CN106001101A - Restoration agent for heavy metal contaminated soil - Google Patents
Restoration agent for heavy metal contaminated soil Download PDFInfo
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- CN106001101A CN106001101A CN201610438095.4A CN201610438095A CN106001101A CN 106001101 A CN106001101 A CN 106001101A CN 201610438095 A CN201610438095 A CN 201610438095A CN 106001101 A CN106001101 A CN 106001101A
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- mycopowder
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
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Abstract
The invention belongs to the technical field of soil restoration, and particularly relates to a restoration agent for heavy metal contaminated soil. The restoration agent for the heavy metal contaminated soil comprises chitosan, bentonite, dunaliella, citric acid, shell powder, needle mushroom residues, corncob, rice bran, tea seed hulls, dicranum scoparium, areca catechu wood chips, palm wood chips, a complex enzyme preparation and a complex microbial agent. By means of the soil restoration agent, under the condition that other chemical solvents are not introduced, the soil is treated by adopting moderate enzyme and microbial strains, new pollution is not caused, and the action conditions are moderate.
Description
Technical field
The invention belongs to technical field of soil remediation, be specifically related to a kind of heavy-metal contaminated soil renovation agent.
Background technology
Heavy metal described in terms of environmental pollution, is primarily referred to as hydrargyrum, arsenic, lead, chromium, cadmium etc., these heavy metal polluted soils
Earth, not only causes soil productivity to decline and make grain drop in production, and can be at plant and animal cylinder accumulation and enter food
Chain, or the respiratory tract of people is entered by contaminated air, there is strong interaction with protein, enzyme etc. after entering human body, make
They lose activity, it is also possible to accumulation health risk in some organs of human body.
Heavy metal pollution of soil main source has industrial pollution source, agricultural pollution source and transportation etc..Because of commercial production
The heavy metal pollution that aggravation with Mining Development and the growing material demand of the mankind the are brought gesture in increase year by year.
Heavy metal pollution of soil not only brings huge economic loss because directly contributing grain drop in production, and through water, big
The medium such as gas, plant or food chain are finally detrimental to health.The most this pollution have chronicity, disguise and
The feature of irreversibility, therefore, is a stubborn problem to heavy metal pollution of soil reparation.
Following method is mainly used to administer in the soil by heavy metal pollution at present:
1 engineering measure:
Soil moved in improve the original method, soil replacement method, deep ploughing are digged, and this is the administering method of the traditional and classical heavy metal pollution of soil of comparison,
Do not limited by soil environment and condition.By these measures, the content of heavy metal in soil can be reduced, reduce a huge sum of money
Belong to the murder by poisoning that soil_plant_atmosphere continuum is produced, so that agricultural product reach food hygienic standard.Soil moved in improve the original method is at contaminated soil
Upper covering uncontamination upper soll layer or mixing, make pollutant levels reduce or reduce contacting of pollutant and root system of plant, thus
Reach to alleviate the purpose of harm.The way of new uncontamination soil is changed after partly or entirely being removed by table soil, referred to as soil replacement method,
It was verified that soil replacement method is the effective way administering heavy metals in farmland severe contamination.But the method can not solve
The originally heavy metal pollution problem of soil.
Solidification, stabilization method, be by physicochemical method, by the soil of heavy metal pollution by a certain percentage with solid
Agent mixes, and makes Zinc fractions have the firming body of some strength, chemical stability and low-permeability through ripening, thus reduces weight
Metal migration infiltration in the environment and biological effectiveness.But the destructible Soil structure of the method and the productivity, and once
Destroying, soil is difficult to restore to the original state, and the method is also the method cured the symptoms, not the disease, and the heavy metal after cured and stabilisation is still
Exist in soil, soil and plant are still had potential threat.
2 chemical harnessing measures
Leaching method, soil eluviation method is to utilize leacheate that the heavy metal in solid phase of soil is transferred in liquid phase of soil, then
The soil remediation method that waste water rich in heavy metal is recycled further.More for the leacheate of washed off soil, including
Inorganic solution abluent, organic acid and salt abluent, chelating agen and surfactant etc., the method easily produces new dirt
Dye.
Using modifying agent, chemical redemption is exactly input modifying agent in soil, by the absorption of heavy metal, oxidation also
Former, antagonism, to reduce the biological effectiveness of heavy metal.Conventional modifying agent has Calx, zeolite, calcium carbonate, phosphate, silicon
Hydrochlorate and the organic substance of promotion reduction.The method is a kind of in-situ remediation method, and heavy metal still remains in soil, easily
Reactivation harm plant, its potential threat does not eliminate.
3 biological restoration measures
Phytoremediation, its mechanism is mainly by the absorption of certain plants heavy metal element, accumulates and convert, reaches to subtract
The purpose of light and heavy metals contaminated soil, compared with traditional restorative procedure, phytoremediation has green, environmental protection, economic dispatch advantage,
Plant is removed the heavy metal in soil and mainly relies on plant extraction effect, root system filtration, phytovolatilization effect and plant
Immobilization role.
Microorganism remediation, soil microorganism includes the free microorganism relevant to plant root, symbiosis rhizosphere bacteria, mycorhiza
Fungus, microorganism gradually forms the population of some heavy metal resistances in Heavy-metal Polluted Environment, and they pass through biologic detoxication
Heavy metal produces resistance, it is possible to survive in metallic pollution environment.
Animal is repaired, and utilizes some lower animal such as Lumbricus in soil can absorb the characteristic of heavy metal, to a certain degree
Heavy metal ratio in upper reduction contaminated soil, reaches the purpose of animal restoration of soil polluted by heavy metal.
4 Agro-ecology reclamation activitiess
In recent years, a series of Agro-ecology reclamation activities is gradually applied to be repaired by heavy-metal contaminated soil, agriculture
Industry restoration of the ecosystem is based on phytoremediation, by the control of soil environment and two aspects of plant improves reparation effect
Rate, in terms of soil environment, is increase soil fertility by application of organic fertilizers, weakens the toxicity of heavy metal in soil, and it is right to reduce
The murder by poisoning of plant, or the biological effectiveness of heavy metal is improved by organic fertilizer, it is beneficial to the absorption of rehabilitation plant, improves and repair
Efficiency.In terms of plant, by plant cultivation and domestication, strengthen patience and the cumulative percentage of plant heavy metal, improve plant
Remediation efficiency.It addition, by regulating the ecologies such as such as soil moisture, soil, pH, soil redox status and gas epidemic disaster
The factor, utilizes ecological means to be controlled surrounding medium, to weaken the murder by poisoning of heavy metal on plants.
Above-mentioned to the restorative procedure by heavy-metal contaminated soil, or the huge sum of money in soil can not be eliminated the most thoroughly
Belong to, or bring new pollutant, there is various defect, accordingly, it would be desirable to for above-mentioned present situation, grope one and go removing heavy metals to imitate
The best and relatively mild do not produce new contaminated soil renovation agent.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of to good by heavy-metal contaminated soil regulation effect and
Action condition gentleness does not the most bring the renovation agent of new pollution, and this soil-repairing agent combines plant, enzyme and microorganism and jointly acts on
In contaminated by heavy metals soil, make the heavy metal in soil or absorption or be chelated or be degraded, reaching to administer heavy metal-polluted
The purpose of dye soil.
The present invention is to be realized by following technical scheme:
By the soil-repairing agent of heavy metal pollution, including the component of following portions by weight:
Compound enzymic preparation includes laccase, thioesterase, phytase；
The enzyme of laccase alive 5.4 × 105U/g；The enzyme work of thioesterase is 18.3 × 105U/g；The enzyme of phytase is lived
It is 8.9 × 105U/g；The ratio of weight and number of laccase, thioesterase and phytase is 1-4:2-6:1-5；
Complex micro organism fungicide 0.005-0.048；
The active component of above-mentioned complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, resistance to
Salt yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Above-mentioned pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium
Mycopowder, sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: (1-4): (1-5): (2-5): (1-6): (1-5):
(2-5)；
Above-mentioned pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium
In mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is 1 × 108-9×1011cfu/g。
Salt-toler ant yeast is methamidophos, embodiment included below all with, if no special instructions；
Preferably, the weight ratio of above-mentioned each mycopowder is:
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
Sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 3:3:4:3:3:4；
Compound enzymic preparation is 0.008 part；
Complex micro organism fungicide 0.036 part.
It is furthermore preferred that the soil-repairing agent of the present invention includes the component of following portions by weight:
Compound enzymic preparation includes laccase, thioesterase, phytase；
The enzyme of laccase alive 5.4 × 105U/g；The enzyme work of thioesterase is 18.3 × 105U/g；The enzyme work of phytase is 8.9 ×
105U/g；The ratio of weight and number of laccase, thioesterase and phytase is 2:4:3；
Complex micro organism fungicide 0.036；
The active component of complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt tolerant yeast
Bacterium mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
Sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 3:3:4:3:3:4；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
In sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is 1 × 108-9×1011cfu/g。
By the soil-repairing agent of heavy metal pollution, including the component of following portions by weight:
Dunaliella salina is to shine the algae powder after dry doubling is pulverized, and its water content is 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 4%；
Bent tail moss is also for shining the powder after dry doubling is pulverized, and its water content is 3%；
Tea seed episperm is to shine the powder after dry doubling is pulverized, and its water content is 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Add complex micro organism fungicide 0.036 part, mix homogeneously, keep 72 hours；
The active component of complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt tolerant yeast
Bacterium mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
Sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 3:3:4:3:3:4；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
In sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is each about 2 × 1010cfu/g；
Compound enzymic preparation 0.008 part, compound enzymic preparation is made up of α-amylase, laccase, thioesterase, phytase；
α-amylase, laccase, thioesterase, the ratio of weight and number of phytase are 2:3:2:4；
The enzyme work of α-amylase is 6.8 × 105U/g；The enzyme work of laccase is 5.4 × 105U/g；The enzyme work of thioesterase is 18.3
×105U/g；The enzyme work of phytase is 8.9 × 105U/g。
The beneficial effects of the present invention is, use the soil-repairing agent of the present invention, do not introducing other chemical solvent
Under the conditions of, using gentle enzyme and microorganism fungus kind that soil is processed, do not bring new pollution into, action condition is gentle,
Strain is not only degraded or has adsorbed heavy metal, and the soil condition improvement for soil has obvious effect.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, in order to those skilled in the art knows more about
The present invention, but and it is not so limited the present invention.
Embodiment 1
Take the pedotheque by heavy metal pollution, after testing this Lead In Soil content 380mg/kg；Chromium content 282mg/kg；
Mercury content 0.85mg/kg, cadmium 0.78mg/kg；
The ratio joining soil-repairing agent 50 grams in every 1000 grams of pedotheques again fetches earth soil repair agent；
Following raw material is added: chitosan, bentonite, Dunaliella salina, citric acid, conch meal, Flammulina velutiper (Fr.) Sing in pedotheque
Mushroom bran, corn cob, Testa oryzae, tea seed episperm, bent tail moss, betelnut tree wood flour, palm wood flour；
The parts by weight of each raw material are as follows:
Mix with pedotheque, keep 48 hours；
Chitosan, enzyme and microbial germ powder used in the present invention are derived from commercially available；
Dunaliella salina is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Bent tail moss is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Tea seed episperm is to shine the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Add complex micro organism fungicide 0.036 part, mix homogeneously, keep 72 hours；
The active component of complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt tolerant yeast
Bacterium mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
Sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 3:3:4:3:3:4；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
In sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is each about 2 × 1010cfu/g；
Be eventually adding compound enzymic preparation 0.008 part, mix homogeneously, compound enzymic preparation by α-amylase, laccase, thioesterase,
Phytase is formed；α-amylase, laccase, thioesterase, the ratio of weight and number of phytase are 2:3:2:4.
This compound enzymic preparation first stirs in the water of 45 DEG C, is then sprayed in pedotheque, and by soil-like
Product stir, and keep 96 hours；
The enzyme work of α-amylase is 6.8 × 105U/g；The enzyme work of laccase is 5.4 × 105U/g；The enzyme work of thioesterase is 18.3
×105U/g；The enzyme work of phytase is 8.9 × 105U/g；
Above " part " is parts by weight, and following example are same, if no special instructions.
Inventor has been again following contrast experiment about the application, specific as follows:
Comparative example 1
With not being both of embodiment 1, in comparative example 1 and be provided without compound enzymic preparation, remaining is identical；
Comparative example 2
With not being both of embodiment 1, in comparative example 1 and be provided without complex micro organism fungicide, remaining is identical；
Comparative example 3
With not being both of embodiment 1, complex micro organism fungicide is different, and the concrete complex micro organism fungicide used is as follows:
Pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder,
The weight ratio of Li Trichoderma mycopowder is: 3:4:3:3:4；
Comparative example 4
With not being both of embodiment 1, the compound enzyme that compound enzymic preparation is laccase, thioesterase is formed, the enzyme work of laccase is
5.4×105U/g；The work of thioesterase enzyme is 18.3 × 105U/g；Laccase, the ratio of weight and number of thioesterase are 3:2.
Comparative example 5
As different from Example 1, being provided without complex micro organism fungicide and compound enzymic preparation, remaining is identical.At place
During reason soil, and after pedotheque mixing, keep 48 hours.
Pedotheque and renovation agent being placed in the container of a cuboid, pedotheque and renovation agent lay about 20-25
Centimetre, by above-mentioned pedotheque and soil-repairing agent are fully mixed and keep by the above-mentioned time after, then at container
Middle addition spray liquid, this spray liquid is slightly acidic solution, specifically adds the acetic acid leacheate drip washing that concentration is 0.5-2%, often
The addition of secondary leacheate is pedotheque and 2-3 times of renovation agent gross weight；Drip washing in every 10 days is once；Leacheate passes through container
Lower section is drained with the outlet of filter screen.When 1 month, 2 months time, 3 months time, 6 months time survey each heavy metal in soil respectively
Content；Each comparative example is also adopted by above-mentioned processing mode, and (following embodiment is all adopted with the aforedescribed process, as said without feature
Bright) result is as follows:
When 1 month, using inductively coupled plasma emission spectrography to measure the content of heavy metal in soil, result is such as
Under:
| Lead mg/kg | Chromium mg/kg | Hydrargyrum mg/kg | Cadmium mg/kg | |
| Embodiment 1 | 362 | 272 | 0.76 | 0.68 |
| Comparative example 1 | 373 | 278 | 0.82 | 0.73 |
| Comparative example 2 | 376 | 280 | 0.84 | 0.76 |
| Comparative example 3 | 364 | 275 | 0.78 | 0.70 |
| Comparative example 4 | 366 | 274 | 0.76 | 0.69 |
| Comparative example 5 | 378 | 280 | 0.84 | 0.77 |
When 2 months, using inductively coupled plasma emission spectrography to measure the content of heavy metal in soil, result is such as
Under:
| Lead mg/kg | Chromium mg/kg | Hydrargyrum mg/kg | Cadmium mg/kg | |
| Embodiment 1 | 340 | 248 | 0.64 | 0.62 |
| Comparative example 1 | 361 | 269 | 0.74 | 0.65 |
| Comparative example 2 | 370 | 275 | 0.80 | 0.73 |
| Comparative example 3 | 350 | 253 | 0.69 | 0.65 |
| Comparative example 4 | 347 | 250 | 0.66 | 0.64 |
| Comparative example 5 | 377 | 279 | 0.84 | 0.75 |
When 3 months, using inductively coupled plasma emission spectrography to measure the content of heavy metal in soil, result is such as
Under:
| Lead mg/kg | Chromium mg/kg | Hydrargyrum mg/kg | Cadmium mg/kg | |
| Embodiment 1 | 316 | 220 | 0.47 | 0.50 |
| Comparative example 1 | 347 | 254 | 0.64 | 0.58 |
| Comparative example 2 | 362 | 273 | 0.75 | 0.68 |
| Comparative example 3 | 322 | 228 | 0.52 | 0.59 |
| Comparative example 4 | 321 | 225 | 0.52 | 0.57 |
| Comparative example 5 | 374 | 276 | 0.82 | 0.74 |
When 6 months, using inductively coupled plasma emission spectrography to measure the content of heavy metal in soil, result is such as
Under:
| Lead mg/kg | Chromium mg/kg | Hydrargyrum mg/kg | Cadmium mg/kg | |
| Embodiment 1 | 235 | 144 | 0.27 | 0.26 |
| Comparative example 1 | 306 | 210 | 0.45 | 0.38 |
| Comparative example 2 | 352 | 259 | 0.62 | 0.57 |
| Comparative example 3 | 278 | 168 | 0.37 | 0.36 |
| Comparative example 4 | 267 | 164 | 0.32 | 0.35 |
| Comparative example 5 | 375 | 269 | 0.80 | 0.71 |
Above data show, the soil-repairing agent of the present invention is by acting on by heavy-metal contaminated soil, by soil
In heavy metal become the state of easy dissolution, taken away by acid leacheate, and then reduce the content of heavy metal in soil, implement
The content of the example 1 heavy metal in the experiment soil of six months is below " standard of soil environment quality " secondary standard.
Embodiment 2
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Heavy metal, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Compound enzymic preparation is made up of laccase, thioesterase, phytase；
The enzyme of laccase alive 5.4 × 105U/g, the enzyme work of thioesterase are 18.3 × 105U/g, the enzyme work of phytase is 8.9 ×
105U/g；The ratio of weight and number of laccase, thioesterase and phytase is 2:4:3；
Complex micro organism fungicide 0.005；
The active component of above-mentioned complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, resistance to
Salt yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Above-mentioned pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium
Mycopowder, sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 1:1:2:1:1:2；
Above-mentioned pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium
In mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is each about 2 × 108cfu/g；
Dunaliella salina is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Bent tail moss is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
After using method same as in Example 1 to process, inductively coupled plasma emission spectrography is used to measure soil
The content of middle heavy metal, result is as follows:
| Lead mg/kg | Chromium mg/kg | Hydrargyrum mg/kg | Cadmium mg/kg | |
| 1 month | 364 | 274 | 0.77 | 0.69 |
| 2 months | 343 | 250 | 0.66 | 0.63 |
| 3 months | 320 | 227 | 0.48 | 0.52 |
| 6 months | 246 | 150 | 0.28 | 0.27 |
Embodiment 3
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Heavy metal, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Compound enzymic preparation is laccase, thioesterase, phytase；
The enzyme of laccase alive 5.4 × 105U/g, the enzyme work of thioesterase are 18.3 × 105U/g, the enzyme work of phytase is 8.9 ×
105U/g；The ratio of weight and number of laccase, thioesterase and phytase is 1:2:1；
Complex micro organism fungicide 0.048；
The active component of complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt tolerant yeast
Bacterium mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
Sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 4:5:5:6:5:5；
Above-mentioned pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium
In mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is each about 9 × 109cfu/g。
Dunaliella salina is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Bent tail moss is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
After using method same as in Example 1 to process, inductively coupled plasma emission spectrography is used to measure soil
The content of middle heavy metal, result is as follows:
| Lead mg/kg | Chromium mg/kg | Hydrargyrum mg/kg | Cadmium mg/kg | |
| 1 month | 361 | 273 | 0.76 | 0.68 |
| 2 months | 341 | 245 | 0.62 | 0.63 |
| 3 months | 315 | 221 | 0.46 | 0.51 |
| 6 months | 236 | 145 | 0.26 | 0.25 |
Embodiment 4
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Heavy metal, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Soil-repairing agent includes the component of following portions by weight:
Compound enzymic preparation is made up of α-amylase, laccase, thioesterase, phytase；
The enzyme work of α-amylase is 6.8 × 105U/g；The enzyme of laccase alive 5.4 × 105U/g；The enzyme work of thioesterase is 18.3 ×
105U/g；The enzyme work of phytase is 8.9 × 105U/g；α-amylase, laccase, thioesterase, the ratio of weight and number of phytase are 2:3:
2:4；
Complex micro organism fungicide 0.036；
The active component of complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt tolerant yeast
Bacterium mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
Sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 3:3:4:3:3:4；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder,
In sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is each about 6 × 109cfu/g；
Dunaliella salina is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Bent tail moss is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
After using method same as in Example 1 to process, inductively coupled plasma emission spectrography is used to measure soil
The content of middle heavy metal, result is as follows:
| Lead mg/kg | Chromium mg/kg | Hydrargyrum mg/kg | Cadmium mg/kg | |
| 1 month | 360 | 271 | 0.75 | 0.68 |
| 2 months | 339 | 246 | 0.64 | 0.61 |
| 3 months | 311 | 219 | 0.47 | 0.46 |
| 6 months | 231 | 143 | 0.24 | 0.25 |
Claims (6)
1. by the soil-repairing agent of heavy metal pollution, including the component of following portions by weight:
Chitosan 1-3 bentonite 80-200
Dunaliella salina 10-25 citric acid 0.5-2
Conch meal 2-9 Flammulina velutiper (Fr.) Sing mushroom bran 5-15
Corn cob 40-80 Testa oryzae 10-20
Tea seed episperm 5-15 song tail moss 5-20
Betelnut tree wood flour 20-80 palm wood flour 25-60
Compound enzymic preparation 0.001-0.02；
Described compound enzymic preparation includes laccase, thioesterase, phytase；
The enzyme of described laccase alive 5.4 × 105U/g；The enzyme work of thioesterase is 18.3 × 105U/g ；The enzyme of phytase lives 8.9 ×
105U/g；The ratio of weight and number of laccase, thioesterase and phytase is 1-4:2-6:1-5；
Complex micro organism fungicide 0.005-0.048；
The active component of described complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt tolerant ferment
Female bacterium mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Described pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium bacterium
Powder, sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: (1-4): (1-5): (2-5): (1-6): (1-5):
(2-5)；
Described pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium bacterium
In powder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is 1 × 108-9×1011cfu/ g。
2. as claimed in claim 1 by the soil-repairing agent of heavy metal pollution, it is characterised in that the weight ratio of each mycopowder is:
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulphuric acid
Salt reducing bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 3:3:4:3:3:4.
3. as claimed in claim 1 by the soil-repairing agent of heavy metal pollution, it is characterised in that described compound enzymic preparation is
0.008 part.
4. as claimed in claim 1 by the soil-repairing agent of heavy metal pollution, it is characterised in that described composite microbial bacteria
Agent 0.036 part.
5. as claimed in claim 1 by the soil-repairing agent of heavy metal pollution, it is characterised in that include following portions by weight
Component:
Chitosan 2 bentonite 160
Dunaliella salina 20 citric acid 1.5
Conch meal 6 Flammulina velutiper (Fr.) Sing mushroom bran 10
Corn cob 60 Testa oryzae 15
The bent tail moss 15 of tea seed episperm 10
Betelnut tree wood flour 60 palm wood flour 45
Compound enzymic preparation 0.008 complex micro organism fungicide 0.036；
Described compound enzymic preparation includes laccase, thioesterase, phytase；
The enzyme of described laccase alive 5.4 × 105U/g；The enzyme of thioesterase alive 18.3 × 105U/g ；The enzyme of phytase lives 8.9 ×
105U/g；The ratio of weight and number of laccase, thioesterase and phytase is 2:4:3；
The active component of described complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt tolerant ferment
Female bacterium mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Described pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium bacterium
Powder, sulfate reducting bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 3:3:4:3:3:4；
Described pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium bacterium
In powder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is 1 × 108-9×1011cfu/ g。
6. as claimed in claim 1 by the soil-repairing agent of heavy metal pollution, it is characterised in that include following portions by weight
Component:
Chitosan 2 bentonite 160
Dunaliella salina 20 citric acid 1.5
Conch meal 6 Flammulina velutiper (Fr.) Sing mushroom bran 10
Corn cob 60 Testa oryzae 15
The bent tail moss 15 of tea seed episperm 10
Betelnut tree wood flour 60 palm wood flour 45；
Dunaliella salina is to shine the algae powder after dry doubling is pulverized, and its water content is 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 4%；
Bent tail moss is also for shining the powder after dry doubling is pulverized, and its water content is 3%；
Tea seed episperm is to shine the powder after dry doubling is pulverized, and its water content is 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast bacterium
Powder, schizosaccharomyces pombe bacterium mycopowder, sulfate reducting bacteria mycopowder, Li Trichoderma mycopowder are formed；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulphuric acid
Salt reducing bacteria mycopowder, the weight ratio of Li Trichoderma mycopowder be: 3:3:4:3:3:4；
Pseudomonas putida mycopowder, pseudomonas fluorescens mycopowder, salt-toler ant yeast mycopowder, schizosaccharomyces pombe bacterium mycopowder, sulphuric acid
In salt reducing bacteria mycopowder, Li Trichoderma mycopowder, living bacteria count is 2 × 1010cfu/g；
Compound enzymic preparation 0.008 part, compound enzymic preparation is made up of α-amylase, laccase, thioesterase, phytase；
α-amylase, laccase, thioesterase, the ratio of weight and number of phytase are 2:3:2:4；
The enzyme work of α-amylase is 6.8 × 105U/g ；The enzyme work of laccase is 5.4 × 105U/g；The enzyme work of thioesterase is 18.3 ×
105U/g ；The enzyme work of phytase is 8.9 × 105U/g。
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| CN107254322A (en) * | 2017-08-18 | 2017-10-17 | 中山市绿浪助剂有限公司 | A kind of soil-repairing agent containing microbial bacterial agent |
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| CN107254322A (en) * | 2017-08-18 | 2017-10-17 | 中山市绿浪助剂有限公司 | A kind of soil-repairing agent containing microbial bacterial agent |
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| CN107892921A (en) * | 2017-11-28 | 2018-04-10 | 湖南本农环境科技有限公司 | A kind of heavy metal polluted soil of farmland passivator and its preparation method and application |
| CN108329083A (en) * | 2017-12-11 | 2018-07-27 | 河南科技学院 | A kind of environmental protection heavy metal-polluted soil renovation agent |
| CN108440077A (en) * | 2018-01-16 | 2018-08-24 | 刘肖俊 | A kind of novel ecological afforestation soil remediation inorganic agent |
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| CN109622593A (en) * | 2018-12-06 | 2019-04-16 | 钦州学院 | A kind of biological renovation method of cadmium pollution soil |
| CN109705879A (en) * | 2019-02-15 | 2019-05-03 | 河南丰汇农业技术有限公司 | A kind of renovation agent of heavy-metal contaminated soil |
| CN110564425A (en) * | 2019-09-16 | 2019-12-13 | 赵龙 | heavy metal contaminated soil remediation agent and preparation method and use method thereof |
| CN112170475A (en) * | 2020-09-08 | 2021-01-05 | 吉林省林业科学研究院 | Heavy metal contaminated soil remediation agent and remediation method |
| CN113228872A (en) * | 2021-05-28 | 2021-08-10 | 西南科技大学 | Method for repairing acidic black rock stratum slope |
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