CN106045629A - Azadirachtin fertilizer and application thereof in improvement on content of squalene in tobacco leaves - Google Patents
Azadirachtin fertilizer and application thereof in improvement on content of squalene in tobacco leaves Download PDFInfo
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- CN106045629A CN106045629A CN201610447077.2A CN201610447077A CN106045629A CN 106045629 A CN106045629 A CN 106045629A CN 201610447077 A CN201610447077 A CN 201610447077A CN 106045629 A CN106045629 A CN 106045629A
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- azadirachta indica
- fertile
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- nicotiana tabacum
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses an azadirachtin fertilizer and application thereof in improvement on the content of squalene in tobacco leaves. The azadirachtin fertilizer is prepared from the following components: 30 to 60 percent of azadirachta indica seed and the balance of diatomite. The azadirachtin fertilizer is applied in a growth process of tobacco, so the content of the squalene in the tobacco leaves can be remarkably improved, and economic value of the tobacco leaves is improved.
Description
Technical field
The present invention relates to tobacco planting field, more particularly, to a kind of Azadirachta Indica A. fertilizer and contain improving Nicotiana tabacum L. Squalene
Application in amount.
Background technology
Squalene is a kind of lipid non-saponifiable matter, for a kind of many unsaturated olefins (squalene), is initially from shark
Liver oil in find, within 1914, be named as Squalene, its chemical name is 2,6,10,15,19,23-hexamethyl-2,6,
10,14,18,22-tetracosa carbon six alkene, belong to open chain triterpene, also known as Squalene, participate in human body cholesterol biosynthesis and can promote new
Old metabolism, has raising internal superoxide dismutase (SOD) activity, enhancing human body immunity ability, defying age, resisting fatigue, resists
The different physiological roles such as tumor, are a kind of avirulent marine biomaterial with the effect of preventing and curing diseases.
Squalene is widely present in animal and plant body.In animal the highest with content in deepwater shark liver, mainly deep
In the armour shark in sea, spinule shark, edge kiss Tian Shi shark, New Zealand's crow shark liver, and the Squalene content of other sea, river shark is the lowest, general
All over less than 1.5%.In plant then the highest, as olive oil contains Squalene with content in olive oil, Amaranthus mangostanus L. seed oil and Petiolus Trachycarpi oil
150～700mg/100g, in Testa oryzae oil, its content is 332mg/100g.Deepwater shark is precious protection animal, adds it
Low rate of growth and low reproductive capacity, easy overfishing, cause the Animal resources containing Squalene to lack.Have now been found that containing Squalene
Plant resources accumulation is few, develops and has certain difficulty.Thus limit production and the use of Squalene.
Prior art has had open extraction Squalene from the plants such as Fructus Momordicae, tail alpine ash, glutinous rice scented tea, Nicotiana tabacum L..
Also someone reports that extracting Squalene from tobacco waste sees ZL201110058487.5；Therefore extracting Squalene in plant will be not
Obtain an important channel of Squalene.But in various plants, the content of Squalene is the highest, does not also have in prior art
Research shows to improve the report of Squalene content in plant by adding exogenous material in the plant growing stage.
Summary of the invention
It is an object of the invention to provide a kind of Azadirachta Indica A. fertile.In the growth course of Nicotiana tabacum L., use Azadirachta Indica A. fertile, it is possible to increase cigarette
The content of Squalene in leaf.
The invention provides a kind of Azadirachta Indica A. fertile, described Azadirachta Indica A. fertilizer is that neem seed mixes with kieselguhr.
What a kind of Azadirachta Indica A. that the present invention provides was fertile preferably constitute for, percentage component consists of: neem seed
30～60%, surplus is kieselguhr.More preferably neem seed powder 50-60%, surplus is kieselguhr.
In the present invention, neem seed used can be commercially to obtain.
Preferably, described neem seed is the neem seed dry powder after pulverizing.It is highly preferred that described neem seed dry powder is
Cross the neem seed dry powder of 20 mesh sieves.
Invention also provides the fertile application in improving Nicotiana tabacum L. Squalene content of Azadirachta Indica A..Preferably, it is preferable that described
The application process that Azadirachta Indica A. is fertile is that root is executed.
Preferably, the amount of application that described Azadirachta Indica A. is fertile is 40～80 grams/strain.More preferably 60-80 gram/strain.
Preferably, the fertile fast growing period Nicotiana tabacum L. of described Azadirachta Indica A. is used once.
Preferably, described Azadirachta Indica A. fertilizer is used once after tobbaco.
Preferably, the fertile fast growing period Nicotiana tabacum L. of described Azadirachta Indica A. is respectively used once after pinching.
Inventor finds in the growth course of Nicotiana tabacum L., uses Azadirachta Indica A. fertile, it is possible to increase the content of Squalene in Nicotiana tabacum L..Cigarette
Leaf is in addition to the preparation being mainly used in medicated cigarette, and it can produce substantial amounts of such as waste products such as offal, waste tobacco leaf, inapplicable Nicotiana tabacum Lves, often
Often can only be processed by modes such as degradeds, cause waste.Squalene is a kind of material with economic worth, promotes Nicotiana tabacum L.
The content of middle Squalene, the most also improves the economic worth of Nicotiana tabacum L., makes the exploitation value of the waste product such as offal, waste tobacco leaf
It is worth promoting.
Compared with prior art, there is advantages that
Inventor finds, in the growth course of Nicotiana tabacum L., uses a certain amount of described Azadirachta Indica A. fertile, it is possible to increase angle in Nicotiana tabacum L.
The content of zamene, compared with blank group, the Squalene content that Azadirachta Indica A. fertilizer processes in Nicotiana tabacum L. is obviously improved, and improves cigarette
The economic worth of leaf.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further details.Embodiment is only used for explaining the present invention, and
Present disclosure is not limited in any form.
In embodiment, neem seed used is commercially available neem seed, limited purchased from the exploitation of Luxi City's light Neem industry
Responsible company.After being pulverized 20 mesh sieves, mix with kieselguhr.
Embodiment 1:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 30%, kieselguhr 70%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
In Nicotiana tabacum L. fast growing period: 1,40g/ strain applies Azadirachta Indica A. fertilizer；2,60g/ strain applies Azadirachta Indica A. fertilizer；3,80g/ strain applies Azadirachta Indica A. fertilizer；4、
80g/ strain kieselguhr；The quantity of every kind of situation process is 15 strains.
After Nicotiana tabacum L. maturation, every kind of situation selects 3 strains, and whole blades of every strain are cleaned, and blot surface moisture with gauze, in removing
Arteries and veins, 105 DEG C of 15min that complete, to dry to constant weight, pulverize, cross 40 mesh sieves for 60 DEG C, gained sample is used for measuring Squalene in Nicotiana tabacum L. and contains
Amount.
The mensuration of the absolute content of Squalene in Nicotiana tabacum L.
Weighing above-mentioned gained Nicotiana tabacum L. powder 20g filter paper parcel to load in apparatus,Soxhlet's, bath temperature is constant simultaneously is
75 DEG C, add 200mL petroleum ether (60～90 DEG C) and be heated at reflux 4h, be dried petroleum ether layer with anhydrous sodium sulfate, take petroleum ether and carry
Take liquid Rotary Evaporators to rotate to dry, obtain yellow leaf tobacco extract, be dissolved to 50mL with petroleum ether, utilize gas chromatogram, adopt
The absolute content (mg/Kg) of Squalene in Nicotiana tabacum L. is gone out with internal mark method determination.
GC conditions is as follows: HP-5MS capillary column (30m × 0.25mm × 0.25 μm), column temperature 150 DEG C, program liter
Temperature 15 DEG C/min to 290 DEG C, constant temperature 15min；Carrier gas is high-purity He；Inlet temperature 300 DEG C；Split ratio 20: 1；Solvent excises
1.5min；Sample size 0.5 μ L.
The mensuration of the relative amount of Squalene in flue gas
Reference literature (GC/MS method detects Squalene content [J] in 24 kinds of flue-cured tobaccos. guangdong agricultural science, and 2012,39
(3) tobacco heat desorption apparatus: 105-107.), is utilized to obtain the flue gas ligroin extraction of Nicotiana tabacum L. powder: Nicotiana tabacum L. powder is Nicotiana tabacum L.
Burning in thermal desorption device, the flue gas produced during burning is pumped into the cone equipped with petroleum ether sealed under the effect of vacuum pump
In shape bottle, the composition in flue gas is absorbed by petroleum ether.
Then the composition in the GC/MS flue gas to extracting is utilized to be analyzed measuring: to arrive according to Nist standard spectrum library searching
Qualitative results, determines its percentage composition according to mass spectrographic total ion current figure, and the workstation software carried according to mass spectrograph is returned
One changes mensuration, obtains the percentage contents (%) of Squalene in flue gas.
Its Mass Spectrometry Conditions is as follows: GC/MS interface temperature 290 DEG C；Ion source temperature 235 DEG C；Ionization mode EI source；Ionization electricity
Pressure 70eV；Mass scan range 30～500amu；SCAN (full scan) detects.
Test result is as shown in table 1 below:
Table 1
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 2:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 40%, kieselguhr 60%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
In Nicotiana tabacum L. fast growing period: 1,40g/ strain；2,60g/ strain；3,80g/ strain；4,80g/ strain kieselguhr；The quantity that every kind of situation processes
It is 15 strains.
The extraction of Squalene and detect same as in Example 1.
Test result is as shown in table 2 below:
Table 2
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 3:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 60%, kieselguhr 40%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
In Nicotiana tabacum L. fast growing period: 1,40g/ strain；2,60g/ strain；3,80g/ strain；4,80g/ strain kieselguhr；The quantity that every kind of situation processes
It is 15 strains.
The extraction of Squalene and detect same as in Example 1.
Test result is as shown in table 3 below:
Table 3
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 4:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 30%, kieselguhr 70%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
Fertilising in 1 week after tobbaco: 1,40g/ strain；2,60g/ strain；3,80g/ strain；4,80g/ strain kieselguhr；Every kind of situation process
Quantity is 15 strains.
The extraction of Squalene and detect same as in Example 1.
Test result is as shown in table 4 below:
Table 4
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 5:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 40%, kieselguhr 60%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
Fertilising in 1 week after tobbaco: 1,40g/ strain；2,60g/ strain；3,80g/ strain；4,80g/ strain kieselguhr；Every kind of situation process
Quantity is 15 strains.
The extraction of Squalene and detect same as in Example 1.
Test result is as shown in table 5 below:
Table 5
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 6:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 60%, kieselguhr 40%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
Fertilising in 1 week after tobbaco: 1,40g/ strain；2,60g/ strain；3,80g/ strain；4,80g/ strain kieselguhr；Every kind of situation process
Quantity is 15 strains.
The extraction of Squalene and detect same as in Example 1.
Test result is as shown in table 6 below:
Table 6
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 7:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 30%, kieselguhr 70%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
At Nicotiana tabacum L. fast band trophophase with within 1 week, respectively execute 1 fertilizer after pinching: 1,40g/ strain；2,60g/ strain；3,80g/ strain；4,80g/ strain diatom
Soil；The quantity of every kind of situation process is 15 strains.
The extraction of Squalene and detect same as in Example 1.
Test result is as shown in table 7 below:
Table 7
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 8:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 40%, kieselguhr 60%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
At Nicotiana tabacum L. fast band trophophase with within 1 week, respectively execute 1 fertilizer after pinching: 1,40g/ strain；2,60g/ strain；3,80g/ strain；4,80g/ strain diatom
Soil；The quantity of every kind of situation process is 15 strains.
The extraction of Squalene and detect same as in Example 1.
Test result is as shown in table 8 below:
Table 8
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Embodiment 9:
Selecting a field, community, flue-cured tobacco cultivars is cloud and mist 87, plants cigarette 60 strain, arranges according to 15 strains/row, distance between rows and hills be 1m ×
0.5m.Not using any insecticide during whole fertility, other cultivation management measure is with conventional Production of Large Fields.Azadirachta Indica A. fertile by with
The component composition of lower percetage by weight: neem seed powder 60%, kieselguhr 40%.Randomly choose Nicotiana tabacum L. strain to be handled as follows,
At Nicotiana tabacum L. fast band trophophase with within 1 week, respectively execute 1 fertilizer after pinching: 1,40g/ strain；2,60g/ strain；3,80g/ strain；4,80g/ strain diatom
Soil；The quantity of every kind of situation process is 15 strains.
The extraction of Squalene and detect same as in Example 1.
Test result is as shown in table 9 below:
Table 9
It can be seen that in Nicotiana tabacum L. fast growing period, after using Azadirachta Indica A. fertilizer, the Squalene content in Nicotiana tabacum L. gets a promotion.
Claims (12)
1. an Azadirachta Indica A. fertilizer, it is characterised in that percentage component consists of: neem seed 30～60%, surplus is
Kieselguhr.
Azadirachta Indica A. the most according to claim 1 is fertile, it is characterised in that percentage component consists of: neem seed
50～60%, surplus is kieselguhr.
Azadirachta Indica A. the most according to claim 1 and 2 is fertile, it is characterised in that described neem seed is the neem seed after pulverizing
Dry powder.
Azadirachta Indica A. the most according to claim 3 is fertile, it is characterised in that described neem seed dry powder was the Azadirachta Indica A. kind of 20 mesh sieves
Sub-dry powder.
5. according to the fertile application in improving Nicotiana tabacum L. Squalene content of described Azadirachta Indica A. arbitrary in claim 1-4.
Application the most according to claim 5, it is characterised in that the application process that described Azadirachta Indica A. is fertile is that root is executed.
Application the most according to claim 5, it is characterised in that the amount of application that described Azadirachta Indica A. is fertile is 40～80 grams/strain.
Application the most according to claim 7, it is characterised in that the amount of application that described Azadirachta Indica A. is fertile is 60～80 grams/strain.
Application the most according to claim 5, it is characterised in that described Azadirachta Indica A. fertilizer uses one at the quick trophophase of Nicotiana tabacum L.
Secondary.
Application the most according to claim 5, it is characterised in that described Azadirachta Indica A. fertilizer is used once after tobbaco.
11. application according to claim 5, it is characterised in that the fertile fast growing period Nicotiana tabacum L. of described Azadirachta Indica A. and pinching
After respectively use once.
12. application according to claim 5, it is characterised in that the application process that described Azadirachta Indica A. is fertile is that root is executed；Described Azadirachta Indica A.
Fertile amount of application is 80 grams/strain；The fertile fast growing period Nicotiana tabacum L. of described Azadirachta Indica A. respectively executes 1 fertilizer after pinching 1 week.
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| CN201610447077.2A CN106045629B (en) | 2016-06-21 | 2016-06-21 | It prints chinaberry fertilizer and its is improving the application in tobacco leaf squalene content |
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| CN201610447077.2A CN106045629B (en) | 2016-06-21 | 2016-06-21 | It prints chinaberry fertilizer and its is improving the application in tobacco leaf squalene content |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104045401A (en) * | 2014-07-03 | 2014-09-17 | 覃仁安 | Special tung-oil-containing compound fertilizer for tea trees |
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2016
- 2016-06-21 CN CN201610447077.2A patent/CN106045629B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104045401A (en) * | 2014-07-03 | 2014-09-17 | 覃仁安 | Special tung-oil-containing compound fertilizer for tea trees |
Non-Patent Citations (1)
| Title |
|---|
| 夏衍等: "中国华南不同生态区烤烟烟叶中角鲨烯积累与主要土壤生态因子关系的探讨", 《全国农药学科教育科研研讨会暨赵善欢学术思想与研究实践讨论会》 * |
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