CN109771454A - Purposes of the birch young pilose antler extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease - Google Patents
Purposes of the birch young pilose antler extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease Download PDFInfo
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Abstract
The present invention relates to a kind of fungi extracts to treat and/or prevent the purposes of purposes more particularly to birch young pilose antler extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease in the preparation that high fat diet causes hepatic injury related disease.Of the invention preparation method is simple, operating cost is low, Extraction solvent in preparation method of the invention can be with recycling and reusing, no organic reagent remains risk, while ensure that the natural quality of birch young pilose antler extract and environmentally friendly, belongs to green manufacture technology.Raw material extraction of the present invention is more complete, takes full advantage of raw material, raw material availability is also very high on the basis of guaranteeing extract effect.Birch young pilose antler extract of the invention can not only be clearly helpful for the reparation that high fat diet causes hepatic injury, but also significantly treatment and/or preventive effect are shown to disease relevant to hepatic injury, it can be used in the various fields such as drug, health care product, there is great economy and society value.
Description
Technical field
The present invention relates to a kind of fungi extracts in the system for treating and/or preventing high fat diet cause hepatic injury related disease
Purposes more particularly to birch young pilose antler extract in agent cause hepatic injury related disease in preparation treatment and/or prevention high fat diet
Purposes in preparation.
Background technique
Enteric microorganism group is known as " second set of genome of human body ", closely bound up with human health.The micro- life of human intestine
Species are up to 1000 kinds or more, and wherein Firmicutes and Bacteroidetes are dominant microflora, and the gene dosage in enteron aisle genome is
More than 100 times of mankind itself's genome.There is mutualism relationships by intestinal flora and host, and it is flat that dynamic is remain in enteron aisle
Weighing apparatus, when balance is broken, people is possible to suffer from various diseases.Existing research confirms: (1) intestinal flora is related to obesity,
There are intestinal microflora imbalance, Phylogenetic diversity of bacteria declines for obese people；The Bacteroidetes of obesity mice is few, and Firmicutes are more；
The enteric microorganism of obesity mice is transplanted on germfree mouse, will lead to the increase of the latter's fat mass.For fat people,
Long-term intake high fat diet, can cause the change of intestinal microflora, and unbalance intestinal flora can generate endotoxin and enter human body,
Multiple inflammatory factors are generated after being identified by immunocyte, body is caused to be in low grade inflammation state, generate metabolic disorder, so that from
The energy absorbed in food is easily converted to fat, causes obesity；(2) liver and enteron aisle are close phase on biological function
It closes, is interactional.Liver and intestinal flora are interacted by intestines-liver axis, and intestinal flora is close with liver diseases, and liver is suffered from
When sick, the structure and function of intestinal flora will affect, and can influence in turn liver function when intestinal bacilli illness, cirrhosis
The beneficial bacteriums quantity such as patient Bifidobacterium reduces, and the harmful bacterias quantity such as enterobacteria, enterococcus increases.
High fat diet can change intestinal microflora, reduce beneficial bacterium, and harmful bacteria increases, and destroy the flat of intestinal flora
Weighing apparatus, and then liver function is influenced, hepatic injury is caused, and increasing the problems such as with the development and dietary structure of China's living standard
Add, the disease incidence of the hepatic injury as caused by high fat diet obviously increases, and there is an urgent need to develop treat and/or prevent high in fat in preparation
Diet causes the preparation in terms of hepatic injury related disease.
Summary of the invention
Against the above deficiency, the present invention provides a kind of birch young pilose antler extract and causes in preparation treatment and/or prevention high fat diet
Purposes in the preparation of hepatic injury related disease, function is significant clear, can medicinalization, have practicability.
The present invention reaches above-mentioned purpose by following scheme:
In the first aspect of the present invention, a kind of birch young pilose antler extract is provided treating and/or prevent high fat diet and causes liver damage
Hurt the purposes in related disease.
In the second aspect of the present invention, a kind of birch young pilose antler extract is provided and is caused in preparation treatment and/or prevention high fat diet
Purposes in the preparation of hepatic injury related disease.
In the third aspect of the present invention, a kind of system treating and/or preventing high fat diet and cause hepatic injury related disease is provided
Agent, the preparation include birch young pilose antler extract.
In the fourth aspect of the present invention, a kind of medicine treating and/or preventing high fat diet and cause hepatic injury related disease is provided
Object includes birch young pilose antler extract and pharmaceutically acceptable carrier.
In the fifth aspect of the invention, a kind of preparation method of birch young pilose antler extract is provided, comprising: by birch young pilose antler fructification
It after crushing, is successively mentioned with alcohol extracting, water, the extraction of sodium hydroxide (1%-2%NaOH aqueous solution) buck, respectively obtains extraction solution,
Concentrated, freeze-drying respectively again, obtains birch young pilose antler alcohol extracting thing, birch young pilose antler water extract, birch young pilose antler alkali extract.
Compared with prior art, the invention has the following beneficial effects:
(1) of the invention preparation method is simple, operating cost is low, and the Extraction solvent in preparation method of the invention is only
Ethyl alcohol, water, NaOH aqueous solution have been used, risk can have been remained with recycling and reusing, no organic reagent, ensure that birch young pilose antler extract
Natural quality while and it is environmentally friendly, belong to green manufacture technology.
(2) present invention successively uses ethyl alcohol, water, NaOH extraction with aqueous solution birch young pilose antler fructification, and raw material extraction is more complete, fills
Divide and raw material is utilized, raw material availability is also very high on the basis of guaranteeing extract effect.
(3) birch young pilose antler extract of the invention can not only be clearly helpful for the reparation that high fat diet causes hepatic injury, but also
Significantly treatment and/or preventive effect are shown to disease relevant to hepatic injury, such as the deterioration of inflammation can be effectively relieved, is changed
Be apt to immune-related every physiopathological index, can be used in the various fields such as drug, health care product, have it is greatly economical and
Social value.
Detailed description of the invention
Fig. 1 is the small intestine HE coloring pathological section figure of embodiment 2.
Fig. 2 is the liver organization pathological section figure of embodiment 2.
Specific embodiment
In the first aspect of the present invention, a kind of birch young pilose antler extract is provided treating and/or prevent high fat diet and causes liver damage
Hurt the purposes in related disease.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the second aspect of the present invention, a kind of birch young pilose antler extract is provided and is caused in preparation treatment and/or prevention high fat diet
Purposes in the preparation of hepatic injury related disease.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the third aspect of the present invention, the preparation of a kind of prevention and/or treatment hepatic injury related disease, the preparation includes
Birch young pilose antler extract.
Preferably, the preparation includes drug, health care product or food.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
In the fourth aspect of the present invention, a kind of drug prevented and/or treat hepatic injury related disease is mentioned comprising birch young pilose antler
Take object and pharmaceutically acceptable carrier.
Wherein, it includes but is not limited to hepatic steatosis, non-alcoholic rouge that the high fat diet, which causes hepatic injury related disease,
Fat hepatitis, cirrhosis, hepatic failure.
Heretofore described " pharmaceutically acceptable carrier " is being cured it is also understood that for " diluent " or " auxiliary material " etc.
It is known that and being described in such as Remington medical science (Remington ' s Pharmaceutical in medicine field
Sciences), Mack Publishing Company (Mack Publishing Co.) (AR lattice nano (A.R.Gennaro) compile,
1985).These substances are nontoxic for recipient under used dosage and concentration, including such as phosphate, citric acid
The buffers such as salt, acetate and other acylates；The antioxidants such as ascorbic acid；The low molecular weights such as poly arginine
(being less than about 10 residues) peptide；The protein such as seralbumin, gelatin or immunoglobulin；Such as polyvinylpyrrolidone
Equal hydrophilic polymers；The amino acid such as glycine, glutamic acid, aspartic acid or arginine；Monosaccharide, disaccharides and including cellulose
Or derivatives thereof, other carbohydrate of glucose, mannose or dextrin；The chelating agents such as EDTA；Such as mannitol or
The sugar alcohols such as sorbierite；The counter ion counterionsl gegenions such as sodium；And/or such as tween (Tween), Pluronic (Pluronics) or poly- second
The nonionic surface active agent such as glycol；Further including includes sterile water, salt water, glucose, oil (such as corn oil, peanut oil and class
Like object), acid.Further can also include preservative, wetting agent, emulsifier, and the like etc..
The dosage of birch young pilose antler extract or drug of the invention can be with specific medication and treated object
Or type and change.The dosage is enough to generate expected beneficial effect, and can be formulated for single dose application or multiple
Dosage application.
Heretofore described drug can be applied by any approach well known by persons skilled in the art, including oral,
The approach such as intramuscular, intravenous, intradermal, intralesional.Application can be part, surface or whole body.In any given feelings
Most suitable approach depends on many factors, such as property, the process of disease, the seriousness of disease of disease etc. under condition.As
It is preferred that birch young pilose antler extract of the invention or being administered orally comprising the drug including birch young pilose antler extract.
The birch young pilose antler extract of the invention can also be applied together with the second reactive compound/second therapeutic agent, together
When, in succession or parallel application.When applying respectively, the birch young pilose antler extract of the invention or the medicine comprising birch young pilose antler extract
Object can be applied from the second reactive compound or second therapeutic agent with different medicine frequencies or interval.
In the purposes of aforementioned four aspect of the invention or preparation, the birch young pilose antler extract includes that birch young pilose antler water extracts
Object, birch young pilose antler alcohol extracting thing, birch young pilose antler alkali extract.
In one embodiment, the birch young pilose antler alcohol extracting thing is birch young pilose antler ethanol extract.
In one embodiment, the birch young pilose antler alkali extract is birch young pilose antler sodium hydroxide extract.
Preferably, in the purposes of aforementioned four aspect of the invention or preparation, under the birch young pilose antler extract is preferably
State the birch young pilose antler extract that preparation method is prepared.
In the fifth aspect of the invention, a kind of preparation method of birch young pilose antler extract is provided, comprising: by birch young pilose antler fructification
It after crushing, is successively mentioned with alcohol extracting, water, the extraction of NaOH buck, respectively obtains extraction solution, then concentrated, freeze-drying respectively, obtained
To birch young pilose antler alcohol extracting thing, birch young pilose antler water extract, birch young pilose antler alkali extract.
Preferably, a kind of preparation method of birch young pilose antler extract, comprising: after crushing birch young pilose antler fructification, successively use
95% ethyl alcohol extracts, water extracts, 1%-2%NaOH buck extracts, and respectively obtains extraction solution, then concentrated, freezing is done respectively
It is dry, obtain birch young pilose antler alcohol extracting thing, birch young pilose antler water extract, birch young pilose antler alkali extract.
Preferably, a kind of preparation method of birch young pilose antler extract, comprising: after crushing birch young pilose antler fructification, using ethyl alcohol
Refluxing extraction obtains the first filter residue and alcohol extracting solution, by the first filter residue use water refluxing extraction solution, obtain the second filter residue and
Water extraction solution, then the second filter residue is used into 1%-2%NaOH buck refluxing extraction, it obtains third filter residue and alkali carries takes solution,
Alcohol extracting solution, water extraction solution are obtained into birch young pilose antler alcohol extracting thing by concentration, freeze-drying respectively, birch young pilose antler water extracts
Object；Solution is taken to obtain birch young pilose antler alkali extract by concentration, alcohol precipitation, freeze-drying alkali carries.
Preferably, it includes: that the ethyl alcohol that solid-liquid ratio is 1:8-1:15 is added that the alcohol reflux, which extracts, and refluxing extraction 2-4 is small
When, it filters, obtains filtrate, is concentrated under reduced pressure to give thick paste, -80 DEG C of freeze overnights of thick paste warp are freeze-dried to get birch young pilose antler alcohol extracting
Object.
Preferably, the water refluxing extraction includes: the filter residue after taking alcohol extracting, uses solid-liquid ratio for the water of 1:8-1:15, is returned
Stream extracts 1-3 hours, filters, obtains filtrate, is concentrated under reduced pressure to give thick paste, -80 DEG C of freeze overnights of thick paste warp, freeze-drying, i.e.,
Obtain birch young pilose antler water extract.
Preferably, the 1%-2%NaOH buck extract include: learn from else's experience alcohol and water mention after filter residue, use solid-liquid ratio for
The buck of 1:8-1:15, stirring is ultrasonic extraction 1-2 hours lower, filters, and obtains filtrate, and alcohol reagent precipitating is added, precipitates 12-24
Hour, precipitating is collected, through -80 DEG C of freeze overnights, is freeze-dried to get birch young pilose antler alkali carries object.
The 1%-2%NaOH or the 1%-2%NaOH buck refer to that weight percentage is the NaOH water of 1%-2%
Solution.
Preferably, the 1%-2%NaOH is 2%NaOH.
It is further preferred that the alcohol reagent of the precipitating includes ethyl alcohol, methanol.
It is further preferred that described be precipitated as that 4-6 times of the liquid ethyl alcohol measured is added, precipitate 12-24 hours.
In the sixth aspect of the present invention, a kind of birch young pilose antler extract that above-mentioned preparation method is prepared is provided.
Birch is fine and soft: [Inonotuso bliquus (Fr) Pilat] its sclerotium is medicinal fungi, also known as: Inonotus obliquus, white
Birch young pilose antler, glossy ganoderma in siberia, birch mushroom.Warty, sclerotium stockless, general diameter 25cm~40cm, appearance is presented in birch young pilose antler sclerotium
Dark brown or black, internal yellowish-brown, old rear surface drastic crack are very firmly, crisp when doing.It is living to be born in white birch, silvery birch, elm, alder etc.
Under the bark of standing tree or felling after trees it is dried-up on, sclerotium can after felling it is dried-up it is upper existence for 6 years.Birch
Fine and soft mycelium is extremely cold-resistant, and mycelium in wood of living is resistant to subzero 40 DEG C of low temperature.It is mainly distributed on Northern Hemisphere north
The area that 45 °~50 ° of latitude, as (West Siberia, Far East some areas, prospecting add for North America (the north), Finland, Poland, Russia
The peninsula), Chinese (Heilungkiang, Jilin Province Changbaishan area), the countries such as Japanese (Hokkaido).Birch young pilose antler contain a large amount of anticancer,
Blood pressure lowering, hypoglycemic, reducing blood lipid, the plant fiber class polysaccharide body for bringing back to life immunization.The vigor of immunocyte can be improved, press down
Cancer cell diffusion and recurrence processed, prevent the absorption of the harmful substances such as carcinogen, and promote to drain in stomach and intestine.
Heretofore described " imbalance " can be understood as " unbalance ", " disorder " or " disease " etc..
As used herein, term "comprising" or " comprising " refer to that method includes cited element, but are not excluded for other
Element.
The chemical component of birch young pilose antler birch young pilose antler of the present invention has polysaccharide, inonotus obliquus, Inonotus obliquus alcohol, a variety of oxygen
Change triterpene compound, bolt bacterium acid, a variety of lanosterol type triterpene compounds, folic acid derivatives, aromatic vanillic acid, fourth
Fragrant acid and γ hydroxybenzoic acid, also it has been reported that separated tannin compound, steroids, alkaloid compound, melanin out
Class, low molecule Polyphenols and lignin compound.
Below in conjunction with specific embodiment, invention is further explained.
Embodiment 1: the preparation of birch young pilose antler extract, steps are as follows:
(1) birch young pilose antler fructification is crushed, uses solid-liquid ratio for the ethyl alcohol of 1:8-1:15, refluxing extraction 2-4 hours, taken out
Filter, the thick paste for obtaining filtrate, being concentrated under reduced pressure to give debita spissitudo, -80 DEG C of freeze overnights of thick paste warp enter freeze drier drying,
Up to birch young pilose antler alcohol extracting thing.
(2) the above-mentioned filter residue after alcohol extracting is taken, uses solid-liquid ratio for the water of 1:8-1:15, refluxing extraction 1-3 hours, is taken out
Filter, the thick paste for obtaining filtrate, being concentrated under reduced pressure to give debita spissitudo, -80 DEG C of freeze overnights of thick paste warp enter freeze drier drying,
Up to birch young pilose antler water extract.
(3) the above-mentioned filter residue after alcohol and water mentions is taken, uses solid-liquid ratio for the 2%NaOH aqueous solution of 1:8-1:15, is stirred
Lower ultrasonic extraction 1-2 hours filters, and obtains filtrate, and debita spissitudo ethyl alcohol is added, and precipitates 12-24 hours；Precipitating is collected, through -80
It is dry to get birch young pilose antler buck extract to enter freeze drier for DEG C freeze overnight.
Obtained birch young pilose antler alcohol extracting thing, birch young pilose antler water extract, birch young pilose antler buck extract are subjected to polyoses content survey
Fixed, using People's Republic of China's agricultural industry criteria NY/T 1676-2008, " Thick many candies contain determination of polysaccharide in edible mushroom
The measurement of amount " method；Protein content determination uses Kjeldahl's method.
Table 1 is the Thick many candies content measured in birch young pilose antler extract and protein content.
Thick many candies and protein content in 1 birch young pilose antler extract of table
Embodiment 2: improvement result of the birch young pilose antler extract to high fat diet obesity-induced mice hepatic injury
1, reagent:
Total cholesterol (T-CHO) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Triglycerides (TG) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Low density lipoprotein cholesterol (LDL-C) testing cassete: Bioengineering Research Institute is built up in Nanjing.
High-density lipoprotein cholesterol (HDL-C) testing cassete: Bioengineering Research Institute is built up in Nanjing.
Alanine aminotransferase (ALT) testing cassete, total protein (TP) testing cassete, albumin (ALB) testing cassete and ball egg
White (GLB) testing cassete is all purchased from Nanjing and builds up Bioengineering Research Institute.
Female C57 mouse 30, is purchased from Guangdong Province's medical experiment animal by SPF grades, weight 12-14g, age in days 28-30d
Center, credit number are SCXK (Guangdong) 2013-0002.
Rats and mice maintains feed: Guangdong Medical Lab Animal Center, credit number SCXK (Guangdong) 2013-0002.
High cholesterol diet formula high in fat is as shown in table 2.
The main component and energy of the high cholesterol diet high in fat of table 2 are constituted
Birch young pilose antler extract is prepared by experimental example 1 of the present invention.
2, method
The preparation of high fat diet obesity-induced mice model: animal subject is raised in the zoopery of Institute of Micro-biology, Guangdong Province
The heart, raising temperature and humidity: 23 ± 1 DEG C, 55 ± 10%, using 12h intermittent illumination round the clock；Receptacle condition remains steady
It is fixed, with the reliability of guarantee test result.Mouse ad lib drinking-water.It is primary that period is checked daily animal, does not find unhealthy
Animal, take whole healthy mices to be tested.50 C57 mouse are randomly divided into normal group, model after adaptive feeding 7d
Group, birch young pilose antler alcohol extracting group, birch young pilose antler water propose group and birch young pilose antler alkali carries group, and every group 10.Normal group is fed with normal diet, model
Group and administration group are fed with high cholesterol diet high in fat, continuous to feed 2 months.
After modeling success, start to be administered, administration group gives birch young pilose antler water extract, birch young pilose antler alcohol extracting thing and birch respectively
Fine and soft alkali extract stomach-filling, drug concentration 6mg/ml (180g extract is dissolved in 30ml water), 3mg/ pcs/day of dosage, dosage
0.5ml/ pcs/day, successive administration 35 days, while normal group mouse continues to be fed with normal diet, administration group and model group mouse
Use normal diet nursing instead.
3, result is observed
The appearance posture (hair, color, shape) and dynamic of daily observation animal simultaneously make respective record, measure the weight of animals every 7d
1 time.
Blood: after being discontinued for 24 hours, animal materials testing index.Acquire blood sample, pluck eyeball and take blood, blood plasma through 3000RPM,
Serum is collected in 10min centrifugation twice, and it is horizontal to measure T-CHO, TG, LDL-C, HDL-C, TP, ALB, GLB, ALT in serum.
Tissue: it plucks after eyeball takes blood, puts to death and dissect mouse, take mouse small intestine and liver organization, be fixed on 4% poly first
In aldehyde.Small intestine and liver organization are made into paraffin section, and small intestine is dyed using Hematoxylin-eosin (HE) method, liver organization
Hematoxylin-eosin (HE), masson, oil red O (ORS) is respectively adopted, carries out pathology section examination.
4, data processing
All data are with mean ± standard deviationIt indicates.The comparison of mean uses one-way analysis of variance between multiple groups
(One-Way ANOVA), mean compares two-by-two between group, and LSD method is used when variance is neat；Dunnett ' s T3 is used when heterogeneity of variance
Method.It is completed by SPSS software, α=0.05.
The changes of weight of each group is as shown in table 3.
The variation of 3 mouse weight of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
It has been observed that the fur of the animal of administration group is more smoother than the fur of model group.
From table 3 it can be seen that the fur of the animal by treatment is more smoother than the fur of model group.At the end of experiment, just
Average weight significant difference (P < 0.05) between normal group and model group, average weight is about when starting for model group mouse
65.45g, average weight is 72.76g at the end of experiment, illustrates high fat diet obesity-induced mice model modeling success；With mould
Type group is compared, and 3 administration group average mice body weights are remarkably decreased (P < 0.001) (table 3).This shows: suitable to obesity mice stomach-filling
The birch young pilose antler extract of amount can significantly slow weight gain.
The four items of blood lipid tests testing result of each group is as shown in table 4.
4 mice serum biochemical indicator (mmol/L) of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
From table 4, it can be seen that in model group total cholesterol, triglycerides and ldl-cholesterol ratio normally organize it is aobvious
It writes and increases (P < 0.01 or P < 0.001), high-density lipoprotein cholesterol significantly reduces (P < 0.001) than normal group；With model group
It compares, triglycerides, total cholesterol, low-density lipoprotein cholesterol level are remarkably decreased (P < 0.05 or P < 0.01 in administration group
Or P < 0.001), High-density Lipoprotein-cholesterol significantly increases (P < 0.05).This shows: suitable to obesity mice stomach-filling
Total cholesterol, triglycerides and low-density lipoprotein white level in serum can be significantly reduced in birch young pilose antler extract.
The results are shown in Table 5 for the liver functional testing of each group.
5 liver functional testing result of table
#P < 0.05,##P < 0.01,###Normal group of P < 0.001vs；*P < 0.05,**P < 0.01,***P < 0.001vs model group
Serum alt and ALB/GLB can reflect liver function damage degree, when liver cell is damaged, ALB synthesis decline, and ball egg
White balance imbalance, causes ALB/GLB to decline.As can be seen from Table 5, compared with normal group, ALT is significant in model group mice serum
It increases (P < 0.05), shows that high fat diet causes hepatic injury modeling success；Compared with model group, in administration group ALT significantly reduce (P <
0.05), ALB and ALB/GLB significantly increases (P < 0.05).Show that birch young pilose antler extract can improve hepatic injury.
Small intestine HE coloring pathological section result as shown in Figure 1, it can be seen from figure 1 that normally group small intestine's villus it is long and
Completely, model group intestinal villus falls off seriously, illustrates that high fat diet can cause to damage to the enteron aisle of mouse, illustrates that model is success
's.Compared with model group, after drug-treated, administration group can alleviate damage, promote the reparation of enteron aisle.
Liver organization pathological section result is as shown in Fig. 2, figure it is seen that HE coloration result shows normal group mouse
Liver cell structure is normal, and model group mouse liver cell volume increases, and occurs many circles not of uniform size in hepatocyte cell matter
Blown-out shot pushes nucleus to side, locally there is inflammatory cell infiltration phenomenon；Compared with model group, administration group mouse liver cell rouge
Fat vacuole quantity significantly reduces, and inflammatory cell infiltration phenomenon is improved；Masson is dyed and ORS coloration result is shown respectively,
Compared with model group, administration group mouse liver cell structure is obviously improved, and tends to normal group.
Inventor also found that high fat diet obesity-induced mice model makes its intestinal microflora that significant changes occur,
Birch young pilose antler extract is added in high cholesterol diet high in fat and is clearly helpful for dominant microflora structure recovery in enteron aisle, facilitates flora
Diversification is effectively improved intestinal bacilli illness, repairs intestinal mucosa, effectively inhibits the deterioration for alleviating inflammation, improves immune correlation
Every physiopathological index.It has been recognised by the inventors that birch young pilose antler extract is based on this and leads so as to improve high fat diet
The hepatic injury of cause can be used in the various fields such as drug, health care product, have great economy and society value.
The above, preferable specific embodiment only of the invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (10)
1. purposes of the birch young pilose antler extract in the preparation that preparation treatment and/or prevention high fat diet cause hepatic injury related disease.
2. purposes according to claim 1, which is characterized in that the high fat diet causes the hepatic injury related disease to include but not
It is limited to hepatic steatosis, nonalcoholic steatohepatitis, cirrhosis, hepatic failure.
3. the preparation that a kind of prevention and/or treatment high fat diet cause hepatic injury related disease, which is characterized in that the preparation includes
Birch young pilose antler extract.
4. preparation according to claim 3, which is characterized in that the high fat diet causes the hepatic injury related disease to include but not
It is limited to hepatic steatosis, nonalcoholic steatohepatitis, cirrhosis, hepatic failure.
5. the drug that a kind of prevention and/or treatment high fat diet cause hepatic injury related disease, which is characterized in that mentioned comprising birch young pilose antler
Take object and pharmaceutically acceptable carrier.
6. drug according to claim 5, which is characterized in that the high fat diet causes the hepatic injury related disease to include but not
It is limited to hepatic steatosis, nonalcoholic steatohepatitis, cirrhosis, hepatic failure.
7. drug according to claim 5, which is characterized in that the birch young pilose antler extract include birch young pilose antler water extract,
Birch young pilose antler alcohol extracting thing, birch young pilose antler alkali extract.
8. a kind of preparation method of birch young pilose antler extract characterized by comprising after crushing birch young pilose antler fructification, successively use
Alcohol extracting, water mention, NaOH buck extracts, and respectively obtain extraction solution, then concentrated, freeze-drying respectively, obtain birch young pilose antler alcohol extracting
Take object, birch young pilose antler water extract, birch young pilose antler alkali extract.
9. preparation method according to claim 8 characterized by comprising after crushing birch young pilose antler fructification, successively use
95% ethyl alcohol extracts, water extracts, 1%-2%NaOH buck extracts, and respectively obtains extraction solution, then concentrated, freezing is done respectively
It is dry, obtain birch young pilose antler alcohol extracting thing, birch young pilose antler water extract, birch young pilose antler alkali extract.
10. preparation method according to claim 8 characterized by comprising after crushing birch young pilose antler fructification, use
Alcohol reflux extracts, and obtains the first filter residue and alcohol extracting solution, and the first filter residue is used water refluxing extraction solution, obtains the second filter
Slag and water extraction solution, then the second filter residue is used into 1%-2%NaOH refluxing extraction, it obtains third filter residue and alkali carries takes solution,
Alcohol extracting solution, water extraction solution are obtained into birch young pilose antler alcohol extracting thing by concentration, freeze-drying respectively, birch young pilose antler water extracts
Object；Solution is taken to obtain birch young pilose antler alkali extract by concentration, alcohol precipitation, freeze-drying alkali carries.
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| CN114794338A (en) * | 2022-05-07 | 2022-07-29 | 山东七河生物科技股份有限公司 | Preparation method of composite edible fungus healthy flavor beverage |
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