CN106106449A - Terbinafine formulation and the application in improving Nicotiana tabacum L. Squalene content thereof - Google Patents
Terbinafine formulation and the application in improving Nicotiana tabacum L. Squalene content thereof Download PDFInfo
- Publication number
- CN106106449A CN106106449A CN201610454678.6A CN201610454678A CN106106449A CN 106106449 A CN106106449 A CN 106106449A CN 201610454678 A CN201610454678 A CN 201610454678A CN 106106449 A CN106106449 A CN 106106449A
- Authority
- CN
- China
- Prior art keywords
- nicotiana tabacum
- terbinafine
- formulation
- strain
- squalene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
Abstract
The invention discloses a kind of Terbinafine formulation and the application in improving Nicotiana tabacum L. Squalene content thereof.The Terbinafine formulation of the present invention is made up of the component of following percetage by weight: terbinafine 10～20%；Three (laureth 4) phosphate ester 5～8%；Surplus is ethanol.In the growth course of Nicotiana tabacum L., add the Terbinafine formulation of the present invention, it is possible to increase the content of Squalene in Nicotiana tabacum L., improve the economic worth of Nicotiana tabacum L..
Description
Technical field
The present invention relates to tobacco planting field, more particularly, to a kind of Terbinafine formulation and at raising Nicotiana tabacum L. angle
Application in squalene content.
Background technology
Squalene is a kind of lipid non-saponifiable matter, for a kind of many unsaturated olefins (squalene), is initially from shark
Liver oil in find, within 1914, be named as Squalene, its chemical name is 2,6,10,15,19,23-hexamethyl-2,6,
10,14,18,22-tetracosa carbon six alkene, belong to open chain triterpene, also known as Squalene, participate in human body cholesterol biosynthesis and can promote new
Old metabolism, has raising internal superoxide dismutase (SOD) activity, enhancing human body immunity ability, defying age, resisting fatigue, resists
The different physiological roles such as tumor, are a kind of avirulent marine biomaterial with the effect of preventing and curing diseases.
Squalene is widely present in animal and plant body.In animal the highest with content in deepwater shark liver, mainly deep
In the armour shark in sea, spinule shark, edge kiss Tian Shi shark, New Zealand's crow shark liver, and the Squalene content of other sea, river shark is the lowest, general
All over less than 1.5%.In plant then the highest, as olive oil contains Squalene with content in olive oil, Amaranthus mangostanus L. seed oil and Petiolus Trachycarpi oil
150～700mg/100g, in Testa oryzae oil, its content is 332mg/100g.Deepwater shark is precious protection animal, adds it
Low rate of growth and low reproductive capacity, easy overfishing, cause the Animal resources containing Squalene to lack.Have now been found that containing Squalene
Plant resources accumulation is few, develops and has certain difficulty.Thus limit production and the use of Squalene.
Prior art has had open extraction Squalene from the plants such as Fructus Momordicae, tail alpine ash, glutinous rice scented tea, Nicotiana tabacum L..
Also someone reports that extracting Squalene from tobacco waste sees 201110058487.5；Therefore extracting Squalene in plant will be not
Obtain an important channel of Squalene.But in various plants, the content of Squalene is the highest, does not also have in prior art
Research shows to improve the report of Squalene content in plant by adding exogenous material in the plant growing stage.
Summary of the invention
It is an object of the invention to provide a kind of Terbinafine formulation and the application in improving Nicotiana tabacum L. Squalene content thereof.
In the growth course of Nicotiana tabacum L., add described Terbinafine formulation, it is possible to increase the content of Squalene in Nicotiana tabacum L..
The Terbinafine formulation that the present invention provides, percentage component consists of: terbinafine 10～20%；Three
(laureth-4) phosphate ester 5～8%；Surplus is ethanol.
Preferably, Terbinafine formulation, it is made up of following component in percentage by weight: terbinafine 20%；Three
(laureth-4) phosphate ester 8%；Surplus is ethanol.
Terbinafine is a kind of propylamine antifungal agent, has broad-spectrum antifungal effect.In prior art, pressed down by it
Squalene epoxidase on fungal cell membrane processed plays antifungic action.Inventors be surprised to learn that the growth course Nicotiana tabacum L.
In, add terbinafine, it is possible to increase the content of Squalene in Nicotiana tabacum L..
The invention provides Terbinafine formulation application in improving Nicotiana tabacum L. Squalene content.Preferably, described special ratio
The consumption of naphthalene sweet smell preparation is 30～50 grams/strain.More preferably 50 grams/strain.
The addition manner of terbinafine does not carry out special restriction, it is preferable that is preferably absorbed by plants and utilizes, described
Terbinafine is added by the form of spraying.
Preferably, foliage-spray after described Terbinafine formulation is dilute with water 500～1500 times.More preferably 1500
Times.
Preferably, described Terbinafine formulation is used once in the fast growing period of Nicotiana tabacum L. strain.
Preferably, described Terbinafine formulation is used once in 1 week after the pinching of Nicotiana tabacum L. strain.
It is highly preferred that described Terbinafine formulation is respectively used once in 1 week in the fast growing period of Nicotiana tabacum L. strain with after pinching.
Nicotiana tabacum L. of the present invention can be the Nicotiana tabacum L. of dry in the sun cigarette or flue-cured tobacco, it is preferable that described Nicotiana tabacum L. is cured tobacco leaf.
Nicotiana tabacum L. is in addition to the preparation being mainly used in medicated cigarette, and it can produce substantial amounts of such as offal, waste tobacco leaf, inapplicable Nicotiana tabacum L.
In waste product, usually can only be processed by modes such as degradeds, cause waste.Squalene is a kind of thing with economic worth
Matter, promotes the content of Squalene in Nicotiana tabacum L., the most also improves the economic worth of Nicotiana tabacum L., make offal, waste tobacco leaf etc.
The value of waste product is promoted.
Compared with prior art, there is advantages that
Inventor finds, in the growth course of Nicotiana tabacum L., adds a certain amount of Terbinafine formulation, it is possible to increase in Nicotiana tabacum L.
The content of Squalene, compared with blank group, after with the addition of terbinafine, the Squalene content in Nicotiana tabacum L. is obviously improved,
Improve the economic worth of Nicotiana tabacum L..
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further details.Embodiment is only used for explaining the present invention, and
Present disclosure is not limited in any form.
In embodiment, Terbinafine formulation used is autogamy, weighs a certain amount of terbinafine, is dissolved in ethanol, then adds
Enter a certain amount of surfactant three (laureth-4) phosphate ester, stir.
Wherein terbinafine is purchased from Jinan Hong Fangde Pharmaceutical Technology Co., Ltd；Three (laureth-4) phosphate ester is purchased from
Daylight chemistry trade (Shanghai) Co., Ltd..
Control treatment:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Randomly choose cigarette
Grass strain is handled as follows, and within 1 week, only sprays clear water in fast growing period after pinching；The quantity of each process is 30 strains.
Embodiment 1
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.
Being handled as follows every strain Nicotiana tabacum L. strain, the Terbinafine formulation spraying dilution 500 times in fast growing period is water-soluble
Liquid (before dilution, the consumption of Terbinafine formulation is 30g, following percetage by weight the component calculated forms: terbinafine 10%,
Three (laureth-4) phosphate ester 5%, surplus are ethanol).Randomly choose 30 strains.
After Nicotiana tabacum L. maturation, choosing 3 strain Nicotiana tabacum L.s, whole blades of every strain are cleaned, and blot surface moisture with gauze, in removing
Arteries and veins, 105 DEG C of 15min that complete, to dry to constant weight, pulverize, cross 40 mesh sieves for 60 DEG C, gained sample is used for measuring Squalene in Nicotiana tabacum L. and contains
Amount.
The mensuration of the absolute content of Squalene in Nicotiana tabacum L.
Weighing above-mentioned gained Nicotiana tabacum L. powder 20g filter paper parcel to load in apparatus,Soxhlet's, bath temperature is constant simultaneously is
75 DEG C, add 200mL petroleum ether (60～90 DEG C) and be heated at reflux 4h, be dried petroleum ether layer with anhydrous sodium sulfate, take petroleum ether and carry
Take liquid Rotary Evaporators to rotate to dry, obtain yellow leaf tobacco extract, be dissolved to 50mL with petroleum ether, utilize gas chromatogram, adopt
The absolute content (mg/Kg) of Squalene in Nicotiana tabacum L. is gone out with internal mark method determination.
GC conditions is as follows: HP-5MS capillary column (30m × 0.25mm × 0.25 μm), column temperature 150 DEG C, program liter
Temperature 15 DEG C/min to 290 DEG C, constant temperature 15min；Carrier gas is high-purity He；Inlet temperature 300 DEG C；Split ratio 20: 1；Solvent excises
1.5min；Sample size 0.5 μ L.
The mensuration of the relative amount of Squalene in flue gas
Reference literature (GC/MS method detects Squalene content [J] in 24 kinds of flue-cured tobaccos. guangdong agricultural science, and 2012,39
(3) tobacco heat desorption apparatus: 105-107.), is utilized to obtain the flue gas ligroin extraction of Nicotiana tabacum L. powder: Nicotiana tabacum L. powder is Nicotiana tabacum L.
Burning in thermal desorption device, the flue gas produced during burning is pumped into the cone equipped with petroleum ether sealed under the effect of vacuum pump
In shape bottle, the composition in flue gas is absorbed by petroleum ether.
Then the composition in the GC/MS flue gas to extracting is utilized to be analyzed measuring: to arrive according to Nist standard spectrum library searching
Qualitative results, determines its percentage composition according to mass spectrographic total ion current figure, and the workstation software carried according to mass spectrograph is returned
One changes mensuration, obtains the percentage contents (%) of Squalene in flue gas.
Its Mass Spectrometry Conditions is as follows: GC/MS interface temperature 290 DEG C；Ion source temperature 235 DEG C；Ionization mode EI source；Ionization electricity
Pressure 70eV；Mass scan range 30～500amu；SCAN (full scan) detects.
Test result is as shown in table 1 below.
Embodiment 2
Tobacco seedlings plantation and Squalene extract and embodiment 1 is shown in detection.
Every strain Nicotiana tabacum L. strain is handled as follows, within 1 week after tobbaco, sprays the Terbinafine formulation of dilution 1000 times
(before dilution, the consumption of Terbinafine formulation is 40g, following percetage by weight the component calculated forms: terbinafine 10%, three
(laureth-4) phosphate ester 8%, surplus are ethanol).Randomly choose 30 strains.
Test result is as shown in table 1 below.
Embodiment 3
Tobacco seedlings plantation and Squalene extract and embodiment 1 is shown in detection.
Every strain Nicotiana tabacum L. strain is handled as follows, within 1 week, respectively sprays dilution 1500 times in Nicotiana tabacum L. fast growing period after pinching
Terbinafine formulation (before dilution, the consumption of Terbinafine formulation is 50g, following percetage by weight the component calculated forms:
Terbinafine 10%, three (laureth-4) phosphate ester 8%, surplus are ethanol).Randomly choose 30 strains.
Test result is as shown in table 1 below.
Embodiment 4:
Tobacco seedlings plantation and Squalene extract and embodiment 1 is shown in detection.
Every strain Nicotiana tabacum L. strain is handled as follows, sprays the Terbinafine formulation (dilution of dilution 500 times in fast growing period
The consumption of front Terbinafine formulation is 30g, following percetage by weight the component calculated forms: terbinafine 20%, three (Laurel
Alcohol polyethers-4) phosphate ester 8%, surplus be ethanol).Randomly choose 30 strains.
Test result is as shown in table 1 below.
Embodiment 5:
Tobacco seedlings plantation and Squalene extract and embodiment 1 is shown in detection.
Every strain Nicotiana tabacum L. strain is handled as follows, within 1 week after tobbaco, sprays the Terbinafine formulation of dilution 1000 times
(before dilution, the consumption of Terbinafine formulation is 40g, following percetage by weight the component calculated forms: terbinafine 20%, three
(laureth-4) phosphate ester 8%, surplus are ethanol).Randomly choose 30 strains.
Test result is as shown in table 1 below.
Embodiment 6:
Tobacco seedlings plantation and Squalene extract and embodiment 1 is shown in detection.
Every strain Nicotiana tabacum L. strain is handled as follows, within 1 week, respectively sprays dilution 1500 times in Nicotiana tabacum L. fast growing period after pinching
Terbinafine formulation (before dilution, the consumption of Terbinafine formulation is 50g, following percetage by weight the component calculated forms:
Terbinafine 20%, three (laureth-4) phosphate ester 8%, surplus are ethanol).Randomly choose 30 strains.
Test result is as shown in table 1 below.
Table 1 Squalene test result
It can be seen that after within 1 week, respectively spraying 1 Terbinafine formulation in Nicotiana tabacum L. fast growing period with after pinching, in Nicotiana tabacum L.
Squalene content is significantly improved.
Claims (11)
1. a Terbinafine formulation, it is characterised in that percentage component consists of: terbinafine 10～20%；
Three (laureth-4) phosphate ester 5～8%；Surplus is ethanol.
Apply the most according to claim 1, it is characterised in that percentage component consists of: terbinafine 20%；
Three (laureth-4) phosphate ester 8%；Surplus is ethanol.
The Terbinafine formulation the most according to claim 1 or claim 2 application in improving Nicotiana tabacum L. Squalene content.
Apply the most according to claim 3, it is characterised in that the consumption of described Terbinafine formulation is 30～50 grams/strain.
Application the most according to claim 4, it is characterised in that the consumption of described Terbinafine formulation is 50 grams/strain.
Application the most according to claim 3, it is characterised in that described Terbinafine formulation is dilute with water 500～1500
Foliage-spray after times.
Application the most according to claim 6, it is characterised in that described Terbinafine formulation is 1500 times of posterior lobes of dilute with water
Face sprays.
Application the most according to claim 3, it is characterised in that described Terbinafine formulation is in the fast growing period of Nicotiana tabacum L. strain
Use once.
Application the most according to claim 3, it is characterised in that described Terbinafine formulation after the pinching of Nicotiana tabacum L. strain 1 week
Inside use once.
Application the most according to claim 3, it is characterised in that described Terbinafine formulation is at the fast-growth of Nicotiana tabacum L. strain
Phase respectively uses once in 1 week after pinching.
11. apply according to claim 3, it is characterised in that the consumption of described Terbinafine formulation is 50 grams/strain；Described
Terbinafine formulation is foliage-spray after dilute with water 1500 times；Described Terbinafine formulation Nicotiana tabacum L. strain fast growing period and
Respectively use once in 1 week after pinching.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610454678.6A CN106106449A (en) | 2016-06-21 | 2016-06-21 | Terbinafine formulation and the application in improving Nicotiana tabacum L. Squalene content thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610454678.6A CN106106449A (en) | 2016-06-21 | 2016-06-21 | Terbinafine formulation and the application in improving Nicotiana tabacum L. Squalene content thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106106449A true CN106106449A (en) | 2016-11-16 |
Family
ID=57471250
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610454678.6A Pending CN106106449A (en) | 2016-06-21 | 2016-06-21 | Terbinafine formulation and the application in improving Nicotiana tabacum L. Squalene content thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106106449A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111436428A (en) * | 2020-05-18 | 2020-07-24 | 兰州大学 | Application of naphthaline benzylamine antifungal drugs in prevention and treatment of agricultural diseases |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491206A (en) * | 2001-02-07 | 2004-04-21 | ��˹��ŵ�� | Malic acid addition salts of terbinafine |
| EP1532977A1 (en) * | 2002-06-28 | 2005-05-25 | Sato Pharmaceutical Co. Ltd. | Antifungal agents for external use |
| CN102161611A (en) * | 2011-03-11 | 2011-08-24 | 中国烟草总公司广东省公司 | Method for preparing squalene based on tobacco as raw material |
-
2016
- 2016-06-21 CN CN201610454678.6A patent/CN106106449A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491206A (en) * | 2001-02-07 | 2004-04-21 | ��˹��ŵ�� | Malic acid addition salts of terbinafine |
| EP1532977A1 (en) * | 2002-06-28 | 2005-05-25 | Sato Pharmaceutical Co. Ltd. | Antifungal agents for external use |
| CN102161611A (en) * | 2011-03-11 | 2011-08-24 | 中国烟草总公司广东省公司 | Method for preparing squalene based on tobacco as raw material |
Non-Patent Citations (1)
| Title |
|---|
| LAURENT F. WENTZINGER ET AL: "Inhibition of Squalene Synthase and Squalene Epoxidase in Tobacco Cells Triggers an Up-Regulation of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase", 《PLANT PHYSIOL.》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111436428A (en) * | 2020-05-18 | 2020-07-24 | 兰州大学 | Application of naphthaline benzylamine antifungal drugs in prevention and treatment of agricultural diseases |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Solovyev et al. | Selenium-rich mushrooms cultivation on a wheat straw substrate from seleniferous area in Punjab, India | |
| CN103833470B (en) | A kind of cultivation of glossy ganoderma culture medium that utilizes little seeds of a tung oil tree shell and little seeds of a tung oil tree seed coat to make | |
| CN101336644A (en) | Plant insecticide prepared by humulus and its preparing process horehound | |
| CN106106449A (en) | Terbinafine formulation and the application in improving Nicotiana tabacum L. Squalene content thereof | |
| CN107032904A (en) | With the plant source amino acid matrimony vine special foliar fertilizer for increasing production and preventing and treating pest and disease damage | |
| CN103416165B (en) | A kind of method of Rosemary harvest and the preparation method of Rosemary antioxidant extract | |
| Srinophakun et al. | Integrated research on Jatropha curcas plantation management | |
| CN107223503A (en) | Promote the method for rice root heavy metal cadmium accumulation and its rice plant of preparation using 5 amino-laevulic acids | |
| CN106070219A (en) | Seaweeds application in improving Nicotiana tabacum L. Squalene content | |
| CN106116749A (en) | Liquid fertilizer and the application in improving Nicotiana tabacum L. Squalene content thereof | |
| CN107821455B (en) | Traditional Chinese medicine preparation for health care and quality improvement of tobacco and use method thereof | |
| Harper et al. | Changes in Reduction and Assimilation of Nitrogen During the Growth Cycle of Winter Wheat 1 | |
| Mohammadreza et al. | Effects of different levels of nitrogen, phosphorus and potassium fertilizers on some agromorphological and biochemical traits of German chamomile (Matricaria chamomilla L.) | |
| CN106045629B (en) | It prints chinaberry fertilizer and its is improving the application in tobacco leaf squalene content | |
| CN107278654A (en) | Promote the method for paddy rice stem heavy metal cadmium accumulation and its rice plant of preparation using 24 Epibrassinolides | |
| CN109566031A (en) | A kind of Rumex madaio selenium-enriching cultivation method for applying selenium fertilizer according to soil selenium-containing amount | |
| Saadatfar et al. | Improving biochemical traits, oleo-gum yield and compositions of asafoetida (Ferula assa-foetida L.) essential oil using 24-epibrassinolide in Kerman natural habitats (Iran) | |
| CN106167425A (en) | Fertilizer composition and the application in improving Nicotiana tabacum L. Squalene content thereof | |
| CN108142988A (en) | A kind of preparation method of Fructus Monordicae extract for flavouring humectation | |
| CN105859364A (en) | Tea tree fruit dreg fertilizer and application thereof in improvement of tobacco leaf squalene content | |
| CN107646912A (en) | A kind of grape tree planting insecticide and preparation method thereof | |
| CN105859483A (en) | Fertilizer composition and application thereof to improving content of squalene in tobacco leaves | |
| CN106187401A (en) | Chelating fertilizer and the application in improving Nicotiana tabacum L. Squalene content thereof | |
| CN105859365A (en) | Compound fertilizer and application thereof to improving content of squalene in tobacco leaves | |
| CN106332916A (en) | Inhibitor for inhibiting sundry fungi in ganoderma lucidum generation material cultivation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161116 |
|
| RJ01 | Rejection of invention patent application after publication |