CN1060407A - The preparation method of lactase-containing dried yeast - Google Patents
The preparation method of lactase-containing dried yeast Download PDFInfo
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- CN1060407A CN1060407A CN 90108094 CN90108094A CN1060407A CN 1060407 A CN1060407 A CN 1060407A CN 90108094 CN90108094 CN 90108094 CN 90108094 A CN90108094 A CN 90108094A CN 1060407 A CN1060407 A CN 1060407A
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- yeast
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- semipermeable materials
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Abstract
A kind of preparation method that contains disaccharidase dried yeast such as Lactose enzyme, this method will be induced the viable yeast lyophilization that produces enzyme, adopt semipermeable materials and hydrophobic material will contain enzyme yeast peplos granulating by the cold nebulization evaporation then, again compress tablet coating.Can protect the enzyme in the yeast not destroyed by Digestive system so effectively, and remove complicated enzyme extraction process from, this method is applicable to that also except that yeast other contain the microorganism formulation of medicinal enzyme.
Description
The invention belongs to the preparation technology of biochemical pharmaceutics, relate to the preparation method of disaccharidase enzyme dried yeasts such as a kind of pastille Lactose enzyme.
Asia and African ethnic group can cause the lactose dyspepsia because of digestive tract Lactose enzyme hyposecretion, symptom of digestive tract (flatulence, stomachache, diarrhoea etc.) occurs after causing edible milk, promptly are called " lactose intolerance " (LACtose intoberAnce).The survey showed that among China the Hans, and 80% above adult has lactase defect in various degree, in the infant also up to 70%.For addressing this problem, to Symptomatic patient when using milk oral by microbe-derived Lactose enzyme do substitute with aid digestion be a kind of feasible way.Except that lactose intolerance, disaccharidase enzyme (maltase and the saccharase etc.) hyposecretion that human body (especially infant) also can cause the intestinal mucosal injury secondary to cause to comprise Lactose enzyme because of bacterial enteritis causes long-term dyspepsia.Also available ectogenic disaccharidase enzyme preparation is done the substitute treatment to this.But, but the difficult point that addresses the above problem is microbial enzyme source and the suitable destructive oral enzyme preparation in digestion resistant road that a kind of safe high yield was not arranged in the past.Abroad once yeast was induced produce Lactose enzyme after, through extraction be made into powder, (spy opens clear 51 54081 to solution; The spy opens clear 51 9783; EP74,237 1983).Easily destroyed when its shortcoming is this class preparation oral, and the extraction separation method of Lactose enzyme is also comparatively complicated by gastric acid and gastrointestinal tract protease.Disaccharidase in the commercially available yeast tablet is also very easily destroyed at gastrointestinal tract, almost can not bring into play pharmacological action.
For addressing the above problem, the present invention proposes the method for the yeast that contains disaccharidasies such as Lactose enzyme directly being made the granule dosage form.Because adopt semipermeable materials and hydrophobic material as coated fertilizer, this enzyme yeast that contains can protect contained disaccharidase not destroyed by gastric acid stomach function regulating intestinal protease after taking effectively.
Yeast is the edible microorganismus that a class can produce disaccharidase, and saccharase and maltase is natural existence in yeast, and some yeast strain can also produce Lactose enzyme through inducing.To contain the yeast lyophilization of disaccharidasies such as Lactose enzyme.Make enzyme be retained in the yeast cells and keep greater activity.Dry yeast is suspended in the semipermeable materials solution then and wraps one deck semi permeability thin film, to prevent that entering the enzyme that includes behind the digestive tract is decomposed by trypsin by the cold nebulization method.With the peplos yeast particles tabletting that so obtains, and multiple bag can stop that the enteric coating that gastric acid penetrates makes enteric coated tablet.
Also can be directly in the semi permeability filmogen, add and to stop that the hydrophobic composition that hydrion penetrates makes the peplos yeast particles.Such preparation can stop that under one's belt hydrion penetrates the destruction disaccharidase, molten yeast disaccharidase function of removing to play behind the hydrophobic composition semipermeable materials coating in intestinal.
Except that yeast, said method is applicable to that fully other contain the preparation of medicinal enzyme microorganism formulation.Use the medicinal enzyme microorganism formulation that contains of this law preparation to have the intestinal protective value equally, and have anti-gastric acid infiltration performance concurrently.
Advantage of the present invention is to have removed the complicated technology that extracts disaccharidase from yeast from, and the biological activity of contained disaccharidase makes it not be destroyed at gastrointestinal tract in the protection yeast, gives full play to pharmacological action, and can utilize trace element and vitamin in the yeast.
This technology specific procedure is as follows:
One, zymic cultivation and induce the product enzyme: carry out according to a conventional method, the centrifugal or viable yeast that filters results Su Dong And is immediately made lyophilization.The dry yeast that obtains should be done enzyme assay and moisture determination.
Two, semipermeable materials (containing hydrophobic composition) parcel contains the enzyme dry yeast: adopt cellulose lipid (celluloid or cellulose acetate or ethyl cellulose) as the semipermeable membrane material; Adopt high melting point lipid (Chinese tallow, Cera Flava, hard ester acid etc.) as hydrophobic material.During preparation the lyophilizing yeast is suspended in the organic solvent that contains semipermeable materials and hydrophobic material, becomes granule in lower temperature (＜50 ℃) spray evaporation solvent primary drying.The particle size diameter of products therefrom is less than 100 microns, and film thickness is less than 0.5 micron.The acid energy of the thickness of the method scalable film of control solvent and solute ratio and yeast concentration, permeability and resistance.
Three, contain enzyme dry yeast granule and make enteric coated tablet: tabletting and coating method carry out routinely.Coating material is an enteric material: cellulose acetate-phthalate, Lac or zein etc.
Claims (4)
1, a kind of preparation method that contains the disaccharidase dried yeast; the dried yeast made from this method has that anti-gastric acid infiltrates performance and in the protective value of enteral to enzyme; it is characterized in that to contain lyophilization after the quick-freezing of enzyme viable yeast; again this lyophilizing yeast is mixed with semipermeable materials; spray evaporation peplos becomes granule under less than 50 ℃ of conditions, will use the enteric material coating behind the further tabletting of granule.
2,, it is characterized in that high melting point lipid added in the described semipermeable materials as hydrophobic material and mix with the lyophilizing yeast again, by cold nebulization evaporation peplos granulating according to the described preparation method that contains the enzyme dried yeast of claim 1.
3, according to claim 1 or the 2 described preparation methoies that contain the enzyme dry yeast, this method also is applicable to preparation, and other contain the microorganism formulation of medicinal enzyme.
4, according to claim 1 or the 2 described preparation methoies that contain the enzyme dry yeast, it is characterized in that: described semipermeable materials is celluloid or cellulose acetate or ethyl cellulose; Described high melting-point lipid is Chinese tallow or Cera Flava or stearic acid; Described enteric material is cellulose acetate-phthalate or Lac or zein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90108094 CN1060407A (en) | 1990-09-30 | 1990-09-30 | The preparation method of lactase-containing dried yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90108094 CN1060407A (en) | 1990-09-30 | 1990-09-30 | The preparation method of lactase-containing dried yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1060407A true CN1060407A (en) | 1992-04-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 90108094 Pending CN1060407A (en) | 1990-09-30 | 1990-09-30 | The preparation method of lactase-containing dried yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1060407A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995034292A2 (en) * | 1994-06-14 | 1995-12-21 | Recordati S.A. Chemical And Pharmaceutical Company | Stabilized biologically active compounds contained in coated microgranules which can be suspended in alimentary fluids |
| CN1067876C (en) * | 1995-07-28 | 2001-07-04 | 北京市营养源研究所 | Protective film of preparation for rumen |
-
1990
- 1990-09-30 CN CN 90108094 patent/CN1060407A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995034292A2 (en) * | 1994-06-14 | 1995-12-21 | Recordati S.A. Chemical And Pharmaceutical Company | Stabilized biologically active compounds contained in coated microgranules which can be suspended in alimentary fluids |
| WO1995034292A3 (en) * | 1994-06-14 | 2001-12-06 | Recordati Chem Pharm | Stabilized biologically active compounds contained in coated microgranules which can be suspended in alimentary fluids |
| CN1067876C (en) * | 1995-07-28 | 2001-07-04 | 北京市营养源研究所 | Protective film of preparation for rumen |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |