CN106108106B - A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh - Google Patents
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh Download PDFInfo
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- CN106108106B CN106108106B CN201610499141.1A CN201610499141A CN106108106B CN 106108106 B CN106108106 B CN 106108106B CN 201610499141 A CN201610499141 A CN 201610499141A CN 106108106 B CN106108106 B CN 106108106B
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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Abstract
The present invention discloses a kind of high static pressure while the method for extraction and sterilization fresh of cloud production essential oil of tobaccos, comprises the following steps：1) cloud production tobacco preliminary crushing；2) cloud production tobacco microjet crushes；3) acquisition of cloud production tobacco extract；4) acquisition of cloud production essential oil of tobaccos；5) encapsulation of cloud production essential oil of tobaccos；6) the high static pressure sterilization of cloud production essential oil of tobaccos；The high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh has process processing simple, non-environmental-pollution, technique is simple, and has efficient, time saving and low cost and other advantages, while cloud production essential oil of tobaccos fragrance component is retained, the shelf-life of product is effectively extended.
Description
Technical field
The invention belongs to active skull cap components extractive technique field, and in particular to a kind of high static pressure of cloud production essential oil of tobaccos is same
When extraction and sterilization fresh method and application.
Background technology
High static pressure (High Hydrostatic Pressure, HHP) refers to the material being sealed in flexible container being placed in
In pressure system, using water or other liquid as transmission medium, using more than 100MPa pressure treatment material, killed with reaching
Bacterium, enzyme deactivation and the purpose for improving material functional characteristic.At present, HHP sterilization technologies are mainly used in the solid-state food containing liquid component
The sterilization of product or food liquid, such as beverage, milk, fruit and vegetable product, dairy produce, meat products sterilization in terms of (《A kind of super-pressure
Homogeneous sterilizes the production method of cow's milk》, the patent No.：201310729089.0；《A kind of sterilization fresh method of wolfberry juice and industry
Change production method》, the patent No.：201510133890.8；《A kind of mulberry jam super-high-pressure sterilizing method》, the patent No.：
201410664252.4；《A kind of Tea flower essence, its preparation method and its application in cigarette》, the patent No.：
201310446881.5) application for, but by it preparing essential oil of tobaccos and sterilization simultaneously is less.In HHP sterilization process, due to
Some material components and structural state are sufficiently complex, therefore to take different treatment conditions according to different material objects.One
As influence HHP sterilization principal element have：Pressure size, pressing time, pressed temperature etc..This experiment attempt using cloud produce tobacco as
Sample, research HHP technologies prepare at the same time extraction cloud production essential oil of tobaccos and sterilization in terms of application, inquire into the antibacterial technology of non-heating power
Application in essential oil of tobaccos produces and stores.
The extracting method of domestic cloud production essential oil of tobaccos mainly has traditional steam distillation, organic solvent extractionprocess at present
Deng, conventional method in the prevalence of it is respective the shortcomings that, as the rate of recovery is low, impurity is more, dissolvent residual and high temperature cause thermal sensitivity into
Destruction divided etc..
The content of the invention
The purpose of the present invention is to overcome the weak point of existing extraction and high-temperature sterilization technique, there is provided a kind of cloud production tobacco essence
The high static pressure of oil is extracted simultaneously and the method for sterilization fresh；Obtain cloud production essential oil of tobaccos have improve cigarette flavor organoleptic quality and
The advantages of increasing fragrance component concentration, and applied in tobacco product；The yield of the method target component is high, and extraction efficiency is high；It is former
Material is by high static pressure extraction and cold sterilization, and process processing is simple, and non-environmental-pollution, technique is simple, and with efficient, time saving and
Low cost and other advantages, while cloud production essential oil of tobaccos fragrance component is retained, the shelf-life of product is effectively extended, this method is
Tobacco business provides a kind of cloud production essential oil of tobaccos that is green, having a extensive future and extracts and improve the technology of preservation term.
In order to solve the problems, such as that the technology of the use of the above-mentioned present invention and method are as follows：
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, comprises the following steps：
1) cloud production tobacco preliminary crushing：Dried after taking cloud production tobacco cleaning, preliminary crushing is carried out to 200 with micronizer
After mesh, sieve and standby；
2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by step 1) to produce tobacco ultra-micro powder sample, add 60%
Ethanol water is soaked, and simultaneously circular treatment 20 times are handled with 100MPa high shear microjets pulverizer after uniform stirring；
3) acquisition of cloud production tobacco extract：After soak obtained by step 2) is extracted under high static pressure pressure,
10min is centrifuged under 10000r/min, obtains cloud production tobacco extract；
4) acquisition of cloud production essential oil of tobaccos：β-N- acetyl glucosamines are added in the cloud production tobacco extract that step 3) is obtained
Glycosides enzyme, regulation pH are 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, in reduced steam way of distillation extraction cloud production tobacco
Fragrance component, and by liquid nitrogen cold trap adsorption recovery fragrance component, after cooling, be demulsified with NaCl, then extracted with petroleum ether
Take, cloud production essential oil of tobaccos is obtained after removing petroleum ether with nitrogen drying instrument；
5) encapsulation of cloud production essential oil of tobaccos：4) cloud obtained production essential oil of tobaccos is used and steamed with the polyethylene high temperature of high-temperature sterilization
A bag vacuum sealing is boiled, double casing is carried out in gnotobasis；
6) the high static pressure sterilization of cloud production essential oil of tobaccos：The packaging bag for being packaged with cloud production essential oil of tobaccos 5) obtained is put immediately
In the pressure chamber for entering high static pressure processing unit, and it is immersed in pure water transmission medium and handles, the cloud for then handling high static pressure well
Produce essential oil of tobaccos sample and carry out microorganism detection according to the detection method of national standard after 24h, testing result is simultaneously with CFU/g
Or the logarithm that the microorganism of CFU/mL and Weibull models calculating reduces represents, if time conditions can not meet, by sample
It is placed on stored refrigerated in 4 DEG C of refrigerator.
Further, in step 2), cloud produces tobacco ultra-micro powder sample according to 1:40~1:60 solid-to-liquid ratio adds
60% ethanol water soaks.
Further, in step 3), soak extracts under 100~300MPa high static pressure pressure.
Further, in step 3), soak extracts 30~60min under high static pressure pressure.
Further, in step 4), the dosage of β-N- acetyl glucosaminidase enzymes is 0.5%~1.5%.
Further, in step 6), the high static pressure sterilization pressure of high static pressure processing unit is 400-600MPa.
Further, in step 6), the high static pressure sterilization temperature of high static pressure processing unit is 30-50 DEG C.
Further, in step 6), the high static pressure sterilizing time of high static pressure processing unit is 20-40min.
Beneficial effects of the present invention are：The cloud production essential oil of tobaccos being prepared, which has, improves cigarette flavor organoleptic quality and increasing
The effect of perfuming gas constituent concentration, and it is suitable for industrialized production, gained cloud produces essential oil of tobaccos yield and extracted than steam distillation
Method can improve 1.77-2.64 times, and 1.55-2.31 times can be improved than conventional petroleum ether extraction method；Gained improves tobacco aroma sense organ
Quality effect can improve 38.27% than steam distillation extraction method highest, can be improved than traditional ether soxhlet extraction highest
42.58%；Gained improves aroma components total concentration can improve 30.12% than steam distillation extraction method highest, than tradition
Ether soxhlet extraction highest can improve 24.95%, with the effect for significantly improving tobacco aroma organoleptic quality and significantly
Reduce the effect of product preservation term total plate count.
Brief description of the drawings
Fig. 1 is the relative value comparison diagram of each embodiment and existing extraction method in the present invention；
Fig. 2 is each embodiment in the present invention and the thermally-sterilized preservation term total plate count comparison diagram of existing routine.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but they are not to skill of the present invention
The restriction of art scheme, based on present invention teach that any conversion made, all falls within protection scope of the present invention.
Embodiment 1
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, step are as follows：
1) cloud production tobacco preliminary crushing：The cloud production tobacco dried after a certain amount of cleaning is weighed, is carried out just with micronizer
After step is crushed to 200 mesh, sieve and standby；
2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by certain step (1) to produce tobacco ultra-micro powder sample, press
It is 1 according to solid-to-liquid ratio:40 add the immersion of 60% ethanol waters, after uniform stirring with 100MPa high shear microjet pulverizers at
Manage simultaneously circular treatment 20 times；
3) acquisition of cloud production tobacco extract：It is to be extracted under 100MPa by the soak high static pressure pressure obtained by step (2)
After 30min, 10min is centrifuged under 10000rpm, obtains cloud production tobacco extract；
4) acquisition of cloud production essential oil of tobaccos：The cloud that step (3) obtains is produced in tobacco extract and is added into 0.5% β-N- acetyl
Glucuroide, regulation pH are 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, reduced steam way of distillation extraction Yun Chanyan
Fragrance component in grass, and pass through liquid nitrogen cold trap adsorption recovery fragrance component；After cooling, it is demulsified with NaCl, then with stone
Oily ether extraction, cloud production essential oil of tobaccos is obtained after removing petroleum ether with nitrogen drying instrument；
5) encapsulation of cloud production essential oil of tobaccos：Polyethylene high temperature of the cloud production essential oil of tobaccos that (4) are obtained with high-temperature sterilization
Retort pouch vacuum sealing, double casing is carried out in gnotobasis；
6) the high static pressure sterilization of cloud production essential oil of tobaccos：(5) are obtained immediately is packaged with the packaging bag of cloud production essential oil of tobaccos
It is put into the pressure chamber of high static pressure processing unit, and is immersed in pure water transmission medium；The high static pressure sterilization pressure is controlled to be
400MPa, high static pressure sterilization temperature are 30 DEG C and high static pressure sterilizing time is 20min；Then cloud high static pressure handled well produces
Essential oil of tobaccos sample carries out microorganism detection according to the detection method of national standard after 24h, and testing result is simultaneously with CFU/g
The logarithm that the microorganism that (or CFU/mL) and Weibull models calculate reduces represents；, should be by sample if time conditions can not meet
Product are placed on stored refrigerated in 4 DEG C of refrigerator.
The yield of measure gained cloud production essential oil of tobaccos is 3.12%.
Embodiment 2
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, embodiment step are as follows：
1) cloud production tobacco preliminary crushing：The cloud production tobacco dried after a certain amount of cleaning is weighed, is carried out just with micronizer
After step is crushed to 200 mesh, sieve and standby；
2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by certain step (1) to produce tobacco ultra-micro powder sample, press
It is 1 according to solid-to-liquid ratio:50 add the immersion of 60% ethanol waters, after uniform stirring with 100MPa high shear microjet pulverizers at
Manage simultaneously circular treatment 20 times；
3) acquisition of cloud production tobacco extract：It is to be extracted under 200MPa by the soak high static pressure pressure obtained by step (2)
After 50min, 10min is centrifuged under 10000rpm, obtains cloud production tobacco extract；
4) acquisition of cloud production essential oil of tobaccos：The cloud that step (3) obtains is produced in tobacco extract and is added into 1.0% β-N- acetyl
Glucuroide, regulation pH are 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, reduced steam way of distillation extraction Yun Chanyan
Fragrance component in grass, and pass through liquid nitrogen cold trap adsorption recovery fragrance component；After cooling, it is demulsified with NaCl, then with stone
Oily ether extraction, cloud production essential oil of tobaccos is obtained after removing petroleum ether with nitrogen drying instrument；
5) encapsulation of cloud production essential oil of tobaccos：Polyethylene high temperature of the cloud production essential oil of tobaccos that (4) are obtained with high-temperature sterilization
Retort pouch vacuum sealing, double casing is carried out in gnotobasis；
6) the high static pressure sterilization of cloud production essential oil of tobaccos：(5) are obtained immediately is packaged with the packaging bag of cloud production essential oil of tobaccos
It is put into the pressure chamber of high static pressure processing unit, and is immersed in pure water transmission medium；The high static pressure sterilization pressure is controlled to be
500MPa, high static pressure sterilization temperature are 40 DEG C and high static pressure sterilizing time is 30min；Then cloud high static pressure handled well produces
Essential oil of tobaccos sample carries out microorganism detection according to the detection method of national standard after 24h, and testing result is simultaneously with CFU/g
The logarithm that the microorganism that (or CFU/mL) and Weibull models calculate reduces represents；, should be by sample if time conditions can not meet
Product are placed on stored refrigerated in 4 DEG C of refrigerator.
The yield of measure gained cloud production essential oil of tobaccos is 3.17%.
Embodiment 3
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, embodiment step are as follows：
1) cloud production tobacco preliminary crushing：The cloud production tobacco dried after a certain amount of cleaning is weighed, is carried out just with micronizer
After step is crushed to 200 mesh, sieve and standby；
2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by certain step (1) to produce tobacco ultra-micro powder sample, press
It is 1 according to solid-to-liquid ratio:60 add the immersion of 60% ethanol waters, after uniform stirring with 100MPa high shear microjet pulverizers at
Manage simultaneously circular treatment 20 times；
3) acquisition of cloud production tobacco extract：It is to be extracted under 300MPa by the soak high static pressure pressure obtained by step (2)
After 30min, 10min is centrifuged under 10000rpm, obtains cloud production tobacco extract；
4) acquisition of cloud production essential oil of tobaccos：The cloud that step (3) obtains is produced in tobacco extract and is added into 0.5% β-N- acetyl
Glucuroide, regulation pH are 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, reduced steam way of distillation extraction Yun Chanyan
Fragrance component in grass, and pass through liquid nitrogen cold trap adsorption recovery fragrance component；After cooling, it is demulsified with NaCl, then with stone
Oily ether extraction, cloud production essential oil of tobaccos is obtained after removing petroleum ether with nitrogen drying instrument；
5) encapsulation of cloud production essential oil of tobaccos：Polyethylene high temperature of the cloud production essential oil of tobaccos that (4) are obtained with high-temperature sterilization
Retort pouch vacuum sealing, double casing is carried out in gnotobasis；
6) the high static pressure sterilization of cloud production essential oil of tobaccos：(5) are obtained immediately is packaged with the packaging bag of cloud production essential oil of tobaccos
It is put into the pressure chamber of high static pressure processing unit, and is immersed in pure water transmission medium；The high static pressure sterilization pressure is controlled to be
600MPa, high static pressure sterilization temperature are 50 DEG C and high static pressure sterilizing time is 20min；Then cloud high static pressure handled well produces
Essential oil of tobaccos sample carries out microorganism detection according to the detection method of national standard after 24h, and testing result is simultaneously with CFU/g
The logarithm that the microorganism that (or CFU/mL) and Weibull models calculate reduces represents；, should be by sample if time conditions can not meet
Product are placed on stored refrigerated in 4 DEG C of refrigerator.
The yield of measure gained cloud production essential oil of tobaccos is 2.67%.
Embodiment 4
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, embodiment step are as follows：
(1) cloud production tobacco preliminary crushing：The cloud production tobacco dried after a certain amount of cleaning is weighed, is carried out just with micronizer
After step is crushed to 200 mesh, sieve and standby；
(2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by certain step (1) to produce tobacco ultra-micro powder sample, press
It is 1 according to solid-to-liquid ratio:40 add the immersion of 60% ethanol waters, after uniform stirring with 100MPa high shear microjet pulverizers at
Manage simultaneously circular treatment 20 times；
(3) acquisition of cloud production tobacco extract：It is to be extracted under 200MPa by the soak high static pressure pressure obtained by step (2)
After 60min, 10min is centrifuged under 10000rpm, obtains cloud production tobacco extract；
(4) acquisition of cloud production essential oil of tobaccos：The cloud that step (3) obtains is produced in tobacco extract and is added into 1.0% β-N- second
Acyl glucuroide, regulation pH are 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the extraction cloud production of the reduced steam way of distillation
Fragrance component in tobacco, and pass through liquid nitrogen cold trap adsorption recovery fragrance component；After cooling, be demulsified with NaCl, then with
Petroleum ether extraction, cloud production essential oil of tobaccos is obtained after removing petroleum ether with nitrogen drying instrument；
(5) encapsulation of cloud production essential oil of tobaccos：Polyethylene high temperature of the cloud production essential oil of tobaccos that (4) are obtained with high-temperature sterilization
Retort pouch vacuum sealing, double casing is carried out in gnotobasis；
(6) the high static pressure sterilization of cloud production essential oil of tobaccos：(5) are obtained immediately is packaged with the packaging bag of cloud production essential oil of tobaccos
It is put into the pressure chamber of high static pressure processing unit, and is immersed in pure water transmission medium；The high static pressure sterilization pressure is controlled to be
600MPa, high static pressure sterilization temperature are 30 DEG C and high static pressure sterilizing time is 30min；Then Yun Chanyan high static pressure handled well
Careless essential oil smaple after 24h according to national standard detection method carry out microorganism detection, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull models calculate microorganism reduce logarithm represent；If time conditions can not meet, sample should be put
Put stored refrigerated in 4 DEG C of refrigerator.
The yield of measure gained cloud production essential oil of tobaccos is 2.45%.
Embodiment 5
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, embodiment step are as follows：
(1) cloud production tobacco preliminary crushing：The cloud production tobacco dried after a certain amount of cleaning is weighed, is carried out just with micronizer
After step is crushed to 200 mesh, sieve and standby；
(2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by certain step (1) to produce tobacco ultra-micro powder sample, press
It is 1 according to solid-to-liquid ratio:50 add the immersion of 60% ethanol waters, after uniform stirring with 100MPa high shear microjet pulverizers at
Manage simultaneously circular treatment 20 times；
(3) acquisition of cloud production tobacco extract：It is to be extracted under 300MPa by the soak high static pressure pressure obtained by step (2)
After 40min, 10min is centrifuged under 10000rpm, obtains cloud production tobacco extract；
(4) acquisition of cloud production essential oil of tobaccos：The cloud that step (3) obtains is produced in tobacco extract and is added into 1.5% β-N- second
Acyl glucuroide, regulation pH are 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the extraction cloud production of the reduced steam way of distillation
Fragrance component in tobacco, and pass through liquid nitrogen cold trap adsorption recovery fragrance component；After cooling, be demulsified with NaCl, then with
Petroleum ether extraction, cloud production essential oil of tobaccos is obtained after removing petroleum ether with nitrogen drying instrument；
(5) encapsulation of cloud production essential oil of tobaccos：Polyethylene high temperature of the cloud production essential oil of tobaccos that (4) are obtained with high-temperature sterilization
Retort pouch vacuum sealing, double casing is carried out in gnotobasis；
(6) the high static pressure sterilization of cloud production essential oil of tobaccos：(5) are obtained immediately is packaged with the packaging bag of cloud production essential oil of tobaccos
It is put into the pressure chamber of high static pressure processing unit, and is immersed in pure water transmission medium；The high static pressure sterilization pressure is controlled to be
400MPa, high static pressure sterilization temperature are 40 DEG C and high static pressure sterilizing time is 20min；Then Yun Chanyan high static pressure handled well
Careless essential oil smaple after 24h according to national standard detection method carry out microorganism detection, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull models calculate microorganism reduce logarithm represent；If time conditions can not meet, sample should be put
Put stored refrigerated in 4 DEG C of refrigerator.
The yield of measure gained cloud production essential oil of tobaccos is 2.82%.
Embodiment 6
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, embodiment step are as follows：
(1) cloud production tobacco preliminary crushing：The cloud production tobacco dried after a certain amount of cleaning is weighed, is carried out just with micronizer
After step is crushed to 200 mesh, sieve and standby；
(2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by certain step (1) to produce tobacco ultra-micro powder sample, press
It is 1 according to solid-to-liquid ratio:60 add the immersion of 60% ethanol waters, after uniform stirring with 100MPa high shear microjet pulverizers at
Manage simultaneously circular treatment 20 times；
(3) acquisition of cloud production tobacco extract：It is to be extracted under 100MPa by the soak high static pressure pressure obtained by step (2)
After 30min, 10min is centrifuged under 10000rpm, obtains cloud production tobacco extract.
(4) acquisition of cloud production essential oil of tobaccos：The cloud that step (3) obtains is produced in tobacco extract and is added into 0.5% β-N- second
Acyl glucuroide, regulation pH are 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the extraction cloud production of the reduced steam way of distillation
Fragrance component in tobacco, and pass through liquid nitrogen cold trap adsorption recovery fragrance component；After cooling, be demulsified with NaCl, then with
Petroleum ether extraction, cloud production essential oil of tobaccos is obtained after removing petroleum ether with nitrogen drying instrument；
(5) encapsulation of cloud production essential oil of tobaccos：Polyethylene high temperature of the cloud production essential oil of tobaccos that (4) are obtained with high-temperature sterilization
Retort pouch vacuum sealing, double casing is carried out in gnotobasis；
(6) the high static pressure sterilization of cloud production essential oil of tobaccos：(5) are obtained immediately is packaged with the packaging bag of cloud production essential oil of tobaccos
It is put into the pressure chamber of high static pressure processing unit, and is immersed in pure water transmission medium；The high static pressure sterilization pressure is controlled to be
500MPa, high static pressure sterilization temperature are 30 DEG C and high static pressure sterilizing time is 40min；Then Yun Chanyan high static pressure handled well
Careless essential oil smaple after 24h according to national standard detection method carry out microorganism detection, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull models calculate microorganism reduce logarithm represent；If time conditions can not meet, sample should be put
Put stored refrigerated in 4 DEG C of refrigerator.
The yield of measure gained cloud production essential oil of tobaccos is 2.16%.
Embodiment 7
A kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, embodiment step are as follows：
(1) cloud production tobacco preliminary crushing：The cloud production tobacco dried after a certain amount of cleaning is weighed, is carried out just with micronizer
After step is crushed to 200 mesh, sieve and standby；
(2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by certain step (1) to produce tobacco ultra-micro powder sample, press
It is 1 according to solid-to-liquid ratio:50 add the immersion of 60% ethanol waters, after uniform stirring with 100MPa high shear microjet pulverizers at
Manage simultaneously circular treatment 20 times；
(3) acquisition of cloud production tobacco extract：It is to be extracted under 300MPa by the soak high static pressure pressure obtained by step (2)
After 40min, 10min is centrifuged under 10000rpm, obtains cloud production tobacco extract；
(4) acquisition of cloud production essential oil of tobaccos：The cloud that step (3) obtains is produced in tobacco extract and is added into 1.0% β-N- second
Acyl glucuroide, regulation pH are 6.0, and under 45 DEG C of temperature conditionss after enzymolysis 4h, the extraction cloud production of the reduced steam way of distillation
Fragrance component in tobacco, and pass through liquid nitrogen cold trap adsorption recovery fragrance component；After cooling, be demulsified with NaCl, then with
Petroleum ether extraction, cloud production essential oil of tobaccos is obtained after removing petroleum ether with nitrogen drying instrument；
(5) encapsulation of cloud production essential oil of tobaccos：Polyethylene high temperature of the cloud production essential oil of tobaccos that (4) are obtained with high-temperature sterilization
Retort pouch vacuum sealing, double casing is carried out in gnotobasis；
(6) the high static pressure sterilization of cloud production essential oil of tobaccos：(5) are obtained immediately is packaged with the packaging bag of cloud production essential oil of tobaccos
It is put into the pressure chamber of high static pressure processing unit, and is immersed in pure water transmission medium.The high static pressure sterilization pressure is controlled to be
400MPa, high static pressure sterilization temperature are 30 DEG C and high static pressure sterilizing time is 30min；Then Yun Chanyan high static pressure handled well
Careless essential oil smaple after 24h according to national standard detection method carry out microorganism detection, testing result simultaneously with CFU/g (or
CFU/mL) and Weibull models calculate microorganism reduce logarithm represent；If time conditions can not meet, sample should be put
Put stored refrigerated in 4 DEG C of refrigerator.
The yield of measure gained cloud production essential oil of tobaccos is 3.16%.
As shown in Figure 1-2, cloud production essential oil of tobaccos is prepared by this method and is suitable for industrialized production, gained cloud production tobacco essence
Oily yield can improve 1.77-2.64 times than steam distillation extraction method, and 1.55-2.31 can be improved than conventional petroleum ether extraction method
Times；Invention gained improves tobacco aroma organoleptic quality effect can improve 38.27% than steam distillation extraction method highest, than tradition
Ether soxhlet extraction highest can improve 42.58%；Gained improves aroma components total concentration than steam distillation extraction method
Highest can improve 30.12%, and 24.95% can be improved than traditional ether soxhlet extraction highest, have and significantly improve tobacco aroma
The effect of organoleptic quality and the obvious effect for reducing product preservation term total plate count.
Beneficial effects of the present invention are：The cloud production essential oil of tobaccos being prepared, which has, improves cigarette flavor organoleptic quality and increasing
The effect of perfuming gas constituent concentration, and it is suitable for industrialized production, gained cloud produces essential oil of tobaccos yield and extracted than steam distillation
Method can improve 1.77-2.64 times, and 1.55-2.31 times can be improved than conventional petroleum ether extraction method；Gained improves tobacco aroma sense organ
Quality effect can improve 38.27% than steam distillation extraction method highest, can be improved than traditional ether soxhlet extraction highest
42.58%；Gained improves aroma components total concentration can improve 30.12% than steam distillation extraction method highest, than tradition
Ether soxhlet extraction highest can improve 24.95%, with the effect for significantly improving tobacco aroma organoleptic quality and significantly
Reduce the effect of product preservation term total plate count.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
The change or replacement expected without creative work, it should all be included within the scope of the present invention, it is therefore, of the invention
Protection domain should be determined by the scope of protection defined in the claims.
Claims (1)
1. a kind of high static pressure of cloud production essential oil of tobaccos is extracted simultaneously and the method for sterilization fresh, it is characterised in that including following step
Suddenly：
1) cloud production tobacco preliminary crushing：Dried after taking cloud production tobacco cleaning, preliminary crushing is carried out to 200 mesh with micronizer
Afterwards, sieve and standby；
2) cloud production tobacco microjet crushes：Take 200 mesh clouds obtained by step 1) to produce tobacco ultra-micro powder sample, add 60% ethanol
Aqueous solution soaking, is handled with 100MPa high shear microjets pulverizer after uniform stirring and circular treatment 20 times；
3) acquisition of cloud production tobacco extract：After soak obtained by step 2) is extracted under high static pressure pressure, in 10000r/
10min is centrifuged under min, obtains cloud production tobacco extract；
4) acquisition of cloud production essential oil of tobaccos：β-N- acetyl glucosaminidase enzymes are added in the cloud production tobacco extract that step 3) is obtained,
Adjust pH be 6.0, and under 45 DEG C of temperature conditionss digest 4h after, the reduced steam way of distillation extraction cloud production tobacco in fragrance into
Point, and by liquid nitrogen cold trap adsorption recovery fragrance component, after cooling, be demulsified with NaCl, then with petroleum ether extraction, use nitrogen
The dry instrument of air-blowing obtains cloud production essential oil of tobaccos after removing petroleum ether；
5) encapsulation of cloud production essential oil of tobaccos：4) cloud obtained production essential oil of tobaccos is used to the polyethylene high-temperature retort bag with high-temperature sterilization
Vacuum sealing, double casing is carried out in gnotobasis；
6) the high static pressure sterilization of cloud production essential oil of tobaccos：Height is put into the packaging bag for being packaged with cloud production essential oil of tobaccos 5) obtained immediately
In the pressure chamber of static pressure processing unit, and it is immersed in pure water transmission medium and handles, the Yun Chanyan for then handling high static pressure well
Careless essential oil smaple carries out microorganism detection according to the detection method of national standard after 24h, testing result simultaneously with CFU/g or
The logarithm that the microorganism that CFU/mL and Weibull models calculate reduces is represented, if time conditions can not meet, sample is put
Put stored refrigerated in 4 DEG C of refrigerator；
In step 2), cloud produces tobacco ultra-micro powder sample according to 1:40~1:60 solid-to-liquid ratio adds the leaching of 60% ethanol water
Bubble；
In step 3), soak extracts under 100~300MPa high static pressure pressure；
In step 3), soak extracts 30~60min under high static pressure pressure；
In step 4), the dosage of β-N- acetyl glucosaminidase enzymes is 0.5%~1.5%；
In step 6), the high static pressure sterilization pressure of high static pressure processing unit is 400-600MPa；
In step 6), the high static pressure sterilization temperature of high static pressure processing unit is 30-50 DEG C；
In step 6), the high static pressure sterilizing time of high static pressure processing unit is 20-40min.
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| CN110574961A (en) * | 2018-06-08 | 2019-12-17 | 湖南中烟工业有限责任公司 | Preparation method of lemon essence for cigarettes, and bead blasting cigarettes for cigarettes |
| CN110574960B (en) * | 2018-06-08 | 2023-06-13 | 湖南中烟工业有限责任公司 | Preparation method of tobacco essence with tobacco flavor and tobacco bead explosion and bead explosion tobacco |
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| CN110655983B (en) * | 2018-06-29 | 2022-11-04 | 湖南中烟工业有限责任公司 | Preparation method and application of essence containing solanesol |
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