CN106036082B - Antibiotic-free feed for resisting porcine diarrhea - Google Patents
Antibiotic-free feed for resisting porcine diarrhea Download PDFInfo
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Abstract
The invention relates to an antibiotic-free feed prepared by adding compound bacterial liquid into batch mixture and performing solid state fermentation, wherein the compound bacterial liquid is prepared by mixing 1-3 parts by weight of trichoderma longibrachiatum bacterial liquid, 1-3 parts by weight of aspergillus niger bacterial liquid and 1-3 parts by weight of candida tropicalis bacterial liquid. Compared with the common feed for pigs, the antibiotic-free feed disclosed by the invention is reasonable in compatibility and obvious in curative effect, can be used for obviously reducing the diarrhea rate of pigs, and has a special effect on improving the diarrhea of pigs as proved by experiments; the feed has no western medicine antibiotic component, so the feed has no toxic or side effect, no drug resistance and no pollution.
Description
Technical Field
The invention relates to the field of feed, in particular to livestock antibiotic-free feed and application thereof.
Background
Since the middle of the 20 th century, the addition of antibiotics in the feed plays a positive role in preventing animal diseases, promoting animal growth, improving feed reward, improving the yield of livestock and poultry products and the like. With the wide application of antibiotics as feed additives, various disadvantages of antibiotics are known, such as causing the normal flora in the gastrointestinal tract of animals to be disordered and generating drug resistance. The long-term use of antibiotics results in a large amount of residues in meat and egg products in animal production, and the residues have toxicity, teratogenicity, carcinogenicity and mutagenicity to human bodies. The quality of the animal products is reduced, the residual toxicity damages the environment and threatens the health of human as animal product consumers, and meanwhile, the trade adverse difference of animal product export is formed, the difficulty is brought to the export foreign exchange of the animal products, and the economic benefit of the breeding industry is influenced to a great extent. The problem that the negative influence of antibiotics on livestock and poultry production is urgently needed to be solved by looking for green environment-friendly antibiotic substitutes and reducing the negative influence of the antibiotics on livestock and poultry production is solved.
The probiotic preparation has the advantages of no toxic or side effect, no drug resistance, no residue, remarkable effect, low cost and the like, can effectively supplement beneficial microorganisms in the digestive tract, kill harmful microorganisms, particularly pathogenic bacteria such as escherichia coli and the like, improve the flora balance of the digestive tract, and rapidly improve the disease resistance, metabolism and feed absorption capacity of the organism, thereby achieving the double effects of preventing and treating digestive tract diseases and promoting growth. The probiotic preparation has obvious action effect by unique action mode and natural components, and becomes an ideal antibiotic substitute.
The probiotic preparation is a product of probiotics, metabolites thereof and growth stimulating substances, which is prepared by biological engineering and microecological engineering under the guidance of microecological balance theory, microecological imbalance theory, microecological nutrition theory and microecological prevention and treatment theory, can survive through animal gastric acid, bile acid, pancreatic juice and other barriers, and finally colonize and propagate in the digestive tract to adjust the microecological imbalance in a host, keep the microecological balance, and improve the health level or health state of the host.
Microorganisms that can be used as probiotic preparations must meet several criteria: (1) firstly, the paint can not generate any toxin, namely is nontoxic, harmless, safe and free of side effect; (2) shows a certain resistance to the digestion of gastric acid, pepsin, trypsin and bile, so that the bile can survive in the digestive tract; (3) can adhere to the mucosa of the digestive tract and permanently or temporarily colonize the gastrointestinal tract; (4) is beneficial to promoting the flora balance in the host or preventing dysbiosis, and has certain promotion effect on the health of the host; (5) the product has clinical effectiveness when fed according to reasonable dosage; (6) the product can keep higher activity and longer stability during preparation and storage; (7) can survive in a variety of hosts. Finally, this criterion is probably most difficult to achieve, although most species have a relatively stable intestinal microflora, each species has its own distinct bacterial flora. Moreover, factors such as stress or antibiotic treatment can alter the microflora in the gut, causing a less dominant population or even pathogenic bacteria to become the dominant bacteria. Therefore, selection of strains is adapted to comprehensive consideration, and strains satisfying more of the above conditions are selected as much as possible.
Many probiotics are found in indigenous intestinal bacteria and have been shown to have clinical efficacy in some gastrointestinal disorders. Probiotics differ in their mechanism of action, except in clinical action and composition. The probiotic preparation can supplement normal microorganisms lacking in intestinal tracts in quantity or variety, adjust or maintain microecological balance in the intestinal tracts, enhance the immune function and promote the digestion and absorption of nutrient substances, thereby achieving the effects of preventing and treating diseases without diseases and improving the conversion rate of words and materials and the production performance of livestock and poultry. The results reported in the studies on microbial feed additives to date are very different and there are various explanations in theory. Through experience accumulated by research institutes on the animal husbandry of probiotic formulations, the mechanism of action of probiotic formulations is now summarized as follows:
1. Maintaining intestinal microecological balance: a large number of microbial flora exist in the gastrointestinal tract of animals and keep a relatively balanced and stable state. Some of these flora are important for the growth and development of animals and for the resistance to diseases. Normally, the dominant population in the intestinal tract of animals is anaerobic bacteria, accounting for over 99 percent, including lactobacillus, bifidobacterium, digestive bacillus, and eubacterium, etc., while aerobic bacteria and facultative anaerobic bacteria only account for 1 percent. Once the flora loses balance, the digestive tract skills of the animals are disordered, the production performance is reduced, pathogenic microorganisms can multiply to cause the animals to suffer from diseases, and at the moment, the probiotic preparation can effectively supplement the quantity of beneficial bacteria, inhibit the growth and the reproduction of pathogenic flora, and adjust and restore the flora balance of intestinal tracts. The mechanism of the animal body for maintaining intestinal microecological balance has the following two aspects: (1) resistance to permanent planting: the native species of the animal gastrointestinal tract inhibit colonization of the intestinal tract by other foreign microorganisms, which is resistance to colonization and is also known as competitive exclusion. (2) Generating antibacterial substances: for example, lactobacilli produce bacteriocins, bacteriocinoids and other metabolites with bacteriostatic action. Bacteriocins are protein bacteriostatic substances which are synthesized and secreted into the environment in the process of bacterial metabolism and have an inhibitory effect on strains with close relatives. Most of the known bacteriocins have bacteriostatic effects only on bacteria with the same gram stain, but as the research goes deep, many bacteriocins have been found to have a wide range of effects.
2. Biological oxygen deprivation: beneficial aerobic bacteria in the probiotics consume oxygen in the environment in the process of growth and reproduction in animal intestinal tracts, so that the concentration of local oxygen molecules can be reduced, the colonization and growth and reproduction of obligate anaerobic bacteria of normal flora are facilitated, the microecological balance of the intestinal tracts is recovered to be normal, and the purpose of preventing and treating diseases is achieved. Research results show that after 3 days of continuous oral administration of bacillus cereus to diarrhea piglets, bifidobacteria and lactobacilli in intestinal anaerobic bacteria are remarkably increased, and escherichia coli and salmonella are remarkably reduced. After the pigs are fed with the bacillus licheniformis, the anaerobic bacteria in the intestinal flora are increased, and the aerobic bacteria and the facultative anaerobic bacteria, particularly escherichia coli, are obviously reduced.
3. Biological antagonism: beneficial bacteria in the probiotic preparation have an antagonistic effect on pathogenic microorganisms in vivo, i.e., colonization resistance. This resistance to colonization is due to competition of the intestinal epithelium for adsorption sites by in vivo microorganisms and pathogenic bacteria. If these adsorption sites are occupied by more beneficial microorganisms, pathogenic microorganisms are rejected and excreted with the feces. Research reports indicate that after the bacillus is fed to the chicks, the bacillus has extremely remarkable antagonistic effect on escherichia coli and salmonella in the intestines of the chicks. In vitro test tube culture test, the lactobacillus microecological preparation has strong biological antagonism to pathogenic salmonella pullorum.
4. The immunity function of the organism is enhanced: some probiotic preparations are good immune activators, because the probiotic preparations are nonspecific immune regulating factors, the probiotic preparations can stimulate the immune system of the body to produce interferon, improve the concentration of immunoglobulin and the activity of macrophages, and improve the humoral immunity and cellular immunity level of animals, and the improvement of the immunity of the animals by the probiotic preparations is mainly reflected in the following aspects: (1) the research result shows that the lactobacillus can obviously enhance the activity of the macrophage. The Lactobacillus casei can improve the phagocytic activity of macrophages on Listeria; additional studies have shown that yeast extracts can potentiate the response of various macrophages; (2) effects on immune organs, antibody production levels, cytokine production; (3) activation and enhancement of specific immune responses.
5. Synthesizing digestive enzyme to promote digestion and absorption of nutrient substances: beneficial microorganisms in the probiotic preparation can generate hydrolase, digestive enzyme and the like, so that the complex carbohydrates such as protein, fat and the like in the feed can be degraded, and the feed conversion rate is improved. Aspergillus such as Aspergillus oryzae and Aspergillus niger can produce cellulase, and is favorable for digestion, absorption and utilization of cellulose by animals. Aspergillus niger is also capable of producing amylase, protease, pectinase. Some yeasts have the function of enriching trace elements, so that the yeasts are changed from an inorganic form into an organic form which is easy to digest and absorb by animals. After the acid-producing probiotics such as bacillus, lactobacillus and the like are used for feeding animals, the small intestine mucosa of the animals has more wrinkles, the continuous hair is lengthened, the mucosa gland fossa is deepened, and the small intestine absorption area is increased, so that the weight gain rate and the feed utilization rate are improved. In addition to stronger protease, amylase and lipase activities, bacillus subtilis and bacillus licheniformis also have enzymes for degrading some complex carbohydrates in plant feed, such as collagenase, glucanase, cellulase and the like, wherein a plurality of enzymes are enzymes which cannot be produced by mammals and poultry per se.
6. Improve the environment in the intestinal tract and reduce the generation of harmful substances such as ammonia, amine and the like: ammonia, amine, indole, hydrogen sulfide and other substances have obvious toxic action on animal intestinal mucosa cells. Lactic acid bacteria can generate inhibiting substances such as organic acid and bacteriocin in intestinal tract, inhibit the growth of putrefying bacteria such as intestinal colibacillus, reduce the activity of urease, further reduce the conversion of protein to harmful substances such as ammonia and amine, reduce the concentration of ammonia in the intestine, reduce excretion to the outside, and improve the living environment of livestock and poultry.
The probiotic preparations are formally divided into prebiotics, prebiotics and synbiotics internationally. The probiotics, namely the probiotics preparation in the narrow sense, is a viable bacteria preparation which is harmless to a host and can play a role in improving the ecological balance of intestinal flora of the host and a metabolite thereof. The probiotics currently applied to human bodies include lactic acid bacteria, bifidobacteria, enterococcus, escherichia coli, bacillus subtilis, bacillus licheniformis, bacillus cereus, clostridium butyricum, yeast and the like. The prebiotics are substances which can not be digested and absorbed by the host but can selectively promote the growth and reproduction of one or more original beneficial bacteria in the intestinal tract of the host, and the growth of harmful bacteria is inhibited by increasing the number of the beneficial bacteria, so that the aims of adjusting the intestinal flora, balancing the microecology of the intestinal tract and promoting the health of organisms are fulfilled. Such as bifidus factor, oligosaccharide or oligosaccharide. And certain Chinese herbal medicines such as ginseng, codonopsis pilosula, astragalus and the like or tea extracts in China can also play a role of prebiotics. The synbiotics refer to a preparation containing prebiotics and prebiotics. Such products are theoretically mixtures having both the above components and properties, but this definition is rarely adopted.
Probiotics can be mainly classified into three types at home and abroad, the first type is the general anaerobic and oxygen-resistant lactobacillus, such as lactobacillus acidophilus, lactobacillus casei and the like; the second is strictly anaerobic Bifidobacterium, such as Bifidobacterium longum, Bifidobacterium breve, etc.; the third category is facultative anaerobe, such as enterococcus faecalis, enterococcus lactis, etc. In addition, there are also yeasts and enzymes that fall under the category of probiotics. The probiotics commonly used in feed include lactic acid bacteria, bacillus, yeast, etc.
Lactic acid bacteria are a general term for a group of gram-positive bacteria that can utilize fermentable carbohydrates to produce large amounts of lactic acid. Lactic acid bacteria are defined as: the cells are rod-shaped or spherical, gram-positive, do not produce peroxidase, consume glucose and produce more than 50% lactic acid, do not form endospores, have no motility, or have only a few motility. The biological antagonistic action and the reduction of the pH value of the biological antagonistic bacterium can prevent and inhibit the invasion and colonization of pathogenic bacteria, degrade harmful substances such as ammonia, indole, skatole and the like in an animal body, maintain the normal ecological balance in an intestinal tract, and enhance humoral immunity and cellular immunity. The lactic acid bacteria can be used in feed for lactating and weaning animals. Lactic acid bacteria are widely distributed in nature, and not only exist in intestinal tracts and other organs of people and animals, but also exist in food, feed, soil, plant roots, rivers and lakes. The lactobacillus has high application value in the fields closely related to human beings such as industry, agriculture, food, medical care and the like, and can be used for producing probiotic preparations, lactic acid, silage inoculants, lactic acid fermented foods (such as fermented dairy products, fermented vegetables, fermented meat products and the like), bacteriocins, cosmetic moisturizers and the like. The lactic acid bacteria currently used are mainly microorganisms of nearly 30 species derived from the genus Lactobacillus, Streptococcus lactis and Bifidobacterium. Lactic acid bacteria allowed to be used as feed additives by the Ministry of agriculture in China are: 7 kinds of lactobacillus casei, lactobacillus plantarum, streptococcus faecalis, streptococcus faecium, pediococcus acidilactici, lactobacillus acidophilus, streptococcus lactis and the like.
Bacillus is a pass-through bacterium for the intestinal tract of probiotics and cannot colonize the intestinal tract. The bacillus subtilis is aerobic and harmless, can generate spores which are resistant to acid, alkali and high temperature and extrusion, has high stability in an intestinal acid environment, promotes the growth of beneficial bacteria, antagonizes harmful bacteria in the intestinal tract, enhances the immunity of the organism and improves the disease resistance; can secrete protease and amylase with strong activity, and can obviously improve the growth speed of animals and promote the digestion of nutrient substances of the feed. At present, more strains are reported to be beneficial strains such as bacillus subtilis, bacillus licheniformis, bacillus cereus and bacillus toyoja.
The yeast used in the probiotic preparation is one of active yeast preparation and one of yeast culture. The yeast cells are rich in protein, nucleic acid, vitamins and various enzymes, have the functions of enhancing the immunity of animals, improving the palatability of the feed, promoting the digestion and absorption capacity of the animals on the feed and the like, and can improve the utilization rate of the animals on phosphorus; the culture is rich in nutrients, vitamin B, mineral matter, digestive enzyme, growth promoting factor and complete amino acids. The application of yeast and its culture has been long, and it has been widely used in breeding of various animals, and the yeasts allowed by the department of agriculture at present are saccharomyces cerevisiae, brewer's yeast, etc.
At present, the application of compounding trichoderma longibrachiatum, aspergillus niger and candida tropicalis as a swine nonreactive feed additive is not reported.
Disclosure of Invention
The invention aims to solve the technical problem of providing the antibiotic-free feed for pigs, which can effectively improve the diarrhea of pigs, and has no toxic or side effect, no pollution and no drug resistance.
The technical scheme adopted by the invention for solving the problems is to provide the non-antibiotic feed for resisting the pig diarrhea, which is prepared by adding compound bacterial liquid into batch mixture and performing solid state fermentation, wherein the compound bacterial liquid is prepared by mixing 1-3 parts by weight of trichoderma longibrachiatum bacterial liquid, 1-3 parts by weight of aspergillus niger bacterial liquid and 1-3 parts by weight of candida tropicalis bacterial liquid.
Preferably, the compound bacterial liquid is prepared by compounding 1 part by weight of trichoderma longibrachiatum bacterial liquid, 3 parts by weight of aspergillus niger bacterial liquid and 1 part by weight of candida tropicalis bacterial liquid.
More preferably, the viable bacteria count of the three bacterial liquids per unit weight part is the same.
Preferably, the batch materials comprise soybean meal, corn protein powder, rapeseed meal, peanut meal, calcium hydrophosphate, salt and the like.
More preferably, the batch further comprises lysine, methionine, threonine, and the like.
More preferably, the batch further comprises vitamin A, vitamin C, vitamin E, vitamin B 12 and the like.
Most preferably, the batch mixture is prepared by uniformly mixing 40 parts by weight of soybean meal, 20 parts by weight of corn protein powder, 20 parts by weight of rapeseed meal, 6 parts by weight of peanut meal, 6 parts by weight of calcium hydrophosphate, 3 parts by weight of salt, 1 part by weight of lysine, 1 part by weight of methionine, 1 part by weight of threonine, 0.5 part by weight of vitamin A, 0.5 part by weight of vitamin C, 0.5 part by weight of vitamin E and 0.5 part by weight of vitamin B 12 0.5.
Preferably, the weight ratio of the batch to the composite bacterial liquid is (90-95) to (10-5).
More preferably, the weight ratio of the batch to the composite bacterial liquid is 95: 5.
The invention has the positive and beneficial effects that:
Surprisingly, through repeated experiments, the invention unexpectedly discovers that the antibiotic-free feed obtained by compounding the trichoderma longibrachiatum liquid, the aspergillus niger liquid and the candida tropicalis liquid and then performing solid state fermentation has a synergistic effect on preventing and treating the porcine diarrhea, and the using effect is better when the antibiotic-free feed is matched with other active ingredients.
Compared with the common feed for pigs, the antibiotic-free feed disclosed by the invention is reasonable in compatibility and obvious in curative effect, can be used for obviously reducing the diarrhea rate of pigs, and has a special effect on improving the diarrhea of pigs as proved by experiments; the feed has no western medicine antibiotic component, so the feed has no toxic or side effect, no drug resistance and no pollution.
Detailed Description
The present invention will be further described with reference to the following examples, but the embodiments of the present invention are not limited thereto. The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Example 1 preparation of Trichoderma longibrachiatum solution
Inoculating Trichoderma longibrachiatum (strain number ACCC30150) into PDA liquid culture medium, and culturing at constant temperature of 37 deg.C for 48h to obtain Trichoderma longibrachiatum bacterial liquid.
The PDA liquid culture medium is as follows: 200 g of potato, 20 g of glucose and 1000 ml of sterile water.
Example 2 preparation of Aspergillus niger liquid
Aspergillus niger (strain No. CICC40613) is inoculated on a Czapek's agar culture medium, cultured for 72h at 28 ℃, inoculated in a Czapek's liquid culture medium and cultured for 72h at the constant temperature of 28 ℃ to obtain an Aspergillus niger liquid.
The Czapek's agar medium was: 3 percent of sucrose, 0.3 percent of sodium nitrate, 0.05 percent of magnesium sulfate heptahydrate, 0.05 percent of potassium chloride, 0.001 percent of ferrous sulfate tetrahydrate, 0.1 percent of dipotassium hydrogen phosphate and 1.5 percent of agar, and the pH value is 6.0-6.5. The Czapek's liquid medium was obtained without agar.
Example 3 preparation of Candida tropicalis solution
Inoculating Candida tropicalis (strain number ACCC20005) to a wort agar culture medium, culturing at 28 ℃ for 72h, inoculating to a wort liquid culture medium, and culturing at 28 ℃ for 72h to obtain a Candida tropicalis bacterial liquid.
The wort agar culture medium is as follows: the wort is 5-6 Baume degree, pH 6.5, and agar 2g/100ml is added. The malt extract liquid culture medium is obtained without adding agar.
Example 4 preparation of batch
Taking 40 parts by weight of soybean meal, 20 parts by weight of corn protein powder, 20 parts by weight of rapeseed meal, 6 parts by weight of peanut meal, 6 parts by weight of calcium hydrophosphate, 3 parts by weight of salt, 1 part by weight of lysine, 1 part by weight of methionine, 1 part by weight of threonine, 0.5 part by weight of vitamin A, 0.5 part by weight of vitamin C, 0.5 part by weight of vitamin E and 0.5 part by weight of vitamin B 12 0.5, and uniformly mixing to obtain the batch mixture.
Example 5 preparation of antibiotic-free feed (A1-A6)
the trichoderma longibrachiatum bacterial liquid prepared in the example 1 is concentrated at a low temperature until the number of live bacteria in the bacterial liquid is about 10 8/g, the trichoderma longibrachiatum bacterial liquid is added into the batch prepared in the example 4 according to the inoculation amount of 5%, and solid state fermentation is carried out for 7d at the temperature of 35 ℃, so that the control non-antibacterial feed A1 is obtained.
The Aspergillus niger bacterial liquid prepared in the example 2 is concentrated at low temperature until the number of viable bacteria in the bacterial liquid is about 10 8/g, the Aspergillus niger bacterial liquid is added into the batch prepared in the example 4 according to the inoculation amount of 5%, and the mixture is subjected to solid state fermentation for 7d at the temperature of 35 ℃ to obtain the control non-antibiotic feed A2.
The candida tropicalis bacterial liquid prepared in the example 3 is concentrated at low temperature until the number of viable bacteria in the bacterial liquid is about 10 8/g, the bacterial liquid is added into the batch prepared in the example 4 according to the inoculation amount of 5%, and solid state fermentation is carried out for 7d at the temperature of 35 ℃, so that the control non-antibacterial feed A3 is obtained.
And (2) carrying out low-temperature concentration on the trichoderma longibrachiatum liquid, the aspergillus niger liquid and the candida tropicalis liquid obtained in the embodiments 1-3 until the number of live bacteria in the three kinds of liquid is about 10 8/g, mixing the three kinds of liquid according to the weight ratio of 1:1:3 to prepare a composite bacterial liquid, adding the composite bacterial liquid into the batch prepared in the embodiment 4 according to the inoculation amount of 5%, and carrying out solid state fermentation for 7d at 35 ℃ to obtain the non-antibiotic feed A4.
And (2) carrying out low-temperature concentration on the trichoderma longibrachiatum liquid, the aspergillus niger liquid and the candida tropicalis liquid obtained in the embodiments 1-3 until the number of live bacteria in the three kinds of liquid is about 10 8/g, mixing the three kinds of liquid according to the weight ratio of 3:1:1 to prepare a composite bacterial liquid, adding the composite bacterial liquid into the batch prepared in the embodiment 4 according to the inoculation amount of 5%, and carrying out solid state fermentation for 7d at 35 ℃ to obtain the non-antibiotic feed A5.
And (2) carrying out low-temperature concentration on the trichoderma longibrachiatum liquid, the aspergillus niger liquid and the candida tropicalis liquid obtained in the embodiments 1-3 to obtain three kinds of liquid, wherein the number of viable bacteria in the three kinds of liquid is about 10 8/g, mixing the three kinds of liquid according to the weight ratio of 1:3:1 to prepare a compound liquid, adding the compound liquid into the batch prepared in the embodiment 4 according to the inoculation amount of 5%, and carrying out solid state fermentation for 7d at the temperature of 35 ℃ to obtain the non-antibiotic feed A6.
Test example 1 application of the antibiotic-free feed of the invention in preventing and treating porcine diarrhea
In order to verify the application effect of the invention, the feed additive provided by the invention is tested in healthy piglets with similar weight. Pigs were divided into experimental groups (A1-A6) and control groups, 50 each. The daily ration of the experimental group is the non-antibiotic feed A1-A6 prepared in example 5, the daily ration of the control group is the mixed feed prepared in example 4 and having the same weight, each group of pigs are fed 3 times a day, the pigs are fed freely in the experimental period and provided with sufficient drinking water, other daily management measures are carried out according to the conventional management of a farm, immunization and insect repelling are carried out regularly, the number of escherichia coli in excrement is detected after 30 days, meanwhile, the morbidity of the pigs in the experimental group and the control group in the experimental period is counted, and the pig diarrhea rate of each group is calculated (see Table 1).
TABLE 1
| Number of Escherichia coli (10)7A/g) | Rate of diarrhea | |
| Control group | 5.72 | 26％ |
| Experimental group A1 | 3.68 | 18％ |
| Experimental group A2 | 3.57 | 18％ |
| Experimental group A3 | 3.19 | 16％ |
| Experimental group A4 | 2.66 | 10％ |
| Experimental group A5 | 2.07 | 8％ |
| Experimental group A6 | 1.34 | 2％ |
As can be seen from the comparative experiment of the test example 1, the antibiotic-free feed disclosed by the invention can obviously reduce the number of escherichia coli in bodies of piglets, reduce the chances of piglet infection diseases and reduce the morbidity and diarrhea rate of the piglets. In the test process, the piglet fed with the antibiotic-free feed is obviously better than a control group in mental state, and has the advantages of vigorous appetite, smooth fur and good color. In addition, under the condition that the total amount of the bacterial liquid is kept unchanged, the anti-pig diarrhea effect of the non-antibacterial feed prepared from the composite bacterial liquid prepared from the trichoderma longipedunculatum bacterial liquid, the aspergillus niger bacterial liquid and the candida tropicalis bacterial liquid is obviously better than that of a single bacterial liquid, wherein the effect of preventing and treating piglet diarrhea of the non-antibacterial feed prepared by mixing the trichoderma pedunculatum bacterial liquid, the aspergillus niger bacterial liquid and the candida tropicalis bacterial liquid in a weight ratio of 1:3:1 is most prominent, the piglet diarrhea rate is reduced to 2%, and the unexpected excellent effect is generated.
Claims (3)
1. The non-antibiotic feed for resisting the pig diarrhea is prepared by adding compound bacterial liquid into a batch mixture and performing solid state fermentation, and is characterized in that the compound bacterial liquid is prepared by compounding 1 part by weight of trichoderma longibrachiatum bacterial liquid, 3 parts by weight of aspergillus niger bacterial liquid and 1 part by weight of candida tropicalis bacterial liquid, and the batch mixture is prepared by uniformly mixing 40 parts by weight of soybean meal, 20 parts by weight of corn protein powder, 20 parts by weight of rapeseed meal, 6 parts by weight of peanut meal, 6 parts by weight of calcium hydrophosphate, 3 parts by weight of salt, 1 part by weight of lysine, 1 part by weight of methionine, 1 part by weight of threonine, 0.5 part by weight of vitamin A, 0.5 part by weight of vitamin C, 0.5 part by weight of vitamin E and 120.5 parts by weight of vitamin B.
2. The antibiotic-free feed of claim 1, wherein the weight ratio of the batch to the composite bacterial liquid is (90-95): (10-5).
3. The antibiotic-free feed of claim 2, wherein the weight ratio of the batch to the composite bacterial liquid is 95: 5.
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| CN103636921A (en) * | 2013-12-18 | 2014-03-19 | 海南椰国热带水果食品加工有限公司 | Microorganism feed additive and preparation method thereof |
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| CN1919044A (en) * | 2006-09-20 | 2007-02-28 | 南京林业大学 | Animal feedstuff additive and preparation method thereof |
| CN101401614A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Multi-bacterial fermentation production of protein feedstuff with steam exploration of vegetables dregs and cotton dregs and method thereof |
| CN103110033A (en) * | 2013-03-20 | 2013-05-22 | 邹平泰康生物饲料有限公司 | Pig feed additive and preparation method thereof |
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