KR101192280B1 - The preparing method of candy for young children using nano-encapsulated Angelica gigas for enhancing immune activity, and the candy - Google Patents

Abstract

The present invention Angelica ( Angelica) gigas ) relates to a method for preparing immune-promoting candy for children using nanoparticles of water-soluble extracts.
According to the present invention, a method for preparing a pediatric immune-promoting candy using nanoparticles of water-soluble extract of Angelica gigas is characterized in that it is produced by gelatinizing the hydrothermal extract of Angelica gigas.
According to the present invention, due to the effective cell permeation function of the nanoparticle granulated Angelica extract, high activity can be expected even with a small concentration of extract, which is economical in terms of cost and time. In addition, the bitter Angelica is collected by gelatin and made into a candy, which can be easily consumed by children by reducing the inherent odor and enhancing flavor.

Description

The preparation method of candy for young children using nano-encapsulated Angelica gigas for enhancing immune activity, and the candy}

The present invention Angelica ( Angelica) gigas ) relates to a method for preparing immune-promoting candy for children using nanoparticles of water-soluble extracts.

Recently, as interest in efficient body delivery of herbal medicines has increased, the scope of research on herbal materials has expanded, and interest in improving immune system in the body in preparation for various immune diseases such as diabetes, obesity, hyperlipidemia, and cancer has been amplified. As it is, the present invention is characterized in that the nano-particles in the appropriate method and means to nanoparticles to effectively deliver the useful herbal medicine to children.

In other words, the present invention is to differentiate the conventional candy manufacturing method by using the gelatin material as an edible material, and added by adding nano-particles of the Angelica Angelica extract to 100 ~ 200nm, to enhance the value of the candy as an immune-enhancing food. Since the present candy composition using the herbal medicine has the advantage of the intracellular delivery route through which gelatin nanoparticles can show great activity in the body even with a small extract, the existing economic problem of supplying the body by increasing the amount of the extract is known. It has improved physiological and synergistic effects of gelatin, effectively enhancing immune activity. In addition, the nanoparticles make the extract polarized (polarity) can be stably penetrated into the digestive system.

Angelica Angelica gigas ) is a perennial herb belonging to the family Apiaceae, which has an immunomodulatory effect and is an effective herbal medicine for fragile constitution. Since ancient times, it has been widely used as a blood donor and a blood pill for gynecological diseases such as sedation, analgesic, anemia and dysmenorrhea and hypothermia.

Angelica is native to deep mountains but is cultivated mainly. Stems are about 1 ~ 2m high and roots are used as drugs. Roots are about 20 ~ 25㎝ long and 1 ~ 5㎝ in diameter, containing milk and fragrance. strong. The Korean Pharmacopoeia introduces the fact that as a weakness of donkey, 'it smells sweet and tastes slightly sweet', but it can be felt only in Chinese donkey and Korean donkey.

Champagne don't taste sweet, only bitter. The coumarin derivatives decusin, decursinol and umbelliferon have been reported. Recently, antimutagenic activity, cancer cell growth inhibitory activity, and liver detoxification activity have been reported. Recently, as raw materials of functional food materials, tablets, pouches, and the like have been developed, and a lot of researches have been made on the new use thereof.

Champagne Angelica contains a lot of active ingredients that have an immune enhancing function, but its disadvantages are that its active ingredients are not effectively absorbed through digestive mechanisms, and have a bitter taste without a sweet taste. Because of this, children's intake is limited. The present invention is to provide a method for producing a candy composition capable of enhancing bioavailability for immuno-promoting by nano-particles of water-soluble Angelica extract using edible gelatin to improve this.

Candy is a candy made by boiling sugar in various shapes, such as round egg shaped candy, candy or sugar, boiled hard candy, and sugar mixed with fruit juice or spices and boiled in various shapes and colors. . It is usually made by sifting sugar into a mold and applying heat to it, which is relatively simple and easy to manufacture compared to other foods. However, compared to the easy manufacturing process, the syrup type and the composition of the material show a considerable difference in physical properties, and the combination and manufacturing method may cause syneresis, which causes quality deterioration during candy distribution, to prevent this. Several methods are being studied. Also disclosed in the country are candy containing pulp, candy prepared with diet foods, hard candy without sugar, candy with increased moisture content, herbal shake aroma candy, and the like.

Nanotechnology of the present inventors is a technology that can create a new new material through the manipulation of atomic and molecular units to a size of 1 billion / 1m. Recently, nano technology has attracted attention as a technology that anyone can easily access as products such as yogurt, nano cosmetics, silver nano appliances, and nano pesticides using nano particles are released in large quantities. In particular, nanoparticles are hundreds of times smaller than our human cells, so when we combine nanotechnology with natural extracts, quantum-mechanical approaches that traditional food science has overlooked are possible, and we can deliver useful substances stably.

The gelatin selected by the inventors of the present invention is a pure protein food which is not toxic in vivo and is easily digested, and is currently used in various functional foods, cosmetics, ointments, tablets, and pharmaceutical industries for producing products related to plasma. Gelatin can easily collect water-soluble, fat-soluble physiologically active substances, has the advantage that it can be used as a solvent, external preparation. Gelatin in the form of granules without additives has almost no taste and odor, and the color is very pale yellow. Gelatin is used in the form of granules and mixed with sugar, flavor, acid and pigment, and it is stable for a long time when stored in a sealed container at room temperature.

It is an object of the present invention to provide a method for preparing a pediatric candy having immune function enhancing function through gelatin nanoparticles of Angelica gigas.

More specifically, the present invention by nano-particles of Angelica gigas widely used in herbal medicine by using gelatin material, which is an edible material, overcomes the difficulty of children ingesting Angelica Angelica due to its bitter taste and absorbs the active ingredient well in the body even after ingestion. The purpose is to make it possible.

In the present invention, by improving the problems that the candy containing the existing natural extract has to improve the temperature, pH, body absorption stability of the candy through nanoparticles. In addition, by incorporating some of the technologies related to nano drug carriers into the Chinese herbal medicine, Angelica, it is intended to overcome barriers at the level of cell delivery system of existing extracts, and to re-evaluate the effects of candy as a functional food for immune enhancement. The effective cell permeation function of nanoparticle granulated Angelica extract is economical in terms of cost and time since high activity can be expected even with a small concentration of extract. In addition, the bitter Angelica is collected by gelatin and made into a candy, which can be easily consumed by children by reducing the inherent odor and enhancing flavor. This research is the only technology possessed by the researchers in Korea because no research has been conducted on nano-foods that have bioimmune enhancing effects of natural medicines collected with gelatin.

According to the present invention, a method for preparing a pediatric immune-promoting candy using nanoparticles of water-soluble extract of Angelica gigas is characterized in that it is produced by gelatinizing the hydrothermal extract of Angelica gigas.

More specifically, the manufacturing method of the present invention,

(1) crushing the Angelica Angelica;

(2) extracting the pulverized Angelica Angelica using low-temperature hot water for 24 hours at 100 ° C. using 10-fold distilled water as a solvent;

(3) filtering and concentrating the hot water extract with a vacuum filter, and then lyophilizing the powder into powder;

(4) dissolving the above powder in distilled water at a concentration of 1 mg / ml;

(5) dissolving the powdered gelatin in distilled water in a container separately from the above-mentioned steps, and then distilled water is evaporated using a pressure reducer to form a multilayer;

(6) putting the liquid Angelica extract sample of (4) into a container of (5), which was completely dried and layered, and homogenized with an ultrasonic disperser to prepare water-soluble nanoparticles; And

(7) 7 wt% of Angelica gigas nanoparticles prepared as described above, 80 wt% oligosaccharide, 11 wt% xylitol, 2 wt% citric acid, characterized in that the step of mixing and stirring.

The present invention is also characterized in that the pediatric immunity enhancing candy produced by each of the above production methods.

 In the present invention, by using gelatin to prepare the extract of Angelica Angelica as a fine nanoparticles in the form of liposomes of 100-200 nm size, it is easy to permeate and remain in the intestinal tract and can overcome the permeation barrier caused by the cell gap of 200 nm size. I want to be.

To this end, 7% by weight of the Angelica extract nanoparticles gelatinized in gelatin in a candy composition containing the syrup prepared sweetened as the main ingredient is administered.

When making a candy is usually made by adding sugar to the mold heat is used in the present invention oligosaccharides instead of sugar. Oligosaccharides are sweet like sugar, but only about a quarter of the calories are effective in preventing obesity. Therefore, the sugar used in the conventional candy manufacturing process is directly absorbed into the blood vessels to make the human body rapidly hyperglycemic state, while the oligosaccharide used in the present invention stabilizes insulin secretion due to slow absorption in the body. In addition, unlike starch syrup, which causes the bleaching process to be a problem, it inhibits the production of harmful substances in the intestine and acts as a suit and prevents tooth decay. Since oligosaccharides have a sweet taste of about 50% of the sugar, in the present invention, 80% by weight of the total weight was added to the candy composition.

According to the present invention, due to the effective cell permeation function of the nanoparticle granulated Angelica extract, high activity can be expected even with a small concentration of extract, which is economical in terms of cost and time. In addition, the bitter Angelica is collected by gelatin and made into a candy, which can be easily consumed by children by reducing the inherent odor and enhancing flavor.

The inventors of the present invention have examined the difference between nano-particles of edible donkeys with edible gelatin and compared with the extracts of conventional donkeys through a conventional extraction process, through several immunological and sensory evaluation experiments. In addition, by improving the pH instability of the existing Angelica extract, problems of sticky physical properties due to sugar at an appropriate temperature, and problems that children could not enjoy due to bitter taste, it can be developed into a more pleasant food Determined whether or not.

Hereinafter, the process of the present invention will be described in detail through the following Examples.

[Step 1: Sample Preparation Process]

The raw or dried domestic Angelica gigas to be extracted are ground to an appropriate size and immersed in distilled water at a predetermined weight ratio (usually 10 times) in an extraction flask attached to a vertical reflux cooler to extract hot water at 100 ° C.

After the extraction is completed, the solvent and the extract are separated, and the extract is obtained as a powder by filtration and concentration under reduced pressure. The powdery sample is dissolved in distilled water at a concentration of 1 mg / ml.

[Step 2: Nanoparticles]

In order to explore the bioavailability enhancing effect of nanoparticles, the Angelica gigas extracts are prepared with nanocomposite extract and biocompatible gelatin material.

In a round bottom flask, gelatin on powder, an edible material, is dissolved in warm distilled water at 40 ~ 50 ℃ to form a multilayer using a pressure reducer. The liquid donkey extract sample prepared in the first step was put into a round bottomed flask formed with a layer completely dried and homogenized at 25-27 ° C. for 2 hours at room temperature using an ultrasonic disperser to obtain water-soluble nanoparticles. Manufacture.

Once the nanoparticles are produced, they are stored in a glass jar to prevent denaturation of the nanoparticles due to the charge difference.

[Step 3: Obtaining Extract and Measuring Nanoparticle Size]

The production and size of liquid edible true Angelica nanoparticles are measured using dynamic light scattering (DLS).

[Step 4: Preparation of Candy after Mixing Candy Base and Angelica Nanoparticles]

The object of the present invention as described above can be achieved by mixing the nanoparticles prepared by the second and third processes in the candy base. 93 wt% of candy base is mixed with 7 wt% of Angelica gigas nanoparticles with stirring at 100-120 ° C. for 1 hour. Candy base is 80% oligosaccharide by weight, 11% xylitol (polyol), 2% citric acid. By such a component and content, oligosaccharides and xylitol can have a crystallization effect and increase the coating speed of the candy. After mixing, vacuum concentration, cooling, etc. are performed (see Table 1 below).

Table 1. Overall flow table of the process

Hereinafter, the present invention will be described in more detail with reference to specific examples.

Example 1.

Raw or dried domestic Angelica obtained by grinding for the examples of this experiment was immersed in 10 times (w / v) distilled water with respect to 50g of sample weight in an extraction flask attached to a vertical reflux condenser for 24 hours at 100 ℃. During hydrothermal extraction. An extract obtained by using 10 times of distilled water as a solvent was concentrated on a Angelica Angelica sample, and concentrated by filtration with a rotary vacuum evaporator (N-N series, EYELA), followed by lyophilization to obtain a powder.

In order to explore the bioavailability enhancement effect of the sample nanoparticles gelatin gelatin edible food and various physiological activities, nanoparticles (nanoparticles) were prepared using the extract of Angelica gigas. To this end, 50 mg of 100 ° C. distilled water extract sample was dissolved in distilled water at a concentration of 1 mg / ml. Separately, gelatin was dissolved in warm distilled water at 40-50 ° C. in a round bottom flask to form a multilayer using a pressure reducer. A liquid donkey extract sample was placed in a round bottomed flask having a completely dried layer, and homogenized under conditions of Amp 38% and pulse 7: 5 for 2 hours at 26 ° C. using an ultrasonic disperser to prepare water-soluble nanoparticles.

Example 2.

In order to grasp the structure of the formed nanoparticles, the present inventors observed using a transmission electron microscope, which is a principle of enlarging and observing electrons passing through an electron beam by shooting an electron beam onto a target sample. As a result, it was confirmed that the Angelica nanoparticles collected with gelatin were stably distributed in the liquid phase. In order to prepare nanoparticles of a size that can be effectively absorbed into the body, each size generated by dynamic light scattering (DLS) was measured.

The size analysis of the nanoparticles shows that when nanoparticles are made of edible gelatin, an average particle size of 116.9 nm is produced, which can be the most optimal condition for skin cell permeation. In addition, when the nanoparticles are gelatinized by gelatin through the TEM image, it is thought to be effective in collecting a large amount of Angelica gigas extract in the intestinal tract (see above picture 1, graph 1, table 2).

Example 3.

The inventors of the present invention in order to examine the effect of improving the physiological activity through the increase of the yield of the extract according to the present invention, the two Angelica extract (① 100 ℃ hot water extract with distilled water as a solvent, ② collected by gelatin) Nanoparticles) was used for the cytotoxicity test of the extract. In this experiment, normal cytotoxicity of HEK293 (normal, human, KCLB, KOREA), human normal kidney cells, was investigated by the addition of 1 mg / mL sample. SRB assay was used for cytotoxicity measurement. Normal cell toxicity of the samples was measured by measuring the growth of HEK293 cells and comparing with the untreated control.

As a result, the normal renal cytotoxicity of gelatin-collected nanoparticles was recorded lower than that of Angelica gigas extract by hot water extraction. When liposome liposomes were formed with gelatin, a functional material that is harmless to living organisms, cytotoxicity was less than 12% at the highest concentration of 1mg / ml. It is believed to be biocompatible as the material of the functional candy composition for promotion (graph 2).

Example 4.

In order to confirm the immune-promoting effect on B cells, which play an important role in the human immune system and anticancer, 6, according to the addition of true Angelica extract (① 100 ℃ hot water extract using distilled water, ② nanoparticles collected with gelatin) The effect of promoting the growth of immune cells during the day was examined.

As a result, it was confirmed that gelatin-collected nanoparticles increased more than 12 times in terms of promoting growth of immune B cells (Graph 3). It is thought that high growth rate appeared due to the rapid penetration into the cell through nanoparticles, and B cell growth occurred effectively because the gelatin material itself contains components that enhance the growth of immune cells.

Example 5.

In order to understand the texture characteristics according to the level of addition of nanoparticle candy, physical property tests such as hardness, thermal stability, and color were conducted.

A mixture of candy raw materials consisting of 80% oligosaccharide, 11% xylitol, and 2% citric acid as the main raw materials, was added using a fraction collector so that a uniform amount was added for 1 hour while continuously stirring and heating at 100 ° C. Nanoparticles were added to 7% of the total weight. At this time, the concentration of donkey nanoparticles was set to three different values, such as 0.2mg / ml, 1mg / ml, and 1.8mg / ml (only when the powder samples were dissolved in distilled water and all other manufacturing processes were the same). Was carried out. The mixture was prepared by stirring for 1 hour at room temperature to prepare a natural Angelica nanoparticle candy. As a control, the ratio of the base was 80% oligosaccharide, 11% xylitol, 2% citric acid, and 7% distilled water was added instead of the nanosample, and other conditions were prepared using the same candy.

As a result of texture evaluation, it was confirmed that the 1 mg / ml nano-sampled candy showed stability even at a hardness of 3.7 kg and a high temperature of 60 ° C or higher.

In addition, thirty adults were evaluated for the flavor by using the intensity of smell and taste as 1-5 points. As a result, the average score of 2 points in the 1mg / ml nano sample addition group is expected to allow children to consume candy without feeling the bitterness or strong smell of Dongguk.

The stability of the candy was evaluated at pH 2-8, similar to the main digestive system. As a result, it was confirmed in Fig. 6 that the nanoparticles remained stable without breaking even at pH 2. Since the difference in size of the nanoparticles shows a difference of 30 nm or less at pH 2, the distribution and size can be said to be stable.

In addition, in the 1mg / ml nano sample addition group, Angelica's unique natural color entered the candy and was soft (see Table 3 and Graph 4 above).

Claims (3)

Characterized in that by preparing the nanoparticles of hydrothermal extract of Angelica gigas with gelatin,
Method for producing immunosuppressive candy for children using nanoparticles of Angelica gigas hot water extract. (1) crushing the Angelica Angelica;
(2) extracting the pulverized Angelica Angelica using low-temperature hot water for 24 hours at 100 ° C. using 10-fold distilled water as a solvent;
(3) filtering and concentrating the hot water extract with a vacuum filter, and then lyophilizing the powder into powder;
(4) dissolving the above powder in distilled water at a concentration of 1 mg / ml;
(5) dissolving the powdered gelatin in distilled water in a container separately from the above-mentioned steps, and then distilled water is evaporated using a pressure reducer to form a multilayer;
(6) putting the liquid Angelica extract sample of (4) into a container of (5), which was completely dried and layered, and homogenized with an ultrasonic disperser to prepare water-soluble nanoparticles; And
(7) characterized in that the step of mixing, stirring and solidifying 80% by weight of oligosaccharide, 11% by weight of xylitol, 2% by weight of citric acid to 7% by weight of Angelica gigas nanoparticles prepared as described above,
Method for producing immunosuppressive candy for children using nanoparticles of Angelica gigas hot water extract. It is produced by the manufacturing method of claim 1 or 2,
Pediatric Immune Enhancement Candy Using Nanoparticles of Angelica gigas Hot Water Extract.