CN106047891A - Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof - Google Patents
Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof Download PDFInfo
- Publication number
- CN106047891A CN106047891A CN201610482535.6A CN201610482535A CN106047891A CN 106047891 A CN106047891 A CN 106047891A CN 201610482535 A CN201610482535 A CN 201610482535A CN 106047891 A CN106047891 A CN 106047891A
- Authority
- CN
- China
- Prior art keywords
- drought
- breeding
- ir66897b
- gene
- tolerant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 34
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 30
- 235000009566 rice Nutrition 0.000 title claims abstract description 20
- 230000024346 drought recovery Effects 0.000 title claims abstract description 10
- 240000007594 Oryza sativa Species 0.000 title claims description 15
- 238000000034 method Methods 0.000 title abstract description 22
- 239000003147 molecular marker Substances 0.000 title abstract description 7
- 238000009395 breeding Methods 0.000 claims abstract description 25
- 230000001488 breeding effect Effects 0.000 claims abstract description 24
- 241000209094 Oryza Species 0.000 claims abstract description 19
- 108700028369 Alleles Proteins 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 7
- 238000002372 labelling Methods 0.000 claims description 6
- 239000008280 blood Substances 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000003550 marker Substances 0.000 abstract description 4
- 238000009394 selective breeding Methods 0.000 abstract description 3
- 230000009466 transformation Effects 0.000 abstract description 2
- 230000007547 defect Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
- 238000005204 segregation Methods 0.000 abstract 1
- 230000002269 spontaneous effect Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 16
- 238000012360 testing method Methods 0.000 description 7
- 210000000349 chromosome Anatomy 0.000 description 5
- 230000008641 drought stress Effects 0.000 description 5
- 230000002349 favourable effect Effects 0.000 description 5
- 230000000306 recurrent effect Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 230000035882 stress Effects 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 2
- 238000009412 basement excavation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 240000008467 Oryza sativa Japonica Group Species 0.000 description 1
- 101100029854 Oryza sativa subsp. japonica PIP1-3 gene Proteins 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and a molecular marker method thereof, belonging to the fields of rice high-yield stress-tolerant breeding and molecular genetics. By utilizing Jijing 88 and 3 donor-derived breeding population selection, a novel segregation distortion locating process suitable for breeding population selection is adopted to locate drought-tolerance-related QTL (quantitative trait loci); and random population selection is utilized to verify the authenticity of the drought-tolerant QTL and the reliability of the close linkage marker. When being applied to auxiliary selective breeding and polymerization breeding of drought-tolerant rice, the gene can effectively overcome the defects in the past spontaneous mutant genes, can obviously lower the negative influence on the auxiliary breeding due to the development of the initial locating result, and can carry out genotype selection on the lower-generation breeding populations in the seedling stage to obtain the drought-tolerance individuals, thereby facilitating the timely hybrid transformation, omitting the drought resistance identification process in the adult-plant stage, enhancing the breeding efficiency and accelerating the breeding progress.
Description
Technical field
The present invention relates to one and dramatically increase Oryza sativa L. Drought-tolerant gene in reproduction period QDTY 2.9IR66897BAnd molecular marker side
Method, this gene significantly reduces Oryza sativa L. production loss under drought stress, belongs to rice high yield breeding for stress tolerance and molecular genetics
Field, it is adaptable to introduce in rice high yield breeding for stress tolerance at Drought-tolerant gene QDTY 2.9 in reproduction periodIR66897B, and utilize molecule mark
Note carries out assisted selection to this gene.
Background technology
Arid is to cause Rice Yield Loss Caused, limits one of most important abiotic stress factor of Rice Cropping distribution.When
Before, arid frequently occurs, and brings about great losses View of World Rice yield every year.But world wide population is but quickly increasing,
Grain demand breach is huge, therefore by excavating the favourable drought resisting allele in existing germ plasm resource, by molecule auxiliary choosing
It is the most urgent important scientific problems that means of selecting cultivate drought-enduring new varieties.Molecule genetics research shows, Oryza sativa L. is to arid
Resistance is the quantitative trait by controlled by multiple genes.The QTL that existing a large amount of drought tolerance are relevant so far is positioned, some and arid phase
The gene closed is also by successful clone.
Objectively, the excavation of resistance related gene contributes to it is understood that the Oryza sativa L. hereditary basis to drought resistance, but
The example of the real successful Application in the practices of breeding of most genes is the most extremely limited.One important limiting factor is basis
There is space in scientific research and breeding practice research, is connected the tightst.In order to overcome this problem, we have developed a set of new
Positioning strategy, i.e. uses selection and use colony to carry out drought-enduring quantitative gene excavation.Compared with conventional genetic colony, selection and use group
Body has two significant advantages；(1) individual in population quantity is relatively small, and phenotype can save to become in a large number with genotype identification
Basis and resource；(2) through artificial selection, the performance of part strain Main Agronomic is similar to recurrent parent, but in yield more
Tool advantage, therefore these strains carrying a large amount of favorable allels can be directly used as the parent of pyramiding breeding, even may be used
Directly selection-breeding becomes new lines.
Selection and use colony is made up of 3 familys, and each family shares a recurrent parent Ji round-grained rice 88.(P1 arrives 3 familys
P4) male parent is IR66897B, MR77 and MR167 respectively.These 3 male parents are local varieties sub-southeast, are respectively provided with one
Fixed drought tolerance.Utilizing the method that is partially located separately being suitable for selection and use colony, we are successfully realized the location of drought-enduring QTL
Research.Result shows, is positioned at the position existence one of the 2nd end of chromosome 192.2cM and a drought-enduring relevant gene loci
QDTY2.9.This QTL is all positioned in P1 and P3 colony.Further, we to use traditional random population to carry out above-mentioned
The checking work of QTL.This checking colony is each from 3 familys to randomly choose 60 strains, 180 strains compositions altogether.
The result shows, QDTY 2.9IR66897BIt is the anti-drought gene of necessary being, itself and molecular marker RM266 close linkage, and resistance to
Drought favorable allels is from drought-enduring parent IR66897B and MR167.By with the drought resisting QTL reported and gene physical location
Compare, find QDTY 2.9IR66897BAdjacent with the Drought-tolerant Gene OsPIP1-3 (RWC3) cloned, thus it is speculated that QDTY
2.9IR66897BIt is probably OsPIP1-3 or its allele.But OsPIP1-3 utilizes reverse genetics means to clone, and does not still have
Have and develop the molecular marker that can be directly used for drought-enduring molecule assisted selection.QDTY 2.9 in the present inventionIR66897BResistance
Allele subrace IR66897B and MR167 southeast, can be that drought-enduring breeding provides available kind of new matter, is conducive to
Expand China's kind or plant the genetic diversity of matter.
Summary of the invention
In order to overcome the deficiencies in the prior art, it is an object of the invention to provide Oryza sativa L. Drought-tolerant gene in a reproduction period QDTY
2.9IR66897BAnd molecule labelling method.The present invention utilizes the method that is partially located separately being suitable for selection and use colony, in Oryza sativa L.
The location positioning of the 2nd end of chromosome 192.2cM to one and a drought-enduring relevant gene loci QDTY2.9.It is drought-enduring has
Profit allele is from drought-enduring parent IR66897B, with molecular marker RM266 close linkage.The Practical economy mark of PCR-based
Note RM266, utilizes it can effectively carry out the research of Oryza sativa L. drought-enduring molecule in reproduction period assisted selection.
Technical scheme is as follows: Oryza sativa L. Drought-tolerant gene in reproduction period QDTY 2.9IR66897B, at rice genome the 2nd
The position existence one and a drought-enduring relevant gene loci QDTY2.9 of end of chromosome 192.2cM, the two of Asia southeast
Individual drought tolerant germplasm IR66897B and MR167 allele on this site can dramatically increase drought-enduring in reproduction period of Oryza sativa L.
Property.
QDTY 2.9IR66897BMolecule labelling method, it is characterised in that: by a pair special PCR primer to RM266, its
Middle forward primer sequence is: TAGTTTAACCAAGACTCTC, and reverse primer sequences is: GGTTGAACCCAAATCTGCA, jointly
Expand with the breeding material genomic DNA of kind IR66897B blood relationship, if primer RM266 can be amplified by PCR and
The fragment of about the 117bp size that IR66897B is similar, then speculate that this breeding material is likely to containing QDTY 2.9IR66897B's
Drought-enduring allele.
Drought-tolerant gene in the reproduction period (QDTY 2.9 on the 2nd chromosome is identified by the present inventionIR66897B) and can be to it
Carry out the molecular method utilized.The present invention compared with prior art has the following advantages and effect:
1., based on reverse genetics means, it is positioned at QDTY 2.9IR66897BUpstream juxtaposition is equipped with a Drought-tolerant Gene
OsPIP1-3 is cloned, thus it is speculated that QDTY 2.9IR66897BIt is probably OsPIP1-3 or its allele.Forward genetics is utilized to learn to do
Section, present invention obtains and QDTY 2.9IR66897BClosely linked common molecular labelling RM266, by compact linkage molecule mark
The screening of note, it is possible to the readily available breeding material that reproduction period, drought tolerance strengthened.
2.QDTY 2.9IR66897BDrought-enduring favorable allels from International Rice high-quality drought tolerant germplasm IR66897B
With Malaysia local varieties MR167, different from the donor kind of OsPIP1-3, provide new available kind for drought-enduring breeding
Matter, is conducive to expanding China's kind or planting the genetic diversity of matter.
3. the molecular marker of the present invention can be used for the genotype selection of breeding population in seedling stage, effectively differentiates with this gene
Drought-enduring type individual, it is simple to hybridize transformation timely, accelerate breeding process.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to
Other accompanying drawing is obtained according to these accompanying drawings.
Tu1Shi P1 colony parent and the random individual plant electrophoretogram in RM266 site.(M、P1And P2Represent marker respectively,
Lucky round-grained rice 88, and IR66897B；1-10 represents 10 individual plants randomly choosed from P1 colony).
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1
(1) location of drought-enduring QTL
1. material to be tested
First with screening from 3 Southeast Asia local varieties IR66897B (P1 colony male parent) of germplasm resource bank, MR77 (P2
Colony's male parent) and MR167 (P3 colony male parent) build 3 high generations with China high yield and high quality the most drought-enduring japonica rice Ji round-grained rice 88 respectively and backcross
Introgressive line colony.After two-wheeled drought stress in reproduction period selects, 72 the drought-enduring strains filtered out in 3 familys constitute selection
Breeding population (table 1).
1.3 selection and use Canopy structure information of table
N1It is the original BC without drought stress2F2Group size；N2It is to select after first round drought stress processes
Drought-enduring strain number；N3It is to take turns, through 2, the drought-enduring strain number filtered out after drought stress processes；.
2. genotype identification
With reference to the DNA extraction method of (2000) such as Temnykh, each individual plant is extracted genomic DNA respectively.With donor parent
Polymorphic SSR primer is had respectively the genotype of the offspring that different familys derive to be identified between this and recurrent parent Ji round-grained rice 88.3
The polymorphism primer number of individual family is respectively 181,201 and 197.
The most drought-enduring assessment
Drought-enduring qualification test is at Chinese Academy of Agricultural Sciences's Changping County, Beijing Experimental Base and Ningxia Academy of Agri-Forestry Sciences's Experimental Base
Complete.Each test site all includes matched group and two parts of process group.Matched group is all taked normally to fill at whole period of duration
Water management, process group then then begins to take on Osmotic treatment until seed full maturity children in the fringe sprouting stage.Comparison and process are all
Use the completely random district group test of 3 repetitions.Using single plant yield as evaluation index.
The most partially separate analysis (QTL location)
Owing to 72 strains are made up of different 3 different familys, between different familys, polymorphism mark there are differences, therefore I
Be first according to the method for (2015) such as Cui and developed a conforming genetic linkage maps.And then use (2015) such as Cui
The method that is partially located separately provided carries out the location of drought-enduring QTL.It is 16.97 as the mark judging QTL presence or absence using Wald value
Accurate.
Reproduction period, drought-enduring QTL identified.3 familys navigate to 7 drought tolerances QTL altogether.Wherein it is positioned at the 2nd chromosome 2 to contaminate
QDTY2.9 on the position of colour solid end 192.2cM is all navigated to by P1 and P2 Liang Ge colony, and sub-southeast two drought-enduring
Plant matter IR66897B and MR167 allele on this site can dramatically increase the Oryza sativa L. drought tolerance in reproduction period.
The location of the drought-enduring QTLQDTY of table 2 2.9
P value=0.01 when Wald value=20.35；P value=0.05 when Wald value=16.97；
(2) checking of QDTY 2.9
1. material to be tested
From original 3 advanced backcross analysis without Stress treatment and artificial selection (800 strains of each colony)
Randomly choose 3 accidental validation colonies of 60 individual plant groups respectively.
2. genotype identification
The CTAB method using routine extracts the genomic DNA of Oryza sativa L. individual plant.With between donor parents and recurrent parent Ji round-grained rice 88
Polymorphic SSR primer is had respectively the genotype of the offspring that different familys derive to be identified.Use the Standard PCR of 20 μ l systems
The template DNA of the different individual plant of operating process amplification, carries out the separation of PCR primer with polyacrylate hydrogel electrophoresis.
The most drought-enduring phenotypic assessment
Drought-enduring qualification test is carried out in Chinese Academy of Agricultural Sciences's Changping County, Beijing proving ground and Ningxia academy of agricultural sciences Experimental Base.
The test of each test site all includes matched group and process group two parts.The whole period of duration of matched group all takes normal field to manage
Reason, and process group i.e. starts Osmotic treatment until seed full maturity children in the fringe sprouting stage.Comparison and process all use 3 repetitions
The test of completely random district group.Using single plant yield as evaluation index.
3. One marker analysis
60 strains of each family are divided by the genotype represented by RM266 labelling amplified band according to different strains
Becoming two groups, one group is the individuality with lucky round-grained rice 88 homozygous genotype；Another group is the individuality with donor homozy genotype.Dan Biao
Note analysis result shows, QDTY 2.9 is positioned in P1 and P3 colony again, the average individual plant in two places under drought stress
Units increased in production reaches 23.5%, is the drought resisting QTL of a main effect, and resistance favorable allels is all from donor parents.This shows
QDTY 2.9IR66897BIt is the drought-enduring QTL of a necessary being, also indicates that RM226 really and QDTY 2.9 close linkage, can simultaneously
Directly apply to drought resisting molecule assisted selection.
Table 3.QDTY 2.9IR66897BAnd the checking of linked marker RM266
HDY, the average single plant yield of donor gene type plant of isozygotying；HRY, the average individual plant of recipient genotypes plant of isozygotying
Yield；RPY, the average single plant yield of recurrent parent Ji round-grained rice 88；A, additive effect.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of the spirit or essential attributes of the present invention, it is possible to realize the present invention in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive.The scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all by fall in the implication of equivalency and scope of claim
Change is included in the present invention.Should not be considered as limiting involved claim by any reference in claim.
Claims (3)
1. one kind dramatically increases Oryza sativa L. Drought-tolerant gene in reproduction period QDTY2.9IR66897B, it is characterised in that: contaminate at rice genome the 2nd
Position existence one and a drought-enduring relevant gene loci QDTY2.9 of colour solid end 192.2cM, sub-southeast two
Drought tolerant germplasm IR66897B and MR77 allele on this site can dramatically increase the Oryza sativa L. drought tolerance in reproduction period.
2. Drought-tolerant gene QDTY2.9 as claimed in claim 1IR66897BMolecule labelling method, it is characterised in that: special with a pair
Different PCR primer is to RM266, and wherein forward primer sequence is: TAGTTTAACCAAGACTCTC, and reverse primer sequences is:
GGTTGAACCCAAATCTGCA, coamplification with the breeding material genomic DNA of kind IR66897B and MR167 blood relationship, as
Really primer RM266 can amplify the fragment of about the 117bp size similar with IR66897B and MR167 by PCR, then pushes away
Survey this breeding material to be likely to containing QDTY2.9IR66897BDrought-enduring allele.
3. according to the application in rice breeding of the Drought-tolerant gene described in claim 1,2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610482535.6A CN106047891A (en) | 2016-06-27 | 2016-06-27 | Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610482535.6A CN106047891A (en) | 2016-06-27 | 2016-06-27 | Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106047891A true CN106047891A (en) | 2016-10-26 |
Family
ID=57166846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610482535.6A Withdrawn CN106047891A (en) | 2016-06-27 | 2016-06-27 | Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106047891A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108034755A (en) * | 2018-01-16 | 2018-05-15 | 中国农业科学院作物科学研究所 | Chain molecular labeling and its application with rice Drought-tolerant gene qLRI9 phases |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1952144A (en) * | 2005-10-18 | 2007-04-25 | 华中农业大学 | Promoting growth of root and/or improving drought resistance of plant by using paddy gene OsRRG1 |
| CN1952142A (en) * | 2005-10-17 | 2007-04-25 | 华中农业大学 | Improvement of plant drought resistance by using paddy pathogenesis-related gene OsPR4-1 |
-
2016
- 2016-06-27 CN CN201610482535.6A patent/CN106047891A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1952142A (en) * | 2005-10-17 | 2007-04-25 | 华中农业大学 | Improvement of plant drought resistance by using paddy pathogenesis-related gene OsPR4-1 |
| CN1952144A (en) * | 2005-10-18 | 2007-04-25 | 华中农业大学 | Promoting growth of root and/or improving drought resistance of plant by using paddy gene OsRRG1 |
Non-Patent Citations (1)
| Title |
|---|
| 崔彦茹 等: "利用水稻选择育种群体定位抗旱QTL", 《中国作物学会-2015年学术年会论文摘要集》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108034755A (en) * | 2018-01-16 | 2018-05-15 | 中国农业科学院作物科学研究所 | Chain molecular labeling and its application with rice Drought-tolerant gene qLRI9 phases |
| CN108034755B (en) * | 2018-01-16 | 2020-05-08 | 中国农业科学院作物科学研究所 | Molecular marker linked with rice drought-enduring gene qLRI9 and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Navabi et al. | Introgression of B-genome chromosomes in a doubled haploid population of Brassica napus× B. carinata | |
| Iftekharuddaula et al. | Development of new submergence tolerant rice variety for Bangladesh using marker-assisted backcrossing | |
| Persson et al. | Genetic sex determination and RAPD marker segregation in the dioecious species sea buckthorn (Hippophae rhamnoides L.) | |
| CN102154471B (en) | Molecular marking method for major quantitative trait loci(QTL) for rice grain length | |
| CN105441457B (en) | A kind of QTL molecule labelling method relevant to low nitrogen Rice under Condition mass of 1000 kernel | |
| CN109924121A (en) | A method of improvement Rice Resistance To Rice Blast | |
| CN104805212B (en) | PCR label for screening fertility restorer gene Rfo of Ogura cytoplasmic sterile line of Brassica oleracea var.capitata L. | |
| CN102121052B (en) | Specific molecular marker sequence for identifying recessive genic male sterility mutant gene ms 26 and its wild type allele | |
| CN104830967A (en) | Positioning method of rice selected introgression lines QTL | |
| Gao et al. | Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye | |
| CN108157165A (en) | A kind of method using Rice Genomics technology fast accurate selection and breeding three lines paddy sterile line | |
| CN106047891A (en) | Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof | |
| CN111341384A (en) | Quantitative Trait Locus (QTL) sites of soybean and screening method thereof | |
| Li et al. | A new gene for hybrid sterility from a cross between Oryza sativa and O. glaberrima | |
| CN114480709B (en) | Molecular marker for detecting wheat leaf rust resistance gene Lr47, detection method and application thereof | |
| CN105671039B (en) | The molecular labeling indel15-1 of soybean early flowering season main effect QTL and its application | |
| CN104762298A (en) | Rice seedling-stage salt-tolerant gene qST11 and molecular marker method thereof | |
| CN108342506B (en) | Molecular marker of cabbage type rape MI CMS system restoring gene Rfm and application thereof | |
| CN108085404A (en) | Giant pumpkin female trait molecular marker and the primer pair of identification giant pumpkin female character by force by force | |
| CN105420357B (en) | General SSR marker closely linked with dominant nuclear male sterility gene in broccoli | |
| CN106434687A (en) | Gene QDTY11.5<IR66897B> for substantially enhancing drought tolerance of rice in reproductive stage, and molecular marking method thereof | |
| CN104032021B (en) | SNP molecular labeling primer and the application thereof in a kind of pear fruit seed amount main effect QTL site | |
| CN102605074A (en) | Molecular marker method for identifying BT type cytoplasmmale sterility restoring geneRfla of japonica rice | |
| CN102925433A (en) | Wheat unreduced gamete gene molecular marker and application thereof | |
| CN101880710A (en) | SCAR molecular marker interlocked with brown planthopper resistance genes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20161026 |