CN1060518C - Method for producing dictyophora indusiata nutritive mycelium using bamboo fungus submerged culture fermentation technology - Google Patents
Method for producing dictyophora indusiata nutritive mycelium using bamboo fungus submerged culture fermentation technology Download PDFInfo
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- CN1060518C CN1060518C CN96115299A CN96115299A CN1060518C CN 1060518 C CN1060518 C CN 1060518C CN 96115299 A CN96115299 A CN 96115299A CN 96115299 A CN96115299 A CN 96115299A CN 1060518 C CN1060518 C CN 1060518C
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- dictyophora indusiata
- mycelium
- dictyophora phalloidea
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Abstract
The present invention relates to a method for producing a dictyophora indusiata nutritive mycelium by using a submerged fermentation technology of dictyophora indusiata. In the method, the dictyophora indusiata nutritive mycelium is prepared from dictyophora indusiata strains, and produced by adopting the submerged fermentation technology and utilizing a mixed enzyme strain of two or multiple kinds of lysozymes, proteinases, cellulases and hemicellulases for the enzymatic hydrolysis of the dictyophora indusiata strains; a culture medium adopted in shaking culture contains mealy potato, peptones, glucose, KH2PO4, MgSO4 and NaCl, and culture liquor adopted in submerged fermentation contains a soybean cake powder, peptones, a yeast powder, corn steep liquor, sucrose, a corn powder, KH2PO4, MgSO4 and NaCl; dictyophora indusiata components prepared by the technology are easy to absorb by human bodies, and can be made into various foods and beverages which contain the dictyophora indusiata components.
Description
The method of dictyophora phalloidea vegetative mycelium
The present invention relates to the biochemical engineering field, the production method of a kind of dictyophora phalloidea bacterium is provided.The mycelium that specifically utilizes the dictyophora phalloidea submerged fermentation to obtain is raw material, through the complex enzyme hydrolysis effect its available nutrient and active substance easily are absorbed by the body, except that the mycelium water soluble component, also comprise total free aminoacids etc. in non-molten water section and the nutrient solution in this dictyophora phalloidea powder.
Dictyophora phalloidea is a kind of famous and precious edible and medicinal fungi, its health Dictyophare phalloidea drink and flavouring production are raw material with its sporophore all, cost is higher, mainly be the methods such as immersion, poach or alcohol extracting that adopt sporophore, add some medicinal herb componentses and make the dictyophora phalloidea seasonings among being mixed with drink, the health promoting wine that plurality of raw materials forms or joining seasonings.Also have report with perfume (or spice) eat, needle mushroom and dictyophora phalloidea solid fermentation mycelium, adding the blue and malt meal of strand bone is raw material, mixed extraction after squeezing, filtration make, for a kind of water is carried complex liquid.Yet three kinds of mycelium compositions in this method solid medium (based on cotton seed hull) are difficult to abundant acquisition, mix the aqueous extract that cotton seed hull and wheat bran etc. are arranged in the water extract.So far find to utilize the submerged fermentation technology to obtain mycelium as yet and be raw material, produce the report of various health Dictyophare phalloidea product with the mycelia.Purpose of the present invention provides a kind of biochemical method of producing the dictyophora phalloidea composition.
The method of production of the present invention dictyophora phalloidea powder is to utilize the submerged fermentation technology to produce the dictyophora phalloidea vegetative mycelium, in conjunction with the complex enzyme hydrolysis effect, forms that pure dictyophora phalloidea becomes to be grouped into and easily by full nutrition dictyophora phalloidea powder that human body absorbed.Technological process specifically of the present invention is:
1. the dictyophora phalloidea bacterial strain is cultivated in submerged fermentation:
Get the dictyophora phalloidea inoculation in culturing bottle, carry out the shaking table shaking culture in medium liquid, the consisting of of substratum: potato soaks juice 10～20%M/V, peptone 0.1～1%, glucose 1～10%, KH
2PO
40.01～0.1%, MgSO
40.01～0.1%, NaCl 0.005～0.1%, all the other are water.Shake-flask culture carried out 1～10 hour with 30～80rmp/ component velocity under 20～30 ℃.And carried out flask culture 1～10 with same medium again with this liquid spawn.
Bacterial classification inoculation after the above-mentioned cultivation is carried out fermentation culture in fermentor tank, its nutrient solution is a soybean cake powder 5～15%, peptone 0.1～1%, yeast powder 0.01～0.5%, corn steep liquor 0.01～0.5%, sucrose 1～5%, Semen Maydis powder 1～5%, KH
2PO
40.01～0.5%, MgSO
40.01～0.1%, NaCl 0.05～0.1%.Stirring velocity is 50～150rmp/ branch, cultivates that centrifugation goes out thalline after 1～10 day.
2. complex enzyme degradation dictyophora phalloidea bacterium:
With the mycelium of collecting, move on in the complex enzyme hydrolysis liquid in 35～50 ℃ of following oscillatory reactions 1～10 hour, finish enzymolysis process, prozyme can be used N,O-Diacetylmuramidase, two or more mixed enzyme bacterium in proteolytic enzyme, cellulase or the hemicellulase.The rare MgSO of routine techniques is adopted in the allotment of complex enzyme hydrolysis liquid
4It is formulated that solution adds the enzyme bacterium, and the content of enzyme bacterium is 1～8% of enzymolysis solution.
Enzymolysis mixed solution behind enzymolysis routinely technology concentrate or directly make the biological products that contain dictyophora phalloidea.
Give further instruction below by example to technology of the present invention.
Example 1
(potato soaks juice 20%M/V, peptone 0.2%, glucose 2%, KH in 250ml triangular flask liquid nutrient medium with inclined-plane dictyophora phalloidea inoculation
2PO
40.05%, MgSO
40.05%, NaCl 0.01%) 100ml, after treating that mycelia is launched growth, shaking table shaking culture (80rmp/min), and after liquid spawn carries out triangular flask liquid culture (20 ℃ of 144hr) again, be inoculated in 5 liter air lift type automatic fermenter liquid medium (soybean cake powder 10%, peptone 0.3%, yeast powder 0.2%, corn steep liquor 0.1%, sucrose 2%, Semen Maydis powder 1.5%, KH
2PO
40.1%, MgSO
40.05%, NaCl 0.01%), air flow 0.8～1.0V/V/M, stirring velocity 120rmp/min cultivates and centrifugal after seven days thalline is moved into complex enzyme hydrolysis liquid (0.6M MgSO
4Preparation cellulase 1%, hemicellulase 1%, N,O-Diacetylmuramidase 0.5%, proteolytic enzyme 1%), 40 ℃ of oscillatory reactions concentrated together with the bacteriological filtration supernatant liquor after 5 hours, and spray-dried afterwards, sterilization promptly gets full nutrition dictyophora phalloidea powder.
By above-mentioned example, but the characteristics of the technology of the present invention are to adopt the submerged fermentation technology of suitability for industrialized production, produce the dictyophora phalloidea vegetative mycelium and also utilize the complex enzyme degradation mycelium protein, discharge effective constituent in the cell, make easily the pure dictyophora phalloidea powder by human body absorbed.
Claims (1)
1. method of utilizing dictyophora phalloidea submerged fermentation technology to produce the dictyophora phalloidea vegetative mycelium, it is characterized in that getting the dictyophora phalloidea inoculation in culturing bottle, in medium liquid, carry out the shaking table shaking culture, consisting of of substratum: potato soaks juice 10～20%M/V, peptone 0.1～1%, glucose 1～10%, KH
2PO
40.01～0.1%, MgSO
40.01～0.1%, NaCl 0.005～0.1%, all the other are water, shake-flask culture carried out 1～10 hour with 30～80rmp/ component velocity under 20～30 ℃, and carried out flask culture 1～10 with same medium again with this liquid spawn, bacterial classification inoculation after the above-mentioned cultivation is carried out fermentation culture in fermentor tank, its nutrient solution is a soybean cake powder 5～15%, peptone 0.1～1%, yeast powder 0.01～0.5%, corn steep liquor 0.01～0.5%, sucrose 1～5%, Semen Maydis powder 1～5%, KH
2PO
40.01～0.5%, MgSO
40.01～0.1%, MaCl 0.05～0.1%, stirring velocity is 50～150rmp/ branch, cultivates that centrifugation goes out thalline after 1～10 day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96115299A CN1060518C (en) | 1996-05-10 | 1996-05-10 | Method for producing dictyophora indusiata nutritive mycelium using bamboo fungus submerged culture fermentation technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96115299A CN1060518C (en) | 1996-05-10 | 1996-05-10 | Method for producing dictyophora indusiata nutritive mycelium using bamboo fungus submerged culture fermentation technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1165187A CN1165187A (en) | 1997-11-19 |
| CN1060518C true CN1060518C (en) | 2001-01-10 |
Family
ID=5122573
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96115299A Expired - Fee Related CN1060518C (en) | 1996-05-10 | 1996-05-10 | Method for producing dictyophora indusiata nutritive mycelium using bamboo fungus submerged culture fermentation technology |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104557167A (en) * | 2015-01-07 | 2015-04-29 | 江南大学 | Liquid culture medium for submerged bamboo fungus fermentation |
| CN105000975A (en) * | 2015-07-06 | 2015-10-28 | 合肥福泉现代农业科技有限公司 | Dictyophora indusiata high-permeation moisture-retention medium completely prepared from cultivation waste of JUNCAO Ganoderma lucidum and preparation method thereof |
| CN105237126A (en) * | 2015-08-27 | 2016-01-13 | 马鞍山市安康菌业有限公司 | Pennisetum giganteum and mushroom bran dictyophora high-effective culture medium and preparation method thereof |
| CN105876033A (en) * | 2016-06-21 | 2016-08-24 | 福建农林大学 | Bamboo fungus-buckwheat tea and preparation method thereof |
| CN106858510A (en) * | 2017-01-18 | 2017-06-20 | 华南理工大学 | A kind of feature dictyophora phalloidea soy sauce compound seasoner and preparation method thereof |
| CN108531405A (en) * | 2018-04-09 | 2018-09-14 | 保定康而沃生物科技有限公司 | Promote the resistant to pollution complex enzyme of edible fungus culturing energy-saving and production-increase and preparation method |
| CN108967049A (en) * | 2018-07-05 | 2018-12-11 | 贵州省农作物品种资源研究所 | A kind of Dictyophora rubrovalvata liquid spawn quick-breeding method |
| CN112262958A (en) * | 2020-10-26 | 2021-01-26 | 织金县腾朋绿色食品有限公司 | Bamboo fungus noodles and preparation method thereof |
| CN115554195A (en) * | 2022-11-09 | 2023-01-03 | 湖南科技大学 | Composition for promoting eyebrow growth and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1038566A (en) * | 1989-05-09 | 1990-01-10 | 刘克均 | Super-clean seed preparation technique of distyophora phalloidea |
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1996
- 1996-05-10 CN CN96115299A patent/CN1060518C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1038566A (en) * | 1989-05-09 | 1990-01-10 | 刘克均 | Super-clean seed preparation technique of distyophora phalloidea |
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|---|---|
| CN1165187A (en) | 1997-11-19 |
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