CN106035414B - A kind of medicinal plant growth process agent and preparation method thereof and the application in promoting the accumulation of Wite red-rooted salvia root liposoluble constituent - Google Patents
A kind of medicinal plant growth process agent and preparation method thereof and the application in promoting the accumulation of Wite red-rooted salvia root liposoluble constituent Download PDFInfo
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Abstract
Application the invention discloses a kind of medicinal plant growth process agent and preparation method thereof and in promoting the accumulation of Wite red-rooted salvia root liposoluble constituent, the inorganic agent active ingredient are wood vinegar, boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae fibrous root leaching liquor and Chinese medicine extract, the wood vinegar be by obtained by yulan, Chinese scholar tree and yellow rose destructive distillation dry distillation liquid and water by 1:What 500 2000 volume ratio was obtained by mixing.Inorganic agent of the present invention is pollution-free, green, environmental-friendly, the use of wood vinegar is active ingredient, turns waste into wealth, of low cost.Inorganic agent preparation method of the present invention is simple, succinct quick, it is easy to accomplish, it is at low cost, it is convenient for industrialized production.Inorganic agent of the present invention can remarkably promote the content of liposoluble constituent in Wite red-rooted salvia root body, and high financial profit is of great significance to improving Radix Salviae Miltiorrhizae quality in production.
Description
Technical field
The present invention relates to a kind of medicinal plant growth process agent, the preparation method of the medicinal plant growth process agent is further related to
And promote the method for Wite red-rooted salvia root liposoluble constituent accumulation with it, belong to plant growth technology field.
Background technology
With being filled with for " back to nature " trend, traditional natural plant increasingly has been favored by people.Radix Salviae Miltiorrhizae source
In labiate Radix Salviae MiltiorrhizaeSalvia miltiorrhizaBunge. dry root has the work(such as promoting blood circulation and removing blood stasis, inducing meastruation to relieve menalgia
Effect is the important medicinal material for treating cardiovascular and cerebrovascular disease, belongs to China and commonly uses large Chinese medicine.Wite red-rooted salvia rootS. miltiorrhiza.
f. albaIt is the white flower modification of Radix Salviae Miltiorrhizae, treatment vasculitis effect is very good, causes extensive concern in the industry in recent years, and about such as
The effective substance content what is improved in Wite red-rooted salvia root body is rarely reported.It is domestic that Wite red-rooted salvia root is distributed mainly on Shandong, belongs to rare
Resources of medicinal plant is put into《Shandong Province's Chinese medicine standard》, Wite red-rooted salvia root liposoluble constituent is that one kind is widely present in rat-tail
Diterpene quinone in careless platymiscium is the main active of Wite red-rooted salvia root, including tanshinone (tanshinone
I), tanshinone IIA (tanshinone IIA), Tanshinone II B (tanshinone IIB) and Cryptotanshinone (
cryptanshinone) .Wite red-rooted salvia root liposoluble constituent be Radix Salviae Miltiorrhizae improve the heart, liver, lung ischemical reperfusion injury, tissue repair
Multiple regeneration adjusts immune response and anti-infective and other effects active principle basis.
Currently, market increasingly increases the demand of white flower red rooted salvia, Wite red-rooted salvia root production scale and cultivated area are not
It is disconnected to expand.As Wite red-rooted salvia root dosage increases year by year, medicinal herb grower covets yield and lavishment fertilizer and pesticide, leads to medicinal plant body
Interior effective substance content reduces and quality of medicinal material drastically declines.Therefore, adopt an effective measure fundamentally improve quality of medicinal material be
Ensure the effective way of this characteristic resources Sustainable Development and Utilization.
Invention content
The object of the present invention is to provide a kind of medicinal plant growth process agent, which is effectively taken by various composition
Match, the growth of Wite red-rooted salvia root and the accumulation of fat-soluble effective component, significant effect can be promoted.
It is a further object of the present invention to provide the preparation method of the inorganic agent, this method raw material sources are extensive, easy to operate,
It is easy to implement.
It is a further object of the present invention to provide application of the inorganic agent in promoting the accumulation of Wite red-rooted salvia root liposoluble constituent, use
Inorganic agent of the present invention handles Wite red-rooted salvia root plant, can significantly improve the content of liposoluble constituent in Wite red-rooted salvia root body, right
Radix Salviae Miltiorrhizae quality is improved in production to be of great significance.
Specific technical solution of the present invention is as follows：
A kind of medicinal plant growth process agent, the inorganic agent active ingredient are wood vinegar, boric acid, LaCl3, vitamin B, carboxylic
Methyl chitosan, Radix Salviae Miltiorrhizae fibrous root leaching liquor and Chinese medicine extract, the wood vinegar is will be obtained by yulan, Chinese scholar tree and yellow rose destructive distillation
Dry distillation liquid and water press 1:What the volume ratio of 500-2000 was obtained by mixing.
In above-mentioned medicinal plant growth process agent, the Radix Salviae Miltiorrhizae fibrous root leaching liquor is the water extract of Radix Salviae Miltiorrhizae fibrous root.Specifically
Preparation method be：It will be crushed after Radix Salviae Miltiorrhizae fibrous root drying at room temperature, cross 80 mesh sieve, be added according to every 0.5g Radix Salviae Miltiorrhizaes fibrous root crushed material
Distilled water is added in the proportioning of 1L distilled water, extracts for 24 hours, is filtered after extraction at room temperature, gained filtrate is Radix Salviae Miltiorrhizae fibrous root leaching liquor.
In above-mentioned medicinal plant growth process agent, the Chinese medicine extract can be the water decoction of rhizoma atractylodis and Chinese violet
Liquid.Wherein, rhizoma atractylodis and the mass ratio of Chinese violet are 1:2-5, preferably 1:3.
In above-mentioned medicinal plant growth process agent, the Chinese medicine extract can also be rhizoma atractylodis, Chinese violet, betel nut, half
The water decoction of branch lotus and frutus cnidii.Wherein, the mass ratio of rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and frutus cnidii is 1:2-5:
5-10:0.1-0.3:5-10, preferably 1:3:6:0.2:8.
Further, the preparation method of above-mentioned Chinese medicine extract is：Rhizoma atractylodis and Chinese violet are ground into fine powder, it is added
10 times of quality water decoct 2-2.5h and obtain；Either rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and frutus cnidii are ground into
Fine powder, the water that 10 times of their quality are added decoct 2-2.5h and obtain.
In above-mentioned medicinal plant growth process agent, the mass ratio of yulan, Chinese scholar tree and yellow rose is 2:2:3, use it
Stem and branch, realizing turns waste into wealth, at low cost, be convenient for industrialized production.
In above-mentioned medicinal plant growth process agent, mass concentration of the boric acid in wood vinegar is 5-15%, LaCl3In the wooden vinegar
Content in liquid is 2 × 10-4~10 × 10-4Mol/L, content of the vitamin B in wood vinegar are 0.5-5%, carboxymethyl chitosan
Content of the sugar in wood vinegar is 1-6%, and mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 0~5%, traditional Chinese medicine extraction
Mass concentration of the liquid in wood vinegar is 15-30%.
Preferably, mass concentration of the boric acid in wood vinegar is 5-15%, LaCl3Content in wood vinegar is 2 × 10-4-10×10-4Mol/L, content of the vitamin B in wood vinegar are 0.5-5%, content of the carboxymethyl chitosan in wood vinegar
For 1-6%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 1-5%, and quality of the Chinese medicine extract in wood vinegar is dense
Degree is 15-30%.
Preferably, mass concentration of the boric acid in wood vinegar is 10%, LaCl3Content in wood vinegar is 6.5 × 10-4
Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is 3%, the leaching of Radix Salviae Miltiorrhizae fibrous root
Mass concentration of the extract in wood vinegar is 3%, and mass concentration of the Chinese medicine extract in wood vinegar is 25%.
In above-mentioned medicinal plant growth process agent, the preparation method of wood vinegar is：Yulan, Chinese scholar tree and yellow rose are mixed,
Destructive distillation is carried out at 130~135 DEG C, 220~235 DEG C, 320~350 DEG C respectively, merges the destructive distillation of gained at a temperature of this three sections
Liquid cleans the dry distillation liquid after merging, then presses 1 with water:The volume ratio of 500-2000 mixes, and obtains wood vinegar.
Further, when preparing wood vinegar, at 130~135 DEG C keep the temperature 1.5h~2h, at 220~235 DEG C keep the temperature 2.5h~
3h keeps the temperature 1.5h~2h at 320~350 DEG C.
Further, when preparing wood vinegar, the dry distillation liquid after merging is cleaned using following methods：By the dry distillation liquid after merging
It stirs evenly, stratification, takes supernatant；Supernatant is distilled, gained distillate filters after being handled with activated carbon, takes filtrate.
Further, when preparing wood vinegar, supernatant is in 100~105 DEG C of air-distillation 2h；Its quality is added in distillate
10% activated carbon handles 45min at 95 DEG C.
The preparation method of the medicinal plant growth process agent of the present invention, includes the following steps：
（1）Yulan, Chinese scholar tree and yellow rose are done at 130~135 DEG C, 220~235 DEG C, 320~350 DEG C respectively
It evaporates, merges the dry distillation liquid of gained at a temperature of this three sections, the dry distillation liquid after merging is cleaned, then press 1 with water:The body of 500-2000
Product obtains wood vinegar than mixing；
（2）Traditional Chinese medicine powder is broken into fine powder, the water that 10 times of their gross masses are added decocts 2-2.5h, filtering, cooling, in obtaining
Medicament extracting solution；
（3）It will be crushed after Radix Salviae Miltiorrhizae fibrous root drying at room temperature, cross 80 mesh sieve, 1L is added according to every 0.5g Radix Salviae Miltiorrhizaes fibrous root crushed material
Distilled water is added in the proportioning of distilled water, extracts for 24 hours, is filtered after extraction at room temperature, gained filtrate is Radix Salviae Miltiorrhizae fibrous root leaching liquor；
（4）Boric acid, LaCl are added into wood vinegar3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae fibrous root leaching liquor and Chinese medicine
Extracting solution is uniformly mixed, obtains medicinal plant growth process agent.
The present invention also provides application of the inorganic agent in promoting the accumulation of Wite red-rooted salvia root liposoluble constituent, i.e., a kind of promotions
The method of Wite red-rooted salvia root liposoluble constituent accumulation, this method are：In Wite red-rooted salvia root incubation, above-mentioned medicinal of foliage spray
Plant growth inorganic agent, sprinkling is primary weekly, and being drenched to appear dripping with blade face is advisable.
The invention has the advantages that：
1, inorganic agent of the present invention is pollution-free, green, environmental-friendly, the use of wood vinegar is active ingredient, turns waste into wealth, cost
It is cheap.
2, inorganic agent preparation method of the present invention is simple, succinct quick, it is easy to accomplish, it is at low cost, it is convenient for industrialized production.
3, inorganic agent of the present invention can remarkably promote the content of liposoluble constituent in Wite red-rooted salvia root body, and high financial profit is right
Radix Salviae Miltiorrhizae quality is improved in production to be of great significance.
Specific implementation mode
Below by specific embodiment, the present invention will be further elaborated, it should be appreciated that, following the description is only
In order to explain the present invention, its content is not limited.
In following embodiments, Radix Salviae Miltiorrhizae fibrous root leaching liquor used is obtained in accordance with the following methods：By Radix Salviae Miltiorrhizae fibrous root in room
The lower drying of temperature, then crushes, crosses 80 mesh sieve；Precision weighs in appropriate crushed material to triangular pyramidal bottle, and distilled water is added（Often
1L distilled water is added in 0.5g crushed materials）, extract at 25 DEG C for 24 hours, vibrate in due course, filtered after micropore after filtered through gauze after extraction
Film, obtains filtrate, and gained filtrate is Radix Salviae Miltiorrhizae fibrous root leaching liquor.
Embodiment 1
Take stem, the branch position of yulan, Chinese scholar tree and yellow rose（Yulan：Chinese scholar tree：Yellow rose mass ratio 2:2:3）5kg is added dry
Kettle is evaporated, 130~135 DEG C is heated to, heat preservation 1.5h, continues to be heated to 220~235 DEG C, keep the temperature 2.5h, then temperature rises to 320
~350 DEG C of maintenance 1.5h.The dry distillation liquid of day part is collected, is mixed 30 minutes, is transferred in separatory funnel and stands 12 hours, point
Go out supernatant.Distillation still is added in supernatant, in 100~105 DEG C of air-distillations 2 hours, collects distillate.It is added in distillate
The activated carbon of quality 10% is heated with stirring to 95 DEG C, maintains 45 minutes, and filtered fluid is collected in filtering, by filtered fluid and water according to body
Product ratio 1:1000 ratio is uniformly mixed to get wood vinegar.
Embodiment 2
Take stem, the branch position of yulan, Chinese scholar tree and yellow rose（Yulan：Chinese scholar tree：Yellow rose mass ratio 2:2:3）5kg is added dry
Kettle is evaporated, 130~135 DEG C is heated to, heat preservation 1.5h, continues to be heated to 220~235 DEG C, keep the temperature 2.5h, then temperature rises to 320
~350 DEG C of maintenance 1.5h.The dry distillation liquid of day part is collected, is mixed 30 minutes, is transferred in separatory funnel and stands 12 hours, point
Go out supernatant.Distillation still is added in supernatant, in 100~105 DEG C of air-distillations 2 hours, collects distillate.It is added in distillate
The activated carbon of quality 10% is heated with stirring to 95 DEG C, maintains 45 minutes, and filtered fluid is collected in filtering, by filtered fluid and water according to body
Product ratio 1:500 ratio is uniformly mixed to get wood vinegar.
Embodiment 3
Take stem, the branch position of yulan, Chinese scholar tree and yellow rose（Yulan：Chinese scholar tree：Yellow rose mass ratio 2:2:3）5kg is added dry
Kettle is evaporated, 130~135 DEG C is heated to, heat preservation 1.5h, continues to be heated to 220~235 DEG C, keep the temperature 2.5h, then temperature rises to 320
~350 DEG C of maintenance 1.5h.The dry distillation liquid of day part is collected, is mixed 30 minutes, is transferred in separatory funnel and stands 12 hours, point
Go out supernatant.Distillation still is added in supernatant, in 100~105 DEG C of air-distillations 2 hours, collects distillate.It is added in distillate
The activated carbon of quality 10% is heated with stirring to 95 DEG C, maintains 45 minutes, and filtered fluid is collected in filtering, by filtered fluid and water according to body
Product ratio 1:2000 ratio is uniformly mixed to get wood vinegar.
Embodiment 4
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan and in
Medicament extracting solution, mass concentration of the boric acid in wood vinegar are 10%, LaCl3Content in wood vinegar is 6.5 × 10-4 mol/
L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is 3%, and Chinese medicine extract is in wood
Mass concentration in vinegar liquid is 25%, stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:3 mass ratio mixes rhizoma atractylodis and Chinese violet, crushes
At fine powder, the water of 10 times of rhizoma atractylodis and Chinese violet gross mass is then added, heating is decocted, and 2-2.5h is decocted, and gained decocts
Liquid filtering is cooled to room temperature, as Chinese medicine extract.
Embodiment 5
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan and in
Medicament extracting solution, mass concentration of the boric acid in wood vinegar are 5%, LaCl3Content in wood vinegar is 10 × 10-4Mol/L,
Content of the vitamin B in wood vinegar is 0.5%, and content of the carboxymethyl chitosan in wood vinegar is 6%, and Chinese medicine extract is in wood
Mass concentration in vinegar liquid is 15%, stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:3 mass ratio mixes rhizoma atractylodis and Chinese violet, crushes
At fine powder, the water of 10 times of rhizoma atractylodis and Chinese violet gross mass is then added, heating is decocted, and 2-2.5h is decocted, and gained decocts
Liquid filtering is cooled to room temperature, as Chinese medicine extract.
Embodiment 6
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan and in
Medicament extracting solution, it is 15%, LaCl to make mass concentration of the boric acid in wood vinegar3Content in wood vinegar is 2 × 10-4 mol/
L, content of the vitamin B in wood vinegar are 5%, and content of the carboxymethyl chitosan in wood vinegar is 1%, and Chinese medicine extract is in wood
Mass concentration in vinegar liquid is 30%, stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:3 mass ratio mixes rhizoma atractylodis rhizoma atractylodis and Chinese violet,
It is ground into fine powder, the water of 10 times of rhizoma atractylodis and Chinese violet gross mass is then added, heating is decocted, and 2-2.5h, gained are decocted
Decoction liquor filtering is cooled to room temperature, as Chinese medicine extract.
Embodiment 7
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan, pellet
Join fibrous root leaching liquor and Chinese medicine extract, it is 10%, LaCl to make mass concentration of the boric acid in wood vinegar3Containing in wood vinegar
Amount is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is
3%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 1%, and mass concentration of the Chinese medicine extract in wood vinegar is
25%, it stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:2 mass ratio mixes rhizoma atractylodis and Chinese violet, crushes
At fine powder, the water of 10 times of rhizoma atractylodis and Chinese violet gross mass is then added, heating is decocted, and 2-2.5h is decocted, and gained decocts
Liquid filtering is cooled to room temperature, as Chinese medicine extract.
Embodiment 8
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan, pellet
Join fibrous root leaching liquor and Chinese medicine extract, it is 10%, LaCl to make mass concentration of the boric acid in wood vinegar3Containing in wood vinegar
Amount is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is
3%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 5%, and mass concentration of the Chinese medicine extract in wood vinegar is
25%, it stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:5 mass ratio mixes rhizoma atractylodis and Chinese violet, crushes
At fine powder, the water of 10 times of rhizoma atractylodis and Chinese violet gross mass is then added, heating is decocted, and 2-2.5h is decocted, and gained decocts
Liquid filtering is cooled to room temperature, as Chinese medicine extract.
Embodiment 9
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan, pellet
Join fibrous root leaching liquor and Chinese medicine extract, it is 10%, LaCl to make mass concentration of the boric acid in wood vinegar3Containing in wood vinegar
Amount is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is
3%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 3%, and mass concentration of the Chinese medicine extract in wood vinegar is
25%, it stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:3:6:0.2:8 mass ratio is by rhizoma atractylodis, Chinese violet, Bin
Bulky, Sculellaria barbata and frutus cnidii mixing, are ground into fine powder, and it is total that rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and frutus cnidii is then added
The water that 10 times of quality, heating are decocted, and 2-2.5h is decocted, and the filtering of gained decoction liquor is cooled to room temperature, as Chinese medicine extract.
Embodiment 10
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan, pellet
Join fibrous root leaching liquor and Chinese medicine extract, it is 10%, LaCl to make mass concentration of the boric acid in wood vinegar3Containing in wood vinegar
Amount is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is
3%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 3%, and mass concentration of the Chinese medicine extract in wood vinegar is
25%, it stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:2:10:0.1:10 mass ratio by rhizoma atractylodis, Chinese violet,
Betel nut, Sculellaria barbata and frutus cnidii mixing, are ground into fine powder, rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and frutus cnidii are then added
The water that 10 times of gross mass, heating are decocted, and 2-2.5h is decocted, and the filtering of gained decoction liquor is cooled to room temperature, as traditional Chinese medicine extraction
Liquid.
Embodiment 11
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan, pellet
Join fibrous root leaching liquor and Chinese medicine extract, it is 10%, LaCl to make mass concentration of the boric acid in wood vinegar3Containing in wood vinegar
Amount is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is
3%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 3%, and mass concentration of the Chinese medicine extract in wood vinegar is
25%, it stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:5:5:0.3:5 mass ratio is by rhizoma atractylodis, Chinese violet, Bin
Bulky, Sculellaria barbata and frutus cnidii mixing, are ground into fine powder, and it is total that rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and frutus cnidii is then added
The water that 10 times of quality, heating are decocted, and 2-2.5h is decocted, and the filtering of gained decoction liquor is cooled to room temperature, as Chinese medicine extract.
Embodiment 12
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 2 thereto3, vitamin B, carboxymethyl chitosan, pellet
Join fibrous root leaching liquor and Chinese medicine extract, it is 10%, LaCl to make mass concentration of the boric acid in wood vinegar3Containing in wood vinegar
Amount is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is
3%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 3%, and mass concentration of the Chinese medicine extract in wood vinegar is
25%, it stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:3 mass ratio mixes rhizoma atractylodis and Chinese violet, crushes
At fine powder, the water of 10 times of rhizoma atractylodis and Chinese violet gross mass is then added, heating is decocted, and 2-2.5h is decocted, and gained decocts
Liquid filtering is cooled to room temperature, as Chinese medicine extract.
Embodiment 13
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 3 thereto3, vitamin B, carboxymethyl chitosan, pellet
Join fibrous root leaching liquor and Chinese medicine extract, it is 10%, LaCl to make mass concentration of the boric acid in wood vinegar3Containing in wood vinegar
Amount is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is
3%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 3%, and mass concentration of the Chinese medicine extract in wood vinegar is
25%, it stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:3:6:0.2:8 mass ratio is by rhizoma atractylodis, Chinese violet, Bin
Bulky, Sculellaria barbata and frutus cnidii mixing, are ground into fine powder, and it is total that rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and frutus cnidii is then added
The water that 10 times of quality, heating are decocted, and 2-2.5h is decocted, and the filtering of gained decoction liquor is cooled to room temperature, as Chinese medicine extract.
Embodiment 14
Boric acid, LaCl is added in the wood vinegar 1000ml of Example 1 thereto3, vitamin B, carboxymethyl chitosan, pellet
Join fibrous root leaching liquor and Chinese medicine extract, it is 10%, LaCl to make mass concentration of the boric acid in wood vinegar3Containing in wood vinegar
Amount is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%, and content of the carboxymethyl chitosan in wood vinegar is
3%, mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 3%, and mass concentration of the Chinese medicine extract in wood vinegar is
25%, it stirs evenly, obtains medicinal plant growth process agent.
Wherein, the preparation method of Chinese medicine extract is：According to 1:3 mass ratio mixes rhizoma atractylodis and Chinese violet, crushes
At fine powder, the water of 10 times of rhizoma atractylodis and Chinese violet gross mass is then added, heating is decocted, and 2-2.5h is decocted, and gained decocts
Liquid filtering is cooled to room temperature, as Chinese medicine extract.
Embodiment 15
(1) plant culture and processing
Material to be tested is Wite red-rooted salvia rootS. miltiorrhiza. f. albaSeed source is in the planting bases Radix Salviae Miltiorrhizae GAP.
In the same size, healthy salvia seeds are chosen, are seeded in gardens soil and the sandy soil of river sand mixing（1:1, v/v）, 25 ± 1 DEG C
It sprouts.After third piece blade is grown（Seedling age about 50d）, choose the consistent Radix Salviae Miltiorrhizae seedling replanting of growing way and enter equipped with above-mentioned sandy soil
Square basin（Per basin sand weight 500g, diameter 10cm, per 1 plant of basin）It is interior, it is placed in artificial climate room, condition of culture：28℃/22℃
（Day night）, 14 h of daily illumination, 280-300 μm of m of light intensity-2s-1, relative humidity 60%-65%.The consistent seedling of growing way is chosen,
Radix Salviae Miltiorrhizae blade is sprayed with the treatment fluid described in table 2 respectively, sprays and is uniformly covered with drop and not lower drop with leave dual sides
It is limited, processing in every 30 days is primary, and 150d carries out the fat-soluble detection being divided into biomass and red sage root after processing.
(2) promote plant strain growth to measure
Plant strain growth is promoted to be indicated using under ground portion fresh weight and dry weight and plant height, with distilled water by white flower when harvest
The moisture for being attached to Wite red-rooted salvia root root surface is rinsed and blotted with blotting paper to the root surface of Radix Salviae Miltiorrhizae well, weighs fresh weight.It is then low
Drying to constant weight weighs the dry weight of root for temperature.Meanwhile measuring the plant height of Wite red-rooted salvia root aerial part plant.
(3) liposoluble constituent measures
Red sage root and rhizome are cleaned into rear, drying to permanent quality, ground into the fine powder that can be sieved by 380 μm, mixing claims matter
Amount.The ratio that 1 mL methanol is added according to 0.1g dry mass is added the methanol of corresponding amount, ultrasonic extraction 45min, it is cooling after from
The heart takes supernatant to cross 0.45 μm of miillpore filter, spare.The content of tanshinone is analyzed with high performance liquid chromatography (HPLC),
HPLC system is by 1525 binary high performance liquid chromatographs of Waters, 2487 detectors of Waters, Waters Sun Fire
C18 chromatographic columns (250mm × 4.6mm, 5 μm), Empower2 chromatography softwares composition.With acetonitrile（A liquid）And volume fraction
0.01% phosphate aqueous solution（B liquid）（pH=2.6）For mobile phase, using binary gradient elutes (table 1).Detection wavelength is 270 nm,
Flow velocity 1.0m L/min, 30 DEG C of column temperature.Each sample repeats 3 times, and the tanshinone predominantly detected is Cryptotanshinone, tanshinone
I and tanshinone IIA detect the tanshinone standard items sample used and are bought from Nat'l Pharmaceutical ＆ Biological Products Control Institute (Beijing).
（4）Test result
The Salvia miltiorrhiza Growth situation and liposoluble constituent content that are handled using different disposal liquid are detected, as a result such as
Shown in the following table 2.As can be seen from the table, plant growth inorganic agent of the present invention can promote Wite red-rooted salvia root to grow, and can also improve its fat
Soluble components content, the growth and liposoluble constituent content of the variation of plant growth inorganic agent ingredient and content to Wite red-rooted salvia root
Have an impact.Wherein, the inorganic agent best results of embodiment 9.In addition, being compared by embodiment 1 and the experimental result of distilled water
As can be seen that being applied alone wood vinegar to Wite red-rooted salvia root growth promotion and liposoluble constituent accumulation effect unobvious.
Claims (6)
1. a kind of medicinal plant growth process agent, it is characterized in that：Active ingredient is wood vinegar, boric acid, LaCl3, vitamin B, carboxylic first
Base enclosure glycan, Radix Salviae Miltiorrhizae fibrous root leaching liquor and Chinese medicine extract, the wood vinegar is will be obtained by yulan, Chinese scholar tree and yellow rose destructive distillation
Dry distillation liquid presses 1 with water:What the volume ratio of 500-2000 was obtained by mixing；The Chinese medicine extract is the decocting of rhizoma atractylodis and Chinese violet
Boil liquid or rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and frutus cnidii water decoction；The Radix Salviae Miltiorrhizae fibrous root leaching liquor is pellet
The mass ratio of the water extract of ginseng fibrous root, rhizoma atractylodis and Chinese violet is 1:2-5；Rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and snake
The mass ratio of machine tool is 1:2-5:5-10:0.1-0.3:5-10；
Mass concentration of the boric acid in wood vinegar is 5-15%, LaCl3Content in wood vinegar is 2 × 10-4~10 × 10-4
Mol/L, content of the vitamin B in wood vinegar are 0.5-5%, and content of the carboxymethyl chitosan in wood vinegar is 1-6%, Radix Salviae Miltiorrhizae
Mass concentration of the fibrous root leaching liquor in wood vinegar is 0~5%, and mass concentration of the Chinese medicine extract in wood vinegar is 15-
30%；The mass ratio of yulan, Chinese scholar tree and yellow rose is 2:2:3.
2. medicinal plant growth process agent according to claim 1, it is characterized in that：Rhizoma atractylodis and the mass ratio of Chinese violet are
1:3；Rhizoma atractylodis, Chinese violet, betel nut, Sculellaria barbata and frutus cnidii mass ratio be 1:3:6:0.2:8.
3. medicinal plant growth process agent according to claim 1 or 2, it is characterized in that：Quality of the boric acid in wood vinegar
A concentration of 10%, LaCl3Content in wood vinegar is 6.5 × 10-4Mol/L, content of the vitamin B in wood vinegar are 2%,
Content of the carboxymethyl chitosan in wood vinegar is 3%, and mass concentration of the Radix Salviae Miltiorrhizae fibrous root leaching liquor in wood vinegar is 3%, Chinese medicine
Mass concentration of the extracting solution in wood vinegar is 25%.
4. medicinal plant growth process agent according to claim 1 or 2, it is characterized in that：The preparation method of wood vinegar is：It will
Yulan, Chinese scholar tree and yellow rose mixing, carry out destructive distillation at 130~135 DEG C, 220~235 DEG C, 320~350 DEG C respectively, merge this
The dry distillation liquid of gained at a temperature of three sections, the dry distillation liquid after merging is cleaned, and then presses 1 with water:The volume ratio of 500-2000 mixes,
Obtain wood vinegar.
5. the preparation method of the medicinal plant growth process agent described in a kind of any one of claim 1-4, it is characterized in that including
Following steps：
（1）Yulan, Chinese scholar tree and yellow rose are subjected to destructive distillation at 130~135 DEG C, 220~235 DEG C, 320~350 DEG C respectively, closed
And at a temperature of this three sections then the dry distillation liquid removal of impurities after merging is pressed 1 by the dry distillation liquid of gained with water:The volume ratio of 500-2000
Mixing, obtains wood vinegar；
（2）Traditional Chinese medicine powder is broken into fine powder, the water that 10 times of their gross masses are added decocts 2-2.5h, and filtering, cooling obtain Chinese medicine and carry
Take liquid；
（3）It will be crushed after Radix Salviae Miltiorrhizae fibrous root drying at room temperature, cross 80 mesh sieve, 1L distillations are added according to every 0.5g Radix Salviae Miltiorrhizaes fibrous root crushed material
Distilled water is added in the proportioning of water, extracts for 24 hours, is filtered after extraction at room temperature, gained filtrate is Radix Salviae Miltiorrhizae fibrous root leaching liquor；
（4）Boric acid, LaCl are added into wood vinegar3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae fibrous root leaching liquor and traditional Chinese medicine extraction
Liquid is uniformly mixed, obtains medicinal plant growth process agent.
6. a kind of method promoting the accumulation of Wite red-rooted salvia root liposoluble constituent, it is characterized in that：In Wite red-rooted salvia root incubation, blade face
The medicinal plant growth process agent described in any one of claim 1-4 is sprayed, sprinkling in every 30 days is primary, equal with leave dual sides
It is even to be covered with drop and do not descend drop not to be limited.
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| CN102649654A (en) * | 2012-04-25 | 2012-08-29 | 孟凡尧 | Traditional Chinese medicine organic fertilizer |
| CN104982464A (en) * | 2015-06-26 | 2015-10-21 | 安徽禾众农业科技有限公司 | Bacteriostatic agent for preventing and treating strawberry gray mold and preparation method and application thereof |
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| CN104982464A (en) * | 2015-06-26 | 2015-10-21 | 安徽禾众农业科技有限公司 | Bacteriostatic agent for preventing and treating strawberry gray mold and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| "Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures";Jiang-Lin Zhao等;《Appl Microbiol Biotechnol》;20100630;第87卷(第1期);第140页倒数第2段和fig2 * |
| "Effects of LaCl3 on photosynthesis and the accumulation of tanshinones and salvianolic acids in Salvia miltiorrhiza seedlings";ZHOU Jie. etal;《JOURNAL OF RARE EARTHS》;20110531;第29卷(第5期);第497页第4段和fig4 * |
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