CN107126576A - A kind of composite regenerated cellulosic wound dressings of kapok and preparation method thereof - Google Patents
A kind of composite regenerated cellulosic wound dressings of kapok and preparation method thereof Download PDFInfo
- Publication number
- CN107126576A CN107126576A CN201710264339.6A CN201710264339A CN107126576A CN 107126576 A CN107126576 A CN 107126576A CN 201710264339 A CN201710264339 A CN 201710264339A CN 107126576 A CN107126576 A CN 107126576A
- Authority
- CN
- China
- Prior art keywords
- parts
- solution
- kapok
- wound dressings
- regenerated cellulosic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
- A61L15/325—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
- A61L2300/604—Biodegradation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Hematology (AREA)
- Public Health (AREA)
- Materials Engineering (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Dispersion Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Materials For Medical Uses (AREA)
Abstract
A kind of composite regenerated cellulosic wound dressings of kapok and preparation method thereof, belong to composite biological material field, include the raw material of following parts by weight：60 70 parts of cotton linter, 20 30 parts of bombax cotton, 10 20 parts of fibrosis collagen solution, 5 10 parts of fibroin solutions；The composite regenerated cellulosic wound dressings of kapok produced by the present invention can be used for the healing of the various surface of a wound and with excellent tissue repairing ability.
Description
Technical field
The invention belongs to composite biological material field, more particularly to a kind of composite regenerated cellulosic wound dressings of kapok and its
Preparation method.
Background technology
, it is necessary to which interim substitute using skin regeneration material as damaged skin protects wound after skin injury,
Wound infection and severe dehydration are prevented, the environment of relative moistening, promotion organization regeneration and wound healing are provided for wound healing.
In numerous skin regeneration materials, autologous skin is a kind of optimal repair materials, because it will not produce immune response and containing various
Promote the histocyte and active growth factor of healing.Due to the limited source of autologous skin, the skin of small range can only be used as
Skin is damaged, so when large area skin wounds, it is necessary to by other skin regeneration materials, such as medical wound dressing, skin branch
Frame material, artificial skin etc. realize injury repair as the interim substitute of injured skin, promote cambium regeneration and
The quick healing of wound.
With the development of organizational engineering, skin regeneration material experienced from traditional dressing to new pattern compress again to skin group
The development course of engineering scaffold material is knitted, the skin tissue engineering being made using natural activity macromolecule or artificial synthesized macromolecule
Timbering material is clinically widely used.Although various wound dressings with its it is different the characteristics of be applied to it is clinical not
The same surface of a wound, also disclosure satisfy that the requirement of various wound healings, prior art without a kind of preferable dressing up to now
In the tissue repairing ability of wound dressing need further lifting.
The content of the invention
In order to solve problems of the prior art, it can be used for various wound healings the invention provides one kind
, composite regenerated cellulosic wound dressings of kapok with excellent tissue repairing ability.
The present invention uses following technical scheme：
A kind of composite regenerated cellulosic wound dressings of kapok, include the raw material of following parts by weight：60-70 parts of cotton linter,
20-30 parts of bombax cotton, 10-20 parts of fibrosis collagen solution, 5-10 parts of fibroin solutions；
It is preferred that, described fibrosis collagen solution is prepared using following methods：3mg/mL collagen is molten
Liquid and 10 × PBS are with 4:1 volume ratio is fully mixed at 4 DEG C, and pH to 8 is adjusted with 0.1M NaOH, is added into solution
NaH2PO4And NaCl, and make the final concentration of 2mg/mL, NaH of collagen solution2PO4Final concentration of 10mM, NaCl end
Concentration is 100mM, then by 35 DEG C of water-bath 24h of solution transposition, after reaction terminates, and is centrifuged off supernatant, and will precipitation
It is dissolved in distilled water, desalination of dialysing produces the collagen solution of fibrosis.
It is preferred that, described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, added
4g sodium carbonate and 5g silkworm raw silks, boil after 30min, are cleaned up and dried with deionized water, then with 500ml, 9.3mol/L
The dissolving of LiBr solution, solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation
20min, is repeated twice, and obtains fibroin solutions.
It is preferred that, a kind of described composite regenerated cellulosic wound dressings of kapok, in addition to 3-5 parts of Chinese medical extract.
It is preferred that, described Chinese medical extract is Shorthorned Epimedium P.E, Rhizoma Drynariae extract, Rhizoma Chuanxiong extract, Morinda officinalis
One or more kinds of combinations in extract, Eupolyphaga seu steleophaga extract, Salvia root P.E.
It is preferred that, described Chinese medical extract is prepared using following methods：Chinese medicine 20-25g is weighed, coarse powder is ground into, plus
Enter the distilled water immersion 1-2h of 10-15 times of its weight, refluxing extraction 1-1.5h, suction filtration obtains filtrate 1；8-10 times is added into filter residue
The distilled water of filter residue weight, refluxing extraction 1-1.5h, suction filtration obtains filtrate 2；The steaming of 8-10 times of filter residue weight is added into filter residue again
Distilled water, refluxing extraction 1-1.5h, suction filtration obtains filtrate 3；Filtrate 1, filtrate 2, filtrate 3 are merged, are 40-50 DEG C, vacuum in temperature
Spend to be concentrated under reduced pressure into 4-6 times of Chinese medicine weight under the conditions of 0.05-0.08Pa, with three layers of filtered through gauze, produce Chinese medicine extract.
A kind of preparation method of the composite regenerated cellulosic wound dressings of kapok, comprises the following steps：
Step one：Cotton linter, bombax cotton are weighed, is dissolved in lithium hydroxide/aqueous solution of urea, is configured to quality
The cellulose solution of concentration 4%, described lithium hydroxide/aqueous solution of urea uses lithium hydroxide 8.76g, urea 12g and water
79.24g is formulated, and is stirred, filters, is stood 24h, electrostatic spinning obtains tunica fibrosa under the conditions of 20kV；
Step 2：Fibrosis collagen solution, fibroin solutions are well mixed, tunica fibrosa surface are applied to, i.e.,
Obtain dressing.
A kind of preparation method of the composite regenerated cellulosic wound dressings of kapok, comprises the following steps：
Step one：Cotton linter, bombax cotton are weighed, is dissolved in lithium hydroxide/aqueous solution of urea, is configured to quality
The cellulose solution of concentration 4%, described lithium hydroxide/aqueous solution of urea uses lithium hydroxide 8.76g, urea 12g and water
79.24g is formulated, and is stirred, filters, is stood 24h, electrostatic spinning obtains tunica fibrosa under the conditions of 20kV；
Step 2：Fibrosis collagen solution, fibroin solutions and Chinese medical extract are well mixed, fibre is applied to
Film surface is tieed up, dressing is produced.
The beneficial effects of the present invention are：
1) cellulose, with good biocompatibility and bioactivity, can act on the surface of a wound as a kind of biomaterial
The multiple links repaired, promote the quick healing of the surface of a wound.It is the wound dressing soft and transparent that is made using cellulose as raw material, comfortable；
There is good permeability, moistening, slightly sour repairing environment can be provided for the surface of a wound；Surface of a wound oxygen tension can be adjusted, promotes capillary
Formed, so as to accelerate the healing of wound.
2) main component of collagen inherently extracellular matrix, collagen can be mutual with cell or surrounding substrate
Effect, shows good harmony；In collagen molecules and intermolecular covalent cross-linking to impart collagen more high-strength
The mechanical performance of degree；Collagen can and platelet adhesion, cohesion form thrombus and prevent blood flow, so as to play the effect of hemostasis
Really；The network skeleton structure of collagen plays grappling, supporting function to cell, can promote growth, propagation and the migration of cell.
3) fibroin biocompatibility is good, and degradation speed is slow and catabolite has low immunogenicity to tissue,
Nutritious and repair ability is organized to skin, periodontal etc., when cultivating cell in vivo or in vitro, the fibroin albumen energy of loose structure
Sticking, sprawl and breeding for cell is adapted to, the repair and reconstruction to tissue are extremely advantageous, are a kind of biological materials of excellent performance
Material.
4) the composite regenerated cellulosic wound dressings of kapok produced by the present invention can be used for the various surface of a wound healing and
With excellent tissue repairing ability.
Embodiment
Embodiment 1
A kind of composite regenerated cellulosic wound dressings of kapok, include the raw material of following parts by weight：60 parts of cotton linter, kapok
20 parts of fiber, 10 parts of fibroin solutions, 5 parts of fibrosis collagen solution.
Described fibrosis collagen solution is prepared using following methods：By 3mg/mL collagen solution and 10 ×
PBS is with 4:1 volume ratio is fully mixed at 4 DEG C, adjusts pH to 8 with 0.1M NaOH, NaH is added into solution2PO4And
NaCl, and make the final concentration of 2mg/mL, NaH of collagen solution2PO4Final concentration of 10mM, NaCl's is final concentration of
100mM, then by 35 DEG C of water-bath 24h of solution transposition, after reaction terminates, is centrifuged off supernatant, and precipitation is dissolved in into steaming
In distilled water, desalination of dialysing produces the collagen solution of fibrosis.
Described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, 4g carbonic acid is added
Sodium and 5g silkworm raw silks, boil after 30min, are cleaned up and dried with deionized water, then with 500ml, 9.3mol/L LiBr
Solution is dissolved, and solution is loaded into bag filter until completely dissolved, and deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated
Twice, fibroin solutions are obtained.
A kind of preparation method of the composite regenerated cellulosic wound dressings of kapok, comprises the following steps：
Step one：Cotton linter, bombax cotton are weighed, is dissolved in lithium hydroxide/aqueous solution of urea, is configured to quality
The cellulose solution of concentration 4%, described lithium hydroxide/aqueous solution of urea uses lithium hydroxide 8.76g, urea 12g and water
79.24g is formulated, and is stirred, filters, is stood 24h, electrostatic spinning obtains tunica fibrosa under the conditions of 20kV；
Step 2：Fibrosis collagen solution, fibroin solutions are well mixed, tunica fibrosa surface are applied to, i.e.,
Obtain dressing.
Embodiment 2
A kind of composite regenerated cellulosic wound dressings of kapok, include the raw material of following parts by weight：70 parts of cotton linter, kapok
30 parts of fiber, 20 parts of fibrosis collagen solution, 10 parts of fibroin solutions, 3 parts of Chinese medical extract.
Described fibrosis collagen solution is prepared using following methods：By 3mg/mL collagen solution and 10 ×
PBS is with 4:1 volume ratio is fully mixed at 4 DEG C, adjusts pH to 8 with 0.1M NaOH, NaH is added into solution2PO4And
NaCl, and make the final concentration of 2mg/mL, NaH of collagen solution2PO4Final concentration of 10mM, NaCl's is final concentration of
100mM, then by 35 DEG C of water-bath 24h of solution transposition, after reaction terminates, is centrifuged off supernatant, and precipitation is dissolved in into steaming
In distilled water, desalination of dialysing produces the collagen solution of fibrosis.
Described fibroin solutions are prepared using following methods：2L deionized waters are heated to boiling, 4g carbonic acid is added
Sodium and 5g silkworm raw silks, boil after 30min, are cleaned up and dried with deionized water, then with 500ml, 9.3mol/L LiBr
Solution is dissolved, and solution is loaded into bag filter until completely dissolved, and deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated
Twice, fibroin solutions are obtained.
Described Chinese medical extract be Shorthorned Epimedium P.E, Rhizoma Drynariae extract, Rhizoma Chuanxiong extract, Radix Morindae Officinalis extract,
Eupolyphaga seu steleophaga extract, Salvia root P.E are by weight 1:1:1:1:1:1 mixture.
Described Chinese medical extract is prepared using following methods：Chinese medicine 20-25g is weighed, coarse powder is ground into, its weight is added
10-15 times of distilled water immersion 1-2h, refluxing extraction 1-1.5h, suction filtration obtains filtrate 1；8-10 times of filter residue weight is added into filter residue
Distilled water, refluxing extraction 1-1.5h, suction filtration obtains filtrate 2；The distilled water of 8-10 times of filter residue weight, backflow are added into filter residue again
1-1.5h is extracted, suction filtration obtains filtrate 3；Filtrate 1, filtrate 2, filtrate 3 are merged, temperature be 40-50 DEG C, vacuum be 0.05-
4-6 times of Chinese medicine weight is concentrated under reduced pressure under the conditions of 0.08Pa, with three layers of filtered through gauze, Chinese medicine extract is produced.
A kind of preparation method of the composite regenerated cellulosic wound dressings of kapok, comprises the following steps：
Step one：Cotton linter, bombax cotton are weighed, is dissolved in lithium hydroxide/aqueous solution of urea, is configured to quality
The cellulose solution of concentration 4%, described lithium hydroxide/aqueous solution of urea uses lithium hydroxide 8.76g, urea 12g and water
79.24g is formulated, and is stirred, filters, is stood 24h, electrostatic spinning obtains tunica fibrosa under the conditions of 20kV；
Step 2：Fibrosis collagen solution, fibroin solutions and Chinese medical extract are well mixed, fibre is applied to
Film surface is tieed up, dressing is produced.
12 small white mouses are selected, 3 groups is randomly divided into, every group 4,12 × 2cm is produced on the backbone of every white mouse
Full thickness dermal wound model, blank control group and two experimental groups (embodiment 1, embodiment 2) are divided into again.In experiment
Observe the general condition and healing time of wound healing daily afterwards；Simultaneously wound tissue, formalin solution are taken at surface of a wound center
It is fixed, FFPE, soft tissue slices, row HE dyeing, observes blank control group under the microscope and experimental group skin holostrome lacks
The healing state in damage face.7th, 14,21,28, the surface of a wound photograph respectively to experimental group and blank control group in 35 days adopt figure, use tricks
Calculation machine image analysis software calculates the wound healing area of each group, determines the wound healing percentage of each group, is cured by the formula surface of a wound
Conjunction rate (%)=[(surface of a wound original area-surface of a wound do not heal area)/surface of a wound original area] × 100%, as a result see the table below 1.
The each group Wound healing rate of table 1 compares
As a result：Understood in the healing time and Wound healing rate of contrast blank control group and the experimental group of application dressing：Wound
Face ordinary circumstance experimental group is significantly better than blank control group, and the dressing used in experimental group has good promoting healing to defect of skin
Effect.
Claims (9)
1. a kind of composite regenerated cellulosic wound dressings of kapok, it is characterised in that include the raw material of following parts by weight：Cotton linter
60-70 parts, 20-30 parts of bombax cotton, 10-20 parts of fibrosis collagen solution, 5-10 parts of fibroin solutions；
Described fibrosis collagen solution is prepared using following methods：By 3mg/mL collagen solution and 10 × PBS
With 4:1 volume ratio is fully mixed at 4 DEG C, adjusts pH to 8 with 0.1M NaOH, NaH is added into solution2PO4And NaCl, and
Make the final concentration of 2mg/mL, NaH of collagen solution2PO4Final concentration of 10mM, NaCl final concentration of 100mM, then
By 35 DEG C of water-bath 24h of solution transposition, after reaction terminates, supernatant is centrifuged off, and precipitation is dissolved in distilled water, is dialysed
Desalination, produces the collagen solution of fibrosis.
2. a kind of composite regenerated cellulosic wound dressings of kapok according to claim 1, it is characterised in that described silk
Protein solution is prepared using following methods：2L deionized waters are heated to boiling, 4g sodium carbonate and 5g silkworm raw silks is added, boils
After 30min, cleaned up and dried with deionized water, then dissolved with 500ml, 9.3mol/L LiBr solution, wait to be completely dissolved
Solution is loaded into bag filter afterwards, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice, obtains fibroin molten
Liquid.
3. the composite regenerated cellulosic wound dressings of a kind of kapok according to claim 1, it is characterised in that including following heavy
Measure the raw material of number：60 parts of cotton linter, 20 parts of bombax cotton, 10 parts of fibroin solutions, 5 parts of fibrosis collagen solution.
4. the composite regenerated cellulosic wound dressings of a kind of kapok according to claim 1, it is characterised in that including following heavy
Measure the raw material of number：70 parts of cotton linter, 30 parts of bombax cotton, 20 parts of fibrosis collagen solution, 10 parts of fibroin solutions.
5. the composite regenerated cellulosic wound dressings of a kind of kapok according to claim 1, it is characterised in that：Also include Chinese medicine
3-5 parts of extract.
6. the composite regenerated cellulosic wound dressings of a kind of kapok according to claim 5, it is characterised in that：Described Chinese medicine
Extract is Shorthorned Epimedium P.E, Rhizoma Drynariae extract, Rhizoma Chuanxiong extract, Radix Morindae Officinalis extract, Eupolyphaga seu steleophaga extract, the red sage root are carried
Take one or more kinds of combinations in thing.
7. a kind of composite regenerated cellulosic wound dressings of kapok according to claim 5, it is characterised in that described Chinese medicine
Extract is prepared using following methods：Chinese medicine 20-25g is weighed, coarse powder is ground into, the distillation water logging of 10-15 times of its weight is added
1-2h is steeped, refluxing extraction 1-1.5h, suction filtration obtains filtrate 1；The distilled water of 8-10 times of filter residue weight, refluxing extraction are added into filter residue
1-1.5h, suction filtration obtains filtrate 2；The distilled water of 8-10 times of filter residue weight is added into filter residue again, refluxing extraction 1-1.5h, suction filtration is obtained
Filtrate 3；Filtrate 1, filtrate 2, filtrate 3 are merged, temperature be 40-50 DEG C, vacuum be depressurize under the conditions of 0.05-0.08Pa it is dense
4-6 times of Chinese medicine weight is reduced to, with three layers of filtered through gauze, Chinese medicine extract is produced.
8. a kind of preparation method of composite regenerated cellulosic wound dressings of kapok as described in claim any one of 1-4, it is special
Levy and be, comprise the following steps：
Step one：Cotton linter, bombax cotton are weighed, is dissolved in lithium hydroxide/aqueous solution of urea, is configured to mass concentration
4% cellulose solution, described lithium hydroxide/aqueous solution of urea uses lithium hydroxide 8.76g, urea 12g and water 79.24g
It is formulated, stirs, filters, stands 24h, electrostatic spinning obtains tunica fibrosa under the conditions of 20kV；
Step 2：Fibrosis collagen solution, fibroin solutions are well mixed, tunica fibrosa surface is applied to, produced deposited
Material.
9. a kind of preparation method of composite regenerated cellulosic wound dressings of kapok as described in claim any one of 5-7, it is special
Levy and be, comprise the following steps：
Step one：Cotton linter, bombax cotton are weighed, is dissolved in lithium hydroxide/aqueous solution of urea, is configured to mass concentration
4% cellulose solution, described lithium hydroxide/aqueous solution of urea uses lithium hydroxide 8.76g, urea 12g and water 79.24g
It is formulated, stirs, filters, stands 24h, electrostatic spinning obtains tunica fibrosa under the conditions of 20kV；
Step 2：Fibrosis collagen solution, fibroin solutions and Chinese medical extract are well mixed, tunica fibrosa is applied to
Surface, produces dressing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710264339.6A CN107126576A (en) | 2017-04-21 | 2017-04-21 | A kind of composite regenerated cellulosic wound dressings of kapok and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710264339.6A CN107126576A (en) | 2017-04-21 | 2017-04-21 | A kind of composite regenerated cellulosic wound dressings of kapok and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107126576A true CN107126576A (en) | 2017-09-05 |
Family
ID=59716781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710264339.6A Pending CN107126576A (en) | 2017-04-21 | 2017-04-21 | A kind of composite regenerated cellulosic wound dressings of kapok and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107126576A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112675356A (en) * | 2020-12-23 | 2021-04-20 | 湖南工业大学 | Long-term-preservation hemostatic composition, preparation method thereof and preparation method of long-term-preservation antibacterial hemostatic mixture |
| CN113975448A (en) * | 2021-11-26 | 2022-01-28 | 李毅 | Collagen composite dressing and preparation method thereof |
| CN115075043A (en) * | 2022-06-16 | 2022-09-20 | 东华大学 | Preparation method of kapok-based dressing |
| CN115957368A (en) * | 2022-12-20 | 2023-04-14 | 重庆大学附属肿瘤医院 | Bacterial cellulose membrane for repairing skin wound and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101172164A (en) * | 2006-11-03 | 2008-05-07 | 中国科学院化学研究所 | Biopolymer nano tunica fibrosa material capable of being biological degraded and absorbed, preparing method and uses of the same |
| CN104558675A (en) * | 2014-10-10 | 2015-04-29 | 无锡贝迪生物工程有限公司 | Method for preparing microfiber hemostatic collagen sponge |
-
2017
- 2017-04-21 CN CN201710264339.6A patent/CN107126576A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101172164A (en) * | 2006-11-03 | 2008-05-07 | 中国科学院化学研究所 | Biopolymer nano tunica fibrosa material capable of being biological degraded and absorbed, preparing method and uses of the same |
| CN104558675A (en) * | 2014-10-10 | 2015-04-29 | 无锡贝迪生物工程有限公司 | Method for preparing microfiber hemostatic collagen sponge |
Non-Patent Citations (1)
| Title |
|---|
| 姚穆: "《纺织材料学 第4版》", 31 January 2015 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112675356A (en) * | 2020-12-23 | 2021-04-20 | 湖南工业大学 | Long-term-preservation hemostatic composition, preparation method thereof and preparation method of long-term-preservation antibacterial hemostatic mixture |
| CN113975448A (en) * | 2021-11-26 | 2022-01-28 | 李毅 | Collagen composite dressing and preparation method thereof |
| CN115075043A (en) * | 2022-06-16 | 2022-09-20 | 东华大学 | Preparation method of kapok-based dressing |
| CN115957368A (en) * | 2022-12-20 | 2023-04-14 | 重庆大学附属肿瘤医院 | Bacterial cellulose membrane for repairing skin wound and preparation method and application thereof |
| CN115957368B (en) * | 2022-12-20 | 2024-04-12 | 重庆大学附属肿瘤医院 | Bacterial cellulose membrane for repairing skin wounds and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103341209B (en) | Silk fibroin nanofiber membrane and preparation method thereof | |
| TWI398275B (en) | Skin wound dressing and manufacturing method thereof | |
| CN107126576A (en) | A kind of composite regenerated cellulosic wound dressings of kapok and preparation method thereof | |
| CN106039416B (en) | Chitosan-sericin compound bio bracket and its preparation method and application | |
| CN103893820B (en) | Fibroin albumen composite biological material support and preparation method thereof | |
| CN103756005B (en) | A kind of Composite collagen sponge and preparation method thereof | |
| CN105343926B (en) | A kind of scar plaster and preparation method thereof containing nano silica | |
| CN106132447A (en) | Bio-medical instrument | |
| CN102671236B (en) | Method for preparing nanofiber reinforcement hydrogel bionic artificial meniscus composite material | |
| CN106729984A (en) | A kind of Isin glue collagen repairs sponge and preparation method thereof | |
| CN103083720B (en) | Silk fibroin tube and preparation method thereof | |
| CN105903071A (en) | Cornea scaffold material and preparation method thereof and 3D printing method of cornea scaffold | |
| CN111393521A (en) | Extraction method of jellyfish collagen | |
| CN107441549A (en) | A kind of preparation method of collagen Heparan sulfate combine dressing | |
| CN106421903A (en) | Blue shark fishskin collagen periodontium guided regeneration nanofiber membrane and preparing method thereof | |
| CN107126579A (en) | A kind of former protein sponge of Yak-skin Gelatin acted on quick-acting haemostatic powder and its preparation method and application | |
| CN105833330B (en) | A kind of preparation method of bamboo based nano-fiber element collagen composite material | |
| Bao et al. | Agar/collagen membrane as skin dressing for wounds | |
| WO2022028105A1 (en) | Bionic composite bone scaffold and preparation method therefor | |
| CN106924800A (en) | A kind of regenerated cellulose wound dressing and preparation method thereof | |
| CN109602958A (en) | A kind of artificial skin and preparation method thereof | |
| CN108822335A (en) | A kind of composite membrane and its preparation method and application | |
| CN114404648A (en) | Preparation method of degradable antibacterial hemostatic hydrogel for promoting diabetic wound repair | |
| CN102836462A (en) | Preparation method for collagen tissue engineering skin | |
| CN113877001A (en) | Silk fibroin composite gel for injection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170905 |
|
| RJ01 | Rejection of invention patent application after publication |