CN106108105A - A kind of stems of tobacco leaves prepares the method for strong nut note essence - Google Patents
A kind of stems of tobacco leaves prepares the method for strong nut note essence Download PDFInfo
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- CN106108105A CN106108105A CN201610840552.2A CN201610840552A CN106108105A CN 106108105 A CN106108105 A CN 106108105A CN 201610840552 A CN201610840552 A CN 201610840552A CN 106108105 A CN106108105 A CN 106108105A
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- essence
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- enzymolysis
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
Abstract
The present invention provides a kind of method that stems of tobacco leaves prepares strong nut note essence, comprises the following steps: 1) take cabo powder, add water after stirring, add fungal amylase and pectase carries out first stage enzymolysis；2), after enzyme digestion reaction terminates in the first stage, add protease and carry out second stage enzymolysis；3) after second stage enzyme digestion reaction terminates, product is carried out enzyme denaturing, then cools down and filter, take supernatant, it is thus achieved that cabo enzymolysis solution；4) by cabo enzymolysis solution, add glycine and Semen arachidis hypogaeae powder stirs, carry out Maillard reaction, obtain required essence.Invention further provides the application in Nicotiana tabacum L. of the above-mentioned essence.The method that a kind of stems of tobacco leaves that the present invention provides prepares strong nut note essence, prepared by enzymolysis Maillard reaction, the aroma quality of Medicated cigarette, abundant cigarette perfume, the submissive flue gas of enrichment can be obviously improved, give the nut note that Medicated cigarette is strong, promote the suction quality of Medicated cigarette.
Description
Technical field
The invention belongs to cigarette flavoring essence technical field, relate to a kind of stems of tobacco leaves and prepare the side of strong nut note essence
Method.
Background technology
Nicotiana tabacum L. drafts a document eggplant wood, and Solanaceae is annual or limited herbaceos perennial, base portion slightly lignifying, and inflorescence top is raw, circle
Taper, spends more；Capsule ovum shape or square round shape, length approximates persistent calyx, yields positive results summer and autumn, be distributed mainly on South America, south
Sub-, Chinese.As one hobby property consumer products, Nicotiana tabacum L. has substantial amounts of consumer in each place global.But it is near
Nian Lai, along with the quickening of globalization of world economy process, " smoking is with healthy " problem is increasingly paid close attention to by society, is therefore dropped
Burnt harm reduction becomes the most important thing of cigarette industry.Generally, Medicated cigarette grace can be caused in terms of reducing tar and reducing harm to inhale a large amount of damages of position composition
Losing, cigarette perfume (or spice) is not enough, it is difficult to meet consumer demand.In order to solve problem above, many cigar mills are at allotment cigarette composition
Add substantial amounts of essence simultaneously, although Medicated cigarette so can be made up inhales the deficiency of position, but lack natural sense, have miscellaneous QI to produce.
Cabo refers to stems of tobacco leaves, is i.e. the thick and stiff vein of Nicotiana tabacum L., accounts for leaf and weighs 25%～30%.China is tobacco industry
Big country, cabo, as the by-product of tobacco industry maximum, has few hundred thousand tonnes of to go out of use every year, has the most both caused environmental pollution,
It it is again the waste to natural resources.Research shows, the composition kind contained in cabo is basically identical with tobacco leaf ingredient, and cabo has
Application value realistic greatly.At present, the utilization of cabo is limited only to fill the product such as expanded cut stem, stalk sheet by cigarette industry,
But these products can bring to Medicated cigarette, and a lot of unfavorable aspect, such as cigarette perfume (or spice) are not enough, flue gas is weak, have stimulation, have miscellaneous QI etc..Cause
This, it is necessary to the application to cabo carries out further research and discussion.
Summary of the invention
The shortcoming of prior art in view of the above, it is an object of the invention to provide a kind of stems of tobacco leaves and prepares strong heavily fortified point
The method of fruital rhythm essence, is used for solving to lack in prior art to use with Nicotiana tabacum L. cabo as raw material, through enzymolysis-Mei Lade method
The problem that preparation has the flavouring essence for tobacco of strong nut note.
For achieving the above object and other relevant purposes, first aspect present invention provides a kind of stems of tobacco leaves to prepare strong heavily fortified point
The method of fruital rhythm essence, comprises the following steps:
1) take cabo powder, add water after stirring, add fungal amylase and pectase carries out first stage enzymolysis；
Preferably, described cabo is stems of tobacco leaves.Described Nicotiana tabacum L. is cured tobacco leaf.
Preferably, the preparation method of described cabo powder, for pulverizing after stems of tobacco leaves is toasted, obtain required
Cabo powder.
It is highly preferred that the baking condition of described cabo is: baking temperature: 80-100 DEG C；Baking time: 3-5h.Further
Preferably, described baking condition is: baking temperature: 90 DEG C；Baking time: 4h.
It is highly preferred that the cabo after baking is used pulverizer to be ground into powder by described pulverizing.
Preferably, granularity >=40 mesh of described cabo powder.It is highly preferred that the granularity of described cabo powder is 40 mesh.
Preferably, described water is distilled water.
Preferably, the mass ratio that described cabo powder and water add is 1:9-15.It is highly preferred that described cabo powder with
The mass ratio that water adds is 1:13.
Preferably, the mass ratio that described cabo powder and fungal amylase add is 100:0.05-0.15.More preferably
Ground, the mass ratio that described cabo powder adds with fungal amylase is 100:0.1.
Preferably, described fungal amylase is conventional use of fungal amylase.Described fungal amylase is Isosorbide-5-Nitrae-α-D-
Glucan hydrolase, has another name called fungal alpha-amylase, and No. CAS is 9001-19-8.Specifically, the amylomycin used in the present invention
The traditional fungal amylase (Mycolase) that enzyme is produced by DSM (Chinese) company limited (DSM).
Preferably, the mass ratio that described cabo powder and pectase add is 100:0.05-0.15.It is highly preferred that institute
The mass ratio stating cabo powder and pectase addition is 100:0.1.
Preferably, described pectase is conventional use of pectase.No. CAS of described pectase is 9032-75-1.Specifically
Ground, the pectase used in the present invention is the C80 pectase (Rapidase produced by DSM (Chinese) company limited (DSM)
C80Max)。
Preferably, the reaction condition of described first stage enzymolysis is: hydrolysis temperature: 50-60 DEG C；Enzymolysis time: 2-4h.More
Preferably, the reaction condition of described first stage enzymolysis is: hydrolysis temperature: 55 DEG C；Enzymolysis time: 3h.
2) in step 1) described in first stage enzyme digestion reaction terminate after, add protease and carry out second stage enzymolysis；
Preferably, described protease is conventional use of protease.No. CAS of described protease is 9014-01-1.Specifically
Ground, the traditional protease that the protease used in the present invention is produced by DSM (Chinese) company limited (DSM)
(MaxiproNPU)。
Preferably, the mass ratio that described cabo powder and protease add is 100:0.1-0.3.It is highly preferred that it is described
The mass ratio that cabo powder adds with protease is 100:0.2.
Preferably, the reaction condition of described second stage enzymolysis is: hydrolysis temperature: 50-60 DEG C；Enzymolysis time: 2-4h.More
Preferably, the reaction condition of described second stage enzymolysis is: hydrolysis temperature: 55 DEG C；Enzymolysis time: 3h.
3) in step 2) described in second stage enzyme digestion reaction terminate after, product is carried out enzyme denaturing, then cools down and filter, take
Supernatant, it is thus achieved that cabo enzymolysis solution；
Preferably, the reaction condition of described enzyme denaturing is: enzyme-removal temperature: 80-90 DEG C；Enzyme denaturing time: 10-20min.More preferably
Ground, the reaction condition of described enzyme denaturing is: enzyme-removal temperature: 85 DEG C；Enzyme denaturing time: 15min.
Preferably, described filtration uses sucking filtration mode to carry out.
4) by step 3) the middle cabo enzymolysis solution that obtains, addition glycine and Semen arachidis hypogaeae powder stir, and carry out Mei Lade anti-
Should, obtain required essence.
Preferably, the mass ratio that described cabo powder and glycine add is 100:2-4.It is highly preferred that described cabo
The mass ratio that powder adds with glycine is 100:3.
Preferably, the mass ratio that described cabo powder and Semen arachidis hypogaeae powder add is 10:2-8.It is highly preferred that described cabo
The mass ratio that powder adds with Semen arachidis hypogaeae powder is 10:4-6.
Preferably, the preparation method of described Semen arachidis hypogaeae powder, pulverize after toasting for the Semen arachidis hypogaeae after frying, i.e.
Semen arachidis hypogaeae powder needed for.
It is highly preferred that the baking condition of described Semen arachidis hypogaeae is: baking temperature: 110-130 DEG C；Baking time: 1-3h.Enter one
Preferably, described baking condition is step: baking temperature: 120 DEG C；Baking time: 2h.
It is highly preferred that the Semen arachidis hypogaeae after baking is used crusher to be ground into powder by described pulverizing.
Preferably, granularity >=60 mesh of described Semen arachidis hypogaeae powder.It is highly preferred that the granularity of described Semen arachidis hypogaeae powder is 60 mesh.
Preferably, the condition of described Maillard reaction is: reaction temperature: 80-120 DEG C；Response time: 2-4h.More preferably
Ground, the condition of described Maillard reaction is: reaction temperature: 100 DEG C；Response time: 3h.
Preferably, described essence is flavouring essence for tobacco, has strong nut note.
Second aspect present invention provides the strong nut note essence prepared by said method.
Third aspect present invention provides a kind of strong nut note essence purposes in Nicotiana tabacum L..
Preferably, described Nicotiana tabacum L. is cured tobacco leaf.
Fourth aspect present invention provides the using method of a kind of strong nut note essence, for by dilute for above-mentioned essence ethanol
After releasing, weigh essence diluent and cured tobacco leaf in proportion, essence diluent is uniformly sprayed in cured tobacco leaf.
Preferably, described ethanol is edible ethanol, and described ethanol is the aqueous solution of the dehydrated alcohol containing conventional ratio.More excellent
Selection of land, described ethanol is the edible ethanol containing percent by volume 70% dehydrated alcohol.
Preferably, described essence diluent is the ethanol solution containing conventional ratio essence.It is highly preferred that the dilution of described essence
In liquid, the mass percent shared by essence is 10%.
Preferably, described essence diluent and cured tobacco leaf additional proportion are conventional mass ratio.It is highly preferred that it is described
The mass ratio that essence diluent adds with cured tobacco leaf is 5:1000.
Preferably, described cured tobacco leaf is the cured tobacco leaf of Yunnan Yuxi's board Medicated cigarette.
Preferably, described sprinkling uses medical spray gun to carry out.
As it has been described above, the method that a kind of stems of tobacco leaves that the present invention provides prepares strong nut note essence, by cigarette
The front extract of the reactive essence of leaf stem enzyme digestion reaction preparation, carries out Maillard reaction obtain having dense through adding Semen arachidis hypogaeae powder
The flavouring essence for tobacco of Yu Jianguo note.Flavouring essence for tobacco prepared by the present invention, with Nicotiana tabacum L. cabo as raw material, abundant raw material source, technique
Simply, easily operate.Flavouring essence for tobacco prepared by the present invention, compared with traditional perfumery essence, can be obviously improved the aroma quality of Medicated cigarette
Amount, abundant cigarette are fragrant, enrichment thickens flue gas, give the nut note that Medicated cigarette is strong, promote the suction quality of Medicated cigarette, it is possible to effectively change
Enter the shortcoming such as the cigarette perfume (or spice) deficiency of existing general Medicated cigarette, weak, the nutty dimness of flue gas, can be widely used in cigarette flavoring essence field
In.
Accompanying drawing explanation
Fig. 1 is shown as the GC collection of illustrative plates of the cabo enzymolysis solution that cabo only obtains in the present invention through enzyme digestion reaction.
Fig. 2 is shown as the essence (formula being not added with Semen arachidis hypogaeae powder that in the present invention, cabo obtains through enzymolysis-Maillard reaction
12) GC collection of illustrative plates.
Fig. 3 is shown as the essence (formula adding Semen arachidis hypogaeae powder that in the present invention, cabo obtains through enzymolysis-Maillard reaction
16) GC collection of illustrative plates.
Fig. 4 is shown as the GC collection of illustrative plates of stem extracting solution in the present invention.
Detailed description of the invention
The present invention is expanded on further, it should be appreciated that these embodiments are merely to illustrate the present invention below in conjunction with specific embodiment
Rather than limit the scope of the invention.
Below by way of specific instantiation, embodiments of the present invention being described, those skilled in the art can be by this specification
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also be by the most different concrete realities
The mode of executing is carried out or applies, the every details in this specification can also based on different viewpoints and application, without departing from
Various modification or change is carried out under the spirit of the present invention.
It should be clear that conventional equipment in the concrete process equipment indicated or device all use this area in the following example or
Device；All force value and scope are all referring to relative pressure, the routine that the instrument of present invention use or reagent are in this area
Instrument or reagent, the most commercially available acquisition.
In addition, it is to be understood that the one or more method steps mentioned in the present invention do not repel before and after described combination step
Additive method step can also be there is or additive method step can also be inserted between these steps specifically mentioned, unless separately
It is described；Should also be understood that the combination annexation between the one or more equipment/devices mentioned in the present invention is not repelled
Can also exist before and after described unit equipment/device other equipment/devices or these two equipment/devices specifically mentioned it
Between can also insert other equipment/devices, except as otherwise noted.And, except as otherwise noted, the numbering of various method steps is only
Differentiate the convenient tool of various method steps, rather than for limiting the ordering of various method steps or limiting the enforceable model of the present invention
Enclose, being altered or modified of its relativeness, in the case of without essence change technology contents, enforceable when being also considered as the present invention
Category.
Embodiment 1
Stems of tobacco leaves is pulverized after 80-100 DEG C of baking 3-5h, it is thus achieved that required cabo powder.Take a certain amount of cabo
Powder, after addition distilled water stirs, adds fungal amylase and C80 pectase carries out first stage enzyme at 50-60 DEG C
Solve 2-4h.Wherein, the mass ratio that cabo powder and distilled water add is 1:9-15；Cabo powder adds with fungal amylase
Mass ratio is 100:0.05-0.15；The mass ratio that cabo powder adds with C80 pectase is 100:0.05-0.15.
Then, adding protease and carry out second stage enzymolysis 2-4h at 50-60 DEG C, wherein, cabo powder adds with protease
The mass ratio entered is 100:0.1-0.3.Enzymatic hydrolysate is carried out enzyme denaturing 10-20min at 80-90 DEG C, then cools down and filter, take
Supernatant, it is thus achieved that cabo enzymolysis solution.Wherein, total enzyme digestion reaction time of first stage enzymolysis and second stage enzymolysis is 4-8h.
Finally by cabo enzymolysis solution, add glycine and Semen arachidis hypogaeae powder stirs, carry out Mei Lade at 80-120 DEG C anti-
Answer 2-4h, obtain required essence.Wherein, the mass ratio that cabo powder and glycine add is 100:2-4；Cabo powder and flower
The mass ratio that green powder adds is 10:2-8.Semen arachidis hypogaeae powder is that the Semen arachidis hypogaeae after frying carries out toasting 1-at 110-130 DEG C
Pulverize after 3h and obtain.
When above-mentioned essence uses in Nicotiana tabacum L., by above-mentioned essence with the edible ethanol containing percent by volume 70% dehydrated alcohol
After dilution, it is made into the essence diluent that the mass percent shared by essence is 10%, then adds with cured tobacco leaf by essence diluent
The mass ratio entered is that 5:1000 weighs essence diluent and cured tobacco leaf, uses medical spray gun uniformly to be sprayed by essence diluent
It is sprinkled upon in cured tobacco leaf.
Embodiment 2
Carry out during enzymolysis-Maillard reaction prepares essence in stems of tobacco leaves, in the case of without Semen arachidis hypogaeae powder,
The consumption of distilled water in enzyme digestion reaction is inquired into, is obtained the different distilled water consumption impact on product fragrance, from
And judging optimal distilled water consumption, concrete preparation process is as follows.
Stems of tobacco leaves is pulverized after 90 DEG C of baking 4h, it is thus achieved that required cabo powder.Take 10.00g cabo powder, point
Do not add after the distilled water of different amounts stirs, add 0.01g fungal amylase and 0.01g C80 pectase at 55 DEG C
Carry out first stage enzymolysis 2h.Wherein, the quality that distilled water adds is respectively 90.00g, 110.00g, 130.00g, 150.00g.
Then, add 0.02g protease and carry out second stage enzymolysis 2h at 55 DEG C, enzymatic hydrolysate is carried out enzyme denaturing at 85 DEG C
15min, then cool down and filter, take supernatant, it is thus achieved that cabo enzymolysis solution.Wherein, first stage enzymolysis and second stage enzymolysis
Total enzyme digestion reaction time is 4h.
Finally by cabo enzymolysis solution, add 0.30g glycine and stir, carry out Maillard reaction 3h at 100 DEG C, i.e. divide
Not Huo get essence 1#, 2#, 3#, 4#, the formula 1,2,3,4 of essence 1#, 2#, 3#, 4#, be shown in Table 1.
The different distilled water consumption impact on product fragrance of table 1
As shown in Table 1, in formula 1, the consumption of distilled water controls about 9 times at cabo powder, by being sprayed at by product
On the cured tobacco leaf of Yunnan Yuxi's board Medicated cigarette, finding after perfumer aspirates, fragrance is inharmonious with cigarette perfume (or spice), puckery, has miscellaneous QI；Formula
In 2, the consumption of distilled water controls, at about 11 times of cabo powder, by suction, to feel that cigarette perfume slightly increases than formula 1, but cigarette
Gas is weak, lacks cigarette strength；In formula 3, the consumption of distilled water controls, at about 13 times of cabo powder, by suction, to feel that cigarette is fragrant
Relatively sufficient, flue gas is also dense than the first two formula, but burnt fragrant and sweet the most weak；In formula 4, the consumption of distilled water controls at cabo powder
About 15 times, by suction, cigarette is fragrant similar with formula 3, but has Jiao's hardship gas.By above 4 recipe ratios relatively, formula 3
The consumption of middle distilled water controls 13 times at cabo powder, is the optimum amount of distilled water, and its overall full aroma, flue gas is more
Dense, but burnt fragrant and sweet the most weak, and this may be relevant with the allotment in response time and later stage.
Embodiment 3
Carry out during enzymolysis-Maillard reaction prepares essence in stems of tobacco leaves, in the case of without Semen arachidis hypogaeae powder,
Response time in enzyme digestion reaction is inquired into, is obtained the different enzyme digestion reaction time impact on product fragrance, thus
Judging the optimal enzyme digestion reaction time, concrete preparation process is as follows.
Stems of tobacco leaves is pulverized after 90 DEG C of baking 4h, it is thus achieved that required cabo powder.Take 10.00g cabo powder, add
Enter after 130.00g distilled water stirs, add 0.01g fungal amylase and 0.01g C80 pectase enters respectively at 55 DEG C
Row first stage enzymolysis 2h, 3h, 4h.
Then, add 0.02g protease and carry out second stage enzymolysis 2h, 3h, 4h respectively at 55 DEG C, by enzymatic hydrolysate 85
DEG C carry out enzyme denaturing 15min, then cool down and filter, take supernatant, it is thus achieved that cabo enzymolysis solution.Wherein, first stage enzymolysis and second
Total enzyme digestion reaction time of stage enzymolysis is respectively 4h, 6h, 8h.
Finally by cabo enzymolysis solution, add 0.30g glycine and stir, carry out Maillard reaction 3h at 100 DEG C, i.e. divide
Not Huo get essence 5#, 6#, 7#, the formula 5,6,7 of essence 5#, 6#, 7#, be shown in Table 2.
The different enzyme digestion reaction time impact on product fragrance of table 2
As shown in Table 2, in formula 5, total enzyme digestion reaction time controls at about 4h, by product is sprayed at Yunnan Yuxi
On the cured tobacco leaf of board Medicated cigarette, finding after perfumer aspirates, fragrance is the fullest, but lacks charm；In formula 6, total enzymolysis is anti-
Controlling at about 6h between Ying Shi, by suction, full aroma degree increases, and flue gas is the denseest, has obvious burnt fragrant and sweet rhythm；Join
In side 7, total enzyme digestion reaction time controls at about 8h, and by suction, fragrance is poor, stimulates relatively big, and aftertaste is dry and astringent.By above 3
Individual recipe ratio relatively understands, and in formula 6, total enzyme digestion reaction time controls at 6h, is the optimal enzyme digestion reaction time, the product now drawn
In thing, nutty rhythm is the most weak, and this needs to add a little natural nuts raw material during Maillard reaction, increases a little baking
Fragrance, makes fragrance more coordinate full.
Embodiment 4
Carry out during enzymolysis-Maillard reaction prepares essence in stems of tobacco leaves, in the case of without Semen arachidis hypogaeae powder,
Reaction temperature in Maillard reaction is inquired into, is obtained the different Maillard reaction temperature impact on product fragrance,
Thus judging optimal Maillard reaction temperature, concrete preparation process is as follows.
Stems of tobacco leaves is pulverized after 90 DEG C of baking 4h, it is thus achieved that required cabo powder.Take 10.00g cabo powder, add
Enter after 130.00g distilled water stirs, add 0.01g fungal amylase and 0.01g C80 pectase enters respectively at 55 DEG C
Row first stage enzymolysis 3h.
Then, add 0.02g protease and carry out second stage enzymolysis 3h respectively at 55 DEG C, enzymatic hydrolysate is carried out at 85 DEG C
Enzyme denaturing 15min, then cool down and filter, take supernatant, it is thus achieved that cabo enzymolysis solution.Wherein, first stage enzymolysis and second stage enzyme
The total enzyme digestion reaction time solved is respectively 6h.
Finally by cabo enzymolysis solution, add 0.30g glycine and stir, at a temperature of differential responses, carry out Mei Lade anti-
Answer 3h, obtain essence 8#, 9#, 10#, the formula 8,9,10 of essence 8#, 9#, 10# the most respectively, be shown in Table 3.Wherein, Maillard reaction
Temperature is respectively 80 DEG C, 100 DEG C, 120 DEG C.
The different Maillard reaction temperature impact on product fragrance of table 3
As shown in Table 3, in formula 8, Maillard reaction temperature controls at about 80 DEG C, beautiful by product is sprayed at Yunnan
On the cured tobacco leaf of small stream board Medicated cigarette, finding after perfumer aspirates, fragrance is the most inharmonious with cigarette perfume (or spice), has xylon gas；In formula 9
Maillard reaction temperature controls at about 100 DEG C, and by suction, fragrance is coordinated with cigarette perfume (or spice), and flue gas is the denseest, has significantly
Burnt fragrant and sweet rhythm；In formula 10, Maillard reaction temperature controls at about 120 DEG C, and by suction, fragrance is poor, has burnt bitter gas.Logical
Crossing above 3 recipe ratios relatively to understand, in formula 9, Maillard reaction temperature controls at 100 DEG C, is optimal Maillard reaction temperature.
Embodiment 5
Carry out during enzymolysis-Maillard reaction prepares essence in stems of tobacco leaves, in the case of without Semen arachidis hypogaeae powder,
Response time in Maillard reaction is inquired into, is obtained the different Maillard reaction time impact on product fragrance,
Thus judging the optimal Maillard reaction time, concrete preparation process is as follows.
Stems of tobacco leaves is pulverized after 90 DEG C of baking 4h, it is thus achieved that required cabo powder.Take 10.00g cabo powder, add
Enter after 130.00g distilled water stirs, add 0.01g fungal amylase and 0.01g C80 pectase enters respectively at 55 DEG C
Row first stage enzymolysis 3h.
Then, add 0.02g protease and carry out second stage enzymolysis 3h respectively at 55 DEG C, enzymatic hydrolysate is carried out at 85 DEG C
Enzyme denaturing 15min, then cool down and filter, take supernatant, it is thus achieved that cabo enzymolysis solution.Wherein, first stage enzymolysis and second stage enzyme
The total enzyme digestion reaction time solved is respectively 6h.
Finally by cabo enzymolysis solution, add 0.30g glycine and stir, at 100 DEG C, carry out U.S. of different time sections
Maillard reaction, obtains essence 11#, 12#, 13#, the formula 11,12,13 of essence 11#, 12#, 13# the most respectively, is shown in Table 4.Wherein,
The Maillard reaction time is respectively 2h, 3h, 4h.
The different Maillard reaction time impact on product fragrance of table 4
As shown in Table 4, in formula 11, the Maillard reaction time controls at about 2h, by product is sprayed at Yunnan Yuxi
On the cured tobacco leaf of board Medicated cigarette, finding after perfumer aspirates, fragrance is the fullest, and fragrant and sweet rhythm is more weak, inharmonious with cigarette perfume (or spice)；Formula
In 12, the Maillard reaction time controls at about 3h, and by suction, fragrant and sweet rhythm the most somewhat weakness, but the obvious enrichment of flue gas increases
Thickness, fuller than flue gas before；In formula 13, the Maillard reaction time controls at about 4h, and by suction, cigarette perfume (or spice) is not enough, cigarette
Gas is weak, has miscellaneous QI.By above 3 recipe ratios relatively, in formula 12, the Maillard reaction time controls at 3h, Shi Jiamei
The Maillard reaction time.
Embodiment 6
Carry out during enzymolysis-Maillard reaction prepares essence in stems of tobacco leaves, in order to increase the strong nut of essence
Note, can add Semen arachidis hypogaeae powder, therefore, it is necessary to inquired into the consumption of Semen arachidis hypogaeae powder, obtains Semen arachidis hypogaeae powder not
With the consumption impact on product fragrance, thus judging the consumption of optimal Semen arachidis hypogaeae powder, concrete preparation process is as follows.
Stems of tobacco leaves is pulverized after 90 DEG C of baking 4h, it is thus achieved that required cabo powder.Take 10.00g cabo powder, add
Enter after 130.00g distilled water stirs, add 0.01g fungal amylase and 0.01g C80 pectase enters respectively at 55 DEG C
Row first stage enzymolysis 3h.
Then, add 0.02g protease and carry out second stage enzymolysis 3h respectively at 55 DEG C, enzymatic hydrolysate is carried out at 85 DEG C
Enzyme denaturing 15min, then cool down and filter, take supernatant, it is thus achieved that cabo enzymolysis solution.Wherein, first stage enzymolysis and second stage enzyme
The total enzyme digestion reaction time solved is respectively 6h.
Finally by cabo enzymolysis solution, the Semen arachidis hypogaeae powder adding 0.30g glycine and different content stirs, at 100 DEG C
Under carry out the Maillard reaction of different time sections, obtain essence 14#, 15#, 16#, 17# the most respectively, essence 14#, 15#, 16#,
The formula 14,15,16,17 of 17#, is shown in Table 5.Wherein, the consumption of Semen arachidis hypogaeae powder is respectively 2g, 4g, 6g, 8g.
The impact on product fragrance of the addition of the different Semen arachidis hypogaeae powder of table 5
As shown in Table 5, in formula 14, stems of tobacco leaves powder controls at about 10:2 with the mass ratio of Semen arachidis hypogaeae powder consumption,
By product being sprayed on the cured tobacco leaf of Yunnan Yuxi's board Medicated cigarette, through perfumer aspirate after find, fragrance not circle and, cigarette
Gas is weak, and nut note is not enough；In formula 15, stems of tobacco leaves powder controls at 10:4 left with the mass ratio of Semen arachidis hypogaeae powder consumption
The right side, by smelling, flue gas is the denseest, has the most burnt fragrant and sweet, and nut note is also than formula 14 foot；Stems of tobacco leaves in formula 16
Powder controls at about 10:6 with the mass ratio of Semen arachidis hypogaeae powder consumption, and by suction, flue gas is the denseest, fine and smooth, has substantially
Baking is fragrant and nutty；In formula 17, stems of tobacco leaves powder controls at about 10:8 with the mass ratio of Semen arachidis hypogaeae powder consumption,
By suction, flue gas is rougher, though there being nutty, but has miscellaneous QI, stimulation.By above 4 recipe ratios relatively, stems of tobacco leaves
The mass ratio of powder and Semen arachidis hypogaeae powder consumption controls between 10:4～10:6, it is possible to obtain optimal Semen arachidis hypogaeae powder consumption.
Embodiment 7
In order to examine or check the preparation method impact on essence quality, respectively by preparing stem extracting solution, only through enzyme digestion reaction
The cabo enzymolysis solution that obtains, the essence being not added with Semen arachidis hypogaeae powder obtained through enzymolysis-Maillard reaction, anti-through enzymolysis-Mei Lade
The essence adding Semen arachidis hypogaeae powder that should obtain, compares.
First, stem extracting solution is prepared: will stems of tobacco leaves pulverize after 90 DEG C of baking 4h, it is thus achieved that required cabo powder
End.Taking 10.00g cabo powder, after addition 130.00g distilled water stirs, extract 4h at 70 DEG C, sucking filtration obtains cabo and extracts
Liquid.
Secondly, the cabo enzymolysis solution that preparation only obtains through enzyme digestion reaction: will stems of tobacco leaves carry out after 90 DEG C of baking 4h
Pulverize, it is thus achieved that required cabo powder.Take 10.00g cabo powder, after addition 130.00g distilled water stirs, add
0.01g fungal amylase and 0.01g C80 pectase carry out first stage enzymolysis 3h respectively at 55 DEG C.Add 0.02g albumen
Enzyme carries out second stage enzymolysis 3h respectively at 55 DEG C, at 85 DEG C, enzymatic hydrolysate is carried out enzyme denaturing 15min, then cools down and filter, take
Supernatant, it is thus achieved that cabo enzymolysis solution.Wherein, total enzyme digestion reaction time of first stage enzymolysis and second stage enzymolysis is respectively
6h。
Then, the essence being not added with Semen arachidis hypogaeae powder that preparation obtains through enzymolysis-Maillard reaction: its preparation method is with implementing
Formula 12 in example 5.
Finally, the essence adding Semen arachidis hypogaeae powder that preparation obtains through enzymolysis-Maillard reaction: the same embodiment of its preparation method
Formula 16 in 6.
Above-mentioned by acquisition prepares reactant liquor, carries out GC-MS detection respectively, and the testing result of stem extracting solution is shown in Fig. 4, table
6, the testing result of the cabo enzymolysis solution only obtained through enzyme digestion reaction is shown in Fig. 1, table 6, through not adding that enzymolysis-Maillard reaction obtains
Testing result with the essence of Semen arachidis hypogaeae powder is shown in Fig. 2, table 6, through the essence adding Semen arachidis hypogaeae powder that enzymolysis-Maillard reaction obtains
Testing result see Fig. 3, table 6.
Wherein, GC-MS testing conditions is:
GC condition: chromatographic column: INNOWAX, 60m × 0.25mm × 0.25 μm；Carrier gas: He；Flow rate of carrier gas: 1mL/min；Enter
Original mold formula: do not shunt；Injector temperature: 250 DEG C；Heating schedule: 40 DEG C keep 6min, rise to 100 DEG C with 3 DEG C/min, then with 5
DEG C/min rise to 230 DEG C keep 10min；
MS condition: ion source: EI source；Ion source temperature: 230 DEG C；Electron energy: 70eV；Mass scan range: 30～
350amu；Scan pattern: full scan.
As shown in Table 6, (1) and cabo enzymolysis solution, cabo enzymolysis-Maillard reaction essence, cabo enzymolysis-Maillard reaction
And add peanut essence and compare, in stem extracting solution, aroma-producing substance kind significantly reduces, and content is relatively low, the most slightly contains a bit
Baking fragrance.(2) correction data from the content of furan derivatives, phenol derivatives and 2-acetyl pyrrole can draw, former
Material stem after enzymolysis and Maillard reaction, cigarette perfume material kind showed increased, and baking is fragrant, cigarette perfume (or spice) component content raises, blue or green
Gas, abnormal flavour, miscellaneous QI component content reduce, and illustrate after enzymolysis and Maillard reaction, the flavor matter showed increased of raw material stem,
In flavouring essence for tobacco, cigarette perfume (or spice) is not enough, have the shortcoming such as abnormal flavour, miscellaneous QI to solve it, but nutty composition still has deficiency.(3) will process
The Semen arachidis hypogaeae powder crossed is added in the Maillard reaction of stem, and the content of nutty composition substantially increases, and baking is fragrant and causes perfume one-tenth
Divide content the most not reduce, the most slightly raise, illustrate that the enzymolysis-Maillard reaction liquid of the stems of tobacco leaves of the present invention has dense
Strongly fragrant nutty, baking note, can effectively promote that cigarette flavor matter, abundant cigarette be fragrant, enrichment thickens flue gas, has and improves Medicated cigarette and take out
Inhale the effect of quality.
Fragrance component title and content thereof in table 6 differential responses liquid
So, the present invention effectively overcomes various shortcoming of the prior art and has high industrial utilization.
The principle of above-described embodiment only illustrative present invention and effect thereof, not for limiting the present invention.Any ripe
Above-described embodiment all can be modified under the spirit and the scope of the present invention or change by the personage knowing this technology.Cause
This, have usually intellectual such as complete with institute under technological thought without departing from disclosed spirit in art
All equivalences become are modified or change, and must be contained by the claim of the present invention.
Claims (10)
1. the method that stems of tobacco leaves prepares strong nut note essence, comprises the following steps:
1) take cabo powder, add water after stirring, add fungal amylase and pectase carries out first stage enzymolysis；
2) in step 1) described in first stage enzyme digestion reaction terminate after, add protease and carry out second stage enzymolysis；
3) in step 2) described in second stage enzyme digestion reaction terminate after, product is carried out enzyme denaturing, then cools down and filter, take supernatant
Liquid, it is thus achieved that cabo enzymolysis solution；
4) by step 3) the middle cabo enzymolysis solution that obtains, addition glycine and Semen arachidis hypogaeae powder stir, and carry out Maillard reaction, i.e.
Essence needed for.
A kind of stems of tobacco leaves the most according to claim 1 prepares the method for strong nut note essence, it is characterised in that step
Rapid 1) in, the preparation method of described cabo powder, for pulverizing after stems of tobacco leaves being toasted, obtain required cabo powder
End.
A kind of stems of tobacco leaves the most according to claim 2 prepares the method for strong nut note essence, it is characterised in that institute
The baking condition stating cabo is: baking temperature: 80-100 DEG C；Baking time: 3-5h.
A kind of stems of tobacco leaves the most according to claim 1 prepares the method for strong nut note essence, it is characterised in that step
Rapid 1) in, including any one of following condition or multinomial:
A1) mass ratio that described cabo powder and water add is 1:9-15；
A2) mass ratio that described cabo powder and fungal amylase add is 100:0.05-0.15；
A3) mass ratio that described cabo powder and pectase add is 100:0.05-0.15；
A4) reaction condition of described first stage enzymolysis is: hydrolysis temperature: 50-60 DEG C；Enzymolysis time: 2-4h.
A kind of stems of tobacco leaves the most according to claim 1 prepares the method for strong nut note essence, it is characterised in that step
Rapid 2) in, including any one of following condition or multinomial:
B1) mass ratio that described cabo powder and protease add is 100:0.1-0.3；
B2) reaction condition of described second stage enzymolysis is: hydrolysis temperature: 50-60 DEG C；Enzymolysis time: 2-4h.
A kind of stems of tobacco leaves the most according to claim 1 prepares the method for strong nut note essence, it is characterised in that step
Rapid 3), in, the reaction condition of described enzyme denaturing is: enzyme-removal temperature: 80-90 DEG C；Enzyme denaturing time: 10-20min.
A kind of stems of tobacco leaves the most according to claim 1 prepares the method for strong nut note essence, it is characterised in that step
Rapid 4) in, including any one of following condition or multinomial:
C1) mass ratio that described cabo powder and glycine add is 100:2-4；
C2) mass ratio that described cabo powder and Semen arachidis hypogaeae powder add is 10:2-8；
C3) condition of described Maillard reaction is: reaction temperature: 80-120 DEG C；Response time: 2-4h.
8. a strong nut note essence, prepares according to the arbitrary described preparation method of claim 1-7.
A kind of strong nut note essence the most according to claim 8 purposes in Nicotiana tabacum L..
The using method of a kind of strong nut note essence the most according to claim 8, for by dilute for above-mentioned essence ethanol
After releasing, weigh essence diluent and cured tobacco leaf in proportion, essence diluent is uniformly sprayed in cured tobacco leaf.
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| CN112220099A (en) * | 2020-09-23 | 2021-01-15 | 云南中烟工业有限责任公司 | Method for preparing spice with nut fragrance and hay fragrance, spice and application thereof |
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