CN106075551A - A kind of preparation method of intercrossed network type Pseudobulbus Bletillae polysaccharose film - Google Patents
A kind of preparation method of intercrossed network type Pseudobulbus Bletillae polysaccharose film Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0042—Materials resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
The present invention relates to the preparation method of a kind of intercrossed network type Pseudobulbus Bletillae polysaccharose film, belong to Textile Engineering field.The preparation method of the present invention is by soluble in water for the Pseudobulbus Bletillae polysaccharose powder extracted from natural Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, obtain Pseudobulbus Bletillae polysaccharose solution, utilizing two-way ice crystal induced growth techinque to order about Pseudobulbus Bletillae polysaccharose solution and be fully cured molding, the lyophilization under vacuum low-temperature environment of the Pseudobulbus Bletillae polysaccharose solution after molding is formed has intercrossed network type polysaccharide membrane material.The preparation method technique of the present invention is simple, with low cost, without any chemical reagent, without any harmful substance, synusia within intercrossed network type Pseudobulbus Bletillae polysaccharose film prepared by this method is the mutual arrangement architecture of rule, there is good mechanical property, be suitable for application in functional auxiliary materials and field of tissue engineering technology.
Description
Technical field
The present invention relates to the preparation method of a kind of intercrossed network type Pseudobulbus Bletillae polysaccharose film, belong to Textile Engineering technical field.
Background technology
Pseudobulbus Bletillae polysaccharose has an effect such as anti-inflammation, putrefaction-removing granulation-promoting, the convergence open sore part, and have no side effect, safety and comfort,
Degradable, pollution-free, biocompatibility, it is a kind of good natural biological engineering material, medicine can be applied to as excipient substance
Thing preparation.
In bio-medical material, Pseudobulbus Bletillae polysaccharose is widely used.China Patent Publication No. is
CN102961776A, publication date is on 03 13rd, 2013, invention entitled: the preparation method of a kind of bletilla striata hemostatic gauze,
This product is made into the Pseudobulbus Bletillae polysaccharose solution of certain solid content by extracting Bletilla glucomannan, is spun into long filament with dehydrated alcohol for coagulating bath,
Using knitting, woven or non-woven fabrics method is made.Water-soluble hemostatic gauze prepared by the method has good antibacterial, antiinflammatory,
The curative effect of quick-acting haemostatic powder, significantly improves the speed of wound healing, has a good application prospect at biomedicine field.But by
Relatively big in Pseudobulbus Bletillae polysaccharose fiber stiffness, the bletilla striata hemostatic gauze use that it is prepared easily stimulates wound, simultaneously in bleeding tissue
Position does not has good adhesion polarization and pull-out capacity, reduces haemostatic effect, easily extends the recovery time of wound.Chinese patent
Publication number is CN102477102A, and publication date is on 05 30th, 2012, invention entitled: a kind of Pseudobulbus Bletillae polysaccharose is used for stopping blooding
The method of material, the Pseudobulbus Bletillae polysaccharose coarse powder that this invention will be extracted from Pseudobulbus Bletillae (Rhizoma Bletillae) tuber, make Pseudobulbus Bletillae polysaccharose aqueous solution, at low temperature and
Make spongy material after processing under vacuum environment, strengthen the adhesion polarization at bleeding tissue position and pull-out capacity, further
Expand the application of Pseudobulbus Bletillae polysaccharose.But sponge material internal structure design is fine not, absorb penetrating fluid ability poor.In
State's patent publication No. is CN104927071A, and publication date is on 09 23rd, 2015, invention entitled: a kind of highly oriented polysaccharide
The preparation method of fibrous membrane, this invention utilizes orientation temperature field freezing technology to control ice during macromolecular polysaccharide freezing solution
Crystals growth direction, the polysaccharide solution after freezing forms the polysaccharide fiber with highly oriented folded page structure under vacuum low-temperature environment
Film, the arrangement of fibrous membrane internal page sheet rule, interlayer connectedness is good, and attachment and growth for cell improve favourable environment.
But being that the degree that intersects between simple parallel folded page structure is low inside fibrous membrane, in use, blood easily breaks through
Fibrous membrane, directly reduces the haemostatic effect of fibrous membrane.
Summary of the invention
For above-mentioned existing problems, it is an object of the invention to provide the preparation side of a kind of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Method, for achieving the above object, technical scheme:
The preparation method of a kind of intercrossed network type Pseudobulbus Bletillae polysaccharose film, including the extraction of Pseudobulbus Bletillae polysaccharose and Pseudobulbus Bletillae polysaccharose solution
Preparation, described preparation method refers to:
The Pseudobulbus Bletillae polysaccharose solution prepared is poured in the groove of flat plate mold, an adjacent face of flat plate mold divides
Be not provided with transmission temperature metal transducer, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in vacuum be 0.05～
In the environment of 0.1MPa, 2～5h remove the bubble in Pseudobulbus Bletillae polysaccharose solution, then wrap up flat plate mold with heat-barrier material, by flat board
Die horizontal be positioned over-50～-80 DEG C of environment in, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, formed 3～
The cured layer of 8mm thickness, then the cured layer after the demoulding is placed in temperature be-60～-80 DEG C, vacuum be 2～5Pa environment in
Lyophilization, takes out after 24～36h, i.e. obtains intercrossed network type Pseudobulbus Bletillae polysaccharose film.
The material of described flat plate mold is in politef or polypropylene or polyethylene or polymethyl methacrylate
One.
Described heat-barrier material is the one in polypropylene screen or polyethylene film or polyurethane foam.
Owing to using above technical scheme, the preparation method of the present invention utilizes two-way ice crystal induced growth techinque to make the Pseudobulbus Bletillae (Rhizoma Bletillae)
Polysaccharide solution fully charge molding, by the metal transducer being arranged on flat plate mold is lowered the temperature, metal transducer
Corresponding one end, one end forms a temperature difference, makes the moisture in solution freeze, orders about the Pseudobulbus Bletillae (Rhizoma Bletillae) in Pseudobulbus Bletillae polysaccharose solution many
Glycan molecule separates out from solution, forms laminated structure by the squeezing action power between ice and ice simultaneously.Due to simultaneously at two
Ice crystal driving force is applied on direction, and orthogonal between driving force, the network of each self-forming independence, simultaneously network between mutually
Intersect, define inierpeneirating network structure, between horizontal and vertical, set up corresponding junction point, can be that Pseudobulbus Bletillae polysaccharose film provides
Enough mechanical strengths.
Pseudobulbus Bletillae polysaccharose solution is in freezing solidification process, and present in solution, bubble can destroy the IPN net of Pseudobulbus Bletillae polysaccharose film
Network structure, must be placed in the environment that vacuum is 0.05～0.1MPa by the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution before therefore freezing
In 2～5h remove bubble removing.
Form a bigger temperature difference in order to ensure corresponding one end, metal transducer one end in freezing process, make
Pseudobulbus Bletillae polysaccharose solution at short notice can fully charge, can get the high intensity Pseudobulbus Bletillae (Rhizoma Bletillae) base dressing of structural integrity simultaneously.Flat
The material of board mold mainly selects heat conductivility poor and the politef of easy acquisition or polypropylene or polyethylene or poly-first
Base acrylic acid methyl ester., wraps up flat plate mold with heat-barrier material before freezing simultaneously, and lapping mainly selects heat conductivility relatively
Difference and the polypropylene screen easily obtained and polyethylene film material and polyurethane foam.
The thickness of film determines the water absorption of film, breathability, flexibility, and cured layer thickness is the least, not easy-formation, after film forming
Material easily ruptures, and the thickness of cured layer is too big, and permeability after film forming is the poorest has the highest modulus simultaneously, unfavorable
In the application in later stage, by controlling the thickness of cured layer in the 3～8mm thickness that can effectively control intercrossed network type Pseudobulbus Bletillae polysaccharose film
Degree.
The raw material that the preparation method of the present invention is used is to extract, from Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, the Pseudobulbus Bletillae polysaccharose obtained, raw for one
The thing compatibility is good and can be dissolved in the material of water very well, and therefore prepared Pseudobulbus Bletillae polysaccharose film can be fully absorbed by human body.Utilize
Two-way ice crystal induced growth forms the most cross one another network structure of one, and having constructed binding site at network is Pseudobulbus Bletillae polysaccharose film
Provide good mechanical property, on the basis of giving full play to Pseudobulbus Bletillae (Rhizoma Bletillae) macromolecular material haemostatic effect, improve the mechanics of adjuvant
Performance, expands its corresponding application.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail.
A kind of intercrossed network type Pseudobulbus Bletillae polysaccharose membrane preparation method, the wherein extraction of Pseudobulbus Bletillae polysaccharose and the system of Pseudobulbus Bletillae polysaccharose solution
Being CN102961776A for see China Patent Publication No., publication date is on 03 13rd, 2013, invention entitled: a kind of
The preparation method of bletilla striata hemostatic gauze.Described preparation method sequentially includes the following steps:
A. the extraction of Pseudobulbus Bletillae polysaccharose
Dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block and distilled water are soaked with the ratio of mass ratio 1:5～1:10, soak time be 0.5～
2h, soaking temperature is 40～80 DEG C, filtered through gauze, collects Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 4～10 times, merge and receive by several times
Collection Pseudobulbus Bletillae polysaccharose filtrate, be placed in the air dry oven of 50～100 DEG C concentrate, it is thus achieved that mass concentration be 1.5～5% white
Splendid achnatherum polysaccharide concentrated solution.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol be Pseudobulbus Bletillae polysaccharose concentrated solution volume 1～
2 times, washing precipitation number of times is 2～5 times, and each wash time is 1～5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in temperature is 40
～the baking oven of 80 DEG C is dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 60～70 DEG C, use 300～500
Turn/the mixing speed of min stirring mixing 3～5h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 2～5%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared pours politef or polypropylene or polyethylene or polymethylacrylic acid into
In the groove of methyl ester flat plate mold, an adjacent face of flat plate mold is respectively arranged with the metal transducer of transmission temperature,
The flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in 2～5h removal Pseudobulbus Bletillae polysaccharose in the environment that vacuum is 0.05～0.1MPa
Bubble in solution, then wraps up flat plate mold, by flat plate mold level by polypropylene screen or polyethylene film or polyurethane foam
Be positioned over-50～-80 DEG C of environment in, temperature is conducted by the metal transducer with transmission temperature by inside export-oriented solution,
Corresponding one end, one end of metal transducer forms a temperature difference, makes the moisture in solution freeze, orders about Pseudobulbus Bletillae polysaccharose molten
Pseudobulbus Bletillae polysaccharose molecule in liquid separates out from solution, forms laminated structure by the squeezing action power between ice and ice simultaneously.By
Ice crystal driving force is applied in upper simultaneously in two directions, and orthogonal between driving force, the network of each self-forming independence, simultaneously
Cross one another between network, define inierpeneirating network structure, form the cured layer of 3～8mm thickness, then by the cured layer after the demoulding
Be placed in temperature be-60～-80 DEG C, vacuum be 2～5Pa environment in lyophilization, after 24～36h take out, i.e. obtain IPN
Network-type Pseudobulbus Bletillae polysaccharose film.
Specific embodiment
It is prepared as described above method.
Embodiment one
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:5 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 0.5h, soaking temperature
It is 40 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 4 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 50 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 1.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 1 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 2 times, and each wash time is 1min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in the baking that temperature is 40 DEG C
Case is dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 60 DEG C, use stirring of 300 turns/min
Mix speed stirring mixing 3h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 2%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polytetrafluoroethylene mould, the one of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on individual adjacent face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in very
Reciprocal of duty cycle be 0.05MPa environment in 2h remove the bubble in Pseudobulbus Bletillae polysaccharose solution, then wrap up flat plate mold with polypropylene screen, will
Flat plate mold is placed horizontally in-50 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, forms 3mm
The cured layer of thickness, then the cured layer after the demoulding is placed in temperature be-60 DEG C, vacuum be 5Pa environment in lyophilization,
Take out after 36h, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment two
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:6 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 1h, and soaking temperature is
55 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 6 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 65 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 2.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 1 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 3 times, and each wash time is 1～5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in temperature is 40～80
DEG C baking oven in be dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 63 DEG C, use stirring of 370 turns/min
Mix speed stirring mixing 3h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 3%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of Polypropylene plates mould, a phase of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on neighboring terminal face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in vacuum
Remove the bubble in Pseudobulbus Bletillae polysaccharose solution for 3h in the environment of 0.07MPa, then wrap up flat plate mold with polypropylene screen, by flat board
Die horizontal is positioned in-60 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, forms 5mm thickness
Cured layer, then the cured layer after the demoulding is placed in temperature be-65 DEG C, vacuum be 4Pa environment in lyophilization, after 32h
Take out, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment three
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:8 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 1.5h, soaking temperature
It is 60 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 8 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 85 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 3.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 2 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 4 times, and each wash time is 1～5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in temperature is 40～80
DEG C baking oven in be dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 67 DEG C, use stirring of 430 turns/min
Mix speed stirring mixing 4h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 4%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polyethylene flat plate mold, a phase of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on neighboring terminal face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in vacuum
Remove the bubble in Pseudobulbus Bletillae polysaccharose solution for 4h in the environment of 0.09MPa, then wrap up flat plate mold with polypropylene screen, by flat board
Die horizontal is positioned in-70 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, forms 6mm thickness
Cured layer, then the cured layer after the demoulding is placed in temperature be-75 DEG C, vacuum be 3Pa environment in lyophilization, after 28h
Take out, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment four
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:10 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 2h, soaking temperature
It is 80 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 10 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 100 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentrated solution of 5%.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 2 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 5 times, and each wash time is 5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in the baking that temperature is 80 DEG C
Case is dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 70 DEG C, use stirring of 500 turns/min
Mix speed stirring mixing 5h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 5%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polymethyl methacrylate flat plate mold, flat board mould
It is respectively arranged with the metal transducer of transmission temperature on one adjacent face of tool, the flat plate mold of Pseudobulbus Bletillae polysaccharose solution will be filled
It is placed in the bubble during 5h removes Pseudobulbus Bletillae polysaccharose solution in the environment that vacuum is 0.1MPa, then wraps up flat board mould with polypropylene screen
Tool, is placed horizontally at flat plate mold in-80 DEG C of environment, until the fully charge molding of Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution, shape in flat plate mold
Become the cured layer of 8mm thickness, then the cured layer after the demoulding is placed in temperature be-80 DEG C, vacuum be 2Pa environment in freezing dry
Dry, take out after 24h, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment five
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:5 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 0.5h, soaking temperature
It is 40 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 4 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 50 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 1.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 1 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 2 times, and each wash time is 1min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in the baking that temperature is 40 DEG C
Case is dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 60 DEG C, use stirring of 300 turns/min
Mix speed stirring mixing 3h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 2%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polytetrafluoroethylene mould, the one of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on individual adjacent face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in very
Reciprocal of duty cycle be 0.05MPa environment in 2h remove the bubble in Pseudobulbus Bletillae polysaccharose solution, then wrap up flat plate mold with polyethylene film, will
Flat plate mold is placed horizontally in-50 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, forms 3mm
The cured layer of thickness, then the cured layer after the demoulding is placed in temperature be-60 DEG C, vacuum be 5Pa environment in lyophilization,
Take out after 36h, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment six
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:6 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 1h, and soaking temperature is
55 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 6 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 65 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 2.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 1 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 3 times, and each wash time is 1～5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in temperature is 40～80
DEG C baking oven in be dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 63 DEG C, use stirring of 370 turns/min
Mix speed stirring mixing 3h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 3%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of Polypropylene plates mould, a phase of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on neighboring terminal face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in vacuum
Remove the bubble in Pseudobulbus Bletillae polysaccharose solution for 3h in the environment of 0.07MPa, then wrap up flat plate mold with polyethylene film, by flat board
Die horizontal is positioned in-60 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, forms 5mm thickness
Cured layer, then the cured layer after the demoulding is placed in temperature be-65 DEG C, vacuum be 4Pa environment in lyophilization, after 32h
Take out, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment seven
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:8 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 1.5h, soaking temperature
It is 60 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 8 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 85 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 3.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 2 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 4 times, and each wash time is 1～5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in temperature is 40～80
DEG C baking oven in be dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 67 DEG C, use stirring of 430 turns/min
Mix speed stirring mixing 4h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 4%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polyethylene flat plate mold, a phase of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on neighboring terminal face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in vacuum
Remove the bubble in Pseudobulbus Bletillae polysaccharose solution for 4h in the environment of 0.09MPa, then wrap up flat plate mold with polyethylene film, by flat board
Die horizontal is positioned in-70 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, forms 6mm thickness
Cured layer, then the cured layer after the demoulding is placed in temperature be-75 DEG C, vacuum be 3Pa environment in lyophilization, after 28h
Take out, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment eight
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:10 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 2h, soaking temperature
It is 80 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 10 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 100 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentrated solution of 5%.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 2 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 5 times, and each wash time is 5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in the baking that temperature is 80 DEG C
Case is dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 70 DEG C, use stirring of 500 turns/min
Mix speed stirring mixing 5h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 5%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polymethyl methacrylate flat plate mold, flat board mould
It is respectively arranged with the metal transducer of transmission temperature on one adjacent face of tool, the flat plate mold of Pseudobulbus Bletillae polysaccharose solution will be filled
It is placed in the bubble during 5h removes Pseudobulbus Bletillae polysaccharose solution in the environment that vacuum is 0.1MPa, then wraps up flat board mould with polyethylene film
Tool, is placed horizontally at flat plate mold in-80 DEG C of environment, until the fully charge molding of Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution, shape in flat plate mold
Become the cured layer of 8mm thickness, then the cured layer after the demoulding is placed in temperature be-80 DEG C, vacuum be 2Pa environment in freezing dry
Dry, take out after 24h, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment nine
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:5 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 0.5h, soaking temperature
It is 40 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 4 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 50 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 1.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 1 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 2 times, and each wash time is 1min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in the baking that temperature is 40 DEG C
Case is dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 60 DEG C, use stirring of 300 turns/min
Mix speed stirring mixing 3h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 2%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polytetrafluoroethylene mould, the one of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on individual adjacent face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in very
Reciprocal of duty cycle be 0.05MPa environment in 2h remove the bubble in Pseudobulbus Bletillae polysaccharose solution, then wrap up flat plate mold by polyurethane foam,
Flat plate mold is placed horizontally in-50 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, is formed
The cured layer of 3mm thickness, then the cured layer after the demoulding is placed in temperature be-60 DEG C, vacuum be 5Pa environment in freezing dry
Dry, take out after 36h, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment ten
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:6 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 1h, and soaking temperature is
55 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 6 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 65 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 2.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 1 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 3 times, and each wash time is 1～5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in temperature is 40～80
DEG C baking oven in be dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 63 DEG C, use stirring of 370 turns/min
Mix speed stirring mixing 3h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 3%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of Polypropylene plates mould, a phase of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on neighboring terminal face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in vacuum
Remove the bubble in Pseudobulbus Bletillae polysaccharose solution for 3h in the environment of 0.07MPa, then wrap up flat plate mold by polyurethane foam, will be flat
Board mold is placed horizontally in-60 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, forms 5mm thick
The cured layer of degree, then the cured layer after the demoulding is placed in temperature be-65 DEG C, vacuum be 4Pa environment in lyophilization, 32h
Rear taking-up, i.e. obtains intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment 11
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:8 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 1.5h, soaking temperature
It is 60 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 8 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 85 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentration of 3.5%
Liquid.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 2 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 4 times, and each wash time is 1～5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in temperature is 40～80
DEG C baking oven in be dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 67 DEG C, use stirring of 430 turns/min
Mix speed stirring mixing 4h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 4%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polyethylene flat plate mold, a phase of flat plate mold
It is respectively arranged with the metal transducer of transmission temperature on neighboring terminal face, the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in vacuum
Remove the bubble in Pseudobulbus Bletillae polysaccharose solution for 4h in the environment of 0.09MPa, then wrap up flat plate mold by polyurethane foam, will be flat
Board mold is placed horizontally in-70 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, forms 6mm thick
The cured layer of degree, then the cured layer after the demoulding is placed in temperature be-75 DEG C, vacuum be 3Pa environment in lyophilization, 28h
Rear taking-up, i.e. obtains intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Embodiment 12
A. the extraction of Pseudobulbus Bletillae polysaccharose
Being soaked with the ratio of mass ratio 1:10 with distilled water by dry Pseudobulbus Bletillae (Rhizoma Bletillae) stem block, soak time is 2h, soaking temperature
It is 80 DEG C, filtered through gauze, collect Pseudobulbus Bletillae polysaccharose filtrate.
Extract the Pseudobulbus Bletillae (Rhizoma Bletillae) stem block after Pseudobulbus Bletillae polysaccharose filtrate and continue above immersion and filter process 10 times, merge and collect by several times
Pseudobulbus Bletillae polysaccharose filtrate, is placed in the air dry oven of 100 DEG C concentrating, it is thus achieved that mass concentration is the Pseudobulbus Bletillae polysaccharose concentrated solution of 5%.
In the Pseudobulbus Bletillae polysaccharose concentrated solution of above-mentioned acquisition, add absolute ethyl alcohol and stirring, precipitate with ethanol, be filtrated to get precipitation, and use nothing
The precipitation that water-ethanol washing and filtering obtains, wherein, the volume of dehydrated alcohol added by precipitate with ethanol is the 2 of Pseudobulbus Bletillae polysaccharose concentrated solution volume
Times, washing precipitation number of times is 5 times, and each wash time is 5min, and the Pseudobulbus Bletillae polysaccharose after washing is placed in the baking that temperature is 80 DEG C
Case is dried, dried Pseudobulbus Bletillae polysaccharose is pulverized, i.e. obtains Pseudobulbus Bletillae polysaccharose powder.
B. the preparation of Pseudobulbus Bletillae polysaccharose solution
The Pseudobulbus Bletillae polysaccharose powder of said extracted is joined in the distilled water that temperature is 70 DEG C, use stirring of 500 turns/min
Mix speed stirring mixing 5h, make the Pseudobulbus Bletillae polysaccharose solution that mass fraction is 5%.
C. the preparation of intercrossed network type Pseudobulbus Bletillae polysaccharose film
Pseudobulbus Bletillae polysaccharose solution b step prepared is poured in the groove of polymethyl methacrylate flat plate mold, flat board mould
It is respectively arranged with the metal transducer of transmission temperature on one adjacent face of tool, the flat plate mold of Pseudobulbus Bletillae polysaccharose solution will be filled
It is placed in the bubble during 5h removes Pseudobulbus Bletillae polysaccharose solution in the environment that vacuum is 0.1MPa, then wraps up flat board by polyurethane foam
Mould, is placed horizontally at flat plate mold in-80 DEG C of environment, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold,
Form the cured layer of 8mm thickness, then the cured layer after the demoulding is placed in temperature be-80 DEG C, vacuum be 2Pa environment in freezing
It is dried, takes out after 24h, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
Claims (3)
1. a preparation method for intercrossed network type Pseudobulbus Bletillae polysaccharose film, including extraction and the system of Pseudobulbus Bletillae polysaccharose solution of Pseudobulbus Bletillae polysaccharose
Standby, it is characterised in that described preparation method refers to:
The Pseudobulbus Bletillae polysaccharose solution prepared is poured in the groove of flat plate mold, an adjacent face of flat plate mold sets respectively
Being equipped with the metal transducer of transmission temperature, it is 0.05～0.1MPa that the flat plate mold filling Pseudobulbus Bletillae polysaccharose solution is placed in vacuum
Environment in 2～5h remove the bubble in Pseudobulbus Bletillae polysaccharose solution, then wrap up flat plate mold with heat-barrier material, by flat plate mold water
Keep flat be placed in-50～-80 DEG C of environment in, until Pseudobulbus Bletillae (Rhizoma Bletillae) polysaccharide solution fully charge molding in flat plate mold, form 3～8mm thick
The cured layer of degree, then the cured layer after the demoulding is placed in temperature be-60～-80 DEG C, vacuum be 2～5Pa environment in freezing
It is dried, takes out after 24～36h, i.e. obtain intercrossed network type Pseudobulbus Bletillae polysaccharose film.
The preparation method of a kind of intercrossed network type Pseudobulbus Bletillae polysaccharose film the most as claimed in claim 1, it is characterised in that: described is flat
The material of board mold is the one in politef or polypropylene or polyethylene or polymethyl methacrylate.
The preparation method of a kind of intercrossed network type Pseudobulbus Bletillae polysaccharose film the most as claimed in claim 1, it is characterised in that: described every
Hot material is the one in polypropylene screen or polyethylene film or polyurethane foam.
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| CN110201210A (en) * | 2018-07-27 | 2019-09-06 | 武汉纺织大学 | A kind of preparation method of bletilla striata dressing easy to operate |
| CN110201209A (en) * | 2018-07-27 | 2019-09-06 | 武汉纺织大学 | A kind of preparation method of porous material enhancing bletilla striata dressing |
| CN110215535A (en) * | 2018-07-27 | 2019-09-10 | 武汉纺织大学 | A kind of preparation method of embedded structure formula bletilla striata dressing |
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