CN109771457A - Purposes of the russule extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease - Google Patents
Purposes of the russule extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease Download PDFInfo
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Abstract
The present invention relates to a kind of fungi extracts in the purposes of purposes more particularly to russule extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease.Of the invention preparation method is simple, operating cost is low, Extraction solvent in preparation method of the invention can remain risk with recycling and reusing, no organic reagent, it is while ensure that the natural quality of russule extract and environmentally friendly, belong to green manufacture technology.Raw material extraction of the present invention is more complete, takes full advantage of raw material, raw material availability is also very high on the basis of guaranteeing extract effect.Russule extract pair disease relevant to hepatic injury shows significantly treatment and/or preventive effect, such as the deterioration of inflammation can be effectively relieved, improve immune-related every physiopathological index, can be used in the various fields such as drug, health care product, there is great economy and society value.
Description
Technical field
The present invention relates to a kind of fungi extracts to cause hepatic injury related disease in preparation treatment and/or prevention high fat diet
Preparation in purposes more particularly to russule extract preparation treatment and/or prevention high fat diet cause hepatic injury related disease
Preparation in purposes.
Background technique
Enteric microorganism group is known as " second set of genome of human body ", closely bound up with human health.The micro- life of human intestine
Up to 1000 kinds or more of species, wherein Firmicutes and Bacteroidetes are dominant microflora, the gene dosage in enteron aisle genome
It is more than 100 times of mankind's autogene group.There is mutualism relationships by intestinal flora and host, and dynamic is remain in enteron aisle
Balance, when balance is broken, people is possible to suffer from various diseases.Existing research confirms: (1) intestinal flora and fat phase
It closes, there are intestinal microflora imbalance, Phylogenetic diversity of bacteria declines for obese people；The Bacteroidetes of obesity mice is few, Firmicutes
It is more；The enteric microorganism of obesity mice is transplanted on germfree mouse, will lead to the increase of the latter's fat mass.Fat people is come
It says, takes in high fat diet for a long time, the change of intestinal microflora can be caused, unbalance intestinal flora can generate endotoxin and enter people
Body generates multiple inflammatory factors after being identified by immunocyte, and body is caused to be in low grade inflammation state, generates metabolic disorder, makes
It obtains the energy absorbed from food and is easily converted to fat, cause obesity；(2) liver and enteron aisle are close on biological function
It is related, interactional.Liver and intestinal flora are interacted by intestines-liver axis, and intestinal flora is close with liver diseases, liver
When illness, the structure and function of intestinal flora will affect, and can influence liver function when intestinal bacilli illness in turn, liver is hard
Changing the beneficial bacteriums quantity such as patient Bifidobacterium reduces, and the harmful bacterias quantity such as enterobacteria, enterococcus increases.
High fat diet can change intestinal microflora, reduce beneficial bacterium, and harmful bacteria increases, and destroy the flat of intestinal flora
Weighing apparatus, and then liver function is influenced, hepatic injury is caused, and increasing the problems such as with the development and dietary structure of China's living standard
Add, the disease incidence of the hepatic injury as caused by high fat diet obviously increases, and there is an urgent need to develop treat and/or prevent high in fat in preparation
Diet causes the preparation in terms of hepatic injury related disease.
Summary of the invention
Against the above deficiency, the present invention provides a kind of russule extract and causes liver in preparation treatment and/or prevention high fat diet
Damage related disease preparation in purposes, function is significant clear, can medicinalization, have practicability.
The present invention reaches above-mentioned purpose by following scheme:
In the first aspect of the present invention, a kind of russule extract is provided treating and/or prevent high fat diet and causes hepatic injury
Purposes in related disease.
In the second aspect of the present invention, a kind of russule extract is provided in preparation treatment and/or prevention high fat diet and causes liver
Damage the purposes in the preparation of related disease.
In the third aspect of the present invention, a kind of system treating and/or preventing high fat diet and cause hepatic injury related disease is provided
Agent, the preparation include russule extract.
In the fourth aspect of the present invention, a kind of medicine treating and/or preventing high fat diet and cause hepatic injury related disease is provided
Object includes russule extract and pharmaceutically acceptable carrier.
In the fifth aspect of the invention, a kind of preparation method of russule extract is provided, comprising: crush russule fructification
Afterwards, it is successively mentioned with alcohol extracting, water, the extraction of NaOH buck, respectively obtains extraction solution, then concentrated, freeze-drying respectively, obtained red
Mushroom alcohol extracting thing, russule water extract, russule alkali extract.
Compared with prior art, the invention has the following beneficial effects:
(1) of the invention preparation method is simple, operating cost is low, and the Extraction solvent in preparation method of the invention is only
Ethyl alcohol, water, NaOH buck have been used, risk can have been remained with recycling and reusing, no organic reagent, ensure that the day of russule extract
It is while right quality and environmentally friendly, belong to green manufacture technology.
(2) present invention successively extracts russule fructification using ethyl alcohol, water and NaOH buck, and raw material extraction is more complete, sufficiently
Raw material is utilized, raw material availability is also very high on the basis of guaranteeing extract effect.
(3) russule extract pair of the invention disease relevant to hepatic injury shows significantly treatment and/or pre- preventive effect
Fruit, such as the deterioration of inflammation can be effectively relieved, improve immune-related every physiopathological index, can be used for drug, health care
In the various fields such as product, there is great economy and society value.
Detailed description of the invention
Fig. 1 is the small intestine HE coloring pathological section figure of embodiment 2.
Fig. 2 is the liver organization pathological section figure of embodiment 2.
Specific embodiment
In the first aspect of the present invention, a kind of russule extract is provided treating and/or prevent high fat diet and causes hepatic injury
Purposes in related disease.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the second aspect of the present invention, a kind of russule extract is provided in preparation treatment and/or prevention high fat diet and causes liver
Damage the purposes in the preparation of related disease.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the third aspect of the present invention, the preparation of a kind of prevention and/or treatment hepatic injury related disease, the preparation includes
Russule extract.
Preferably, the preparation includes drug, health care product or food.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the fourth aspect of the present invention, a kind of drug prevented and/or treat hepatic injury related disease is extracted comprising russule
Object and pharmaceutically acceptable carrier.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
Heretofore described " pharmaceutically acceptable carrier " is being cured it is also understood that for " diluent " or " auxiliary material " etc.
It is known that and being described in such as Remington medical science (Remington ' s Pharmaceutical in medicine field
Sciences), Mack Publishing Company (Mack Publishing Co.) (AR lattice nano (A.R.Gennaro) compile,
1985).These substances are nontoxic for recipient under used dosage and concentration, including such as phosphate, citric acid
The buffers such as salt, acetate and other acylates；The antioxidants such as ascorbic acid；The low molecular weights such as poly arginine
(being less than about 10 residues) peptide；The protein such as seralbumin, gelatin or immunoglobulin；Such as polyvinylpyrrolidone
Equal hydrophilic polymers；The amino acid such as glycine, glutamic acid, aspartic acid or arginine；Monosaccharide, disaccharides and including cellulose
Or derivatives thereof, other carbohydrate of glucose, mannose or dextrin；The chelating agents such as EDTA；Such as mannitol or
The sugar alcohols such as sorbierite；The counter ion counterionsl gegenions such as sodium；And/or such as tween (Tween), Pluronic (Pluronics) or poly- second
The nonionic surface active agent such as glycol；Further including includes sterile water, salt water, glucose, oil (such as corn oil, peanut oil and class
Like object), acid.Further can also include preservative, wetting agent, emulsifier, and the like etc..
The dosage of russule extract or drug of the invention can with specific medication and treated object or
Type and change.The dosage is enough to generate expected beneficial effect, and can be formulated for single dose application or multiple dose
Amount application.
Heretofore described drug can be applied by any approach well known by persons skilled in the art, including oral,
The approach such as intramuscular, intravenous, intradermal, intralesional.Application can be part, surface or whole body.In any given feelings
Most suitable approach depends on many factors, such as property, the process of disease, the seriousness of disease of disease etc. under condition.As
It is preferred that russule extract of the invention or being administered orally comprising the drug including russule extract.
The russule extract of the invention can also be applied together with the second reactive compound/second therapeutic agent, simultaneously,
It applies in succession or parallel.When applying respectively, the russule extract of the invention or the drug comprising russule extract and
Two reactive compounds or second therapeutic agent can be applied with different medicine frequencies or interval.
In the purposes of aforementioned four aspect of the invention or preparation, the russule extract include russule water extract,
Russule alcohol extracting thing, russule alkali extract.
In one embodiment, the russule alcohol extracting thing is russule ethanol extract.
In one embodiment, the russule alkali extract is russule sodium hydroxide extract.
Preferably, in the purposes of aforementioned four aspect of the invention or preparation, the russule extract is preferably following
The russule extract that preparation method is prepared.
In the fifth aspect of the invention, a kind of preparation method of russule extract is provided, comprising: crush russule fructification
Afterwards, it is successively mentioned with alcohol extracting, water, the extraction of sodium hydroxide (NaOH aqueous solution) buck, respectively obtains extraction solution, then respectively through dense
Contracting, freeze-drying, obtain russule alcohol extracting thing, russule water extract, russule alkali extract.
Preferably, a kind of preparation method of russule extract, comprising: after crushing russule fructification, successively with 95% second
Alcohol extracting, water are extracted, 1%-2%NaOH buck extracts, and respectively obtain extraction solution, then concentrated, freeze-drying respectively, are obtained
Russule alcohol extracting thing, russule water extract, russule alkali extract.
Preferably, a kind of preparation method of russule extract, comprising: after crushing russule fructification, using alcohol reflux
It extracts, obtains the first filter residue and alcohol extracting solution, the first filter residue is used into water refluxing extraction solution, the second filter residue is obtained and water mentions
Solution is taken, then the second filter residue is used into 1%-2%NaOH buck refluxing extraction, third filter residue is obtained and alkali carries takes solution, by alcohol
It extracts solution, water extraction solution and obtains russule alcohol extracting thing, russule water extract by concentration, freeze-drying respectively；By alkali carries
Solution is taken to obtain russule alkali extract by concentration, alcohol precipitation, freeze-drying.
Preferably, it includes: that the ethyl alcohol that solid-liquid ratio is 1:8-1:15 is added that the alcohol reflux, which extracts, and refluxing extraction 2-4 is small
When, it filters, obtains filtrate, is concentrated under reduced pressure to give thick paste, -80 DEG C of freeze overnights of thick paste warp are freeze-dried to get russule alcohol extracting
Object.
Preferably, the water refluxing extraction includes: the filter residue after taking alcohol extracting, uses solid-liquid ratio for the water of 1:8-1:15, is returned
Stream extracts 1-3 hours, filters, obtains filtrate, is concentrated under reduced pressure to give thick paste, -80 DEG C of freeze overnights of thick paste warp, freeze-drying, i.e.,
Obtain russule water extract.
Preferably, the 1%-2%NaOH buck extract include: learn from else's experience alcohol and water mention after filter residue, use solid-liquid ratio for
The buck of 1:8-1:15, stirring is ultrasonic extraction 1-2 hours lower, filters, and obtains filtrate, and alcohol reagent precipitating is added, precipitates 12-24
Hour, precipitating is collected, through -80 DEG C of freeze overnights, is freeze-dried to get russule alkali carries object.
The 1%-2%NaOH or the 1%-2%NaOH buck refer to that weight percentage is the NaOH water of 1%-2%
Solution.
Preferably, the 1%-2%NaOH is 2%NaOH.
It is further preferred that the alcohol reagent of the precipitating includes ethyl alcohol, methanol.
It is further preferred that described be precipitated as that 4-6 times of the liquid ethyl alcohol measured is added, precipitate 12-24 hours.
In the sixth aspect of the present invention, a kind of russule extract that above-mentioned preparation method is prepared is provided.
Russule (Russula vinosa Lindblad), fructification are general medium big.It is 5-12 centimetres of bacteria cover diameter, just flat
It is open and flat after hemispherical, it is glued when young, matt or velvet-like, middle part color depth is red to dark (black) red, and thin edge is in peony, Gai Yuanchang
See thin band.Bacterial context white, it is thick, often eaten by worm.Taste and smell are good.Lamella white, becomes milk yellow after old, can at nearly lid edge
Band is red, slightly close to slightly dilute, often has bifurcated, has transverse vein between pleat.Wild Russula vinosa fecund is in Hunan Yanling County, Nanyang, henan, Guizhou
The ground such as the southeast of Guizhou Province, Fujian, Jiangxi, Guangdong, Guangxi, Sichuan, Shaanxi, Yunnan, Hubei.Foreign countries be distributed in Vietnam, Burma, Laos,
The ground such as Cambodia.Russule unique flavor, spiciness are tasty and refreshing.Its taste compared with any mushroom class can not rival it is fresh and sweet palatable；And contain someone
The ingredients such as required a variety of amino acid.With treatment tumour especially lung tumors, waist and leg ache, numb in every limb, muscles and bones is uncomfortable, four
The diseases such as limb twitch and blood enriching and yin nourishing, refreshing and detoxicating and treatment anaemia, oedema, malnutrition and puerpera's Massive Bleeding, also have
The effects of increasing immunity of organisms and anticancer, it is often edible, application on human skin can be made fine and glossy, it is full of vitality, it prolongs life.
Heretofore described " imbalance " can be understood as " unbalance ", " disorder " or " disease " etc..
As used herein, term "comprising" or " comprising " refer to that method includes cited element, but are not excluded for other
Element.
Russula Basidiomycetes of the present invention is a kind of fungi, contains 5 kinds of polysaccharide, 16 kinds of amino acid and 28 kinds of rouge
Fat acid.This edible mushroom value is high.
Below in conjunction with specific embodiment, invention is further explained.
Embodiment 1: the preparation of russule extract, steps are as follows:
(1) russule fructification is crushed, uses solid-liquid ratio for the ethyl alcohol of 1:8-1:15, refluxing extraction 2-4 hours, filtered,
The thick paste for obtaining filtrate, being concentrated under reduced pressure to give debita spissitudo, -80 DEG C of freeze overnights of thick paste warp, enter freeze drier it is dry to get
Russule alcohol extracting thing.
(2) the above-mentioned filter residue after alcohol extracting is taken, uses solid-liquid ratio for the water of 1:8-1:15, refluxing extraction 1-3 hours, is taken out
Filter, the thick paste for obtaining filtrate, being concentrated under reduced pressure to give debita spissitudo, -80 DEG C of freeze overnights of thick paste warp enter freeze drier drying,
Up to russule water extract.
(3) the above-mentioned filter residue after alcohol and water mentions is taken, uses solid-liquid ratio for the 2%NaOH buck of 1:8-1:15, under stirring
It ultrasonic extraction 1-2 hours, filters, obtains filtrate, debita spissitudo ethyl alcohol is added, precipitate 12-24 hours；Precipitating is collected, through -80 DEG C
It is dry to get russule buck extract to enter freeze drier for freeze overnight.
Obtained russule alcohol extracting thing, russule water extract, russule buck extract are subjected to determination of polysaccharide, polysaccharide
Assay uses People's Republic of China's agricultural industry criteria NY/T 1676-2008 " survey of Thick many candies content in edible mushroom
It is fixed " method；Protein content determination uses Kjeldahl's method.
Thick many candies and protein content in 1 russule extract of table
Embodiment 2: improvement result of the russule extract to high fat diet obesity-induced mice hepatic injury
1, reagent:
Total cholesterol (T-CHO) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Triglycerides (TG) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Low density lipoprotein cholesterol (LDL-C) testing cassete: Bioengineering Research Institute is built up in Nanjing.
High-density lipoprotein cholesterol (HDL-C) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Alanine aminotransferase (ALT) testing cassete, total protein (TP) testing cassete, albumin (ALB) testing cassete and ball egg
White (GLB) testing cassete is all purchased from Nanjing and builds up Bioengineering Research Institute.
Female C57 mouse 30, is purchased from Guangdong Province's medical experiment animal by SPF grades, weight 12-14g, age in days 28-30d
Center, credit number are SCXK (Guangdong) 2013-0002.
Rats and mice maintains feed: Guangdong Medical Lab Animal Center, credit number SCXK (Guangdong) 2013-0002.
High cholesterol diet formula high in fat is as shown in table 2.
The main component and energy of the high cholesterol diet high in fat of table 2 are constituted
Russule extract is prepared by experimental example 1 of the present invention.
2, method
The preparation of high fat diet obesity-induced mice model: animal subject is raised in the zoopery of Institute of Micro-biology, Guangdong Province
The heart, raising temperature and humidity: 23 ± 1 DEG C, 55 ± 10%, using 12h intermittent illumination round the clock；Receptacle condition remains steady
It is fixed, with the reliability of guarantee test result.Mouse ad lib drinking-water.It is primary that period is checked daily animal, does not find unhealthy
Animal, take whole healthy mices to be tested.50 C57 mouse are randomly divided into normal group, model after adaptive feeding 7d
Group, russule alcohol extracting group, russule water propose group and russule alkali carries group, and every group 10.Normal group is fed with normal diet, model group and is given
Medicine group is fed with high cholesterol diet high in fat, continuous to feed 2 months.
After modeling success, start to be administered, administration group gives russule water extract, russule alcohol extracting thing and russule alkali carries respectively
Take object stomach-filling, drug concentration 6mg/ml (180g extract is dissolved in 30ml water), 3mg/ pcs/day of dosage, dosage 0.5ml/ only/
It, successive administration 35 days, while normal group mouse continues to be fed with normal diet, administration group and model group mouse use common feeding instead
Material is fed.
3, result is observed
The appearance posture (hair, color, shape) and dynamic of daily observation animal simultaneously make respective record, measure the weight of animals every 7d
1 time.
Blood: after being discontinued for 24 hours, animal materials testing index.Acquire blood sample, pluck eyeball and take blood, blood plasma through 3000RPM,
Serum is collected in 10min centrifugation twice, and it is horizontal to measure T-CHO, TG, LDL-C, HDL-C, TP, ALB, GLB, ALT in serum.
Tissue: it plucks after eyeball takes blood, puts to death and dissect mouse, take mouse small intestine and liver organization, be fixed on 4% poly first
In aldehyde.Small intestine and liver organization are made into paraffin section, and small intestine is dyed using Hematoxylin-eosin (HE) method, liver organization
Hematoxylin-eosin (HE), masson, oil red O (ORS) is respectively adopted, carries out pathology section examination.
4, data processing
All data are indicated with mean ± standard deviation (x ± s).The comparison of mean uses one-way analysis of variance between multiple groups
(One-Way ANOVA), mean compares two-by-two between group, and LSD method is used when variance is neat；Dunnett ' s T3 is used when heterogeneity of variance
Method.It is completed by SPSS software, α=0.05.
The results are shown in Table 3 for the changes of weight of each group.
The variation of 3 mouse weight of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
It has been observed that the fur of the animal of administration group is more smoother than the fur of model group.
From table 3 it can be seen that the fur of the animal by treatment is more smoother than the fur of model group.At the end of experiment, just
Average weight significant difference (P < 0.05) between normal group and model group, average weight is about when starting for model group mouse
65.45g, average weight is 72.76g at the end of experiment, illustrates high fat diet obesity-induced mice model modeling success；With mould
Type group is compared, and 3 administration group average mice body weights are remarkably decreased (P < 0.001) (table 3).This shows: suitable to obesity mice stomach-filling
The russule extract of amount can significantly slow weight gain.The four items of blood lipid tests testing result of each group is as shown in table 4.
4 mice serum biochemical indicator (mmol/L) of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
From table 4, it can be seen that in model group total cholesterol, triglycerides and ldl-cholesterol ratio normally organize it is aobvious
It writes and increases (P < 0.01 or P < 0.001), high-density lipoprotein cholesterol significantly reduces (P < 0.001) than normal group；With model group
It compares, under triglycerides (T-CHO), total cholesterol (TG), low density lipoprotein cholesterol (LDL-C) are horizontal significantly in administration group
It drops (P < 0.05 or P < 0.01 or P < 0.001), the horizontal significant raising (P < 0.001) of high-density lipoprotein cholesterol (HDL-C).This
Show: total cholesterol in serum, triglycerides and low can be significantly reduced to the suitable russule extract of obesity mice stomach-filling
Density lipoprotein levels.
The results are shown in Table 5 for the liver functional testing of each group.
5 liver functional testing result of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
Serum alt and ALB/GLB can reflect liver function damage degree, when liver cell is damaged, ALB synthesis decline, and ball egg
White balance imbalance, causes ALB/GLB to decline.As can be seen from Table 5, compared with normal group, ALT is significant in model group mice serum
It increases (P < 0.05), shows that high fat diet causes hepatic injury modeling success；Compared with model group, in administration group ALT significantly reduce (P <
0.05), ALB and ALB/GLB significantly increases (P < 0.05).Show that russule extract can improve hepatic injury.
Small intestine HE coloring pathological section result as shown in Figure 1, it can be seen from figure 1 that normally group small intestine's villus it is long and
Completely, model group intestinal villus falls off seriously, illustrates that high fat diet can cause to damage to the enteron aisle of mouse, illustrates that model is success
's.Compared with model group, after drug-treated, administration group can alleviate damage, promote the reparation of enteron aisle.
Liver organization pathological section result is as shown in Fig. 2, figure it is seen that HE coloration result shows normal group mouse
Liver cell structure is normal, and model group mouse liver cell volume increases, and occurs many circles not of uniform size in hepatocyte cell matter
Blown-out shot pushes nucleus to side, locally there is inflammatory cell infiltration phenomenon；Compared with model group, administration group mouse liver cell rouge
Fat vacuole quantity significantly reduces, and inflammatory cell infiltration phenomenon is improved；Masson is dyed and ORS coloration result is shown respectively,
Compared with model group, administration group mouse liver cell structure is obviously improved, and tends to normal group.
Inventor also found that high fat diet obesity-induced mice model makes its intestinal microflora that significant changes occur,
Russule extract is added in high cholesterol diet high in fat and is clearly helpful for dominant microflora structure recovery in enteron aisle, and it is more to facilitate flora
Sample is effectively improved intestinal bacilli illness, repairs intestinal mucosa, effectively inhibits the deterioration for alleviating inflammation, improves immune-related
Every physiopathological index.It has been recognised by the inventors that russule extract is based on this so as to improve caused by high fat diet
Hepatic injury can be used in the various fields such as drug, health care product, have great economy and society value.
The above, preferable specific embodiment only of the invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (10)
1. purposes of the russule extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease.
2. purposes according to claim 1, which is characterized in that the high fat diet causes the hepatic injury related disease to include but not
It is limited to hepatic steatosis, nonalcoholic steatohepatitis, cirrhosis, hepatic failure.
3. the preparation of a kind of prevention and/or treatment hepatic injury related disease, which is characterized in that the preparation is extracted comprising russule
Object.
4. preparation according to claim 3, which is characterized in that the high fat diet causes the hepatic injury related disease to include but not
It is limited to hepatic steatosis, nonalcoholic steatohepatitis, cirrhosis, hepatic failure.
5. the drug of a kind of prevention and/or treatment hepatic injury related disease, which is characterized in that comprising russule extract and pharmaceutically
Acceptable carrier.
6. drug according to claim 5, which is characterized in that the high fat diet causes the hepatic injury related disease to include but not
It is limited to hepatic steatosis, nonalcoholic steatohepatitis, cirrhosis, hepatic failure.
7. drug according to claim 5, which is characterized in that the russule extract includes russule water extract, russule
Alcohol extracting thing, russule alkali extract.
8. a kind of preparation method of russule extract characterized by comprising by russule fructification crush after, successively with alcohol extracting,
Water mentions, NaOH buck extracts, and respectively obtains extraction solution, then concentrated, freeze-drying respectively, obtains russule alcohol extracting thing, red
Mushroom water extract, russule alkali extract.
9. preparation method according to claim 8 characterized by comprising after crushing russule fructification, successively use
95% ethyl alcohol extracts, water extracts, 1%-2%NaOH buck extracts, and respectively obtains extraction solution, then concentrated, freezing is done respectively
It is dry, obtain russule alcohol extracting thing, russule water extract, russule alkali extract.
10. preparation method according to claim 8 characterized by comprising after crushing russule fructification, using second
Alcohol reflux extracts, and obtains the first filter residue and alcohol extracting solution, and the first filter residue is used water refluxing extraction solution, obtains the second filter residue
And water extraction solution, then the second filter residue is used into 1%-2%NaOH buck refluxing extraction, obtain third filter residue and alkali carries take it is molten
Alcohol extracting solution, water extraction solution are obtained russule alcohol extracting thing, russule water extract by concentration, freeze-drying respectively by liquid；
Solution is taken to obtain russule alkali extract by concentration, alcohol precipitation, freeze-drying alkali carries.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839677A (en) * | 2019-12-20 | 2020-02-28 | 浦北和香食品厂 | Russule mooncake and production method thereof |
| CN112569336A (en) * | 2020-12-16 | 2021-03-30 | 广东省微生物研究所（广东省微生物分析检测中心） | Application of grifola frondosa glycoprotein in preparation of drugs or health foods for enhancing body immunity and preventing and/or treating tumor diseases |
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| CN112569336B (en) * | 2020-12-16 | 2023-08-11 | 广东省微生物研究所（广东省微生物分析检测中心） | Application of russula cinerea glycoprotein in preparation of medicines or health foods for enhancing organism immunity and preventing and/or treating tumor diseases |
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