CN110201209B - Preparation method of porous material reinforced bletilla striata dressing - Google Patents
Preparation method of porous material reinforced bletilla striata dressing Download PDFInfo
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- CN110201209B CN110201209B CN201910651318.9A CN201910651318A CN110201209B CN 110201209 B CN110201209 B CN 110201209B CN 201910651318 A CN201910651318 A CN 201910651318A CN 110201209 B CN110201209 B CN 110201209B
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- bletilla striata
- solution
- striata polysaccharide
- dressing
- polysaccharide
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- 241001313857 Bletilla striata Species 0.000 title claims abstract description 231
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 239000011148 porous material Substances 0.000 title claims abstract description 44
- 150000004676 glycans Chemical class 0.000 claims abstract description 163
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 163
- 239000005017 polysaccharide Substances 0.000 claims abstract description 163
- 238000005266 casting Methods 0.000 claims abstract description 79
- 239000012528 membrane Substances 0.000 claims abstract description 37
- 229920002201 Oxidized cellulose Polymers 0.000 claims abstract description 30
- 229940107304 oxidized cellulose Drugs 0.000 claims abstract description 30
- 239000004020 conductor Substances 0.000 claims abstract description 29
- 239000002184 metal Substances 0.000 claims abstract description 29
- 108010010803 Gelatin Proteins 0.000 claims abstract description 27
- 229920000159 gelatin Polymers 0.000 claims abstract description 27
- 239000008273 gelatin Substances 0.000 claims abstract description 27
- 235000019322 gelatine Nutrition 0.000 claims abstract description 27
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000007710 freezing Methods 0.000 claims abstract description 15
- 230000008014 freezing Effects 0.000 claims abstract description 15
- 230000009471 action Effects 0.000 claims abstract description 12
- 238000004108 freeze drying Methods 0.000 claims abstract description 9
- 239000004744 fabric Substances 0.000 claims description 3
- 238000002844 melting Methods 0.000 claims description 3
- 230000008018 melting Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 14
- 230000007480 spreading Effects 0.000 abstract description 5
- 238000003892 spreading Methods 0.000 abstract description 5
- 229940079593 drug Drugs 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000037314 wound repair Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 159
- 208000027418 Wounds and injury Diseases 0.000 description 21
- 206010052428 Wound Diseases 0.000 description 20
- 241001313855 Bletilla Species 0.000 description 11
- 230000002439 hemostatic effect Effects 0.000 description 11
- 238000002156 mixing Methods 0.000 description 7
- 239000008280 blood Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000035876 healing Effects 0.000 description 3
- 238000009941 weaving Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 231100000957 no side effect Toxicity 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000009987 spinning Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 239000000515 collagen sponge Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000032798 delamination Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000009940 knitting Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000021715 photosynthesis, light harvesting Effects 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/62—Compostable, hydrosoluble or hydrodegradable materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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- Health & Medical Sciences (AREA)
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- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention relates to a preparation method of a porous material reinforced bletilla striata dressing, and belongs to the technical field of biological medicines. The preparation method comprises the steps of directionally freezing part of bletilla striata polysaccharide membrane casting solution, pouring a connection layer bletilla striata polysaccharide solution on the upper part of the incompletely frozen bletilla striata polysaccharide membrane casting solution, spreading water-insoluble gelatin sponge or medical oxidized cellulose gauze above the melted solution, freezing a porous material, the connection layer bletilla striata polysaccharide solution and the bletilla striata polysaccharide membrane casting solution into a whole under the action of a metal conductor, and obtaining the porous material reinforced bletilla striata dressing by a freeze drying technology, so that the problems that the existing bletilla striata dressing is easy to dissolve in water and difficult to be pasted on a wound in clinical medical use are solved. The materials adopted by the preparation method of the invention can be degraded and absorbed, and are harmless to human body. The preparation method of the invention enhances the mechanical property and the operational performance of the bletilla striata dressing, and leads the application of the bletilla striata dressing in the fields of surgical operation, wound repair, tissue engineering and the like to be popularized.
Description
Technical Field
The invention relates to a preparation method of a porous material reinforced bletilla striata dressing, and belongs to the technical field of biological medicines.
Background
In surgical clinical use, the rapid hemostasis of wounds has an important effect on the postoperative recovery of patients. The bletilla striata polysaccharide has the functions of resisting bacteria and diminishing inflammation, removing putrefaction and promoting tissue regeneration, astringing sores and the like, has no toxic or side effect, is safe and comfortable, degradable, pollution-free and good in biocompatibility, is a good natural biological engineering material, and can be applied to pharmaceutical preparations as a drug dressing.
The invention discloses a method for using bletilla striata polysaccharide to stanch materials, wherein the Chinese patent publication No. is CN102477102A, the publication date is 2012/05/12, the name of the invention is that bletilla striata polysaccharide coarse powder extracted from bletilla striata tubers is prepared into bletilla striata polysaccharide aqueous solution, the bletilla striata polysaccharide aqueous solution is treated under low temperature and vacuum environment to prepare spongy materials, the adhesion positioning property and the fixing force of bleeding tissue parts are enhanced, and the application field of the bletilla striata polysaccharide is further expanded. But the inner structure design of the sponge material is not fine enough, and the capability of absorbing the penetrating fluid is poor. The Chinese patent publication number is CN102961776A, the publication date is 2013, 03 and 13, and the invention name is: a method for preparing bletilla striata hemostatic gauze comprises the steps of extracting bletilla striata gum from bletilla striata stem blocks by a water extraction and alcohol precipitation method, dissolving the bletilla striata gum in water to prepare a bletilla striata gum spinning solution, spinning the bletilla striata gum into filaments by taking absolute ethyl alcohol as a coagulating bath, weaving the filaments into gauze by a knitting, weaving or non-weaving method, cutting the gauze, sterilizing and packaging the gauze to obtain the water-soluble hemostatic gauze. The hemostatic gauze prepared by the invention is composed of the bletilla striata filaments, can be degraded and absorbed in vivo, has good biocompatibility, no side effect on wounds, good antibacterial, anti-inflammatory and rapid hemostatic effects, and improves the wound healing speed. However, the bletilla striata polysaccharide is very soluble in water, easy to adhere and difficult to separate in operation, and cannot be well attached to a wound in use.
The existing bletilla striata dressing can be dissolved and adhered due to the fact that blood is stained on gloves worn by doctors when the bletilla striata dressing is used in a surgical operation, and the bletilla striata dressing is difficult to be adhered to wounds at one time. The conventional multilayer dressing operation layer is not degradable, and the application range of the dressing is limited to a great extent.
Disclosure of Invention
In view of the existing problems, the invention aims to provide a preparation method of a porous material reinforced bletilla striata dressing.
In order to achieve the purpose, the technical solution of the invention is as follows:
a preparation method of a porous material reinforced bletilla striata dressing comprises the following steps:
a preparation of bletilla striata polysaccharide membrane casting solution
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 3-10%, and removing air bubbles under the vacuum condition of 0.1-0.5 MPa of vacuum degree to obtain a bletilla striata polysaccharide membrane casting solution for later use.
b preparation of bletilla striata polysaccharide solution of connecting layer
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 10-15%, and removing air bubbles in a vacuum state with the vacuum degree of 0.1-0.5 MPa to obtain a bletilla striata polysaccharide solution of a connecting layer.
c preparation of porous material reinforced bletilla striata dressing
Pouring the bletilla striata polysaccharide casting solution prepared in the step a into a groove of a flat plate die, arranging a metal conductor for transferring temperature on the bottom surface of the groove of the flat plate die, wherein the temperature of the metal conductor is-20 to-80 ℃, transferring the temperature from the bottom of the die upwards through the casting solution, when the total amount of the bletilla striata polysaccharide casting solution in the flat plate die is directionally frozen by 80 to 90 percent, pouring the bletilla striata polysaccharide solution of the connecting layer prepared in the step b into the upper part of the bletilla striata polysaccharide casting solution which is not completely frozen, melting the two solutions, distributing and flatly paving gelatin sponge or medical oxidized cellulose yarn which is cut into a shape with the same length and width as the groove of the flat plate die and the thickness of 1 to 3mm above the melted solution until the gelatin sponge or the medical oxidized cellulose yarn is completely soaked, and freezing the bletilla striata polysaccharide casting solution, the bletilla striata polysaccharide solution of the connecting layer and the gelatin sponge or the medical oxidized cellulose yarn cloth in the die into a whole under the action of the metal conductor, forming a porous material reinforced bletilla striata dressing type film with the thickness of 3-8 mm, placing the bletilla striata dressing type film in an environment with the temperature of-10 to-80 ℃ and the vacuum degree of 1-20 Pa for freeze drying, and taking out after 24-72 h to obtain the porous material reinforced bletilla striata dressing.
Wherein the mass ratio of the bletilla striata polysaccharide solution of the connecting layer to the bletilla striata polysaccharide casting solution is 0.2: 1-0.5: 1, the warp and weft density of the medical oxidized cellulose gauze is 10-50 threads/in, and the fineness of the threads in the gauze is 30-60 tex.
The length of the flat plate mold groove is 10-200 mm, the width is 10-200 mm, and the height is 3-12 mm.
Due to the adoption of the technical scheme, the preparation method of the invention introduces two kinds of medical hemostatic materials which are insoluble in water as the operation layers of the bletilla striata dressing, aiming at the problems that the existing bletilla striata dressing is easily dissolved in blood water during taking, adhesive gloves are difficult to fall off, and the wound can not be quickly and effectively covered. After the medical hemostatic material operation layer of difficult to dissolve in water that introduces on tuber of hyacinth bletilla dressing upper portion, take this dressing as the doctor, the medical hemostatic material that at first difficult to dissolve contacts with the hand that the doctor took blood, can not arouse foretell dissolution adhesion phenomenon, and the doctor of being convenient for has promoted the efficiency of wound treatment and healing to the processing of wound. The two medical hemostatic materials are gelatin sponge and medical oxidized cellulose gauze respectively, have excellent mechanical properties and good biocompatibility, can be degraded and absorbed in vivo, have no side effect on wounds, and are mature in clinical use. The medical oxidized cellulose gauze is soft, does not stimulate the wound, has good air permeability and is beneficial to the rapid healing of the wound. The gelatin sponge belongs to protein collagen sponge, and has good biocompatibility, hemostatic performance, new cell formation promoting function and cell adhesion.
According to the preparation method, when the bletilla striata polysaccharide membrane casting solution is not completely frozen and 10-20% of the bletilla striata polysaccharide membrane casting solution is still reserved on the upper portion, a connection layer bletilla striata polysaccharide solution is added, the two solutions are blended, the operation layer porous medical hemostatic material is flatly laid above the blended solution, the porous medical hemostatic material, the connection layer bletilla striata polysaccharide solution and the bletilla striata polysaccharide membrane casting solution are frozen into a whole by adopting a directional freezing technology, and the porous material reinforced bletilla striata dressing is prepared by combining a freezing and drying technology. The bletilla striata dressing prepared by the preparation method disclosed by the invention is soft in texture, and does not cause irritation to a wound, the mechanical property of the bletilla striata dressing is enhanced due to the addition of the porous material, the taking and the use of a doctor during wound treatment are facilitated, and the wound treatment efficiency is improved.
The amount of the bletilla striata polysaccharide membrane casting solution in the directional freezing flat plate die needs to consider the effect of the bletilla striata dressing and the bonding strength of the porous material and the bletilla striata dressing. When the amount of the frozen bletilla striata polysaccharide membrane casting solution is less than 80%, the amount of the bletilla striata polysaccharide membrane casting solution forming a bletilla striata dressing layer is reduced, and the bletilla striata dressing is not fully utilized; when the amount of the frozen bletilla striata polysaccharide membrane casting solution is higher than 90%, the amount of the connection layer bletilla striata polysaccharide solution blended with the unfrozen part of the bletilla striata polysaccharide membrane casting solution in a solution state is too small, the bonding strength of the porous material and the bletilla striata dressing is reduced, and the delamination phenomenon is easily caused.
The concentration of the bletilla striata polysaccharide solution at the connecting layer needs to be selected in consideration of the supporting performance of the solution obtained after the bletilla striata polysaccharide solution at the connecting layer is blended with the unfrozen bletilla striata polysaccharide casting solution on the porous material and the preparation efficiency of the bletilla striata polysaccharide solution at the connecting layer. When the concentration is lower than 10%, the support capability of the solution after the blending on the porous material is low, the upper surface of the porous material cannot be in the same horizontal plane with the upper surface of the solution after the blending, so that the porous material sinks, and the functions of preventing the dressing from dissolving and preventing the dressing from being adhered to an operation surface cannot be exerted; when the concentration is higher than 15%, the preparation period of the solution is long, and the preparation efficiency of the bletilla striata dressing is seriously influenced.
The bletilla striata dressing prepared by the invention has the advantages that the porous structure obtained by the bletilla striata dressing layer under the directional freezing action is beneficial to fully exerting the effect of quickly stopping bleeding of bletilla striata polysaccharide, and the bletilla striata dressing can be quickly dissolved in blood at a wound to form a protective film after being pasted on the wound to bind the blood inside the wound. Meanwhile, the bletilla striata polysaccharide has the effects of bacteriostasis and healing promotion. The porous material reinforced bletilla striata dressing effectively solves the problems that the existing bletilla striata dressing is easy to dissolve in water and difficult to be pasted on a wound in clinical medical use. The preparation method provided by the invention is simple in process and low in cost, and the two materials are firmly fixed together by utilizing the interpenetration and locking of the holes between the porous material and the bletilla striata dressing, so that the sufficient mechanical strength in use is ensured. The materials adopted by the preparation method of the invention can be degraded and absorbed, and are harmless to human body.
The thickness of the gelatin sponge or the medical oxidized cellulose gauze determines the quality of the anti-adhesion effect of the dressing, when the thickness is less than 1mm, water or blood can enter the bletilla striata dressing layer through holes due to the existence of the holes in the gelatin sponge and the medical oxidized cellulose gauze, so that a large amount of bletilla striata dressing is dissolved, and the gelatin sponge or the medical oxidized cellulose gauze can still be adhered; when the thickness is more than 3mm, on one hand, the waste of materials is caused, and on the other hand, the flexibility is reduced because the operation layer is too thick, and the stimulation is caused to the wound. Therefore, the thickness is selected to be 1 to 3 mm.
The metal conductor temperature is selected with consideration of efficiency and energy consumption. When the temperature is higher than-20 ℃, the bletilla polysaccharide casting solution and the bletilla polysaccharide solution of the connecting layer are slowly frozen under the action of the metal conductor, the use time is too long, and the efficiency is low; when the temperature is lower than minus 80 ℃, under the action of a metal conductor, the bletilla polysaccharide membrane casting solution and the bletilla polysaccharide solution on the connecting layer can be quickly frozen into a whole, but the energy dissipation is serious, and the energy conservation is not facilitated. Therefore, the temperature of the metal conductor is selected to be-20 to-80 ℃, and the bletilla striata polysaccharide casting solution and the bletilla striata polysaccharide solution of the connecting layer are frozen directionally.
The manufacturing method of the bletilla striata dressing avoids secondary injury to a wound caused by replacement of a traditional dressing, solves the problem that a doctor is inconvenient to operate when taking the bletilla striata dressing to paste the wound, enhances the mechanical property and the operation property of the bletilla striata dressing, and promotes application of the bletilla striata dressing in the fields of surgical operation, wound repair, tissue engineering and the like.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
A preparation method of a porous material reinforced bletilla striata base dressing comprises the following steps:
a preparation of bletilla striata polysaccharide membrane casting solution
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 3-10%, and removing air bubbles under the vacuum condition of 0.1-0.5 MPa of vacuum degree to obtain a bletilla striata polysaccharide membrane casting solution for later use.
b preparation of bletilla striata polysaccharide solution of connecting layer
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 10-15%, and removing air bubbles in a vacuum state with the vacuum degree of 0.1-0.5 MPa to obtain a bletilla striata polysaccharide solution of a connecting layer.
c preparation of porous material reinforced bletilla striata dressing
Pouring the bletilla striata polysaccharide casting solution prepared in the step a into a groove of a flat plate die, arranging a metal conductor for transferring temperature on the bottom surface of the groove of the flat plate die, wherein the temperature of the metal conductor is-20 to-80 ℃, transferring the temperature from the bottom of the die upwards through the casting solution, when the total amount of the bletilla striata polysaccharide casting solution in the flat plate die is directionally frozen by 80 to 90 percent, pouring the bletilla striata polysaccharide solution of the connecting layer prepared in the step b into the upper part of the bletilla striata polysaccharide casting solution which is not completely frozen, melting the two solutions, distributing and flatly paving gelatin sponge or medical oxidized cellulose yarn which is cut into a shape with the same length and width as the groove of the flat plate die and the thickness of 1 to 3mm above the melted solution until the gelatin sponge or the medical oxidized cellulose yarn is completely soaked, and freezing the bletilla striata polysaccharide casting solution, the bletilla striata polysaccharide solution of the connecting layer and the gelatin sponge or the medical oxidized cellulose yarn cloth in the die into a whole under the action of the metal conductor, forming a porous material reinforced bletilla striata dressing type film with the thickness of 3-8 mm, placing the bletilla striata dressing type film in an environment with the temperature of-10 to-80 ℃ and the vacuum degree of 1-20 Pa for freeze drying, and taking out after 24-72 h to obtain the porous material reinforced bletilla striata dressing.
The mass ratio of the bletilla striata polysaccharide solution of the connecting layer to the bletilla striata polysaccharide membrane casting solution is 0.2: 1-0.5: 1, the gelatin sponge can be obtained by adopting Chinese patent publication No. CN101574539A, the invention name is gelatin sponge and a preparation method thereof, the warp and weft density of the medical oxidized cellulose gauze is 10-50 pieces/in, and the yarn fineness in the gauze is 30-60 tex.
The length of the flat plate mold groove is 10-200 mm, the width is 10-200 mm, and the height is 3-12 mm.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The preparation method is as above
Example one
a preparation of bletilla striata polysaccharide membrane casting solution
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 3%, and placing the solution in a vacuum state with the vacuum degree of 0.1MPa to remove air bubbles to obtain a bletilla striata polysaccharide membrane casting solution for later use.
b preparation of bletilla striata polysaccharide solution of connecting layer
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 10%, and placing the solution in a vacuum state with the vacuum degree of 0.1MPa to remove air bubbles to obtain a bletilla striata polysaccharide solution of a connecting layer.
c preparation of porous material reinforced bletilla striata dressing
Pouring the bletilla striata polysaccharide casting solution prepared in the step a into a groove of a flat plate die, arranging a metal conductor for transferring temperature on the bottom surface of the groove of the flat plate die, wherein the temperature of the metal conductor is-20 ℃, transferring the temperature upwards from the bottom of the die through the casting solution, and when the 80 percent of the total weight of the bletilla striata polysaccharide casting solution in the flat plate die is directionally frozen, pouring the bletilla striata polysaccharide solution of the connecting layer prepared in the step b into the upper part of the bletilla striata polysaccharide casting solution which is not completely frozen, blending the two solutions, horizontally paving a gelatin sponge with the same length and width as the groove of the flat plate die and the thickness of 1mm above the blended solution until the gelatin sponge is completely soaked, freezing the bletilla striata polysaccharide casting solution, the bletilla striata polysaccharide solution of the connecting layer and the gelatin sponge into a whole under the action of the metal conductor to form a porous material reinforced bletilla striata dressing film with the thickness of 3mm, and placing the bletilla striata dressing film at, Freeze-drying in an environment with a vacuum degree of 1Pa, and taking out after 24h to obtain the porous material reinforced bletilla striata dressing.
Wherein, the mass ratio of the bletilla striata polysaccharide solution of the connecting layer to the bletilla striata polysaccharide membrane casting solution is 0.2:1, and the gelatin sponge can be obtained by adopting Chinese patent publication No. CN101574539A with the name of invention being a gelatin sponge and a preparation method thereof.
Wherein, the length of the flat plate mould groove is 10mm, the width is 10mm, and the height is 3 mm.
Example two
a preparation of bletilla striata polysaccharide membrane casting solution
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 5%, and placing the solution in a vacuum state with the vacuum degree of 0.2MPa to remove air bubbles to obtain a bletilla striata polysaccharide membrane casting solution for later use.
b preparation of bletilla striata polysaccharide solution of connecting layer
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 13%, and placing the solution in a vacuum state with the vacuum degree of 0.3MPa to remove air bubbles to obtain a bletilla striata polysaccharide solution of a connecting layer.
c preparation of porous material reinforced bletilla striata dressing
Pouring the bletilla striata polysaccharide casting solution prepared in the step a into a groove of a flat plate die, arranging a metal conductor for transferring temperature on the bottom surface of the groove of the flat plate die, pouring the bletilla striata polysaccharide solution of the connecting layer prepared in the step b into the upper part of the bletilla striata polysaccharide casting solution which is not completely frozen when the temperature of the metal conductor is-30 ℃, transferring the temperature from the bottom of the die upwards through the casting solution, and directionally freezing 85 percent of the total weight of the bletilla striata polysaccharide casting solution in the flat plate die, blending the two solutions, spreading medical oxidized cellulose yarn which is cut into a length, a width and a thickness which are the same as those of the groove of the flat plate die and is 1mm above the blended solution until the medical oxidized cellulose yarn is completely soaked, and freezing the polysaccharide bletilla striata casting solution, the bletilla striata polysaccharide solution of the connecting layer and the medical oxidized cellulose yarn into a whole under the action of the metal conductor to form a porous material reinforced bletilla striata dressing film with the thickness of 3mm, freeze drying rhizoma bletilla dressing type membrane at-20 deg.C under vacuum degree of 3Pa, and taking out after 28 hr to obtain porous material reinforced rhizoma bletilla dressing.
Wherein the mass ratio of the bletilla striata polysaccharide solution of the connecting layer to the bletilla striata polysaccharide membrane casting solution is 0.3:1, the warp and weft density of the medical oxidized cellulose gauze is 10 threads/in, and the yarn fineness in the gauze is 30 tex.
Wherein, the length of the flat plate mould groove is 50mm, the width is 50mm, and the height is 5 mm.
EXAMPLE III
a preparation of bletilla striata polysaccharide membrane casting solution
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 10%, and placing the solution in a vacuum state with the vacuum degree of 0.5MPa to remove air bubbles to obtain a bletilla striata polysaccharide membrane casting solution for later use.
b preparation of bletilla striata polysaccharide solution of connecting layer
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 15%, and placing the solution in a vacuum state with the vacuum degree of 0.5MPa to remove air bubbles to obtain a bletilla striata polysaccharide solution of a connecting layer.
c preparation of porous material reinforced bletilla striata dressing
Pouring the bletilla striata polysaccharide casting solution prepared in the step a into a groove of a flat plate die, arranging a metal conductor for transferring temperature on the bottom surface of the groove of the flat plate die, wherein the temperature of the metal conductor is-80 ℃, transferring the temperature upwards from the bottom of the die through the casting solution, and when 90% of the total weight of the bletilla striata polysaccharide casting solution in the flat plate die is directionally frozen, pouring the bletilla striata polysaccharide solution of the connecting layer prepared in the step b into the upper part of the bletilla striata polysaccharide casting solution which is not completely frozen, blending the two solutions, horizontally spreading gelatin sponge which is cut into a length, a width and a thickness which are the same as those of the groove of the flat plate die and is 3mm above the blended solution until the gelatin sponge is completely soaked, freezing the bletilla striata polysaccharide casting solution, the bletilla striata polysaccharide solution of the connecting layer and the gelatin sponge into a whole body under the action of the metal conductor to form a porous material reinforced bletilla striata dressing film with the thickness of 8mm, and placing the bletilla dressing film at the temperature of-80 ℃ to be completely soaked, Freeze-drying in an environment with a vacuum degree of 20Pa, and taking out after 72h to obtain the porous material reinforced bletilla striata dressing.
Wherein, the mass ratio of the bletilla striata polysaccharide solution of the connecting layer to the bletilla striata polysaccharide membrane casting solution is 0.5:1, and the gelatin sponge can be obtained by adopting Chinese patent publication No. CN101574539A with the name of invention being a gelatin sponge and a preparation method thereof.
Wherein, the length of the flat plate mould groove is 200mm, the width is 200mm, and the height is 12 mm.
Example four
a preparation of bletilla striata polysaccharide membrane casting solution
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 8%, and placing the solution in a vacuum state with the vacuum degree of 0.3MPa to remove air bubbles to obtain a bletilla striata polysaccharide membrane casting solution for later use.
b preparation of bletilla striata polysaccharide solution of connecting layer
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 14%, and placing the solution in a vacuum state with the vacuum degree of 0.4MPa to remove air bubbles to obtain a bletilla striata polysaccharide solution of a connecting layer.
c preparation of porous material reinforced bletilla striata dressing
Pouring the bletilla striata polysaccharide casting solution prepared in the step a into a groove of a flat plate die, arranging a metal conductor for transferring temperature on the bottom surface of the groove of the flat plate die, wherein the temperature of the metal conductor is-60 ℃, transferring the temperature from the bottom of the die upwards through the casting solution, when 83 percent of the total weight of the bletilla striata polysaccharide casting solution in the flat plate die is directionally frozen, pouring the bletilla striata polysaccharide solution of the connecting layer prepared in the step b into the upper part of the bletilla striata polysaccharide casting solution which is not completely frozen, blending the two solutions, spreading medical oxidized cellulose yarn which is cut into a length, width and thickness which are the same as those of the groove of the flat plate die and is 2mm above the blended solution until the medical oxidized cellulose yarn is completely soaked, and freezing the polysaccharide bletilla striata casting solution, the bletilla striata polysaccharide solution of the connecting layer and the medical oxidized cellulose yarn into a whole under the action of the metal conductor to form a porous material reinforced bletilla striata dressing film with the thickness of 6mm, freeze drying rhizoma bletilla dressing type membrane at-40 deg.C under vacuum degree of 10Pa for 60 hr, and taking out to obtain porous material reinforced rhizoma bletilla dressing.
Wherein the mass ratio of the bletilla striata polysaccharide solution of the connecting layer to the bletilla striata polysaccharide membrane casting solution is 0.4:1, the warp and weft density of the medical oxidized cellulose gauze is 30 threads/in, and the fineness of the threads in the gauze is 60 tex.
Wherein, the length of the flat plate mould groove is 150mm, the width is 100mm, and the height is 10 mm.
EXAMPLE five
a preparation of bletilla striata polysaccharide membrane casting solution
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 6%, and placing the solution in a vacuum state with the vacuum degree of 0.25MPa to remove air bubbles to obtain a bletilla striata polysaccharide membrane casting solution for later use.
b preparation of bletilla striata polysaccharide solution of connecting layer
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 12%, and placing the solution in a vacuum state with the vacuum degree of 0.35MPa to remove air bubbles to obtain a bletilla striata polysaccharide solution of a connecting layer.
c preparation of porous material reinforced bletilla striata dressing
Pouring the bletilla striata polysaccharide casting solution prepared in the step a into a groove of a flat plate die, arranging a metal conductor for transferring temperature on the bottom surface of the groove of the flat plate die, wherein the temperature of the metal conductor is-50 ℃, transferring the temperature from the bottom of the die upwards through the casting solution, when 88% of the total weight of the bletilla striata polysaccharide casting solution in the flat plate die is directionally frozen, pouring the bletilla striata polysaccharide solution of the connecting layer prepared in the step b into the upper part of the bletilla striata polysaccharide casting solution which is not completely frozen, blending the two solutions, spreading medical oxidized cellulose yarn which is cut into a length, width and thickness which are the same as those of the groove of the flat plate die and is 2.6mm above the blended solution until the medical oxidized cellulose yarn is completely soaked, and freezing the bletilla striata polysaccharide casting solution, the bletilla striata polysaccharide solution of the connecting layer and the medical oxidized cellulose yarn into a whole under the action of the metal conductor to form a porous material reinforced bletilla striata, freeze drying rhizoma bletilla dressing type membrane at-30 deg.C under vacuum degree of 12Pa for 39 hr, and taking out to obtain porous material reinforced rhizoma bletilla dressing.
Wherein the mass ratio of the bletilla striata polysaccharide solution of the connecting layer to the bletilla striata polysaccharide membrane casting solution is 0.35:1, the warp and weft density of the medical oxidized cellulose gauze is 20 threads/in, and the yarn fineness in the gauze is 45 tex.
Wherein, the length of the flat plate mould groove is 100mm, the width is 200mm, and the height is 8 mm.
Claims (1)
1. A preparation method of a porous material reinforced bletilla striata dressing is characterized by comprising the following steps:
a preparation of bletilla striata polysaccharide membrane casting solution
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 3-10%, and removing air bubbles under the vacuum condition of 0.1-0.5 MPa of vacuum degree to obtain a bletilla striata polysaccharide membrane casting solution for later use;
b preparation of bletilla striata polysaccharide solution of connecting layer
Dissolving bletilla striata polysaccharide in water to prepare a bletilla striata polysaccharide solution with the mass fraction of 10-15%, and removing air bubbles under the vacuum condition of 0.1-0.5 MPa of vacuum degree to obtain a bletilla striata polysaccharide solution of a connecting layer;
c preparation of porous material reinforced bletilla striata dressing
Pouring the bletilla striata polysaccharide casting solution prepared in the step a into a groove of a flat plate die, arranging a metal conductor for transferring temperature on the bottom surface of the groove of the flat plate die, wherein the temperature of the metal conductor is-20 to-80 ℃, transferring the temperature from the bottom of the die upwards through the casting solution, when the total amount of the bletilla striata polysaccharide casting solution in the flat plate die is directionally frozen by 80 to 90 percent, pouring the bletilla striata polysaccharide solution of the connecting layer prepared in the step b into the upper part of the bletilla striata polysaccharide casting solution which is not completely frozen, melting the two solutions, distributing and flatly paving gelatin sponge or medical oxidized cellulose yarn which is cut into a shape with the same length and width as the groove of the flat plate die and the thickness of 1 to 3mm above the melted solution until the gelatin sponge or the medical oxidized cellulose yarn is completely soaked, and freezing the bletilla striata polysaccharide casting solution, the bletilla striata polysaccharide solution of the connecting layer and the gelatin sponge or the medical oxidized cellulose yarn cloth in the die into a whole under the action of the metal conductor, forming a porous material reinforced bletilla striata dressing type film with the thickness of 3-8 mm, placing the bletilla striata dressing type film in an environment with the temperature of-10 to-80 ℃ and the vacuum degree of 1-20 Pa for freeze drying, and taking out after 24-72 h to obtain the porous material reinforced bletilla striata dressing;
wherein the mass ratio of the bletilla striata polysaccharide solution of the connecting layer to the bletilla striata polysaccharide casting solution is 0.2: 1-0.5: 1, the warp and weft density of the medical oxidized cellulose gauze is 10-50 threads/in, and the fineness of the threads in the gauze is 30-60 tex.
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| CN201810844064.8A CN108904862A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of two-layer compound bletilla striata base dressing |
| CN201810847488.XA CN108939131A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of two-layer compound bletilla striata base dressing |
| CN201810847488X | 2018-07-27 | ||
| CN2018108440648 | 2018-07-27 |
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| CN104189945A (en) * | 2014-09-29 | 2014-12-10 | 福州大学 | Double-layered composite biological dressing and adhesive-free compound technology thereof |
| CN104906620A (en) * | 2015-05-15 | 2015-09-16 | 南阳市汇博生物技术有限公司 | Hydrogel antibacterial gauze dressing and preparation method therefor |
| CN106075536A (en) * | 2016-06-22 | 2016-11-09 | 武汉纺织大学 | A kind of preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) base dressing |
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