CN106053796B - A kind of detecting system and detection method chromatographed for PCT - Google Patents
A kind of detecting system and detection method chromatographed for PCT Download PDFInfo
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- CN106053796B CN106053796B CN201610561512.4A CN201610561512A CN106053796B CN 106053796 B CN106053796 B CN 106053796B CN 201610561512 A CN201610561512 A CN 201610561512A CN 106053796 B CN106053796 B CN 106053796B
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- valve
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- test paper
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Abstract
Include elevating mechanism, support, cleaning lid, test paper clamping plate, shell fragment, test bottle, discharging tube, feed tube, cleaning pipe, sample bottle and sewer pipe present invention is disclosed a kind of detecting system for PCT chromatographies.The advantage of the invention is that it is easy to use, installation operation step process is required to, difficulty of test is reduced, while by equipment automation control, improving the precision of test, it helps testing efficiency is improved.
Description
Technical field
The present invention relates to the system that the test strips that collaurum is made (PCT) carry out chromatography detection.
Background technology
Although carrying out clinical detection currently with collaurum very to have popularized, chromatographed currently with PCT
When realizing, being all to need staff manually operated, not only increase the uncontrollability in process of the test, being tested from there is influence
The hidden danger of accuracy.
The content of the invention
The technical problems to be solved by the invention are to realize that one kind convenient, fast and accurate can carry out chromatography intelligence inspection
The system of survey.
To achieve these goals, the technical solution adopted by the present invention is：A kind of detecting system chromatographed for PCT, puts down
Row support is made up of two parallel support bars, and parallel bracket level is fixed on elevating mechanism, wherein under a support bar
Surface is vertically provided with equidistantly fixed test paper clamping plate, and each test paper clamping plate is used to clamp test paper provided with least two
Shell fragment, another support bar lower surface is vertically provided with equidistantly fixed cleaning lid, and the elevating mechanism is fixed on translation
In mechanism；
Row's test bottle, quantity and cleaning lid, the number of test paper clamping plate of the test bottle are provided with below the parallel bracket
Amount is identical, and the fixed position of test bottle and position when cleaning is covered or test paper clamping plate is rotated to above test bottle are mutually corresponding；
Each test bottle is provided with the sample bottle that an installation site is higher than test bottle, and the sample bottle passes through feed liquor
Pipe connects test bottle, and the test bottle is connected with cleaning pipe, and the cleaning pipe connects water source, the test bottle bottom connection through pump
Have a discharging tube, the sample bottle bottom is connected with sewer pipe, the discharging tube is provided with tapping valve, the feed tube be provided with into
Liquid valve, the cleaning pipe is provided with purge valve, and the sewer pipe is provided with waist valve.
The translation mechanism includes track, installs sliding block in orbit, and the elevating mechanism is fixed on sliding block, described
The wheel and rack engagement being fixed with rack, the sliding block on stepper motor, the stepper motor output shaft is provided with track,
The track two ends are provided with the Hall sensor provided with measurement rotary torsion in gag lever post, the stepper motor.
The sample bottle be provided with scale, and sample bottle be provided with bottle cover bracket, the bottle cover bracket be provided with it is each
The corresponding bottle cap in sample bottle position, the bottle cover bracket is fixed on lift cylinder, and each bottle cap lower surface is equipped with out
Stomata, the venthole is connected through flexible pipe with hot gas source.
It is described to be arranged on for the detecting system that PCT is chromatographed in thermostatical storehouse, thermoregulation mechanism is provided with the thermostatical storehouse.
The side of the test paper Boards wall test paper for the PCT detecting systems chromatographed is fixed with image acquisition units,
Described image collecting unit output collection signal is to main control unit, the main control unit output image information to memory cell
And display unit.
The main control unit is provided with clock unit, and the main control unit outputs control signals to pump, tapping valve, feed liquor
Valve, purge valve, waist valve, elevating mechanism, stepper motor, hot gas source and lift cylinder, and receive the signal of Hall sensor.
Based on the detection method for the PCT detecting systems chromatographed：
Step 1, for the first time startup, elevating mechanism and lift cylinder are pushed, and cleaning lid blocks each test bottle, and bottle cap is blocked up
Firmly each sample bottle；
Step 2, open liquid feed valve, purge valve and waist valve, restart pump, clean time rating, close pump, turn off into
Liquid valve, purge valve and waist valve；
Step 3, open purge valve and tapping valve, restart pump, clean time rating, close pump, turn off purge valve and
Tapping valve；
Step 4, open liquid feed valve and tapping valve, start hot gas source, dry time rating, close hot gas source, turn off into
Liquid valve and tapping valve；
Step 5, elevating mechanism and lift cylinder rise, and wait enabled instruction next time；
Step 6, manually test paper is fixed on test paper clamping plate, quantitative test sample is injected in sample bottle；
Step 7, second of startup, test paper clamping plate is moved to above test bottle；
Step 8, elevating mechanism are pushed, and test paper clamping plate is put into test bottle；
Step 9, after the delay adjustmentses time, elevating mechanism rise, image acquisition units collection test paper image information；
Step 10, cleaning lid is moved to above test bottle, elevating mechanism and lift cylinder are pushed, and cleaning lid blocks each
Individual test bottle, bottle cap blocks each sample bottle；
Step 11, unlatching liquid feed valve, purge valve and waist valve, restart pump, clean time rating, close pump, turn off
Liquid feed valve, purge valve and waist valve；
Step 12, open purge valve and tapping valve, restart pump, clean time rating, close pump, turn off purge valve and
Tapping valve；
Step 13, open liquid feed valve and tapping valve, start hot gas source, dry time rating, close hot gas source, turn off into
Liquid valve and tapping valve；
Step 14, elevating mechanism and lift cylinder rise, and once test terminates.
The advantage of the invention is that it is easy to use, installation operation step process is required to, difficulty of test is reduced, led to simultaneously
Equipment automation control is crossed, the precision of test is improved, it helps testing efficiency is improved.
Brief description of the drawings
The mark in the content and figure of every width accompanying drawing expression in description of the invention is briefly described below：
Fig. 1 is the detecting system structural representation chromatographed for PCT；
Mark in above-mentioned figure is：1st, elevating mechanism；2nd, parallel bracket；3rd, cleaning lid；4th, test paper clamping plate；5th, shell fragment；
6th, test bottle；7th, discharging tube；8th, feed tube；9th, cleaning pipe；10th, sample bottle；11st, sewer pipe.
Embodiment
As shown in figure 1, including elevating mechanism 1, parallel bracket 2, cleaning lid 3, test paper folder for the detecting system that PCT is chromatographed
Plate 4, shell fragment 5, test bottle 6, discharging tube 7, feed tube 8, cleaning pipe 9, sample bottle 10 and sewer pipe 11.The inspection chromatographed for PCT
Examining system is arranged in thermostatical storehouse, and thermoregulation mechanism is provided with thermostatical storehouse, so can by the temperature in regulating thermostatic storehouse,
Make further control to experimental enviroment, improve the reliability and controllability of experiment.
System is provided with parallel bracket 2, and parallel bracket 2 is made up of two parallel support bars, and parallel bracket level, which is fixed, to be risen
On descending mechanism 1, wherein a support bar lower surface is vertically provided with equidistantly fixed test paper clamping plate 4, each test paper clamping plate 4
Shell fragment 5 provided with least two for clamping test paper, another support bar lower surface is vertically provided with equidistantly fixed cleaning
Lid 3, elevating mechanism 1 is fixed on translation mechanism；Clean position of the lid 3 with test paper clamping plate 4 on two support bars identical, pass through
Translation mechanism is adjusted, and the support bar for cleaning lid 3 or test paper clamping plate 4 by being fixed with that can be selective is moved to the top of test bottle 6.
Translation mechanism includes track, installs sliding block in orbit, and elevating mechanism 1 is fixed on sliding block, is provided with track
The wheel and rack engagement on stepper motor, stepper motor output shaft is fixed with rack, sliding block, track two ends are provided with spacing
Hall sensor provided with measurement rotary torsion in bar, stepper motor.Gag lever post limits the sliding scale of sliding block, recycles Hall
Sensor can avoid overexercise, and both can accurately control the translation distance of parallel bracket 2 at cooperation.
Wherein one support bar lower surface is provided with equidistantly fixed test paper clamping plate 4, another support bar vertically
Surface is vertically provided with equidistantly fixed cleaning lid 3, and each test paper clamping plate 4 is used to clamp test paper provided with least two
Shell fragment 5.
The lower section of support bar is provided with row's test bottle 6, test bottle 6 with clean lid 3, test paper clamping plate 4 be it is matching used, because
The quantity of this test bottle 6 and cleaning lid 3, and the quantity of test bottle 6 are identical, supports every time with the quantity of test paper clamping plate 4
After bar position adjustment, position is mutual when the fixed position of test bottle 6 moves to 6 top of test bottle with cleaning lid 3 or test paper clamping plate 4
Correspondence, i.e., each cleaning lid 3 can fill in the bottleneck to a test bottle 6, and each test paper clamping plate 4 can be put into a test bottle
In 6 bottle.
Each test bottle 6 is provided with the sample bottle 10 that an installation site is higher than test bottle 6, i.e. liquid in sample bottle 10
Can be by the way that in gravity flowing to test bottle 6, sample bottle 10 connect test bottle 6 by feed tube 8, test bottle 6 is connected with cleaning
Pipe 9, cleaning pipe 9 connects water source through pump, and the bottom of test bottle 6 is connected with discharging tube 7, and the bottom of sample bottle 10 is connected with sewer pipe 11,
Discharging tube 7 is provided with tapping valve, and feed tube 8 is provided with liquid feed valve, and cleaning pipe 9 is provided with purge valve, and sewer pipe 11 is provided with useless
Liquid valve.
Sample bottle 10 is provided with scale, and convenient use person accurately imports measurement liquid, and sample bottle 10 is provided with bottle cap
Support, quantity of the bottle cover bracket provided with bottle cap corresponding with each position of sample bottle 10, i.e. bottle cap and sample bottle 10, position are equal
Identical and correspondingly, bottle cover bracket is fixed on lift cylinder, and each bottle cap lower surface is equipped with venthole, venthole through flexible pipe with
Hot gas source is connected, and can dry ponding by hot gas source, it is to avoid have influence on result of the test.Sample bottle 10 and test bottle 6 are used
Transparent vial body, convenient observation.
In order to improve the reliability of capping, bottle cap and the cleaning upper surface of lid 3 pass through spring and lift cylinder, elevating mechanism 1
Connection, lower surface is provided with rubber blanket, so that it is guaranteed that sealing during cleaning.
In order to coordinate whole system work, system is provided with main control unit, and main control unit is provided with clock unit, main
Control unit outputs control signals to pump, tapping valve, liquid feed valve, purge valve, waist valve, elevating mechanism 1, stepper motor, hot gas
Source and lift cylinder, and the signal of Hall sensor is received, the test paper clamping plate 4 for the PCT detecting systems chromatographed fixes test paper
Side be fixed with image acquisition units, image acquisition units output collection signal to main control unit, main control unit output
Image information so can accurately record the test paper state after test to memory cell and display unit.
Based on the above-mentioned control method for the PCT detecting systems chromatographed：
Step 1, for the first time startup, elevating mechanism 1 and lift cylinder are pushed, and cleaning lid 3 blocks each test bottle 6, bottle
Lid blocks each sample bottle 10；(being that cleaning lid 3 is located at the top of test bottle 6 after terminating every time)；
Step 2, open liquid feed valve, purge valve and waist valve, restart pump, clean time rating, close pump, turn off into
Liquid valve, purge valve and waist valve；
Step 3, open purge valve and tapping valve, restart pump, clean time rating, close pump, turn off purge valve and
Tapping valve；
So as to be cleaned to sample bottle 10 and test bottle 6, it is to avoid interference；
Step 4, open liquid feed valve and tapping valve, start hot gas source, dry time rating, close hot gas source, turn off into
Liquid valve and tapping valve；
Step 5, elevating mechanism 1 and lift cylinder rise, and wait enabled instruction next time；
Step 6, manually test paper is fixed on test paper clamping plate 4, the injection quantitative test sample in sample bottle 10；
Step 7, second of startup, the top of test bottle 6 is moved to by test paper clamping plate 4；
Step 8, elevating mechanism 1 are pushed, and test paper clamping plate 4 is put into test bottle 6；
Step 9, after the delay adjustmentses time, elevating mechanism 1 rises, image acquisition units collection test paper image information；
Step 10, cleaning lid 3 is moved to the top of test bottle 6, elevating mechanism 1 and lift cylinder are pushed, and cleaning lid 3 is blocked
Each test bottle 6, bottle cap blocks each sample bottle 10；
Step 11, unlatching liquid feed valve, purge valve and waist valve, restart pump, clean time rating, close pump, turn off
Liquid feed valve, purge valve and waist valve；
Step 12, open purge valve and tapping valve, restart pump, clean time rating, close pump, turn off purge valve and
Tapping valve；
So as to be cleaned to sample bottle 10 and test bottle 6, it is to avoid interference, it is to avoid interfered to testing next time；
Step 13, open liquid feed valve and tapping valve, start hot gas source, dry time rating, close hot gas source, turn off into
Liquid valve and tapping valve；
Step 14, elevating mechanism 1 and lift cylinder rise, and once test terminates.
All operations of intelligent automaticization, specification testing process, accurate test result, and facilitate staff
Use, multigroup sample can be tested under same environment simultaneously, it is convenient to compare and record.
The present invention is exemplarily described above in conjunction with accompanying drawing, it is clear that the present invention is implemented not by aforesaid way
Limitation, as long as the improvement of the various unsubstantialities of inventive concept and technical scheme of the present invention progress is employed, or without changing
Enter and the design of the present invention and technical scheme are directly applied into other occasions, within protection scope of the present invention.
Claims (6)
1. a kind of detecting system chromatographed for PCT, it is characterised in that：
Parallel bracket is made up of two parallel support bars, and parallel bracket level is fixed on elevating mechanism, wherein a branch
Strut lower surface is vertically provided with equidistantly fixed test paper clamping plate, and each test paper clamping plate is used to clamp provided with least two
The shell fragment of test paper, another support bar lower surface is vertically provided with equidistantly fixed cleaning lid, and the elevating mechanism is fixed
On translation mechanism；
Row's test bottle, the quantity and the quantity phase of cleaning lid, test paper clamping plate of the test bottle are provided with below the parallel bracket
Together, and position is mutually corresponding when the fixed position and cleaning lid or test paper clamping plate of test bottle is moved to above test bottle；
Each test bottle is provided with the sample bottle that an installation site is higher than test bottle, and the sample bottle is connected by feed tube
Logical test bottle, the test bottle is connected with cleaning pipe, and the cleaning pipe connects water source through pump, and the test bottle bottom is connected with row
Liquid pipe, the sample bottle bottom is connected with sewer pipe, and the discharging tube is provided with tapping valve, and the feed tube is provided with feed liquor
Valve, the cleaning pipe is provided with purge valve, and the sewer pipe is provided with waist valve;
The detection method for the PCT detecting systems chromatographed：
Step 1, for the first time startup, elevating mechanism and lift cylinder are pushed, and cleaning lid blocks each test bottle, and bottle cap is blocked often
One sample bottle；
Step 2, unlatching liquid feed valve, purge valve and waist valve, restart pump, clean time rating, close pump, turn off feed liquor
Valve, purge valve and waist valve；
Step 3, unlatching purge valve and tapping valve, restart pump, clean time rating, close pump, turn off purge valve and discharge opeing
Valve；
Step 4, unlatching liquid feed valve and tapping valve, start hot gas source, dry time rating, close hot gas source, turn off liquid feed valve
And tapping valve；
Step 5, elevating mechanism and lift cylinder rise, and wait enabled instruction next time；
Step 6, manually test paper is fixed on test paper clamping plate, quantitative test sample is injected in sample bottle；
Step 7, second of startup, test paper clamping plate is moved to above test bottle；
Step 8, elevating mechanism are pushed, and test paper clamping plate is put into test bottle；
Step 9, after the delay adjustmentses time, elevating mechanism rise, image acquisition units collection test paper image information；
Step 10, cleaning lid is moved to above test bottle, elevating mechanism and lift cylinder are pushed, and cleaning lid blocks each survey
Trial jar, bottle cap blocks each sample bottle；
Step 11, unlatching liquid feed valve, purge valve and waist valve, restart pump, clean time rating, close pump, turn off feed liquor
Valve, purge valve and waist valve；
Step 12, unlatching purge valve and tapping valve, restart pump, clean time rating, close pump, turn off purge valve and discharge opeing
Valve；
Step 13, unlatching liquid feed valve and tapping valve, start hot gas source, dry time rating, close hot gas source, turn off liquid feed valve
And tapping valve；
Step 14, elevating mechanism and lift cylinder rise, and once test terminates.
2. the detecting system according to claim 1 chromatographed for PCT, it is characterised in that：The translation mechanism includes rail
Road, installation sliding block in orbit, the elevating mechanism are fixed on sliding block, are provided with the track on rack, the sliding block
The wheel and rack engagement on stepper motor, the stepper motor output shaft is fixed with, the track two ends are provided with gag lever post, institute
State the Hall sensor provided with measurement rotary torsion in stepper motor.
3. the detecting system according to claim 1 or 2 chromatographed for PCT, it is characterised in that：The sample bottle is provided with
Scale, and sample bottle is provided with bottle cover bracket, the bottle cover bracket is provided with bottle cap corresponding with each sample bottle position, described
Bottle cover bracket is fixed on lift cylinder, and each bottle cap lower surface is equipped with venthole, and the venthole is through flexible pipe and heat
Source of the gas is connected.
4. the detecting system according to claim 3 chromatographed for PCT, it is characterised in that：The inspection chromatographed for PCT
Examining system is arranged in thermostatical storehouse, and thermoregulation mechanism is provided with the thermostatical storehouse.
5. the detecting system according to claim 4 chromatographed for PCT, it is characterised in that：The inspection chromatographed for PCT
The side of the test paper Boards wall test paper of examining system is fixed with image acquisition units, described image collecting unit output collection signal
To main control unit, the main control unit output image information to memory cell and display unit.
6. the detecting system according to claim 5 chromatographed for PCT, it is characterised in that：The main control unit is provided with
Clock unit, the main control unit output control signals to pump, tapping valve, liquid feed valve, purge valve, waist valve, elevating mechanism,
Stepper motor, hot gas source and lift cylinder, and receive the signal of Hall sensor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610561512.4A CN106053796B (en) | 2016-07-15 | 2016-07-15 | A kind of detecting system and detection method chromatographed for PCT |
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| CN201610561512.4A CN106053796B (en) | 2016-07-15 | 2016-07-15 | A kind of detecting system and detection method chromatographed for PCT |
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| CN106053796B true CN106053796B (en) | 2017-09-29 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1121370A (en) * | 1993-03-17 | 1996-04-24 | 阿克佐诺贝尔公司 | Apparatus for the detection of a specifically reacting substance |
| EP0693688A2 (en) * | 1994-07-19 | 1996-01-24 | Roche Diagnostics GmbH | Test-strip reader having test-strip transporter |
| JPH10316291A (en) * | 1997-05-19 | 1998-12-02 | Kdk Corp | Short member carrying device |
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Effective date of registration: 20171127 Address after: 241000 Anhui Province, Wuhu city Jinghu District long Fang Culture Park (big long Fang No. 77) B03 Building Room 303 Patentee after: Anhui National Science Biotechnology Co., Ltd. Address before: West Road in Shushan District of Hefei City, Anhui Province, No. 32 230000 Co-patentee before: Li Yufeng Patentee before: Tao Shaoqiang Co-patentee before: Zhang Shiwei Co-patentee before: Zhang Bin Co-patentee before: Wang Xiaoyang Co-patentee before: Yang Yanqi |