CN106110276A - A kind of have the complex improving functional gastrointestinal disorder effect - Google Patents
A kind of have the complex improving functional gastrointestinal disorder effect Download PDFInfo
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- CN106110276A CN106110276A CN201610710536.1A CN201610710536A CN106110276A CN 106110276 A CN106110276 A CN 106110276A CN 201610710536 A CN201610710536 A CN 201610710536A CN 106110276 A CN106110276 A CN 106110276A
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- China
- Prior art keywords
- attapulgite
- rhizoma zingiberis
- zingiberis recens
- quintessence oil
- complex
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- 230000000694 effects Effects 0.000 title claims abstract description 19
- 208000014540 Functional gastrointestinal disease Diseases 0.000 title claims abstract description 10
- 208000018522 Gastrointestinal disease Diseases 0.000 title claims abstract description 10
- 229960000892 attapulgite Drugs 0.000 claims abstract description 106
- 229910052625 palygorskite Inorganic materials 0.000 claims abstract description 106
- 239000000843 powder Substances 0.000 claims abstract description 39
- NLDDIKRKFXEWBK-AWEZNQCLSA-N gingerol Chemical compound CCCCC[C@H](O)CC(=O)CCC1=CC=C(O)C(OC)=C1 NLDDIKRKFXEWBK-AWEZNQCLSA-N 0.000 claims abstract description 18
- JZLXEKNVCWMYHI-UHFFFAOYSA-N gingerol Natural products CCCCC(O)CC(=O)CCC1=CC=C(O)C(OC)=C1 JZLXEKNVCWMYHI-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000004927 clay Substances 0.000 claims abstract description 12
- 239000002775 capsule Substances 0.000 claims abstract description 7
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims abstract description 6
- 235000002780 gingerol Nutrition 0.000 claims abstract description 3
- 239000000546 pharmaceutical excipient Substances 0.000 claims abstract description 3
- 239000003921 oil Substances 0.000 abstract description 70
- 238000006243 chemical reaction Methods 0.000 abstract description 10
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 8
- 230000000845 anti-microbial effect Effects 0.000 abstract description 5
- 239000002260 anti-inflammatory agent Substances 0.000 abstract description 4
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- 238000010521 absorption reaction Methods 0.000 description 4
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
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- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 1
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- 125000000524 functional group Chemical group 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
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- 239000001963 growth medium Substances 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
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- 239000002609 medium Substances 0.000 description 1
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- 229910052901 montmorillonite Inorganic materials 0.000 description 1
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- 230000001681 protective effect Effects 0.000 description 1
- KKOXKGNSUHTUBV-UHFFFAOYSA-N racemic zingiberene Natural products CC(C)=CCCC(C)C1CC=C(C)C=C1 KKOXKGNSUHTUBV-UHFFFAOYSA-N 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
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- OJYLAHXKWMRDGS-UHFFFAOYSA-N zingerone Chemical compound COC1=CC(CCC(C)=O)=CC=C1O OJYLAHXKWMRDGS-UHFFFAOYSA-N 0.000 description 1
- KKOXKGNSUHTUBV-LSDHHAIUSA-N zingiberene Chemical compound CC(C)=CCC[C@H](C)[C@H]1CC=C(C)C=C1 KKOXKGNSUHTUBV-LSDHHAIUSA-N 0.000 description 1
- 229930001895 zingiberene Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9068—Zingiber, e.g. garden ginger
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Inorganic Chemistry (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a kind of complex having and improving functional gastrointestinal disorder effect, attapulgite powder generate through chemical reaction with Rhizoma Zingiberis Recens quintessence oil, both mass ratioes are 10:0.1 2；Described attapulgite powder, selects the attapulgite clay Ore type that attapulgite crystalline content is more than 50%, carbonate content is less than 10%, attapulgite clay is ground to 100 320 mesh；Described Rhizoma Zingiberis Recens quintessence oil is extracted by Rhizoma Zingiberis Recens, and wherein gingerol content is more than 20%.The new complex of the chemically composited formation of both attapulgite Rhizoma Zingiberis Recens quintessence oils has the collaborative advantage strengthening drug effect, can be mixed and made into powder or capsule with excipient.Attapulgite Rhizoma Zingiberis Recens quintessence oil complex of the present invention has protection gastrointestinal tract mucosa, recuperating gastrointestinal tract function, the effect of antimicrobial antiphlogistic；Evident in efficacy, use safety, effect to be better than on market conventional pharmaceutical products, have good potential applicability in clinical practice.
Description
Technical field
The invention belongs to functional food and biomedicine field, be particularly used for improving diarrhoea and cause functional gastrointestinal disorder
Oral formulations.
Background technology
Gastrointestinal function the most normally directly affects digesting and assimilating of food Middle nutrition composition, to maintaining each organ of human body
Normal function is most important.Unclean diet, bad dietary habit, tobacco and wine and medicine etc., all may result in diarrhoea, gastrointestinal function
Disorder, brings misery to patient, damages health.The medicine of preventing and treating diarrhoea at present mostly is diarrhea, antibacterials, there is treatment
Imitate that the best, side effect is big, be easily generated the problems such as drug resistance.In order to avoid the generation of this type of phenomenon, market demand is developed
Come from natural, effect preventing and treating diarrhoea and improving the new oral formulation of gastrointestinal function, to subtract significantly, without obvious adverse reaction
The misery of light patient.It is numerous that long-term diarrhea causes functional gastrointestinal disorder patient, therefore develops this type of formulation products and has good
Application prospect, is highly beneficial and necessary.
Attapulgite is the clay mineral of a kind of crystalloid aqueous zeopan of layer chain structure, due to the microcosmic that itself is unique
Structure, appearance and charge characteristics, attapulgite clay rod has many premium properties, such as colloidality, adsorptivity, antibiotic property and caking property
Deng, thus it is widely used in the fields such as chemical industry, medicine, agricultural and environmental protection.Attapulgite powder has absorption, removes multiple causing a disease
The ability of the factor.When attapulgite powder enters after gastrointestinal tract, can cover at gastrointestinal mucosal surfaces, thus repair and stablize gastrointestinal viscous
Envelope barrier, has restoration and protection effect to digestive tract mucosal barrier；Because of attapulgite can adsorb antibacterial in digestive tract, virus and
The toxin produced, thus reach to suppress bacterial multiplication, stop spreading, the effect of suppression inflammatory reaction.Concavo-convex in the U.S.
Rod is used for the treatment of diarrhoea with the form of oral OTC.Attapulgite, can be as inorganic matter or organic because of the particularity of its structure
Carrier, attapulgite is as the research of carrier, and great majority are at attapulgite intercalation inorganic matter, as the research of organic carrier
Seldom.
Rhizoma Zingiberis Recens is a kind of conventional flavouring agent, is also the health plant resource of traditional integration of edible and medicinal herbs, have cold relieving damp eliminating,
The multiple effects such as warming the stomach stomach invigorating, relaxing muscles and tendons to promote blood circulation, antiinflammatory.Ginger essential oil contains the multiple material with nutrition health-care functions, such as Rhizoma Zingiberis Recens
Alcohol, zingiberone, zingiberene, gingerol compounds, Rhizoma Zingiberis Recens quintessence oil has quite varied in fields such as food, cosmetics, health product, medicines
Application.Rhizoma Zingiberis Recens quintessence oil refers to the volatile oil obtained from race stem, in amber transparent oily liquids, has antimicrobial antiphlogistic, guarantor
The pharmacological actions such as liver function of gallbladder promoting.Active component the abundantest in Rhizoma Zingiberis Recens quintessence oil is 6-gingerol, exist thermo-labile, not acid and alkali-resistance, easily wave
Send out the feature that equistability is the best.
Summary of the invention
It is an object of the invention to provide a kind of complex having and improving functional gastrointestinal disorder effect, for attapulgite powder
The attapulgite Rhizoma Zingiberis Recens quintessence oil complex generated through chemical reaction with Rhizoma Zingiberis Recens quintessence oil, both can play the absorption of attapulgite, repair function, will cause
Sick microorganism is concentrated, the beneficially performance of Rhizoma Zingiberis Recens quintessence oil antibacterial action, raising anti-microbial property, can improve again the stability of Rhizoma Zingiberis Recens quintessence oil.
Described attapulgite powder is 10:0.1-2 with the mass ratio of Rhizoma Zingiberis Recens quintessence oil.
Described attapulgite powder, selects the attapulgite that attapulgite crystalline content is more than 50%, carbonate content is less than 10% to glue
Soil ore type, is ground to 100-320 mesh by attapulgite clay Ore；Described Rhizoma Zingiberis Recens quintessence oil is extracted by Rhizoma Zingiberis Recens, and wherein gingerol contains
Amount is more than 20%.
The invention also discloses the preparation technology of described attapulgite Rhizoma Zingiberis Recens quintessence oil complex:
(1) activation of attapulgite: a, take attapulgite powder add containing 20% hydrochloric acid beaker in, with plastic sheeting seal, 60 DEG C
Water-bath magnetic agitation 10h, reaction solution color from pale yellow color gradually becomes buff；
B, 10000rpm are centrifugal, remove supernatant, and deionized water wash precipitates repeatedly, saturating to supernatant pH value to neutrality, color
Bright；
C, by above-mentioned repeatedly washing after precipitation add in 10% sodium chloride solution, be ultrasonically formed suspension system, then 60 DEG C of water-bath magnetic
Power stirring 10h；
D, 10000rpm are centrifugal, take upper strata light gray solid, and in electric heating constant-temperature blowing drying box, 70 DEG C are dried to constant weight, after grinding
Cross 100-320 mesh sieve.
(2) preparation of Rhizoma Zingiberis Recens quintessence oil: take Rhizoma Zingiberis Recens, section, crushed after being dried are cleaned in peeling.Rhizoma Zingiberis Recens powder adds 70% alcohol reflux and carries
Take 2 times, add 10 times amount for the first time, extract 90min；Add 8 times amount for 2nd time, extract 60min, merge ethanol extract, filter, filter
Liquid concentrating under reduced pressure steams ethanol to the greatest extent, obtains Rhizoma Zingiberis Recens quintessence oil, uses HPLC method to measure 6-gingerol content.
(3) preparation of attapulgite Rhizoma Zingiberis Recens quintessence oil complex: first, takes the attapulgite powder after activation and adds in deionized water,
Ultrasonic disperse forms suspension system；Take Rhizoma Zingiberis Recens quintessence oil to be dissolved in dehydrated alcohol, be then added in attapulgite powder suspension system,
At temperature 30-90 DEG C, 2-3h is stirred on magnetic stirring apparatus；Then, it is precipitated after 10000rpm centrifugation, and collects
Clear liquid；Precipitate to be centrifuged again after 50% ethanol solution washing, again collect supernatant；Finally, the precipitation after above-mentioned washing is placed in
Electric heating constant-temperature blowing drying box is dried at 60 DEG C, ground 200 mesh sieves, the particle diameter 50-100 μm of resulting composite.
Described attapulgite Rhizoma Zingiberis Recens quintessence oil complex can be mixed and made into powder or capsule with excipient.
HPLC method is surveyed and is collected 6-gingerol content in supernatant, calculating 6-gingerol and the combination rate of attapulgite: E=(C0V0-
C1V1)/M×100%.Wherein E(%) it is the combination rate of 6-gingerol and attapulgite；C0And C1(mg/mL) it is respectively 6-gingerol
Initial concentration and the concentration collected in supernatant；V0And V1(mL) it is respectively initial soln and collects supernatant volume；M
(mg) for the attapulgite powder quality of activation.
Use single factor test and response surface experiments method, the reaction condition in preparation process is optimized.Investigate concavo-convex
Rod powder and the response time of Rhizoma Zingiberis Recens quintessence oil, 6-gingerol initial concentration, three kinds of factors of reaction temperature, determine the reaction condition of optimum.
The response time investigated is respectively 30 min, 60 min, 90 min, 120 min, 150 min, 180min；6-gingerol is the denseest
Degree is 60 mg mL-1、120 mg·mL-1、180 mg·mL-1、240 mg·mL-1、300 mg·mL-1；Reaction temperature is respectively
It is 30 DEG C, 45 DEG C, 60 DEG C, 75 DEG C, 90 DEG C.Result of the test shows, response time 120 min-180min, 6-gingerol initial concentration
180-240 mg·mL-1, reaction temperature be respectively 75 DEG C-90 DEG C for optimum reaction condition.Gained attapulgite Rhizoma Zingiberis Recens quintessence oil complex
For pale yellow powder solid, wherein 6-gingerol content is about 1%.
Gained attapulgite Rhizoma Zingiberis Recens quintessence oil complex is carried out a series of characterization research, and result of the test is as follows:
Infrared spectrum (FTIR) is analyzed: Fig. 1 is shown in by FTIR collection of illustrative plates.Attapulgite Rhizoma Zingiberis Recens quintessence oil complex has attapulgite at 1089cm-1,
3413cm-1, 3548cm-1And 3613cm-1The characteristic absorption peak at place, it may have Rhizoma Zingiberis Recens quintessence oil is at 1517cm-1, 2927cm-1With
2856cm-1The characteristic absorption peak at place；Show that Rhizoma Zingiberis Recens quintessence oil is already integrated in attapulgite structure.
X-ray photoelectron power spectrum (XPS) is analyzed: XPS scanning optical spectrum is shown in Fig. 2, Fig. 3.The signal of Si2p, C1s, O1s, Mg1s
Respectively appear in 102eV, 284eV, 532eV, 1303eV.Compared with attapulgite x-ray photoelectron power spectrum, attapulgite Rhizoma Zingiberis Recens quintessence oil is multiple
The elements Si on compound surface, O and Mg content have dropped 4.78%, 18.28% and 0.45% respectively, it may be possible to owing to Rhizoma Zingiberis Recens quintessence oil is recessed
The covering on convex rod surface.Rhizoma Zingiberis Recens quintessence oil main active 6-gingerol, molecular formula C17H2604, carbon element content is big；Attapulgite Rhizoma Zingiberis Recens essence
Oil composite surface carbon element content adds 24%, illustrates that attapulgite surface introduces the functional group of carbon atoms, XPS analysis
Prove Rhizoma Zingiberis Recens quintessence oil and the combination of attapulgite.
Thermogravimetric (TG) is analyzed: thermogravimetric curve is shown in Fig. 4, and attapulgite, attapulgite Rhizoma Zingiberis Recens quintessence oil complex are in different temperatures scope
Interior mass loss ratio is shown in Table 1.Thermogravimetric curve display attapulgite, attapulgite loaded Rhizoma Zingiberis Recens quintessence oil complex have four different
Dehydration: respectively owing to removing surface adsorption water and zeolite water (30 DEG C-100 DEG C), remaining zeolite water and part-structure
Water (100 DEG C-300 DEG C), the constitution water (300 DEG C-700 DEG C) of residual, the dehydroxylation (700 DEG C-800 DEG C) of Mg-OH group.?
100 DEG C-700 DEG C, attapulgite mass loss rate is significantly lower than attapulgite Rhizoma Zingiberis Recens quintessence oil complex, and the oxidation mainly due to Rhizoma Zingiberis Recens quintessence oil divides
Solve.According to the difference of weight-loss ratio, can estimate that in attapulgite Rhizoma Zingiberis Recens quintessence oil complex, the content of Rhizoma Zingiberis Recens quintessence oil is about 18.66%.Pass through thermogravimetric
Analyze, demonstrate again that the combination of Rhizoma Zingiberis Recens quintessence oil and attapulgite.
Table 1 attapulgite, attapulgite Rhizoma Zingiberis Recens quintessence oil complex mass loss ratio (%) in the range of different temperatures
Field emission scanning electron microscope (SEM) is analyzed: Fig. 5 is shown in by SEM collection of illustrative plates.Attapulgite, attapulgite Rhizoma Zingiberis Recens quintessence oil complex are by rod
Brilliant aggregate parallel closely forms brilliant bundle, and the brilliant interfascicular of rod the most mutually assembles formation aggregation, and in figure, (a) represents attapulgite, (b) table
Give instructions in reply compound.Fig. 5-1a, 1b(× 1000) visible, attapulgite Rhizoma Zingiberis Recens quintessence oil complex outward appearance Regularization degree significantly improves, particle diameter
Significantly increasing, profile becomes round cunning, and burr significantly reduces.Fig. 5-2a, 2b(× 20000) visible, attapulgite Rhizoma Zingiberis Recens quintessence oil complex group
Being polymerized to irregular form, edge blurry, specific surface area reduces, it may be possible to owing to the load of Rhizoma Zingiberis Recens quintessence oil makes the repulsion of complex
Power reduces.Fig. 5-3a, 3b(× 100000) visible, attapulgite Rhizoma Zingiberis Recens quintessence oil complex definition substantially reduces, surface image mould
Sticking with paste, electric conductivity is deteriorated, and shows organic load.Therefore, by the observation analysis of field emission scanning electron microscope, attapulgite
Load Rhizoma Zingiberis Recens quintessence oil, and morphologically there occurs change.
The present invention is compounded to form new complex by attapulgite and Rhizoma Zingiberis Recens Essential Oil Chemistry and has the collaborative advantage strengthening drug effect,
Preferably improve respective function, overcome respective weak point；And there is protection gastrointestinal tract mucosa, recuperating gastrointestinal tract merit
Energy, the effect of antimicrobial antiphlogistic；Evident in efficacy, use safety, effect to be better than on market conventional pharmaceutical products, have good facing
Bed application prospect.
Accompanying drawing explanation
Fig. 1 is attapulgite of the present invention, Rhizoma Zingiberis Recens quintessence oil and the infrared spectrogram of complex thereof；
Fig. 2 is the x-ray photoelectron energy spectrogram of attapulgite of the present invention；
Fig. 3 is the x-ray photoelectron energy spectrogram of attapulgite Rhizoma Zingiberis Recens quintessence oil complex of the present invention；
Fig. 4 is the thermogravimetric curve of attapulgite of the present invention and complex；
Fig. 5 is the SEM figure of attapulgite of the present invention and complex；
Fig. 6 is small intestinal mucosa histopathology histology's microexamination figure that the present invention realizes mice.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1:
The activation of attapulgite: select the attapulgite clay Ore that attapulgite crystalline content is more than 50%, carbonate content is less than 10%
Type, is ground to 200 mesh by attapulgite clay.A, take attapulgite powder, add in the beaker containing 20% hydrochloric acid, thin with plastics
Film phonograph seal, 60 DEG C of water-bath magnetic agitation 10h.B, 10000rpm are centrifugal, remove supernatant, are washed with deionized precipitation repeatedly,
Transparent to supernatant pH value to neutrality, color.C, by above-mentioned repeatedly washing after precipitation add in 10% sodium chloride solution, ultrasonic
Form suspension system；60 DEG C of water-bath magnetic agitation 10h.D, 10000rpm are centrifugal, take upper strata light gray solid, electric heating constant temperature air blast
In drying baker, 70 DEG C are dried to constant weight, cross 200 mesh sieves after grinding.
Preparing of Rhizoma Zingiberis Recens quintessence oil is as follows: take Rhizoma Zingiberis Recens, and section, crushed after being dried are cleaned in peeling.Rhizoma Zingiberis Recens powder adds 70% alcohol reflux and carries
Take 2 times, add 10 times amount for the first time, extract 90min；Add 8 times amount for 2nd time, extract 60min, merge ethanol extract, filter, filter
Liquid concentrating under reduced pressure steams ethanol to the greatest extent, obtains Rhizoma Zingiberis Recens quintessence oil.This product character is: light yellow oil, has acid, is dissolved in alcohol, water insoluble.
6-gingerol assay: use HPLC method.With dehydrated alcohol (chromatographically pure), Rhizoma Zingiberis Recens quintessence oil is dissolved, filter.At column temperature: room temperature；Stream
Dynamic phase: acetonitrile-methanol-water (40:5:55)；Flow velocity 0.8ml/min；Sample size: 20 μ l；Detection wavelength 280nm.According to standard specimen
Retention time carries out qualitative, calculates the content of 6-gingerol by external standard method.This product 6-gingerol content is 235.62mg/g.
The preparation of attapulgite Rhizoma Zingiberis Recens quintessence oil complex: take the attapulgite powder 100g after activation and add in deionized water, ultrasonic
Dispersion forms suspension system.Take Rhizoma Zingiberis Recens quintessence oil 10g to be dissolved in dehydrated alcohol, be then added in attapulgite powder suspension system, 75
Magnetic agitation 120 min at DEG C.It is precipitated after 10000rpm centrifugation, collects supernatant.Precipitation is washed with 50% ethanol solution
It is centrifuged again after washing, again collects supernatant.Precipitation after above-mentioned washing is placed in electric heating constant-temperature blowing drying box dry at 60 DEG C
Dry, ground 200 mesh sieves.Gained attapulgite Rhizoma Zingiberis Recens quintessence oil complex is pale yellow powder solid.
The preparation of powder: preparation technology prepares powder by powder.Every bag contains attapulgite Rhizoma Zingiberis Recens quintessence oil complex 2g of the present invention.
Embodiment 2:
Select the attapulgite clay Ore type that attapulgite crystalline content is more than 50%, carbonate content is less than 10%, by attapulgite
Clay abrasive is to 100 mesh.
The preparation of attapulgite Rhizoma Zingiberis Recens quintessence oil complex: take the attapulgite powder 100g after activation and add in deionized water, ultrasonic
Dispersion forms suspension system.Take Rhizoma Zingiberis Recens quintessence oil 1g to be dissolved in dehydrated alcohol, be then added in attapulgite powder suspension system, 90 DEG C
Lower magnetic agitation 150min.It is precipitated after 10000rpm centrifugation, collects supernatant.Precipitate with 50% ethanol solution washing
After be centrifuged again, again collect supernatant.Precipitation is placed in electric heating constant-temperature blowing drying box and is dried at 60 DEG C, ground 200 mesh sieves.
Gained attapulgite Rhizoma Zingiberis Recens quintessence oil complex is pale yellow powder solid.
The preparation of capsule, by capsule, preparation technology prepares capsule.Every is combined containing attapulgite Rhizoma Zingiberis Recens quintessence oil of the present invention
Thing 500mg.
The other the same as in Example 1.
Embodiment 3:
Select the attapulgite clay Ore type that attapulgite crystalline content is more than 50%, carbonate content is less than 10%, by attapulgite
Clay abrasive is to 320 mesh.
The preparation of attapulgite Rhizoma Zingiberis Recens quintessence oil complex: take the attapulgite powder 100g after activation and add in deionized water, ultrasonic
Dispersion forms suspension system.Take Rhizoma Zingiberis Recens quintessence oil 20g to be dissolved in dehydrated alcohol, be then added in attapulgite powder suspension system, 30
Magnetic agitation 180min at DEG C.Centrifugal 10000rpm is precipitated after separating, and collects supernatant.Precipitation is washed with 50% ethanol solution
It is centrifuged again after washing, again collects supernatant.Precipitation is placed in electric heating constant-temperature blowing drying box and is dried at 60 DEG C, ground 200 mesh
Sieve.Gained attapulgite Rhizoma Zingiberis Recens quintessence oil complex is pale yellow powder solid.
The preparation of powder: preparation technology prepares powder by powder.Every bag contains attapulgite Rhizoma Zingiberis Recens quintessence oil complex 2g of the present invention.
The other the same as in Example 1.
Every daily oral dose, powder instructions of taking is three times a day, each 0.5-1 bag, warm boiled water；Capsule is taken
Method is three times a day, each 2-4 grain.It within 7 days, it is a course for the treatment of.
The oral formulations made in order to top method, carries out pharmacodynamics test and the safety evaluatio test being correlated with.It is presented herein below
The animal experimental data of the present invention:
The preparation of escherichia coli bacteria suspension: be inoculated in by escherichia coli in LB culture medium, 37 DEG C of gas bath constant temperature oscillators vibrate
After cultivating 12 h, it is precipitated centrifugal for fluid medium, rinses bacterium colony 2 times with sterile saline, after being centrifuged, use sterilizing again
Normal saline makes escherichia coli bacteria suspension, is 2 × 10 according to colony counting method compound concentration respectively7CFU/mL、2×108CFU/
mL、2×109 CFU/mL、2×1010CFU/mL bacterial suspension, 4 DEG C save backup.
Set up coli-infection animal diarrhea model: 12h fasting before mouse experiment, freely drink water.Lumbar injection is corresponding
The bacterium solution of concentration, volume injected is 0.5mL, the isopyknic physiological saline solution of matched group lumbar injection.Mice occurs significantly
Symptom of diarrhea, watery stool, lethargy, be slow in action etc. shows that model is successfully established.
EXPERIMENTAL DESIGN: mice 80, male and female half and half, body weight 30g ± 2g, divides 8 groups, often group 10, respectively normal control
Group, model control group, positive controls, high dose group of the present invention, middle dosage group, low dose group, attapulgite group, Rhizoma Zingiberis Recens essence line of oils.
After positive controls mice modeling, 4h oral administration of montmorillonite powder dissipates 1g/kg, high dose group of the present invention, middle dosage group, low dose group mice
After modeling 4h be administered orally attapulgite Rhizoma Zingiberis Recens quintessence oil complex of the present invention 600,300,100mg/kg, 4h mouth after attapulgite group mice modeling
Take attapulgite 600mg/kg.After Rhizoma Zingiberis Recens essence line of oils mice modeling, 4h is administered orally Rhizoma Zingiberis Recens quintessence oil 125mg/kg.Every day 1 time, continuous 6 days.
Testing index:
Every day, timing weighed each group of Mouse Weight, and calculated mice food ration.
Diarrhoea index: mice is put into the mouse cage being covered with filter paper, observes diarrhea of mouse situation.Calculating diarrhea rate: often group
In animal, the number of animals of passage of loose stools accounts for the percentage ratio of this treated animal sum.
Pathologic examination: take small intestine, formalin solution is fixed, and routine is drawn materials, and series of ethanol is dehydrated, paraffin
Conventional film-making (4 μm) after embedding, HE dyes, and observes small intestinal mucosa changes in histopathology under an optical microscope and takes the photograph sheet (200
×).
Results of animal is as shown in following table 2-4:
The table 2 impact on Mouse Weight
The table 3 impact on mice food ration
The table 4 impact on diarrhea of mouse rate
Result shows, compared with Normal group, model control group Mouse Weight, food ration substantially reduce, and diarrhea rate substantially rises
High.Attapulgite Rhizoma Zingiberis Recens quintessence oil complex of the present invention can substantially recover body weight and the food ration of diarrhea mice, reduces diarrhea rate, promotes
The rehabilitation of mice.Fig. 6 pathologic examination result shows, A Normal group in figure；B model control group；C positive controls；
D low dose group of the present invention；Dosage group in the E present invention；F high dose group of the present invention；G attapulgite group；H Rhizoma Zingiberis Recens essence line of oils；Under microscope
The observing and nursing group obvious atrophy of mouse small intestine fine hair, rupturing, come off, goblet cell increasing number, fluff tip expands and fine hair
Base portion reduces, and vacuolation is notable, and epithelial cell substantially comes off；Of the present invention group of mouse small intestine fluff structures is clear, and marshalling is tight
Close, mucosa is complete, and epithelial cell completely compacts.Illustrate that the present invention has recuperating gastrointestinal tract function, antimicrobial antiphlogistic, has intestinal mucosa
There is significant protective effect；And effect to be better than positive control, attapulgite or Rhizoma Zingiberis Recens quintessence oil alone.Attapulgite and Rhizoma Zingiberis Recens quintessence oil are described
Compound have the collaborative effect strengthening drug effect.
The present invention shows through the acute toxicity tests, and mouse stomach to the present invention one day maximum dosage-feeding (MTD) is
21.5g/kg, is equivalent to effective dose 72 times；There is not any untoward reaction in animal.Product safety of the present invention is described, almost without
Toxicity.
Claims (4)
1. one kind has the complex improving functional gastrointestinal disorder effect, it is characterised in that: include attapulgite powder and Rhizoma Zingiberis Recens essence
Oil, described attapulgite powder is 10:0.1-2 with the mass ratio of Rhizoma Zingiberis Recens quintessence oil.
The most according to claim 1 a kind of have the complex improving functional gastrointestinal disorder effect, it is characterised in that institute
State attapulgite powder, select the attapulgite clay Ore type that attapulgite crystalline content is more than 50%, carbonate content is less than 10%,
Attapulgite clay is ground to 100-320 mesh.
The most according to claim 1 a kind of have the complex improving functional gastrointestinal disorder effect, it is characterised in that institute
Stating Rhizoma Zingiberis Recens quintessence oil to be extracted by Rhizoma Zingiberis Recens, wherein gingerol content is more than 20%.
The most according to claim 1 a kind of have the complex improving functional gastrointestinal disorder effect, it is characterised in that recessed
Convex rod Rhizoma Zingiberis Recens quintessence oil complex is mixed and made into powder or capsule with excipient.
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