CN110215535A - A kind of preparation method of embedded structure formula bletilla striata dressing - Google Patents
A kind of preparation method of embedded structure formula bletilla striata dressing Download PDFInfo
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- CN110215535A CN110215535A CN201910650588.8A CN201910650588A CN110215535A CN 110215535 A CN110215535 A CN 110215535A CN 201910650588 A CN201910650588 A CN 201910650588A CN 110215535 A CN110215535 A CN 110215535A
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- bletilla polysaccharide
- bletilla
- casting solution
- type film
- preparation
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- 241001313857 Bletilla striata Species 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title claims abstract description 53
- 150000004676 glycans Chemical class 0.000 claims abstract description 192
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 190
- 239000005017 polysaccharide Substances 0.000 claims abstract description 190
- 241001313855 Bletilla Species 0.000 claims abstract description 186
- 238000005266 casting Methods 0.000 claims abstract description 132
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 98
- 238000005520 cutting process Methods 0.000 claims abstract description 31
- 238000007710 freezing Methods 0.000 claims abstract description 21
- 230000008014 freezing Effects 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000000243 solution Substances 0.000 claims description 145
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 37
- 229910052751 metal Inorganic materials 0.000 claims description 18
- 239000002184 metal Substances 0.000 claims description 18
- 239000007864 aqueous solution Substances 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 8
- 238000011049 filling Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 238000001727 in vivo Methods 0.000 abstract description 2
- 239000010410 layer Substances 0.000 description 47
- 206010052428 Wound Diseases 0.000 description 12
- 208000027418 Wounds and injury Diseases 0.000 description 12
- 238000000034 method Methods 0.000 description 10
- 239000013078 crystal Substances 0.000 description 5
- 241001523383 Achnatherum Species 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 3
- 230000000025 haemostatic effect Effects 0.000 description 3
- 230000035876 healing Effects 0.000 description 3
- 239000011229 interlayer Substances 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 230000029663 wound healing Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 229940030225 antihemorrhagics Drugs 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002439 hemostatic effect Effects 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/64—Use of materials characterised by their function or physical properties specially adapted to be resorbable inside the body
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A61L2400/00—Materials characterised by their function or physical properties
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Abstract
The present invention relates to a kind of preparation methods of embedded structure formula bletilla striata dressing, belong to biomedicine technical field.Bletilla polysaccharide is dissolved in water and prepares casting solution by preparation method, take part bletilla polysaccharide casting solution, with naoh treatment, embeding layer casting solution is obtained, bletilla polysaccharide casting solution is frozen into bletilla polysaccharide type film, in bletilla polysaccharide type film surface cutting, embeding layer casting solution is poured on bletilla polysaccharide type film cutting face, through cold service, obtains embedded structure formula bletilla striata dressing type film, after freeze-dried, embedded structure formula bletilla striata dressing is obtained.The present invention prepares embedded structure formula bletilla striata dressing using groove engraving and freezing mode, overcomes bletilla striata dressing soluble adhesion gloves, the difficult problem for being detached from docile wound when taking.Gained bletilla striata dressing of the invention can be degraded and absorbed in vivo, good with biocompatible, without side-effects to wound.
Description
Technical field
The present invention relates to a kind of preparation methods of embedded structure formula bletilla striata dressing, belong to biomedicine technical field.
Background technique
In surgery clinical use, the quick-acting haemostatic powder of wound has important influence to the post-operative recovery of patient.It is existing
Bletilla striata dressing is mainly made as porous structure, improves its water imbibition, then enhances its anthemorrhagic performance, so that the composition of bletilla striata dressing
Ingredient-bletilla polysaccharide medicinal efficacy is not fully exerted.Wet environment is provided for wound after bletilla polysaccharide swelling, is accelerated
The healing of wound, and the formation of scar can be inhibited.
China Patent Publication No. is CN106075551A, and publication date is on November 09th, 2016, entitled one kind
The preparation method of intercrossed network type bletilla polysaccharide film, the bletilla polysaccharide preparation solution soluble in water which obtains extraction, benefit
Freeze bletilla polysaccharide solution with two-way ice crystal induced growth techinque, obtaining using freeze-drying has intercrossed network type polysaccharide membrane
Material.The bletilla polysaccharide film of this method preparation can be fully absorbed by human body, and the binding site that network is constructed provides for bletilla polysaccharide film
Good mechanical property on the basis of giving full play to bletilla striata high molecular material haemostatic effect improves the mechanical property of dressing,
Expand its corresponding application field.China Patent Publication No. is CN106075536A, and publication date is on November 09th, 2016, hair
A kind of bright entitled preparation method of bletilla striata base dressing, the product construct fiber-reinforced layer by the way that bletilla polysaccharide is spun into fiber,
Fibre-reinforced bletilla striata base dressing is prepared using the oriented growth and Freeze Drying Technique of ice crystal.The bletilla striata base of this method preparation applies
Expect that internal synusia and long filament formation are mutually perpendicular to cross arrangement structure, good mechanical properties.The preparation method simple process, cost
It is cheap, do not add chemical reagent.But it exists when in use is not easy to take, and surface layer is soluble, is not easy docile wound, reduces it
Haemostatic effect and speed of wound healing.
Existing bletilla striata dressing is led in surgical operation using the reason of speckling with watery blood on the middle gloves that can be worn because of doctor
Dissolution and adhesion are caused, to be unfavorable for dressing in the docile of wound.
Summary of the invention
There are problems for above-mentioned, and the purpose of the present invention is to provide a kind of embedded structure formula bletilla striata dressing preparation methods.
To achieve the above object, technical solution of the invention are as follows:
The preparation of a bletilla polysaccharide casting solution
Bletilla polysaccharide is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 3~10%, is placed in vacuum
Degree is that the vacuum state of 0.1~0.5MPa goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of b sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 1~4%, is stirred evenly,
It is cooled to room temperature.
The preparation of c embeding layer casting solution
The bletilla polysaccharide casting solution for taking part steps a to prepare, sodium hydroxide solution prepared by step b with 0.3~
The speed of 1.0ml/min is added drop-wise in the bletilla polysaccharide casting solution stirred under 200~500r/min speed, after stirring 2~5h,
It is placed in 3~6h in the environment that vacuum degree is 0.05~0.1MPa and removes bubble removing, obtain embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 96:4~99:1.
The preparation of d embedded structure formula bletilla striata dressing
The bletilla polysaccharide casting solution for taking part steps a to prepare pours into the groove of flat plate mold, will be put down using freezing mode
Bletilla polysaccharide casting solution fully charge in board mold forms the bletilla polysaccharide type film of 3~8mm thickness, is milled with sharp inverted T shaped groove
Knife scribes inverted T shaped groove in bletilla polysaccharide type film upper surface with the density of 1~2/cm, and the lower width of rebate of inverted T shaped groove is 1.5~
The width ratio of 3mm, lower notch and upper notch is 2:1~3:1, and the depth of inverted T shaped groove is 1~2mm, and upper notch and lower notch are deep
Degree ratio is 1:1~1:2, and the bletilla polysaccharide type film after cutting is placed horizontally at together with flat plate mold to -10~-80 DEG C of environment
In, the embeding layer casting solution for taking step c to prepare is poured on bletilla polysaccharide type film cutting face, and embeding layer casting solution and bletilla polysaccharide are cast
Volume ratio used in film liquid is 0.2:1~0.8:1, under -10~-80 DEG C of cold services, the bletilla polysaccharide after cutting in mold
Type film freezes to be integrally formed with embeding layer casting solution, forms embedded structure formula bletilla polysaccharide type film, and the embedded structure formula bletilla striata is more
Sugar-type film is placed in that temperature is -20~-60 DEG C, vacuum degree is to be freeze-dried in the environment of 1~20Pa, takes out, that is, obtains after 24~72h
Obtain the bletilla striata dressing of embedded structure formula.
The freezing mode, which refers to, is placed horizontally at -10~-80 DEG C for the flat plate mold for filling bletilla polysaccharide casting solution
Environment in, freeze bletilla polysaccharide casting solution in flat plate mold, form bletilla polysaccharide type film.
The freezing mode refers in flat plate mold groove perpendicular to setting transmitting temperature on a side of bottom surface
Metal transducer, the temperature of metal transducer is -20~-80 DEG C, by the side of flat plate mold setting metal transducer through white
Splendid achnatherum polysaccharide casting solution transmits temperature to side to flat plate mold, and orientation freezes bletilla polysaccharide casting solution in flat plate mold, is formed white
Splendid achnatherum glycan film.
Wherein, a length of 10~200mm of above-mentioned flat plate mold groove, width are 10~200mm, a height of 4~12mm.
Due to using above technical scheme, preparation method of the invention solves what existing bletilla striata dressing faced in
Soluble adhesion, hardly possible are detached from, waste big problem when operation, have achieved the purpose that the efficient facilitated application of bletilla polysaccharide.The present invention
For the concentration of the bletilla polysaccharide casting solution prepared in preparation method between 3%~10%, concentration determines bletilla striata dressing
Pore structure, pore size and mechanical property further influence the effect of bletilla striata dressing in wound healing process.Concentration is lower than
3% gained bletilla striata dressing poor mechanical property, hole configurations is imperfect, easily collapses.When concentration is higher than 10%, the bletilla striata that is prepared
Dressing is partially hard, irritating to wound, uncomfortable, compact structure, is unfavorable for the exchange of inner air and outer air, causes the healing time of wound
Extend.Bletilla polysaccharide solution after being completely dissolved need to be placed in the vacuum state that vacuum degree is 0.1~0.5MPa and go down bubble removing, prevent
The air impact bletilla striata dressing only dissolved into solution be finally freeze-dried after pore structure.
The preparation of embeding layer casting solution, is realized by chemical modification method in preparation method of the present invention, i.e. preparation quality point
The sodium hydrate aqueous solution that number is 1~4%, takes the configured bletilla polysaccharide casting solution in part, under stirring, by hydroxide
Sodium water solution is added dropwise in bletilla polysaccharide casting solution, is removed the acetyl group in bletilla polysaccharide using sodium hydroxide, to obtain
Take embeding layer casting solution.
Bletilla polysaccharide casting solution is frozen by bletilla polysaccharide type film using freezing mode in preparation method of the present invention, wherein
There are two types of freezing modes.First, metal transducer is arranged in flat plate mold groove, under the action of metal transducer, by putting down
Temperature is transmitted to side to flat plate mold through bletilla polysaccharide casting solution in the side that metal transducer is arranged in board mold, and temperature was transmitted
Cheng Zhong, the moisture in bletilla polysaccharide casting solution freeze, grow along temperature direction of transfer, and bletilla polysaccharide molecule is from bletilla polysaccharide casting film
It is precipitated, is fixed in the ice phase gap of ice-crystal growth in liquid, obtain the dressing layer bletilla polysaccharide type film of ice crystal orientation structure；
Second, the flat plate mold for filling bletilla polysaccharide casting solution is placed horizontally in -10~-80 DEG C of environment, freeze flat plate mold
Interior bletilla polysaccharide casting solution, hydrogenesis Cheng Bing at this time, bletilla polysaccharide molecule from being precipitated and be fixed in ice crystal gap in solution,
Ice is formed to connect the bletilla polysaccharide type film of distribution.
The present invention is during preparing embedded structure formula bletilla striata dressing, first by bletilla polysaccharide casting solution freeze forming film,
Then inverted T shaped groove is scribed on its surface, then embeding layer casting solution is poured on bletilla polysaccharide type film cutting face, embeding layer casting solution
Because the gravity of itself and good mobile performance are full of the film cutting of bletilla polysaccharide type, and the shape on bletilla polysaccharide type film cutting face
At certain thickness embeding layer casting solution liquid layer, under -10~-80 DEG C of cold services, in mold after cutting bletilla polysaccharide type film with
Embeding layer casting solution freezes to be integrally formed, and embedded structure formula bletilla polysaccharide type film is formed, by embedded structure formula bletilla polysaccharide type film
It is placed in that temperature is -20~-60 DEG C, vacuum degree is to be freeze-dried in the environment of 1~20Pa, takes out, that is, be embedded in after 24~72h
Layer is insoluble in the embedded structure formula bletilla striata dressing of water, at this point, bletilla striata dressing, which is one, is difficult to isolated entirety, embeding layer casting solution
With volume ratio used in bletilla polysaccharide casting solution be 0.2:1~0.8:1, accounting of the controllable embeding layer dressing in bletilla striata dressing,
Adapt to wound healing process.
Two interlayer of bletilla striata dressing forms insertion closing component structure, can provide for the connection between two layers due to the presence of cutting
Enough mechanical strengths meet the requirement in dressing use process to two interlayer mechanical properties.
The quantity of cutting determines the intensity connected between bilayer, but cutting quantity is more to reduce the strong of bletilla striata dressing
Degree.Therefore, select the density of 1~2/10mm in the upper surface cutting of bletilla polysaccharide film.
The depth of cutting equally affects the compactness connected between the intensity of bletilla striata dressing and two layers.When groove depth is big
When 2mm, the mechanical property of bletilla striata dressing is damaged, and is unable to reach requirement.When groove depth is less than 1mm, two layers
Between chimeric defective tightness, easily slip.Therefore, the groove depth of 1~2mm is selected.
The present invention prepares embedded structure formula bletilla striata dressing using groove engraving and freezing mode, and two interlayer of bletilla striata dressing is chimeric can
It leans on, simple process, overcomes bletilla striata dressing soluble adhesion gloves, the difficult problem for being detached from docile wound when taking.The present invention
The dressing of the gained bletilla striata can be degraded and absorbed in vivo, good with biocompatible, without side-effects to wound, in embeding layer dressing
Low aqueous solubility bletilla polysaccharide molecule solves the problems, such as the readily soluble adhesion difficulty docile that existing bletilla striata dressing faces when in use, so that
Medical effect of bletilla striata dressing is preferably brought into play.
Using embedded structure formula bletilla striata dressing prepared by preparation method of the present invention, the hole configurations of bletilla striata dressing is conducive to pair
The absorption of penetrating fluid keeps the drying of the surface of a wound, and wound is in wet environment, and bletilla polysaccharide is readily soluble, hemostatic bacteriostatic promoting healing
Effect is efficiently played.Embeding layer dressing ensure that doctor takes during surgery by low aqueous solubility bletilla polysaccharide molecular composition
Convenience and when to treatment of wounds dressing and gloves easy disengaging, it is difficult to solve the adhesion that existing bletilla striata dressing faces in
It is detached from, dissolves the problem of waste, achieved the purpose that bletilla polysaccharide efficiently utilizes.
Specific embodiment
Present invention is further described in detail combined with specific embodiments below:
A kind of preparation method of embedded structure formula bletilla striata dressing, the preparation method sequentially include the following steps:
The preparation of a bletilla polysaccharide casting solution
Bletilla polysaccharide is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 3~10%, is placed in vacuum
Degree is that the vacuum state of 0.1~0.5MPa goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of b sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 1~4%, is stirred evenly,
It is cooled to room temperature.
The preparation of c embeding layer casting solution
The bletilla polysaccharide casting solution for taking part steps a to prepare, sodium hydroxide solution prepared by step b with 0.3~
The speed of 1.0ml/min is added drop-wise in the bletilla polysaccharide casting solution stirred under 200~500r/min speed, after stirring 2~5h,
It is placed in 3~6h in the environment that vacuum degree is 0.05~0.1MPa and removes bubble removing, obtain embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 96:4~99:1.
The preparation of d embedded structure formula bletilla striata dressing
The bletilla polysaccharide casting solution for taking part steps a to prepare pours into the groove of flat plate mold, will be put down using freezing mode
Bletilla polysaccharide casting solution fully charge in board mold forms the bletilla polysaccharide type film of 3~8mm thickness, is milled with sharp inverted T shaped groove
Knife scribes inverted T shaped groove in bletilla polysaccharide type film upper surface with the density of 1~2/cm, and the lower width of rebate of inverted T shaped groove is 1.5~
The width ratio of 3mm, lower notch and upper notch is 2:1~3:1, and the depth of inverted T shaped groove is 1~2mm, and upper notch and lower notch are deep
Degree ratio is 1:1~1:2, and the bletilla polysaccharide type film after cutting is placed horizontally at together with flat plate mold to -10~-80 DEG C of environment
In, the embeding layer casting solution for taking step c to prepare is poured on bletilla polysaccharide type film cutting face, and embeding layer casting solution and bletilla polysaccharide are cast
Volume ratio used in film liquid is 0.2:1~0.8:1, under -10~-80 DEG C of cold services, the bletilla polysaccharide after cutting in mold
Type film freezes to be integrally formed with embeding layer casting solution, forms embedded structure formula bletilla polysaccharide type film, and the embedded structure formula bletilla striata is more
Sugar-type film is placed in that temperature is -20~-60 DEG C, vacuum degree is to be freeze-dried in the environment of 1~20Pa, takes out, that is, obtains after 24~72h
Obtain the bletilla striata dressing of embedded structure formula.
The freezing mode, which refers to, is placed horizontally at -10~-80 DEG C for the flat plate mold for filling bletilla polysaccharide casting solution
Environment in, freeze bletilla polysaccharide casting solution in flat plate mold, form bletilla polysaccharide type film.
The freezing mode refers in flat plate mold groove perpendicular to setting transmitting temperature on a side of bottom surface
Metal transducer, the temperature of metal transducer is -20~-80 DEG C, by the side of flat plate mold setting metal transducer through white
Splendid achnatherum polysaccharide casting solution transmits temperature to side to flat plate mold, and orientation freezes bletilla polysaccharide casting solution in flat plate mold, is formed white
Splendid achnatherum glycan film.
Wherein, a length of 10~200mm of above-mentioned flat plate mold groove, width are 10~200mm, a height of 4~12mm.
Specific embodiment
It is prepared as described above method
Embodiment one
The preparation of a bletilla polysaccharide casting solution
Bletilla polysaccharide is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 3%, being placed in vacuum degree is
The vacuum state of 0.1MPa goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of b sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 1%, is stirred evenly, it is cooling
To room temperature.
The preparation of c embeding layer casting solution
The bletilla polysaccharide casting solution for taking part steps a to prepare, sodium hydroxide solution prepared by step b is with 0.3ml/min
Speed be added drop-wise in the bletilla polysaccharide casting solution stirred under 200r/min speed, stir 2h after, being placed in vacuum degree is
3h removes bubble removing in the environment of 0.05MPa, obtains embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 96:4.
The preparation of d embedded structure formula bletilla striata dressing
The bletilla polysaccharide casting solution for taking part steps a to prepare pours into the groove of flat plate mold, will be put down using freezing mode
Bletilla polysaccharide casting solution fully charge in board mold forms the bletilla polysaccharide type film of 3mm thickness, with sharp inverted T shaped slotting cutter
Inverted T shaped groove is scribed with the density of 1/cm in bletilla polysaccharide type film upper surface, the lower width of rebate of inverted T shaped groove is 3mm, lower notch
Width ratio with upper notch is 3:1, and the depth of inverted T shaped groove is 2mm, and upper notch and lower depth of rebate ratio are 1:2, by cutting
Bletilla polysaccharide type film afterwards is placed horizontally in -10 DEG C of environment together with flat plate mold, the embeding layer casting solution for taking step c to prepare
It is poured on bletilla polysaccharide type film cutting face, volume ratio used in embeding layer casting solution and bletilla polysaccharide casting solution is 0.8:1, -10
Under DEG C cold service, until bletilla polysaccharide type film freezes to be integrally formed with embeding layer casting solution after cutting in mold, is formed and be embedded in
Embedded structure formula bletilla polysaccharide type film is placed in the environment that temperature is -20 DEG C, vacuum degree is 1Pa by structural formula bletilla polysaccharide type film
Middle freeze-drying, takes out afterwards for 24 hours, i.e. acquisition embedded structure formula bletilla striata dressing.
The freezing mode, which refers to, is placed horizontally at the flat plate mold for filling bletilla polysaccharide casting solution -10 DEG C of environment
In, freeze bletilla polysaccharide casting solution in flat plate mold, forms bletilla polysaccharide type film.
Wherein, a length of 100mm of above-mentioned flat plate mold groove, width 100mm, a height of 6mm.
Embodiment two
The preparation of a bletilla polysaccharide casting solution
Bletilla polysaccharide is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 10%, being placed in vacuum degree is
The vacuum state of 0.5MPa goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of b sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 4%, is stirred evenly, it is cooling
To room temperature.
The preparation of c embeding layer casting solution
The bletilla polysaccharide casting solution for taking part steps a to prepare, sodium hydroxide solution prepared by step b is with 1.0ml/min
Speed be added drop-wise in the bletilla polysaccharide casting solution stirred under 500r/min speed, stir 5h after, being placed in vacuum degree is
6h removes bubble removing in the environment of 0.1MPa, obtains embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 99:1.
The preparation of d embedded structure formula bletilla striata dressing
The bletilla polysaccharide casting solution for taking part steps a to prepare pours into the groove of flat plate mold, will be put down using freezing mode
Bletilla polysaccharide casting solution fully charge in board mold forms the bletilla polysaccharide type film of 8mm thickness, with sharp inverted T shaped slotting cutter
Inverted T shaped groove is scribed with the density of 2/cm in bletilla polysaccharide type film upper surface, the lower width of rebate of inverted T shaped groove is 1.5mm, lower slot
The width ratio of mouth and upper notch is 2:1, and the depth of inverted T shaped groove is 1mm, and upper notch and lower depth of rebate ratio are 1:1, will be carved
Bletilla polysaccharide type film after slot is placed horizontally in -80 DEG C of environment together with flat plate mold, the embeding layer casting film for taking step c to prepare
Liquid is poured on bletilla polysaccharide type film cutting face, and volume ratio used in embeding layer casting solution and bletilla polysaccharide casting solution is 0.2:1 ,-
Under 80 DEG C of cold services, until bletilla polysaccharide type film freezes to be integrally formed with embeding layer casting solution after cutting in mold, formed embedding
Enter structural formula bletilla polysaccharide type film, embedded structure formula bletilla polysaccharide type film is placed in the ring that temperature is -60 DEG C, vacuum degree is 20Pa
It is freeze-dried in border, is taken out after 72h, i.e. acquisition embedded structure formula bletilla striata dressing.
The freezing mode, which refers to, is placed horizontally at the flat plate mold for filling bletilla polysaccharide casting solution -80 DEG C of environment
In, freeze bletilla polysaccharide casting solution in flat plate mold, forms bletilla polysaccharide type film.
Wherein, a length of 200mm of above-mentioned flat plate mold groove, width 100mm, a height of 12mm.
Embodiment three
The preparation of a bletilla polysaccharide casting solution
Bletilla polysaccharide is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 5%, being placed in vacuum degree is
The vacuum state of 0.3MPa goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of b sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 3.5%, is stirred evenly, it is cold
But to room temperature.
The preparation of c embeding layer casting solution
The bletilla polysaccharide casting solution for taking part steps a to prepare, sodium hydroxide solution prepared by step b is with 0.5ml/min
Speed be added drop-wise in the bletilla polysaccharide casting solution stirred under 260r/min speed, stir 3h after, being placed in vacuum degree is
4h removes bubble removing in the environment of 0.06MPa, obtains embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 97:3.
The preparation of d embedded structure formula bletilla striata dressing
The bletilla polysaccharide casting solution for taking part steps a to prepare pours into the groove of flat plate mold, will be put down using freezing mode
Bletilla polysaccharide casting solution fully charge in board mold forms the bletilla polysaccharide type film of 5mm thickness, with sharp inverted T shaped slotting cutter
Inverted T shaped groove is scribed with the density of 1.5/cm in bletilla polysaccharide type film upper surface, the lower width of rebate of inverted T shaped groove is 2.8mm, under
The width ratio of notch and upper notch is 3:1, and the depth of inverted T shaped groove is 1.8mm, and upper notch and lower depth of rebate ratio are 1:
1.5, the bletilla polysaccharide type film after cutting is placed horizontally in -60 DEG C of environment together with flat plate mold, takes step c to prepare embedding
Enter a layer casting solution to be poured on bletilla polysaccharide type film cutting face, embeding layer casting solution is with volume ratio used in bletilla polysaccharide casting solution
0.6:1, under -60 DEG C of cold services, until bletilla polysaccharide type film and embeding layer casting solution freeze to become one after cutting in mold
Body forms embedded structure formula bletilla polysaccharide type film, and it is -50 DEG C, vacuum degree that embedded structure formula bletilla polysaccharide type film, which is placed in temperature,
To be freeze-dried in the environment of 10Pa, taken out after 48h, i.e. acquisition embedded structure formula bletilla striata dressing.
The freezing mode refers in flat plate mold groove perpendicular to setting transmitting temperature on a side of bottom surface
Metal transducer, the temperature of metal transducer is -20 DEG C, by the side of flat plate mold setting metal transducer through bletilla polysaccharide
Casting solution transmits temperature to side to flat plate mold, and orientation freezes bletilla polysaccharide casting solution in flat plate mold, forms bletilla polysaccharide
Type film.
Wherein, a length of 180mm of above-mentioned flat plate mold groove, width 60mm, a height of 8mm.
Example IV
The preparation of a bletilla polysaccharide casting solution
Bletilla polysaccharide is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 5%, being placed in vacuum degree is
The vacuum state of 0.25MPa goes down bubble removing, obtains bletilla polysaccharide casting solution, spare.
The preparation of b sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 2.6%, is stirred evenly, it is cold
But to room temperature.
The preparation of c embeding layer casting solution
The bletilla polysaccharide casting solution for taking part steps a to prepare, sodium hydroxide solution prepared by step b is with 0.8ml/min
Speed be added drop-wise in the bletilla polysaccharide casting solution stirred under 290r/min speed, stir 4.3h after, being placed in vacuum degree is
5h removes bubble removing in the environment of 0.07MPa, obtains embeding layer casting solution.
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 98:2.
The preparation of d embedded structure formula bletilla striata dressing
The bletilla polysaccharide casting solution for taking part steps a to prepare pours into the groove of flat plate mold, will be put down using freezing mode
Bletilla polysaccharide casting solution fully charge in board mold forms the bletilla polysaccharide type film of 6mm thickness, with sharp inverted T shaped slotting cutter
Inverted T shaped groove is scribed with the density of 2/cm in bletilla polysaccharide type film upper surface, the lower width of rebate of inverted T shaped groove is 1.5mm, lower slot
The width ratio of mouth and upper notch is 2.5:1, and the depth of inverted T shaped groove is 1.6mm, and upper notch and lower depth of rebate ratio are 1:
1.8, the bletilla polysaccharide type film after cutting is placed horizontally in -60 DEG C of environment together with flat plate mold, takes step c to prepare embedding
Enter a layer casting solution to be poured on bletilla polysaccharide type film cutting face, embeding layer casting solution is with volume ratio used in bletilla polysaccharide casting solution
0.5:1, under -60 DEG C of cold services, until bletilla polysaccharide type film and embeding layer casting solution freeze to become one after cutting in mold
Body forms embedded structure formula bletilla polysaccharide type film, and it is -50 DEG C, vacuum degree that embedded structure formula bletilla polysaccharide type film, which is placed in temperature,
To be freeze-dried in the environment of 18Pa, taken out after 56h, i.e. acquisition embedded structure formula bletilla striata dressing.
The freezing mode refers in flat plate mold groove perpendicular to setting transmitting temperature on a side of bottom surface
Metal transducer, the temperature of metal transducer is -60 DEG C, by the side of flat plate mold setting metal transducer through bletilla polysaccharide
Casting solution transmits temperature to side to flat plate mold, and orientation freezes bletilla polysaccharide casting solution in flat plate mold, forms bletilla polysaccharide
Type film.
Wherein, a length of 100mm of above-mentioned flat plate mold groove, width 180mm, a height of 10mm.
Claims (3)
1. a kind of preparation method of embedded structure formula bletilla striata dressing, which is characterized in that preparation method sequentially includes the following steps:
The preparation of a bletilla polysaccharide casting solution
Bletilla polysaccharide is dissolved in water and makes the bletilla polysaccharide solution that mass fraction is 3~10%, being placed in vacuum degree is
The vacuum state of 0.1~0.5MPa goes down bubble removing, obtains bletilla polysaccharide casting solution, spare；
The preparation of b sodium hydrate aqueous solution
Sodium hydroxide is dissolved in water and makes the sodium hydrate aqueous solution that mass fraction is 1~4%, is stirred evenly, it is cooling
To room temperature；
The preparation of c embeding layer casting solution
The bletilla polysaccharide casting solution for taking part steps a to prepare, sodium hydroxide solution prepared by step b is with 0.3~1.0ml/min
Speed be added drop-wise in the bletilla polysaccharide casting solution stirred under 200~500r/min speed, stir 2~5h after, be placed in vacuum
Degree is that 3~6h removes bubble removing in the environment of 0.05~0.1MPa, obtains embeding layer casting solution；
Wherein, the mass ratio of bletilla polysaccharide casting solution and sodium hydroxide is 96:4~99:1；
The preparation of d embedded structure formula bletilla striata dressing
The bletilla polysaccharide casting solution for taking part steps a to prepare pours into the groove of flat plate mold, using freezing mode by plate mould
Bletilla polysaccharide casting solution fully charge in tool forms the bletilla polysaccharide type film of 3~8mm thickness, is existed with sharp inverted T shaped slotting cutter
Inverted T shaped groove is scribed with the density of 1~2/cm in bletilla polysaccharide type film upper surface, and the lower width of rebate of inverted T shaped groove is 1.5~3mm,
The width ratio of lower notch and upper notch is 2:1~3:1, and the depth of inverted T shaped groove is 1~2mm, upper notch and lower depth of rebate ratio
Example is 1:1~1:2, and the bletilla polysaccharide type film after cutting is placed horizontally in -10~-80 DEG C of environment together with flat plate mold,
The embeding layer casting solution for taking step c to prepare is poured on bletilla polysaccharide type film cutting face, embeding layer casting solution and bletilla polysaccharide casting film
Volume ratio used in liquid is 0.2:1~0.8:1, under -10~-80 DEG C of cold services, the bletilla polysaccharide type after cutting in mold
Film freezes to be integrally formed with embeding layer casting solution, embedded structure formula bletilla polysaccharide type film is formed, by embedded structure formula bletilla polysaccharide
Type film is placed in that temperature is -20~-60 DEG C, vacuum degree is to be freeze-dried in the environment of 1~20Pa, takes out, that is, obtains after 24~72h
Embedded structure formula bletilla striata dressing.
2. a kind of preparation method of embedded structure formula bletilla striata dressing as described in claim 1, which is characterized in that the freezing
Mode refers in the environment that the flat plate mold for filling bletilla polysaccharide casting solution is placed horizontally to -10~-80 DEG C, freezes plate mould
Bletilla polysaccharide casting solution in having, forms bletilla polysaccharide type film.
3. a kind of preparation method of embedded structure formula bletilla striata dressing as described in claim 1, which is characterized in that the freezing
Mode refers to that, perpendicular to the metal transducer of setting transmitting temperature on a side of bottom surface in flat plate mold groove, metal passes
The temperature for leading device is -20~-80 DEG C, from the side of flat plate mold setting metal transducer through bletilla polysaccharide casting solution to plate mould
Tool transmits temperature to side, and orientation freezes bletilla polysaccharide casting solution in flat plate mold, forms bletilla polysaccharide type film.
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| CN2018108513831 | 2018-07-27 | ||
| CN201810851657.7A CN108904867A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of the embedded double-deck bletilla striata dressing |
| CN2018108516577 | 2018-07-27 | ||
| CN201810851383.1A CN108904865A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method of the embedded double-deck bletilla striata dressing |
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| CN101249274A (en) * | 2008-04-01 | 2008-08-27 | 南京大学 | Preparation of bletilla striata polyose water gelatin of promoting wound healing and uses thereof |
| CN105153458A (en) * | 2015-07-24 | 2015-12-16 | 武汉纺织大学 | Preparation method for low-water-solubility bletilla striata polysaccharide porous membrane |
| CN106075551A (en) * | 2016-06-22 | 2016-11-09 | 武汉纺织大学 | A kind of preparation method of intercrossed network type Pseudobulbus Bletillae polysaccharose film |
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Effective date of registration: 20240222 Address after: Room 1018-15, Building 23, Times Square, Suzhou Industrial Park, Suzhou Area, China (Jiangsu) Pilot Free Trade Zone, Suzhou City, Jiangsu Province, 215000 Patentee after: Suzhou 30 billion Technology Co.,Ltd. Country or region after: China Address before: 430200 1 Sunshine Avenue, Jiangxia District, Wuhan, Hubei. Patentee before: Wuhan Textile University Country or region before: China |