CN106071639A - 一种芒果仁抗氧化添加剂及其制备方法 - Google Patents
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Abstract
本发明公开了一种芒果仁抗氧化添加剂及其制备方法。本发明的芒果仁抗氧化添加剂包括如下组分：芒果仁提取物，菊花粉，大蒜粉，乳酸钙，维生素A，维生素E，维生素C，维生素D。本发明取材天然，营养价值高，无毒副作用，能够抗凝血和降血脂、增强免疫细胞、抗肿瘤，具有很好的抗氧化作用，应用范围广。
Description
技术领域
本发明属于食品添加剂技术领域，具体涉及一种芒果仁抗氧化添加及其制备方法。
背景技术
现有的食品抗氧化添加剂主要有化学食品抗氧化添加剂和天然食品抗氧化添加剂两大类，其中，化学食品抗氧化添加剂效果良好，但是存在不同程度的毒副作用等缺点，长期使用或者食用过量对身体造成损伤。人工化学食品抗氧化添加剂是指用人工化学合成方法所制得的。与此同时，大量的研究报告指出，几乎所有的化学食品抗氧化添加剂都不能向人体提供营养物质，某些化学食品抗氧化添加剂甚至会危害人体健康。
发明内容
本发明的目的在于克服现有技术缺陷，提供一种芒果仁抗氧化添加剂。
本发明的另一目的在于提供该芒果仁抗氧化添加剂的制备方法。
本发明的技术方案如下：
一种芒果仁抗氧化添加剂，包括如下的组分：
芒果仁提取物10-20克
菊花粉0.3-0.5克
大蒜粉0.2-0.5克
乳酸钙0.2-0.5克
维生素A0.4-0.6克
维生素E0.1-0.3克
维生素C1-3克
维生素D0.1-0.2克
本发明的另一技术方案如下：
一种上述芒果仁抗氧化添加剂的制备方法，包括如下步骤：
（1）将芒果仁依次经乙醇水溶液热回流浸提、乙酸乙酯萃取、聚乙二醇20000层析柱层析、聚酰胺粉层析、洗脱液乙醇重结晶和干燥后，制得所述芒果仁提取物；
（2）将上述芒果仁提取物10-20克，菊花粉0.3-0.5克，大蒜粉0.2-0.5克，乳酸钙0.2-0.5克，维生素A0.4-0.6克，维生素E0.1-0.3克，维生素C1-3克，维生素D0.1-0.2克混合搅拌均匀，干燥后即得所述芒果仁抗氧化添加剂。
在本发明的一个优选实施方案中，所述步骤（1）包括如下步骤：
a、将上述芒果仁干燥粉碎后加入乙醇水溶液于50-60℃热回流浸提20-30分钟，并过滤，得浸提液；
b、将上述浸提液用乙酸乙酯进行萃取，得萃取液；
c、将上述萃取液用聚乙二醇20000柱进行柱层析，再用浓度为10-20%的乙醇水溶液洗脱，得第一级洗脱液；
d、将上述第一级洗脱液用聚酰胺粉作为吸附剂进行层析，再用醋酸水溶液的混合液洗脱，得第二级洗脱液；
e、将上述第二级洗脱液用乙醇进行重结晶，干燥后，即得所述芒果仁提取物。
在本发明的一个优选实施方案中，所述步骤（1）的步骤a中，乙醇水溶液的浓度为20-30%，该乙醇水溶液总重量为干燥粉碎后的芒果仁重量的2-3倍。
在本发明的一个优选实施方案中，所述步骤（1）的步骤d中，所述聚酰胺粉为200目，所述醋酸水溶液的浓度为0.2-0.5%。
本发明的有益效果是：
1、本发明的抗氧化添加剂中含有芒果仁，取材天然，营养价值高，无毒副作用，能够抗凝血和降血脂、增强免疫细胞、抗肿瘤，具有很好的抗氧化作用，应用范围广；
2、本发明的抗氧化添加剂中的维生素E是细胞膜内重要的抗氧化物和膜稳定剂，维持肌肉组织的正常机构和代谢，特别是在肌肉收缩期间的能量供给和钙离子摄取和释放有着重要的作用；
3、本发明的抗氧化添加剂中的维生素C可明显降低运动诱导的氧化应激。
具体实施方式
以下通过具体实施方式对本发明的技术方案进行进一步的说明和描述。
实施例1
（1）制备芒果仁提取物：
a、将1kg芒果仁干燥粉碎后加入2kg20%乙醇水溶液，于50℃热回流浸提20分钟，并过滤浓缩，得浸提液；
b、将上述浸提液用乙酸乙酯进行萃取，得萃取液；
c、将上述萃取液用聚乙二醇20000柱进行柱层析，再用浓度为10%的乙醇水溶液洗脱，得第一级洗脱液；
d、将上述第一级洗脱液用200目的聚酰胺粉作为吸附剂进行层析，再用0.5%醋酸水溶液洗脱，得第二级洗脱液；
e、将上述第二级洗脱液用乙醇进行重结晶，干燥后，即得所述芒果仁提取物；
（2）将上述芒果仁提取物10克、菊花粉0.3克、大蒜粉0.2克、乳酸钙0.2克，维生素A0.4克，维生素E0.1克，维生素C1克，维生素D0.1克混合搅拌均匀，干燥后即得所述芒果仁抗氧化添加剂。
实施例2
（1）制备芒果仁提取物：
a、将1kg芒果仁干燥粉碎后加入2.5kg25%乙醇水溶液，于55℃热回流浸提25分钟，并过滤浓缩，得浸提液；
b、将上述浸提液用乙酸乙酯进行萃取，得萃取液；
c、将上述萃取液用聚乙二醇20000柱进行柱层析，再用浓度为15%的乙醇水溶液洗脱，得第一级洗脱液；
d、将上述第一级洗脱液用200目的聚酰胺粉作为吸附剂进行层析，再用0.3%醋酸水溶液洗脱，得第二级洗脱液；
e、将上述第二级洗脱液用乙醇进行重结晶，干燥后，即得所述芒果仁提取物；
（2）将上述芒果仁提取物15克、菊花粉0.4克、大蒜粉0.3克、乳酸钙0.3克，维生素A0.5克，维生素E0.2克，维生素C2克，维生素D0.2克混合搅拌均匀，干燥后即得所述芒果仁抗氧化添加剂。
实施例3
（1）制备芒果仁提取物：
a、将1kg芒果仁干燥粉碎后加入3kg30%乙醇水溶液，于60℃热回流浸提30分钟，并过滤浓缩，得浸提液；
b、将上述浸提液用乙酸乙酯进行萃取，得萃取液；
c、将上述萃取液用聚乙二醇20000柱进行柱层析，再用浓度为20%的乙醇水溶液洗脱，得第一级洗脱液；
d、将上述第一级洗脱液用200目的聚酰胺粉作为吸附剂进行层析，再用0.5%醋酸水溶液洗脱，得第二级洗脱液；
e、将上述第二级洗脱液用乙醇进行重结晶，干燥后，即得所述芒果仁提取物；
（2）将上述芒果仁提取物20克、菊花粉0.5克、大蒜粉0.5克、乳酸钙0.5克，维生素A0.6克，维生素E0.3克，维生素C3克，维生素D0.2克混合搅拌均匀，干燥后即得所述芒果仁抗氧化添加剂。
以上所述，仅为本发明的较佳实施例而已，故不能依此限定本发明实施的范围，即依本发明专利范围及说明书内容所作的等效变化与修饰，皆应仍属本发明涵盖的范围内。
Claims (4)
1.一种芒果仁抗氧化添加剂，其特征在于：包括如下重量的组分：
芒果仁提取物10-20克
菊花粉0.3-0.5克
大蒜粉0.2-0.5克
乳酸钙0.2-0.5克
维生素A0.4-0.6克
维生素E0.1-0.3克
维生素C1-3克
维生素D0.1-0.2克
上述芒果仁提取物的提取方法如下：
a、将上述芒果仁干燥粉碎后加入乙醇水溶液于50-60℃热回流浸提20-30分钟，并过滤，得浸提液；
b、将上述浸提液用乙酸乙酯进行萃取，得萃取液；
c、将上述萃取液用聚乙二醇20000柱进行柱层析，再用浓度为10-20%的乙醇水溶液洗脱，得第一级洗脱液；
d、将上述第一级洗脱液用聚酰胺粉作为吸附剂进行层析，再用醋酸水溶液洗脱，得第二级洗脱液；
e、将上述第二级洗脱液用乙醇进行重结晶，干燥后，即得所述芒果仁提取物。
2.一种权利要求1所述的芒果仁抗氧化添加剂的制备方法，其特征在于：包括如下步骤：
（1）将芒果仁依次经乙醇水溶液热回流浸提、乙酸乙酯萃取、聚乙二醇20000柱层析、聚酰胺粉层析、洗脱液乙醇重结晶和干燥后，制得所述芒果仁提取物，该步骤包括如下步骤：
a、将上述芒果仁干燥粉碎后加入乙醇水溶液于50-60℃热回流浸提20-30分钟，并过滤，得浸提液；
b、将上述浸提液用乙酸乙酯进行萃取，得萃取液；
c、将上述萃取液用聚乙二醇20000柱进行柱层析，再用浓度为10-20%的乙醇水溶液洗脱，得第一级洗脱液；
d、将上述第一级洗脱液用聚酰胺粉作为吸附剂进行层析，再用醋酸水溶液的混合液洗脱，得第二级洗脱液；
e、将上述第二级洗脱液用乙醇进行重结晶，干燥后，即得所述芒果仁提取物；
（2）将上述芒果仁提取物10-20克，菊花粉0.3-0.5克，大蒜粉0.2-0.5克，乳酸钙0.2-0.5克，维生素A0.4-0.6克，维生素E0.1-0.3克，维生素C1-3克，维生素D0.1-0.2克混合搅拌均匀，干燥后即得所述芒果仁抗氧化添加剂。
3.如权利要求2所述的一种芒果仁抗氧化添加剂的制备方法，其特征在于：所述步骤（1）的步骤a中，乙醇水溶液的浓度为20-30%，该乙醇水溶液总重量为干燥粉碎后的芒果仁重量的2-3倍。
4.如权利要求2所述的一种芒果仁抗氧化添加剂的制备方法，其特征在于：所述步骤（1）的步骤d中，所述聚酰胺粉为200目，所述醋酸水溶液的浓度为0.2-0.5%。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107006837A (zh) * | 2017-03-27 | 2017-08-04 | 湖北文理学院 | 一种桑叶环糊精抗氧化添加剂及其制备方法 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102657365A (zh) * | 2012-05-28 | 2012-09-12 | 合浦果香园食品有限公司 | 一种新鲜芒果核的保鲜方法 |
| CN103445140A (zh) * | 2013-07-15 | 2013-12-18 | 闽南师范大学 | 一种白背毛木耳抗氧化添加剂及其制备方法 |
| CN105558927A (zh) * | 2015-12-30 | 2016-05-11 | 湖北文理学院 | 一种苹果籽抗氧化添加剂及其制备方法 |
| CN105639400A (zh) * | 2015-12-30 | 2016-06-08 | 湖北文理学院 | 一种香蕉皮抗氧化添加剂及其制备方法 |
-
2016
- 2016-06-13 CN CN201610413150.4A patent/CN106071639A/zh active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102657365A (zh) * | 2012-05-28 | 2012-09-12 | 合浦果香园食品有限公司 | 一种新鲜芒果核的保鲜方法 |
| CN103445140A (zh) * | 2013-07-15 | 2013-12-18 | 闽南师范大学 | 一种白背毛木耳抗氧化添加剂及其制备方法 |
| CN105558927A (zh) * | 2015-12-30 | 2016-05-11 | 湖北文理学院 | 一种苹果籽抗氧化添加剂及其制备方法 |
| CN105639400A (zh) * | 2015-12-30 | 2016-06-08 | 湖北文理学院 | 一种香蕉皮抗氧化添加剂及其制备方法 |
Non-Patent Citations (3)
| Title |
|---|
| 向海艳 等: "芒果废弃物不同部位乙醇提取物DPPH自由基清除能力的研究", 《安徽农业科学》 * |
| 杨尔滨: "《健康大挑战》", 31 January 2007 * |
| 蔡干 等: "《有机精细化学品实验》", 30 November 1997 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107006837A (zh) * | 2017-03-27 | 2017-08-04 | 湖北文理学院 | 一种桑叶环糊精抗氧化添加剂及其制备方法 |
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