CN106108106A - A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh and application simultaneously - Google Patents
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh and application simultaneously Download PDFInfo
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- CN106108106A CN106108106A CN201610499141.1A CN201610499141A CN106108106A CN 106108106 A CN106108106 A CN 106108106A CN 201610499141 A CN201610499141 A CN 201610499141A CN 106108106 A CN106108106 A CN 106108106A
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- cloud
- tobaccos
- essential oil
- static pressure
- produces
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Classifications
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
Abstract
The present invention disclose a kind of cloud produce essential oil of tobaccos high static pressure extract the method with sterilization fresh simultaneously, comprise the following steps: 1) cloud produce Nicotiana tabacum L. tentatively pulverize；2) cloud produces the pulverizing of Nicotiana tabacum L. microjet；3) cloud produces the acquisition of tobacco extract；4) cloud produces the acquisition of essential oil of tobaccos；5) cloud produces the encapsulation of essential oil of tobaccos；6) cloud produces the high static pressure sterilization of essential oil of tobaccos；The high static pressure of this cloud product essential oil of tobaccos extracts the method with sterilization fresh simultaneously and has process process simply, non-environmental-pollution, technique is simple, and have efficiently, save time and low cost and other advantages, while retaining cloud product essential oil of tobaccos fragrance component, effectively extend the shelf-life of product.
Description
Technical field
The invention belongs to active skull cap components extractive technique field, the high static pressure being specifically related to a kind of cloud product essential oil of tobaccos is same
Time extract and the method for sterilization fresh and application.
Background technology
High static pressure (High Hydrostatic Pressure, HHP) refers to be placed in the material being sealed in flexible container
In pressure system, using water or other liquid as transmission medium, use the pressure treatment material of more than 100MPa, to reach to kill
Bacterium, enzyme denaturing and improve the purpose of material functional characteristic.At present, HHP sterilization technology is mainly used in the solid-state food containing liquid component
Product or the sterilization of food liquid, (" a kind of supertension in terms of the sterilization of beverage, milk, fruit and vegetable product, milk product, meat products etc.
The production method of homogenizing sterilization Lac Bovis seu Bubali ", the patent No.: 201310729089.0；" a kind of sterilization fresh method of wolfberry juice and industry
Change production method ", the patent No.: 201510133890.8；" a kind of Fructus Mori jam super-high-pressure sterilizing method ", the patent No.:
201410664252.4；" a kind of Tea flower essence, its preparation method and the application in Medicated cigarette thereof ", the patent No.:
201310446881.5) application that, it but prepare essential oil of tobaccos and sterilization simultaneously is less.In HHP sterilization process, due to
Some material component and structural state are sufficiently complex, therefore to take different treatment conditions according to different material objects.One
As affect HHP sterilization principal element have: pressure size, pressing time, pressed temperature etc..This experiment is attempted producing Nicotiana tabacum L. with cloud
Sample, research HHP technology is prepared the application extracted in terms of cloud produces essential oil of tobaccos and sterilization at the same time, is inquired into the antibacterial technology of non-heating power
Application in essential oil of tobaccos produces and stores.
Current domestic cloud produces the extracting method of essential oil of tobaccos mainly traditional steam distillation, organic solvent extractionprocess
Deng, traditional method generally also exists respective shortcoming, as low in the response rate, impurity is many, dissolvent residual and high temperature cause thermal sensitivity to become
Destruction divided etc..
Summary of the invention
It is an object of the invention to overcome the weak point of existing extraction and high-temperature sterilization technique, it is provided that a kind of cloud produces Nicotiana tabacum L. essence
The high static pressure of oil extracts the method with sterilization fresh simultaneously；Obtain cloud produce essential oil of tobaccos have raising cigarette flavor organoleptic quality and
The advantage increasing fragrance component concentration, and apply in tobacco product；The yield of this method target component is high, and extraction efficiency is high；Former
Expecting to extract and cold sterilization through too high static pressure, process processes simple, non-environmental-pollution, and technique is simple, and have efficiently, save time and
Low cost and other advantages, while retaining cloud product essential oil of tobaccos fragrance component, effectively extends the shelf-life of product, and the method is
The cloud that tobacco business provides a kind of environmental protection, have a extensive future produces the technology that essential oil of tobaccos extracts and improves preservation term.
Technology and method in order to solve the employing of the above-mentioned problem present invention are as follows:
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh simultaneously, comprises the following steps:
1) cloud product Nicotiana tabacum L. is tentatively pulverized: takes after cloud produces Nicotiana tabacum L. cleaning and dries, is tentatively crushed to 200 with super micron mill
After mesh, sieve and standby；
2) cloud produce Nicotiana tabacum L. microjet pulverize: take step 1) gained 200 mesh clouds produce Nicotiana tabacum L. ultra-micro powder samples, add 60%
Ethanol water soaks, and processes also circular treatment 20 times with 100MPa high shear microjet pulverizer after uniform stirring；
3) cloud produces the acquisition of tobacco extract: by step 2) after the soak of gained extracts under high static pressure pressure,
Centrifugal 10min under 10000r/min, obtains cloud and produces tobacco extract；
4) cloud produces the acquisition of essential oil of tobaccos: by step 3) cloud that obtains produces and adds β-N-acetyl glucosaminidase in tobacco extract
Enzyme, regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation is extracted cloud and produced the perfume (or spice) in Nicotiana tabacum L.
Gas composition, and reclaim fragrance component by liquid nitrogen cold trap absorption, after cooling, carry out breakdown of emulsion with NaCl, then with petroleum ether extraction,
Dry up with nitrogen and after petroleum ether removed by instrument, obtain cloud product essential oil of tobaccos；
5) cloud produces the encapsulation of essential oil of tobaccos: by 4) cloud that obtains produces the polyethylene high temperature steaming of essential oil of tobaccos high-temperature sterilization
Boil bag vacuum to seal, gnotobasis carries out double casing；
6) cloud produce essential oil of tobaccos high static pressure sterilization: immediately to 5) obtain be packaged with cloud produce essential oil of tobaccos packaging bag put
Enter in the pressure chamber of high static pressure processing means, and be immersed in pure water transmission medium process, the cloud then handled well by high static pressure
Producing essential oil of tobaccos sample and carry out microorganism detection according to the detection method of national standard after 24h, testing result is simultaneously with CFU/g
Or the logarithm that the microorganism of CFU/mL and Weibull model calculating reduces represents, if time conditions cannot meet, then by sample
It is placed on stored refrigerated in the refrigerator of 4 DEG C.
Further, in step 2) in, cloud produces Nicotiana tabacum L. ultra-micro powder sample and adds according to the solid-to-liquid ratio of 1:40～1:60
60% ethanol water soaks.
Further, in step 3) in, soak extracts under the high static pressure pressure of 100～300MPa.
Further, in step 3) in, soak extracts 30～60min under high static pressure pressure.
Further, in step 4) in, the consumption of β-N-acetyl glucosaminidase enzyme is 0.5%～1.5%.
Further, in step 6) in, the high static pressure sterilization pressure of high static pressure processing means is 400-600MPa.
Further, in step 6) in, the high static pressure sterilization temperature of high static pressure processing means is 30-50 DEG C.
Further, in step 6) in, the high static pressure sterilizing time of high static pressure processing means is 20-40min.
The invention have the benefit that the cloud prepared produces essential oil of tobaccos and has raising cigarette flavor organoleptic quality and increasing
Adding the effect of fragrance constituent concentration, and be suitable for industrialized production, gained cloud produces essential oil of tobaccos yield and extracts than vapor distillation
Method can improve 1.77-2.64 times, can improve 1.55-2.31 times than conventional petroleum ether extraction method；Gained improves tobacco aroma sense organ
Quality effect is higher than vapor distillation extraction method improves 38.27%, higher than traditional ether soxhlet extraction improves
42.58%；Gained improves that aroma components total concentration is higher than vapor distillation extraction method improves 30.12%, than tradition
Ether soxhlet extraction is the highest improves 24.95%, has the effect and significantly significantly improving tobacco aroma organoleptic quality
Reduce the effect of product preservation term total plate count.
Accompanying drawing explanation
Fig. 1 is each embodiment and relative value's comparison diagram of existing extraction method in the present invention；
Fig. 2 is each embodiment and existing routine thermally-sterilized preservation term total plate count comparison diagram in the present invention.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but they are not to skill of the present invention
The restriction of art scheme, based on present invention teach that any conversion made, all falls within protection scope of the present invention.
Embodiment 1
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh simultaneously, and step is as follows:
1) cloud product Nicotiana tabacum L. is tentatively pulverized: the cloud dried after weighing a certain amount of cleaning produces Nicotiana tabacum L., at the beginning of carrying out with super micron mill
After step is crushed to 200 mesh, sieve and standby；
2) cloud produces the pulverizing of Nicotiana tabacum L. microjet: the 200 mesh clouds taking certain step (1) gained produce Nicotiana tabacum L. ultra-micro powder samples, press
It is that 1:40 adds 60% ethanol water immersion according to solid-to-liquid ratio, processes with 100MPa high shear microjet pulverizer after uniform stirring
And circular treatment 20 times；
3) cloud produce tobacco extract acquisition: by the soak height static pressure pressure of step (2) gained be under 100MPa extract
After 30min, centrifugal 10min under 10000rpm, obtain cloud and produce tobacco extract；
4) cloud produces the acquisition of essential oil of tobaccos: cloud step (3) obtained produces in tobacco extract and is added into 0.5% β-N-acetyl
Glucosidase, regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation extracts Yun Chanyan
Fragrance component in grass, and reclaim fragrance component by liquid nitrogen cold trap absorption；After cooling, carry out breakdown of emulsion with NaCl, then with stone
Oil ether extraction, dries up with nitrogen and obtains cloud product essential oil of tobaccos after petroleum ether removed by instrument；
5) cloud produces the encapsulation of essential oil of tobaccos: the cloud (4) obtained produces the polyethylene high temperature of essential oil of tobaccos high-temperature sterilization
Retort pouch vacuum seals, and carries out double casing in gnotobasis；
6) cloud produces the high static pressure sterilization of essential oil of tobaccos: the cloud that is packaged with obtained (5) immediately produces the packaging bag of essential oil of tobaccos
Put into the pressure chamber of high static pressure processing means, and be immersed in pure water transmission medium；Controlling high static pressure sterilization pressure is
400MPa, high static pressure sterilization temperature are 30 DEG C and high static pressure sterilizing time is 20min；Then the Yun Chanyan high static pressure handled well
Grass essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull model calculate microorganism reduce logarithm represent；If time conditions cannot meet, sample should be put
Put stored refrigerated in the refrigerator of 4 DEG C.
The yield measuring gained cloud product essential oil of tobaccos is 3.12%.
Embodiment 2
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh simultaneously, and embodiment step is as follows:
1) cloud product Nicotiana tabacum L. is tentatively pulverized: the cloud dried after weighing a certain amount of cleaning produces Nicotiana tabacum L., at the beginning of carrying out with super micron mill
After step is crushed to 200 mesh, sieve and standby；
2) cloud produces the pulverizing of Nicotiana tabacum L. microjet: the 200 mesh clouds taking certain step (1) gained produce Nicotiana tabacum L. ultra-micro powder samples, press
It is that 1:50 adds 60% ethanol water immersion according to solid-to-liquid ratio, processes with 100MPa high shear microjet pulverizer after uniform stirring
And circular treatment 20 times；
3) cloud produce tobacco extract acquisition: by the soak height static pressure pressure of step (2) gained be under 200MPa extract
After 50min, centrifugal 10min under 10000rpm, obtain cloud and produce tobacco extract；
4) cloud produces the acquisition of essential oil of tobaccos: cloud step (3) obtained produces in tobacco extract and is added into 1.0% β-N-acetyl
Glucosidase, regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation extracts Yun Chanyan
Fragrance component in grass, and reclaim fragrance component by liquid nitrogen cold trap absorption；After cooling, carry out breakdown of emulsion with NaCl, then with stone
Oil ether extraction, dries up with nitrogen and obtains cloud product essential oil of tobaccos after petroleum ether removed by instrument；
5) cloud produces the encapsulation of essential oil of tobaccos: the cloud (4) obtained produces the polyethylene high temperature of essential oil of tobaccos high-temperature sterilization
Retort pouch vacuum seals, and carries out double casing in gnotobasis；
6) cloud produces the high static pressure sterilization of essential oil of tobaccos: the cloud that is packaged with obtained (5) immediately produces the packaging bag of essential oil of tobaccos
Put into the pressure chamber of high static pressure processing means, and be immersed in pure water transmission medium；Controlling high static pressure sterilization pressure is
500MPa, high static pressure sterilization temperature are 40 DEG C and high static pressure sterilizing time is 30min；Then the Yun Chanyan high static pressure handled well
Grass essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull model calculate microorganism reduce logarithm represent；If time conditions cannot meet, sample should be put
Put stored refrigerated in the refrigerator of 4 DEG C.
The yield measuring gained cloud product essential oil of tobaccos is 3.17%.
Embodiment 3
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh simultaneously, and embodiment step is as follows:
1) cloud product Nicotiana tabacum L. is tentatively pulverized: the cloud dried after weighing a certain amount of cleaning produces Nicotiana tabacum L., at the beginning of carrying out with super micron mill
After step is crushed to 200 mesh, sieve and standby；
2) cloud produces the pulverizing of Nicotiana tabacum L. microjet: the 200 mesh clouds taking certain step (1) gained produce Nicotiana tabacum L. ultra-micro powder samples, press
It is that 1:60 adds 60% ethanol water immersion according to solid-to-liquid ratio, processes with 100MPa high shear microjet pulverizer after uniform stirring
And circular treatment 20 times；
3) cloud produce tobacco extract acquisition: by the soak height static pressure pressure of step (2) gained be under 300MPa extract
After 30min, centrifugal 10min under 10000rpm, obtain cloud and produce tobacco extract；
4) cloud produces the acquisition of essential oil of tobaccos: cloud step (3) obtained produces in tobacco extract and is added into 0.5% β-N-acetyl
Glucosidase, regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation extracts Yun Chanyan
Fragrance component in grass, and reclaim fragrance component by liquid nitrogen cold trap absorption；After cooling, carry out breakdown of emulsion with NaCl, then with stone
Oil ether extraction, dries up with nitrogen and obtains cloud product essential oil of tobaccos after petroleum ether removed by instrument；
5) cloud produces the encapsulation of essential oil of tobaccos: the cloud (4) obtained produces the polyethylene high temperature of essential oil of tobaccos high-temperature sterilization
Retort pouch vacuum seals, and carries out double casing in gnotobasis；
6) cloud produces the high static pressure sterilization of essential oil of tobaccos: the cloud that is packaged with obtained (5) immediately produces the packaging bag of essential oil of tobaccos
Put into the pressure chamber of high static pressure processing means, and be immersed in pure water transmission medium；Controlling high static pressure sterilization pressure is
600MPa, high static pressure sterilization temperature are 50 DEG C and high static pressure sterilizing time is 20min；Then the Yun Chanyan high static pressure handled well
Grass essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull model calculate microorganism reduce logarithm represent；If time conditions cannot meet, sample should be put
Put stored refrigerated in the refrigerator of 4 DEG C.
The yield measuring gained cloud product essential oil of tobaccos is 2.67%.
Embodiment 4
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh simultaneously, and embodiment step is as follows:
(1) cloud product Nicotiana tabacum L. is tentatively pulverized: the cloud dried after weighing a certain amount of cleaning produces Nicotiana tabacum L., at the beginning of carrying out with super micron mill
After step is crushed to 200 mesh, sieve and standby；
(2) cloud produces the pulverizing of Nicotiana tabacum L. microjet: the 200 mesh clouds taking certain step (1) gained produce Nicotiana tabacum L. ultra-micro powder samples, press
It is that 1:40 adds 60% ethanol water immersion according to solid-to-liquid ratio, processes with 100MPa high shear microjet pulverizer after uniform stirring
And circular treatment 20 times；
(3) cloud produce tobacco extract acquisition: by the soak height static pressure pressure of step (2) gained be under 200MPa extract
After 60min, centrifugal 10min under 10000rpm, obtain cloud and produce tobacco extract；
(4) cloud produces the acquisition of essential oil of tobaccos: cloud step (3) obtained produces in tobacco extract and is added into 1.0% β-N-second
Acyl glucosidase, regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation is extracted cloud and produced
Fragrance component in Nicotiana tabacum L., and reclaim fragrance component by liquid nitrogen cold trap absorption；After cooling, carry out breakdown of emulsion with NaCl, then with
Petroleum ether extraction, dries up with nitrogen and obtains cloud product essential oil of tobaccos after petroleum ether removed by instrument；
(5) cloud produces the encapsulation of essential oil of tobaccos: the cloud (4) obtained produces the polyethylene high temperature of essential oil of tobaccos high-temperature sterilization
Retort pouch vacuum seals, and carries out double casing in gnotobasis；
(6) cloud produces the high static pressure sterilization of essential oil of tobaccos: the cloud that is packaged with obtained (5) immediately produces the packaging bag of essential oil of tobaccos
Put into the pressure chamber of high static pressure processing means, and be immersed in pure water transmission medium；Controlling high static pressure sterilization pressure is
600MPa, high static pressure sterilization temperature are 30 DEG C and high static pressure sterilizing time is 30min；Then the Yun Chanyan high static pressure handled well
Grass essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull model calculate microorganism reduce logarithm represent；If time conditions cannot meet, sample should be put
Put stored refrigerated in the refrigerator of 4 DEG C.
The yield measuring gained cloud product essential oil of tobaccos is 2.45%.
Embodiment 5
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh simultaneously, and embodiment step is as follows:
(1) cloud product Nicotiana tabacum L. is tentatively pulverized: the cloud dried after weighing a certain amount of cleaning produces Nicotiana tabacum L., at the beginning of carrying out with super micron mill
After step is crushed to 200 mesh, sieve and standby；
(2) cloud produces the pulverizing of Nicotiana tabacum L. microjet: the 200 mesh clouds taking certain step (1) gained produce Nicotiana tabacum L. ultra-micro powder samples, press
It is that 1:50 adds 60% ethanol water immersion according to solid-to-liquid ratio, processes with 100MPa high shear microjet pulverizer after uniform stirring
And circular treatment 20 times；
(3) cloud produce tobacco extract acquisition: by the soak height static pressure pressure of step (2) gained be under 300MPa extract
After 40min, centrifugal 10min under 10000rpm, obtain cloud and produce tobacco extract；
(4) cloud produces the acquisition of essential oil of tobaccos: cloud step (3) obtained produces in tobacco extract and is added into 1.5% β-N-second
Acyl glucosidase, regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation is extracted cloud and produced
Fragrance component in Nicotiana tabacum L., and reclaim fragrance component by liquid nitrogen cold trap absorption；After cooling, carry out breakdown of emulsion with NaCl, then with
Petroleum ether extraction, dries up with nitrogen and obtains cloud product essential oil of tobaccos after petroleum ether removed by instrument；
(5) cloud produces the encapsulation of essential oil of tobaccos: the cloud (4) obtained produces the polyethylene high temperature of essential oil of tobaccos high-temperature sterilization
Retort pouch vacuum seals, and carries out double casing in gnotobasis；
(6) cloud produces the high static pressure sterilization of essential oil of tobaccos: the cloud that is packaged with obtained (5) immediately produces the packaging bag of essential oil of tobaccos
Put into the pressure chamber of high static pressure processing means, and be immersed in pure water transmission medium；Controlling high static pressure sterilization pressure is
400MPa, high static pressure sterilization temperature are 40 DEG C and high static pressure sterilizing time is 20min；Then the Yun Chanyan high static pressure handled well
Grass essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull model calculate microorganism reduce logarithm represent；If time conditions cannot meet, sample should be put
Put stored refrigerated in the refrigerator of 4 DEG C.
The yield measuring gained cloud product essential oil of tobaccos is 2.82%.
Embodiment 6
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh simultaneously, and embodiment step is as follows:
(1) cloud product Nicotiana tabacum L. is tentatively pulverized: the cloud dried after weighing a certain amount of cleaning produces Nicotiana tabacum L., at the beginning of carrying out with super micron mill
After step is crushed to 200 mesh, sieve and standby；
(2) cloud produces the pulverizing of Nicotiana tabacum L. microjet: the 200 mesh clouds taking certain step (1) gained produce Nicotiana tabacum L. ultra-micro powder samples, press
It is that 1:60 adds 60% ethanol water immersion according to solid-to-liquid ratio, processes with 100MPa high shear microjet pulverizer after uniform stirring
And circular treatment 20 times；
(3) cloud produce tobacco extract acquisition: by the soak height static pressure pressure of step (2) gained be under 100MPa extract
After 30min, centrifugal 10min under 10000rpm, obtain cloud and produce tobacco extract.
(4) cloud produces the acquisition of essential oil of tobaccos: cloud step (3) obtained produces in tobacco extract and is added into 0.5% β-N-second
Acyl glucosidase, regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation is extracted cloud and produced
Fragrance component in Nicotiana tabacum L., and reclaim fragrance component by liquid nitrogen cold trap absorption；After cooling, carry out breakdown of emulsion with NaCl, then with
Petroleum ether extraction, dries up with nitrogen and obtains cloud product essential oil of tobaccos after petroleum ether removed by instrument；
(5) cloud produces the encapsulation of essential oil of tobaccos: the cloud (4) obtained produces the polyethylene high temperature of essential oil of tobaccos high-temperature sterilization
Retort pouch vacuum seals, and carries out double casing in gnotobasis；
(6) cloud produces the high static pressure sterilization of essential oil of tobaccos: the cloud that is packaged with obtained (5) immediately produces the packaging bag of essential oil of tobaccos
Put into the pressure chamber of high static pressure processing means, and be immersed in pure water transmission medium；Controlling high static pressure sterilization pressure is
500MPa, high static pressure sterilization temperature are 30 DEG C and high static pressure sterilizing time is 40min；Then the Yun Chanyan high static pressure handled well
Grass essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull model calculate microorganism reduce logarithm represent；If time conditions cannot meet, sample should be put
Put stored refrigerated in the refrigerator of 4 DEG C.
The yield measuring gained cloud product essential oil of tobaccos is 2.16%.
Embodiment 7
A kind of cloud produces the high static pressure of essential oil of tobaccos and extracts the method with sterilization fresh simultaneously, and embodiment step is as follows:
(1) cloud product Nicotiana tabacum L. is tentatively pulverized: the cloud dried after weighing a certain amount of cleaning produces Nicotiana tabacum L., at the beginning of carrying out with super micron mill
After step is crushed to 200 mesh, sieve and standby；
(2) cloud produces the pulverizing of Nicotiana tabacum L. microjet: the 200 mesh clouds taking certain step (1) gained produce Nicotiana tabacum L. ultra-micro powder samples, press
It is that 1:50 adds 60% ethanol water immersion according to solid-to-liquid ratio, processes with 100MPa high shear microjet pulverizer after uniform stirring
And circular treatment 20 times；
(3) cloud produce tobacco extract acquisition: by the soak height static pressure pressure of step (2) gained be under 300MPa extract
After 40min, centrifugal 10min under 10000rpm, obtain cloud and produce tobacco extract；
(4) cloud produces the acquisition of essential oil of tobaccos: cloud step (3) obtained produces in tobacco extract and is added into 1.0% β-N-second
Acyl glucosidase, regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation is extracted cloud and produced
Fragrance component in Nicotiana tabacum L., and reclaim fragrance component by liquid nitrogen cold trap absorption；After cooling, carry out breakdown of emulsion with NaCl, then with
Petroleum ether extraction, dries up with nitrogen and obtains cloud product essential oil of tobaccos after petroleum ether removed by instrument；
(5) cloud produces the encapsulation of essential oil of tobaccos: the cloud (4) obtained produces the polyethylene high temperature of essential oil of tobaccos high-temperature sterilization
Retort pouch vacuum seals, and carries out double casing in gnotobasis；
(6) cloud produces the high static pressure sterilization of essential oil of tobaccos: the cloud that is packaged with obtained (5) immediately produces the packaging bag of essential oil of tobaccos
Put into the pressure chamber of high static pressure processing means, and be immersed in pure water transmission medium.Controlling high static pressure sterilization pressure is
400MPa, high static pressure sterilization temperature are 30 DEG C and high static pressure sterilizing time is 30min；Then the Yun Chanyan high static pressure handled well
Grass essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull model calculate microorganism reduce logarithm represent；If time conditions cannot meet, sample should be put
Put stored refrigerated in the refrigerator of 4 DEG C.
The yield measuring gained cloud product essential oil of tobaccos is 3.16%.
As shown in Figure 1-2, preparing cloud product essential oil of tobaccos by this method and be suitable for industrialized production, gained cloud produces Nicotiana tabacum L. essence
Oil yield can improve 1.77-2.64 times than vapor distillation extraction method, can improve 1.55-2.31 than conventional petroleum ether extraction method
Times；Invention gained improves that tobacco aroma organoleptic quality effect is higher than vapor distillation extraction method improves 38.27%, than tradition
Ether soxhlet extraction is the highest improves 42.58%；Gained improves aroma components total concentration than vapor distillation extraction method
The highest improve 30.12%, higher than traditional ether soxhlet extraction improve 24.95%, have and significantly improve tobacco aroma
The effect of organoleptic quality and the obvious effect reducing product preservation term total plate count.
The invention have the benefit that the cloud prepared produces essential oil of tobaccos and has raising cigarette flavor organoleptic quality and increasing
Adding the effect of fragrance constituent concentration, and be suitable for industrialized production, gained cloud produces essential oil of tobaccos yield and extracts than vapor distillation
Method can improve 1.77-2.64 times, can improve 1.55-2.31 times than conventional petroleum ether extraction method；Gained improves tobacco aroma sense organ
Quality effect is higher than vapor distillation extraction method improves 38.27%, higher than traditional ether soxhlet extraction improves
42.58%；Gained improves that aroma components total concentration is higher than vapor distillation extraction method improves 30.12%, than tradition
Ether soxhlet extraction is the highest improves 24.95%, has the effect and significantly significantly improving tobacco aroma organoleptic quality
Reduce the effect of product preservation term total plate count.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, and any
The change expected without creative work or replacement, all should contain within protection scope of the present invention, therefore, and the present invention's
Protection domain should be as the criterion with the protection domain that claims are limited.
Claims (8)
1. the high static pressure of a cloud product essential oil of tobaccos extracts the method with sterilization fresh simultaneously, it is characterised in that include following step
Rapid:
1) cloud product Nicotiana tabacum L. is tentatively pulverized: takes after cloud produces Nicotiana tabacum L. cleaning and dries, is tentatively crushed to 200 mesh with super micron mill
After, sieve and standby；
2) cloud produce Nicotiana tabacum L. microjet pulverize: take step 1) gained 200 mesh clouds produce Nicotiana tabacum L. ultra-micro powder samples, add 60% ethanol
Aqueous solution soaking, processes also circular treatment 20 times with 100MPa high shear microjet pulverizer after uniform stirring；
3) cloud produces the acquisition of tobacco extract: by step 2) after the soak of gained extracts under high static pressure pressure, at 10000r/
Centrifugal 10min under min, obtains cloud and produces tobacco extract；
4) cloud produces the acquisition of essential oil of tobaccos: by step 3) cloud that obtains produces and adds β-N-acetyl glucosaminidase enzyme in tobacco extract,
Regulation pH is 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the reduced steam way of distillation extracts the fragrance one-tenth that cloud produces in Nicotiana tabacum L.
Point, and reclaim fragrance component by liquid nitrogen cold trap absorption, after cooling, carry out breakdown of emulsion with NaCl, then with petroleum ether extraction, use nitrogen
The dry instrument of air-blowing obtains cloud and produces essential oil of tobaccos after removing petroleum ether；
5) cloud produces the encapsulation of essential oil of tobaccos: by 4) cloud that obtains produces the polyethylene high-temperature retort bag of essential oil of tobaccos high-temperature sterilization
Vacuum seals, and carries out double casing in gnotobasis；
6) cloud produce essential oil of tobaccos high static pressure sterilization: immediately to 5) obtain be packaged with cloud produce essential oil of tobaccos packaging bag put into height
In the pressure chamber of static pressure processing means, and it is immersed in pure water transmission medium process, the Yun Chanyan then handled well by high static pressure
Grass essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g or
The logarithm that the microorganism that CFU/mL and Weibull model calculates reduces represents, if time conditions cannot meet, is then put by sample
Put stored refrigerated in the refrigerator of 4 DEG C.
2. the high static pressure that cloud as claimed in claim 1 produces essential oil of tobaccos extracts the method with sterilization fresh simultaneously, and its feature exists
In: in step 2) in, cloud produces Nicotiana tabacum L. ultra-micro powder sample and adds 60% ethanol water leaching according to the solid-to-liquid ratio of 1:40～1:60
Bubble.
3. the high static pressure that cloud as claimed in claim 1 produces essential oil of tobaccos extracts the method with sterilization fresh simultaneously, and its feature exists
In: in step 3) in, soak extracts under the high static pressure pressure of 100～300MPa.
4. the high static pressure that cloud as claimed in claim 3 produces essential oil of tobaccos extracts the method with sterilization fresh simultaneously, and its feature exists
In: in step 3) in, soak extracts 30～60min under high static pressure pressure.
5. the high static pressure that cloud as claimed in claim 1 produces essential oil of tobaccos extracts the method with sterilization fresh simultaneously, and its feature exists
In: in step 4) in, the consumption of β-N-acetyl glucosaminidase enzyme is 0.5%～1.5%.
6. the high static pressure that cloud as claimed in claim 1 produces essential oil of tobaccos extracts the method with sterilization fresh simultaneously, and its feature exists
In: in step 6) in, the high static pressure sterilization pressure of high static pressure processing means is 400-600MPa.
7. the high static pressure that cloud as claimed in claim 6 produces essential oil of tobaccos extracts the method with sterilization fresh simultaneously, and its feature exists
In: in step 6) in, the high static pressure sterilization temperature of high static pressure processing means is 30-50 DEG C.
8. the high static pressure that cloud as claimed in claim 7 produces essential oil of tobaccos extracts the method with sterilization fresh simultaneously, and its feature exists
In: in step 6) in, the high static pressure sterilizing time of high static pressure processing means is 20-40min.
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