CN106048766A - Anti-corrosive granulation-promoting fabric containing silk protein and preparation method thereof - Google Patents
Anti-corrosive granulation-promoting fabric containing silk protein and preparation method thereof Download PDFInfo
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- CN106048766A CN106048766A CN201610371074.5A CN201610371074A CN106048766A CN 106048766 A CN106048766 A CN 106048766A CN 201610371074 A CN201610371074 A CN 201610371074A CN 106048766 A CN106048766 A CN 106048766A
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- extract
- fibroin
- fabric containing
- erosion prevention
- folium camelliae
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
- D01F4/02—Monocomponent artificial filaments or the like of proteins; Manufacture thereof from fibroin
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0069—Electro-spinning characterised by the electro-spinning apparatus characterised by the spinning section, e.g. capillary tube, protrusion or pin
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
- D01F1/103—Agents inhibiting growth of microorganisms
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- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Mechanical Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacturing & Machinery (AREA)
- Dispersion Chemistry (AREA)
- Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
- Artificial Filaments (AREA)
Abstract
The invention provides a method for preparing an anti-corrosive granulation-promoting fabric containing silk protein. The method comprises the steps of taking abandoned silk as a raw material, removing impurities from the abandoned silk and then cutting the abandoned silk short to form staple silk fiber; removing impurities from tea, cinnamomum camphora and angelica sinensis, respectively grinding the tea, cinnamomum camphora and angelica sinensis into powder; through extracting solution extraction, separation, filtration and concentration, obtaining tea extract, cinnamomum camphora extract and angelica sinensis extract; adding the tea extract, the cinnamomum camphora extract, the angelica sinensis extract and calamine powder into the silk protein, performing stirring fully, adding natto antifungal protein, performing stirring to obtain spinning solution, placing the spinning solution in a syringe, and obtaining the anti-corrosive granulation-promoting fabric containing the silk protein through an electrospinning technique.
Description
Technical field
The invention belongs to textile material technical field, be specifically related to a kind of erosion prevention and muscle growing fabric containing fibroin and system thereof
Preparation Method.
Background technology
Along with economic develops rapidly, people improve the most rapidly for the requirement of living standard, to the many-side in life
Pursuing higher standard, green science and technology environmental protection is capable of circulation increasingly to be paid close attention to by people.Traditional weaving face fabric only focuses on face
The color and luster of material itself, comfortable and warmth retention property, along with the progress of science and technology, functional more and more higher to fabric now.
In daily work with life, often can cause skin damage because of unexpected, if impaired skin by physics,
The reasons such as chemistry, microorganism infection, can not heal for a long time, even cause circulation obstruction of limbs, and ischemic hypoxia is finally led
Cause skin and necrosis of subcutaneous tissue.The most conventional Therapeutic Method to skin damage is with the preferable face of breathability after painting is applied ointment
Material wraps up, the conventional gauze that fabric is cotton.Existing parcel gauze is only to gauze disinfection, and it is functional
Not good enough, therefore exploitation have antibacterial anticorrosion promote wound healing fabric there is certain market.
At present, promote based on antibacterial anticorrosion that the research in terms of the fabric of wound healing is actually rare.Chinese patent
A kind of medicine bed sore mattress disclosed in CN2136026Y, by by Radix Angelicae Sinensis, Rhizoma Chuanxiong, Jing Qiao, Ramulus Cinnamomi, Flos Carthami, Folium Bambusae, Folium Mori, Herba Dendranthematis indici
Hua He, Lasiosphaera Seu Calvatia Chinese medicine is made powder, is placed in fabric as inner core, makes fabric have after being wrapped up by Chinese medicinal powder rubber
Blood circulation promoting and blood stasis dispelling, the effect of removing toxic substances and promoting granulation.Additionally, there has been considerable research to find, animal proteinum can be had by many materials
There is certain antibacterial bacteriostatic effect.China's document (" the anticorrosion research of hydrolytic collagen solution ", Wang Bi etc., Chinese leather, the 32nd
Rolled up for the 9th phase, in May, 2003) disclosed in the food conventional preservative fungistatic effect to hydrolytic collagen solution, find Pyrusussuriensis
Hydrolytic collagen solution can be played bacteriostasis by acid, sodium benzoate, benzoic acid and propylparaben.Chinese patent CN
A kind of compound antibacterial finish agent and method for sorting thereof disclosed in 104294588A, this compound antibacterial finish agent includes nano-pearl
Powder, citric acid, chitosan, penetrating agent and water, this finishing agent can strengthen the metabolism of skin, promote reepithelialization,
Fabric can have certain antibacterial functions after this finishing agent Final finishing.From above-mentioned prior art, can be filled out by Chinese medicine
The method filling fabric or fabric post-treatment carries out the arrangement of antibacterial anticorrosion promotion skin regeneration to fabric, but by these two kinds
Active component in fabric prepared by method is limited, and the thickness of fabric prepared of these two kinds of methods and ventilative comfort property are difficult
To ensure, the healing to skin is not highly beneficial.
Applicant of the present invention, based on the understanding to prior art, selects hydrolyzed protein, plant extraction liquid and organic substance
As raw material, prepare through electrostatic spinning technique and there is the non-woven fabric that antibacterial bacteriostatic performance is excellent, and this fabric can be very
The good healing promoting wound surface skin.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of erosion prevention and muscle growing fabric containing fibroin and preparation method thereof,
Use fibroin, Folium Camelliae sinensis extract, natto antibacterial protein, Radix Cinnamomi porrecti extract, Radix Angelicae Sinensis extract and Calamina as raw material warp
Electrostatic spinning technique prepares the erosion prevention and muscle growing fabric containing fibroin.The fibroin contained in fabric prepared by the method
Collaborative with natto antibacterial protein can produce significant antiseptic effect, extract and Calamina in Folium Camelliae sinensis, Radix Cinnamomi porrecti and Radix Angelicae Sinensis are permissible
Play the effect promoting promoting tissue regeneration and wound healing, fabric green pro-skin, no side effects, can be used for weaving face fabric, biologic medical field.
For solving above-mentioned technical problem, the technical scheme is that
A kind of erosion prevention and muscle growing fabric containing fibroin, the described erosion prevention and muscle growing fabric containing fibroin includes silkworm silk egg
In vain, Folium Camelliae sinensis extract, natto antibacterial protein, Radix Cinnamomi porrecti extract, Radix Angelicae Sinensis extract and Calamina, described fibroin mainly includes
Serine, alanine and glycine, described Folium Camelliae sinensis extract is tea polyphenols, and described Calamina is powder, and particle diameter is 1-3 μm.
Preferred as technique scheme, each component in the described erosion prevention and muscle growing fabric containing fibroin, by weight
Meter, including fibroin 100 parts, Folium Camelliae sinensis extract 3-8 part, natto antibacterial protein 1-3 part, Radix Cinnamomi porrecti extract 5-10 part, Radix Angelicae Sinensis
Extract 6-15 part and Calamina 15-25 part.
The present invention also provides for the preparation method of a kind of erosion prevention and muscle growing fabric containing fibroin, it is characterised in that: include with
Lower step:
(1) using useless silkworm silk as raw material, the short silk of silkworm silk forming a length of 1-3cm after useless silkworm silk is removed impurity, is prescinded,
After high steam pretreatment, it is dissolved in the enzyme hydrolyzate of 2-3mg/ml with the solid-liquid ratio of 1g:20-30ml, after constant temperature hydrolysis, high temperature
Inactivation obtains the fibroin solutions that mass fraction is 5-8%；
(2) after Folium Camelliae sinensis, Radix Cinnamomi porrecti, Radix Angelicae Sinensis being gone the removal of impurity, grind into powder respectively, add first with 1g:5-10ml solid-liquid ratio
In alcohol extraction solvent, heated and stirred, supersound extraction, separating and filtering concentrates, respectively obtains the Folium Camelliae sinensis that mass fraction is 5-10% and carry
Take thing, mass fraction is the Radix Cinnamomi porrecti extract of 5-10% and mass fraction is the Radix Angelicae Sinensis extract of 5-10%；
(3) in fibroin prepared by step (1) add step (2) prepare Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, when
Return extract and Calamina powder, be sufficiently stirred for, add natto antibacterial protein, be uniformly mixing to obtain spinning solution；
(4) spinning solution prepared by step (3) is placed in syringe, obtains containing fibroin through electrostatic spinning technique
Erosion prevention and muscle growing fabric.
Preferred as technique scheme, in described step (1), autoclaved condition is: pressure 1.5-3mPa,
Time 30-60min.
Preferred as technique scheme, in described step (1), the temperature of constant temperature hydrolysis is 50-60 DEG C, and the time is
90-120min。
Preferred as technique scheme, in described step (2), the particle diameter of powder is 1-5 μm.
Preferred as technique scheme, in described step (2), the temperature of heated and stirred is 40-55 DEG C, heated and stirred
Time be 30-60min, the time of supersound extraction is 15-30min.
Preferred as technique scheme, in described step (3), the component in spinning solution by weight, including
Fibroin 100 parts, Folium Camelliae sinensis extract 3-8 part, natto antibacterial protein 1-3 part, Radix Cinnamomi porrecti extract 5-10 part, Radix Angelicae Sinensis extract 6-
15 parts and Calamina 15-25 part.
Preferred as technique scheme, in described step (3), the structural viscosity index △ η of spinning solution is 0.8-
0.9。
Preferred as technique scheme, in described step (4), the condition of electrostatic spinning is: voltage 15-25kV, connects
Being 20-25cm by distance, pushing speed is 25-30 μ l/min.
Compared with prior art, the method have the advantages that
(1) containing fibroin and natto antibacterial protein in the erosion prevention and muscle growing fabric containing fibroin prepared by the present invention,
Serine, glycine and the alanine contained in fibroin with skin contact after hydrolyzable separate peptide molecule, polypeptide divides
Containing various bioactivators in son, the activity of human skin can be promoted, reach the effect of Antimicrobial preservative, and natto is antibacterial
Albumen promotes the hydrolysis of fibroin, strengthens the activity of active substance, can be with fibroin generation synergism.
(2) in the erosion prevention and muscle growing fabric containing fibroin prepared by the present invention containing Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, when
Returning extract and Calamina, wherein Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, Radix Angelicae Sinensis extract broadly fall into plant extract, extract
All contain bacteriostasis antibiosis composition with Calamina, and skin is had convergence protective effect, environmental protection.
(3) the erosion prevention and muscle growing fabric containing fibroin prepared by the present invention has the antimicrobial preservative efficacy of excellence, moreover it is possible to promote
Enter promoting tissue regeneration and wound healing, and main component fibroin good biocompatibility, with contact skin without repelling sense, comfortable ventilating.
Detailed description of the invention
The present invention is described in detail, in illustrative examples and the explanation of this present invention below in conjunction with specific embodiment
It is used for explaining the present invention, but not as a limitation of the invention.
Embodiment 1:
(1) using useless silkworm silk as raw material, the short silk of silkworm silk forming a length of 1-3cm after useless silkworm silk is removed impurity, is prescinded,
After high steam pretreatment, it is dissolved in the enzyme hydrolyzate of 2mg/ml with the solid-liquid ratio of 1g:20ml, constant temperature hydrolysis at a temperature of 50 DEG C
After 90min, high-temperature inactivation 30min under 1.5mPa pressure, obtain the fibroin solutions that mass fraction is 5%.
(2) grind to form the powder that particle diameter is 1 μm after Folium Camelliae sinensis being gone the removal of impurity, add methanol extraction with 1g:5ml solid-liquid ratio molten
In agent, in 40 DEG C of heated and stirred 30min, supersound extraction 15min, separating and filtering is concentrated to give the Folium Camelliae sinensis that mass fraction is 5% and carries
Take thing, after Radix Cinnamomi porrecti is gone the removal of impurity, grind to form the powder that particle diameter is 1 μm, add in methanol extraction solvent with 1g:5ml solid-liquid ratio,
40 DEG C of heated and stirred 30min, supersound extraction 15min, separating and filtering be concentrated to give mass fraction be mass fraction be the perfume (or spice) of 5%
Cinnomomum Sps. Extract.Radix Angelicae Sinensis goes the removal of impurity grind to form powder that particle diameter is 1 μm, adds methanol extraction solvent with 1g:5ml solid-liquid ratio
In, in 40 DEG C of heated and stirred 30min, supersound extraction 15min, separating and filtering is concentrated to give the Radix Angelicae Sinensis that mass fraction is 5% and extracts
Thing.
(3) in fibroin prepared by step (1) add step (2) prepare Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, when
Return extract and Calamina powder, be sufficiently stirred for, add natto antibacterial protein, be uniformly mixing to obtain structural viscosity index △ η
Being the spinning solution of 0.8, wherein the component in spinning solution is by weight, including fibroin 100 parts, Folium Camelliae sinensis extract
Part, natto antibacterial protein 1 part, Radix Cinnamomi porrecti extract 5 parts, Radix Angelicae Sinensis extract 6 parts and Calamina 15 parts.
(4) spinning solution prepared by step (3) is placed in syringe, under 15kV voltage, with the propelling movement of 25 μ l/min
Speed, the distance at 20cm accepts 30min, obtains the erosion prevention and muscle growing fabric containing fibroin.
Embodiment 2:
(1) using useless silkworm silk as raw material, the short silk of silkworm silk forming a length of 1-3cm after useless silkworm silk is removed impurity, is prescinded,
After high steam pretreatment, it is dissolved in the enzyme hydrolyzate of 3mg/ml with the solid-liquid ratio of 1g:30ml, constant temperature hydrolysis at a temperature of 60 DEG C
After 120min, high-temperature inactivation 60min under 3mPa pressure, obtain the fibroin solutions that mass fraction is 8%.
(2) grind to form the powder that particle diameter is 5 μm after Folium Camelliae sinensis being gone the removal of impurity, add methanol extraction with 1g:10ml solid-liquid ratio
In solvent, in 55 DEG C of heated and stirred 60min, supersound extraction 30min, separating and filtering is concentrated to give the Folium Camelliae sinensis that mass fraction is 10%
Extract, grinds to form the powder that particle diameter is 5 μm after Radix Cinnamomi porrecti goes the removal of impurity, add methanol extraction solvent with 1g:10ml solid-liquid ratio
In, 55 DEG C of heated and stirred 60min, supersound extraction 30min, separating and filtering be concentrated to give mass fraction be mass fraction be 10%
Radix Cinnamomi porrecti extract.Radix Angelicae Sinensis goes the removal of impurity grind to form powder that particle diameter is 5 μm, adds methanol extraction with 1g:10ml solid-liquid ratio
In solvent, in 55 DEG C of heated and stirred 60min, supersound extraction 30min, it is working as of 5-10% that separating and filtering is concentrated to give mass fraction
Return extract.
(3) in fibroin prepared by step (1) add step (2) prepare Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, when
Return extract and Calamina powder, be sufficiently stirred for, add natto antibacterial protein, be uniformly mixing to obtain structural viscosity index △ η
Being the spinning solution of 0.9, wherein the component in spinning solution is by weight, including fibroin 100 parts, Folium Camelliae sinensis extract 8
Part, natto antibacterial protein 3 parts, Radix Cinnamomi porrecti extract 10 parts, Radix Angelicae Sinensis extract 15 parts and Calamina 25 parts.
(4) spinning solution prepared by step (3) is placed in syringe, under 25kV voltage, with the propelling movement of 30 μ l/min
Speed, the distance at 25cm accepts 60min, obtains the erosion prevention and muscle growing fabric containing fibroin.
Embodiment 3:
(1) using useless silkworm silk as raw material, the short silk of silkworm silk forming a length of 1-3cm after useless silkworm silk is removed impurity, is prescinded,
After high steam pretreatment, it is dissolved in the enzyme hydrolyzate of 2.5mg/ml with the solid-liquid ratio of 1g:25ml, thermostatted water at a temperature of 55 DEG C
After solving 100min, high-temperature inactivation 50min under 2mPa pressure, obtain the fibroin solutions that mass fraction is 6%.
(2) grind to form the powder that particle diameter is 2 μm after Folium Camelliae sinensis being gone the removal of impurity, add methanol extraction with 1g:8ml solid-liquid ratio molten
In agent, in 45 DEG C of heated and stirred 40min, supersound extraction 20min, separating and filtering is concentrated to give the Folium Camelliae sinensis that mass fraction is 6% and carries
Take thing, after Radix Cinnamomi porrecti is gone the removal of impurity, grind to form the powder that particle diameter is 3 μm, add in methanol extraction solvent with 1g:7ml solid-liquid ratio,
40 DEG C of heated and stirred 60min, supersound extraction 25min, separating and filtering be concentrated to give mass fraction be mass fraction be the perfume (or spice) of 7%
Cinnomomum Sps. Extract.Radix Angelicae Sinensis goes the removal of impurity grind to form powder that particle diameter is 2 μm, adds methanol extraction solvent with 1g:6ml solid-liquid ratio
In, in 50 DEG C of heated and stirred 45min, supersound extraction 20min, separating and filtering is concentrated to give the Radix Angelicae Sinensis that mass fraction is 7% and extracts
Thing.
(3) in fibroin prepared by step (1) add step (2) prepare Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, when
Return extract and Calamina powder, be sufficiently stirred for, add natto antibacterial protein, be uniformly mixing to obtain structural viscosity index △ η
Being the spinning solution of 0.85, wherein the component in spinning solution is by weight, including fibroin 100 parts, Folium Camelliae sinensis extract 6
Part, natto antibacterial protein 2 parts, Radix Cinnamomi porrecti extract 6 parts, Radix Angelicae Sinensis extract 8 parts and Calamina 19 parts.
(4) spinning solution prepared by step (3) is placed in syringe, under 20kV voltage, with the propelling movement of 26 μ l/min
Speed, the distance at 22cm accepts 45min, obtains the erosion prevention and muscle growing fabric containing fibroin.
Embodiment 4:
(1) using useless silkworm silk as raw material, the short silk of silkworm silk forming a length of 1-3cm after useless silkworm silk is removed impurity, is prescinded,
After high steam pretreatment, it is dissolved in the enzyme hydrolyzate of 2mg/ml with the solid-liquid ratio of 1g:30ml, constant temperature hydrolysis at a temperature of 55 DEG C
After 110min, high-temperature inactivation 40min under 2mPa pressure, obtain the fibroin solutions that mass fraction is 7%.
(2) grind to form the powder that particle diameter is 3 μm after Folium Camelliae sinensis being gone the removal of impurity, add methanol extraction with 1g:8ml solid-liquid ratio molten
In agent, in 50 DEG C of heated and stirred 40min, supersound extraction 15min, separating and filtering is concentrated to give the Folium Camelliae sinensis that mass fraction is 6% and carries
Take thing, after Radix Cinnamomi porrecti is gone the removal of impurity, grind to form the powder that particle diameter is 2 μm, add in methanol extraction solvent with 1g:8ml solid-liquid ratio,
50 DEG C of heated and stirred 40min, supersound extraction 20min, separating and filtering be concentrated to give mass fraction be mass fraction be the perfume (or spice) of 8%
Cinnomomum Sps. Extract.Radix Angelicae Sinensis goes the removal of impurity grind to form powder that particle diameter is 4 μm, adds methanol extraction solvent with 1g:9ml solid-liquid ratio
In, in 55 DEG C of heated and stirred 55min, supersound extraction 20min, separating and filtering is concentrated to give the Radix Angelicae Sinensis that mass fraction is 7% and extracts
Thing.
(3) in fibroin prepared by step (1) add step (2) prepare Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, when
Return extract and Calamina powder, be sufficiently stirred for, add natto antibacterial protein, be uniformly mixing to obtain structural viscosity index △ η
Being the spinning solution of 0.83, wherein the component in spinning solution is by weight, including fibroin 100 parts, Folium Camelliae sinensis extract 5
Part, natto antibacterial protein 2 parts, Radix Cinnamomi porrecti extract 7 parts, Radix Angelicae Sinensis extract 12 parts and Calamina 23 parts.
(4) spinning solution prepared by step (3) is placed in syringe, under 15-25kV voltage, with 25-30 μ l/min
Pushing speed, the distance at 20-25cm accepts 30-60min, obtains the erosion prevention and muscle growing fabric containing fibroin.
Embodiment 5:
(1) using useless silkworm silk as raw material, the short silk of silkworm silk forming a length of 1-3cm after useless silkworm silk is removed impurity, is prescinded,
After high steam pretreatment, it is dissolved in the enzyme hydrolyzate of 3mg/ml with the solid-liquid ratio of 1g:30ml, constant temperature hydrolysis at a temperature of 50 DEG C
After 90min, high-temperature inactivation 30min under 3mPa pressure, obtain the fibroin solutions that mass fraction is 8%.
(2) grind to form the powder that particle diameter is 2 μm after Folium Camelliae sinensis being gone the removal of impurity, add methanol extraction with 1g:8ml solid-liquid ratio molten
In agent, in 45 DEG C of heated and stirred 45min, supersound extraction 20min, separating and filtering is concentrated to give the Folium Camelliae sinensis that mass fraction is 8% and carries
Take thing, after Radix Cinnamomi porrecti is gone the removal of impurity, grind to form the powder that particle diameter is 3 μm, add in methanol extraction solvent with 1g:6ml solid-liquid ratio,
45 DEG C of heated and stirred 40min, supersound extraction 20min, separating and filtering be concentrated to give mass fraction be mass fraction be the perfume (or spice) of 7%
Cinnomomum Sps. Extract.Radix Angelicae Sinensis goes the removal of impurity grind to form powder that particle diameter is 1.5 μm, adds methanol extraction with 1g:10ml solid-liquid ratio molten
In agent, in 55 DEG C of heated and stirred 30min, supersound extraction 30min, separating and filtering is concentrated to give the Radix Angelicae Sinensis that mass fraction is 9% and carries
Take thing.
(3) in fibroin prepared by step (1) add step (2) prepare Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, when
Return extract and Calamina powder, be sufficiently stirred for, add natto antibacterial protein, be uniformly mixing to obtain structural viscosity index △ η
Being the spinning solution of 0.88, wherein the component in spinning solution is by weight, including fibroin 100 parts, Folium Camelliae sinensis extract 8
Part, natto antibacterial protein 3 parts, Radix Cinnamomi porrecti extract 8 parts, Radix Angelicae Sinensis extract 8 parts and Calamina 16 parts.
(4) spinning solution prepared by step (3) is placed in syringe, under 20kV voltage, with the propelling movement of 25 μ l/min
Speed, the distance at 23cm accepts 40min, obtains the erosion prevention and muscle growing fabric containing fibroin.
Embodiment 6:
(1) using useless silkworm silk as raw material, the short silk of silkworm silk forming a length of 3cm, warp after useless silkworm silk is removed impurity, are prescinded
After high steam pretreatment, it is dissolved in the enzyme hydrolyzate of 3mg/ml with the solid-liquid ratio of 1g:20ml, constant temperature hydrolysis at a temperature of 55 DEG C
After 100min, high-temperature inactivation 40min under 2.5mPa pressure, obtain the fibroin solutions that mass fraction is 7%.
(2) grind to form the powder that particle diameter is 2.5 μm after Folium Camelliae sinensis being gone the removal of impurity, add methanol extraction with 1g:8ml solid-liquid ratio
In solvent, in 45 DEG C of heated and stirred 50min, supersound extraction 25min, separating and filtering is concentrated to give the Folium Camelliae sinensis that mass fraction is 6%
Extract, grinds to form the powder that particle diameter is 2 μm after Radix Cinnamomi porrecti goes the removal of impurity, add methanol extraction solvent with 1g:6ml solid-liquid ratio
In, 50 DEG C of heated and stirred 50min, supersound extraction 25min, separating and filtering be concentrated to give mass fraction be mass fraction be 7%
Radix Cinnamomi porrecti extract.Radix Angelicae Sinensis goes the removal of impurity grind to form powder that particle diameter is 3 μm, adds methanol extraction with 1g:10ml solid-liquid ratio
In solvent, in 45 DEG C of heated and stirred 50min, supersound extraction 20min, separating and filtering is concentrated to give the Radix Angelicae Sinensis that mass fraction is 6%
Extract.
(3) in fibroin prepared by step (1) add step (2) prepare Folium Camelliae sinensis extract, Radix Cinnamomi porrecti extract, when
Return extract and Calamina powder, be sufficiently stirred for, add natto antibacterial protein, be uniformly mixing to obtain structural viscosity index △ η
Being the spinning solution of 0.86, wherein the component in spinning solution is by weight, including fibroin 100 parts, Folium Camelliae sinensis extract 7
Part, natto antibacterial protein 1 part, Radix Cinnamomi porrecti extract 6 parts, Radix Angelicae Sinensis extract 13 parts and Calamina 21 parts.
(4) spinning solution prepared by step (3) is placed in syringe, under 18kV voltage, with the propelling movement of 28 μ l/min
Speed, the distance at 23cm accepts 50min, obtains the erosion prevention and muscle growing fabric containing fibroin.
After testing, the healing of the grammes per square metre of erosion prevention and muscle growing fabric containing fibroin that prepared by embodiment 1-6, antibiotic rate, granulation promoting
The result of rate is as follows:
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | |
| Grammes per square metre (g/m2) | 98 | 132 | 110 | 116 | 118 | 124 |
| Antibiotic rate (%) | 89 | 91 | 86 | 95 | 92 | 93 |
| Healing rate (%) after treatment 7d | 85 | 86 | 79 | 81 | 85 | 83 |
As seen from the above table, the anti-corrosive antibacterial excellent performance of the erosion prevention and muscle growing fabric containing fibroin prepared by the present invention, also
There is certain promoting tissue regeneration and wound healing function.
The principle of above-described embodiment only illustrative present invention and effect thereof, not for limiting the present invention.Any ripe
Above-described embodiment all can be modified under the spirit and the scope of the present invention or change by the personage knowing this technology.Cause
This, have usually intellectual such as complete with institute under technological thought without departing from disclosed spirit in art
All equivalences become are modified or change, and must be contained by the claim of the present invention.
Claims (10)
1. the erosion prevention and muscle growing fabric containing fibroin, it is characterised in that: in the described erosion prevention and muscle growing fabric containing fibroin
Including fibroin, Folium Camelliae sinensis extract, natto antibacterial protein, Radix Cinnamomi porrecti extract, Radix Angelicae Sinensis extract and Calamina, described silkworm silk egg
Mainly including that serine, alanine and glycine, described Folium Camelliae sinensis extract are tea polyphenols in vain, described Calamina is powder, grain
Footpath is 1-3 μm.
A kind of erosion prevention and muscle growing fabric containing fibroin the most according to claim 1, it is characterised in that described containing silkworm silk egg
Each component in white erosion prevention and muscle growing fabric, by weight, resists including fibroin 100 parts, Folium Camelliae sinensis extract 3-8 part, natto
Mycoprotein 1-3 part, Radix Cinnamomi porrecti extract 5-10 part, Radix Angelicae Sinensis extract 6-15 part and Calamina 15-25 part.
3. the preparation method of the erosion prevention and muscle growing fabric containing fibroin, it is characterised in that comprise the following steps:
(1) using useless silkworm silk as raw material, the short silk of silkworm silk forming a length of 1-3cm is prescinded after useless silkworm silk is removed impurity, through height
After pressure steam pre-treatment, it is dissolved in the enzyme hydrolyzate of 2-3mg/ml with the solid-liquid ratio of 1g:20-30ml, after constant temperature hydrolysis, high-temperature inactivation
Obtain the fibroin solutions that mass fraction is 5-8%；
(2) after Folium Camelliae sinensis, Radix Cinnamomi porrecti, Radix Angelicae Sinensis being gone the removal of impurity, grind into powder respectively, add methanol with 1g:5-10ml solid-liquid ratio and carry
Take in solvent, heated and stirred, supersound extraction, separating and filtering concentrates, respectively obtain Folium Camelliae sinensis extract that mass fraction is 5-10%,
Mass fraction is the Radix Cinnamomi porrecti extract of 5-10% and mass fraction is the Radix Angelicae Sinensis extract of 5-10%；
(3) add Folium Camelliae sinensis extract prepared by step (2) in fibroin step (1) prepared, Radix Cinnamomi porrecti extract, Radix Angelicae Sinensis carry
Take thing and Calamina powder, be sufficiently stirred for, add natto antibacterial protein, be uniformly mixing to obtain spinning solution；
(4) spinning solution prepared by step (3) is placed in syringe, obtains the anticorrosion containing fibroin through electrostatic spinning technique
Granulation promoting fabric.
The preparation method of a kind of erosion prevention and muscle growing fabric containing fibroin the most according to claim 3, it is characterised in that: institute
Stating in step (1), autoclaved condition is: pressure 1.5-3mPa, time 30-60min.
The preparation method of a kind of erosion prevention and muscle growing fabric containing fibroin the most according to claim 3, it is characterised in that: institute
Stating in step (1), the temperature of constant temperature hydrolysis is 50-60 DEG C, and the time is 90-120min.
The preparation method of a kind of erosion prevention and muscle growing fabric containing fibroin the most according to claim 3, it is characterised in that: institute
Stating in step (2), the particle diameter of powder is 1-5 μm.
The preparation method of a kind of erosion prevention and muscle growing fabric containing fibroin the most according to claim 3, it is characterised in that: institute
Stating in step (2), the temperature of heated and stirred is 40-55 DEG C, and the time of heated and stirred is 30-60min, and the time of supersound extraction is
15-30min。
The preparation method of a kind of erosion prevention and muscle growing fabric containing fibroin the most according to claim 3, it is characterised in that: institute
State in step (3), the component in spinning solution by weight, including fibroin 100 parts, Folium Camelliae sinensis extract 3-8 part, natto
Antibacterial protein 1-3 part, Radix Cinnamomi porrecti extract 5-10 part, Radix Angelicae Sinensis extract 6-15 part and Calamina 15-25 part.
The preparation method of a kind of erosion prevention and muscle growing fabric containing fibroin the most according to claim 3, it is characterised in that: institute
Stating in step (3), the structural viscosity index △ η of spinning solution is 0.8-0.9.
The preparation method of a kind of erosion prevention and muscle growing fabric containing fibroin the most according to claim 3, it is characterised in that:
In described step (4), the condition of electrostatic spinning is: voltage 15-25kV, accepts distance for 20-25cm, and pushing speed is 25-30 μ
l/min。
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| CN107287688A (en) * | 2017-06-26 | 2017-10-24 | 苏州威尔德工贸有限公司 | A kind of manufacture method of anti bacteria natural silk composite fibre |
| CN107557891A (en) * | 2017-08-14 | 2018-01-09 | 浙江依爱夫游戏装文化产业有限公司 | A kind of preparation method based on the anti-corrosion fabric containing fibroin |
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| CN106048766B (en) | 2018-06-15 |
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