CN106069244A - A kind of method utilizing oil tea leaf nutrient to judge dose and application thereof - Google Patents

A kind of method utilizing oil tea leaf nutrient to judge dose and application thereof Download PDF

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CN106069244A
CN106069244A CN201610414503.2A CN201610414503A CN106069244A CN 106069244 A CN106069244 A CN 106069244A CN 201610414503 A CN201610414503 A CN 201610414503A CN 106069244 A CN106069244 A CN 106069244A
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oil tea
nutrient
dose
sample
leaf
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丁春邦
徐洲
刘静
周莉君
黄燕
廖进秋
袁明
杨瑞武
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Sichuan Agricultural University
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Sichuan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general

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  • Biodiversity & Conservation Biology (AREA)
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  • Cultivation Of Plants (AREA)
  • Hydroponics (AREA)

Abstract

The present invention relates to agricultural plantation technology field, a kind of method utilizing oil tea leaf nutrient to judge dose and application thereof, including: (1) samples selection;(2) sampling;(3) process;(4) element determination;(5) result judges.The rational application of fertilizer that the nutrient diagnosis method utilizing the present invention to propose is oil tea, formulate cultivation intensive management measure and scientific basis is provided, the disappearance of nutrient can not only be judged, and the equilibrium condition between each nutrient can be judged, thus reduce fertilising blindness, cost-effective, reduce the harmful effect to soil.Facts have proved the oil tea blade nutrient diagnosis method using the present invention to provide, be balanced the oil tea garden of fertilising, per mu yield increases by more than 33%, can effectively reduce large and small annual production difference.

Description

A kind of method utilizing oil tea leaf nutrient to judge dose and application thereof
Technical field
The present invention relates to agricultural plantation technology field, a kind of utilize oil tea leaf nutrient to judge dose Method and application thereof.
Background technology
Oil tea (Camellia oleifera Abel.) is Theaceae (Theaceae) Camellia (Camellia) evergreen filling Wood or dungarunga, be the distinctive woody oil tree species of China, big woody edible with Elaeis guineensis Jacq., Fructus oleae europaeae and Cortex cocois radicis the referred to as world four Oilseed plant, its mature seed can be used to refine Oleum Camelliae, is a kind of green, the edible health-care oil of ecological and high nutrition, has " east Olive oil " title, be the preferable edible oil of " three high " crowd, by FAO (Food and Agriculture Organization of the United Nation) (FAO) as emphasis promote strong Health table oil.Additionally, Camellia olelfera Abel [C.oleosa (Lour.) Rehd. is the most gorgeous, the florescence (the 9-11 month) is on the occasion of few adolescence, great ornamental value;Meanwhile, oil Tea or the extremely strong seeds of a contamination resistance, to the resistances such as sulfur dioxide, fluorine, chlorine, the great ecological value.For promoting oil Tea industry develops, and the State Administration of Forestry has formulated " whole nation camellia oleiferaindustry development plan (2009-2020) " for 2009, to the year two thousand twenty Plant 70,000,000 mu.At present, 250,000 mu of oil tea has been developed in Sichuan, to Regional Economic Development, promotes that peasant programme aspect is sent out Wave the most important effect.But current oil tea produces that the most generally to there is fruit-setting rate low, yield poorly and unstable, its reason degasification Outside time factor, the soil fertility that low level management causes is low and trunk infusion imbalance is one of the major reason of oil tea low yield.
Nutrition is growth and development of fruit tree, the basis of yield and quality raising, and nutrient is to affect fruit yield and quality One of key factor.Leaf nutrition elementary analysis can accurately reflect the Condition of abundance or deficiency that tree is recuperated point, leaf nutrient analysis It is one of important means of plant nutrient status evaluation with diagnosis.Sichuan Agricultural University is in 2012-2015, to Sichuan oil tea The tea oil tree that oil tea garden, 52, normal region is carried out recuperate point status investigation and lot of experiments accumulation data information on the basis of, foundation The variation amplitude of each constituent content of blade, feature, and consider that oil tea nutritional need characteristic determines oil tea Optimum range Optimum range.New diagnostic method is provided for oil tea nutrition diagnosis, rectification nutritional deficiency and balance fertilizing.
Summary of the invention
It is an object of the invention to cause the dystrophic generation of fruit tree growth fairly common for blindly applying fertilizer in producing, make Having become the improper underproduction of oil tea, change of production amplitude is big, also results in degradation problem under tree vigo(u)r, fruit quality, it is provided that a kind of utilization Oil tea leaf nutrient judges method and the application thereof of dose, may determine that oil tea is applied fertilizer by measuring oil tea Leaf nutrient contents The situation of amount.
For achieving the above object, the technical solution used in the present invention is: one utilizes oil tea leaf nutrient to judge dose Method, including following steps in sequence:
(1) samples selection: set the sample ground of 4 20 × 20m in field to be measured, according to S type route, each on each sample ground Select 5 strains as sampling tree;
(2) sampling: more than in the middle part of tree crown periphery, the current-year branch that growth selection is healthy and strong, respectively at east, south, west, north 4 directions gather mature leaf 20, seal and take back laboratory;
(3) processing: blade two sides wiped clean, remove petiole, complete under the conditions of weighing latter 100 DEG C 25min, then proceeds to 75 DEG C of constant temperature process 24h, again weigh, and load in sealing wide mouthed bottle standby after pulverizing 2mm sieve;
(4) element determination: claim leaf sample powder 0.3-0.4g to boil in disappearing and add concentrated sulphuric acid 5mL in pipe, places 10-12h, then adds double Oxygen water 2mL, is placed in 300 DEG C and disappears and boil on stove, and the most every 30min drips 10 hydrogen peroxide, drip 3-4 time boil to disappearing liquid limpid after again Heating 30min, after natural cooling, constant volume 50mL, transfers in plastic bottle standby;Offset the sample determination nitrogen after boiling, phosphorus, potassium, Use Kjeldahl nitrogen determination nitrogen, use molybdenum antimony resistance colorimetric method to measure phosphorus, use flame spectrophotometric determination potassium;
(5) result judges: testing result and the comparison of the normal nutrient requirement rate of oil tea.
Further, the normal nutrient requirement rate of described oil tea is: N be 10.55-12.15g/kg, P be 0.65-0.72g/ Kg, K be 3.85-5.75g/kg, P/N be 0.059-0.062, K/N be 0.365-0.472.
The present invention also provides for a kind of method of judgement dose utilizing oil tea leaf nutrient answering in oil tea dose With.
In the present invention, oil tea normal nutrient demand amount is that applicant lasts 4 years, to 52, oil tea normal region, Sichuan oil tea On the basis of the regression relation of the many annual productions in garden and leaf nitrogen and phosphorus, potassium content is studied, the conclusion drawn.
The method have the benefit that: the rational application of fertilizer that the nutrient diagnosis method utilizing the present invention to propose is oil tea, Formulate cultivation intensive management measure and scientific basis is provided, the disappearance of nutrient can not only be judged, and can judge between each nutrient Equilibrium condition, thus reduce fertilising blindness, cost-effective, reduce the harmful effect to soil.Facts have proved and use this The oil tea blade nutrient diagnosis method of bright offer, is balanced the oil tea garden of fertilising, and per mu yield increases by more than 33%, can effectively subtract Little large and small annual production difference.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, Obviously, described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based in the present invention Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all Belong to the scope of protection of the invention.
Embodiment 1
A kind of method utilizing oil tea leaf nutrient to judge dose, step includes:
(1) sample tree is chosen: June 21, sets the sample ground of 4 20 × 20m in certain field to be measured, according to " S " type route, Each sample ground respectively choose robust growth, tree vigo(u)r is good, tree crown is complete, without pest and disease damage sample tree 5 strain as sample set, choose altogether 20 strain sampling trees.
(2) field picking leaves method: more than in the middle part of tree crown periphery, the current-year branch that growth selection is healthy and strong, respectively at east, South, west, direction, 4, north gather mature leaf 20, pack with sealing bag and take back laboratory treatment.
(3) indoor leaf sample processes: gauze or towel with cleaning, by blade two sides wiped clean, remove petiole, with analysis Putting into after balance correct amount in baking oven, complete under the conditions of 100 DEG C 25min, is then placed in 75 DEG C of calorstats baking 24h, takes Going out and again weigh, sample comminution crosses 2mm sieve, loads in the wide mouthed bottle sealed, posts label, uses in case measuring to analyze.
(4) mensuration of foliar element: claim leaf sample powder 0.3g to boil in disappearing and add concentrated sulphuric acid 5mL in pipe, places 10h, then adds double Oxygen water 2mL, is placed in 300 DEG C and disappears and boil on stove, and the most every 30min drips 10 hydrogen peroxide, drip 3 times boil to disappearing liquid limpid after add again Hot 30min, after natural cooling, constant volume 50mL, transfers in plastic bottle standby;Offset the sample determination nitrogen after boiling, phosphorus, potassium, adopt Use Kjeldahl nitrogen determination nitrogen, use molybdenum antimony resistance colorimetric method to measure phosphorus, use flame spectrophotometric determination potassium.Measured result: N is 13.12g/kg, P be 0.69g/kg, K be 6.08g/kg;P/N is 0.053, and K/N is 0.463.
(5) the normal nutrient requirement rate of oil tea is: N be 10.55-12.15g/kg, P be 0.65-0.72g/kg, K be 3.85- 5.75g/kg, P/N be 0.059-0.062, K/N be 0.365-0.472.
(6) measurement result judges: according to step 4 measurement result, the nutrient diagnosis criterion that comparison step 5 provides, sentence The disappearance of disconnected sample strain nutrient and balance, this garden tea oil tree body nitrogen, potassium element excess, especially nitrogen element, and compare between element Example lack of proper care, therefore should increasing the p application rate, Shaoshi potash fertilizer, balance nitrogen.
Embodiment 2
A kind of method utilizing oil tea leaf nutrient to judge dose, step includes:
(1) sample tree is chosen: July 10, sets the sample ground of 4 20 × 20m in certain field to be measured, according to " S " type route, Each sample ground respectively choose robust growth, tree vigo(u)r is good, tree crown is complete, without pest and disease damage sample tree 5 strain as sample set, choose altogether 20 strain sampling trees.
(2) field picking leaves method: more than in the middle part of tree crown periphery, the current-year branch that growth selection is healthy and strong, respectively at east, South, west, direction, 4, north gather mature leaf 20, pack with sealing bag and take back laboratory treatment.
(3) indoor leaf sample processes: gauze or towel with cleaning, by blade two sides wiped clean, remove petiole, with analysis Putting into after balance correct amount in baking oven, complete under the conditions of 100 DEG C 25min, is then placed in 75 DEG C of calorstats baking 24h, takes Going out and again weigh, sample comminution crosses 2mm sieve, loads in the wide mouthed bottle sealed, posts label, uses in case measuring to analyze.
(4) mensuration of foliar element: claim leaf sample powder 0.35g to boil in disappearing and add concentrated sulphuric acid 5mL in pipe, places 10h, then adds double Oxygen water 2mL, is placed in 300 DEG C and disappears and boil on stove, and the most every 30min drips 10 hydrogen peroxide, drip 4 times boil to disappearing liquid limpid after add again Hot 30min, after natural cooling, constant volume 50mL, transfers in plastic bottle standby;Offset the sample determination nitrogen after boiling, phosphorus, potassium, adopt Use Kjeldahl nitrogen determination nitrogen, use molybdenum antimony resistance colorimetric method to measure phosphorus, use flame spectrophotometric determination potassium.Measured result: N is 10.95g/kg, P be 0.66g/kg, K be 4.15g/kg;P/N is 0.060, and K/N is 0.378.
(5) the normal nutrient requirement rate of oil tea is: N be 10.55-12.15g/kg, P be 0.65-0.72g/kg, K be 3.85- 5.75g/kg, P/N be 0.059-0.062, K/N be 0.365-0.472.
(6) measurement result judges: according to step 4 measurement result, the nutrient diagnosis criterion that comparison step 5 provides, sentence The disappearance of disconnected sample strain nutrient and balance, this garden each nutritive element balance of tea oil tree body.
Embodiment 3
A kind of method utilizing oil tea leaf nutrient to judge dose, step includes:
(1) sample tree is chosen: July 25, sets the sample ground of 4 20 × 20m in certain field to be measured, according to " S " type route, Each sample ground respectively choose robust growth, tree vigo(u)r is good, tree crown is complete, without pest and disease damage sample tree 5 strain as sample set, choose altogether 20 strain sampling trees.
(2) field picking leaves method: more than in the middle part of tree crown periphery, the current-year branch that growth selection is healthy and strong, respectively at east, South, west, direction, 4, north gather mature leaf 20, pack with sealing bag and take back laboratory treatment.
(3) indoor leaf sample processes: gauze or towel with cleaning, by blade two sides wiped clean, remove petiole, with analysis Putting into after balance correct amount in baking oven, complete under the conditions of 100 DEG C 25min, is then placed in 75 DEG C of calorstats baking 24h, takes Going out and again weigh, sample comminution crosses 2mm sieve, loads in the wide mouthed bottle sealed, posts label, uses in case measuring to analyze.
(4) mensuration of foliar element: claim leaf sample powder 0.40g to boil in disappearing and add concentrated sulphuric acid 5mL in pipe, places 10h, then adds double Oxygen water 2mL, is placed in 300 DEG C and disappears and boil on stove, and the most every 30min drips 10 hydrogen peroxide, drip 4 times boil to disappearing liquid limpid after add again Hot 30min, after natural cooling, constant volume 50ml, transfers in plastic bottle standby;Offset the sample determination nitrogen after boiling, phosphorus, potassium, adopt Use Kjeldahl nitrogen determination nitrogen, use molybdenum antimony resistance colorimetric method to measure phosphorus, use flame spectrophotometric determination potassium.Measured result: N is 13.15g/kg, P be 0.67g/kg, K be 6.05g/kg;P/N is 0.051, and K/N is 0.460.
(5) the normal nutrient requirement rate of oil tea is: N be 10.55-12.15g/kg, P be 0.65-0.72g/kg, K be 3.85- 5.75g/kg, P/N be 0.059-0.062, K/N be 0.365-0.472.
(6) measurement result judges: according to step 4 measurement result, the nutrient diagnosis criterion that comparison step 5 provides, sentence The disappearance of disconnected sample strain nutrient and balance, this garden tea oil tree body nitrogen excess, phosphorus deficiency, potassium element is appropriate, and between element, ratio is lost Adjust, therefore should increasing the p application rate, balance nitrogen.
Those skilled in the art of the present technique are appreciated that unless otherwise defined, and all terms used herein (include technology art Language and scientific terminology) have with the those of ordinary skill in art of the present invention be commonly understood by identical meaning.Also should Being understood by, those terms defined in such as general dictionary should be understood that the meaning having with the context of prior art The meaning that justice is consistent, and unless defined as here, will not explain by idealization or the most formal implication.
It should be noted last that: above example is only in order to illustrative not limiting technical scheme, although ginseng According to above-described embodiment, the present invention is described in detail, it will be apparent to an ordinarily skilled person in the art that: still can be to this Invention is modified or equivalent, and any modification or partial replacement without departing from the spirit and scope of the present invention, it is equal Should contain in the middle of scope of the presently claimed invention.

Claims (3)

1. one kind utilizes the method that oil tea leaf nutrient judges dose, it is characterised in that include following steps in sequence:
(1) samples selection: set the sample ground of 4 20 × 20m in field to be measured, according to S type route, respectively selects 5 on each sample ground Strain is as sampling tree;
(2) sampling: more than in the middle part of tree crown periphery, the current-year branch that growth selection is healthy and strong, respectively at 4, east, south, west, north Direction gathers mature leaf 20, seals and takes back laboratory;
(3) processing: blade two sides wiped clean, remove petiole, complete under the conditions of weighing latter 100 DEG C 25min, then proceeds to 75 DEG C Constant temperature processes 24h, again weighs, and loads in sealing wide mouthed bottle standby after pulverizing 2mm sieve;
(4) element determination: claim leaf sample powder 0.3-0.4g to boil in disappearing and add concentrated sulphuric acid 5mL in pipe, place 10-12h, then add hydrogen peroxide 2mL, is placed in 300 DEG C and disappears and boil on stove, and the most every 30min drips 10 hydrogen peroxide, drip 3-4 time and boils the limpid rear reheating of liquid to disappearing 30min, after natural cooling, constant volume 50mL, transfers in plastic bottle standby;Offset the sample determination nitrogen after boiling, phosphorus, potassium, use Kjeldahl nitrogen determination nitrogen, uses molybdenum antimony resistance colorimetric method to measure phosphorus, uses flame spectrophotometric determination potassium;
(5) result judges: testing result and the comparison of the normal nutrient requirement rate of oil tea.
Utilize the method that oil tea leaf nutrient judges dose the most according to claim 1, it is characterised in that described oil tea is just Often nutrient requirement rate is: N be 10.55-12.15g/kg, P be 0.65-0.72g/kg, K be that 3.85-5.75g/kg, P/N are 0.059-0.062, K/N are 0.365-0.472.
Oil tea leaf nutrient is utilized to judge the application in oil tea dose of the method for dose the most according to claim 1.
CN201610414503.2A 2016-06-13 2016-06-13 A kind of method utilizing oil tea leaf nutrient to judge dose and application thereof Pending CN106069244A (en)

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Cited By (5)

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Publication number Priority date Publication date Assignee Title
CN107114183A (en) * 2017-05-03 2017-09-01 滨州学院 A kind of soil management method for improving winter-jujube fruit quality
CN109168520A (en) * 2018-10-25 2019-01-11 江西省科学院生物资源研究所 A kind of research method of south jiangxi navel orange nutrient fertilization
CN110050555A (en) * 2019-04-23 2019-07-26 四川农业大学 A method of dose is judged using Citrus leaf nutrient
CN112119726A (en) * 2020-09-23 2020-12-25 四川省农业科学院茶叶研究所 Method for applying potash fertilizer to tea garden based on tree detection diagnosis and application of potash fertilizer
CN113219127A (en) * 2021-03-15 2021-08-06 中国林业科学研究院亚热带林业研究所 Camellia oleifera tree body nutrition diagnosis method based on leaf ion group synergistic change characteristics

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107114183A (en) * 2017-05-03 2017-09-01 滨州学院 A kind of soil management method for improving winter-jujube fruit quality
CN109168520A (en) * 2018-10-25 2019-01-11 江西省科学院生物资源研究所 A kind of research method of south jiangxi navel orange nutrient fertilization
CN109168520B (en) * 2018-10-25 2020-10-30 江西省科学院生物资源研究所 Research method for nutrient fertilization of Gannan navel orange
CN110050555A (en) * 2019-04-23 2019-07-26 四川农业大学 A method of dose is judged using Citrus leaf nutrient
CN112119726A (en) * 2020-09-23 2020-12-25 四川省农业科学院茶叶研究所 Method for applying potash fertilizer to tea garden based on tree detection diagnosis and application of potash fertilizer
CN112119726B (en) * 2020-09-23 2022-05-17 四川省农业科学院茶叶研究所 Method for applying potash fertilizer to tea garden based on tree detection diagnosis and application of potash fertilizer
CN113219127A (en) * 2021-03-15 2021-08-06 中国林业科学研究院亚热带林业研究所 Camellia oleifera tree body nutrition diagnosis method based on leaf ion group synergistic change characteristics

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Application publication date: 20161109