CN108904867A - A kind of preparation method of the embedded double-deck bletilla striata dressing - Google Patents
A kind of preparation method of the embedded double-deck bletilla striata dressing Download PDFInfo
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- CN108904867A CN108904867A CN201810851657.7A CN201810851657A CN108904867A CN 108904867 A CN108904867 A CN 108904867A CN 201810851657 A CN201810851657 A CN 201810851657A CN 108904867 A CN108904867 A CN 108904867A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/64—Use of materials characterised by their function or physical properties specially adapted to be resorbable inside the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
The present invention relates to a kind of preparation methods of embedded double-deck bletilla striata dressing, belong to biomedicine technical field.Preparation method, which is used from the bletilla polysaccharide that bletilla striata stem block extracts, is dissolved in water preparation casting solution, take part bletilla polysaccharide casting solution, with naoh treatment, obtain embeding layer casting solution, bletilla polysaccharide casting solution orientation is first freezed into shaping membrane, in type film surface cutting, then embeding layer casting solution is poured under liquid nitrogen frozen and is frozen into double deck type film on cutting type film, by double deck type film it is freeze-dried again after obtain the embedded double-deck bletilla striata dressing.The present invention prepares the double-deck bletilla striata dressing using cutting and freezing, in conjunction with reliable, simple process, overcomes bletilla striata dressing soluble adhesion gloves, the difficult problem for being detached from docile wound when taking.Gained bletilla striata dressing of the invention can be degraded and absorbed in vivo, good with biocompatible, without side-effects to wound, and orientation pore structure ensure that dressing to penetrating fluid efficient absorption, while keeping dressing soft, will not damage to wound.
Description
Technical field
The present invention relates to a kind of preparation methods of embedded double-deck bletilla striata dressing, belong to biomedicine technical field.
Background technique
In surgery clinical use, the quick-acting haemostatic powder of wound has important influence to the post-operative recovery of patient.The bletilla striata makees
For one of traditional Chinese medicine hemostatic material, tuber extract bletilla polysaccharide has splendid hemostatic and stronger antibacterial
Property, and be a kind of water-soluble substances.Bletilla polysaccharide is because it is with excellent astringing to arrest bleeding, antibacterial anti-inflammatory, the function removed necrosis and promoted granulation
Effect, and its it is biological it is good, biodegradable, have no toxic side effect, in art of wound dressings using increasingly extensive.Existing is white
Splendid achnatherum dressing is mainly made as porous structure, improves its water imbibition, then enhances its anthemorrhagic performance.It is wound after bletilla polysaccharide swelling
Wet environment is provided, accelerates the healing of wound, and the formation of scar can be inhibited.
China Patent Publication No. is CN106075551A, and publication date is on November 09th, 2016, entitled one kind
The preparation method of intercrossed network type bletilla polysaccharide film, the bletilla polysaccharide preparation solution soluble in water which obtains extraction, benefit
Freeze bletilla polysaccharide solution with two-way ice crystal induced growth techinque, obtaining using freeze-drying has intercrossed network type polysaccharide membrane
Material.The bletilla polysaccharide film of this method preparation can be fully absorbed by human body, and the binding site that network is constructed provides for bletilla polysaccharide film
Good mechanical property on the basis of giving full play to bletilla striata high molecular material haemostatic effect improves the mechanical property of dressing,
Expand its corresponding application field.China Patent Publication No. is CN106075536A, and publication date is on November 09th, 2016, hair
A kind of bright entitled preparation method of bletilla striata base dressing, the product construct fiber-reinforced layer by the way that bletilla polysaccharide is spun into fiber,
Fibre-reinforced bletilla striata base dressing is prepared using the oriented growth and Freeze Drying Technique of ice crystal.The bletilla striata base of this method preparation applies
Expect that internal synusia and long filament formation are mutually perpendicular to cross arrangement structure, good mechanical properties.The preparation method simple process, cost
It is cheap, do not add chemical reagent.But it exists when in use is not easy to take, and surface layer is soluble, is not easy docile wound, reduces it
Haemostatic effect and speed of wound healing.
Existing bletilla striata dressing is led in surgical operation using the reason of speckling with watery blood on the middle gloves that can be worn because of doctor
Dissolution and adhesion are caused, to be unfavorable for dressing in the docile of wound.
Summary of the invention
For above-mentioned there are problem, the purpose of the present invention is to provide a kind of embedded double-deck bletilla striata dressing preparation methods.
To achieve the above object, technical solution of the invention is：
A kind of preparation method of the embedded double-deck bletilla striata dressing, the preparation method carry out according to the following steps：
The extraction of a bletilla polysaccharide
By dry bletilla striata stem block and distilled water with mass ratio 1:3~1:10 ratio is impregnated, and soaking time is 3~
20min, soaking temperature are 25~40 DEG C, and filtered through gauze removes solvable and not soluble in water impurity, collect the preliminary clean bletilla striata
Stem block is in 30~50 DEG C of oven dryings 18~for 24 hours.
Bletilla striata stem block and dehydrated alcohol after preliminary cleaning is dried is with mass ratio 1:3~1:10 ratio is impregnated, and is impregnated
Time is 1~3h, and soaking temperature is 20~30 DEG C, and filtered through gauze removes soluble pigment, collects bletilla striata stem block in 30~50 DEG C
Oven drying 18~for 24 hours.
Bletilla striata stem block and distilled water after removing depigmentaton is with mass ratio 1:3~1:10 ratio is impregnated, and soaking time is
0.5~3h, soaking temperature are 50~90 DEG C, and filtered through gauze collects bletilla polysaccharide filtrate.
By extract bletilla polysaccharide filtrate after bletilla striata stem block continue extract bletilla polysaccharide filtrate immersion and filter process 3~
10 times, the mass conservation of added distilled water merges the bletilla polysaccharide filtrate collected by several times, is placed in 30~80 DEG C of rotary evaporation
It is concentrated in instrument, obtains the bletilla polysaccharide concentrate that mass concentration is 1.5~5%.
Absolute ethyl alcohol and stirring is added into bletilla polysaccharide concentrate, alcohol precipitation is obtained by filtration precipitating, and is washed with dehydrated alcohol
The precipitating being obtained by filtration, wherein the volume of dehydrated alcohol added by alcohol precipitation is 1~2 times of the bletilla polysaccharide volume of the concentrated liquid, and washing is heavy
Starch number is 2~10 times, and each wash time is 1~5min, and the bletilla polysaccharide after washing is placed in baking oven to dry, drying
Temperature is 40~80 DEG C, and dry bletilla polysaccharide is made.
The preparation of b bletilla polysaccharide casting solution
Drying bletilla polysaccharide prepared by step a is dissolved in water and makes the bletilla polysaccharide that mass fraction is 3~10%
Solution is placed in the vacuum state that vacuum degree is 0.1~0.5MPa and goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of c sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 1~4%, is stirred evenly cold
But to room temperature.
The preparation of d embeding layer casting solution
The bletilla polysaccharide casting solution prepared in 20~50% step b is taken, sodium hydroxide solution prepared by step c is with 0.3
The speed of~1.0ml/min is added drop-wise in the bletilla polysaccharide casting solution stirred under 200~500r/min speed, stirs 2~5h
Afterwards, it is placed in 3~6h in the environment that vacuum degree is 0.05~0.1MPa and removes bubble removing, obtain embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 96:4~99:1.
E bilayer bletilla striata dressing preparation
Bletilla polysaccharide casting solution prepared in step b is poured into the groove of flat plate mold, the bottom of perpendicular in groove
It is provided with the metal transducer of transmitting temperature on one side in face, flat plate mold is wrapped up with heat-barrier material, by flat plate mold water
It lays flat in the environment for being placed in -50~-80 DEG C, freezes shaping membrane until the bletilla polysaccharide casting solution in flat plate mold is sufficiently directional,
Formed 3~8mm thickness bletilla polysaccharide film, type film is taken out, with sharp inverted T shaped slotting cutter type film upper surface with 1~2/
The density of cm scribes inverted T shaped groove, and the lower width of rebate of inverted T shaped groove is 1.5~3mm, and the width ratio of lower notch and upper notch is
2:1~3:1, the depth of inverted T shaped groove is 1~2mm, and upper notch and lower depth of rebate ratio are 1:1~1:2, upper notch is located at type
Type film cutting is put into the flat plate mold groove of above-mentioned not set metal transducer upwardly, is prepared by step d by film upper surface
Embeding layer casting solution pour into the upper layer for having carved the type film of slot, embeding layer casting solution is with volume ratio used in bletilla polysaccharide casting solution
0.2:1~1:1, layer casting solution to be embedded is full of cutting, entire mold is placed in heat-insulated container, liquid is poured into container
Nitrogen, liquid nitrogen upper surface are no more than cutting bottom surface and remove mold, the double deck type that will freeze after all solution are fully cured in mold
Film is placed in that temperature is -60~-80 DEG C, vacuum degree is to be freeze-dried in the environment of 1~5Pa, takes out, that is, obtains embedding after 24~48h
Enter formula bilayer bletilla striata dressing.
Wherein, a length of 10~200mm of above-mentioned flat plate mold groove, width are 10~200mm, a height of 4~12mm.
Due to using above technical scheme, preparation method of the invention for pigment in bletilla striata stem block soaking process together
Dissolution mixes with bletilla polysaccharide is difficult to the problem of separating, and increases ethyl alcohol before polysaccharide dissolution and leaches pigment process, utilizes we
The mentioned bletilla polysaccharide pure color of method, yield are improved.To shorten the time, simplify technique, to the bletilla striata stem block initially taken
Short time soaking and washing is carried out, surface impurity is removed.To economize on resources, before alcohol precipitation, first to the obtained bletilla polysaccharide of immersion
Solution is concentrated, and alcohol precipitation and then is repeatedly washed to sediment is obtained with ethyl alcohol, and moisture and the letter on bletilla polysaccharide surface are removed
Single monosaccharide molecule.
Between 3~10%, concentration determines the concentration of the bletilla polysaccharide casting solution prepared in preparation method of the present invention
Pore size, pore structure and the mechanical property of the lower layer bletilla striata dressing of preparation further influence dressing in wound healing process
In effect.Concentration is lower than 3% gained dressing poor mechanical property, and hole configurations is imperfect, easily collapses.When concentration is higher than 10%,
The dressing being prepared is partially hard, irritating to wound, uncomfortable, compact structure, is unfavorable for the exchange of inner air and outer air, extends wound
Healing time.Bletilla polysaccharide solution after being completely dissolved need to be placed under the vacuum state that vacuum degree is 0.1~0.5MPa and remove
Bubble, the pore structure after preventing the air impact dressing dissolved into solution to be finally freeze-dried.
The preparation of embeding layer casting solution, is realized by chemical modification method in preparation method of the present invention, i.e. preparation quality point
The sodium hydrate aqueous solution that number is 1~4%, takes the configured bletilla polysaccharide casting solution in part, dropwise by sodium hydrate aqueous solution
It is added in the bletilla polysaccharide casting solution under stirring, is removed the acetyl group in bletilla polysaccharide molecule using sodium hydroxide, from
And obtain embeding layer casting solution.
Using orientation ice crystal induced growth techinque bletilla polysaccharide casting solution and embeding layer are cast in preparation method of the present invention
Film liquid is sufficiently directional to freeze shaping membrane.Preparation method of the present invention is by dropping the metal transducer being arranged in plate groove
Temperature, poor in the one end and its corresponding end formation temperature for being equipped with metal transducer, the moisture in casting solution freezes, and cross growth is white
Splendid achnatherum polysaccharide molecule is precipitated from casting solution, is fixed in the ice phase gap of ice-crystal growth, obtains lower layer's bletilla polysaccharide film.It is preparing
During double-layer structure, first by bletilla polysaccharide casting solution freeze forming film, inverted T shaped groove is scribed on its surface, is put into mold, then
Embeding layer casting solution is poured on type film, embeding layer casting solution is full of on type film because of the gravity of itself and good mobile performance
Surface groove, and surface forms certain thickness liquid layer on it, and mold is transferred to heat-insulated container, pours into liquid nitrogen, embeding layer casting
Moisture freezes in film liquid, vertical to grow, and the low aqueous solubility bletilla polysaccharide molecule for removing acetyl group is fixed on the ice phase of ice-crystal growth
In gap, it is sufficiently directional freeze after form Embedded Double stratotype film, be placed in cryogenic vacuum state and go down moisture removal, obtain embedded
The double-deck bletilla striata dressing.Two interlayer of dressing forms due to the presence of cutting and is embedded in sealed structure, can be that the connection between two layers mentions
For enough mechanical strengths, meet the requirement in dressing use process to two interlayer mechanical properties.
The quantity of cutting determines the intensity connected between bilayer, the more intensity that can reduce lower layer's dressing of cutting quantity, no
Conducive to the connection between bilayer.Therefore, select the density of 1~2/10mm in the upper surface cutting of bletilla polysaccharide film.
The depth of cutting equally affects the compactness connected between upper layer.When groove depth is greater than 2mm, the lower layer bletilla striata
The mechanical property of dressing is damaged, and causes the mechanical properties decrease of monolith dressing serious, is unable to reach requirement.Work as cutting
When depth is less than 1mm, the chimeric defective tightness of two interlayers is easily slipped.Therefore, the groove depth of 1~2mm is selected.
The present invention prepares the double-deck bletilla striata dressing using cutting and freezing, and in conjunction with reliably, simple process overcomes the bletilla striata
Dressing soluble adhesion gloves, the difficult problem for being detached from docile wound when taking.Gained bletilla striata dressing of the invention in vivo can quilt
Degradation absorbs, good with biocompatible, without side-effects to wound, and orientation pore structure ensure that penetrating fluid is efficiently inhaled in dressing
It receives, while keeping dressing soft, wound will not be damaged.
Using the embedded double-deck bletilla striata dressing prepared by preparation method of the present invention, lower layer's bletilla polysaccharide oriented film, hole
Structure is conducive to the absorption to penetrating fluid, and the surface of a wound is dry, and wound is in wet environment, and bletilla polysaccharide is readily soluble, hemostatic bacteriostatic
Promoting healing effect can be played efficiently, excellent in mechanical performance, and lateral hole ensure that the flexibility of film, non-stimulated to wound.It is embedding
Enter a layer low aqueous solubility bletilla striata oriented film, ensure that convenience that doctor takes during surgery and dressing and gloves when to treatment of wounds
Easy disengaging solves the problems, such as that the adhesion difficulty that existing bletilla striata dressing faces in is detached from, dissolution wastes, it is more to have reached the bletilla striata
The sugared purpose efficiently utilized, vertical through-hole structure, so that embeding layer has toughness in the horizontal direction, vertically mould
Measure moderate, final dressing integrally obtains splendid mechanical property.
Specific embodiment
Present invention is further described in detail combined with specific embodiments below：
A kind of preparation method of the embedded double-deck bletilla striata dressing, the preparation method carry out according to the following steps：
The extraction of a bletilla polysaccharide
By dry bletilla striata stem block and distilled water with mass ratio 1:3~1:10 ratio is impregnated, and soaking time is 3~
20min, soaking temperature are 25~40 DEG C, and filtered through gauze removes solvable and not soluble in water impurity, collect the preliminary clean bletilla striata
Stem block is in 30~50 DEG C of oven dryings 18~for 24 hours.
Bletilla striata stem block and dehydrated alcohol after preliminary cleaning is dried is with mass ratio 1:3~1:10 ratio is impregnated, and is impregnated
Time is 1~3h, and soaking temperature is 20~30 DEG C, and filtered through gauze removes soluble pigment, collects bletilla striata stem block in 30~50 DEG C
Oven drying 18~for 24 hours.
Bletilla striata stem block and distilled water after removing depigmentaton is with mass ratio 1:3~1:10 ratio is impregnated, and soaking time is
0.5~3h, soaking temperature are 50~90 DEG C, and filtered through gauze collects bletilla polysaccharide filtrate.
By extract bletilla polysaccharide filtrate after bletilla striata stem block continue extract bletilla polysaccharide filtrate immersion and filter process 3~
10 times, the mass conservation of added distilled water merges the bletilla polysaccharide filtrate collected by several times, is placed in 30~80 DEG C of rotary evaporation
It is concentrated in instrument, obtains the bletilla polysaccharide concentrate that mass concentration is 1.5~5%.
Absolute ethyl alcohol and stirring is added into bletilla polysaccharide concentrate, alcohol precipitation is obtained by filtration precipitating, and is washed with dehydrated alcohol
The precipitating being obtained by filtration, wherein the volume of dehydrated alcohol added by alcohol precipitation is 1~2 times of the bletilla polysaccharide volume of the concentrated liquid, and washing is heavy
Starch number is 2~10 times, and each wash time is 1~5min, and the bletilla polysaccharide after washing is placed in baking oven to dry, drying
Temperature is 40~80 DEG C, and dry bletilla polysaccharide is made.
The preparation of b bletilla polysaccharide casting solution
Drying bletilla polysaccharide prepared by step a is dissolved in water and makes the bletilla polysaccharide that mass fraction is 3~10%
Solution is placed in the vacuum state that vacuum degree is 0.1~0.5MPa and goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of c sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 1~4%, is stirred evenly cold
But to room temperature.
The preparation of d embeding layer casting solution
The bletilla polysaccharide casting solution prepared in 20~50% step b is taken, sodium hydroxide solution prepared by step c is with 0.3
The speed of~1.0ml/min is added drop-wise in the bletilla polysaccharide casting solution stirred under 200~500r/min speed, stirs 2~5h
Afterwards, it is placed in 3~6h in the environment that vacuum degree is 0.05~0.1MPa and removes bubble removing, obtain embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 96:4~99:1.
E bilayer bletilla striata dressing preparation
Bletilla polysaccharide casting solution prepared in step b is poured into the groove of flat plate mold, the bottom of perpendicular in groove
It is provided with the metal transducer of transmitting temperature on one side in face, flat plate mold is wrapped up with heat-barrier material, by flat plate mold water
It lays flat in the environment for being placed in -50~-80 DEG C, freezes shaping membrane until the bletilla polysaccharide casting solution in flat plate mold is sufficiently directional,
Formed 3~8mm thickness bletilla polysaccharide film, type film is taken out, with sharp inverted T shaped slotting cutter type film upper surface with 1~2/
The density of cm scribes inverted T shaped groove, and the lower width of rebate of inverted T shaped groove is 1.5~3mm, and the width ratio of lower notch and upper notch is
2:1~3:1, the depth of inverted T shaped groove is 1~2mm, and upper notch and lower depth of rebate ratio are 1:1~1:2, upper notch is located at type
Type film cutting is put into the flat plate mold groove of above-mentioned not set metal transducer upwardly, is prepared by step d by film upper surface
Embeding layer casting solution pour into the upper layer for having carved the type film of slot, embeding layer casting solution is with volume ratio used in bletilla polysaccharide casting solution
0.2:1~1:1, layer casting solution to be embedded is full of cutting, entire mold is placed in heat-insulated container, liquid is poured into container
Nitrogen, liquid nitrogen upper surface are no more than cutting bottom surface and remove mold, the double deck type that will freeze after all solution are fully cured in mold
Film is placed in that temperature is -60~-80 DEG C, vacuum degree is to be freeze-dried in the environment of 1~5Pa, takes out, that is, obtains embedding after 24~48h
Enter formula bilayer bletilla striata dressing.
Wherein, a length of 10~200mm of above-mentioned flat plate mold groove, width are 10~200mm, a height of 4~12mm.
Specific embodiment
It is prepared as described above method
Embodiment one
The extraction of a bletilla polysaccharide
By dry bletilla striata stem block and distilled water with mass ratio 1:3 ratio is impregnated, soaking time 3min, soaking temperature
It is 25 DEG C, filtered through gauze removes solvable and not soluble in water impurity, collects preliminary clean bletilla striata stem block in 30 DEG C of oven dryings
24h。
Bletilla striata stem block and dehydrated alcohol after preliminary cleaning is dried is with mass ratio 1:6 ratio is impregnated, and soaking time is
1h, soaking temperature are 30 DEG C, and filtered through gauze removes soluble pigment, collect bletilla striata stem block in 50 DEG C of oven drying 18h.
Bletilla striata stem block and distilled water after removing depigmentaton is with mass ratio 1:3 ratio is impregnated, soaking time 3h, is impregnated
Temperature is 60 DEG C, and filtered through gauze collects bletilla polysaccharide filtrate.
Bletilla striata stem block after extraction bletilla polysaccharide filtrate is continued to immersion and the filter process 10 of extraction bletilla polysaccharide filtrate
Secondary, the mass conservation of added distilled water merges the bletilla polysaccharide filtrate collected by several times, is placed in dense in 80 DEG C of Rotary Evaporators
Contracting obtains the bletilla polysaccharide concentrate that mass concentration is 3%.
Absolute ethyl alcohol and stirring is added into bletilla polysaccharide concentrate, alcohol precipitation is obtained by filtration precipitating, and is washed with dehydrated alcohol
The precipitating being obtained by filtration, wherein the volume of dehydrated alcohol added by alcohol precipitation is 1 times of the bletilla polysaccharide volume of the concentrated liquid, washing precipitating
Object number is 7 times, and each wash time is 5min, and the bletilla polysaccharide after washing is placed in baking oven to dry, drying temperature 40
DEG C, dry bletilla polysaccharide is made.
The preparation of b bletilla polysaccharide casting solution
Drying bletilla polysaccharide prepared by step a is dissolved in water make mass fraction be 10% bletilla polysaccharide it is molten
Liquid is placed in the vacuum state that vacuum degree is 0.5MPa and goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of c sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 1%, stirs evenly cooling
To room temperature.
The preparation of d embeding layer casting solution
The bletilla polysaccharide casting solution prepared in 20% step b is taken, sodium hydroxide solution prepared by step c is with 0.3ml/
The speed of min is added drop-wise in the bletilla polysaccharide casting solution stirred under 500r/min speed, and after stirring 2h, being placed in vacuum degree is
6h removes bubble removing in the environment of 0.1MPa, obtains embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 96:4.
E bilayer bletilla striata dressing preparation
Bletilla polysaccharide casting solution prepared in step b is poured into the groove of flat plate mold, the bottom of perpendicular in groove
It is provided with the metal transducer of transmitting temperature on one side in face, flat plate mold is wrapped up with heat-barrier material, by flat plate mold water
It lays flat in the environment for being placed in -50 DEG C, freezes shaping membrane until the bletilla polysaccharide casting solution in flat plate mold is sufficiently directional, formed
The bletilla polysaccharide film of 3mm thickness, type film is taken out, and is carved with sharp inverted T shaped slotting cutter in type film upper surface with the density of 1/cm
Inverted T shaped groove processed, the lower width of rebate of inverted T shaped groove are 1.5mm, and the width ratio of lower notch and upper notch is 2:1, inverted T shaped groove
Depth is 1mm, and upper notch and lower depth of rebate ratio are 1:1, upper notch is located at type film upper surface, and type film cutting is put upwardly
In the flat plate mold groove for entering above-mentioned not set metal transducer, embeding layer casting solution prepared by step d is poured into and has carved slot
Volume ratio used in the upper layer of type film, embeding layer casting solution and bletilla polysaccharide casting solution is 0.5:1, layer casting solution to be embedded is full of quarter
Entire mold is placed in heat-insulated container by slot, and liquid nitrogen is poured into container, and liquid nitrogen upper surface is no more than cutting bottom surface, mold
After interior all solution are fully cured, mold is removed, it is the environment that -60, vacuum degree is 1Pa that the double deck type film freezed, which is placed in temperature,
Middle freeze-drying is taken out after 36h, that is, obtains the embedded double-deck bletilla striata dressing.
Wherein, a length of 10mm of above-mentioned flat plate mold groove, width 10mm, a height of 5mm.
Embodiment two
The extraction of a bletilla polysaccharide
By dry bletilla striata stem block and distilled water with mass ratio 1:5 ratio is impregnated, soaking time 10min, impregnates temperature
Degree is 25 DEG C, and filtered through gauze removes solvable and not soluble in water impurity, and it is dry in 30 DEG C of baking ovens to collect preliminary clean bletilla striata stem block
It is dry for 24 hours.
Bletilla striata stem block and dehydrated alcohol after preliminary cleaning is dried is with mass ratio 1:10 ratio is impregnated, and soaking time is
1h, soaking temperature are 30 DEG C, and filtered through gauze removes soluble pigment, collect bletilla striata stem block in 30 DEG C of oven dryings for 24 hours.
Bletilla striata stem block and distilled water after removing depigmentaton is with mass ratio 1:10 ratio is impregnated, soaking time 0.5h,
Soaking temperature is 90 DEG C, and filtered through gauze collects bletilla polysaccharide filtrate.
Bletilla striata stem block after extraction bletilla polysaccharide filtrate is continued to immersion and the filter process 3 of extraction bletilla polysaccharide filtrate
Secondary, the mass conservation of added distilled water merges the bletilla polysaccharide filtrate collected by several times, is placed in dense in 60 DEG C of Rotary Evaporators
Contracting obtains the bletilla polysaccharide concentrate that mass concentration is 1.5%.
Absolute ethyl alcohol and stirring is added into bletilla polysaccharide concentrate, alcohol precipitation is obtained by filtration precipitating, and is washed with dehydrated alcohol
The precipitating being obtained by filtration, wherein the volume of dehydrated alcohol added by alcohol precipitation is 2 times of the bletilla polysaccharide volume of the concentrated liquid, washing precipitating
Object number is 6 times, and each wash time is 5min, and the bletilla polysaccharide after washing is placed in baking oven to dry, drying temperature 50
DEG C, dry bletilla polysaccharide is made.
The preparation of b bletilla polysaccharide casting solution
Drying bletilla polysaccharide prepared by step a is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 6%,
It is placed in the vacuum state that vacuum degree is 0.2MPa to go down bubble removing, obtains bletilla polysaccharide casting solution, it is spare.
The preparation of c sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 2%, stirs evenly cooling
To room temperature.
The preparation of d embeding layer casting solution
The bletilla polysaccharide casting solution prepared in 30% step b is taken, sodium hydroxide solution prepared by step c is with 0.8ml/
The speed of min is added drop-wise in the bletilla polysaccharide casting solution stirred under 300r/min speed, and after stirring 4h, being placed in vacuum degree is
6h removes bubble removing in the environment of 0.06MPa, obtains embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 97:3.
E bilayer bletilla striata dressing preparation
Bletilla polysaccharide casting solution prepared in step b is poured into the groove of flat plate mold, the bottom of perpendicular in groove
It is provided with the metal transducer of transmitting temperature on one side in face, flat plate mold is wrapped up with heat-barrier material, by flat plate mold water
It lays flat in the environment for being placed in -60 DEG C, freezes shaping membrane until the bletilla polysaccharide casting solution in flat plate mold is sufficiently directional, formed
The bletilla polysaccharide film of 5mm thickness, type film is taken out, and is carved with sharp inverted T shaped slotting cutter in type film upper surface with the density of 1/cm
Inverted T shaped groove processed, the lower width of rebate of inverted T shaped groove are 3mm, and the width ratio of lower notch and upper notch is 3:1, the depth of inverted T shaped groove
Degree is 2mm, and upper notch and lower depth of rebate ratio are 1:1, upper notch is located at type film upper surface, and type film cutting is put into upwardly
In the flat plate mold groove of above-mentioned not set metal transducer, embeding layer casting solution prepared by step d is poured into the type for having carved slot
Volume ratio used in the upper layer of film, embeding layer casting solution and bletilla polysaccharide casting solution is 1:1, layer casting solution to be embedded is full of cutting,
Entire mold is placed in heat-insulated container, liquid nitrogen is poured into container, liquid nitrogen upper surface is no more than cutting bottom surface, institute in mold
After having solution to be fully cured, mold is removed, the double deck type film freezed is placed in temperature is -60 DEG C, vacuum degree is in the environment of 3Pa
It is freeze-dried, is taken out after 48h, that is, obtain the embedded double-deck bletilla striata dressing.
Wherein, a length of 100mm of above-mentioned flat plate mold groove, width 80mm, a height of 10mm.
Embodiment three
The extraction of a bletilla polysaccharide
By dry bletilla striata stem block and distilled water with mass ratio 1:6 ratio is impregnated, soaking time 15min, impregnates temperature
Degree is 30 DEG C, and filtered through gauze removes solvable and not soluble in water impurity, and it is dry in 45 DEG C of baking ovens to collect preliminary clean bletilla striata stem block
Dry 21h.
Bletilla striata stem block and dehydrated alcohol after preliminary cleaning is dried is with mass ratio 1:9 ratio is impregnated, and soaking time is
2h, soaking temperature are 30 DEG C, and filtered through gauze removes soluble pigment, collect bletilla striata stem block in 45 DEG C of oven drying 20h.
Bletilla striata stem block and distilled water after removing depigmentaton is with mass ratio 1:6 ratio is impregnated, soaking time 2.5h, leaching
Steeping temperature is 75 DEG C, and filtered through gauze collects bletilla polysaccharide filtrate.
Bletilla striata stem block after extraction bletilla polysaccharide filtrate is continued to immersion and the filter process 6 of extraction bletilla polysaccharide filtrate
Secondary, the mass conservation of added distilled water merges the bletilla polysaccharide filtrate collected by several times, is placed in dense in 40 DEG C of Rotary Evaporators
Contracting obtains the bletilla polysaccharide concentrate that mass concentration is 1.5%.
Absolute ethyl alcohol and stirring is added into bletilla polysaccharide concentrate, alcohol precipitation is obtained by filtration precipitating, and is washed with dehydrated alcohol
The precipitating being obtained by filtration, wherein the volume of dehydrated alcohol added by alcohol precipitation is 1.2 times of the bletilla polysaccharide volume of the concentrated liquid, and washing is heavy
Starch number is 4 times, and each wash time is 3min, and the bletilla polysaccharide after washing is placed in drying, drying temperature in baking oven and is
55 DEG C, dry bletilla polysaccharide is made.
The preparation of b bletilla polysaccharide casting solution
Drying bletilla polysaccharide prepared by step a is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 7%,
It is placed in the vacuum state that vacuum degree is 0.4MPa to go down bubble removing, obtains bletilla polysaccharide casting solution, it is spare.
The preparation of c sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 3%, stirs evenly cooling
To room temperature.
The preparation of d embeding layer casting solution
The bletilla polysaccharide casting solution prepared in 40% step b is taken, sodium hydroxide solution prepared by step c is with 0.6ml/
The speed of min is added drop-wise in the bletilla polysaccharide casting solution stirred under 300r/min speed, and after stirring 3h, being placed in vacuum degree is
4.5h removes bubble removing in the environment of 0.08MPa, obtains embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 98:2.
E bilayer bletilla striata dressing preparation
Bletilla polysaccharide casting solution prepared in step b is poured into the groove of flat plate mold, the bottom of perpendicular in groove
It is provided with the metal transducer of transmitting temperature on one side in face, flat plate mold is wrapped up with heat-barrier material, by flat plate mold water
It lays flat in the environment for being placed in -75 DEG C, freezes shaping membrane until the bletilla polysaccharide casting solution in flat plate mold is sufficiently directional, formed
The bletilla polysaccharide film of 6mm thickness, type film is taken out, and is carved with sharp inverted T shaped slotting cutter in type film upper surface with the density of 2/cm
Inverted T shaped groove processed, the lower width of rebate of inverted T shaped groove are 1.5mm, and the width ratio of lower notch and upper notch is 3:1, inverted T shaped groove
Depth is 2mm, and upper notch and lower depth of rebate ratio are 1:2, upper notch is located at type film upper surface, and type film cutting is put upwardly
In the flat plate mold groove for entering above-mentioned not set metal transducer, embeding layer casting solution prepared by step d is poured into and has carved slot
Volume ratio used in the upper layer of type film, embeding layer casting solution and bletilla polysaccharide casting solution is 0.4:1, layer casting solution to be embedded is full of quarter
Entire mold is placed in heat-insulated container by slot, and liquid nitrogen is poured into container, and liquid nitrogen upper surface is no more than cutting bottom surface, mold
After interior all solution are fully cured, mold is removed, the double deck type film freezed is placed in the ring that temperature is -75 DEG C, vacuum degree is 3Pa
It is freeze-dried in border, is taken out after 26h, that is, obtain the embedded double-deck bletilla striata dressing.
Wherein, a length of 200mm of above-mentioned flat plate mold groove, width 200mm, a height of 10mm.
Example IV
The extraction of a bletilla polysaccharide
By dry bletilla striata stem block and distilled water with mass ratio 1:9.5 ratio is impregnated, soaking time 3min, impregnates temperature
Degree is 30 DEG C, and filtered through gauze removes solvable and not soluble in water impurity, and it is dry in 45 DEG C of baking ovens to collect preliminary clean bletilla striata stem block
Dry 23h.
Bletilla striata stem block and dehydrated alcohol after preliminary cleaning is dried is with mass ratio 1:7 ratio is impregnated, and soaking time is
2.5h, soaking temperature are 30 DEG C, and filtered through gauze removes soluble pigment, collect bletilla striata stem block in 50 DEG C of oven drying 20h.
Bletilla striata stem block and distilled water after removing depigmentaton is with mass ratio 1:6 ratio is impregnated, soaking time 1.5h, leaching
Steeping temperature is 65 DEG C, and filtered through gauze collects bletilla polysaccharide filtrate.
Bletilla striata stem block after extraction bletilla polysaccharide filtrate is continued to immersion and the filter process 5 of extraction bletilla polysaccharide filtrate
Secondary, the mass conservation of added distilled water merges the bletilla polysaccharide filtrate collected by several times, is placed in dense in 60 DEG C of Rotary Evaporators
Contracting obtains the bletilla polysaccharide concentrate that mass concentration is 2.5%.
Absolute ethyl alcohol and stirring is added into bletilla polysaccharide concentrate, alcohol precipitation is obtained by filtration precipitating, and is washed with dehydrated alcohol
The precipitating being obtained by filtration, wherein the volume of dehydrated alcohol added by alcohol precipitation is 2 times of the bletilla polysaccharide volume of the concentrated liquid, washing precipitating
Object number is 10 times, and each wash time is 4.5min, and the bletilla polysaccharide after washing is placed in drying, drying temperature in baking oven and is
70 DEG C, dry bletilla polysaccharide is made.
The preparation of b bletilla polysaccharide casting solution
Drying bletilla polysaccharide prepared by step a is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 8%,
It is placed in the vacuum state that vacuum degree is 0.3MPa to go down bubble removing, obtains bletilla polysaccharide casting solution, it is spare.
The preparation of c sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 4%, stirs evenly cooling
To room temperature.
The preparation of d embeding layer casting solution
The bletilla polysaccharide casting solution prepared in 45% step b is taken, sodium hydroxide solution prepared by step c is with 1.0ml/
The speed of min is added drop-wise in the bletilla polysaccharide casting solution stirred under 500r/min speed, and after stirring 4h, being placed in vacuum degree is
4.5h removes bubble removing in the environment of 0.1MPa, obtains embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 99:1.
E bilayer bletilla striata dressing preparation
Bletilla polysaccharide casting solution prepared in step b is poured into the groove of flat plate mold, the bottom of perpendicular in groove
It is provided with the metal transducer of transmitting temperature on one side in face, flat plate mold is wrapped up with heat-barrier material, by flat plate mold water
It lays flat in the environment for being placed in -80 DEG C, freezes shaping membrane until the bletilla polysaccharide casting solution in flat plate mold is sufficiently directional, formed
The bletilla polysaccharide film of 8mm thickness, type film is taken out, and is carved with sharp inverted T shaped slotting cutter in type film upper surface with the density of 2/cm
Inverted T shaped groove processed, the lower width of rebate of inverted T shaped groove are 2.4mm, and the width ratio of lower notch and upper notch is 2:1, inverted T shaped groove
Depth is 2mm, and upper notch and lower depth of rebate ratio are 1:1, upper notch is located at type film upper surface, and type film cutting is put upwardly
In the flat plate mold groove for entering above-mentioned not set metal transducer, embeding layer casting solution prepared by step d is poured into and has carved slot
Volume ratio used in the upper layer of type film, embeding layer casting solution and bletilla polysaccharide casting solution is 0.5:1, layer casting solution to be embedded is full of quarter
Entire mold is placed in heat-insulated container by slot, and liquid nitrogen is poured into container, and liquid nitrogen upper surface is no more than cutting bottom surface, mold
After interior all solution are fully cured, mold is removed, the double deck type film freezed is placed in the ring that temperature is -80 DEG C, vacuum degree is 4Pa
It is freeze-dried in border, is taken out after 48h, that is, obtain the embedded double-deck bletilla striata dressing.
Wherein, a length of 200mm of above-mentioned flat plate mold groove, width 150mm, a height of 12mm.
Claims (1)
1. a kind of preparation method of the embedded double-deck bletilla striata dressing, which is characterized in that preparation method carries out according to the following steps：
The extraction of a bletilla polysaccharide
By dry bletilla striata stem block and distilled water with mass ratio 1:3~1:10 ratio is impregnated, and soaking time is 3~20min, leaching
Steeping temperature is 25~40 DEG C, and filtered through gauze removes solvable and not soluble in water impurity, collects preliminary clean bletilla striata stem block in 30
~50 DEG C of oven dryings 18~for 24 hours；
Bletilla striata stem block and dehydrated alcohol after preliminary cleaning is dried is with mass ratio 1:3~1:10 ratio is impregnated, soaking time
For 1~3h, soaking temperature is 20~30 DEG C, and filtered through gauze removes soluble pigment, collects bletilla striata stem block in 30~50 DEG C of baking ovens
Dry 18~for 24 hours；
Bletilla striata stem block and distilled water after removing depigmentaton is with mass ratio 1:3~1:10 ratio is impregnated, and soaking time is 0.5~
3h, soaking temperature are 50~90 DEG C, and filtered through gauze collects bletilla polysaccharide filtrate；
Bletilla striata stem block after extraction bletilla polysaccharide filtrate is continued to immersion and the filter process 3~10 of extraction bletilla polysaccharide filtrate
Secondary, the mass conservation of added distilled water merges the bletilla polysaccharide filtrate collected by several times, is placed in 30~80 DEG C of Rotary Evaporators
Middle concentration obtains the bletilla polysaccharide concentrate that mass concentration is 1.5~5%；
Absolute ethyl alcohol and stirring is added into the bletilla polysaccharide concentrate of acquisition, alcohol precipitation is obtained by filtration precipitating, and is washed with dehydrated alcohol
Wash the precipitating being obtained by filtration, wherein the volume of dehydrated alcohol added by alcohol precipitation is 1~2 times of the bletilla polysaccharide volume of the concentrated liquid, washing
Sediment number is 2~10 times, and each wash time is 1~5min, and the bletilla polysaccharide after washing is placed in drying in baking oven, is done
Dry temperature is 40~80 DEG C, and dry bletilla polysaccharide is made；
The preparation of b bletilla polysaccharide casting solution
Drying bletilla polysaccharide prepared by step a is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 3~10%,
It is placed in the vacuum state that vacuum degree is 0.1~0.5MPa to go down bubble removing, obtains bletilla polysaccharide casting solution, it is spare；
The preparation of c sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 1~4%, stirs evenly and is cooled to
Room temperature；
The preparation of d embeding layer casting solution
Take the bletilla polysaccharide casting solution prepared in 20~50% step b, sodium hydroxide solution prepared by step c with 0.3~
The speed of 1.0ml/min is added drop-wise in the bletilla polysaccharide casting solution stirred under 200~500r/min speed, after stirring 2~5h,
It is placed in 3~6h in the environment that vacuum degree is 0.05~0.1MPa and removes bubble removing, obtain embeding layer casting solution；
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 96:4~99:1；
E bilayer bletilla striata dressing preparation
Bletilla polysaccharide casting solution prepared in step b is poured into the groove of flat plate mold, perpendicular to bottom surface in groove
It is provided with the metal transducer of transmitting temperature on one side, wraps up flat plate mold with heat-barrier material, flat plate mold level is put
It is placed in -50~-80 DEG C of environment, freezes shaping membrane until the bletilla polysaccharide casting solution in flat plate mold is sufficiently directional, form 3
The bletilla polysaccharide film of~8mm thickness, type film is taken out, with sharp inverted T shaped slotting cutter in type film upper surface with the close of 1~2/cm
Degree scribes inverted T shaped groove, and the lower width of rebate of inverted T shaped groove is 1.5~3mm, and the width ratio of lower notch and upper notch is 2:1~3:
1, the depth of inverted T shaped groove is 1~2mm, and upper notch and lower depth of rebate ratio are 1:1~1:2, upper notch is located at table on type film
Type film cutting is put into the flat plate mold groove of above-mentioned not set metal transducer upwardly, insertion prepared by step d by face
Layer casting solution pours on the type film for having carved slot, and volume ratio used in embeding layer casting solution and bletilla polysaccharide casting solution is 0.2:1~1:
1, layer casting solution to be embedded is full of cutting, entire mold is placed in heat-insulated container, liquid nitrogen, table on liquid nitrogen are poured into container
Face is no more than cutting bottom surface and removes mold after all solution are fully cured in mold, the double deck type film freezed is placed in temperature
It is to be freeze-dried in the environment of 1~5Pa for -60~-80 DEG C, vacuum degree, is taken out after 24~48h, that is, it is white obtains embedded bilayer
Splendid achnatherum dressing.
Priority Applications (2)
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|---|---|---|---|
| CN201810851657.7A CN108904867A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of the embedded double-deck bletilla striata dressing |
| CN201910650588.8A CN110215535B (en) | 2018-07-27 | 2019-07-18 | Preparation method of bletilla striata dressing with embedded structural formula |
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| CN201810851657.7A CN108904867A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of the embedded double-deck bletilla striata dressing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112494707A (en) * | 2020-10-30 | 2021-03-16 | 华熙生物科技股份有限公司 | Wound hemostasis repair product based on hyaluronic acid and preparation method thereof |
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2018
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112494707A (en) * | 2020-10-30 | 2021-03-16 | 华熙生物科技股份有限公司 | Wound hemostasis repair product based on hyaluronic acid and preparation method thereof |
| CN112494707B (en) * | 2020-10-30 | 2022-05-27 | 华熙生物科技股份有限公司 | Wound hemostasis repair product based on hyaluronic acid and preparation method thereof |
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