CN106116749A - Liquid fertilizer and the application in improving Nicotiana tabacum L. Squalene content thereof - Google Patents
Liquid fertilizer and the application in improving Nicotiana tabacum L. Squalene content thereof Download PDFInfo
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- CN106116749A CN106116749A CN201610454630.5A CN201610454630A CN106116749A CN 106116749 A CN106116749 A CN 106116749A CN 201610454630 A CN201610454630 A CN 201610454630A CN 106116749 A CN106116749 A CN 106116749A
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- China
- Prior art keywords
- liquid fertilizer
- nicotiana tabacum
- application
- strain
- described liquid
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
Abstract
The invention discloses a kind of liquid fertilizer and the application in improving Nicotiana tabacum L. Squalene content thereof.Fluid present invention micro-fertile percentage preferably constitutes as EDTA Zn 0.2～5%；Ammonium phosphomolybdate 0.5～10%；Ethanol 5～10%；Surplus is water.In the growth course of Nicotiana tabacum L., adding the liquid fertilizer of the present invention, the Squalene content in Nicotiana tabacum L. is obviously improved, and improves the economic worth of Nicotiana tabacum L..
Description
Technical field
The present invention relates to tobacco planting field, more particularly, to a kind of liquid fertilizer and at raising Nicotiana tabacum L. Squalene
Application in content.
Background technology
Squalene is a kind of lipid non-saponifiable matter, for a kind of many unsaturated olefins (squalene), is initially from shark
Liver oil in find, within 1914, be named as Squalene, its chemical name is 2,6,10,15,19,23-hexamethyl-2,6,
10,14,18,22-tetracosa carbon six alkene, belong to open chain triterpene, also known as Squalene, participate in human body cholesterol biosynthesis and can promote new
Old metabolism, has raising internal superoxide dismutase (SOD) activity, enhancing human body immunity ability, defying age, resisting fatigue, resists
The different physiological roles such as tumor, are a kind of avirulent marine biomaterial with the effect of preventing and curing diseases.
Squalene is widely present in animal and plant body.In animal the highest with content in deepwater shark liver, mainly deep
In the armour shark in sea, spinule shark, edge kiss Tian Shi shark, New Zealand's crow shark liver, and the Squalene content of other sea, river shark is the lowest, general
All over less than 1.5%.In plant then the highest, as olive oil contains Squalene with content in olive oil, Amaranthus mangostanus L. seed oil and Petiolus Trachycarpi oil
150～700mg/100g, in Testa oryzae oil, its content is 332mg/100g.Deepwater shark is precious protection animal, adds it
Low rate of growth and low reproductive capacity, easy overfishing, cause the Animal resources containing Squalene to lack.Have now been found that containing Squalene
Plant resources accumulation is few, develops and has certain difficulty.Thus limit production and the use of Squalene.
Prior art has had open extraction Squalene from the plants such as Fructus Momordicae, tail alpine ash, glutinous rice scented tea, Nicotiana tabacum L..
Also someone reports that extracting Squalene from tobacco waste sees 201110058487.5；Therefore extracting Squalene in plant will be not
Obtain an important channel of Squalene.In plant, Squalene is produced and in plant by specific biosynthesis pathway
Accumulation, but the content of Squalene is the highest in various plants, not there are some researches show in prior art can be by planting
Thing growth stage adds exogenous material, improves the report of Squalene content in plant.
Summary of the invention
It is an object of the invention to provide a kind of liquid fertilizer.In the growth course of Nicotiana tabacum L., add described liquid fertilizer, energy
Enough improve the content of Squalene in Nicotiana tabacum L..
Liquid fertilizer is a kind of novel fertilizer, and it, using organic nutrient substance as chelating agen, is tied with central metal atom
Close, form described liquid fertilizer.In the present invention, described liquid fertilizer comprises EDTA-Zn and ammonium phosphomolybdate.Described liquid is micro-
Fertilizer can be to be made up of EDTA-Zn and ammonium phosphomolybdate completely, or adds other compositions not affecting both material stability.
Preferably constituting as EDTA-Zn of fluid present invention micro-fertilizer；Ammonium phosphomolybdate；Second alcohol and water.
More preferably consisting of of the micro-fertilizer of fluid present invention: by weight percentage, its component consists of: EDTA-Zn0.2～
5%；Ammonium phosphomolybdate 0.5～10%；Ethanol 5～10%；Surplus is water.
Most preferably, EDTA-Zn is 1:1～5 with the mass ratio of ammonium phosphomolybdate, more preferably 1:2～3.
Ammonium phosphomolybdate can buy acquisition, or prepares with reference to following method: by 0.12mol (NH4)6Mo7O24·4H2O is dissolved in
In 2000ml distilled water, add 0.1mol H3PO4, and use dense HNO3Solution ph is adjusted to 1；By above-mentioned mixed liquor in room temperature
Lower stirring 1.5h, obtains yellow mercury oxide, is the most at room temperature aged 20h, filters, with the HNO of 1mol/L3Solution washs 3 times, then
With distilled water wash 1 time, finally it is dried, to obtain final product.
Invention also provides liquid fertilizer application in improving Nicotiana tabacum L. Squalene content.
Inventor finds in the growth course of Nicotiana tabacum L., the micro-fertilizer of adding liquid, it is possible to increase the content of Squalene in Nicotiana tabacum L..
Preferably, the consumption of described liquid fertilizer is every strain 30～50 grams.
Preferably, foliage-spray after described liquid fertilizer is dilute with water 500～2000 times.
Preferably, described liquid fertilizer is used once in the fast growing period of Nicotiana tabacum L. strain.
Preferably, described liquid fertilizer is used once after the pinching of Nicotiana tabacum L. strain for 1 week.
Preferably, described liquid fertilizer is respectively used once after pinching in the fast growing period of Nicotiana tabacum L. strain for 1 week.
Nicotiana tabacum L. of the present invention can be the Nicotiana tabacum L. of dry in the sun cigarette or flue-cured tobacco, it is preferable that described Nicotiana tabacum L. is cured tobacco leaf.
Nicotiana tabacum L. is in addition to the preparation being mainly used in medicated cigarette, and it can produce substantial amounts of such as offal, waste tobacco leaf, inapplicable Nicotiana tabacum L.
In waste product, usually can only be processed by modes such as degradeds, cause waste.Squalene is a kind of thing with economic worth
Matter, promotes the content of Squalene in Nicotiana tabacum L., the most also improves the economic worth of Nicotiana tabacum L., make offal, waste tobacco leaf etc.
The value of waste product is promoted.
Compared with prior art, there is advantages that
In the growth course of Nicotiana tabacum L., add liquid fertilizer of the present invention, it is possible to increase the content of Squalene in Nicotiana tabacum L.,
Compared with blank group, after with the addition of liquid fertilizer, the Squalene content in Nicotiana tabacum L. is obviously improved, and improves Nicotiana tabacum L.
Economic worth.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further details.Embodiment is only used for explaining the present invention, and
Present disclosure is not limited in any form.
In embodiment, the raw material EDTA-Zn (Shijiazhuang Jack Chemical Co., Ltd.) used by liquid fertilizer and ammonium heptamolybdate
(Anqing Yue Tongmu industry company limited), phosphoric acid, nitric acid (Guangzhou Chemical Reagent Factory) synthesis material are market and buy.
Used by embodiment, ammonium phosphomolybdate is prepared as follows: by 0.12mol (NH4)6Mo7O24·4H2O is dissolved in 2000ml
In distilled water, add 0.1mol H3PO4, and use dense HNO3Solution ph is adjusted to 1；Above-mentioned mixed liquor is stirred at room temperature
1.5h, obtains yellow mercury oxide, is the most at room temperature aged 20h, filters, with the HNO of 1mol/L3Solution washs 3 times, then with distilling
Water washs 1 time, is finally dried, to obtain final product.
Embodiment 1:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Liquid fertilizer group
Become: EDTA-Zn 0.2%；Ammonium phosphomolybdate 0.5%；Ethanol 5%；Surplus is water
Randomly choose Nicotiana tabacum L. strain to process by situations below: 1, every strain 50g liquid fertilizer, spray by dilution 500 times；2, every strain
50g liquid fertilizer, is sprayed by dilution 1000 times；3, every strain 50g liquid fertilizer, is sprayed by dilution 2000 times；4, clear water is only sprayed,
Without liquid fertilizer.The quantity that every kind of situation processes is 15 strains, at Nicotiana tabacum L. fast growing period or 1 week or fast-growth after pinching
Phase respectively executes 1 time in 1 week after pinching.
After Nicotiana tabacum L. maturation, every kind of situation selects 3 strains, and whole blades of every strain are cleaned, and blot surface moisture with gauze, in removing
Arteries and veins, 105 DEG C of 15min that complete, to dry to constant weight, pulverize, cross 40 mesh sieves for 60 DEG C, gained sample is used for measuring Squalene in Nicotiana tabacum L. and contains
Amount.
The mensuration of the absolute content of Squalene in Nicotiana tabacum L.
Weighing above-mentioned gained Nicotiana tabacum L. powder 20g filter paper parcel to load in apparatus,Soxhlet's, bath temperature is constant simultaneously is
75 DEG C, add 200mL petroleum ether (60～90 DEG C) and be heated at reflux 4h, be dried petroleum ether layer with anhydrous sodium sulfate, take petroleum ether and carry
Take liquid Rotary Evaporators to rotate to dry, obtain yellow leaf tobacco extract, be dissolved to 50mL with petroleum ether, utilize gas chromatogram, adopt
The absolute content (mg/Kg) of Squalene in Nicotiana tabacum L. is gone out with internal mark method determination.
GC conditions is as follows: HP-5MS capillary column (30m × 0.25mm × 0.25 μm), column temperature 150 DEG C, program liter
Temperature 15 DEG C/min to 290 DEG C, constant temperature 15min；Carrier gas is high-purity He；Inlet temperature 300 DEG C；Split ratio 20: 1；Solvent excises
1.5min；Sample size 0.5 μ L.
The mensuration of the relative amount of Squalene in flue gas
Reference literature (GC/MS method detects Squalene content [J] in 24 kinds of flue-cured tobaccos. guangdong agricultural science, and 2012,39
(3) tobacco heat desorption apparatus: 105-107.), is utilized to obtain the flue gas ligroin extraction of Nicotiana tabacum L. powder: Nicotiana tabacum L. powder is Nicotiana tabacum L.
Burning in thermal desorption device, the flue gas produced during burning is pumped into the cone equipped with petroleum ether sealed under the effect of vacuum pump
In shape bottle, the composition in flue gas is absorbed by petroleum ether.
Then the composition in the GC/MS flue gas to extracting is utilized to be analyzed measuring: to arrive according to Nist standard spectrum library searching
Qualitative results, determines its percentage composition according to mass spectrographic total ion current figure, and the workstation software carried according to mass spectrograph is returned
One changes mensuration, obtains the percentage contents (%) of Squalene in flue gas.
Its Mass Spectrometry Conditions is as follows: GC/MS interface temperature 290 DEG C；Ion source temperature 235 DEG C；Ionization mode EI source；Ionization electricity
Pressure 70eV；Mass scan range 30～500amu；SCAN (full scan) detects.
Test result is as shown in table 1 below:
Table 1
It can be seen that in the tobacco growing phase, after spraying liquid fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 2
Tobacco seedlings plantation and Squalene extract and embodiment 1 is shown in detection.
Liquid fertilizer consists of: EDTA-Zn 2%；Ammonium phosphomolybdate 6%；Ethanol 8%；Surplus is water.
Randomly choose Nicotiana tabacum L. strain to process by situations below: 1, every strain 30g liquid fertilizer, spray by dilution 500 times；2、
Every strain 30g liquid fertilizer, is sprayed by dilution 1000 times；3, every strain 30g liquid fertilizer, is sprayed by dilution 2000 times；4, only spray
Clear water, without liquid fertilizer.The quantity that every kind of situation processes was 15 strains, Nicotiana tabacum L. fast growing period or after pinching 1 week or soon
Fast-growing is long-term respectively executes 1 time in 1 week after pinching.
Test result is as shown in table 2 below:
Table 2
It can be seen that in the tobacco growing phase, spray liquid fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 3
Tobacco seedlings plantation and Squalene extract and embodiment 1 is shown in detection.
Liquid fertilizer consists of: EDTA-Zn 5%；Ammonium phosphomolybdate 10%；Ethanol 10%；Surplus is water.
Randomly choose Nicotiana tabacum L. strain to process by situations below: 1, every strain 40g liquid fertilizer, spray by dilution 500 times；2、
Every strain 40g liquid fertilizer, is sprayed by dilution 1000 times；3, every strain 40g liquid fertilizer, is sprayed by dilution 2000 times；4, only spray
Clear water, without liquid fertilizer.The quantity that every kind of situation processes was 15 strains, Nicotiana tabacum L. fast growing period or after pinching 1 week or soon
Fast-growing is long-term respectively executes 1 time in 1 week after pinching.
Test result is as shown in table 3 below:
Table 3
It can be seen that in the tobacco growing phase, spray liquid fertilizer, the Squalene content in Nicotiana tabacum L. is significantly improved.
Claims (12)
1. a liquid fertilizer, it is characterised in that its composition includes: EDTA-Zn, ammonium phosphomolybdate, second alcohol and water.
Liquid fertilizer the most according to claim 1, it is characterised in that percentage component consists of: EDTA-Zn
0.2～5%；Ammonium phosphomolybdate 0.5～10%；Ethanol 5～10%；Surplus is water.
Liquid fertilizer the most according to claim 2, it is characterised in that the mass ratio of EDTA-Zn and ammonium phosphomolybdate be 1:1～
5。
Liquid fertilizer the most according to claim 2, it is characterised in that percentage component consists of: EDTA-Zn
0.2%, ammonium phosphomolybdate 0.5%, ethanol 5%；Surplus is water.
5. according to described liquid fertilizer arbitrary in claim 1-4, it is characterised in that ammonium phosphomolybdate is prepared with the following method:
0.12mol (NH4) 6Mo7O24 4H2O is dissolved in 2000ml distilled water, adds 0.1mol H3PO4, and will with dense HNO3
Solution ph is adjusted to 1；Above-mentioned mixed liquor is stirred at room temperature 1.5h, obtains yellow mercury oxide, be the most at room temperature aged 20h,
Filter, wash 3 times with the HNO3 solution of 1mol/L, then with distilled water wash 1 time, be finally dried, to obtain final product.
6. arbitrary described liquid fertilizer application in improving Nicotiana tabacum L. Squalene content in claim 1-5.
Application the most according to claim 6, it is characterised in that the consumption of described liquid fertilizer is every strain 30～50 grams.
Application the most according to claim 6, it is characterised in that after described liquid fertilizer is dilute with water 500～2000 times
Foliage-spray.
Application the most according to claim 6, it is characterised in that described liquid fertilizer is used in the fast growing period of Nicotiana tabacum L. strain
Once.
Application the most according to claim 6, it is characterised in that described liquid fertilizer is used after the pinching of Nicotiana tabacum L. strain for 1 week
Once.
11. application according to claim 6, it is characterised in that described liquid fertilizer Nicotiana tabacum L. strain fast growing period and
Within after pinching 1 week, respectively use once.
12. application according to claim 5, it is characterised in that described liquid fertilizer Nicotiana tabacum L. strain fast growing period and
Within after pinching 1 week, respectively execute 1 time；Every strain 30g liquid fertilizer, is sprayed by dilution 1000 times；Described Nicotiana tabacum L. is dry in the sun cigarette or flue-cured tobacco
Nicotiana tabacum L..
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| CN201610454630.5A CN106116749A (en) | 2016-06-21 | 2016-06-21 | Liquid fertilizer and the application in improving Nicotiana tabacum L. Squalene content thereof |
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| CN201610454630.5A CN106116749A (en) | 2016-06-21 | 2016-06-21 | Liquid fertilizer and the application in improving Nicotiana tabacum L. Squalene content thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108329112A (en) * | 2018-03-30 | 2018-07-27 | 内蒙古拜克生物有限公司 | A kind of medicine fertilizer and preparation method thereof containing avermectin |
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2016
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|---|---|---|---|---|
| CN1064670A (en) * | 1991-10-24 | 1992-09-23 | 彭毓华 | Liquid fertilizer and preparation method thereof |
| US20020015741A1 (en) * | 2000-06-12 | 2002-02-07 | Bowen Ward Beryl | Method and composition for the accelerated in vivo removal of ethanol |
| CN101182256A (en) * | 2007-11-13 | 2008-05-21 | 韩瑜 | Nutrient balance agent and production technique therefor |
| CN105272456A (en) * | 2015-10-21 | 2016-01-27 | 四川省烟草公司攀枝花市公司（攀枝花市烟草公司） | Trace element fertilizer for tobacco as well as preparation method and application method of trace element fertilizer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108329112A (en) * | 2018-03-30 | 2018-07-27 | 内蒙古拜克生物有限公司 | A kind of medicine fertilizer and preparation method thereof containing avermectin |
| CN108329112B (en) * | 2018-03-30 | 2021-04-09 | 内蒙古拜克生物有限公司 | Pesticide fertilizer containing abamectin and preparation method thereof |
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