CN106035239B - A method of research walks quickly and keeps away substance to Oviposition of Diamondback Moth behavioral implications - Google Patents
A method of research walks quickly and keeps away substance to Oviposition of Diamondback Moth behavioral implications Download PDFInfo
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- CN106035239B CN106035239B CN201610371816.4A CN201610371816A CN106035239B CN 106035239 B CN106035239 B CN 106035239B CN 201610371816 A CN201610371816 A CN 201610371816A CN 106035239 B CN106035239 B CN 106035239B
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The present invention relates to insect lipids researchs, walk quickly and keep away substance by being added in liquid agar medium needed for experiment, have been prepared and can be used for studying the different oviposition bait bases for walking quickly and keeping away substance to Oviposition of Diamondback Moth behavioral implications.An enclosed environment is created to diamondback moth adult, and is put into oviposition bait base for its oviposition.Replace oviposition bait base, and statistical data in time according to demand.The oviposition phobotaxis for walking quickly and keeping away substance that diamondback moth walks quickly and keeps away difference substance or various concentration, preoviposition period duration, the oviposition behaviors data such as egg-laying time rule can be obtained according to data.The oviposition bait base has preferable hot melt, and can provide certain humidity, can have smooth surface under room temperature state, diamondback moth adult can be given to provide space support.The method of the present invention can be used for carrying out the parameters statistics of Oviposition of Diamondback Moth behavior under different situations, and environment probes into diamondback moth to different material oviposition phobotaxis indoors, and novel research method is provided for field insect control scheme.
Description
Technical field
The present invention relates to insect lipids research fields, specifically, being related to Oviposition of Diamondback Moth behavioral study.
Background technology
Diamondback moth is a kind of common crop in cruciferae pest, betides Mediterranean Region earliest, widely distributed
In all over the world, because its occurring area is huge, period of causing harm is long, and resistance development is rapid, and difficulty of prevention and cure is larger, instead of dark greyish green
Worm becomes brassicaceous vegetable number one killer.The fertility of diamondback moth is extremely strong, and under natural conditions, 150-400 are produced per female
Ovum can be such that population expands rapidly.It also has host extremely strong selectivity, goes out phobotaxis for different plant performances.It is right
Show special preferences in isothiocyanate content high crop, such as leaf mustard, Chinese cabbage etc., and on different hosts, lays eggs
Feature is also not quite similar, and, to dissipate production, some make heap for some.The oviposition behavior of diamondback moth and Populations of Diamondback Moth, Plutella Xylostella development are closely related,
The oviposition behavior of research diamondback moth can provide new approaches for field control；Finding Oviposition of Diamondback Moth phobotaxis substance can be anti-for field
Offer new method is provided.
Method currently used for Oviposition of Diamondback Moth behavior investigation includes crop field sampling statistics and laboratory plants, in vitro
Tissue raising statistics.Crop field sampling statistics are most common methods, which needs to complete in field and by weather and ring
Border is affected；Previous indoor statistical method needs to use plant or tissue, can not probe into diamondback moth for a certain specific object
The phobotaxis of matter oviposition, can not also study influence of the different material concentration (planting density) for Oviposition of Diamondback Moth behavior.
All fine jades etc. by the way that sample to be tested is added drop-wise on filter paper, have studied strumasterol substance to the oviposition of diamondback moth and
Influence (influence of the strumasterol substance to oviposition and the approach behavior selection of diamondback moth, all fine jades etc., the ring of approach behavior selection
Border insect journal, March 2011,33 (1):52-57).However the rough surface of filter paper and humidity are difficult to maintain in this method,
Diamondback moth be cannot be satisfied to the high humility of egg-laying environment and the requirement of smooth surface, cause diamondback moth to filter paper and ambient enviroment
Select similar temperament.The present invention provides a kind of oviposition bait base that can be maintained humidity for a long time and provide smooth surface, diamondback moth at
Worm is high to the selectivity of this bait base, and statistical data is accurate and replacement frequency is greatly reduced, and reduces cost of labor.
Invention content
In order to solve the problems in the existing technology, the object of the present invention is to provide a kind of researchs to walk quickly and keep away substance to pickles
The method of moth egg behavioral implications.
In order to realize the object of the invention, technical scheme is as follows：
A method of research walks quickly and keeps away substance to Oviposition of Diamondback Moth behavioral implications, using containing the oviposition bait for walking quickly and keeping away substance
Influence of the substance to Oviposition of Diamondback Moth behavior is walked quickly and kept away in base, research；It is described oviposition bait base ingredient include：Agar, walk quickly and keep away substance and
Antibiotic.
Optionally, the antibiotic is kanamycins.Addition kanamycins is used to prevent bacterium from growing on culture medium, because
Also it is round, the interference statistics similar with oval state of yellow for part bacterium colony.Other antibiosis are also replaced by according to actual conditions
Element.
Preferably, in the oviposition bait base, the mass concentration of agar is 1~2%.Concentration is too low, and agar block is not easily molded,
It is difficult to provide to adult and supports space；Concentration is too high, and rate of set is too fast, can not additive stir evenly.
Walking quickly and keeping away the concentration of substance can arbitrarily adjust according to experiment demand.This matrix can compare various concentration and walk quickly and keep away substance
Effect, therefore the concentration for walking quickly and keeping away substance is a variable.
The concentration of kanamycins is added according to the convention of general culture medium, preferably kanamycins final concentration 50ug/mL.
Further, the preparation method of the oviposition bait base is：It is added into the liquid agar medium not higher than 40 DEG C
It walks quickly and keeps away substance and kanamycins, stirs evenly, after cooled and solidified to obtain the final product.It will influence to walk quickly and keep away substance higher than 40 DEG C and kanamycins sent out
Wave effect.
Further, after stirring evenly, in packing to the culture dish of diameter 3cm, per ware 5mL.The dosage can guarantee production
The performance of corresponding agent effectiveness in ovum bait base, it is also ensured that certain level, and the thickness for bait base of laying eggs is moderate, is suitable for use in small
The research of diamond-back moth oviposition behavior.
Further, research is walked quickly and kept away substance and is specifically comprised the following steps to the method for Oviposition of Diamondback Moth behavioral implications：
(1) egg-laying environment makes：Arbitrarily sensitive pickles chrysalis similar in development condition will be put into insect cage, when in cage
When female adult starts to sprout wings, the oviposition bait base is placed in cage for its oviposition；
For the placement quantity of the oviposition bait base depending on pickles chrysalis quantity, every 3~4 pairs of pickles chrysalis configure an oviposition
Bait base；
(2) the frequency h of oviposition bait base is replaced according to experiment demand setting：When statistics oviposition characteristic, frequency h is set as 4
~12 hours；When statistics oviposition phobotaxis, frequency h is set as 12~48 hours；
(3) data collection：There is the batch N of oviposition bait base when first ovum grain；Simple grain ovum quantity a1With make heap pieces of an egg number
Measure a2；The ovum amount A of oviposition bait base and culture dish inner wall at n-th crowdn；Wherein, n is the batch of oviposition bait base；
In post-egg stage, when every ware ovum amount is less than 5, stop counting, and judge that oviposition terminates；
(4) preoviposition period calculates：H0=N (h-0.5)；
(5) egg laying amount counts：a1/a2>=1, judgement Oviposition of Diamondback Moth is to dissipate production, otherwise to make heap oviposition；
(6) egg-laying time rule counts：The eggs on average amount at the moment between (n-1) h to nh, diamondback moth is An/ h,
The egg laying amount that can regard as (n-0.5) the h moment, draws a diagram accordingly, analyzes Oviposition of Diamondback Moth temporal regularity；
(7) oviposition phobotaxis statistics：Count the oviposition total amount A=∑s A in diamondback moth entire egg-laying seasonn, examined not with t tests
With the significance of difference of experimental group.
The placement location of the oviposition bait base can be random, but relative to being centrally placed, and preferably dispersion is placed.
Preferably, in order to preferably make diamondback moth keep normal physiological condition, nutrient solution is placed in cage for diamondback moth
Adult sustains life.Optionally, the nutrient solution is hydromel.
Further, the step (1) sensitive pickles chrysalis similar in multipair development condition can be divided into it is multigroup into
Row observation, can also be adjusted to every group of single pair, and different repetitions is arranged according to requirement of experiment.
When numeration, if oviposition quantity is excessive and relatively uniform, cross hairs, sampling system can be made after the culture dish of oviposition bait base
Count the egg laying amount of 1/4 ware.
The beneficial effects of the present invention are：
The present invention walks quickly and keeps away substance by being added in liquid agar medium needed for experiment, has been prepared and can be used for grinding
Study carefully the different oviposition bait bases for walking quickly and keeping away substance to Oviposition of Diamondback Moth behavioral implications.An enclosed environment is created to diamondback moth adult, and
Oviposition bait base is put into for its oviposition.Replace oviposition bait base, and statistical data in time according to demand.Diamondback moth can be obtained according to data
The oviposition phobotaxis for walking quickly and keeping away substance of substance or various concentration, preoviposition period duration, the productions such as egg-laying time rule are walked quickly and kept away to difference
Ovum behavioral data.The oviposition bait base has preferable hot melt, and can provide certain humidity, can have under room temperature state smooth
Surface, can give diamondback moth adult provide space support.
Present invention materials are simple, and visual result is illustrated, and can be used for carrying out the items of Oviposition of Diamondback Moth behavior under different situations
Parametric statistics, indoors environment probe into diamondback moth to different material lay eggs phobotaxis, provided for field insect control scheme novel
Research method.It not only overcomes the territory restriction of conventional view method and diamondback moth can not be probed into become for a certain predetermined substance
The defect of keeping away property also overcomes and carries out diamondback moth to walking quickly and keeping away pickles in substance oviposition taxis statistic law using filter paper in the prior art
The moth defect not strong to filter paper selectivity.
Description of the drawings
Fig. 1 is oviposition substrate of the present invention and oviposition bait base.
Fig. 2 is the Oviposition of Diamondback Moth time statistics in the embodiment of the present invention 2.
Fig. 3 is the Oviposition of Diamondback Moth phobotaxis statistics in the embodiment of the present invention 3.
Specific implementation mode
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It will be appreciated that following real
Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this field
Art personnel can carry out various modifications and replace to the present invention without departing substantially from spirit of the invention and spirit.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
1 Oviposition of Diamondback Moth of embodiment measures early period
1. research object
The research object of the present embodiment is the Populations of Diamondback Moth, Plutella Xylostella in laboratory rearing mostly generation.
2. method
Oviposition of Diamondback Moth is lured using oviposition bait base of the present invention, counts preoviposition period length.
The preparation of 2.1 oviposition bait bases
1g agar powders are added in 45ml distilled water, 120 DEG C, 30 minutes high pressure steam sterilizations are cooled to 45 DEG C for use.?
In aforesaid liquid agar medium, 5ml distilled water is added, kanamycins 50ul (50mg/ml) stirs evenly rapidly, uses rubber head
Dropper is dispensed to diameter 3cm and is supported in ware, and per ware 5ml, room temperature cooled and solidified is obtained containing the oviposition bait base for walking quickly and keeping away substance.
2.2 egg-laying environments make：
Sensitive pickles chrysalis similar in 150 pairs of development conditions is divided into three groups, is put into insect cage.When female adult in cage
When starting to sprout wings, oviposition bait base is put into cage, placement position is random and disperses as possible, one changes for every eight hours.Bee is put into cage
Mulse sustains life for adult, maintains 20~25 DEG C of temperature, relative humidity 50%~75%, illumination 16L：8D.
2.3 data statistics
There are the following data of the oviposition bait base of Eggs of Diamondback Moth grain in record first：
N=3.
Occurs ovum grain for the first time on third oviposition bait base, the preoviposition period of diamondback moth is about 20 hours.
2 Oviposition of Diamondback Moth temporal regularity of embodiment
1. research object
The research object of the present embodiment is the Populations of Diamondback Moth, Plutella Xylostella in laboratory rearing mostly generation.
2. method
Oviposition of Diamondback Moth is lured using oviposition bait base of the present invention, counts preoviposition period length.
2.1 walk quickly and keep away substance making
Cabbage processing：Cabbage leaf, which is cleaned, to be dried, and is chosen vein fewer parts, is removed visible vein, for use.
Cabbage mesophyll 15g is weighed, is shredded.It puts to mortar, grinding is until tissue is destroyed completely repeatedly.It is leaked in taper
The upper 8 layers of gauze of pad in bucket, and soak.It is transferred in funnel with metal spoon by content is ground, squeezes filtering, a small amount of distilled water is used in combination
Mortar, spoon and grinding content are washed, squeezes filtering again.Filtrate is collected, 10ml is settled to distilled water.
The preparation of 2.2 oviposition bait bases
1g agar powders are added in 45ml distilled water, 120 DEG C, 30 minutes high pressure steam sterilizations are cooled to 45 DEG C for use.?
In aforesaid liquid agar medium, the above-mentioned inducing substances of 5ml are added, kanamycins 50ul (50mg/ml) is stirred evenly rapidly,
It is dispensed to diameter 3cm and is supported in ware with rubber head dropper, per ware 5ml, room temperature cooled and solidified obtains containing the oviposition bait base for walking quickly and keeping away substance,
The distilled water of substance equivalent is added and walked quickly and kept away in control group.
2.2 egg-laying environments make：
Sensitive pickles chrysalis similar in 150 pairs of development conditions is divided into three groups, is put into insect cage.When female adult in cage
When starting to sprout wings, oviposition bait base is put into cage, placement position is random and disperses as possible, changes within every 24 hours one.It is put into cage
Hydromel sustains life for adult, maintains 20~25 DEG C of temperature, relative humidity 50%~75%, illumination 16L：8D.
2.3 data statistics
Record Eggs of Diamondback Moth grain number amount in each ware.
Oviposition of Diamondback Moth time graph is rendered obvious by a peak (such as Fig. 2), and the peak that different inducing substances are presented is also different.
3 Oviposition of Diamondback Moth phobotaxis of embodiment counts
1. research object
The research object of the present embodiment is the Populations of Diamondback Moth, Plutella Xylostella in laboratory rearing mostly generation.
2. method
Oviposition of Diamondback Moth is lured using oviposition bait base of the present invention, counts preoviposition period length.
2.1 inducing substances make
Cabbage processing：Cabbage leaf, which is cleaned, to be dried, and is chosen vein fewer parts, is removed visible vein, for use.
Turnip Sprouts processing：The length of health is chosen to the Turnip Sprouts of 6cm, cuts plumular axis, collection belt cotyledon part, and remove cotyledon
Kind of a shell seedling is not gone out, for use.
Cabbage mesophyll or Turnip Sprouts 15g are weighed, is shredded.It puts to mortar, grinding is until tissue is broken completely repeatedly
It is bad.Upper 8 layers of gauze is padded in conical hopper, and is soaked.It is transferred in funnel with metal spoon by content is ground, squeezes filtering, and
With a small amount of distillation water washing mortar, spoon and grinding content squeeze filtering again.Filtrate is collected, is settled to distilled water
10ml。
2.2 oviposition substrates are prepared
1g agar powders are added in 45ml distilled water, 120 DEG C, 30 minutes high pressure steam sterilizations are cooled to 45 DEG C for use.?
In aforesaid liquid agar medium, the above-mentioned inducing substances of 5ml are added, kanamycins 50ul (50mg/ml) is stirred evenly rapidly,
It is dispensed to diameter 3cm and is supported in ware with rubber head dropper, per ware 5ml, room temperature cooled and solidified obtains containing the oviposition bait base for walking quickly and keeping away substance,
It is added and walks quickly and keeps away the distilled water of substance equivalent in control group (see Fig. 1).
2.2 egg-laying environments make：
Sensitive pickles chrysalis similar in 150 pairs of development conditions is divided into three groups, is put into insect cage.When female adult in cage
When starting to sprout wings, oviposition bait base is put into cage, placement position is random and disperses as possible, one changes for every eight hours.Bee is put into cage
Mulse sustains life for adult, maintains 20~25 DEG C of temperature, relative humidity 50%~75%, illumination 16L：8D.
2.3 data statistics
Record Eggs of Diamondback Moth grain number amount in each ware.
Ovum grain accumulation result such as Fig. 3, is test by t on the oviposition bait base of different disposal, can intuitively obtain diamondback moth for
The phobotaxis of different material.
Although above the present invention is described in detail with a general description of the specific embodiments, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention belong to the scope of protection of present invention.
Claims (9)
1. method of the substance to Oviposition of Diamondback Moth behavioral implications is walked quickly and kept away in a kind of research, which is characterized in that using containing walking quickly and keeping away substance
Oviposition bait base, research walk quickly and keep away influence of the substance to Oviposition of Diamondback Moth behavior；It is described oviposition bait base ingredient include：Agar becomes
Keep away substance and antibiotic；
Described method includes following steps：
(1) egg-laying environment makes：Arbitrarily sensitive pickles chrysalis similar in development condition will be put into insect cage, when female adult in cage
When starting to sprout wings, the oviposition bait base is placed in cage for its oviposition；
For the placement quantity of the oviposition bait base depending on pickles chrysalis quantity, every 3~4 pairs of pickles chrysalis configure an oviposition bait
Base；
(2) the frequency h of oviposition bait base is replaced according to experiment demand setting：When statistics oviposition characteristic, frequency h is set as 4~12
Hour；When statistics oviposition phobotaxis, frequency h is set as 12~48 hours；
(3) data collection：There is the batch N of oviposition bait base when first ovum grain；Simple grain ovum quantity a1With make heap pieces of an egg quantity a2；
The ovum amount A of oviposition bait base and culture dish inner wall at n-th crowdn；Wherein, n is the batch of oviposition bait base；
In post-egg stage, when every ware ovum amount is less than 5, stop counting, and judge that oviposition terminates；
(4) preoviposition period calculates：H0=N (h-0.5)；
(5) egg laying amount counts：a1/a2>=1, judgement Oviposition of Diamondback Moth is to dissipate production, otherwise to make heap oviposition；
(6) egg-laying time rule counts：The eggs on average amount at the moment between (n-1) h to nh, diamondback moth is An/ h can also assert
It for the egg laying amount at (n-0.5) h moment, draws a diagram accordingly, analyzes Oviposition of Diamondback Moth temporal regularity；
(7) oviposition phobotaxis statistics：Count the oviposition total amount A=∑s A in diamondback moth entire egg-laying seasonn, different realities are examined with t tests
Test the significance of difference of group.
2. according to the method described in claim 1, it is characterized in that, in the oviposition bait base, the mass concentration of agar is 1~
2%.
3. method according to claim 1 or 2, which is characterized in that it is described oviposition bait base preparation method be：To not higher than
It is added in 40 DEG C of liquid agar medium and walks quickly and keeps away substance and kanamycins, stirred evenly, after cooled and solidified to obtain the final product.
4. according to the method described in claim 3, it is characterized in that, after stirring evenly, dispense into the culture dish of diameter 3cm,
Per ware 5mL.
5. according to the method described in claim 1, it is characterized in that, oviposition bait base dispersion is placed.
6. according to the method described in claim 5, maintaining to give birth to for diamondback moth adult it is characterized in that, placing nutrient solution in cage
Life.
7. according to the method described in claim 6, it is characterized in that, the nutrient solution is hydromel.
8. according to the method described in claim 1, it is characterized in that, the step (1) can will be quick similar in multipair development condition
Sense pickles chrysalis be divided into it is multigroup observed, can also be adjusted to every group of single pair, and different weights is set according to requirement of experiment
It is multiple.
If 9. according to the method described in claim 1, it is characterized in that, oviposition quantity it is excessive and relatively uniform, can oviposition bait
Make cross hairs, the egg laying amount of 1/4 ware of sampling statistics after the culture dish of base.
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| CN107801697A (en) * | 2017-11-02 | 2018-03-16 | 山东省农业科学院植物保护研究所 | A kind of apparatus and method for monitoring and studying insect taxis behavior |
| CN109662063A (en) * | 2018-12-18 | 2019-04-23 | 河南农业大学 | A kind of quick screening lepidopterous insects oviposition is lured or the oviposition cage and its application method of repellent activity substance |
| CN115462374B (en) * | 2022-09-05 | 2024-01-26 | 山西农业大学 | Volatile substance in herba Menthae with repellent activity for Plutella xylostella |
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| CN101223890A (en) * | 2008-01-31 | 2008-07-23 | 华南农业大学 | Egg-laying preventer and preparing method thereof for diamondback moth |
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| JP6467177B2 (en) * | 2013-09-30 | 2019-02-06 | 国立研究開発法人農業・食品産業技術総合研究機構 | Natural enemy insect attractant component derived from floral scent and its use |
| US20160081352A1 (en) * | 2014-09-23 | 2016-03-24 | Luis Augusto Mazariegos | Pest control formulation of Neem and Beauveria bassiana and methods of making and using same |
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|---|---|---|---|---|
| CN101223890A (en) * | 2008-01-31 | 2008-07-23 | 华南农业大学 | Egg-laying preventer and preparing method thereof for diamondback moth |
| CN102106318A (en) * | 2011-01-17 | 2011-06-29 | 广东出入境检验检疫局检验检疫技术中心 | Culture device for white fly and tetranychid mite feeding experiment and method for conducting experiment using same |
| CN103766407A (en) * | 2013-12-24 | 2014-05-07 | 华南农业大学 | Isaria fumosorosea and abamectin oil suspension agent as well as preparation method and application thereof |
| CN104126614A (en) * | 2014-08-18 | 2014-11-05 | 熊吉元 | Preparation method for biological pesticide |
| CN104381334A (en) * | 2014-12-20 | 2015-03-04 | 唐睿 | Formula and preparation method of micro-capsule suspoemulsion containing abamectin and thymol |
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