CN106070632A - A kind of Carlina acaulis Yoghourt and preparation method thereof - Google Patents

A kind of Carlina acaulis Yoghourt and preparation method thereof Download PDF

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CN106070632A
CN106070632A CN201610505951.3A CN201610505951A CN106070632A CN 106070632 A CN106070632 A CN 106070632A CN 201610505951 A CN201610505951 A CN 201610505951A CN 106070632 A CN106070632 A CN 106070632A
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carlina acaulis
obtains
ratio
yoghourt
preparation
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汤须崇
寇馨月
邓爱华
雷廷平
蔡婀娜
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Huaqiao University
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Huaqiao University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/133Fruit or vegetables

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Abstract

The invention discloses a kind of Carlina acaulis Yoghourt and preparation method thereof, Carlina acaulis stem and leaf extract is made lyophilized powder, and after milk mixing homogenizing, inoculation fermentation prepares Carlina acaulis Yoghourt, have the double health effect of Carlina acaulis and Yoghourt concurrently, comprehensive nutrition, the pure and fresh uniqueness of local flavor, delicate mouthfeel, sour-sweet moderate, all-ages, cholesterol reducing is made good use of, and is especially suitable for hyperlipemic patients and long-term alcohol personage drinks.

Description

A kind of Carlina acaulis Yoghourt and preparation method thereof
Technical field
The invention belongs to food processing technology field, be specifically related to a kind of Carlina acaulis Yoghourt and preparation method thereof.
Background technology
Carlina acaulis, has another name called chrysanthemum Ji, Cynara scolymus L (Cyhara cardunculus.), France Bulbus Lilii, Flos Nelumbinis Bulbus Lilii herbaceos perennial.Originate in Mediterranean, Also a small amount of cultivation is had on China Shanghai, Zhejiang, Hunan, Yunnan and other places.Carlina acaulis blade contains cynarin, chlorogenic acid, flavonoid The composition that compound etc. are useful to human body, is a kind of health-care vegetable rich in high nutritive value, often eats and can protect Liver and kidney, increasing The dirty toxin expelling of kidney tonifying, prevent arteriosclerosis, protection cardiovascular.Carlina acaulis is with alabastrum for food, and leaf, stem, holder etc. are not owing to being had Effect utilization and abandon, cause the wasting of resources.Therefore, from stem, leaf, extract the beneficiating ingredients such as cynarin and contribute to combining of resource Close and utilize, there is in terms of food processing good prospect.
Yoghourt is with fresh milk as raw material, adds a certain proportion of sucrose, after high temperature sterilization cools down, adds A kind of milk product that pure lactic acid bacteria is cultivated, taste is sour-sweet fine and smooth, nutritious.Sweat makes sugar, protein in milk have About 20% is hydrolyzed into as little molecule, fat content general 3%~5% in milk.After fermented, the fatty acid in Yoghourt can 2 times are increased than raw material.These changes make Yoghourt more easy to digest and absorb, and the utilization rate of various nutrients is improved.It is a kind of The food integrate nutrition, keeping healthy.Yoghourt can suppress the growth of intestinal putrefaction bacteria, possibly together with suppressing internal synthetic cholesterol The active substance of reductase.The most edible Yoghourt, has additional nutrients, and prevents and treats arteriosclerosis, effect of reduction cholesterol.But by There is presently no the report that Carlina acaulis is combined with Yoghourt and makes health care yoghourt.
Summary of the invention
In place of it is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of Carlina acaulis Yoghourt and preparation side thereof Method, the Carlina acaulis Yoghourt obtained has the double health effect of Carlina acaulis and Yoghourt, and delicate mouthfeel, unique flavor concurrently, and the old and the young are all Preferably, it is especially suitable for hyperlipemic patients and long-term alcohol personage drinks.
One of the technical solution adopted for the present invention to solve the technical problems is:
A kind of preparation method of Carlina acaulis Yoghourt, including:
1) take the Carlina acaulis stem and leaf powder that particle diameter is 10~30 mesh, add the 55~70 of 7~10 times amount (mass volume ratio) DEG C water, 600~1000W microwave extractioies 80~120s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous absorption tree subsequently Fat is isolated and purified, Carlina acaulis extractum according to 1~4BV/h volume flow loading, 45~55% ethanol of 2~5BV (column volumes) Eluting, elution speed is 1~4BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.08~1.32 at 50 DEG C, freezing Being dried 48~75h, obtain Carlina acaulis lyophilized powder, wherein the content of cynarin is 4~6%;
2) according to the ratio that mass ratio is 8~12:100 of whole milk powder Yu water, whole milk powder is dissolved in 30~45 DEG C of temperature In water, addition 3~the sucrose of 12% (mass volume ratio), mixing, filter, sterilizing 12~20min, sterilizing at 85~100 DEG C Rapidly milk is cooled to 30~42 DEG C afterwards;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to mass volume ratio 1:45~200 Ratio mixing, filter, filtrate is heated to 75~90 DEG C, homogeneous micronization under 18~25MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 5.8~6.5, then through 122~135 DEG C, 1~3s High-temperature short-time sterilization, is cooled to the most rapidly 38~42 DEG C;By Lactobacillus bulgaricus and streptococcus thermophilus with mass ratio for 1: After the ratio mixing of 0.8~1.2, according to inoculum concentration 1.5~9% inoculation, ferment at constant temperature 3~24h at 41~43 DEG C subsequently;
5) in step 4) tunning that obtains accesses 0.1~the stabilizer of 0.6% (mass volume ratio), such as fruit Glue, gelatin, modified starch, agar, lactoprotein etc., after-ripening 8~12h at 3~6 DEG C, get product.
In one embodiment: described step 2) in, sucrose addition is 8~10%.
In one embodiment: described step 4) in, inoculum concentration is 6~8%.
In one embodiment: described step 5) in, the addition of stabilizer is 0.4~0.5%.
The two of the technical solution adopted for the present invention to solve the technical problems are:
A kind of Carlina acaulis Yoghourt prepared according to above-mentioned preparation method.
The technical program is compared with background technology, and it has the advantage that
The present invention combines microwave extraction and macroporous resin column separating purification, cynarin purity in the Carlina acaulis lyophilized powder obtained Higher, utilization rate is high;Again it is combined with Yoghourt and makes Yoghourt, be allowed to have concurrently the health-care effect of Carlina acaulis and Yoghourt, without layering Phenomenon, delicate mouthfeel, sour-sweet moderate, the pure and fresh uniqueness of local flavor, nutritious comprehensively, all-ages.Carlina acaulis has hepatoprotective And effect of reduction cholesterol, improve cholesterol reducing effect after making Yoghourt further, be particularly suitable for hyperlipemic patients and length The phase personage that drinks drinks.
Detailed description of the invention
Present disclosure is illustrated below by embodiment:
Embodiment 1
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 6%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:50 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 4% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.2% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Embodiment 2
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 8%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:100 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 6% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.3% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Embodiment 3
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 10%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:150 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 8% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.4% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Embodiment 4
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 6%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:150 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 8% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.3% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Embodiment 5
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 8%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:180 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 5% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.3% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Embodiment 6
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 10%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:100 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 4% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.2% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Embodiment 7
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 6%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:200 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 6% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.4% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Embodiment 8
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 8%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:100 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 8% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.2% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Embodiment 9
1) clean Carlina acaulis stem and leaf is dried, is crushed to 20 mesh, add 60 DEG C of water of 8 times amount, 700W microwave extraction 90s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified: the LSA-21 good by pretreatment Resin loads in glass chromatograph, and Carlina acaulis extractum is according to the volume flow loading of 2BV/h, 50% ethanol elution of 3BV, eluting speed Degree is 2BV/h, and the eluent obtained is condensed into the clear paste of relative density 1.10~1.30 at 50 DEG C, lyophilization 72h, obtains court Fresh Ji lyophilized powder, wherein the content of cynarin is 5%;
2) according to the ratio that mass ratio is 9:100 of whole milk powder Yu water, whole milk powder is dissolved in 40 DEG C of warm water, adds The sucrose of 10%, mixing, filter, sterilizing 15min at 90 DEG C, after sterilizing, rapidly milk is cooled to 40 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:200 Example mixes, and filters, and filtrate is heated to 80 DEG C, homogeneous micronization under 20MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 6.0, then through 125 DEG C, 2s high-temperature short-time sterilization, It is cooled to the most rapidly 40 DEG C;After Lactobacillus bulgaricus is mixed with the streptococcus thermophilus ratio with mass ratio as 1:1, according to Inoculum concentration 4% is inoculated, subsequently ferment at constant temperature 4h at 42 DEG C;
5) in step 4) tunning that obtains accesses 0.3% stabilizer, at 4 DEG C, after-ripening 10h, gets product;Put Stored refrigerated in 4 DEG C of refrigerators.
Experimental example
This experimental example is investigated respectively in the case of remaining factor is identical, different sucrose addition, inoculation amounts With the stabilizing agent dosage impact on Quality of Yoghourt, method for preparing sour milk is with reference to above-described embodiment, and result is if table 1 below is to shown in table 3.
The impact on Quality of Yoghourt of the table 1 sucrose addition
Curdled milk time and Quality of Yoghourt are affected by table 2 inoculation amount
The impact on Quality of Yoghourt of table 3 stabilizing agent dosage
The above, only present pre-ferred embodiments, therefore the scope that the present invention implements can not be limited according to this, i.e. depend on The equivalence change that the scope of the claims of the present invention and description are made with modify, all should still belong in the range of the present invention contains.

Claims (5)

1. the preparation method of a Carlina acaulis Yoghourt, it is characterised in that: including:
1) taking the Carlina acaulis stem and leaf powder that particle diameter is 10~30 mesh, add 55~70 DEG C of water of 7~10 times amount, 600~1000W is micro- Ripple extraction 80~120s, concentrated extracting solution, obtain Carlina acaulis extractum;Carry out macroporous adsorbent resin subsequently isolated and purified, Carlina acaulis Extractum according to 1~4BV/h volume flow loading, 45~55% ethanol elution of 2~5BV, elution speed is 1~4BV/h, To eluent be condensed into the clear paste of relative density 1.08~1.32 at 50 DEG C, lyophilization 48~75h, obtain Carlina acaulis lyophilizing Powder, wherein the content of cynarin is 4~6%;
2) according to the ratio that mass ratio is 8~12:100 of whole milk powder Yu water, whole milk powder is dissolved in 30~45 DEG C of warm water, Addition 3~12% sucrose, mixing, filter, sterilizing 12~20min at 85~100 DEG C, after sterilizing, rapidly milk is cooled to 30 ~42 DEG C;
3) by step 1) the Carlina acaulis lyophilized powder that obtains and step 2) milk that obtains is according to the ratio of mass volume ratio 1:45~200 Example mixes, and filters, and filtrate is heated to 75~90 DEG C, homogeneous micronization under 18~25MPa;
4) by step 3) pH value of mixed liquor after the homogenizing that obtains is adjusted to 5.8~6.5, then through 122~135 DEG C, 1~3s high temperature Instantaneous sterilizing, is cooled to the most rapidly 38~42 DEG C;By Lactobacillus bulgaricus and streptococcus thermophilus with mass ratio as 1:0.8~ After the ratio mixing of 1.2, according to inoculum concentration 1.5~9% inoculation, ferment at constant temperature 3~24h at 41~43 DEG C subsequently;
5) in step 4) tunning that obtains accesses 0.1~the stabilizer of 0.6%, at 3~6 DEG C, after-ripening 8~12h, to obtain final product Finished product.
Preparation method the most according to claim 1, it is characterised in that: described step 2) in, sucrose addition be 8~ 10%.
Preparation method the most according to claim 1, it is characterised in that: described step 4) in, inoculum concentration is 6~8%.
Preparation method the most according to claim 1, it is characterised in that: described step 5) in, the addition of stabilizer is 0.4 ~0.5%.
5. the Carlina acaulis Yoghourt that prepared by a preparation method according to any one of claim 1 to 4.
CN201610505951.3A 2016-06-30 2016-06-30 A kind of Carlina acaulis Yoghourt and preparation method thereof Pending CN106070632A (en)

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CN110250271A (en) * 2019-07-09 2019-09-20 中国农业大学 A kind of preparation method of sour milk beverage

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Application publication date: 20161109