CN106085448A - Oil-polluted soils renovation agent - Google Patents
Oil-polluted soils renovation agent Download PDFInfo
- Publication number
- CN106085448A CN106085448A CN201610438118.1A CN201610438118A CN106085448A CN 106085448 A CN106085448 A CN 106085448A CN 201610438118 A CN201610438118 A CN 201610438118A CN 106085448 A CN106085448 A CN 106085448A
- Authority
- CN
- China
- Prior art keywords
- mycopowder
- rhodococcus
- enzyme
- bacterium
- nitroreductase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
Abstract
The invention belongs to technical field of soil remediation, be specifically related to a kind of oil-polluted soils renovation agent.Petroleum-polluted soil-repairing agent, the component including following: chitosan, bentonite, chlorella, citric acid, conch meal, Flammulina velutiper (Fr.) Sing mushroom bran, corn cob, Testa oryzae, tea seed episperm, walnut shell, betelnut tree wood flour, palm wood flour, compound enzymic preparation, complex micro organism fungicide.Using the soil-repairing agent of the present invention, under conditions of the chemical solvent not introducing other, use gentle enzyme and microorganism fungus kind to process soil, do not bring new pollution into, action condition is gentle.
Description
Technical field
The invention belongs to technical field of soil remediation, be specifically related to a kind of oil-polluted soils renovation agent.
Background technology
The composition of the Hydrocarbon in oil is sufficiently complex, and comes from and also do not differed by the petroleum components of oil well
Sample.Wherein contain ring-type Hydrocarbon, ring-type have saturated and unsaturation point, nuclear carbon number is the most each different,
The Hydrocarbon of chain also have saturated and unsaturation point, chain have long also have short；It is miscellaneous for also having many compositions in oil
Cycle compound.
Oil-polluted soils is a kind of typical organic contamination soil, divides with site relation by Pollutant Treatment form, can
It is divided into in-situ immobilization, field repair and showering.Common based technique for in-situ remediation has soil vapor extraction, bio-ventilation, former
Position chemical oxidation, two-way extracting etc..Based technique for in-situ remediation drilling well to be coordinated is aspirated.SVE is mainly for the height in unsaturated district
Volatile contaminant, such as the light hydrocarbon in oil product, gasoline etc., and for poor diesel oil, fuel oil and the kerosene of volatility
Deng clearance the highest, be more suitable for use BV.For the component of more difficult removal, then need to use oxidant applying to improve removal
Rate.Two-way extracting can to the subsoil water that saturated and unsaturated district pollutes, oil product freely mutually the most gentle identical time process, but
The gas extracted and liquid need individual processing, expend substantial contribution, it is possible to combine with other based technique for in-situ remediation
Improve treatment effeciency, common field repair technology such as soil cultivating, oil-polluted soils can be turned over ventilation with original place, and
It is properly added nutrient substance, mineral to promote biodegradation, is about repair time in a good condition 6 months to 2 years,
Operating cost is relatively low, but floor space is relatively big, high to initial organic concentration or have oil-polluted soils inapplicable, and ploughs
Tail gas after ventilating needs through processing and could be vented.Common showering technology such as biological compost is to be excavated by contaminated soil
After deliver to appointed place and carry out subsequent treatment, almost the removal of petroleum component all of to Polluted area is all effective, and can pass through
The system design closed controls waste gas discharge, but quantities is relatively big, expends fund many.Additionally, by oil-polluted soils and coal dust
Mixing, adds appropriate dispersant and combustion adjuvant molding, a kind of cost-effective method of also can yet be regarded as solid fuel.
The composition of the selection of oil-polluted soils recovery technique and treatment effect and petroleum pollution and character, oil pollution
Thing transition in soil are closely related with conditions such as soil environments.Over nearly 30 years, external relevant oil-polluted soils biology is repaiied
The research of recovering technology presents persistently overheating trend, from laboratory to in-situ immobilization on the spot, is separated to gene from degradation bacteria
The application of engineering, constantly expands in scale and in theory and deeply.Based technique for in-situ remediation is relatively easy, and cost is relatively low,
But it is big to there is environmental effect, time-consumingly grows, take effect slowly and easily make the shortcomings such as pollutant diffusion.Showering technology can be by strengthening
Degradation condition improves treatment effeciency, but is intended to additionally transport contaminated soil, takies certain place and installation ventilation installation equal
Processing cost can be increased.The kind of oil residues and residual quantity have been largely fixed the technology difficulty that oil-polluted soils is repaired
Degree, method choice, fund input and persistent period.
The at present reparation to oil-polluted soils mainly uses following several method:
Heat treating process: the method is to be heated by contaminated soil so that hydrocarbon decomposes or volatilization, from
And reach to purify the purpose of soil, conventional heating means mainly have infrared ray, microwave and radio frequency, pipeline to inject steam etc.,
The method is due to relatively costly, therefore, is only applicable to little area and the more serious situation of damage ratio.
Isolation method: utilize impervious material, such as cement, clay, slabstone, plastics etc., contaminated soil on the spot with uncontaiminated soil
Earth or water body separately, to reduce the diffusion of pollutant, the method be mainly used in seriously polluted, easily spread, and pollutant exist
Situation about can decompose in a period of time.Owing to some component in oil is not easily decomposed, therefore, this method is for removing in soil
Oil pollution effect poor.
Soil replacement method: be broadly divided into dig, soil removal and replacement and soil moved in improve the original these three method, these methods are all to utilize fresh not getting dirty
The soil of dye is replaced or partial replacement contaminated soil, reaches to dilute the purpose of pollutant, and it removes the machine of pollutant
Reason mainly make use of the environmental carrying capacity of soil, and wherein digging is to utilize the unpolluted soil of deep layer to dilute, soil removal and replacement be then by
The transfer of contaminated soil changes unpolluted soil into, and soil moved in improve the original is then to be directly added into unpolluted soil in original soil, from
And reducing the purpose of pollutant levels level, it is clear that this method is mainly used for processing the feelings that contaminated area is less
Condition.
Other physical method also has burning method, air stripping method etc., and physical method removes the mechanism of oil pollution mainly
The transfer of pollutant, does not fundamentally solve pollution problem, and the most this kind of method is unworthy advocating, but happens suddenly for some
Emergency, still has necessity of application.
Chemical method:
Extraction: be to utilize organic solvent to the petroleum substance extracting in soil, then the material in organic facies entered
Row separates, and reclaims petroleum substance, and this method is suitable for the situation that area is little and oil pollution concentration is higher.Due to organic molten
The use of agent so that the cost of reparation is significantly raised, and the response rate of organic solvent is the lowest, therefore governs the method
Development.Having the extraction that a kind of comparison is special, be also called floatation, it make use of the feature of oily substance hydrophobic, uses water as
Extractant extracts soil so that the petroleum substance in soil and water stratification, thus reaches to remove the purpose of oil pollutant,
This method can reach 65-80% for particle diameter petroleum substance clearance in the soil particle of 75-8300 micron, due to
This method need not extra organic solvent, is therefore considered there is wide development space.
Soil washing method: utilize the cleaning mixture that surfactant and water mix, soaks broken contaminated soil, thus
In aqueous phase, it is enriched the surfactant containing pollutant, the soil after washing is backfilled to original place.Conventional surface
Activating agent is the addition product of the branched-chain alcoho containing 8-15 carbon and 2-8 ethylene oxide unit.In order to prevent oil and cleaning mixture shape
Become emulsion, it is necessary to the amount of limiting surface activating agent is less than 0.05%.Soil washing method can effectively remove the oil in soil
Pollute, but easily cause secondary pollution problems.
Chemical oxidization method: utilize the oxidisability of chemical substance that the petroleum hydrocarbon component in soil is removed, conventional
Oxidant has chlorine dioxide, ozone, hydrogen peroxide, potassium permanganate etc., wherein thinks that chlorine dioxide cost is low, and to petroleum hydrocarbon
There is higher treatment effeciency.It is generally same with the oil pollution removed in subsoil water that chemical oxidization method removes the oil pollution in soil
Shi Jinhang, can be combined with air stripping method and carry out.
Other method: in addition to three kinds of above-mentioned methods, photocatalytic method, CSP method are also the most commonly used methods, and light is urged
The main thought of change method is to find suitable photocatalyst so that the decomposition rate of petroleum hydrocarbon is accelerated by light；CSP rule is to utilize
The material of carbon containing, such as coal, coke etc., as adsorbate, in conjunction with techniques such as floatations so that petroleum hydrocarbon separates with soil.
Biological method
Bioremediation technology is the emerging technology of a cleaning ambient of development in recent years, and it is to utilize specific micro-life
The vital movement of thing, animal, plant etc. removes the pollutant in environment, this technology has that treatment conditions are few, low cost, without two
The remarkable advantage of secondary pollution, increasingly comes into one's own.The Liaohe Oil Fields such as Li Peijun, Guo Shuhai are object of study, have studied difference
The oil-polluted soils of type, the restorative procedure of water body, and carried out relevant bacteria species identifying and dynamically analyzing；Mao Lihua etc. adopt
By the method for bio-ventilation compost, in laboratory, the reparation to oil-polluted soils is simulated research, at 40 days
Reason, the soil oil clearance containing oil 5% has reached more than 45%；Han Huilong etc. use fungus-bacterial micro-organism preparation pair
Saturated hydrocarbons, aromatic hydrocarbons, asphalt colloid and non-hydrocarbon compound are respectively provided with preferable degradation capability, by plantation Semen Tritici aestivi, are further characterized by
The feasibility in reparation petroleum hydrocarbon contaminated arable land and good application prospect.
The above-mentioned restorative procedure to petroleum-polluted soil, or the oil in soil can not be eliminated the most thoroughly,
Or bring new pollutant, there is various defect, accordingly, it would be desirable to for above-mentioned present situation, grope a kind of removal Petroleum concentration
Thing effective and relatively mild do not produce new contaminated soil renovation agent.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of good to petroleum-polluted soil regulation effect and make
The most do not bring the renovation agent of new pollution by mild condition, this soil-repairing agent combines plant, enzyme and microorganism and jointly acts on
By the soil of oil pollution, make the oil in soil be degraded, reach to administer the purpose of oil-polluted soils.
The present invention is to be realized by following technical scheme:
Petroleum-polluted soil-repairing agent, including the component of following portions by weight:
Compound enzymic preparation includes aldehyde dehydrogenase, catalase, nitroreductase；
The enzyme of aldehyde dehydrogenase alive 5.4 × 105U/g；Catalatic enzyme alive 18.3 × 105U/g；The enzyme of nitroreductase
Alive 8.9 × 105U/g；Aldehyde dehydrogenase, catalase, the ratio of weight and number of nitroreductase are 1-4:2-6:1-5；
Complex micro organism fungicide 0.005-0.048；
The active component of above-mentioned complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white
Candidiasis mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Above-mentioned lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens bacterium
Powder, thiobacillus ferrooxidant mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder be: (1-4): (1-5): (2-5): (1-6): (1-5):
(2-5)；
Above-mentioned lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens bacterium
In powder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is 1 × 108-9×109cfu/g。
Preferably, the weight ratio of above-mentioned each mycopowder is:
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Change ferrous Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder is: 3:3:4:3:3:4；Thiobacillus ferrooxidant mycopowder is de-
Sulfur
Compound enzymic preparation is 0.008 part；
Complex micro organism fungicide 0.036 part.
It is furthermore preferred that the soil-repairing agent of the present invention includes the component of following portions by weight:
Compound enzymic preparation includes aldehyde dehydrogenase, catalase, nitroreductase；
The enzyme of aldehyde dehydrogenase alive 5.4 × 105U/g；Catalase 18.3 × 105U/g；Nitroreductase 8.9 × 105U/
g；Aldehyde dehydrogenase, catalase, the ratio of weight and number of nitroreductase are 2:4:3；
Complex micro organism fungicide 0.036；
The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk ferment
Female bacterium mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Change ferrous Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder is: 3:3:4:3:3:4；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Changing in ferrous Thiobacillus mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is 1 × 108-9×109cfu/g。
As the improvement of the present invention, petroleum-polluted soil-repairing agent includes the component of following portions by weight:
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is 2-5%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 2-6%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is 1-5%；
Betelnut tree wood flour, palm wood flour be dry be 2-5% to moisture after and the bits shape thing pulverized；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk ferment
Female bacterium mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Change ferrous Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder is: 3:3:4:3:3:4；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Changing in ferrous Thiobacillus mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is each about 2 × 109cfu/g；
Compound enzymic preparation 0.008 part, compound enzymic preparation is by aldehyde dehydrogenase, catalase, nitroreductase, thioesterase institute
Composition；
The enzyme of aldehyde dehydrogenase is lived and is about 5.4 × 105U/g；Catalatic enzyme is lived and is about 18.3 × 105U/g；Nitro is also
The enzyme of protoenzyme is lived and is about 8.9 × 105U/g, the enzyme of thioesterase are lived and are about 11.3 × 105U/g；
Aldehyde dehydrogenase, catalase, nitroreductase, the ratio of weight and number of thioesterase are 2:4:3:2.
Another as the present invention improves, petroleum-polluted soil-repairing agent, including the component of following portions by weight:
Compound enzymic preparation is made up of aldehyde dehydrogenase, catalase, nitroreductase, Enone reductase；
The enzyme of aldehyde dehydrogenase alive 5.4 × 105U/g；Catalatic enzyme alive 18.3 × 105U/g；The enzyme of nitroreductase
Alive 8.9 × 105U/g, the enzyme work of Enone reductase are 11.3 × 105U/g；Aldehyde dehydrogenase, catalase, nitroreductase, alkene
The ratio of weight and number of ketoreductase is 2:5:2:4；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk ferment
Female bacterium mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Change ferrous Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder is: 3:3:4:3:3:4；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Changing in ferrous Thiobacillus mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
The beneficial effects of the present invention is, use the soil-repairing agent of the present invention, do not introducing other chemical solvent
Under the conditions of, use gentle enzyme and microorganism fungus kind that soil is processed, action condition is gentle, simple to operate, process effect
The best, do not produce secondary pollution, do not destroy soil environment.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, in order to those skilled in the art knows more about
The present invention, but and it is not so limited the present invention.
Embodiment 1
Petroleum degradation rate measures
Use gravimetric method, pedotheque is placed in 75 DEG C of baking ovens be dried 12 hours to constant weight, pulverize and sieve, accurately weigh
10g pedotheque, wraps with qualitative filter paper in the apparatus,Soxhlet's being placed in band plug ground, accurately weighs the weight of 50ml round-bottomed flask
Amount (being accurate to 0.0001g), pours the chloroform of about 1/2 volume into, hot dipping in the water-bath of 90 DEG C, until chloroform color in siphon
For colourless.Again by boiling flask being soaked with the chloroform of oil in 90 DEG C of distillations, afterwards flask is placed in 100 DEG C of baking ovens drying,
Claiming gross weight, the weight of increase is residual petroleum quality in pedotheque.The computing formula of petroleum degradation rate:
Y=(M-W)/M × 100%
In formula, Y is petroleum degradation rate, %；W is residual petroleum quality, g in pedotheque；M is original stone in pedotheque
Oil quality, g.
Near Changqing oilfields, take pedotheque, then the ratio joining soil-repairing agent 50 grams in every 1000 grams of pedotheques takes
Soil-repairing agent；
Following raw material is added: chitosan, bentonite, chlorella, citric acid, conch meal, golden mushroom in pedotheque
Bran, corn cob, Testa oryzae, tea seed episperm, walnut shell, betelnut tree wood flour, palm wood flour；
The parts by weight of each raw material are as follows:
Mix with pedotheque, keep 48 hours；
Mycopowder of the present invention, enzyme are derived from commercially available；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Add complex micro organism fungicide 0.036 part, mix homogeneously；
The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk ferment
Female bacterium mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Change ferrous Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder is: 3:3:4:3:3:4；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Changing in ferrous Thiobacillus mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is each about 2 × 109cfu/g；Lock shadow yeast bacterium bacterium
Powder is asymmetric lock shadow yeast bacterium mycopowder；
Being eventually adding compound enzymic preparation 0.008 part, mix homogeneously, compound enzymic preparation is by aldehyde dehydrogenase, catalase, nitre
Base reductase, thioesterase are formed, and the enzyme of aldehyde dehydrogenase is lived and is about 5.4 × 105U/g；Catalatic enzyme is lived and is about 18.3
×105U/g；The enzyme of nitroreductase is lived and is about 8.9 × 105U/g, the enzyme of thioesterase are lived and are about 11.3 × 105U/g；Aldehyde dehydrogenation
Enzyme, catalase, nitroreductase, the ratio of weight and number of thioesterase are 2:4:3:2；
This compound enzymic preparation first stirs in the water of 45 DEG C, is then sprayed in pedotheque, and by soil-like
Product stir；
Above " part " is parts by weight, and following example are same, if no special instructions.
Inventor has been again following contrast experiment about the application, specific as follows:
Comparative example 1
With not being both of embodiment 1, in comparative example 1 and be provided without compound enzymic preparation, remaining is identical；
Comparative example 2
With not being both of embodiment 1, in comparative example 1 and be provided without complex micro organism fungicide, remaining is identical；
Comparative example 3
With not being both of embodiment 1, complex micro organism fungicide is different, and the concrete complex micro organism fungicide used is as follows:
Bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder,
The weight ratio of Rhodococcus chlorophenolicus mycopowder is: 3:4:3:3:4；
Comparative example 4
With not being both of embodiment 1, the compound enzyme that compound enzymic preparation is aldehyde dehydrogenase, catalase is formed, aldehyde takes off
The enzyme work of hydrogen enzyme is 5.4 × 105U/g；The work of catalase enzyme is 18.3 × 105U/g；Aldehyde dehydrogenase, catalatic weight
Portion rate is 3:2.
Comparative example 5
As different from Example 1, being provided without complex micro organism fungicide and compound enzymic preparation, remaining is identical.At place
During reason soil, and after pedotheque mixing, keep 48 hours.
Pedotheque and renovation agent being placed in the container of a cuboid, pedotheque and renovation agent lay about 20-25
Centimetre is thick, by above-mentioned pedotheque and soil-repairing agent are fully mixed and keep by the above-mentioned time after, then holding
Trickle in device, keeps pedotheque and renovation agent to moisten；Drip washing in every 10 days is once；Aqueous solution passes through below container with filter screen
Outlet is drained.When 1 month, 2 months time, 3 months time survey the content of oil in soil respectively；Each comparative example is also adopted by above-mentioned
Processing mode, result is as follows:
The oil in pedotheque in embodiment 1 and comparative example 1-5 is respectively at the degradation rate of following time:
| 1 month | 2 months | 3 months | |
| Embodiment 1 | 26% | 65% | 81% |
| Comparative example 1 | 18% | 42% | 55% |
| Comparative example 2 | 12% | 23% | 36% |
| Comparative example 3 | 24% | 60% | 74% |
| Comparative example 4 | 22% | 56% | 71% |
| Comparative example 5 | 9% | 14% | 20% |
Embodiment 2
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Oil, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Compound enzymic preparation is made up of aldehyde dehydrogenase, catalase, nitroreductase；
The enzyme of aldehyde dehydrogenase alive 5.4 × 105U/g；Catalase 18.3 × 105U/g；Nitroreductase 8.9 × 105U/
G, the enzyme work of thioesterase are 11.3 × 105U/g；Aldehyde dehydrogenase, catalase, the ratio of weight and number of nitroreductase are 2:4:
3:1；
Complex micro organism fungicide 0.005；
The active component of above-mentioned complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white
Candidiasis mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Above-mentioned lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens bacterium
Powder, thiobacillus ferrooxidant mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder be: 1:1:2:1:1:2；
Above-mentioned lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens bacterium
In powder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is each about 2 × 108cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
Embodiment 3
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Oil, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Compound enzymic preparation is aldehyde dehydrogenase, catalase, nitroreductase；
The enzyme of aldehyde dehydrogenase alive 5.4 × 105The work of U/g, catalase enzyme is 18.3 × 105U/g, nitroreductase enzyme are lived
It is 8.9 × 105U/g；The ratio of weight and number of aldehyde dehydrogenase, catalase and nitroreductase is 1:2:1；
Complex micro organism fungicide 0.048；
The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk ferment
Female bacterium mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Change ferrous Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder is: 4:5:5:6:5:5；
Above-mentioned lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens bacterium
In powder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is each about 9 × 109cfu/g。
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Embodiment 4
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Oil, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Following raw material is added: chitosan, bentonite, chlorella, citric acid, conch meal, golden mushroom in pedotheque
Bran, corn cob, Testa oryzae, tea seed episperm, walnut shell, betelnut tree wood flour, palm wood flour；
The parts by weight of each raw material are as follows:
Mix with pedotheque, keep 48 hours；
Mycopowder of the present invention, enzyme are derived from commercially available；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized；
Add complex micro organism fungicide 0.036 part, mix homogeneously；
The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk ferment
Female bacterium mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Change ferrous Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder is: 3:3:4:3:3:4；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Changing in ferrous Thiobacillus mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is each about 2 × 109cfu/g；
Being eventually adding compound enzymic preparation 0.008 part, mix homogeneously, compound enzymic preparation is by aldehyde dehydrogenase, catalase, nitre
Base reductase is formed；
The enzyme of aldehyde dehydrogenase is lived and is about 5.4 × 105U/g；Catalatic enzyme is lived and is about 18.3 × 105U/g；Nitro is also
The enzyme of protoenzyme is lived and is about 8.9 × 105U/g；Aldehyde dehydrogenase, catalase, the ratio of weight and number of nitroreductase are 2:4:3；
This compound enzymic preparation first stirs in the water of 45 DEG C, is then sprayed in pedotheque, and by soil-like
Product stir.
Embodiment 5
Identical with the pedotheque in embodiment 1, use the method identical with embodiment 1 to remove in pedotheque
Oil, is a difference in that the parts by weight etc. of soil-repairing agent are had any different；
Soil-repairing agent includes the component of following portions by weight:
Compound enzymic preparation is made up of aldehyde dehydrogenase, catalase, nitroreductase, Enone reductase；
The enzyme of aldehyde dehydrogenase alive 5.4 × 105U/g；Catalatic enzyme alive 18.3 × 105U/g；The enzyme of nitroreductase
Alive 8.9 × 105U/g, the enzyme work of thioesterase are 11.3 × 105U/g；
Aldehyde dehydrogenase, catalase, nitroreductase, the ratio of weight and number of Enone reductase are 2:5:2:4；
Complex micro organism fungicide 0.036 part；
The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk ferment
Female bacterium mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Change ferrous Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder is: 3:3:4:3:3:4；
Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxygen
Changing in ferrous Thiobacillus mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is each about 6 × 109cfu/g；
Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is about 3%；
Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is about 4%；
Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is about 3%；
Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
After embodiment 2-5 all uses method same as in Example 1 to process, record the degradation rate knot of oil in pedotheque
Fruit is as follows:
Claims (7)
- The most petroleum-polluted soil-repairing agent, including the component of following portions by weight:Chitosan 1-3 bentonite 80-200Chlorella 10-25 citric acid 0.5-2Conch meal 2-9 Flammulina velutiper (Fr.) Sing mushroom bran 5-15Corn cob 40-80 Testa oryzae 10-20Tea seed episperm 5-15 walnut shell 5-20Betelnut tree wood flour 20-80 palm wood flour 25-60Compound enzymic preparation 0.001-0.02；Described compound enzymic preparation includes aldehyde dehydrogenase, catalase, nitroreductase；The enzyme of described aldehyde dehydrogenase alive 5.4 × 105U/g；Catalatic enzyme alive 18.3 × 105U/g ；Nitroreductase Enzyme alive 8.9 × 105U/g；Aldehyde dehydrogenase, catalase, the ratio of weight and number of nitroreductase are 1-4:2-6:1-5；Complex micro organism fungicide 0.005-0.048；The active component of described complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk Yeast mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；Described lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, Thiobacillus ferrooxidant mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder be: (1-4): (1-5): (2-5): (1-6): (1-5): (2- 5)；Described lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, In thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is 1 × 108-9×109cfu/g。
- The most petroleum-polluted soil-repairing agent, it is characterised in that the weight ratio of each mycopowder is:Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxidation Asia Ferrum Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder be: 3:3:4:3:3:4.
- The most petroleum-polluted soil-repairing agent, it is characterised in that described compound enzymic preparation is 0.008 part.
- The most petroleum-polluted soil-repairing agent, it is characterised in that described complex micro organism fungicide 0.036 part.
- The most petroleum-polluted soil-repairing agent, it is characterised in that include the group of following portions by weight Point:Chitosan 2 bentonite 160Chlorella 20 citric acid 1.5Conch meal 6 Flammulina velutiper (Fr.) Sing mushroom bran 10Corn cob 60 Testa oryzae 15Tea seed episperm 10 walnut shell 15Betelnut tree wood flour 60 palm wood flour 45Compound enzymic preparation 0.008 complex micro organism fungicide 0.036；Described compound enzymic preparation includes aldehyde dehydrogenase, catalase, nitroreductase；The enzyme of described aldehyde dehydrogenase alive 5.4 × 105U/g；Catalase 18.3 × 105U/g ；Nitroreductase 8.9 × 105U/g；Aldehyde dehydrogenase, catalase, the ratio of weight and number of nitroreductase are 2:4:3；The active component of described complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, white false silk Yeast mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；Described lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, Thiobacillus ferrooxidant mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder be: 3:3:4:3:3:4；Described lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, In thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is 1 × 108-9×109cfu/g。
- The most petroleum-polluted soil-repairing agent, it is characterised in that include the group of following portions by weight Point:Chitosan 2 bentonite 160Chlorella 20 citric acid 1.5Conch meal 6 Flammulina velutiper (Fr.) Sing mushroom bran 10Corn cob 60 Testa oryzae 15Tea seed episperm 10 walnut shell 15Betelnut tree wood flour 60 palm wood flour 45；Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is 2-5%；Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 2-6%；Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is 1-5%；Betelnut tree wood flour, palm wood flour be dry be 2-5% to moisture after and the bits shape thing pulverized；Complex micro organism fungicide 0.036 part；The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium Mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxidation Asia Ferrum Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder be: 3:3:4:3:3:4；Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxidation Asia In ferrum Thiobacillus mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is 2 × 109cfu/g；Compound enzymic preparation 0.008 part, compound enzymic preparation is by aldehyde dehydrogenase, catalase, nitroreductase, thioesterase institute group Become；The enzyme work of aldehyde dehydrogenase is 5.4 × 105U/g；The work of catalatic enzyme is 18.3 × 105U/g ；The enzyme of nitroreductase Work is 8.9 × 105U/g, the enzyme work of thioesterase are 11.3 × 105U/g；Aldehyde dehydrogenase, catalase, nitroreductase, the ratio of weight and number of thioesterase are 2:4:3:2.
- The most petroleum-polluted soil-repairing agent, it is characterised in that include the group of following portions by weight Point:Chitosan 2.5 bentonite 160Chlorella 18 citric acid 1.5Conch meal 7 Flammulina velutiper (Fr.) Sing mushroom bran 12Corn cob 60 Testa oryzae 16Tea seed episperm 12 walnut shell 18Betelnut tree wood flour 60 palm wood flour 50Compound enzymic preparation 0.012；Compound enzymic preparation is made up of aldehyde dehydrogenase, catalase, nitroreductase, Enone reductase；The enzyme of aldehyde dehydrogenase alive 5.4 × 105U/g；Catalatic enzyme alive 18.3 × 105U/g ；The enzyme of nitroreductase is lived 8.9×105U/g, the enzyme work of Enone reductase are 11.3 × 105U/g；Aldehyde dehydrogenase, catalase, nitroreductase, the ratio of weight and number of Enone reductase are 2:5:2:4；Complex micro organism fungicide 0.036 part；The active component of complex micro organism fungicide is by locking shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium Mycopowder, Rhodococcus marinonascens mycopowder, thiobacillus ferrooxidant mycopowder, Rhodococcus chlorophenolicus mycopowder are formed；Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxidation Asia Ferrum Thiobacillus mycopowder, the weight ratio of Rhodococcus chlorophenolicus mycopowder be: 3:3:4:3:3:4；Lock shadow yeast bacterium mycopowder, bacillus megaterium mycopowder, candida albicans bacterium mycopowder, Rhodococcus marinonascens mycopowder, oxidation Asia In ferrum Thiobacillus mycopowder, Rhodococcus chlorophenolicus mycopowder, living bacteria count is 6 × 109cfu/g；Chlorella is to shine the algae powder after dry doubling is pulverized, and its water content is 3%；Corn cob is to shine the particulate matter after dry doubling is pulverized, and its water content is 4%；Walnut shell is also for shining the powder after dry doubling is pulverized, and its water content is 3%；Betelnut tree wood flour, palm wood flour be dry be 4% to moisture after and the bits shape thing pulverized.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610438118.1A CN106085448A (en) | 2016-06-17 | 2016-06-17 | Oil-polluted soils renovation agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610438118.1A CN106085448A (en) | 2016-06-17 | 2016-06-17 | Oil-polluted soils renovation agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106085448A true CN106085448A (en) | 2016-11-09 |
Family
ID=57236506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610438118.1A Pending CN106085448A (en) | 2016-06-17 | 2016-06-17 | Oil-polluted soils renovation agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106085448A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635033A (en) * | 2016-12-04 | 2017-05-10 | 钦州市星火计划办公室 | Soil remediation agent and preparation method thereof |
| CN107254322A (en) * | 2017-08-18 | 2017-10-17 | 中山市绿浪助剂有限公司 | A kind of soil-repairing agent containing microbial bacterial agent |
| CN108823195A (en) * | 2018-06-15 | 2018-11-16 | 中国石油大学(华东) | A kind of preparation method and application method of mushroom bran immobilized microbial inoculum |
| CN109438088A (en) * | 2018-12-27 | 2019-03-08 | 天津天丰泽田生物科技有限公司 | A kind of microbe soil conditioner and preparation method thereof for remedying oil-polluted soils |
| CN110527515A (en) * | 2019-08-20 | 2019-12-03 | 北京上水环境科技发展有限公司 | A kind of petroleum hydrocarbon contaminated soil renovation agent and its preparation and application method |
| CN110724534A (en) * | 2019-10-11 | 2020-01-24 | 云南探花农业科技开发有限公司 | Powdery biological soil conditioner |
| CN112592722A (en) * | 2020-12-11 | 2021-04-02 | 贵州大学 | Modified biochar soil remediation agent as well as preparation method and application thereof |
| CN112974513A (en) * | 2021-02-22 | 2021-06-18 | 北部湾大学 | Method for bioremediation of petroleum-contaminated soil |
| CN114075511A (en) * | 2021-11-22 | 2022-02-22 | 山东科环环境管理咨询有限公司 | Petroleum-polluted soil remediation agent and preparation process thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484447A (en) * | 2013-09-29 | 2014-01-01 | 山东省科学院生物研究所 | Preparation method and application of petroleum degrading enzyme preparation |
| CN104694427A (en) * | 2015-02-12 | 2015-06-10 | 李丽萍 | Preparation process for microbial preparation for repairing nitrotoluene contaminated soil |
| CN104694428A (en) * | 2015-02-12 | 2015-06-10 | 何颖霞 | Microbial preparation for removing nitrotoluene in soil |
| CN104741374A (en) * | 2013-12-27 | 2015-07-01 | 中国科学院沈阳应用生态研究所 | Biology restoration method of petroleum pollution soil by using broad-host-range self-transfer degrading plasmid |
| CN104762210A (en) * | 2014-12-23 | 2015-07-08 | 中国科学院沈阳应用生态研究所 | Alkane degrading enzyme preparation, and preparation method and application thereof |
-
2016
- 2016-06-17 CN CN201610438118.1A patent/CN106085448A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484447A (en) * | 2013-09-29 | 2014-01-01 | 山东省科学院生物研究所 | Preparation method and application of petroleum degrading enzyme preparation |
| CN104741374A (en) * | 2013-12-27 | 2015-07-01 | 中国科学院沈阳应用生态研究所 | Biology restoration method of petroleum pollution soil by using broad-host-range self-transfer degrading plasmid |
| CN104762210A (en) * | 2014-12-23 | 2015-07-08 | 中国科学院沈阳应用生态研究所 | Alkane degrading enzyme preparation, and preparation method and application thereof |
| CN104694427A (en) * | 2015-02-12 | 2015-06-10 | 李丽萍 | Preparation process for microbial preparation for repairing nitrotoluene contaminated soil |
| CN104694428A (en) * | 2015-02-12 | 2015-06-10 | 何颖霞 | Microbial preparation for removing nitrotoluene in soil |
Non-Patent Citations (2)
| Title |
|---|
| 张甲耀等编著: "《环境微生物学》", 31 December 2018 * |
| 王友保编著: "《土壤污染与生态修复实验指导》", 30 June 2015 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635033A (en) * | 2016-12-04 | 2017-05-10 | 钦州市星火计划办公室 | Soil remediation agent and preparation method thereof |
| CN107254322A (en) * | 2017-08-18 | 2017-10-17 | 中山市绿浪助剂有限公司 | A kind of soil-repairing agent containing microbial bacterial agent |
| CN108823195A (en) * | 2018-06-15 | 2018-11-16 | 中国石油大学(华东) | A kind of preparation method and application method of mushroom bran immobilized microbial inoculum |
| CN109438088A (en) * | 2018-12-27 | 2019-03-08 | 天津天丰泽田生物科技有限公司 | A kind of microbe soil conditioner and preparation method thereof for remedying oil-polluted soils |
| CN110527515A (en) * | 2019-08-20 | 2019-12-03 | 北京上水环境科技发展有限公司 | A kind of petroleum hydrocarbon contaminated soil renovation agent and its preparation and application method |
| CN110724534A (en) * | 2019-10-11 | 2020-01-24 | 云南探花农业科技开发有限公司 | Powdery biological soil conditioner |
| CN112592722A (en) * | 2020-12-11 | 2021-04-02 | 贵州大学 | Modified biochar soil remediation agent as well as preparation method and application thereof |
| CN112974513A (en) * | 2021-02-22 | 2021-06-18 | 北部湾大学 | Method for bioremediation of petroleum-contaminated soil |
| CN114075511A (en) * | 2021-11-22 | 2022-02-22 | 山东科环环境管理咨询有限公司 | Petroleum-polluted soil remediation agent and preparation process thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106085448A (en) | Oil-polluted soils renovation agent | |
| CN106047363A (en) | Petroleum-polluted soil repairing material | |
| Gan et al. | Remediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs) | |
| Koolivand et al. | Oily sludge biodegradation using a new two-phase composting method: kinetics studies and effect of aeration rate and mode | |
| CN107376940A (en) | A kind of catalytic composite material of rapidly and efficiently renovation of organic pollution soil and preparation method thereof | |
| CN108637005A (en) | It is a kind of to repair polycyclic aromatic hydrocarbons contaminated composite material, preparation method and the usage | |
| Mulbry et al. | Biodegradation of the organophosphate insecticide coumaphos in highly contaminated soils and in liquid wastes | |
| CN108486006B (en) | A kind of preparation method and application of greasy filth oil degradation complex enzyme | |
| CN106010565A (en) | Organochlorine pesticide contaminated soil remediation agent | |
| CN106734188A (en) | A kind of nitrate nitrogen contamination method of heavy metals in farmland pollution | |
| CN106085450A (en) | Heavy-metal contaminated soil repair materials | |
| CN104838996A (en) | Method of using chlorpyrifos and CNT (carbon nanometer tube) interlayer to regulate lawn grass heavy metal accumulation | |
| CN108315015A (en) | Compounding renovation agent and its restorative procedure for clearing up petroleum-polluted soil | |
| CN109370596A (en) | A kind of in-situ passivation the reparation medicament, preparation method and application of cadmium arsenic combined pollution agricultural land soil | |
| CN108188162A (en) | A kind of serious pollution land method | |
| CN107365227A (en) | Heavy-metal contaminated soil compost modifying agent and its modification method | |
| CN106118670A (en) | Organochlorine pesticide pollution soil remediation material | |
| CN105478459A (en) | Method for treating petroleum-lead-cadmium combined polluted soil | |
| Liu et al. | Remediation of weathered diesel-oil contaminated soils using biopile systems: An amendment selection and pilot-scale study | |
| CN109455891A (en) | A kind of compound bio preparation for repairing for oily sludge | |
| CN101654661B (en) | Bioremediation method for petroleum contamination and its special strains | |
| CN101654662B (en) | Method for decomposing petroleum hydrocarbon and special bacterial strain thereof | |
| CN106047365A (en) | Mercury-contaminated soil remediation agent | |
| Strizhenok et al. | Assessment of the efficiency of using organic waste from the brewing industry for bioremediation of oil-contaminated soils | |
| RU2421291C2 (en) | Method to reclaim soils contaminated with oil and oil products |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161109 |
|
| RJ01 | Rejection of invention patent application after publication |