CN107836521A - A kind of sophora bud Yoghourt and preparation method thereof - Google Patents

A kind of sophora bud Yoghourt and preparation method thereof Download PDF

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Publication number
CN107836521A
CN107836521A CN201711397727.8A CN201711397727A CN107836521A CN 107836521 A CN107836521 A CN 107836521A CN 201711397727 A CN201711397727 A CN 201711397727A CN 107836521 A CN107836521 A CN 107836521A
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sophora bud
yoghourt
group
sophora
bud
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CN107836521B (en
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黄昕颖
郑金贵
程祖锌
肖长春
许明
曹晓华
刘江洪
廖素凤
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Fujian Agriculture and Forestry University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C3/00Preservation of milk or milk preparations
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C2240/00Use or particular additives or ingredients
    • A23C2240/15Use of plant extracts, including purified and isolated derivatives thereof, as ingredient in dairy products

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of sophora bud Yoghourt and preparation method thereof, and it is to be made by sophora bud extraction concentrate, sucrose, bacterium powder, skimmed milk power and water for raw material.Sophora bud Yoghourt of the present invention is without any chemical addition agents such as stabilizers; and carry the distinctive delicate fragrance of the sophora bud; mouthfeel is pure and fresh, sour and sweet palatability, soft exquisiteness; outward appearance smooth uniform; and rich in bioactive ingredients such as rutin, Quercetins, there are the multiple functions such as reducing blood lipid, protection angiocarpy, liver protecting, anti-oxidant.

Description

A kind of sophora bud Yoghourt and preparation method thereof
Technical field
The invention belongs to food processing technology field, and in particular to one kind has reducing blood lipid, protection angiocarpy, liver protection shield Sophora bud Yoghourt of the function such as liver, anti-oxidant and preparation method thereof.
Background technology
The problem of modern society, is on the rise, and diet structure is unbalanced, operating pressure is big, lacks motion and improperly gives birth to Mode living etc., can cause dyslipidemia.The metabolism of liver and lipid is closely related, the synthesizing of cholesterol and protein, fat Synthesis oxidation of acid etc. is carried out all in liver.The internal long-term abnormal human body that can damage of blood lipid level is healthy, causes artery Atherosis, meanwhile, it will also destroy liver function, induced Diabetic, hypertension and a series of cardiovascular and cerebrovascular diseases etc..Meanwhile Harm of the dyslipidemia to human body is progressive, often without obvious symptom.Harm understanding deficiency of the people to dyslipidemia, is owed Preventive treatment knowledge is lacked, and does not give enough attention.
In the present age allegro life, convenient, health food has turned into the first choice of people.And have convenience, health concurrently The Yoghourt of feature, its demand also rise year by year.The Yoghourt for the addition raw material such as fruit juice or pulp more typical at present, also have few Amount adds the Yoghourt of some fortification materials, and toward in Yoghourt addition bioactive ingredients it is also more rare.Be probably because The mouthfeel of Yoghourt can be significantly influenceed for the bitter taste that some biological activity composition carries, and some biological activity composition is also It may be reacted with the composition in Yoghourt, it is counter to decline nutrition and influence digestion such as tannic acid and casein reaction caking.
《Fermented type sophora bud low sugar yoghurt technical study》(Tan Changzheng, 2006)Disclose a kind of fermented type sophora bud low sugar yoghurt Technique, but need addition such as gelatin, propylene glycol alginate stabilizer Yoghourt is kept stable;Nor it is rich in reed Fourth, Quercetin isoreactivity composition.《Sophora bud Production of Yogurt and the research of DPPH radical scavenging activities》(Guo Junhua, 2016)It is public A kind of sophora bud Production of Yogurt is opened, its sophora bud sour milk products prepared is coordinated in light green color, homogeneous, fragrance, mouthfeel Exquisiteness, but it is to be directly added into sophora bud powder, can not be effectively increased the active component of the sophora bud in Yoghourt, not rich in rutin, Mongolian oak Skin element isoreactivity composition.The present invention for target, will be enriched in rutin, Quercetin isoreactivity composition with " putting prevention first and combining prevention with control " Extract solution from pagodatree flower bud and skimmed milk power co-fermentation, not only mouthfeel is good for obtained sophora bud Yoghourt, and lipid-lowering effect is notable, also simultaneously With protection angiocarpy, liver protecting, improve the multiple efficacies such as human body oxidation resistance.In addition, rutin contained by sophora bud Yoghourt, Quercetin etc. has certain antibacterial effect, can delay the rear acidifying of Yoghourt, ensure the mouthfeel of Yoghourt, while extend Yoghourt Storage period.
The content of the invention
Object of the present invention is to provide a kind of good mouthfeel, and with reducing blood lipid, protection angiocarpy, liver protecting, Improve sophora bud Yoghourt of human body oxidation resistance and other effects and preparation method thereof.
To achieve the above object, the present invention adopts the following technical scheme that:
A kind of sophora bud Yoghourt, its raw materials used percentage are:Sophora bud extraction concentrate 12.5 ~ 25%, sucrose 6%, bacterium Powder 2%, skimmed milk power 5.56 ~ 9.72%, water 44.44 ~ 66.67%, the percetage by weight sum of each raw material is 100%.
The preparation method of the sophora bud Yoghourt comprises the following steps:
(1)70 DEG C of water is added in skimmed milk power, is sufficiently stirred after dissolving it, used filter mesh sieve is removed caused by stirring Foam, in order to avoid excessive foam influences the quality of Yoghourt, then it is sterilized after skim milk;
(2)In an aseptic environment, bacterium powder is added in the skim milk of sterilizing, stirred;
(3)Treat step(2)Milk be cooled to 40-45 DEG C after, sophora bud extraction concentrate and sucrose is added, after stirring Ferment 8 h in 42 DEG C, then is quickly cooled to 4 DEG C, that is, the sophora bud Yoghourt is made.
Wherein, the bacterium powder is by streptococcus thermophilus, lactobacillus delbruockii subspecies bulgaricus, bifidobacterium lactis, not tally bifid It is arbitrarily two or more in bacillus, lactobacillus acidophilus to mix.
The preparation method of the sophora bud extraction concentrate comprises the following steps:
1)80 mesh sieves are crossed after the dry sophora bud is crushed, by mass volume ratio 1:10 g/mL add water, sonicated 5 ~ 10 min Afterwards, it is transferred to the min of microwave treatment 5 ~ 8(The W of microwave power 320), depressurize filter while hot;
2)Toward step 1)Filter residue in add with the water of first time same volume, repeat the min of microwave treatment 5 ~ 8(Microwave power 320 W), depressurize filter while hot;
3)By step 2)The filtrate for filtering gained three times once, is then merged, substance is concentrated into 43 DEG C ~ 55 DEG C by reprocessing Long-pending 1/3, is produced.
Rutin content is up to 122.14 ~ 242.23mg/100mL in gained sophora bud Yoghourt, quercetin content up to 19.61 ~ 37.41mg/100mL。
Yoghourt produced by the present invention carries the distinctive delicate fragrance of the sophora bud, and mouthfeel is pure and fresh, sour and sweet palatability, soft exquisiteness, and outward appearance is put down It is sliding uniform, and rich in bioactive ingredients such as rutin, Quercetins, have the healthcare function of both Yoghourt, the sophora bud concurrently, there is drop blood Fat, protect angiocarpy, liver protecting, compared with multiple functions such as strong anti-oxidations;Simultaneously as rich in rutin, Quercetin isoreactivity Composition, certain antibacterial effect is made it have, the rear acidifying of Yoghourt can be delayed, advantageously ensure that the mouthfeel of Yoghourt, extend acid The storage period of milk.
The mode that the present invention is combined using ultrasound and microwave treatment is extracted, i.e., first by mass volume ratio 1:10 g/ The sophora bud aqueous solution that mL is prepared is ultrasonically treated, and because the ratio of the sophora bud and water is appropriate, minute bubbles in water can be utilized to occur Resonance, make sophora bud cell membrane, membranolysis, promote active component dissolution therein;Again through the micro- of 320 W power after ultrasound Ripple processing, can make sophora bud cell wall perforation, further promote the dissolution of active component in the sophora bud.Wherein, if microwave power mistake Height, it can make to produce solidification inside the sophora bud, hinder the dissolution of active component, reduce recovery rate;And if microwave power is too low, take It is long, and the dissolution rate of active component is also low.The present invention is combined using 5 ~ 10 min, the min of microwave treatment 5 ~ 8 is ultrasonically treated, not only The efficient dissolution of the active component in the sophora bud can be made, and temperature will not be made significantly raised, so as to be effectively retained the sophora bud Active component.
The present invention using lower temperature be concentrated by evaporation, the decomposition of active component can be reduced, largely retentive activity into Point.After measured, rutin content is up to 122.14 ~ 242.23mg/100mL in sophora bud Yoghourt of the present invention, and quercetin content is up to 19.61 ~ 37.41mg/100mL, and rutin, Quercetin can effectively delay the rear acidifying of Yoghourt in this content range, ensure The mouthfeel of Yoghourt, and extend the storage period of Yoghourt.
The present invention is prepared using sophora bud extraction concentrate, is not only made active component increase in Yoghourt, is also caused Yoghourt Stabilizer, which need not be added, can just keep smooth glossy outward appearance, avoid the precipitation directly easily caused using the sophora bud or sophora bud powder And lamination.
Tests prove that sophora bud Yoghourt of the present invention has reducing blood lipid, protection angiocarpy, liver protecting, raising human body antioxygen Change ability etc. acts on.
Embodiment
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention Technical scheme is described further, but the present invention is not limited only to this.
The preparation of the sophora bud Yoghourt of embodiment 1
The sophora bud sour milk products of three sections of various concentrations are prepared by the raw material proportioning of table 1.
The various concentrations sophora bud Yoghourt of table 1
Preparation method comprises the following steps:
(1)Take the dry sophora bud some, 80 mesh sieves are crossed after crushing, by mass volume ratio 1:10(g/mL)Addition water, sonicated 5 ~ After 10 min, microwave is transferred to(The W of power 320)5 ~ 8 min are handled, decompression while hot filters;Then toward addition in filter residue and first time The water of same volume, microwave(The W of power 320)5 ~ 8 min are handled, decompression while hot filters;Again toward addition and first time phase in filter residue The water of same volume, microwave(The W of power 320)5 ~ 8 min are handled, decompression while hot filters;The filtrate for filtering gained three times is merged, In Rotary Evaporators, 43 DEG C ~ 55 DEG C are concentrated into the 1/3 of original volume, obtain sophora bud extraction concentrate, take out, standby;
(2)Matched by table 1, extracting degreasing milk powder, add 70 DEG C of water wherein, after being sufficiently stirred dissolving, used filter mesh sieve removes Foam caused by stirring, skim milk is obtained after sterilizing;In aseptic operating platform, bacterium powder is taken, is added to sterilized skim milk In, stir;Treat that milk is cooled to 42 DEG C or so, add sophora bud extraction concentrate and sucrose, stir after 42 DEG C 8 h of fermentation, then are quickly cooled to 4 DEG C, that is, sophora bud Yoghourt are made.
The sophora bud Yoghourt sensory evaluation of embodiment 2
10 syndics are invited, sensory evaluation are carried out to the sophora bud Yoghourt of three sections of various concentrations in embodiment 1, and calculate the flat of each group Equal score.Standards of grading are:Poor 0 ~ 2 point, poor 2.1 ~ 4 points, medium 4.1 ~ 6 points, good 6.1 ~ 8 points, excellent 8.1 ~ 10 points.Scoring knot Fruit is shown in Table 2.
The Analyses Methods for Sensory Evaluation Results of the various concentrations sophora bud Yoghourt of table 2
Shown by the result of table 2, product 1, the sophora bud Yoghourt color and luster of product 2 be homogeneous, it is not stratified, give off a strong fragrance, be sticky, mouthfeel is relaxed It is suitable, entrance is pure and fresh oiliness;The curdled milk of product 3 is bad, there is yellow transparent liquid precipitation, and lamination occurs, and newborn taste is slightly blanked, Entrance slight bitter, mouthfeel is slightly puckery, and this is probably to inhibit zymogenic activity due to adding excessive sophora bud extraction concentrate.Therefore the sophora bud The addition for extracting concentrate is more suitable in the range of 12.5% ~ 25%.
The sophora bud Yoghourt Active Components of embodiment 3
To the sophora bud Yoghourt progress determination of bioactive constituent of product 1, product 2 in embodiment 1,3 are the results are shown in Table.
The content of active component in the various concentrations sophora bud Yoghourt of table 3
From table 3 it can be seen that add Yoghourt prepared by sophora bud extraction concentrate, its rutin content may be up to 122.14 ± 4.61 ~ 242.23±8.31mg/100mL;Quercetin may be up to 19.61 ± 0.67 ~ 37.41 ± 0.59mg/100mL.
The sophora bud yoghurt function of embodiment 4 is analyzed
The product 2 best to the sensory evaluation prepared by embodiment 1, active component is higher carries out functional analysis.
If normal group(CK), hyperlipidemia model group, 50 healthy SD male rats are grouped at random by body weight, normal group 10 Only, hyperlipidemia model group 40.Normal group feeding normal rats basal feed, hyperlipidemia model group feeding high lipid food, continuously feeds 4 In week, determine all rat limosis blood lipid levels.As a result show, hyperlipidemia model group fasting blood lipid level and normal group significant difference, Show modeling success.
Then hyperlipidemia model group is randomly divided into model group, medicine group, sophora bud concentrate group, sophora bud Yoghourt group four by body weight Group, every group 10.Normal group continues feeding basal feed;Model group feeding high lipid food;Medicine group feeding high lipid food, and often Day presses the amount gavage Simvastatin suspension of 1 mL/100g rat body weights once(Simvastatin suspension concentration is 0.2 mg/ mL);Sophora bud concentrate group feeding high lipid food, and the amount gavage sophora bud concentrate of 1.25 mL/100g rat body weights is pressed daily; Sophora bud Yoghourt group feeding high lipid food, and give sophora bud Yoghourt by the amount of 5 mL/100g rat body weights daily and supply its free diet. Continue 8 weeks, in 8 evening at weekend fasting(It can't help water), m seq weighs pneumoretroperitoneum injecting anesthetic, takes corresponding histoorgan to carry out Chemical examination.Each group rat blood serum, liver lipids are horizontal and the Indexs measure such as liver function, anti-oxidant, cardiovascular the results are shown in Table 4-table 9:
The rat body weight of table 4, fat body when liver index
As shown in Table 4, in experiment end week, sophora bud Yoghourt group rat body weight and normal group are without significant difference, all significantly lower than model group Rat and difference is extremely notable(P< 0.01), illustrate that sophora bud Yoghourt has certain effect of weight reducing.The sophora bud Yoghourt group rats'liver weight with Liver index is with normal group without significant difference, and all less than model group and medicine group, and difference is extremely notable(P< 0.01);Model group, The liver weight and liver index and normal group difference of medicine group rat are little, illustrate long term high-fat diet or take lipid-lowering medicine for a long time Thing all has certain detrimental effect to rat liver.Sophora bud concentrate group rats'liver weight with liver index also below model group and Medicine group, its significant difference(P< 0.05).Illustrate that feeding sophora bud concentrate and sophora bud Yoghourt have certain protection work(to liver Effect, and the positive effect of sophora bud Yoghourt is better than sophora bud concentrate.
The rat blood serum lipid content of table 5 and atherosclerosis index AI
As shown in Table 5, compared with model group:Sophora bud Yoghourt group rat blood serum TG is less than model group, and its difference is extremely notable(P< 0.01), and with normal group without significant difference;TC is less than model group, significant difference(P< 0.05);HDL is higher than model group, difference It is extremely notable(P< 0.01), and with normal group without significant difference;AI values are less than model group, and difference is extremely notable(P< 0.01), and just Chang Zuwu significant differences.
Compared with medicine group:Sophora bud Yoghourt group Serum TC and LDL are slightly above medicine with medicine group without significant difference, TG Group, significant difference(P< 0.05);AI is less than medicine group, and difference is extremely notable(P< 0.01), and HDL is higher than medicine group and difference pole Significantly(P< 0.01).Illustrate that sophora bud Yoghourt has significant effect for reducing fat, and its lipid-lowering effect cuts down him better than fat-reducing medicament is pungent Spit of fland.
Compared with sophora bud concentrate group, sophora bud Yoghourt group Serum TC, TG and LDL are therewith without significant difference, but HDL is high In sophora bud concentrate group, and difference is extremely notable(P< 0.01).Illustrate that compared with sophora bud concentrate, sophora bud Yoghourt can significantly improve Rat blood serum HDL.
The Hepaticlipid content of table 6(μmol/L)
As shown in Table 6, sophora bud Yoghourt group rat liver TC, TG is below model group and difference is extremely notable(P< 0.01).With medicine Group and sophora bud concentrate group compare:Sophora bud Yoghourt group rat liver TG is therewith without significant difference;TC is below medicine group and the sophora bud Concentrate group, and difference is extremely notable(P< 0.01).Illustrate that sophora bud Yoghourt is able to maintain that the horizontal stabilization of Hepaticlipid, and It reduces the effect of total cholesterol of liver and is better than lipid-lowering medicine Simvastatin and sophora bud concentrate.
The Liver Function index of table 7(U/L)
As shown in Table 7, model group, medicine group and the sophora bud concentrate group rat ALT, AST are all without significant difference(P> 0.05);Chinese scholartree Rice Yoghourt group rat ALT, AST is suitable with normal group, is below model group, and significant difference(0.01 <P< 0.05).Illustrate height Fat diet have to rat liver certain infringement, lipid-lowering medicine Simvastatin and sophora bud concentrate to the liver of Induced by High Fat Diet in Rats without Obvious protective function, and sophora bud Yoghourt has more excellent liver protecting effect.
The Antioxidation Ability of Rats of table 8
As shown in Table 8, medicine group and model group rats serum T-AOC and CAT indifference, illustrate lipid-lowering medicine Simvastatin to high fat Diet rat serum oxidation resistance is without influence.
The sophora bud Yoghourt group rat blood serum T-AOC and CAT are all higher than model group and difference is extremely notable(P< 0.01).Illustrate Chinese scholartree Rice Yoghourt can improve the oxidation resistance of Induced by High Fat Diet in Rats.
Compared with sophora bud concentrate group, the sophora bud Yoghourt group rat blood serum T-AOC and CAT are all higher than sophora bud concentrate group, and Difference is extremely notable therewith by CAT(P< 0.01).Illustrate that sophora bud Yoghourt is significantly better than Chinese scholartree to Induced by High Fat Diet in Rats serum antioxidant effect Rice concentrate.
The rat portions cardiovascular indicators of table 9
As shown in Table 9, the sophora bud Yoghourt group rat hs-CRP and Ang II is all less than model group and significant difference(P< 0.05);ET-1 And BNP is all less than model group and difference is extremely notable(P< 0.01), and it is little with normal group difference.Illustrate sophora bud Yoghourt to painstaking effort Pipe has good protective effect.
Sophora bud concentrate group rat ET-1 and BNP and sophora bud Yoghourt group difference are little, all extremely show less than model group and difference Write(P< 0.01), but there was no significant difference with model group by its hs-CRP and Ang II.Prove sophora bud Yoghourt to improving rat painstaking effort Effect in terms of pipe index is better than sophora bud concentrate.
In summary, in reducing blood lipid, reduction liver lipids level, liver protecting, raising serum oxidation resistance and improvement Cardiovascular indicators etc., sophora bud Yoghourt of the present invention can obtain better effects, and it is added without any chemistry such as stabilizer Agent, and the distinctive delicate fragrance of the sophora bud is carried, mouthfeel is pure and fresh, sour and sweet palatability, soft exquisiteness, outward appearance smooth uniform.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, without departing from the technical principles of the invention, some improvement and modification can also be made, these improvement and modification Also it should be regarded as protection scope of the present invention.

Claims (5)

1. a kind of sophora bud Yoghourt, it is characterised in that raw materials used percentage is:Sophora bud extraction concentrate 12.5 ~ 25%th, sucrose 6%, bacterium powder 2%, skimmed milk power 5.56 ~ 9.72%, water 44.44 ~ 66.67%, the percetage by weight sum of each raw material are 100%。
2. sophora bud Yoghourt according to claim 1, it is characterised in that in the sophora bud Yoghourt rutin content up to 122.14 ~ 242.23mg/100mL, quercetin content is up to 19.61 ~ 37.41mg/100mL.
3. sophora bud Yoghourt according to claim 1, it is characterised in that the preparation method of the sophora bud extraction concentrate includes Following steps:
1)80 mesh sieves are crossed after the dry sophora bud is crushed, by mass volume ratio 1:10 g/mL add water, sonicated 5 ~ 10 min Afterwards, the min of 320 W microwave treatments 5 ~ 8 is transferred to, decompression while hot filters;
2)Toward step 1)Filter residue in add with the water of first time same volume, the min of 320 W microwave treatments 5 ~ 8, depressurize while hot Filter;
3)By step 2)The filtrate for filtering gained three times once, is then merged, substance is concentrated into 43 DEG C ~ 55 DEG C by reprocessing Long-pending 1/3, is produced.
4. sophora bud Yoghourt according to claim 1, it is characterised in that the bacterium powder is by streptococcus thermophilus, Lactobacillus delbrueckii It is arbitrarily two or more in subspecies bulgaricus, bifidobacterium lactis, bifidobacterium bifidum, lactobacillus acidophilus to mix.
5. a kind of preparation method of sophora bud Yoghourt as claimed in claim 1, it is characterised in that comprise the following steps:
(1)70 DEG C of water is added in skimmed milk power, is sufficiently stirred after dissolving it, used filter mesh sieve is removed caused by stirring Foam, then it is sterilized after skim milk;
(2)In an aseptic environment, bacterium powder is added in the skim milk of sterilizing, stirred;
(3)Treat step(2)Milk be cooled to 40-45 DEG C after, sophora bud extraction concentrate and sucrose is added, after stirring Ferment 8 h in 42 DEG C, then is quickly cooled to 4 DEG C, that is, the sophora bud Yoghourt is made.
CN201711397727.8A 2017-12-21 2017-12-21 Sophora flower bud yoghourt and preparation method thereof Expired - Fee Related CN107836521B (en)

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CN114847475A (en) * 2022-07-05 2022-08-05 广东金骏康生物技术有限公司 Application of fermented sophora japonica rice flour for promoting growth of AKK (alkyl ketene dimer) bacteria
CN115336688A (en) * 2022-08-22 2022-11-15 江南大学 Method for preparing sophora flower bud beverage by hydrothermal treatment
CN115336688B (en) * 2022-08-22 2023-09-12 江南大学 Method for preparing pagodatree flower bud beverage through hydrothermal treatment

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