CN106035414A - Growth treating agent for medicinal plants, preparation method of growth treating agent and application of growth treating agent in promoting accumulation of Salvia miltiorrhiza Bunge var. alba lipid-soluble ingredients - Google Patents
Growth treating agent for medicinal plants, preparation method of growth treating agent and application of growth treating agent in promoting accumulation of Salvia miltiorrhiza Bunge var. alba lipid-soluble ingredients Download PDFInfo
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Abstract
The invention discloses a growth treating agent for medicinal plants, a preparation method of the growth treating agent and application of the growth treating agent in promoting accumulation of Salvia miltiorrhiza Bunge var. alba lipid-soluble ingredients. The treating agent includes the effective ingredients: wood vinegar, boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Salvia miltiorrhiza fibril extract and a traditional Chinese medicine extract; the wood vinegar is made by mixing water with dry distillate produced by dry distilling Magnolia denudate, Sophora japonica and Rosa xanthina according to a volume ratio of 1:500-2000. The treating agent is pollution-free, green and environment-friendly and uses the wood vinegar as an effective ingredient, turning waste into wealth and enabling low cost. The preparation method of the treating agent of the invention is simple, simple and fast, easy to implement, low in cost and facilitates industrial production; the treating agent can significantly promote the content of lipid-soluble ingredients in Salvia miltiorrhiza Bunge var. alba, provides high economic benefit and is significant to improving Salvia miltiorrhiza quality in terms of production.
Description
Technical field
The present invention relates to a kind of medicinal plants growth process agent, further relate to the preparation method of this medicinal plants growth process agent
And the method with its promotion Salvia miltiorrhiza f. liposoluble constituent accumulation, belong to plant growth technology field.
Background technology
Along with being filled with of " back to nature " trend, tradition natural plant is increasingly favored by people.Radix Salviae Miltiorrhizae is originated
In labiate Radix Salviae MiltiorrhizaeSalvia miltiorrhizaBunge. dry root, has the merit such as blood circulation promoting and blood stasis dispelling, inducing menstruation to relieve menalgia
Effect, is the important medical material for the treatment of cardiovascular and cerebrovascular disease, belongs to China and commonly uses large Chinese crude drug.Salvia miltiorrhiza f.S. miltiorrhiza.
f. albaBeing the modification of spending in vain of Radix Salviae Miltiorrhizae, treatment vasculitis effect is the best, causes extensive concern in the industry in recent years, and about such as
The effective substance content what improves in Salvia miltiorrhiza f. body rarely has report.It is domestic that Salvia miltiorrhiza f. is distributed mainly on Shandong, belongs to rare
Resources of medicinal plant, is put into " Shandong Province's Chinese crude drug standard ", and Salvia miltiorrhiza f. liposoluble constituent is that a class is widely present in rat-tail
Diterpene quinone in grass platymiscium, is the main active of Salvia miltiorrhiza f., including TANSHINONES (tanshinone
I), tanshinone ⅡA (tanshinone IIA), Tanshinone II B (tanshinone IIB) and cryptotanshinone (
cryptanshinone) .Salvia miltiorrhiza f. liposoluble constituent is that Radix Salviae Miltiorrhizae improves the heart, liver, the ischemical reperfusion injury of lung, organizes and repair
Multiple regeneration, the active substance basis of regulation effect such as immunne response and infection.
At present, the demand spending red rooted salvia in vain is increased by market day by day, and Salvia miltiorrhiza f. production scale and cultivated area are not
Disconnected expansion.Along with Salvia miltiorrhiza f. consumption increases year by year, medicinal herb grower covets yield and lavishment fertilizer and pesticide, causes medicinal plants body
Interior effective substance content reduces and quality of medicinal material drastically declines.Therefore, fundamentally raising quality of medicinal material of adopting an effective measure is
Ensure the effective way of this characteristic resources Sustainable Development and Utilization.
Summary of the invention
It is an object of the invention to provide a kind of medicinal plants growth process agent, the effective collocation by various compositions of this inorganic agent,
Can promote growth and the accumulation of fat-soluble active ingredient of Salvia miltiorrhiza f., effect is notable.
It is a further object of the present invention to provide the preparation method of this inorganic agent, the method raw material sources are extensive, easy to operate,
Easy to implement.
It is a further object of the present invention to provide the application in promoting the accumulation of Salvia miltiorrhiza f. liposoluble constituent of this inorganic agent, use
Salvia miltiorrhiza f. plant is processed by inorganic agent of the present invention, can significantly improve the content of liposoluble constituent in Salvia miltiorrhiza f. body, right
Radix Salviae Miltiorrhizae quality is improved significant in production.
The concrete technical scheme of the present invention is as follows:
A kind of medicinal plants growth process agent, this inorganic agent effective ingredient is wood vinegar, boric acid, LaCl3, vitamin B, carboxymethyl
Chitosan, Radix Salviae Miltiorrhizae fibrous root lixiviating solution and Chinese medicine extraction liquid, described wood vinegar is doing Magnolia denudata, Chinese scholar tree and yellow rose dry distilling gained
Distillate and water are obtained by mixing by the volume ratio of 1:500-2000.
In above-mentioned medicinal plants growth process agent, described Radix Salviae Miltiorrhizae fibrous root lixiviating solution is the water extract of Radix Salviae Miltiorrhizae fibrous root.Specifically
Preparation method be: will after Radix Salviae Miltiorrhizae fibrous root drying at room temperature pulverize, cross 80 mesh sieves, according to every 0.5g Radix Salviae Miltiorrhizae fibrous root ground product add
The proportioning of 1L distilled water adds distilled water, extracts 24h, filter after extraction under room temperature, and gained filtrate is Radix Salviae Miltiorrhizae fibrous root lixiviating solution.
In above-mentioned medicinal plants growth process agent, described Chinese medicine extraction liquid can be the soak by water of Rhizoma Atractylodis and Herba Violae
Liquid.Wherein, the mass ratio of Rhizoma Atractylodis and Herba Violae is 1:2-5, preferably 1:3.
In above-mentioned medicinal plants growth process agent, described Chinese medicine extraction liquid can also is that Rhizoma Atractylodis, Herba Violae, Semen Arecae, half
Branch lotus and the water decoction of Fructus Cnidii.Wherein, the mass ratio of Rhizoma Atractylodis, Herba Violae, Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii is 1:2-5:
5-10:0.1-0.3:5-10, preferably 1:3:6:0.2:8.
Further, the preparation method of above-mentioned Chinese medicine extraction liquid is: Rhizoma Atractylodis and Herba Violae are ground into fine powder, add it
The soak by water 2-2.5h of quality 10 times and obtain；Or Rhizoma Atractylodis, Herba Violae, Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii are ground into
Fine powder, adds the soak by water 2-2.5h of they quality 10 times and obtains.
In above-mentioned medicinal plants growth process agent, the mass ratio of Magnolia denudata, Chinese scholar tree and yellow rose is 2:2:3, uses it
Stem and branch, it is achieved that turn waste into wealth, low cost, it is simple to industrialized production.
In above-mentioned medicinal plants growth process agent, boric acid mass concentration in wood vinegar is 5-15%, LaCl3At wood vinegar
Content in liquid is 2 × 10-4～10 × 10-4Mol/L, vitamin B content in wood vinegar is 0.5-5%, carboxymethyl chitosan
Sugar content in wood vinegar is 1-6%, and Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 0～5%, Chinese medicine extraction
Liquid mass concentration in wood vinegar is 15-30%.
Preferably, boric acid mass concentration in wood vinegar is 5-15%, LaCl3Content in wood vinegar is 2 × 10-4-10×10-4Mol/L, vitamin B content in wood vinegar is 0.5-5%, carboxymethyl chitosan content in wood vinegar
For 1-6%, Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 1-5%, and Chinese medicine extraction liquid quality in wood vinegar is dense
Degree is 15-30%.
Preferably, boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is 6.5 × 10-4
Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%, and Radix Salviae Miltiorrhizae fibrous root soaks
Extract mass concentration in wood vinegar is 3%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%.
In above-mentioned medicinal plants growth process agent, the preparation method of wood vinegar is: Magnolia denudata, Chinese scholar tree and yellow rose are mixed,
Respectively 130～135 DEG C, 220～235 DEG C, carry out dry distilling at 320～350 DEG C, merge the dry distilling of gained at a temperature of these three sections
Liquid, the dry distillation liquid remove impurity after merging, then mix by the volume ratio of 1:500-2000 with water, obtain wood vinegar.
Further, when preparing wood vinegar, 130～135 DEG C be incubated 1.5h～2h, 220～235 DEG C insulation 2.5h～
3h, is incubated 1.5h～2h at 320～350 DEG C.
Further, when preparing wood vinegar, the dry distillation liquid after merging uses following method remove impurity: the dry distillation liquid after merging stirs
Uniformly, stratification, take supernatant；Being distilled by supernatant, gained distillate filters after processing with activated carbon, takes filtrate.
Further, when preparing wood vinegar, supernatant is at 100～105 DEG C of air-distillation 2h；Distillate adds its quality
The activated carbon of 10% processes 45min at 95 DEG C.
The preparation method of the medicinal plants growth process agent of the present invention, comprises the following steps:
(1) by Magnolia denudata, Chinese scholar tree and yellow rose respectively 130～135 DEG C, 220～235 DEG C, carry out dry distilling at 320～350 DEG C, close
And the dry distillation liquid of gained at a temperature of these three sections, the dry distillation liquid remove impurity after merging, the volume ratio of 1:500-2000 is then pressed with water
Mixing, obtains wood vinegar；
(2) traditional Chinese medicine powder is broken into fine powder, adds the soak by water 2-2.5h of they gross masses 10 times, filter, cool down, obtain Chinese medicine and carry
Take liquid；
(3) will pulverize after Radix Salviae Miltiorrhizae fibrous root drying at room temperature, cross 80 mesh sieves, add 1L distillation according to every 0.5g Radix Salviae Miltiorrhizae fibrous root ground product
The proportioning of water adds distilled water, extracts 24h, filter after extraction under room temperature, and gained filtrate is Radix Salviae Miltiorrhizae fibrous root lixiviating solution；
(4) in wood vinegar, boric acid, LaCl are added3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae fibrous root lixiviating solution and Chinese medicine extraction
Liquid, mix homogeneously, obtain medicinal plants growth process agent.
Present invention also offers the application in promoting the accumulation of Salvia miltiorrhiza f. liposoluble constituent of this inorganic agent, i.e. a kind of promotion
The method of Salvia miltiorrhiza f. liposoluble constituent accumulation, the method is: in Salvia miltiorrhiza f. incubation, above-mentioned medicinal of foliage spray
Plant growing inorganic agent, sprays weekly once, is drenched to appear dripping with blade face and is advisable.
The method have the advantages that
1, inorganic agent of the present invention is pollution-free, and green, environmental friendliness, use wood vinegar is effective ingredient, turns waste into wealth, low cost
Honest and clean.
2, inorganic agent preparation method of the present invention is simple, the most quickly, it is easy to accomplish, low cost, it is simple to industrialized production.
3, the content of liposoluble constituent in inorganic agent of the present invention can remarkably promote Salvia miltiorrhiza f. body, high financial profit, right
Radix Salviae Miltiorrhizae quality is improved significant in production.
Detailed description of the invention
Below by specific embodiment, the present invention will be further elaborated, it should be appreciated that, the description below is only
In order to explain the present invention, its content is not limited.
In following embodiment, Radix Salviae Miltiorrhizae fibrous root lixiviating solution used prepares in accordance with the following methods: by Radix Salviae Miltiorrhizae fibrous root in room
Temperature is lower to be dried, and then pulverizes, crosses 80 mesh sieves；Precision weighs appropriate ground product in triangular pyramidal bottle, adds distilled water (every
0.5g ground product adds 1L distilled water), at 25 DEG C, extract 24h, vibrate in good time, filter after micropore after filtered through gauze after extraction
Film, obtains filtrate, and gained filtrate is Radix Salviae Miltiorrhizae fibrous root lixiviating solution.
Embodiment 1
Take the stem of Magnolia denudata, Chinese scholar tree and yellow rose, branch position (Magnolia denudata: Chinese scholar tree: yellow rose mass ratio 2:2:3) 5kg, add dry distilling
Still, is heated to 130～135 DEG C, insulation 1.5h, continue to be heated to 220～235 DEG C, be incubated 2.5h, then temperature rise to 320～
350 DEG C maintain 1.5h.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed to separatory funnel stands 12 hours, separate
Supernatant.Supernatant is added distillating still, in 100～105 DEG C of air-distillations 2 hours, collects distillate.Distillate adds its matter
The activated carbon of amount 10%, is heated with stirring to 95 DEG C, maintains 45 minutes, filters, and collects filtrate, by filtrate with water according to volume
Ratio mix homogeneously than 1:1000, obtains wood vinegar.
Embodiment 2
Take the stem of Magnolia denudata, Chinese scholar tree and yellow rose, branch position (Magnolia denudata: Chinese scholar tree: yellow rose mass ratio 2:2:3) 5kg, add dry distilling
Still, is heated to 130～135 DEG C, insulation 1.5h, continue to be heated to 220～235 DEG C, be incubated 2.5h, then temperature rise to 320～
350 DEG C maintain 1.5h.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed to separatory funnel stands 12 hours, separate
Supernatant.Supernatant is added distillating still, in 100～105 DEG C of air-distillations 2 hours, collects distillate.Distillate adds its matter
The activated carbon of amount 10%, is heated with stirring to 95 DEG C, maintains 45 minutes, filters, and collects filtrate, by filtrate with water according to volume
Ratio mix homogeneously than 1:500, obtains wood vinegar.
Embodiment 3
Take the stem of Magnolia denudata, Chinese scholar tree and yellow rose, branch position (Magnolia denudata: Chinese scholar tree: yellow rose mass ratio 2:2:3) 5kg, add dry distilling
Still, is heated to 130～135 DEG C, insulation 1.5h, continue to be heated to 220～235 DEG C, be incubated 2.5h, then temperature rise to 320～
350 DEG C maintain 1.5h.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed to separatory funnel stands 12 hours, separate
Supernatant.Supernatant is added distillating still, in 100～105 DEG C of air-distillations 2 hours, collects distillate.Distillate adds its matter
The activated carbon of amount 10%, is heated with stirring to 95 DEG C, maintains 45 minutes, filters, and collects filtrate, by filtrate with water according to volume
Ratio mix homogeneously than 1:2000, obtains wood vinegar.
Embodiment 4
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan and Chinese medicine carries
Taking liquid, boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is 6.5 × 10-4Mol/L, dimension
Raw element B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%, and Chinese medicine extraction liquid is at wood vinegar
In mass concentration be 25%, stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: Rhizoma Atractylodis and Herba Violae is mixed according to the mass ratio of 1:3, pulverizes
Becoming fine powder, be subsequently adding Rhizoma Atractylodis and the water of Herba Violae gross mass 10 times, heating decocts, and decocts 2-2.5h, and gained decocts
Liquid filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 5
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan and Chinese medicine carries
Taking liquid, boric acid mass concentration in wood vinegar is 5%, LaCl3Content in wood vinegar is 10 × 10-4Mol/L, dimension is raw
Element B content in wood vinegar is 0.5%, and carboxymethyl chitosan content in wood vinegar is 6%, and Chinese medicine extraction liquid is at wood vinegar
In mass concentration be 15%, stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: Rhizoma Atractylodis and Herba Violae is mixed according to the mass ratio of 1:3, pulverizes
Becoming fine powder, be subsequently adding Rhizoma Atractylodis and the water of Herba Violae gross mass 10 times, heating decocts, and decocts 2-2.5h, and gained decocts
Liquid filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 6
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan and Chinese medicine carries
Taking liquid, making boric acid mass concentration in wood vinegar is 15%, LaCl3Content in wood vinegar is 2 × 10-4Mol/L, dimension
Raw element B content in wood vinegar is 5%, and carboxymethyl chitosan content in wood vinegar is 1%, and Chinese medicine extraction liquid is at wood vinegar
In mass concentration be 30%, stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: Rhizoma Atractylodis Rhizoma Atractylodis and Herba Violae are mixed according to the mass ratio of 1:3,
Being ground into fine powder, be subsequently adding Rhizoma Atractylodis and the water of Herba Violae gross mass 10 times, heating decocts, and decocts 2-2.5h, gained
Decoction liquor filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 7
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae palpus
Root lixiviating solution and Chinese medicine extraction liquid, making boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is
6.5×10-4Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%,
Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 1%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%,
Stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: Rhizoma Atractylodis and Herba Violae is mixed according to the mass ratio of 1:2, pulverizes
Becoming fine powder, be subsequently adding Rhizoma Atractylodis and the water of Herba Violae gross mass 10 times, heating decocts, and decocts 2-2.5h, and gained decocts
Liquid filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 8
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae palpus
Root lixiviating solution and Chinese medicine extraction liquid, making boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is
6.5×10-4Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%,
Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 5%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%,
Stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: Rhizoma Atractylodis and Herba Violae is mixed according to the mass ratio of 1:5, pulverizes
Becoming fine powder, be subsequently adding Rhizoma Atractylodis and the water of Herba Violae gross mass 10 times, heating decocts, and decocts 2-2.5h, and gained decocts
Liquid filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 9
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae palpus
Root lixiviating solution and Chinese medicine extraction liquid, making boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is
6.5×10-4Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%,
Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 3%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%,
Stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: according to the mass ratio of 1:3:6:0.2:8 by Rhizoma Atractylodis, Herba Violae, Bin
Bulky, Herba Scutellariae Barbatae and Fructus Cnidii mixing, be ground into fine powder, be subsequently adding Rhizoma Atractylodis, Herba Violae, Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii total
The water that quality is 10 times, heating decocts, and decocts 2-2.5h, and gained decoction liquor filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 10
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae palpus
Root lixiviating solution and Chinese medicine extraction liquid, making boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is
6.5×10-4Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%,
Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 3%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%,
Stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: according to 1:2:10:0.1:10 mass ratio by Rhizoma Atractylodis, Herba Violae,
Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii mixing, be ground into fine powder, be subsequently adding Rhizoma Atractylodis, Herba Violae, Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii
The water that gross mass is 10 times, heating decocts, and decocts 2-2.5h, and gained decoction liquor filters, is cooled to room temperature, is Chinese medicine extraction
Liquid.
Embodiment 11
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae palpus
Root lixiviating solution and Chinese medicine extraction liquid, making boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is
6.5×10-4Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%,
Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 3%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%,
Stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: according to the mass ratio of 1:5:5:0.3:5 by Rhizoma Atractylodis, Herba Violae, Bin
Bulky, Herba Scutellariae Barbatae and Fructus Cnidii mixing, be ground into fine powder, be subsequently adding Rhizoma Atractylodis, Herba Violae, Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii total
The water that quality is 10 times, heating decocts, and decocts 2-2.5h, and gained decoction liquor filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 12
The wood vinegar 1000ml of Example 2, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae palpus
Root lixiviating solution and Chinese medicine extraction liquid, making boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is
6.5×10-4Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%,
Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 3%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%,
Stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: Rhizoma Atractylodis and Herba Violae is mixed according to the mass ratio of 1:3, pulverizes
Becoming fine powder, be subsequently adding Rhizoma Atractylodis and the water of Herba Violae gross mass 10 times, heating decocts, and decocts 2-2.5h, and gained decocts
Liquid filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 13
The wood vinegar 1000ml of Example 3, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae palpus
Root lixiviating solution and Chinese medicine extraction liquid, making boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is
6.5×10-4Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%,
Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 3%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%,
Stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: according to the mass ratio of 1:3:6:0.2:8 by Rhizoma Atractylodis, Herba Violae, Bin
Bulky, Herba Scutellariae Barbatae and Fructus Cnidii mixing, be ground into fine powder, be subsequently adding Rhizoma Atractylodis, Herba Violae, Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii total
The water that quality is 10 times, heating decocts, and decocts 2-2.5h, and gained decoction liquor filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 14
The wood vinegar 1000ml of Example 1, is added thereto to boric acid, LaCl3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae palpus
Root lixiviating solution and Chinese medicine extraction liquid, making boric acid mass concentration in wood vinegar is 10%, LaCl3Content in wood vinegar is
6.5×10-4Mol/L, vitamin B content in wood vinegar is 2%, and carboxymethyl chitosan content in wood vinegar is 3%,
Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is 3%, and Chinese medicine extraction liquid mass concentration in wood vinegar is 25%,
Stir, obtain medicinal plants growth process agent.
Wherein, the preparation method of Chinese medicine extraction liquid is: Rhizoma Atractylodis and Herba Violae is mixed according to the mass ratio of 1:3, pulverizes
Becoming fine powder, be subsequently adding Rhizoma Atractylodis and the water of Herba Violae gross mass 10 times, heating decocts, and decocts 2-2.5h, and gained decocts
Liquid filters, is cooled to room temperature, is Chinese medicine extraction liquid.
Embodiment 15
(1) plant culturing and process
Material to be tested is Salvia miltiorrhiza f.S. miltiorrhiza. f. albaSeed source is in Radix Salviae Miltiorrhizae GAP planting base.Choose
In the same size, healthy salvia seeds, is seeded in the sandy soil of gardens soil and river sand mixing (1:1, v/v), 25 ± 1 DEG C of sproutings.
After the 3rd blade grows (seedling age about 50d), choose the consistent Radix Salviae Miltiorrhizae seedling replanting of growing way and enter the square basin equipped with above-mentioned sandy soil
In (every basin sand weight 500g, diameter 10cm, the strain of every basin 1), it is placed in artificial climate indoor, condition of culture: 28 DEG C/22 DEG C (daytime/
Night), illumination every day 14 h, light intensity 280-300 μm m-2s-1, relative humidity 60%-65%.Choose the seedling that growing way is consistent, respectively
With the treatment fluid described in table 2, Radix Salviae Miltiorrhizae blade is sprayed, spray uniformly be covered with drop with leave dual sides and the most lower be limited,
Within every 30 days, process once, 150d fat-soluble detection being divided in carrying out Biomass and red sage root after process.
(2) promote that plant strain growth measures
Promote that plant strain growth uses under ground portion fresh weight and dry weight and plant height to represent, with distilled water by Salvia miltiorrhiza f. during results
Root surface rinse well and blot the moisture being attached to Salvia miltiorrhiza f. root surface with absorbent paper, weigh fresh weight.Then low temperature dries
Do to constant weight and weigh the dry weight of root.Meanwhile, the plant height of Salvia miltiorrhiza f. aerial parts plant is measured.
(3) liposoluble constituent measures
After red sage root and rhizome are cleaned, it is dried to permanent quality, grinds into and can claim quality by the fine powder of 380 m sieves, mixing.
Add the methanol of the ratio addition respective amount of 1 mL methanol, supersound extraction 45min according to 0.1g dry mass, be centrifuged after cooling, take
Supernatant crosses 0.45 m microporous filter membrane, standby.The content high performance liquid chromatography (HPLC) of TANSHINONES is analyzed, HPLC system
System is by Waters 1525 binary high performance liquid chromatograph, Waters 2487 detector, Waters Sun Fire C18 chromatograph
Post (250mm × 4.6mm, 5 m), Empower2 chromatography software composition.With acetonitrile (A liquid) and volume fraction 0.01% phosphoric acid
Aqueous solution (B liquid) (pH=2.6) is flowing phase, uses binary gradient eluting (table 1).Detection wavelength is 270 nm, flow velocity 1.0m
L/min, column temperature 30 DEG C.Each sample repeats 3 times, and the TANSHINONES predominantly detected is cryptotanshinone, Tanshinone I and TANSHINONES
IIA, the TANSHINONES standard substance sample that detection uses is buied from Nat'l Pharmaceutical & Biological Products Control Institute (Beijing).
(4) result of the test
Salvia miltiorrhiza Growth situation and liposoluble constituent content to using the process of different disposal liquid to obtain detect, result such as following table
Shown in 2.As can be seen from the table, plant growing inorganic agent of the present invention can promote that Salvia miltiorrhiza f. grows, and also can improve it fat-soluble
Growth and the liposoluble constituent content of Salvia miltiorrhiza f. are all had by the change of component content, plant growing inorganic agent composition and content
Impact.Wherein, the inorganic agent best results of embodiment 9.Additionally, it is permissible by the experimental result contrast of embodiment 1 and distilled water
Finding out, alone wood vinegar is inconspicuous to Salvia miltiorrhiza f. growth promotion and liposoluble constituent accumulation effect.
Claims (10)
1. a medicinal plants growth process agent, is characterized in that: effective ingredient is wood vinegar, boric acid, LaCl3, vitamin B, carboxylic first
Base enclosure polysaccharide, Radix Salviae Miltiorrhizae fibrous root lixiviating solution and Chinese medicine extraction liquid, described wood vinegar is by Magnolia denudata, Chinese scholar tree and yellow rose dry distilling gained
Dry distillation liquid and water are obtained by mixing by the volume ratio of 1:500-2000.
Medicinal plants growth process agent the most according to claim 1, is characterized in that: described Chinese medicine extraction liquid is Rhizoma Atractylodis and purple
The water decoction of flower Herba Violae, or the water decoction of Rhizoma Atractylodis, Herba Violae, Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii；Described Radix Salviae Miltiorrhizae palpus
Root lixiviating solution is the water extract of Radix Salviae Miltiorrhizae fibrous root.
Medicinal plants growth process agent the most according to claim 2, is characterized in that: the mass ratio of Rhizoma Atractylodis and Herba Violae is
1:2-5；The mass ratio of Rhizoma Atractylodis, Herba Violae, Semen Arecae, Herba Scutellariae Barbatae and Fructus Cnidii is 1:2-5:5-10:0.1-0.3:5-10.
Medicinal plants growth process agent the most according to claim 3, is characterized in that: the mass ratio of Rhizoma Atractylodis and Herba Violae is
1:3；The mass ratio of Rhizoma Atractylodis, Herba Violae, Cortex Meliae, Herba Scutellariae Barbatae and Fructus Cnidii is 1:3:6:0.2:8.
5., according to the medicinal plants growth process agent according to any one of claim 1-4, it is characterized in that: Magnolia denudata, Chinese scholar tree and Huang
The mass ratio of thorn rose is 2:2:3.
6., according to the medicinal plants growth process agent according to any one of claim 1-4, it is characterized in that: boric acid is in wood vinegar
Mass concentration be 5-15%, LaCl3Content in wood vinegar is 2 × 10-4～10 × 10-4Mol/L, vitamin B is at wood vinegar
Content in liquid is 0.5-5%, and carboxymethyl chitosan content in wood vinegar is 1-6%, and Radix Salviae Miltiorrhizae fibrous root lixiviating solution is at wood vinegar
In mass concentration be 0～5%, Chinese medicine extraction liquid mass concentration in wood vinegar is 15-30%.
7., according to the medicinal plants growth process agent according to any one of claim 1-6, it is characterized in that: boric acid is in wood vinegar
Mass concentration be 10%, LaCl3Content in wood vinegar is 6.5 × 10-4Mol/L, vitamin B content in wood vinegar
Being 2%, carboxymethyl chitosan content in wood vinegar is 3%, and Radix Salviae Miltiorrhizae fibrous root lixiviating solution mass concentration in wood vinegar is
3%, Chinese medicine extraction liquid mass concentration in wood vinegar is 25%.
8., according to the medicinal plants growth process agent according to any one of claim 1-6, it is characterized in that: the preparation side of wood vinegar
Method is: Magnolia denudata, Chinese scholar tree and yellow rose are mixed, respectively 130～135 DEG C, 220～235 DEG C, do at 320～350 DEG C
Evaporate, merge the dry distillation liquid of gained at a temperature of these three sections, the dry distillation liquid remove impurity after merging, the body of 1:500-2000 is then pressed with water
The mixing of long-pending ratio, obtains wood vinegar.
9. a preparation method for the medicinal plants growth process agent according to any one of claim 1-8, is characterized in that including
Following steps:
(1) by Magnolia denudata, Chinese scholar tree and yellow rose respectively 130～135 DEG C, 220～235 DEG C, carry out dry distilling at 320～350 DEG C, close
And the dry distillation liquid of gained at a temperature of these three sections, the dry distillation liquid remove impurity after merging, the volume ratio of 1:500-2000 is then pressed with water
Mixing, obtains wood vinegar；
(2) traditional Chinese medicine powder is broken into fine powder, adds the soak by water 2-2.5h of they gross masses 10 times, filter, cool down, obtain Chinese medicine and carry
Take liquid；
(3) will pulverize after Radix Salviae Miltiorrhizae fibrous root drying at room temperature, cross 80 mesh sieves, add 1L distillation according to every 0.5g Radix Salviae Miltiorrhizae fibrous root ground product
The proportioning of water adds distilled water, extracts 24h, filter after extraction under room temperature, and gained filtrate is Radix Salviae Miltiorrhizae fibrous root lixiviating solution；
(4) in wood vinegar, boric acid, LaCl are added3, vitamin B, carboxymethyl chitosan, Radix Salviae Miltiorrhizae fibrous root lixiviating solution and Chinese medicine extraction
Liquid, mix homogeneously, obtain medicinal plants growth process agent.
10. the method promoting Salvia miltiorrhiza f. liposoluble constituent to accumulate, is characterized in that: in Salvia miltiorrhiza f. incubation, leaf
The medicinal plants growth process agent according to any one of claim 1-8 is sprayed in face, sprays weekly once, is drenched with blade face
Now drip and be advisable.
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