CN106801073A - A kind of utilization corn syrup hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate - Google Patents
A kind of utilization corn syrup hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate Download PDFInfo
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Abstract
The present invention relates to the temperature sensitive type aminoglutaric acid fermentation production method that a kind of utilization corn syrup hydrolyzate substitutes part soybean meal hydrolysate, in fermentation process fermentation medium, fermenting and producing glutamic acid are prepared using corn syrup hydrolyzate instead of the part soybean meal hydrolysate in temperature sensitive type glutamic acid fermentation technique.The invention provides one kind with corn pulp as raw material, the method that part soybean meal hydrolysate prepares temperature sensitive type glutamic acid fermentation culture medium is substituted after hydrolysis, production target is basically identical with normal fermentation, accessory substance of the raw material from Starch Production, so with low cost, the method can effectively reduce the comprehensive production cost of glutamic acid while production stabilization is ensured, the economic benefit of enterprise is directly improved, is suitable to popularize in an all-round way aborning.
Description
Technical field
It is especially a kind of to substitute part dregs of beans using corn syrup hydrolyzate the invention belongs to bio-fermentation engineering field
The temperature sensitive type aminoglutaric acid fermentation production method of hydrolyzate.
Background technology
Temperature sensitive type glutamic acid fermentation raw material needs abundant organic nitrogen source, and breeding and be metabolized product acid for thalli growth provides institute
Required various amino acid and organic nitrogen, make thalli growth healthy and strong, and vigor enhancing largely accumulates glutamic acid.Soybean meal hydrolysate is
The organic nitrogen source commonly used in temperature sensitive type glutamic acid fermentation production technology, its price is of a relatively high, makes the cost of raw material higher.
Corn pulp is the accessory substance for producing cornstarch.In corn pulp containing abundant soluble protein, growth factor and
Some precursor substances, containing about 40%~50% dry.Corn pulp is the organic nitrogen source that microbial growth generally needs,
Therefore it is widely used in culture medium preparation in fermentation industry.Due in corn pulp Most amino-acids with the albumen shape of macromolecular
Formula is present, it is impossible to for microorganism directly utilizes, to meet the nutritional need of fermentation, it is necessary to add substantial amounts of corn pulp.With jade
Rice ＆ peanut milk consumption is increased, and more foam can be produced in fermentation process, and the effective rate of utilization that fermentation tank holds is decreased, in addition corn pulp
The microbial spores that middle insoluble granule and part are difficult high temperature killing increase therewith, fermentation microbiological contamination risk increase.
Temperature sensitive type glutamic acid fermentation technique nutritional need is big, and temperature sensitive type bacterial strain is sensitive to miscellaneous bacteria.If can be by corn
As fermentation raw material after pulp-water solution, the unfavorable factor in some productions can be eliminated, can make full use of the raw material of itself excellent again
Gesture, reduces cost increases the performance of enterprises.
By retrieval, following two patent publication us related to present patent application are found：
1. corn pulp enzymolysis liquid is used for the production method (CN103642857A) of microbe oil fermentation, the purpose of the present invention
Be provide corn pulp enzymolysis liquid replace corn pulp, for microbe oil fermentation production, to meet market the need for.Using plant
Sour enzyme and acid protease are centrifuged the enzymolysis liquid for obtaining and replace corn pulp as culture medium after being digested to corn pulp, effectively drop
Low microbiological contamination risk, Phos/total phosphorus content is 75-90% in enzymolysis liquid, and microorganism biological availability is improved, and reduction is produced into
This, and in microbe oil fermentation, be conducive to unrighted acid Rapid Accumulation, shorten fermentation period, reduce microorganism hair
Ferment cost.
2. a kind of preparation method of corn syrup hydrolyzate (CN103014085A), the present invention relates to field of amino acid fermentation,
Specifically related to a kind of preparation method of corn syrup hydrolyzate.The method takes the water of the corn pulp that Baume degrees is 12~25 ° and sulfuric acid
Solution mixing obtains the first solution, takes the hydrolysis of the first solution and obtains final product, and in terms of g/mL, sulfuric acid is with the mass volume ratio of the first solution
8~15:100.Corn syrup hydrolyzate prepared by the preparation method provided using the present invention is used for amino acid fermentation, and zymotic fluid is almost
There is no microbiological contamination, and using the corn pulp of conventional method preparation, there is different degrees of microbiological contamination situation, contamination rate in amino acid zymotic fluid
Up to 60%.Meanwhile, the corn pulp prepared using preparation method of the present invention is used for amino acid fermentation, and its amino acid produces sour water
It is flat to be significantly improved compared with the acid yield of the fermenting corn steep liquor prepared using conventional method.
By contrast, there is the difference of essence with above-mentioned patent publication us in present patent application.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art part, there is provided one kind is using corn syrup hydrolyzate replacement portion
Divide the temperature sensitive type aminoglutaric acid fermentation production method of soybean meal hydrolysate, the production method can stablize temperature sensitive type glutamic acid fermentation production water
It is flat, reduce the cost of raw material in production.
The purpose of the present invention is achieved through the following technical solutions：
A kind of utilization corn syrup hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate, fermentation
During replace the part soybean meal hydrolysate in temperature sensitive type glutamic acid fermentation technique to prepare fermented and cultured using corn syrup hydrolyzate
Base, fermenting and producing glutamic acid.
And, the corn syrup hydrolyzate prepares fermentation medium instead of 50% soybean meal hydrolysate.
And, the raw material proportioning of the fermentation medium is as follows：
40~60g/L of amylum hydrolysate of the sugar；20~30ml/L of corn pulp；20~40g/L of corn syrup hydrolyzate；Soybean meal hydrolysate
5~10g/L；5~15ml/L of molasses；7~9g/L of disodium hydrogen phosphate；2~4g/L of potassium chloride；1.4~1.6g/L of magnesium sulfate；Froth breaking
Agent 0.1~0.3ml/L, pH are 6.9~7.1,121 DEG C and sterilize 20 minutes.
And, the preparation method of the corn syrup hydrolyzate is as follows：Corn pulp uses the Acid hydrolysis, hydrolysising condition to be：Take
Fresh corn is starched, and adds 0.1~0.2 times of sulfuric acid of quality, and hydrolysis temperature is 100~120 DEG C, and hydrolysis time is 10~20 small
When, obtain final product the corn syrup hydrolyzate that hydrolyzate total nitrogen content is 3~4g/100mL, amino-acid nitrogen content is 1.5~3g/100mL.
And, the molasses are beet molasses.
And, the defoamer is polyoxyethylene polyoxy propyl alcohol amidogen ether.
And, comprise the following steps that：
In seed liquor access fermentation medium by 15% inoculum concentration by temperature sensitive type glutamic acid producer, using continuous stream plus nothing
The mode of machine nitrogen source is supplemented nitrogen source and controls the pH of zymotic fluid 6.8~7.2；Meanwhile, stream plus appropriate bubble enemy are extremely steeped as needed
Foam disappears, and stream plus the glucose solution that mass concentration is 500~800g/L control in mass fraction residual sugar to be 10g/L, fermentation
Culture terminated to after fermentation in 28~32 hours, that is, ferment to obtain glutamic acid；
Wherein, the glucose solution sterilizes 20 minutes through 115 DEG C.
And, it is described inorganic nitrogen-sourced for ammoniacal liquor or liquefied ammonia that mass fraction is 28%.
And, the mass concentration of the glucose solution is 600g/L.
The advantage for obtaining of the invention and good effect are：
1st, the invention provides one kind with corn pulp as raw material, replacement part soybean meal hydrolysate prepares temperature sensitive type after hydrolysis
The method of glutamic acid fermentation culture medium, production target is basically identical with normal fermentation, by-product of the raw material from Starch Production
Thing, so with low cost, the method can effectively reduce the comprehensive production cost of glutamic acid, directly while production stabilization is ensured
The economic benefit for improving enterprise is connect, is suitable to popularize in an all-round way aborning.
2nd, The method reduces the amount that corn pulp is directly added in fermentation medium, the foam in fermentation process reduces, no
Defoamer consumption is only saved, tank is also improved and is held effective rate of utilization.Simultaneously for subsequent extracted alleviates burden.
3rd, this method can kill miscellaneous bacteria therein and gemma in corn pulp hydrolytic process, can avoid because of raw material sterilizing not
Microbiological contamination risk caused by thoroughly.
4th, the whole simple operation of process of this method, remarkable in economical benefits, are very suitable for industrialized production.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention not limited to this,
Protection scope of the present invention can not be limited with following embodiments.
Experimental technique used in following embodiments is conventional method unless otherwise specified；The material that is used,
Reagent etc., unless otherwise specified, commercially obtains.
The present invention substitutes part soybean meal hydrolysate and prepares fermentation medium using corn syrup hydrolyzate during the fermentation, sends out
Ferment produces glutamic acid.Part soybean meal hydrolysate is substituted using corn syrup hydrolyzate of the present invention and prepares culture medium fermenting and producing
The operating method and process control condition of glutamic acid, if not illustrating, send out with the culture medium completely using soybean meal hydrolysate
The well-known operations method of ferment production temperature sensitive type glutamic acid strain fermenting and producing glutamic acid is consistent with process control condition.
Embodiment 1
A kind of utilization corn syrup hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate, fermentation
During replace the part soybean meal hydrolysate in temperature sensitive type glutamic acid fermentation technique to prepare fermented and cultured using corn syrup hydrolyzate
Base, fermenting and producing glutamic acid；Comprise the following steps that：
(1) microbial strains for being used are temperature sensitive type glutamic acid producer.
(2) fermentation medium：Amylum hydrolysate of the sugar 40g/L；Corn pulp 30ml/L；Corn syrup hydrolyzate 20g/L；
Soybean meal hydrolysate 5g/L；Molasses 5ml/L；Disodium hydrogen phosphate 7g/L；Potassium chloride 2g/L；Magnesium sulfate 1.4g/L；Froth breaking
Agent 0.1ml/L.PH is 6.9~7.1,121 DEG C and sterilizes 20 minutes.
(3) liquid glucose is added：It is the Glucose Liquid of 600g/L, 115 DEG C of sterilizings 20 to add the mass concentration that liquid glucose is glucose
Minute.
(4) seed liquor is accessed into fermentation medium by 15% inoculum concentration, using continuous stream plus the ammonia of mass fraction 28%
The mode of water is supplemented nitrogen source and controls the pH of zymotic fluid 6.8~7.2；Stream plus appropriate bubble enemy flow to lather collapse as needed
Plus residual sugar control in mass fraction to be 10g/L, fermented and cultured is to after 28 hours for the glucose solution of 600g/L for mass concentration
Fermentation ends, that is, ferment to obtain glutamic acid.
After testing, it is 176g/L that glutamate levels are produced in fermentation, and saccharic acid conversion ratio is 67%.
Embodiment 2
A kind of utilization corn syrup hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate, fermentation
During replace the part soybean meal hydrolysate in temperature sensitive type glutamic acid fermentation technique to prepare fermented and cultured using corn syrup hydrolyzate
Base, fermenting and producing glutamic acid；Comprise the following steps that：
(1) microbial strains for being used are temperature sensitive type glutamic acid producer.
(2) fermentation medium：Amylum hydrolysate of the sugar 50g/L；Corn pulp 30ml/L；Corn syrup hydrolyzate 30g/L；
Soybean meal hydrolysate 10g/L；Molasses 10ml/L；Disodium hydrogen phosphate 8g/L；Potassium chloride 3g/L；Magnesium sulfate 1.5g/L；Disappear
Infusion 0.2ml/L.PH is 6.9~7.1,121 DEG C and sterilizes 20 minutes；
The preparation method of the corn syrup hydrolyzate is as follows：Corn pulp uses the Acid hydrolysis, hydrolysising condition to be：Take fresh jade
Rice ＆ peanut milk, adds 0.1~0.2 times of sulfuric acid of quality, and hydrolysis temperature is 100~120 DEG C, and hydrolysis time is 10~20 hours, is obtained final product
The corn syrup hydrolyzate that hydrolyzate total nitrogen content is 3~4g/100mL, amino-acid nitrogen content is 1.5~3g/100mL.
The molasses are beet molasses.
The defoamer is polyoxyethylene polyoxy propyl alcohol amidogen ether.
(3) liquid glucose is added：It is the Glucose Liquid of 700g/L, 115 DEG C of sterilizings 20 to add the mass concentration that liquid glucose is glucose
Minute.
(4) seed liquor is accessed into fermentation medium by 15% inoculum concentration, using continuous stream plus the liquid of mass fraction 28%
The mode of ammonia is supplemented nitrogen source and controls the pH of zymotic fluid 6.8~7.2；Stream plus appropriate bubble enemy flow to lather collapse as needed
Plus residual sugar control in mass fraction to be 10g/L, fermented and cultured is to after 30 hours for the glucose solution of 700g/L for mass concentration
Fermentation ends, that is, ferment to obtain glutamic acid.
After testing, it is 183g/L that glutamate levels are produced in fermentation, and saccharic acid conversion ratio is 68%.
Embodiment 3
A kind of utilization corn syrup hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate, fermentation
During replace the part soybean meal hydrolysate in temperature sensitive type glutamic acid fermentation technique to prepare fermented and cultured using corn syrup hydrolyzate
Base, fermenting and producing glutamic acid；Comprise the following steps that：
(1) microbial strains for being used are temperature sensitive type glutamic acid producer.
(2) fermentation medium：Amylum hydrolysate of the sugar 60g/L；Corn pulp 30ml/L；Corn syrup hydrolyzate 40g/L；
Soybean meal hydrolysate 10g/L；Molasses 15ml/L；Disodium hydrogen phosphate 9g/L；Potassium chloride 4g/L；Magnesium sulfate 1.6g/L；Disappear
Infusion 0.3ml/L.PH is 6.9~7.1,121 DEG C and sterilizes 20 minutes；
The preparation method of the corn syrup hydrolyzate is as follows：Corn pulp uses the Acid hydrolysis, hydrolysising condition to be：Take fresh jade
Rice ＆ peanut milk, adds 0.1~0.2 times of sulfuric acid of quality, and hydrolysis temperature is 100~120 DEG C, and hydrolysis time is 10~20 hours, is obtained final product
The corn syrup hydrolyzate that hydrolyzate total nitrogen content is 3~4g/100mL, amino-acid nitrogen content is 1.5~3g/100mL.
The molasses are beet molasses.
The defoamer is polyoxyethylene polyoxy propyl alcohol amidogen ether.
(3) liquid glucose is added：It is the Glucose Liquid of 800g/L, 115 DEG C of sterilizings 20 to add the mass concentration that liquid glucose is glucose
Minute.
(4) seed liquor is accessed into fermentation medium by 15% inoculum concentration, using continuous stream plus the ammonia of mass fraction 28%
The mode of water is supplemented nitrogen source and controls the pH of zymotic fluid 6.8~7.2；Stream plus appropriate bubble enemy flow to lather collapse as needed
Plus residual sugar control in mass fraction to be 10g/L, fermented and cultured is to after 32 hours for the glucose solution of 800g/L for mass concentration
Fermentation ends, that is, ferment to obtain glutamic acid.
After testing, it is 190g/L that glutamate levels are produced in fermentation, and saccharic acid conversion ratio is 69%.
Related test results of the invention：
The culture medium fermenting and producing glutamic acid prepared using the method for the invention and the culture medium fermenting and producing known in
The technical indicator contrast of glutamic acid is as shown in table 1 below：
Culture medium fermenting and producing glutamic acid and the culture medium fermentation life known in that table 1 is prepared using the method for the invention
Produce the technical indicator contrast table of glutamic acid
Be can be seen that by above mill trial results and replace part soybean meal hydrolysate to carry out fermentation with corn syrup hydrolyzate matching somebody with somebody
Material, every production target of glutamic acid fermentation does not have obvious reduction, and acid production rate and saccharic acid conversion ratio are basically identical with former technique.
It is feasible to prove that the invention is popularized in an all-round way in temperature sensitive type glutamic acid fermentation technique productions.
Claims (9)
1. a kind of utilization corn syrup hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate, its feature
It is：The part soybean meal hydrolysate in temperature sensitive type glutamic acid fermentation technique is replaced to prepare using corn syrup hydrolyzate in fermentation process
Fermentation medium, fermenting and producing glutamic acid.
2. utilization corn syrup hydrolyzate according to claim 1 substitutes the temperature sensitive type glutamic acid fermentation of part soybean meal hydrolysate
Production method, it is characterised in that：The corn syrup hydrolyzate prepares fermentation medium instead of 50% soybean meal hydrolysate.
3. utilization corn syrup hydrolyzate according to claim 1 substitutes the temperature sensitive type glutamic acid fermentation of part soybean meal hydrolysate
Production method, it is characterised in that：The raw material proportioning of the fermentation medium is as follows：
40~60g/L of amylum hydrolysate of the sugar；20~30ml/L of corn pulp；20~40g/L of corn syrup hydrolyzate；Soybean meal hydrolysate 5~
10g/L；5~15ml/L of molasses；7~9g/L of disodium hydrogen phosphate；2~4g/L of potassium chloride；1.4~1.6g/L of magnesium sulfate；Defoamer
0.1~0.3ml/L, pH are 6.9~7.1,121 DEG C and sterilize 20 minutes.
4. utilization corn syrup hydrolyzate according to claim 3 substitutes the temperature sensitive type glutamic acid fermentation of part soybean meal hydrolysate
Production method, it is characterised in that：The preparation method of the corn syrup hydrolyzate is as follows：Corn pulp uses Acid hydrolysis, hydrolyzes bar
Part is：Fresh corn slurry is taken, 0.1~0.2 times of sulfuric acid of quality is added, hydrolysis temperature is 100~120 DEG C, and hydrolysis time is 10
~20 hours, obtain final product the corn pulp that hydrolyzate total nitrogen content is 3~4g/100mL, amino-acid nitrogen content is 1.5~3g/100mL
Hydrolyzate.
5. utilization corn syrup hydrolyzate according to claim 3 substitutes the temperature sensitive type glutamic acid fermentation of part soybean meal hydrolysate
Production method, it is characterised in that：The molasses are beet molasses.
6. utilization corn syrup hydrolyzate according to claim 3 substitutes the temperature sensitive type glutamic acid fermentation of part soybean meal hydrolysate
Production method, it is characterised in that：The defoamer is polyoxyethylene polyoxy propyl alcohol amidogen ether.
7. the utilization corn syrup hydrolyzate according to any one of claim 3 to 6 substitutes the temperature sensitive type of part soybean meal hydrolysate
Aminoglutaric acid fermentation production method, it is characterised in that：Comprise the following steps that：
In seed liquor access fermentation medium by 15% inoculum concentration by temperature sensitive type glutamic acid producer, using continuous stream plus inorganic nitrogen
The mode in source is supplemented nitrogen source and controls the pH of zymotic fluid 6.8~7.2；Meanwhile, stream plus appropriate bubble enemy to foam disappear as needed
Lose, stream plus the glucose solution that mass concentration is 500~800g/L control in mass fraction residual sugar to be 10g/L, fermented and cultured
Terminate to after fermentation in 28~32 hours, that is, ferment to obtain glutamic acid；
Wherein, the glucose solution sterilizes 20 minutes through 115 DEG C.
8. utilization corn syrup hydrolyzate according to claim 7 substitutes the temperature sensitive type glutamic acid fermentation of part soybean meal hydrolysate
Production method, it is characterised in that：It is described inorganic nitrogen-sourced for ammoniacal liquor or liquefied ammonia that mass fraction is 28%.
9. utilization corn syrup hydrolyzate according to claim 7 substitutes the temperature sensitive type glutamic acid fermentation of part soybean meal hydrolysate
Production method, it is characterised in that：The mass concentration of the glucose solution is 600g/L.
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| CN109943603A (en) * | 2019-03-11 | 2019-06-28 | 内蒙古阜丰生物科技有限公司 | A kind of amino acid production by ferment method method |
| CN110904163A (en) * | 2019-12-31 | 2020-03-24 | 内蒙古阜丰生物科技有限公司 | Method for improving lactic acid content of corn steep liquor |
| CN110923274A (en) * | 2019-12-18 | 2020-03-27 | 赵兰坤 | Method for preparing fermentation medium by using bean pulp hydrolysate |
| CN112662713A (en) * | 2020-12-28 | 2021-04-16 | 天津科技大学 | Culture medium for producing L-lysine by high-density fermentation and method thereof |
| CN113265432A (en) * | 2021-06-23 | 2021-08-17 | 内蒙古阜丰生物科技有限公司 | Fermentation medium and application thereof in preparation of amino acid by microbial fermentation |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109943603A (en) * | 2019-03-11 | 2019-06-28 | 内蒙古阜丰生物科技有限公司 | A kind of amino acid production by ferment method method |
| CN110923274A (en) * | 2019-12-18 | 2020-03-27 | 赵兰坤 | Method for preparing fermentation medium by using bean pulp hydrolysate |
| CN110904163A (en) * | 2019-12-31 | 2020-03-24 | 内蒙古阜丰生物科技有限公司 | Method for improving lactic acid content of corn steep liquor |
| CN112662713A (en) * | 2020-12-28 | 2021-04-16 | 天津科技大学 | Culture medium for producing L-lysine by high-density fermentation and method thereof |
| CN112662713B (en) * | 2020-12-28 | 2022-08-19 | 天津科技大学 | Culture medium for producing L-lysine by high-density fermentation and method thereof |
| CN113265432A (en) * | 2021-06-23 | 2021-08-17 | 内蒙古阜丰生物科技有限公司 | Fermentation medium and application thereof in preparation of amino acid by microbial fermentation |
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Application publication date: 20170606 |