CN106085449A - Organophosphorus pesticide pollution soil remediation material - Google Patents
Organophosphorus pesticide pollution soil remediation material Download PDFInfo
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- CN106085449A CN106085449A CN201610442854.4A CN201610442854A CN106085449A CN 106085449 A CN106085449 A CN 106085449A CN 201610442854 A CN201610442854 A CN 201610442854A CN 106085449 A CN106085449 A CN 106085449A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The invention belongs to technical field of soil remediation, be specifically related to a kind of organophosphorus pesticide pollution soil remediation material.The soil remediation material of organophosphorus pesticide pollution, the component including following: chitin, Kaolin, chlorella, citric acid, conch meal, Flammulina velutiper (Fr.) Sing mushroom bran, corn cob, Testa oryzae, tea seed episperm, walnut shell, betelnut tree wood flour, palm wood flour, compound enzymic preparation, complex micro organism fungicide.Using the soil remediation material of the present invention, under conditions of the chemical solvent not introducing other, use gentle enzyme and microorganism fungus kind to process soil, do not produce secondary pollution, action condition is gentle.
Description
Technical field
The invention belongs to technical field of soil remediation, be specifically related to a kind of organophosphorus pesticide pollution soil remediation material.
Background technology
Organophosphorus pesticide is because high molecular polymer is the most oxidizable, decomposition, and also difficulty is degraded by microorganisms, can the most in the environment
It is detained, it may occur however that chemical change transforms into the secondary pollution that toxicity is higher, hazardness is bigger.Meanwhile, plant is remained in
Internal composition can make food Pesticides remaining quantity exceed sanitary standard by the enrichment of food chain.Organophosphorus pesticide
Human body can be entered through digestive tract, respiratory tract and complete skin and mucosa.Occupational pesticide intoxication is mainly caused by skin contamination.
The organophosphorus pesticide absorbed is distributed in each organ in vivo, and wherein maximum with liver content, in brain, content then depends on that pesticide is worn
The ability of blood brain barrier thoroughly.
Internal organophosphor first passes around oxidation and hydrolysis two ways bioconversion；Oxidation makes toxicity strengthen, as to sulfur
Phosphorus, under the mixed-function oxidase effect of liver smooth endoplasmic reticulum, is oxidized to the paraoxon that toxicity is bigger；Hydrolysis can make toxicity
Reducing, parathion, while oxidation, is hydrolyzed by phosphate and ineffective.Secondly, the metabolism after oxidized and hydrolysis is produced
Thing, the most again through glucuronic acid and sulphuric acid association reaction with urine ejection；Partial hydrolysate paranitrophenol or to nitro first
Phenol etc. are directly through urine ejection, without combined reaction.
Organophosphorus pesticide enters environment mainly three kinds of approach: one is that pesticide directly sprays in soil, is used for preventing and treating ground
Lower insect, go to cut weeds；Two is all kinds of pesticide prevented and treated disease pest and weed and be sprayed on crop, and at least a part of which has the agriculture of 56%
Medicine falls in the middle of soil；Three is that part pesticide fills the air in air when spraying and dusting use pesticide, and along with air-flow and wind
Migrate, spread to each corner of environment.
Restorative procedure to organophosphorus pesticide pollution soil at present, mainly has following several:
Peripheral doses method, chemical repair method, plant absorption method, plant-microorganism associating system is to organophosphorus pesticide
Conversion, specific as follows:
Peripheral doses method: air injection is mainly used in repairing by nonaqueous phase liquid in situ, particularly volatile organic matter
The saturated soil polluted and subsoil water.It is not enough that the sealed underground water system that biological restoration in the past utilizes often results in oxygen,
And bio-inert well provides substantial amounts of oxygen, thus promote biodegradation efficiency.
Plant absorption method: the organophosphorus pesticide in soil is absorbed by root system of plant, but root system of plant is to organic
The intensity that phosphorus pesticide absorbs is the highest, and it is repaired by organophosphorus pesticide contaminated soil, mainly relies on root exudates and enzyme
The effect such as the complexation producing organophosphorus pesticide and degraded carrys out degrading organic phosphor pesticides.Root system of plant can secrete sugar, alcohol, protein
Deng in nutrient substance to soil, for microbe survival；Root system of plant can also secrete the chemical substances such as organic acid, thus it is possible to vary soil
PH-value etc., the beneficially decomposition of organophosphorus pesticide.Research shows, has flourishing root system, the plant of root hair can promote root
Border microorganism species is to herbicide, the organic absorption of agrochemical and degraded.
The plant-microorganism associating system conversion to organophosphorus pesticide, mineralization and the mycorhiza of plant strengthening rhizosphere and
Being had certain relation by raw bacterium, mycorhiza has the double of microorganism and plant as the coalition of fungus and plant to the impact of soil
Weight characteristic, the ad hoc structure of mycorrhizal plants makes the absorption area of root system increase, can reduce the fluid resistance between plant and soil,
Promote that root system is to moisture and the absorption of nutrient and utilization.Secretions from plant roots can also stimulate the transformation of antibacterial, can increase
The microorganism mineralization to organophosphorus pesticide, additionally, plant is owing to having complicated root system, its huge root surface area
Also in place of being the boarding of microorganism, thus the growth for soil microorganism provides more existence place and environment, makes rhizosphere
The aerobic transformation of environment can be normally carried out.
Although above-mentioned phytoremediation technology has many good qualities, but still there is a lot of incomplete place, actual application
In have certain limitation, as in repair process, some plant has certain requirement to the condition such as soil, weather, is easily subject to
Restriction to self and extraneous factor.
Above-mentioned to the restorative procedure by organophosphorus pesticide contaminated soil, or having in soil can not be eliminated the most thoroughly
Machine phosphorus insecticide pollutant, or bring new pollutant, or it uses the restriction receiving environmental condition, there is various defect, because of
This, be required for above-mentioned present situation, grope a kind of remove organophosphorus pesticide effective and relatively mild do not produce new pollution
Soil-repairing agent.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of to by organophosphorus pesticide contaminated soil regulation effect
Good and action condition gentleness does not the most bring the renovation agent of new pollution, and this soil remediation material combines plant, enzyme and microorganism altogether
Same-action, in by the soil of organophosphorus pesticide, makes the organophosphorus pesticide in soil or absorption or is chelated or is degraded, reaching to control
The purpose of reason organophosphorus pesticide pollution soil.
The present invention is to be realized by following technical scheme:
The soil remediation material of organophosphorus pesticide pollution, including the component of following portions by weight:
Compound enzymic preparation includes laccase, peroxidase, aldolase；
The enzyme of laccase alive 6.8 × 105U/g；The enzyme of peroxidase alive 17.2 × 105U/g；The enzyme of aldolase lives 10.4 ×
105U/g；Laccase, peroxidase, the weight ratio of aldolase are 1-4:2-6:1-5；
Complex micro organism fungicide 0.005-0.048；
The active component of above-mentioned complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis bacterium
Powder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Above-mentioned thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve
Mycopowder, micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: (1-4): (1-5): (2-5): (1-6): (1-5): (2-
5)；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 2 × 108-9×109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 4 × 109-9×1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 5 × 108-9×109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 2.5 × 109-9×1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 3.5 × 109-9×1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 5.5 × 108-9×109cfu/g。
Preferably, the weight ratio of above-mentioned each mycopowder is:
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder,
Micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
Compound enzymic preparation is 0.008 part；
Complex micro organism fungicide 0.036 part.
It is furthermore preferred that the soil remediation material of the present invention includes the component of following portions by weight:
Compound enzymic preparation includes laccase, peroxidase, aldolase；
The enzyme of laccase alive 6.8 × 105U/g；Peroxidase 17.2 × 105U/g；Aldolase 10.4 × 105U/g；Laccase,
Peroxidase, the weight ratio of aldolase are 2:4:3；
Complex micro organism fungicide 0.036；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, bending
Pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder,
Micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 2 × 108-9×109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 4 × 109-9×1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 5 × 108-9×109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 2.5 × 109-9×1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 3.5 × 109-9×1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 5.5 × 108-9×109cfu/g。
Preferably, soil remediation material includes the component of following portions by weight:
Compound enzymic preparation is made up of laccase, peroxidase, aldolase, polyphenol oxidase；
The enzyme of laccase alive 6.8 × 105U/g；The enzyme of peroxidase alive 17.2 × 105U/g；The enzyme of aldolase lives 10.4 ×
105U/g, the enzyme work of polyphenol oxidase are 12.5 × 105U/g；Laccase, peroxidase, aldolase, the weight of polyphenol oxidase
Portion rate is 2:5:2:3；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, bending
Pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder,
Micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 8 × 109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 5 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
It is furthermore preferred that the soil remediation material of organophosphorus pesticide pollution, including the component of following portions by weight:
Mix with pedotheque, keep 48 hours；
Mycopowder of the present invention, enzyme are derived from commercially available；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Add complex micro organism fungicide 0.036 part, mix homogeneously；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, bending
Pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder,
Micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 8 × 109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 5 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 6 × 109cfu/g。
The beneficial effects of the present invention is, use the soil remediation material of the present invention, do not introducing other chemical solvent
Under conditions of, use gentle enzyme and microorganism fungus kind that soil is processed, do not bring new pollution, action condition temperature into
With, strain effectively degrades organophosphorus pesticide.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, in order to those skilled in the art knows more about
The present invention, but and it is not so limited the present invention.
Embodiment 1
Take by organochlorine pesticide contaminated soil sample, then join the ratio of soil-repairing agent 50 grams by every 1000 grams of pedotheques
Example fetches earth soil repair agent；
Following raw material is added: chitin, Kaolin, chlorella, citric acid, conch meal, golden mushroom in pedotheque
Bran, corn cob, Testa oryzae, tea seed episperm, walnut shell, betelnut tree wood flour, palm wood flour；
The parts by weight of each raw material are as follows:
Mix with pedotheque, keep 48 hours；
Mycopowder of the present invention, enzyme are derived from commercially available；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Add complex micro organism fungicide 0.036 part, mix homogeneously；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, bending
Pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder,
Micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 8 × 109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 5 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 6 × 109cfu/g；
Above living bacteria count is approximate number, as follows；
Being eventually adding compound enzymic preparation 0.008 part, mix homogeneously, compound enzymic preparation is by laccase, peroxidase, al
Enzyme, polyphenol oxidase are formed, and the enzyme of laccase is lived and is about 6.8 × 105U/g；The enzyme of peroxidase is lived and is about 17.2 × 105U/
g；The enzyme of aldolase is lived and is about 10.4 × 105U/g, the enzyme of polyphenol oxidase are lived and are about 12.5 × 105U/g；Laccase, peroxide
Enzyme, aldolase, the ratio of weight and number of polyphenol oxidase are 2:4:3:2；
This compound enzymic preparation first stirs in the water of 45 DEG C, is then sprayed in pedotheque, and by soil-like
Product stir；
Above " part " is parts by weight, and following example are same, if no special instructions.
Inventor has been again following contrast experiment about the application, specific as follows:
Comparative example 1
With not being both of embodiment 1, in comparative example 1 and be provided without compound enzymic preparation, remaining is identical；
Comparative example 2
With not being both of embodiment 1, in comparative example 1 and be provided without complex micro organism fungicide, remaining is identical；
Comparative example 3
With not being both of embodiment 1, complex micro organism fungicide is different, and the concrete complex micro organism fungicide used is as follows:
Bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, cement sand thunder
The weight ratio of Salmonella mycopowder is: 3:4:3:3:4；
Comparative example 4
With not being both of embodiment 1, the compound enzyme that compound enzymic preparation is laccase, peroxidase is formed, the enzyme of laccase
Work is 6.8 × 105U/g；The work of peroxidase enzyme is 17.2 × 105U/g；Laccase, the ratio of weight and number of peroxidase are 3:2.
Comparative example 5
As different from Example 1, being provided without complex micro organism fungicide and compound enzymic preparation, remaining is identical.At place
During reason soil, and after pedotheque mixing, keep 48 hours.
Pedotheque and renovation agent being placed in the container of a cuboid, pedotheque and renovation agent lay about 20-25
Centimetre is thick, by above-mentioned pedotheque and soil-repairing agent are fully mixed and keep by the above-mentioned time after, then holding
Trickle in device, keeps pedotheque and renovation agent to moisten；Drip washing in every 10 days is once；Aqueous solution passes through below container with filter screen
Outlet is drained.When 1 month, 2 months time, 3 months time survey the content of organophosphorus pesticide in soil respectively.Use gas chromatography
Measure the content of organophosphorus pesticide in pedotheque, the computing formula of organochlorine pesticide degradation rate:
Y=(M-W)/M × 100%
In formula, Y is organic phosphorus pesticide degradation rate, %；W is residue organophosphorus pesticide quality, g in pedotheque；M is soil
Original organophosphorus pesticide quality, g in sample.
Each comparative example is also adopted by above-mentioned processing mode, and result is as follows:
Organic phosphorus pesticide degradation rate in the pedotheque of table 1 embodiment 1 and comparative example 1-5
| 1 month | 2 months | 3 months | |
| Embodiment 1 | 21% | 47% | 78% |
| Comparative example 1 | 11% | 25% | 32% |
| Comparative example 2 | 9% | 17% | 26% |
| Comparative example 3 | 20% | 62% | 72% |
| Comparative example 4 | 18% | 61% | 75% |
| Comparative example 5 | 7% | 11% | 20% |
Embodiment 2
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Organophosphorus pesticide, is a difference in that the parts by weight etc. of soil remediation material are had any different；
Compound enzymic preparation is made up of laccase, peroxidase, aldolase；
The enzyme of laccase alive 6.8 × 105U/g；Peroxidase 17.2 × 105U/g；Aldolase 10.4 × 105U/g, polyphenol oxygen
The enzyme work changing enzyme is 12.5 × 105U/g；Laccase, peroxidase, nitro, the enzyme work of polyphenol oxidase are 12.5 × 105U/g is also
The ratio of weight and number of protoenzyme is 2:4:3:1；
Complex micro organism fungicide 0.005；
The active component of above-mentioned complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis bacterium
Powder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Above-mentioned thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve
Mycopowder, micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 1:1:2:1:1:2；
Above-mentioned thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve
In mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 8 × 109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 5 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
Embodiment 3
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Organophosphorus pesticide, is a difference in that the parts by weight etc. of soil remediation material are had any different；
Compound enzymic preparation is laccase, peroxidase, aldolase；
The enzyme of laccase alive 6.8 × 105The work of U/g, peroxidase enzyme is 17.2 × 105The work of U/g, aldolase enzyme is 10.4 ×
105U/g；The ratio of weight and number of laccase, peroxidase and aldolase is 1:2:1；
Complex micro organism fungicide 0.048；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, bending
Pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder,
Micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 4:5:5:6:5:5；
The living bacteria count of bacillus thuringiensis mycopowder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 5 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Embodiment 4
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Organophosphorus pesticide, is a difference in that the parts by weight etc. of soil remediation material are had any different；
Soil remediation material includes the component of following portions by weight:
Compound enzymic preparation is made up of laccase, peroxidase, aldolase, polyphenol oxidase；
The enzyme of laccase alive 6.8 × 105U/g；The enzyme of peroxidase alive 17.2 × 105U/g；The enzyme of aldolase lives 10.4 ×
105U/g, the enzyme work of polyphenol oxidase are 12.5 × 105U/g；Laccase, peroxidase, aldolase, the weight of polyphenol oxidase
Portion rate is 2:5:2:3；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, bending
Pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder,
Micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 8 × 109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 5 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
Embodiment 5
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Organophosphorus pesticide, is a difference in that the parts by weight etc. of soil remediation material are had any different；
Compound enzymic preparation includes laccase, peroxidase, aldolase；
The enzyme of laccase alive 6.8 × 105U/g；Peroxidase 17.2 × 105U/g；Aldolase 10.4 × 105U/g；Laccase,
Peroxidase, the ratio of weight and number of aldolase are 2:4:3；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, bending
Pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder,
Micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 5 × 109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 4 × 1010cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 6 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 6 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 7 × 109cfu/g。
After embodiment 2-5 all uses method same as in Example 1 to process, record the fall of organophosphorus pesticide in pedotheque
Solution rate result is as follows:
Claims (7)
1. the soil remediation material of organophosphorus pesticide pollution, including the component of following portions by weight:
Chitin 1-3 Kaolin 80-200
Chlorella 10-25 citric acid 0.5-2
Conch meal 2-9 Flammulina velutiper (Fr.) Sing mushroom bran 5-15
Corn cob 40-80 Testa oryzae 10-20
Tea seed episperm 5-15 walnut shell 5-20
Betelnut tree wood flour 20-80 palm wood flour 25-60
Compound enzymic preparation 0.001-0.02；
Described compound enzymic preparation includes laccase, peroxidase, aldolase；
The enzyme of described laccase alive 6.8 × 105U/g；The enzyme of peroxidase alive 17.2 × 105U/g ；The enzyme of aldolase alive 10.4
×105U/g；Laccase, peroxidase, the weight ratio of aldolase are 1-4:2-6:1-5；
Complex micro organism fungicide 0.005-0.048；
The active component of described complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, curved
Bent pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Described thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve bacterium
Powder, micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: (1-4): (1-5): (2-5): (1-6): (1-5): (2-5)；
The living bacteria count of described thiobacillus ferrooxidant mycopowder is 2 × 108-9×109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 4 × 109-9×1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 5 × 108-9×109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 2.5 × 109-9×1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 3.5 × 109-9×1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 5.5 × 108-9×109cfu/g。
2. the soil remediation material of organophosphorus pesticide pollution as claimed in claim 1, it is characterised in that the weight ratio of each mycopowder
For:
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder, microsphere
Bacterium mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4.
3. the soil remediation material of organophosphorus pesticide pollution as claimed in claim 1, it is characterised in that described compound enzyme system
Agent is 0.008 part.
4. the soil remediation material of organophosphorus pesticide pollution as claimed in claim 1, it is characterised in that described composite microbial
Thing microbial inoculum 0.036 part.
5. the soil remediation material of organophosphorus pesticide pollution as claimed in claim 1, it is characterised in that include following weight parts
The component of number:
Chitin 2 Kaolin 160
Chlorella 20 citric acid 1.5
Conch meal 6 Flammulina velutiper (Fr.) Sing mushroom bran 10
Corn cob 60 Testa oryzae 15
Tea seed episperm 10 walnut shell 15
Betelnut tree wood flour 60 palm wood flour 45
Compound enzymic preparation 0.008 complex micro organism fungicide 0.036；
Described compound enzymic preparation includes laccase, peroxidase, aldolase；
The enzyme of described laccase alive 6.8 × 105U/g；Peroxidase 17.2 × 105U/g ；Aldolase 10.4 × 105U/g；Paint
Enzyme, peroxidase, the ratio of weight and number of aldolase are 2:4:3；
The active component of described complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, curved
Bent pseudomonas mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Described thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve bacterium
Powder, micrococcus luteus mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 2 × 108-9×109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 4 × 109-9×1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 5 × 108-9×109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 2.5 × 109-9×1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 3.5 × 109-9×1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 5.5 × 108-9×109cfu/g。
6. the soil remediation material of organophosphorus pesticide pollution as claimed in claim 1, it is characterised in that include following weight parts
The component of number:
Soil remediation material includes the component of following portions by weight:
Chitin 2.5 Kaolin 160
Chlorella 18 citric acid 1.5
Conch meal 7 Flammulina velutiper (Fr.) Sing mushroom bran 12
Corn cob 60 Testa oryzae 16
Tea seed episperm 12 walnut shell 18
Betelnut tree wood flour 60 palm wood flour 50
Compound enzymic preparation 0.012；
Compound enzymic preparation is made up of laccase, peroxidase, aldolase, polyphenol oxidase；
The enzyme of laccase alive 6.8 × 105U/g；The enzyme of peroxidase alive 17.2 × 105U/g ；The enzyme of aldolase lives 10.4 ×
105U/g, the enzyme work of polyphenol oxidase are 12.5 × 105U/g；
Laccase, peroxidase, aldolase, the weight ratio of polyphenol oxidase are 2:5:2:3；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, the false list of bending
Born of the same parents' bacterium mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder, microsphere
Bacterium mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 8 × 109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 5 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
7. the soil remediation material of organophosphorus pesticide pollution as claimed in claim 1, it is characterised in that include following weight parts
The component of number:
Chitin 2 Kaolin 160
Chlorella 20 citric acid 1.5
Conch meal 6 Flammulina velutiper (Fr.) Sing mushroom bran 10
Corn cob 60 Testa oryzae 15
Tea seed episperm 10 walnut shell 15
Betelnut tree wood flour 60 palm wood flour 45；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, the false list of bending
Born of the same parents' bacterium mycopowder, flavobacterium breve mycopowder, micrococcus luteus mycopowder, serratia marcescens mycopowder are formed；
Thiobacillus ferrooxidant mycopowder, bacillus thuringiensis mycopowder, Pseudomonas geniculate mycopowder, flavobacterium breve mycopowder, microsphere
Bacterium mycopowder, the weight ratio of serratia marcescens mycopowder be: 3:3:4:3:3:4；
The living bacteria count of thiobacillus ferrooxidant mycopowder is 8 × 109cfu/g；
The living bacteria count of bacillus thuringiensis mycopowder is 6 × 1011cfu/g；
The living bacteria count of Pseudomonas geniculate mycopowder is 7 × 109cfu/g；
The living bacteria count of flavobacterium breve mycopowder is 5 × 1010cfu/g；
The living bacteria count of micrococcus luteus mycopowder is 5 × 1011cfu/g；
The living bacteria count of serratia marcescens mycopowder is 6 × 109cfu/g；
Compound enzymic preparation 0.008；
Compound enzymic preparation includes laccase, peroxidase, aldolase；
The enzyme of laccase alive 6.8 × 105U/g；Peroxidase 17.2 × 105U/g ；Aldolase 10.4 × 105U/g；Laccase, peroxide
Compound enzyme, the weight ratio of aldolase are 2:4:3.
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| CN201610442854.4A CN106085449A (en) | 2016-06-17 | 2016-06-17 | Organophosphorus pesticide pollution soil remediation material |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118987A (en) * | 2017-05-17 | 2017-09-01 | 江油市微生物技术应用研究院 | A kind of polyP bacteria agent and its application |
| CN107722992A (en) * | 2017-11-13 | 2018-02-23 | 郑州源冉生物技术有限公司 | A kind of repair materials of organic phosphor polluted soil and preparation method thereof |
| CN108018045A (en) * | 2017-12-22 | 2018-05-11 | 苏州萨瑟斯兰环境科技有限公司 | A kind of organophosphor contaminated soil remediation agent and preparation method thereof |
| CN108913156A (en) * | 2018-08-23 | 2018-11-30 | 广州市环境保护工程设计院有限公司 | A kind of pesticide contaminated soil renovation agent and preparation method thereof |
| CN111069265A (en) * | 2019-12-20 | 2020-04-28 | 纳琦绿能工程有限公司 | Soil remediation agent for removing organophosphorus pesticide in farmland soil |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1919807A (en) * | 2005-08-25 | 2007-02-28 | 北京市绿泉科技发展有限公司 | Seaweed organism fertilizer for soil-restored_and environment-improved and preparation method thereof |
| CN102154103A (en) * | 2010-12-24 | 2011-08-17 | 河南省科学院生物研究所有限责任公司 | Composite microbial inoculum for broad-spectrum degradation of organophosphorus insecticides and preparation method thereof |
| CN102505010A (en) * | 2011-10-21 | 2012-06-20 | 上海交通大学 | Preparation method of trichoderma multifunctional soil modifying agents |
| CN102911875A (en) * | 2012-04-27 | 2013-02-06 | 河南省科学院生物研究所有限责任公司 | Preparation method for multifunctional soil remediation microbial inoculum |
| CN104449753A (en) * | 2014-10-23 | 2015-03-25 | 江苏盖亚环境工程有限公司 | Compound soil remediator and preparation method thereof |
| CN104531157A (en) * | 2014-12-09 | 2015-04-22 | 江苏盖亚环境工程有限公司 | Soil remediation agent for removing organic phosphorus pollution, preparation method and applications thereof |
| CN104673778A (en) * | 2015-03-12 | 2015-06-03 | 中国海洋大学 | Method for purifying highly-cyclic PAHs contaminated soil |
| CN104694427A (en) * | 2015-02-12 | 2015-06-10 | 李丽萍 | Preparation process for microbial preparation for repairing nitrotoluene contaminated soil |
| CN104694428A (en) * | 2015-02-12 | 2015-06-10 | 何颖霞 | Microbial preparation for removing nitrotoluene in soil |
-
2016
- 2016-06-17 CN CN201610442854.4A patent/CN106085449A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1919807A (en) * | 2005-08-25 | 2007-02-28 | 北京市绿泉科技发展有限公司 | Seaweed organism fertilizer for soil-restored_and environment-improved and preparation method thereof |
| CN102154103A (en) * | 2010-12-24 | 2011-08-17 | 河南省科学院生物研究所有限责任公司 | Composite microbial inoculum for broad-spectrum degradation of organophosphorus insecticides and preparation method thereof |
| CN102505010A (en) * | 2011-10-21 | 2012-06-20 | 上海交通大学 | Preparation method of trichoderma multifunctional soil modifying agents |
| CN102911875A (en) * | 2012-04-27 | 2013-02-06 | 河南省科学院生物研究所有限责任公司 | Preparation method for multifunctional soil remediation microbial inoculum |
| CN104449753A (en) * | 2014-10-23 | 2015-03-25 | 江苏盖亚环境工程有限公司 | Compound soil remediator and preparation method thereof |
| CN104531157A (en) * | 2014-12-09 | 2015-04-22 | 江苏盖亚环境工程有限公司 | Soil remediation agent for removing organic phosphorus pollution, preparation method and applications thereof |
| CN104694427A (en) * | 2015-02-12 | 2015-06-10 | 李丽萍 | Preparation process for microbial preparation for repairing nitrotoluene contaminated soil |
| CN104694428A (en) * | 2015-02-12 | 2015-06-10 | 何颖霞 | Microbial preparation for removing nitrotoluene in soil |
| CN104673778A (en) * | 2015-03-12 | 2015-06-03 | 中国海洋大学 | Method for purifying highly-cyclic PAHs contaminated soil |
Non-Patent Citations (2)
| Title |
|---|
| 张甲耀等编著: "《环境微生物学》", 31 December 2008 * |
| 王友保编著: "《土壤污染与生态修复实验指导》", 30 June 2015 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107118987A (en) * | 2017-05-17 | 2017-09-01 | 江油市微生物技术应用研究院 | A kind of polyP bacteria agent and its application |
| CN107722992A (en) * | 2017-11-13 | 2018-02-23 | 郑州源冉生物技术有限公司 | A kind of repair materials of organic phosphor polluted soil and preparation method thereof |
| CN108018045A (en) * | 2017-12-22 | 2018-05-11 | 苏州萨瑟斯兰环境科技有限公司 | A kind of organophosphor contaminated soil remediation agent and preparation method thereof |
| CN108913156A (en) * | 2018-08-23 | 2018-11-30 | 广州市环境保护工程设计院有限公司 | A kind of pesticide contaminated soil renovation agent and preparation method thereof |
| CN111069265A (en) * | 2019-12-20 | 2020-04-28 | 纳琦绿能工程有限公司 | Soil remediation agent for removing organophosphorus pesticide in farmland soil |
| CN111202941A (en) * | 2019-12-27 | 2020-05-29 | 仲恺农业工程学院 | Method for degrading organophosphorus pesticide by using bacterial laccase |
| CN111202941B (en) * | 2019-12-27 | 2022-06-03 | 仲恺农业工程学院 | Degradation of organophosphorus pesticides by bacterial laccase |
| CN113729017A (en) * | 2021-09-10 | 2021-12-03 | 贵州蓝鼎民族医药发展有限公司 | Organic pesticide capable of restoring soil |
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