CN102671236B - Method for preparing nanofiber reinforcement hydrogel bionic artificial meniscus composite material - Google Patents
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Abstract
A method for preparing a nanobacteria cellulose reinforcement hydrogel bionic meniscus composite material belongs to the technical field of biomedical materials or biocomposite, and is characterized in that a cellulose membrane is pretreated to control the water content of the cellulose membrane, polyvinyl alcohol sol is enabled to permeate the fiber membrane through an immersion method, fiber composite reinforcement is then performed on the polyvinyl alcohol hydrogel through a lamination reinforcement method or a dispersion strengthening method, and finally, the fiber membrane reinforcement hydrogel bionic artificial meniscus material with good mechanical properties is obtained through a plurality of times of freezing and defreezing. The method uses the nanofiber membrane with excellent biocompatibility to reinforce the polyvinyl alcohol hydrogel, the mechanical properties and stability of the hydrogel are improved, the composite material can be enabled to completely satisfy various performance requirements of a bionic artificial meniscus, and is lower in production cost, the shape and size of the produced sample are easy to regulate, and the composite material has good biocompatibility. The material can also be used in the biomedical fields of artificial cartilages, artificial blood vessels, artificial skins and the like except the bionic artificial meniscus.
Description
Technical field
The invention belongs to bio-medical material or Biocomposite material technical field, particularly provide a kind of nanometer bacteria cellulose film to strengthen the preparation method of the bionical meniscus composite of hydrogel.
Background technology
In knee joint continuously in motion, meniscus plays the important effects such as transmitted load, buffering load, stable and lubricated joint of bearing.Therefore, the integrity of meniscal structure is to keeping the kneed functional most important meaning that has.But the region due to meniscal 2/3rds is the white area without blood supply area, once just cause sustaining damage without the white area meniscus of blood supply just can not self-healing for this.Traditional treatment is only paid close attention to damaging meniscal excision, finishing and shaping etc., although releasing patient's that can be of short duration slight illness, owing to having lost meniscal function, degeneration of joint accelerates, finally cause the generation of knee joint endoprosthesis inflammation, thereby affect people's orthobiosis.At present, mainly contain for the reparation means of human body knee joint meniscus injury, allograft, artificial prosthesis is implanted and is repaired and three kinds of methods of organizational project, but these three kinds of methods all exist deficiency separately.At present, less about the research report of artificial meniscus prosthese implantation repair materials both at home and abroad, one of them major reason lacks exactly can meet the special mechanical property requirements of meniscus, has the material system of good biocompatibility simultaneously.
At present, study and more utilize various materials to repair meniscal method and can play to a certain extent temporary transient substituted role as the autologous tissues such as the artificial meniscus prosthese of preparation and fat pad and tendon tissue such as poly-tetrem alkene net, silicon, carbon fiber, terylene implant restoration reparation or implant, but some clinical relevant issues need further optimization as: problems such as mechanical strength, the use of cytokine and the selection of stress stimulation of engineered meniscal ruggedness, biocompatibility, timbering material.For example, the artificial meniscus that silk fiber-collagen composite builds has good mechanical property, short-term can be implanted and repair meniscal function, but its zooperal result shows, the permanence operation of the artificial meniscus implantation repair materials being built by fibroin fiber and collagen is not good, life-time service can cause articular degeneration, causes arthritis.The people such as Japanology personnel Kobayashi have done with polyvinyl alcohol hydrogel (PVA-H) research that human body meniscus is implanted restoration, the viscoelasticity that the PVA-H of discovery higher capacity shows is similar to the meniscal viscoelasticity of the mankind, but the binding ability of itself and bone substrate is poor, and lack enough mechanical strengths, this has directly affected the fixing of cartilage, the recovery of reparation and function thereof.How to give the good biological activity connectivity of artificial meniscus and surface lubrication, make it have again excellent biomechanical property and durability simultaneously, this is the difficult problem all not solving both at home and abroad.
Summary of the invention
The object of this invention is to provide the preparation method that a kind of nanometer bacteria cellulose strengthens the bionical meniscus material of hydrogel, make the bionical meniscus material of composite aquogel of preparing there is good elastic modelling quantity and tensile strength, simultaneously preparation method is simple, cost of manufacture is low, nanometer bacteria cellulose rete bonds well mutually with hydrogel layer, under the condition of maintenance high-moisture percentage, its every mechanical property is brought up to close to real human body meniscus.
Technical solution of the present invention is: remove culture medium and other impurity on Bacterial cellulose (BC) film by pretreatment, using deionized water rinsing to pH value is 7, adopt the method for heated and stirred to prepare polyvinyl alcohol (PVA) solution, with infusion process or lay-up method preparation enhancing nanometer bacteria fibrous membrane, pass through again physics freeze-thaw method for several times, finally obtain having the BC film enhanced polyethylene alcohol hydrogel biomimetic prosthetic meniscus material of favorable comprehensive mechanical property.
Concrete preparation technology is as follows:
method one: lamination strengthens method:
The preparation of step 1, poly-vinyl alcohol solution.
Take a certain amount of average degree of polymerization and be 1750 ± 200 granule of polyvinyl alcohol, the proportioning that is 5%-50% according to mass fraction adds deionized water heated and stirred 1～2h at 80-120 DEG C;
In the water-bath of 50-80 DEG C, leave standstill 0.5-1h, remove bubble insulation in solution.
The pretreatment of step 2, enhancing nanometer bacteria cellulose (BC) film.
1. get nanometer bacteria cellulose film and be cut into certain size according to mould size, repeatedly rinse with clear water, remove the NaOH solution that striping surface medium and impurity are soaked in film 0.01 ~ 0.5mol/L again, at 80-100 DEG C, boil 5～60min, remove thalline and residual culture medium in liquid film, now film is creamy white translucent.
2. repeatedly rinse with distilled water, survey pH value with the light press mold of pH reagent paper, about 7.1-7.5, obtains pretreated nanometer BC film.
3. again by pretreated nanometer BC film placement platform, it is applied to certain pressure, obtaining water content is the BC film of 75-90%; 0.5-1h in the PVA solution that nanometer bacteria cellulose film immersion after treatment is made in step 1, pretreatment is complete.
Step 3, nanometer bacteria cellulose film strengthen the preparation of hydrogel artificial meniscus composite.
1. nanometer BC film good pretreatment is taken out, sprawl at the mold bottom of making in advance, ensure that it does not have fold, and there is no space with perfect contact of mold bottom;
2. will handle well and keep the PVA aqueous solution of 50-80 DEG C to pour into mould, and it is spread on bottom nanometer BC film uniformly, thickness be about 2-5mm;
3. take out again a nanometer BC film that has been cut to mold shape, according to the method for " 1 ", spread on the PVA aqueous solution that is evenly distributed on bottom nanometer BC film;
4. leave standstill a moment, treat that PVA solution temperature is down to 30-40 DEG C of left and right, PVA aqueous solution stickiness increases, and after infiltrating gradually in upper and lower two-layer nanometer BC film, repeat " 2 " and " 3 ", spread altogether 2 ~ 5 layers of nanometer bacteria cellulose film, every tunic interval 2-5mm, fill uniformly PVA solution at interval, as shown in " Fig. 1 " in brief description of the drawings.
5. put together with mould the composite material frame preparing into-50 ~ 0 DEG C of freezing 10-20h, then take out and place the 1-5h that at room temperature thaws, repeat just can obtain for 5-10 time having the biomimetic prosthetic meniscus material of some strength.
method two: disperse strengthens method:
The preparation of step 1, poly-vinyl alcohol solution.
Take a certain amount of average degree of polymerization and be 1750 ± 200 granule of polyvinyl alcohol, the proportioning that is 5%-50% according to mass fraction adds deionized water heated and stirred 1～2h at 80-120 DEG C;
In the water-bath of 50-80 DEG C, leave standstill 0.5-1h, remove bubble insulation in solution.
The pretreatment of step 2, nanometer bacteria cellulose.
Get Bacterial cellulose (BC) film and be cut into certain size according to mould size, repeatedly rinse with clear water, except striping surface medium and impurity; Film is soaked in to the NaOH solution of 0.01 ~ 0.5mol/L again, boils 5～60min at 80-100 DEG C, remove thalline and residual culture medium in liquid film, now film is creamy white translucent; Repeatedly rinse with distilled water, survey pH value with the light press mold of pH reagent paper, about 7.1-7.5, obtains BC film;
By on pretreated BC film placement platform, it is applied to certain pressure again, obtaining water content is the BC film of 75-90%.
Step 3, disperse shape strengthen the preparation of nanometer bacteria fibrous membrane
1. BC film complete pretreatment is taken out from deionized water, put into the beaker that mass fraction is the strong alkali solution of the NaOH of 5%-10% is housed, in the water-bath of 50-100 DEG C, heat 1-2 hour;
2. beaker is taken out and is cooled to room temperature, be placed on freezing 10-15 hour in the cold closet of-5 ~-10 DEG C, then take out to place and at room temperature thaw, place it in freezing 10-15 hour in the cold closet of-5 ~-10 DEG C, freeze-thaw is for several times until BC fibrous membrane becomes transparence completely so repeatedly again;
3. transparent BC fibrous membrane carefully being cleaned to neutral repeatedly with deionized water, is finally that neutral transparent BC fibrous membrane grinds by pH value;
4. the tiny BC fiber block grinding is carried out to ultrasonic dispersion certain hour with ultrasonic dispersion instrument, obtain cotton-shaped reinforcing fiber granule.
Step 4, disperse nanofiber strengthen the preparation of hydrogel artificial meniscus composite
1. the BC fiber after PVA granule and ultrasonic dispersion is carried out to mix according to the mass ratio of BC/PVA%=50-300%, the ratio that is 10-30% according to the mass percent of PVA gel solution is added deionized water, and in the water under high pressure bath of 100-120 DEG C, 1-2 hour is stirred in heating;
2. dissolve completely and after BC fiber is uniformly dispersed in PVA gel solution, in the thermostat water bath of 70-95 DEG C, leave standstill a period of time until PVA granule, removing the bubble bringing in whipping process;
3. mixed solution is cast in ready mould, it is evenly distributed in mould, middle residual without bubble and hole, leave standstill and treat that solution is cooled to room temperature.
4. put together with mould the composite material frame preparing into-50 ~ 0 DEG C of freezing 10-20h, then take out and place the 1-5h that at room temperature thaws, repeat just can obtain for 5-10 time having the biomimetic prosthetic meniscus material of some strength.
compared with prior art, tool of the present invention has the following advantages and beneficial effect:
The nanofiber that the present invention obtains strengthens hydrogel biomimetic prosthetic meniscus, there is high-moisture percentage, comprehensive mechanical property and excellent in stability, good biocompatibility, and preparation method is simple, the sample shape of preparation and the feature such as size easy-regulating is honest and clean, can meet the requirements of meniscus implanting prosthetic.Simultaneously, can be by regulating the concentration of poly-vinyl alcohol solution and the water content of BC film to control the combination between PVA layer and BC rete, and adjust comprehensive mechanical property by controlling the number of plies of compound BC film, the number of times of freeze-thaw and the time that BC film floods in PVA solution, to meet different demands.Prepare composite and biomimetic prosthetic meniscus, also can be used for the bio-medical fields such as artificial cartilage, artificial blood vessel, artificial skin except can be used for.
Brief description of the drawings
Fig. 1 nanometer bacteria cellulose film lay-up method strengthens biomimetic prosthetic meniscus material structure schematic diagram
Fig. 2 nanometer bacteria cellulose film disperse method strengthens biomimetic prosthetic meniscus material structure schematic diagram.
Fig. 3 nanometer BC fiber/PVA hydrogel interpenetrating network scanning electron microscope (SEM) photograph.
Fig. 4 nanometer bacteria cellulose film strengthens the biomimetic prosthetic meniscus that hydrogel composite material is produced.
Detailed description of the invention
Example 1
By the commercially available Bacterial cellulose sample that has bacteria cellulose film clip that acetobacter xylinum makes to become 6 × 10cm.
Step 1, take the PVA of certain mass, the requirement that is 15% according to mass fraction adds a certain amount of deionized water.Heated and stirred 1.5h in the water-bath of 80 DEG C, allows PVA granule fully dissolve, then leaves standstill 0.5h in the water-bath of 60 DEG C, removes bubble insulation;
Step 2, get bacteria cellulose film clear water and repeatedly rinse, except striping surface medium and impurity.Film is soaked in to the NaOH solution of 0.1mol/L again, boils 20min at I00 DEG C, remove thalline and residual culture medium in liquid film, now film is creamy white translucent.Then repeatedly rinse with distilled water, with the light press mold survey of pH reagent paper pH value, approximately 7.2;
Step 3, by pretreated BC film, lie on smooth flat board, then put a flat board on BC film, on flat board, apply the counterweight of 10KG, persistent period 0.5h, extrudes the part moisture in BC film, is then placed in cold storage refrigerator cold preservation for subsequent use;
Step 4, by BC film immersion in the PVA solution preparing, persistent period 5min;
Step 5, BC film is taken out from PVA solution, spread 2 ~ 3 layers of BC film at rectangular mould according to the method for laying, then in mould, inject a certain amount of PVA solution;
Step 6, put the composite preparing into-50 ~ 0 DEG C of freezing 10-20h, then take out and place the 1-5h that at room temperature thaws, repeat just can obtain for 5-10 time having the biomimetic prosthetic meniscus material of some strength.
Example 2
By the commercially available nanometer bacteria cellulose sample that has nanometer bacteria cellulose film clip that acetobacter xylinum makes to become 6 × 10cm.
Step 1, take the PVA of certain mass, the requirement that is 20% according to mass fraction adds a certain amount of deionized water.Heated and stirred 1.5h in the water-bath of 80 DEG C, allows PVA granule fully dissolve, then leaves standstill 0.5h in the water-bath of 60 DEG C, removes bubble insulation;
Step 2, get nanometer bacteria cellulose film clear water and repeatedly rinse, except striping surface medium and impurity.Film is soaked in to the NaOH solution of 0.1mol/L again, boils 20min at I00 DEG C, remove thalline and residual culture medium in liquid film, now film is creamy white translucent.Then repeatedly rinse with distilled water, with the light press mold survey of pH reagent paper pH value, approximately 7.2;
Step 3, by pretreated nanometer BC film, lie on smooth flat board, flat board is placed on to the 0.5h of shady and cool ventilation place, be then placed in cold storage refrigerator cold preservation for subsequent use;
Step 4, nanometer BC film is taken out from cold closet, spread 2 ~ 3 layers of BC film at rectangular mould according to the method for laying, then in mould, inject a certain amount of PVA solution;
Step 5, put the composite preparing into-50 ~ 0 DEG C of freezing 10-20h, then take out and place the 1-5h that at room temperature thaws, repeat just can obtain for 5-10 time having the biomimetic prosthetic meniscus material of some strength.
Example 3
By the commercially available nanometer bacteria cellulose sample that has nanometer bacteria cellulose film clip that acetobacter xylinum makes to become 6 × 10cm.
Step 1, take the PVA of certain mass, the requirement that is 25% according to mass fraction adds a certain amount of deionized water.Heated and stirred 1.5h in the water-bath of 80 DEG C, allows PVA granule fully dissolve, then leaves standstill 0.5h in the water-bath of 60 DEG C, removes bubble insulation;
Step 2, get nanometer bacteria cellulose film clear water and repeatedly rinse, except striping surface medium and impurity.Film is soaked in to the NaOH solution of 0.1mol/L again, boils 20min at I00 DEG C, remove thalline and residual culture medium in liquid film, now film is creamy white translucent.Then repeatedly rinse with distilled water, with the light press mold survey of pH reagent paper pH value, approximately 7.2;
Step 3, by pretreated nanometer BC film, lie on smooth flat board, with the part moisture in absorbent paper sucking-off nanometer BC film, be then placed in cold storage refrigerator cold preservation for subsequent use;
Step 4, nanometer BC film is taken out from cold closet, spread 2 ~ 3 layers of BC film at rectangular mould according to the method for laying, then in mould, inject a certain amount of PVA solution;
Step 5, put the composite preparing into-50 ~ 0 DEG C of freezing 10-20h, then take out and place the 1-5h that at room temperature thaws, repeat just can obtain for 5-10 time having the biomimetic prosthetic meniscus material of some strength.
Example 4
By the commercially available Bacterial cellulose sample that has nanometer bacteria cellulose film clip that acetobacter xylinum makes to become 6 × 10cm.
Step 1, get nanometer bacteria cellulose film and be cut into certain size according to mould size, repeatedly rinse with clear water, except striping surface medium and impurity; Film is soaked in to the NaOH solution of 0.1mol/L again, boils 20min at 80-100 DEG C, remove thalline and residual culture medium in liquid film, now film is creamy white translucent; Repeatedly rinse with distilled water, survey pH value with the light press mold of pH reagent paper, approximately 7.2, obtain nanometer BC film; By on pretreated nanometer BC film placement platform, it is applied to certain pressure again, obtaining water content is the nanometer BC film of 75-90%;
Step 2, the nanometer BC fibrous membrane after alkali treatment, purification is taken out from deionized water, put into the beaker that mass fraction is the strong alkali solution of the NaOH of 5%-10% is housed, in the water-bath of 80 DEG C, heat 1.5 hours; Beaker is taken out and is cooled to room temperature, be placed in the cold closet of-8 DEG C freezing 12 hours, then take out to place and at room temperature thaw, then place it in the cold closet of-8 DEG C freezing 12 hours, freeze-thaw is for several times until BC fibrous membrane becomes transparence completely so repeatedly; Transparent BC fibrous membrane is carefully cleaned to neutral repeatedly with deionized water, is finally that neutral transparent BC fibrous membrane grinds by pH value; The tiny BC fiber block grinding is carried out to ultrasonic dispersion certain hour with ultrasonic dispersion instrument, obtain cotton-shaped reinforcing fiber granule.
Step 3, the nanometer BC fiber after PVA granule and ultrasonic dispersion is carried out to mix according to mass ratio 1:1, the ratio that is 10-30% according to the mass percent of PVA gel solution is added deionized water, in the water under high pressure bath of 120 DEG C, heats and stirs 2 hours; Dissolve completely and after nanometer BC fiber is uniformly dispersed in PVA gel solution, in the thermostat water bath of 70-95 DEG C, leave standstill a period of time until PVA granule, removing the bubble bringing in whipping process; Mixed solution is cast in ready mould, uses Glass rod that it is evenly distributed in mould, middle residual without bubble and hole, leave standstill and treat that solution is cooled to room temperature.Put together with mould the composite material frame preparing into-20 DEG C of freezing 10-20h, then take out and place the 3h that at room temperature thaws, repeat 5-10 time and just can obtain biomimetic prosthetic meniscus material.
Claims (4)
1.
oneplant the preparation method of the bionical meniscus material of nanometer bacteria cellulose enhancing hydrogel, it is characterized in that removing culture medium and other impurity on nanometer bacteria cellulose BC by pretreatment, using deionized water rinsing to pH value is 7, adopt the method for heated and stirred to prepare PVAC polyvinylalcohol solution, adopt the preparation of lay-up method technique to strengthen nanometer bacteria fibrous membrane, pass through again physics freeze-thaw method for several times, finally obtain having the nanometer BC film enhanced polyethylene alcohol hydrogel biomimetic prosthetic meniscus material of favorable comprehensive mechanical property; Concrete steps are as follows:
The preparation of step 1, poly-vinyl alcohol solution:
Take a certain amount of average degree of polymerization and be 1750 ± 200 granule of polyvinyl alcohol, the proportioning that is 5%-50% according to mass fraction adds deionized water; Heated and stirred 1～2h at 80-120 DEG C; In the water-bath of 50-80 DEG C, leave standstill 0.5-1h, remove bubble insulation in solution;
The pretreatment of step 2, enhancing nanometer bacteria cellulose:
(1). get nanometer bacteria cellulose film and be cut into certain size according to mould size, repeatedly rinse with clear water, remove the NaOH solution that striping surface medium and impurity are soaked in film 0.01 ~ 0.5mol/L again, at 80-100 DEG C, boil 5～60min, remove thalline and residual culture medium in liquid film, now film is creamy white translucent;
(2). repeatedly rinse with distilled water, survey pH value 7 with the light press mold of pH reagent paper, obtain pretreated nanometer BC film;
(3). by pretreated nanometer BC film placement platform, it is applied to certain pressure again, obtaining water content is the nanometer BC film of 75-90%; 0.5-1h in the PVA solution that nanometer bacteria cellulose film immersion after treatment is made in step 1, pretreatment is complete;
Step 3, nanometer bacteria cellulose film strengthen the preparation of hydrogel artificial meniscus composite:
(1) nanometer BC film good pretreatment is taken out, sprawl at the mold bottom of making in advance, ensure that it does not have fold, and there is no space with perfect contact of mold bottom;
(2) will handle well and keep the PVA aqueous solution of 50-80 DEG C to pour into mould, use Glass rod that it is spread on bottom nanometer BC film uniformly, thickness be 2-5mm;
(3) take out again a good nanometer BC film of pretreatment that has been cut to mold shape, according to the method for this step " (1) ", spread on the PVA aqueous solution that is evenly distributed on bottom nanometer BC film;
(4) leave standstill a moment, treat that PVA solution temperature is down to 30-40 DEG C, PVA aqueous solution stickiness increases, and after infiltrating gradually in upper and lower two-layer nanometer BC film, repeat this step " (2) " and " (3) ", spread altogether 2 ~ 5 layers of nanometer bacteria cellulose film, every tunic interval 2-5mm, fills PVA solution uniformly at interval;
(5) put together with mould the composite material frame preparing into-50 ~ 0 DEG C of freezing 10-20h, then take out and place the 1-5h that at room temperature thaws, repeat just can obtain for 5-10 time having the biomimetic prosthetic meniscus material of some strength.
2. a kind of nanometer bacteria cellulose strengthens the preparation method of the bionical meniscus material of hydrogel as claimed in claim 1, it is characterized in that adopting disperse to strengthen the preparation of method technique and strengthen nanometer bacteria fibrous membrane, through physics freeze-thaw method for several times, finally obtain having the BC film enhanced polyethylene alcohol hydrogel biomimetic prosthetic meniscus material of favorable comprehensive mechanical property; Concrete steps are as follows:
The pretreatment of step 1, nanometer bacteria cellulose:
Get nanometer bacteria cellulose BC film and be cut into certain size according to mould size, repeatedly rinse with clear water, except striping surface medium and impurity; Film is soaked in to the NaOH solution of 0.01 ~ 0.5mol/L again, boils 5～60min at 80-100 DEG C, remove thalline and residual culture medium in liquid film, now film is creamy white translucent; Repeatedly rinse with distilled water, survey pH value 7 with the light press mold of pH reagent paper, obtain BC film;
By on pretreated BC film placement platform, it is applied to certain pressure again, obtaining water content is the BC film of 75-90%;
Step 2, disperse shape strengthen the preparation of nanometer bacteria fibrous membrane:
(1) nanometer BC film complete pretreatment is taken out, put into the beaker that mass fraction is the strong alkali solution of the NaOH of 5%-10% is housed, in the water-bath of 50-100 DEG C, heat 1-2 hour;
(2) beaker is taken out and is cooled to room temperature, be placed on freezing 10-15 hour in the cold closet of-5 ~-10 DEG C, then take out to place and at room temperature thaw, place it in freezing 10-15 hour in the cold closet of-5 ~-10 DEG C, freeze-thaw is for several times until nanometer BC fibrous membrane becomes transparence completely so repeatedly again;
(3) transparent BC fibrous membrane carefully being cleaned to neutral repeatedly with deionized water, is finally that neutral transparent nanometer BC fibrous membrane grinds by pH value;
(4) the tiny BC fiber block grinding is carried out to ultrasonic dispersion certain hour with ultrasonic dispersion instrument, obtain cotton-shaped reinforcing fiber granule;
Step 3, disperse nanofiber strengthen the preparation of hydrogel artificial meniscus composite:
(1) the BC fiber after PVA granule and ultrasonic dispersion is carried out to mix according to the mass ratio of BC/PVA%=50-300%, the ratio that is 10-30% according to the mass percent of PVA gel solution is added deionized water, and in the water under high pressure bath of 100-120 DEG C, 1-2 hour is stirred in heating;
(2) dissolve completely and after BC fiber is uniformly dispersed in PVA gel solution, in the thermostat water bath of 70-95 DEG C, leave standstill a period of time until PVA granule, removing the bubble bringing in whipping process;
(3) mixed solution is cast in ready mould, it is evenly distributed in mould, middle residual without bubble and hole, leave standstill and treat that solution is cooled to room temperature;
(4) put together with mould the composite material frame preparing into-50 ~ 0 DEG C of freezing 10-20h, then take out and place the 1-5h that at room temperature thaws, repeat just can obtain for 5-10 time having the biomimetic prosthetic meniscus material of some strength.
3. a kind of nanometer bacteria cellulose according to claim 1 strengthens the preparation method of the bionical meniscus material of hydrogel, it is characterized in that, the fibrous membrane that employing has multiple-void structure strengthens hydrogel, the cellulose membrane of purchase is carried out to pretreatment, and repeatedly rinse at deionized water, remove the impurity of substrate surface, the PVA solution that is 10% ~ 30% by water bath heating preparation quality percent again, then prepares the artificial bionic meniscus material with excellent mechanical performances and biocompatibility by dispersion-strengtherning method and by the freezing freezing process of physics.
4. a kind of nanometer bacteria cellulose according to claim 2 strengthens the preparation method of the bionical meniscus material of hydrogel, it is characterized in that, the fibrous membrane that employing has multiple-void structure strengthens hydrogel, the cellulose membrane of purchase is carried out to pretreatment, and repeatedly rinse at deionized water, remove the impurity of substrate surface, the PVA solution that is 10% ~ 30% by water bath heating preparation quality percent again, then prepares the artificial bionic meniscus material with excellent mechanical performances and biocompatibility by stacked reinforcement and by the freezing freezing process of physics.
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| 层状纳米纤维素膜/PVA 复合水凝胶的制备与力学性能研究;谭珏 等;《高分子学报》;20120420(第4期);摘要、正文第351页第2－3段、第1节实验部分，图1以及第2.2节第1段右栏 * |
| 谭珏 等.层状纳米纤维素膜/PVA 复合水凝胶的制备与力学性能研究.《高分子学报》.2012,(第4期),摘要、正文第351页第2－3段、第1节实验部分，图1以及第2.2节第1段右栏. |
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|---|---|
| CN102671236A (en) | 2012-09-19 |
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