 end Portion is discharged to concentration basin, and supernatant is from the overflow of preliminary sedimentation tank top layer, the concentration of suspension < 500mg/L of effluent.Preliminary sedimentation tank is average Water stopping time is 4h, available depth 6.0m.

The regulation of nutrition content.Beer waste water the content of nitrogen and phosphorous wretched insufficiency, with carbamide and potassium dihydrogen phosphate be respectively Nitrogen source and the supplementary source of phosphorus source.

1. it is respectively mounted COD, total nitrogen, total phosphorus online detection instrument, according to BOD in radial-flow preliminary sedimentation tank overflow outlet: total Nitrogen: total phosphorus=100:8:1, wherein BOD=COD*0.55, calculates the magnitude of recruitment of total nitrogen, total phosphorus by integrated data；

2. carbamide and potassium dihydrogen phosphate are each configured to the aqueous solution of fixed concentration, by dosing pump and Frequency Converter Control, add Being added in waste water, the ancillary equipment of carbamide and potassium dihydrogen phosphate is newly-built equipment.The supplementary data conduction calculated in step 1 is to becoming Frequently device, and then regulation dosing pump flow.Point of addition is waste water is from preliminary sedimentation tank to the pipeline in bioselection pond；

3. supplementing of trace element is mainly zinc sulfate and manganese sulfate, with 2mg/L Zn2+'s in waste water and 1mg/L Mn2+ Concentration necessary carries out supplementing interpolation, and the interpolation equipment of trace element is newly-built equipment.Zinc sulfate and manganese sulfate are all diluted to fix The aqueous solution of concentration is added, and adds and is controlled by dosing pump,；

4., according to online COD data, determine dilution ratio, dilution waste water COD concentration to 2000mg/L.Dilution water be two sink Pond goes out supernatant, dilution water point of addition be waste water from preliminary sedimentation tank to the pipeline in bioselection pond, dilution ratio controls logical Cross dosing pump and Frequency Converter Control.

Microorganism culturing.

The microbial culture method of beer waste water is identical with original method of wastewater treatment with thalline breeding training method, respectively It it is continuous culture method and be thoroughly mixed formula.

1. beer waste water from preliminary sedimentation tank 105 to the conveyance conduit of Aeration tank 107 through nitrogen, phosphorus, the supplementing of trace element, dilute The bioselection pond, front end 106 being positioned at Aeration tank 107 it is directly entered after releasing the dilution of water.Bioselection pond 106 is some First supplementing of one of them of Aeration tank, beer waste water and nutrient substance mix with thalline at this.Thalline can derive from and connect Kind, but in the case of production is stable, thalline is not required to additionally inoculate, it is also possible to come from the backflow bacterium mud of second pond.Connect Kind thalline can use the aerobic activity flora that environmental protection industry (epi) is usual, such as from the unicellular Pseudomonas of hair, Micrococcus, corynebacterium One in genus, bacillus.

2. the bacterium mud that sedimentation in secondary sedimentation tank is got off is carried by pipeline, is back to bioselection pond, in bioselection pond, with beer Wine waste water is sufficiently mixed.Second pond bacterium mud reflux line is equipped with densitometer, effusion meter and electromagnetic valve, by Frequency Converter Control electromagnetism Valve opening so that the constant mass of backflow bacterium mud.Backflow bacterium shale amount determines according to Aeration tank temperature, when Aeration tank temperature is 10 ～when 20 DEG C, backflow bacterium mud concentration is not less than 20000mg/L, and backflow bacterium mud amount is 5Kg/ ton waste water；When Aeration tank temperature is 20 ～when 30 DEG C, backflow bacterium mud concentration is not less than 15000mg/L, and backflow bacterium mud amount is 3.75Kg/ ton waste water；When Aeration tank temperature exists When 30～40 DEG C, backflow bacterium mud concentration is not less than 10000mg/L, and backflow bacterium mud amount is 2.5Kg/ ton waste water.

3. bacterium mud and beer waste water are after bioselection pond is sufficiently mixed, and enter subordinate's Aeration tank, and thalline is numerous at Aeration tank Growing in incubation, omnidistance aeration oxygen replenishing guarantee culture pond dissolved oxygen is between 2～4mg/L, and pH, between 7.5～8.5, aoxidizes Reduction potential is between+200～+600mV.Aeration tank is open culture environment, and temperature is affected bigger by temperature, cultivates temperature Degree is limited to 10～40 DEG C.

4. Aeration tank continuum micromeehanics, in beer waste water, Organic substance is absorbed totally by thalline, and thalline is with cotton-shaped form It is thoroughly mixed in Aeration tank.In Aeration tank end overflow, mixed liquor delivers into second pond 108 by pipeline, at second pond Relying on action of gravity, cotton-shaped bacterial sediment gets off, and forms bacterium mud.These bacterium mud are discharged bottom second pond, and a part is for single Cell compound enzymic preparation produces the holding vessel 109 being sent to have aeration performance, and remainder is standby as backflow bacterium mud 131. The average water stopping time of beer waste water is 3d, and the bacterium mud amount produced for unicellular compound enzymic preparation is determined according to Aeration tank temperature Fixed, when Aeration tank temperature is at 10～20 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation accounts for Aeration tank total bacterium mud amount 1/14；When Aeration tank temperature is at 20～30 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation accounts for the total bacterium of Aeration tank The 1/12 of mud amount；When Aeration tank temperature is at 30～40 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation accounts for Aeration tank The 1/10 of total bacterium mud amount.

Produce unicellular compound enzymic preparation.

1. bacterium mud is collected: the bacterium mud being used for producing unicellular compound enzymic preparation of second pond discharge is collected in tool aeration performance Holding vessel 109 in, holding vessel is cylindrical, and pyrometric cone is arranged at bottom, is used for discharging high-concentration bacterial mud.Holding vessel aeration ensures bacterium The dissolved oxygen ＞ 2mg/L of mud.Holding vessel bacterium mud acquisition time is less than 2d.

2. Operational preparation: stopping aeration and the stirring of holding vessel, after bacterium mud precipitation 1h, concentration reaches more than 30000mg/L, Moisture content is less than 97%.

3. concentrate: bacterium mud pyrometric cone bottom holding vessel is discharged, and is pipelined to centrifuge entrance, and another pipeline is defeated Send high polymer coagulant PAM to centrifuge entrance.Bacterium mud and PAM mix at centrifuge, and bacterium mud flocculates and then centrifugal de-further Water, its moisture content is reduced to 82%.

Enzymolysis uses: the bacterium mud being centrifuged out is cake shape, is directly used in the fermentation of microprotein.The microorganism of liquid After the beer yeast powder of compound enzyme system and brewery's discharge is mixed in proportion stirring, it is directly used in the medicated beer ferment of this brewery discharge Female fermentation enzymolysis.

4. utilize the unicellular compound enzymic preparation that beer waste water produces:

Butt albumen 45.0%～55.0%, butt total amino acids 38.0%～50.0%, butt inorganic nitrogen≤3.0%, Butt total nucleotide 3.0%～5.0%, total nucleotide/total amino acids ratio >=0.08, total nucleotide/albumen >=0.06, bacteriolyze Enzyme activity 300～500U/g butt, nuclease 2000～4000U/g butt, protease 5000～10000U/g butt

Embodiment 2. molasses glutamic acid produces waste water and produces the unicellular compound enzymic preparation for albumen feedstuff enzymolysis

Raw material: the waste water specific features that molasses glutamic acid produces is as follows: waste liquid outward appearance is sepia, and there is a small amount of gas on surface Bubble, acidity is relatively strong, and organic concentration is higher, and COD load is up to 30000～70000mg/L, BOD loading 20000～42000mg/ L, suspension content is high, is 12000～20000mg/L.Containing a large amount of metabolic byproducts from microorganisms organic acid in waste water, mainly there is breast Acid, succinic acid etc., in molasses glutamic acid wastewater, total amino acid content is up to more than 7%, in addition to remaining glutamic acid, other residual amino Acid mainly has aspartic acid, alanine, glycine, pays propylhomoserin, leucine, isoleucine, the acid of phenylpropyl alcohol oxygen, lysine, arginine Deng, waste water also has residual sugar, carbamide etc., causes waste water NH₃-N is up to 6000～8000mg/L.

Production technology:

Production technology uses the existing Waste Water Treatment of glutamic acid factory, through technique upgrading and operation control realization The production of unicellular compound enzymic preparation.

Pretreatment.

1. filtering: waste water is promoted to fine grid machine 200 by intake pump, fine grid machine is for removing larger particles in waste water Suspension, floating thing, grid pitch selects 3mm；

2.pH regulates: the waste water after filtration is delivered to regulating reservoir 201 through pipeline, adds NaOH, pH and adjust at regulating reservoir water inlet Saving between 6-8, by online pH probe detection and control NaOH addition, be provided with bucket bottom regulating reservoir, it is heavy periodically to extract Shallow lake thing；

3. precipitation: regulating reservoir waste water pumps into flat flow preliminary sedimentation tank 202, makes the particle suspensions in waste water enter by action of gravity One step sedimentation, is collected by the scraper plate bottom preliminary sedimentation tank 202 and removes, and supernatant is in preliminary sedimentation tank end overflow.Preliminary sedimentation tank averagely stops The water time is 4h, available depth 4.0m.

The regulation of nutrition content.It is sufficient that molasses glutamic acid produces waste water nitrogen source, only need to supplement phosphorus source and the most micro- Secondary element.

1. the water outlet of flat flow preliminary sedimentation tank is pipelined to sump 203, and sump contains diving for stirring Water pump, immersible pump works always and makes waste water quality uniform, and sump contains a detection liquidometer, can calculate wastewater volume；

2., after preliminary sedimentation tank batch-type water inlet end, take sump water sample detection COD, total phosphorus, calculate waste water by liquidometer The water yield, according to COD: total phosphorus=100::1.5, calculates the magnitude of recruitment of total phosphorus；

3. calcium superphosphate is each configured to the aqueous solution of fixed concentration, disposably adds in sump 203 by centrifugal pump, The stirring utilizing immersible pump makes waste water quality uniform；

4. supplementing of trace element is mainly zinc sulfate and molybdenum trisulfate, with 2mg/L Zn in waste water²⁺With 1mg/L Mo²⁺'s Concentration necessary carries out supplementing interpolation.Zinc sulfate and molybdenum trisulfate are all diluted to the aqueous solution of fixed concentration and are added, addition manner Disposably added in sump by centrifugal pump, utilize the stirring of immersible pump to make waste water quality uniform；

5., according to COD data, determine dilution ratio, dilution waste water COD concentration to 5000mg/L.Dilution water is SBR aeration Pond 204 goes out supernatant, dilution water point of addition be waste water from sump to the pipeline of SBR Aeration tank, dilution ratio control By centrifugal pump, Valve controlling, determine valve opening by effusion meter.

Microorganism culturing.

Molasses glutamic acid produces the SBR technique of the microbial culture method selection batch-type culture method of waste water, and thalline is bred Training method selects to be thoroughly mixed formula.

1. fill phase, SBR Aeration tank need to contain certain volume and the bacterium mud dope of concentration, and persistently expose in this stage Gas, aeration ensures that DO is at about 1mg/L.Bacterium mud volume and concentration determine according to temperature during aeration, when aeration temperature 10～ When 20 DEG C, bacterium mud volume is not less than the 1/4 of effective volume, and mass concentration is not less than 20000mg/L；When Aeration tank temperature 20～ When 30 DEG C, bacterium mud volume is not less than the 1/5 of effective volume, and mass concentration is not less than 20000mg/L；When Aeration tank temperature 30～ When 40 DEG C, bacterium mud volume is not less than the 1/5 of effective volume, and mass concentration is not less than 15000mg/L.Fill phase, flooding time For 0.5h, constant flow rate is intake.

2. aeration phase, amino acids production waste water and bacterium mud are thoroughly mixed, and thalline absorbs having in amino acid wastewater Machine material metabolism, thalline is thoroughly mixed in SBR Aeration tank with cotton-shaped form.This stage whole process aeration oxygen replenishing ensures SBR aeration tank dissolved oxygen between 1～2mg/L, pH between 7.5～8.5, oxidation-reduction potential+200～+400mV it Between.SBR Aeration tank is open culture environment, and temperature is affected bigger by temperature, and cultivation temperature is limited to 10～40 DEG C.Aeration rank Section, the persistent period is 6h.

3. precipitate phase, after stopping aeration, relies on action of gravity, and cotton-shaped bacterial sediment gets off, and forms bacterium mud.Aeration rank Section, the persistent period is 0.5h.

4. draining and spoil disposal stage, draining and spoil disposal stage are carried out simultaneously, to ensure bacterium shale amount.Water decanter is passed through in draining Discharge.Spoil disposal refers to that bacterium mud is discharged bottom SBR Aeration tank, produces for unicellular compound enzymic preparation, remainder bacterium mud conduct It is standby as inoculation liquid that inoculation bacterium mud stays SBR Aeration tank.The bacterium mud amount produced for unicellular compound enzymic preparation exposes according to SBR Gas pond temperature determines, when Aeration tank temperature is at 10～20 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation accounts for SBR exposure The 1/12 of gas Chi total bacterium mud amount；When Aeration tank temperature is at 20～30 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation Account for the 1/10 of SBR Aeration tank total bacterium mud amount；When Aeration tank temperature is at 30～40 DEG C, produce for unicellular compound enzymic preparation Bacterium mud amount accounts for the 1/8 of SBR Aeration tank total bacterium mud amount.

Produce unicellular compound enzymic preparation:

Molasses glutamic acid is utilized to produce the unicellular compound enzymic preparation batch-type discharge that waste water produces, after selecting kept dry Use.

1. bacterium mud is collected: the storage being collected in tool aeration performance for producing the bacterium mud of unicellular compound enzymic preparation of discharge In tank 205, holding vessel is cylindrical, and pyrometric cone is arranged at bottom, is used for discharging high-concentration bacterial mud.Holding vessel aeration ensures the molten of bacterium mud Solve oxygen ＞ 2mg/L.Holding vessel bacterium mud acquisition time is less than 2d.

2. Operational preparation: stop the aeration of holding vessel 205 and stirring, after bacterium mud precipitation 1h, concentration reach 30000mg/L with On, moisture content is less than 97%.

3. concentrate: bacterium mud pyrometric cone bottom holding vessel is discharged, and is pipelined to flocculation basin, and flocculation basin continues constant current Adding high polymer coagulant PAM, bacterium mud and PAM to mix at flocculation basin, overflow enters board and frame machine 206, and bacterium mud is dehydrated further, its Moisture content is reduced to 80%.

4. be dried: bacterium mud is transported to flash drying equipment 207 by auger, bacterium mud baking temperature 70 DEG C, drying time 2s, Moisture content is reduced to less than 20%.

5. enzymolysis uses: dried bacterium mud is powdery, may be used for the fermentation of the microprotein of good fluidity.Bacterium mud After being mixed in proportion stirring with wet yeast powder, participate in the enzymolysis of yeast as unicellular compound enzymic preparation.

Product parameters:

Utilize molasses glutamic acid produce waste water produce unicellular compound enzymic preparation:

Butt albumen 45.0%～65.0%

Butt total amino acids 38.0%～55.0%

Butt inorganic nitrogen≤3.0%

Butt total nucleotide 3.0%～5.0%

Total nucleotide/total amino acids ratio >=0.08

Total nucleotide/albumen >=0.06

Antalzyme activity 300～500U/g butt

Nuclease 2000～4000U/g butt

Protease 5000～10000U/g butt

Embodiment 3: this example is the manufacture example that raw material is chosen, self-dissolving produces

The composite flora rich in endogenous enzymes that the organic wastewater of this example use Brewery is cultivated in real time is as unicellular multiple The raw material of synthase preparation, composite flora goes out most cells free surface moisture by the operation of pelleting centrifugation after cultivating, bacterium after precipitation Group's moisture content is 90%～98%, and after centrifuge dehydration, moisture content is reduced to 80%～85%, obtained unicellular compound enzyme system The protein content (butt) of agent is higher than 50%, is delivered to self-dissolving equipment by measurable screw pump.

Yeast uses the non-autolysed brewer yeast of commercially available common drying, and protein content is 50%.

Ready unicellular compound enzymic preparation is added in dry 50%/50% ratio with yeast, ratio Interpolation is achieved in that and is realized by the electric machine frequency of screw-rod delivery pump and conveying flood dragon.

Self-dissolving mixture is in addition to composite flora has yeast, it is also possible to add carrier auxiliary material.

Material is delivered to self-dissolving equipment according to fixed proportion and carries out self-dissolving process.The self-dissolving equipment of the present embodiment is by double spiral shells Bar hollow blade equipment is provided.The temperature requirement of self-dissolving is: low-temperature zone temperature of charge is maintained at 55 DEG C, the time be 30 minutes high Temperature section temperature of charge is maintained at 70 DEG C, and the time is 60 minutes, and self-dissolving material moisture requirement is: mixed material moisture is not less than 75%, spray clear water by shower nozzle toward material when mixed material moisture does not reaches and requires.

Material after self-dissolving is delivered to drying equipment and is dried, and described drying equipment is single drum drier, after drying Material moisture is 12%～20%.

Material after drying is delivered to tunnel type micro wave sterilizing installation by gas lift equipment and carries out sterilizing, during sterilizing Between 5～15 minutes, after sterilizing, moisture content of material is 5%～15%.The total number of molds of the material after sterilizing, total number of bacteria meet " GB 13078-2001 " forage health standard.

Material after sterilizing carries out micronizing through the broken equipment of air blast ultramicro powder.Fineness of materials after pulverizing is: 100% Crossing 80 mesh, 90% crosses 100 mesh.Pulverize qualified material package finished product.Described Ultra-Micro Grinding Equipment can be roller flour mill, It can also be jet mill.

It is as shown in table 1 that the yeast autolysate selecting above-mentioned raw materials and the above-mentioned production technology of use to make has quality index:

Table 1

Embodiment 4 is the embodiment that yeast autolysis produces, and this example uses the richness that the organic wastewater of Brewery is cultivated in real time Composite flora containing endogenous enzymes is as unicellular compound enzymic preparation raw material.

Composite flora goes out most cells free surface moisture by the operation of pelleting centrifugation after cultivating, it is thus achieved that protein content is higher than The unicellular compound enzymic preparation of 50%, is delivered to self-dissolving equipment by measurable screw pump.

Yeast uses and comes from the dehydration fresh yeast that Brewery is obtained by filter pressing, and undried processes, and albumen is (dry Base) higher than 50%, ready unicellular compound enzymic preparation is added in dry 50%/50% ratio with yeast, ratio Interpolation be achieved in that by screw-rod delivery pump and conveying flood dragon electric machine frequency realize.

Material is delivered to self-dissolving equipment according to fixed proportion and carries out self-dissolving process.The temperature requirement of self-dissolving is: low-temperature zone Temperature of charge is maintained at 55 DEG C, and the time is 30 minutes, and high temperature section temperature of charge is maintained at 75 DEG C, and the time is 30～40 minutes.From Molten low-temperature zone uses shower nozzle to spray protease to material, and addition is the 1.5% of self-dissolving material (butt).Self-dissolving material moisture Requirement is: mixed material moisture is not less than 75%, passes through shower nozzle when mixed material moisture does not reaches and requires toward thing Material sprays clear water.

Material after self-dissolving is delivered to drying equipment and is dried, and drying equipment is the dry drying machine of cyclonic airflow, is dried Rear material moisture is 15%～25%.

Material after drying is delivered to tunnel type micro wave sterilizing installation by conveying equipment and carries out sterilizing, sterilization time 5 ～15 minutes, after sterilizing, moisture content of material is 5%～15%.The total number of molds of the material after sterilizing, total number of bacteria meet " GB 13078-2001 " forage health standard.

Material after sterilizing carries out micronizing through the broken equipment of air blast ultramicro powder.Fineness of materials after pulverizing is: 100% Crossing 80 mesh, 95% crosses 100 mesh.Pulverize qualified material package finished product.

The quality that the yeast autolysate selecting above-mentioned raw materials and the above-mentioned production technology of use to make has in table 2 below refers to Mark:

Table 2

Embodiment 5: this example is the experimental example of high temperature section self-dissolving optimum temperature value

The optimal reactive temperature scope of endogenous enzymes contained in the single cell protein of this patent indication is obtained by gradient experiment, The determination of parameter is finally to determine with the content of the free nucleotide in product after self-dissolving and free base, because endogenous enzymes Play the height end reaction of efficiency on free nucleotide and free base the two core index.Little peptide and free amino acid Also being the product of self-dissolving and enzymolysis, but be not appropriate for the evaluation index as self-dissolving, just can obtain because adding protease in a large number Obtain little peptide and the free amino acid of very high-load, but only free nucleotide just can be under suitable Autolysis Condition nuclease effect Produce.

Obtaining high temperature section from the experimental program of solubility temperature is most preferably:

Single cell protein in this example is the composite flora that beer waste water is cultivated, and dry protein content reaches 50%.Choosing Take just cultivation end composite flora to be positioned in beaker, for independent self-dissolving, check its Autolysis Condition.

Being positioned in thermostat water bath by beaker, use electric blender continuously stirred during self-dissolving, the moisture content of material is not Less than 80%.

Totally 180 minutes self-dissolving time, took out sample segment every 1 hour and carry out processing and for measuring.

The thermograde of self-dissolving see table, and material measures free nucleotide after drying through microwave sterilizating after self-dissolving and dissociates Base contents, its content such as table 3 below and table 4.

Table 3

From above-mentioned table, can be seen that the efficiency played when the endogenous enzymes of single cell protein is more than 65 DEG C starts to improve, The free nucleotide content obtained in the range of 70 DEG C～85 DEG C is the highest.

Table 4

Base from above-mentioned base data and chart it can be seen that in the case of less than 70 DEG C, in autolytic process Increase very fast, each experimental group data of 70 DEG C～95 DEG C it can be seen that the growth of base slowly, illustrates as Pasteur The key temperatures of sterilizing, when self-dissolving is higher than 70 DEG C, harmful microbe growth is suppressed significantly, and also would not self-dissolving be produced Free nucleotide be degraded to base.

Comprehensively the data of free nucleotide and base are it can be seen that temperature self-dissolving efficiency when 60 DEG C starts to step up, When 70 DEG C, self-dissolving efficiency reaches the highest, it can be seen that the temperature range self-dissolving efficiency of 60 DEG C～70 DEG C is the lowest, but main lacking Point be this temperature range be harmful microbe optimum growth temperature, thus cause substantial amounts of free nucleotide by harmful microorganism Degraded.

On the other hand, 85 DEG C～the 95 DEG C free nucleotide content still having a high level are it can be seen that endobacillary bacteriolyze Enzyme, nuclease optimal reactive temperature scope is wide and temperature is high, and is significantly higher than existing industrial enzyme enzyme.Microorganism molten Bacterium enzyme, nuclease are belonging to high temperature resistant enzyme, and optimum temperature is more than 60 DEG C and along with the liter enzymatic activity high of temperature is the most aobvious Write and reduce.

Therefore, according to the above-mentioned experimental result present invention by high temperature section from solubility temperature be selected in pasteurizing temperature (70 DEG C) with On, not only can play endogenous enzyme activity but also harmful microbe can be stoped to grow, thus it is higher to produce free nucleotide content Yeast product.

Embodiment 6: this example is the experimental example of high temperature section self-dissolving Best Times scope.

The high temperature section optimal reaction time parameter of the endogenous enzymes contained by the single cell protein of this patent indication is by gradient experiment Obtaining, the determination of parameter is also to be determined by the content of free nucleotide in product after self-dissolving and free base.

Obtaining the experimental program of high temperature section optimal self-dissolving time is:

The unicellular compound enzymic preparation that this example is used is the composite flora that Brewery organic wastewater is cultivated, unicellular multiple The mass content of synthase preparation accounts for the 50% of self-dissolving mixture, choose just cultivate the composite flora that terminates in beaker for reality Test.Being positioned in thermostat water bath by beaker, use electric blender continuously stirred during self-dissolving, material water ratio is not less than 80%.Choose embodiment 5 experiment acquisition high temperature section the suitableeest self-dissolving temperature range from solubility temperature and choose 70 DEG C respectively, 85 DEG C of two temperature Degree carries out the experiment of gradient self-dissolving time gradient.

The self-dissolving time, according to experiment gradient design, measured the free nucleotide after self-dissolving and free alkali in units of 1 hour Base content, see table.

Material needs through microwave sterilizating after self-dissolving, dried standby survey.

Table 5

Table 6

From above-mentioned data with table it can be seen that under 70 DEG C and two kinds of temperature conditionss of 85 temperature, free nucleotide content Rising the fastest in initial 2 hours of self-dissolving, persistently keep stable after 3 hours, nucleotide content no longer significantly improves.Explanation Endogenous enzymes is the lasting enzyme digestion reaction that carries out in initial 2 hours, and reaction is completely.In the case of 70 DEG C, free nucleotide In 3～4 hours, reach the highest, within the 5th hour, begin to decline, harmful microorganism continued propagation is described.In the case of 85 DEG C, 3 is little Shi Yihou free nucleotide persistently keeps stable with base contents, illustrates that enzyme digestion reaction substantially terminates and is harmful to micro-after 4 hours Biology is not bred.

Comprehensive Experiment result can be seen that the temperature more than 70 DEG C carries out self-dissolving, and the ideal time of self-dissolving is 1～4 little Time, the optimal time is 2～3 hours, and this self-dissolving time to illustrate un-extracted considerably less than existing yeast autolysis technique The endogenous enzymes enzymolysis efficiency of single cell protein to be significantly higher than the protease of industry.

Embodiment 7: this example is the test example of unicellular compound enzymic preparation and yeast optimal proportion.

Unicellular compound enzymic preparation is obtained as the optimal proportion of endogenous enzymes enzymatic yeast by experiment, because this patent The theoretical content of the endogenous enzymes contained by unicellular compound enzymic preparation mentioned and measured value cannot be carried out with yeast by calculating Accurately proportioning because both enzymolysis process to relate to process complex, so checking different mixed proportions by experiment The free nucleotide content that self-dissolving obtains can be determined that the suitableeest ratio.

The experimental program obtaining optimum mixture ratio example is:

Unicellular compound enzymic preparation in this example is the composite flora that Brewery organic wastewater is cultivated, it is also possible to be hay Bacillus cereus, dry protein content reaches 50%.

Described yeast is chosen Brewery and is obtained yeast, undried and biochemical extraction by filter pressing.

Ratio is to carry out proportioning with both dry matter contents.

The parameter of self-dissolving: temperature 70 C, 3 hours self-dissolving time, carries out self-dissolving in thermostat water bath, and material is constantly carried out Stirring.Material needs microwave sterilizating after self-dissolving, dried standby survey.

Table 7

It can be seen that yeast self-dissolving in the case of relying only on self can only produce and contain from above-mentioned experimental data and chart Measure the single cell protein rich in endogenous enzymes under relatively low free nucleotide, substantially less than equal in quality level.Along with at yeast The ratio of the unicellular compound enzymic preparation of middle interpolation steps up, and the free nucleotide that self-dissolving produces also steps up, and works as interpolation Amount when bringing up to 30% the content of the free nucleotide of mixture self-dissolving with use 100% single cell protein self-dissolving content phase With, continue the nucleotide output capacity that raising ratio is mixing self-dissolving and exceed both independent self-dissolvings.

Can analyze from result, endogenous enzymes contained in yeast and nucleic acid, relative endogenous enzymes content lacks, contrary The endogenous enzymes of the unicellular compound enzymic preparation that this patent is previously mentioned is enriched than nucleic acid content, therefore permissible when both mix self-dissolving Play the effect that enzymolysis substrate is complementary with enzyme preparation.Therefore, it can be noted that work as single cell protein contained in yeast from chart When reaching 60% in vain, the free nucleotide content after self-dissolving is the highest, and after illustrating to mix, the ratio of enzymolysis substrate and enzyme preparation is just Properly.

Embodiment 8: this example is feed intake test example.

The yeast autolysate being used this patent self-dissolving technique by the extra quality groove Preference test assessment of piglet is general with commercially available Logical yeast autolysate difference in terms of food calling and feed intake.The extra quality groove model test of piglet can measure piglet to specific former The preference of material local flavor, the feedstuff of its preference of searching for food for piglet can improve feed intake, and then improve the speed of growth.

Testing program:

Assessment raw material: the yeast autolysate in embodiment 2 contains albumen 54.6%, free nucleotide 2.33%, little peptide 29.4%, common commercially available yeast autolysate albumen is 51.3%, and free nucleotide is 1.65%, and little peptide content is 26.8.Test Feedstuff, basal diet is commercially available ablactational baby pig feedstuff, and test feed proportioning see table 11.

Experimental animal: select Weaning Age, body weight close, the ablactational baby pig searching for food normal, healthy 75, it is randomly divided into 5 Group.

Table 8

Test method:

The hopper 2 putting into material position in same hurdle identical with specifications and models (requires that the material position of each hopper must be with examination Test pig quantity consistent), in hopper, add a number of test feed respectively (require that the quantity of feedstuff must be sufficient, it is ensured that 2 The situation that feedstuff is inadequate is not had in hour), A hopper adds A material, and B hopper adds B material, allows pig free choice feeding collect material after 2 hours Remaining feedstuff measuring in groove, then adds B material in A hopper, adds A material and carry out above-mentioned identical test in B hopper.

During testing, observe two hoppers search for food the quantity of feedstuff pig, and with video camera and photographing unit track up two Hopper pig searches for food situation and pig number change situation.Require: test is carried out continuously 3～4 times, each 3～4 repetitions.Totally 3～5 My god, or a certain feedstuff search for food end till.

Result calculates:

Testing index: feed intake and partially addicted to index:

The A daily ration monophagia index=total feed intake of A group/total feed intake of B group

Table 9

Result of the test shows, under embodiment experimental condition, and the yeast that ablactational baby pig produces for this patent self-dissolving technology The feedstuff of autolysate preparation has Preference, and for higher containing its cost feedstuff feed intake, self-dissolving goes out the higher amount produced Free nucleotide and little peptide content show more preferable phagostimulating effect.

Claims (10)

1. utilizing food and drink to produce the method that the single cell protein compound enzymic preparation of waste water cultivation prepares yeast autolysate, it is special Levy and be: carry out mixing self-dissolving then in the ratio of dry biomass (2:8)～(8:2) with yeast with unicellular compound enzymic preparation Be dried, sterilizing, self-dissolving is divided into low-temperature zone and high temperature section, and low-temperature zone keeps 20～60 minutes at 45～60 DEG C, high temperature section 75～ 95 DEG C keep 30～120 minutes,

Described unicellular compound enzymic preparation is that food and drink production waste water is obtained by following steps:

(1) food and drink producing waste water and carry out pretreatment, slagging-off oil removing also adjusts pH to 5～9, and pretreated food and drink is raw Produce the BOD concentration of waste water between 100-5000mg/L, described food and drink produce waste water include starch or sugar or beverage, Or canned food or meat or Aquatic product or wine brewing or rice product or milk product or bean product or the production of fermented seasonings Stage water, described production phase use water include food and drink raw material soaking, germinate, pulverize, squeeze, extract, dilute, concentrate, The cleaning waste water that steaming and decocting produces during frying, or saccharifying, the filtered wastewater that ferments, remove the gred, separate, produce in purification process, Or in packaging process evaporating, emitting, dripping or leaking of liquid or gas finished product and clean produce waste water；

(2) pretreated food and drink is produced waste water and carries out nutrient substance regulation, make to supplement the BOD in waste water after nitrogen, phosphorus: Total nitrogen: total phosphorus=100:(5-20): (0.5-2)；

(3) using the usual aerobic activity flora of environmental protection industry (epi) as the production thalline of unicellular compound enzymic preparation, described compound enzyme The thalline that produces of preparation includes the one from hair zygosaccharomyces, Micrococcus, corynebacterium, bacillus, will be compound The production thalline of enzyme preparation is inoculated into aeration culture pond, and the waste water after step (2) nutrient substance regulates pumps into aeration culture pond, Being sufficiently mixed with inoculation thalline, thalline is cultivated in the breeding of aeration culture pond, and omnidistance aeration oxygen replenishing ensures aeration culture pond dissolved oxygen 2～4mg/L, temperature is limited to 10～40 DEG C, and pH is limited to 6.5～8.5, and thalline breeding obtains unicellular compound enzyme system after cultivating Agent, unicellular compound enzymic preparation precipitates with flock and microbial film form and exists with bacterium pureed state；

(4) bacterium mud is collected and is obtained unicellular compound enzymic preparation.

Method the most according to claim 1, it is characterised in that: when food and drink production waste water is brewing wastewater, slender The production equipment of born of the same parents' compound enzymic preparation include according to operation arrange filter plant, regulating reservoir, PH regulation tank, preliminary sedimentation tank, Aeration tank, Second pond, the holding vessel of tool aeration performance, production stage includes successively:

(1) brewing wastewater pretreatment: using centrifuge, flame filter press and grid maker as filter plant, the waste water of containing diatomite Machine is centrifuged by centrifugation, and the waste water containing yeast filters through board and frame machine, jointly filters through grid maker with other malting effluent, after filtration Waste Water Centralized, in regulating reservoir, is then pumped into pH regulator tank, regulates pH to 7；

(2) waste water regulating pH is pipelined to preliminary sedimentation tank, and the waste residue after precipitation is discharged bottom preliminary sedimentation tank, supernatant Flow out from preliminary sedimentation tank top layer overfall, be respectively mounted COD, total nitrogen, total phosphorus online detection instrument in preliminary sedimentation tank overflow outlet, according to BOD: total nitrogen: total phosphorus=100:8:1, wherein BOD=COD*0.55, calculates the magnitude of recruitment of total nitrogen, total phosphorus,

Using carbamide and potassium dihydrogen phosphate as nitrogen source and the supplementary source of phosphorus source, the aqueous solution being configured to fixed concentration adds to useless In water；

Using zinc sulfate and manganese sulfate as the supplementary interpolation of trace element, add concentration and press 2mg/L Zn in waste water²⁺And 1mg/L Mn²⁺Concentration necessary be added；

(3) microbial culture method of brewing wastewater selects continuous culture method, thalline breeding training method to select to be thoroughly mixed formula, Brewing wastewater is delivered to Aeration tank from preliminary sedimentation tank through pipeline,

Bacterium mud and brewing wastewater are sufficiently mixed at Aeration tank, and in Aeration tank breeding incubation, omnidistance aeration oxygen replenishing ensures to cultivate Pond dissolved oxygen is between 2～4mg/L, and pH is between 7.5～8.5, and Aeration tank is open culture environment, and thalline is with cotton-shaped shape Formula is blended in Aeration tank, and thalline mixed liquor delivers into second pond from Aeration tank end overflow, thalline mixed liquor by pipeline, Relying on action of gravity at second pond, cotton-shaped bacterial sediment gets off, and forms bacterium mud and discharges bottom second pond, being partly into tool The holding vessel of aeration performance is used for producing unicellular compound enzymic preparation, and another part is transmitted back to Aeration tank as backflow bacterium mud,

(4) the bacterium mud in holding vessel is processed into unicellular compound enzymic preparation product through precipitation, concentration.

Method the most according to claim 2, it is characterised in that: enter tool aeration performance holding vessel for unicellular multiple The bacterium mud amount that synthase preparation produces determines according to Aeration tank temperature, when Aeration tank temperature is at 10～20 DEG C, for unicellular multiple The bacterium mud amount that synthase preparation produces accounts for the 1/13～1/15 of Aeration tank total bacterium mud amount；When Aeration tank temperature is at 20～30 DEG C, use The bacterium mud amount produced in unicellular compound enzymic preparation accounts for the 1/11～1/13 of Aeration tank total bacterium mud amount；When Aeration tank temperature 30～ When 40 DEG C, the bacterium mud amount produced for unicellular compound enzymic preparation accounts for the 1/8～1/11 of Aeration tank total bacterium mud amount.

Method the most according to claim 1, it is characterised in that: when food and drink production waste water is amino acids production waste water Time, the production equipment of unicellular compound enzymic preparation include according to operation arrange filter plant, regulating reservoir, preliminary sedimentation tank, sump, SBR aeration culture pond, the holding vessel of tool aeration performance, production stage includes successively:

(1) amino acids production Wastewater Pretreatment: using grid maker as filter plant, waste water pump is delivered to grid maker and is filtered to remove useless Oarse-grained suspension, floating thing in water, grid pitch selects 3mm, and the waste water after filtration is delivered to regulating reservoir through pipeline, and pH regulator arrives Between 6-8, regulating reservoir waste water pumps into preliminary sedimentation tank, and precipitation waste residue is collected and removed, and supernatant is transported in sedimentation tank end overflow Sump；

(2) sump contain one for stirring immersible pump, take sump water sample detection TOC, total nitrogen, total phosphorus, according to TOC: Total nitrogen: total phosphorus=100:12:1.5, calculates the magnitude of recruitment of total nitrogen, total phosphorus, with ammonium nitrate and calcium superphosphate for nitrogen source and phosphorus source Supplementary source, disposably add in sump；

(3) microbial culture method of amino acids production waste water selects the SBR technique of batch-type culture method, thalline breeding cultivation side Formula selects to be thoroughly mixed formula, and the waste water in sump enters SBR aeration culture pond after the dilution of dilution water, trains at SBR aeration Support in pond and complete, into water, aeration, precipitation, draining, discharge of bacteria mud overall process, to continue aeration, aeration in fill phase according to time sequencing Ensure that DO is 0.8～1.2mg/L；Being thoroughly mixed at aeration phase amino acids production waste water and bacterium mud, thalline is with cotton-shaped form Be blended in SBR aeration culture pond, this stage whole process aeration oxygen replenishing ensure SBR aeration culture pond dissolved oxygen 1～2mg/L it Between, pH is between 7.5～8.5, and SBR aeration culture pond is open culture environment, and cultivation temperature is limited to 10～40 DEG C；Stop exposing After gas, cotton-shaped bacterial sediment gets off, and forms bacterium mud；Draining and spoil disposal stage are carried out simultaneously, and spoil disposal refers to that bacterium mud is cultivated from SBR aeration Discharge bottom pond, produce for unicellular compound enzymic preparation, stay part bacterium mud to stay SBR Aeration tank as connecing as inoculation bacterium mud Plant liquid standby；

(4) the bacterium mud being used for producing unicellular compound enzymic preparation discharged from SBR aeration culture pond is collected in tool aeration performance In holding vessel, holding vessel is at the dissolved oxygen of the aeration guarantee bacterium mud of collection phase > 2mg/L, stop holding vessel after having collected Aeration and stirring, bacterium mud prepares unicellular compound enzymic preparation through precipitation, concentration.

Method the most according to claim 4, it is characterised in that: it is combined for unicellular from what SBR aeration culture pond was discharged The bacterium mud amount that enzyme preparation produces determines according to SBR aeration culture pond temperature, when SBR aeration culture pond temperature is at 10～20 DEG C, The bacterium mud amount produced for unicellular compound enzymic preparation accounts for the 1/11～1/12 of SBR aeration culture pond total bacterium mud amount；When SBR aeration Culture pond temperature is when 20～30 DEG C, and the bacterium mud amount produced for unicellular compound enzymic preparation accounts for SBR aeration culture pond total bacterium mud The 1/9～1/10 of amount；When SBR aeration culture pond temperature

Method the most according to claim 1, it is characterised in that: the parameter of unicellular compound enzymic preparation is as follows:

Butt albumen 40.0%～60.0%；

Butt total amino acids 35.0%～55.0%；

Butt inorganic nitrogen 0～5.0%；

Butt total nucleotide 2.0%～6.0%；

Total nucleotide/total amino acids ratio 0.07～0.12；

Total nucleotide/albumen 0.05～0.10；

Antalzyme activity 150-500U/g butt；

Nuclease 500-4000U/g butt；

Protease 1000-10000U/g butt.

Method the most according to claim 1, it is characterised in that: unicellular compound enzymic preparation with the mixed proportion of yeast is (3:7)～(7:3), the most specific unicellular compound enzymic preparation is 1:1 with the mass content ratio of yeast.

Method the most according to claim 1, it is characterised in that: described yeast includes beer yeast, bakery yeast, molasses ferment One or more mixing in mother, and yeast is without the medicated beer ferment of nucleic acid extraction technique, preferably undried Female concentrated solution and yeast paste, the dry protein content of described beer yeast is 40%～55%.

9. by the yeast product obtained by the arbitrary described method of claim 1～8.

Yeast product the most according to claim 9, it is characterised in that: the biodiversity content in yeast product is 5.0%-10.0%, free nucleotide mass content are 1.40%-3.00%, free alkali base mass content is 0-0.20%, egg White mass content is 40.0%-60.0%, wherein the little peptide mass content in albumen be 15.00%～40.00%, in albumen Free amino acid mass content is 2.0%-8.0%.