CN101223890A - Egg-laying preventer and preparing method thereof for diamondback moth - Google Patents
Egg-laying preventer and preparing method thereof for diamondback moth Download PDFInfo
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- CN101223890A CN101223890A CNA2008100261985A CN200810026198A CN101223890A CN 101223890 A CN101223890 A CN 101223890A CN A2008100261985 A CNA2008100261985 A CN A2008100261985A CN 200810026198 A CN200810026198 A CN 200810026198A CN 101223890 A CN101223890 A CN 101223890A
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- lantana
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- back moth
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Abstract
The invention discloses a diamondback moth oviposition inhibitor, characterized in that the inhibitor comprises a common lantana extractive solution, water and a surfactant. The volume ratio of the common lantana extractive solution, the water and the surfactant is 3:100-400:0.3-2. The invention can be directly sprayed on the surface of a cruciferous vegetable plant and can reduce the ovum attaching amount of the adult diamondback moth on the cruciferous vegetable plant as well as have significant spawning inhibition effect to the diamondback moth so as to reduce the population quantity of the moth on the cruciferous vegetable plant, thus having the function of protecting the vegetable. Meanwhile, the invention takes full advantage of the common lantana, a 'harmful herb' and provides a feasible way for 'eliminating harm with harmful herb and changing the harmful herb into useful substance' for the common lantana.
Description
Technical field
The present invention relates to the plant resource plant protection product, be specifically related to a kind of diamond-back moth oviposition inhibitor and preparation method thereof.
Background technology
Diamond-back moth (Plutella xylostella) is one of primary pest on China southern area vegetables produce.Along with a large amount of uses of chemical pesticide, the pesticide resistance of this worm rises significantly, and it is also more and more serious to cause harm.The sustainable Control principle of this and diamond-back moth is not inconsistent.Utilize the correlation of plant secondary substance and insect, set up the control of insect system, can realize sustainable Control (Pang Xiongfei, world technology research and development, Vol.21, NO.10.pp24-28,1999 of insect; Pickett J A etc., Agriculture Ecosystems and Environment64:149-156,1997).Laying eggs is the important step of insect population development, reduce insect on target plant the ovum amount, can effectively reduce the population quantity of insect on this plant, thereby realize control (Pang Xiongfei etc., Chinese Journal of Applied Ecology, Vol.11 to insect, No.1.pp108～110,2000).
Lantana (Lantana camara L.) has another name called Lantana camara, the grass of complying with one's wishes, and is Verenaceae (Verbenaceae) Lantana (Lantana) plant.This plant originates in the torrid zone and the subtropical zone in America, and its breeding, adaptive capacity are strong, occupy large-area pasture and arable land, are regarded as one of 10 kinds of weeds the most harmful in the world.Its pattern is various and bright-coloured, is usually used in gardens and decorates, and all has wild and cultivates in province such as Guangdong, Guangxi and Fujian of China.Lantana has good prospects for application (Kong CH etc., Weed Research 46:290-295) aspect control of insect, its extract reduce diamond-back moth on cabbage heart the ovum amount, suppress the population growth (Pang Xiongfei etc. of this worm on cabbage heart, Chinese Journal of Applied Ecology, Vol.11, No.1.pp108～110,2000), the total lantadene of lantana to diamondback moth larvae have significant food refusal effect (Dong Yi etc., Chinese Journal of Applied Ecology, Vol.16, No.12.Pp2361-2364,2005).More than research is inquired into the biologically active of diamond-back moth with regard to the lantana extract from theory point of view.
But domestic do not see as yet the lantana extract is made the report that preparation is applied to diamond-back moth control.Therefore, develop a kind of preparation that can be applicable to the lantana extract of production practices, can be lantana in the utilization of diamond-back moth aspect preventing and treating, for lantana " control evil with evil, become evil into " a practicable approach is provided.
Summary of the invention
The objective of the invention is day benefit the deficiencies in the prior art, a kind of diamond-back moth oviposition inhibitor is provided.
Another object of the present invention provides the preparation method of described diamond-back moth oviposition inhibitor.
Purpose of the present invention is achieved by the following technical programs:
A kind of diamond-back moth oviposition inhibitor is provided, is made up of lantana extract solution, water and surfactant, the volume ratio of described lantana extract solution, water and surfactant is 3: 100～400: 0.3～2.
The preparation method of described lantana extract solution is: with the methanolic extract of 1 weight portion lantana or chloroform extract with the ethanol of 10～15 weight portions in water-bath fully dissolving prepare; Or with total lantadene of 1 weight portion with 10～15 weight portion volume ratios be ethanol-acetone mixed liquor of 2: 1 in water-bath fully dissolving prepare.
The described bath temperature of above-mentioned preparation method is 60～70 ℃.
The methanolic extract of described lantana is that the lantana blade is dried to constant weight at 50～70 ℃, is ground into dry powder, soaks dry powder with methyl alcohol at normal temperatures, filters, and gets methanol extract liquid; It is plain to golden yellow that methanol extract liquid is discolored with activated carbon, and filtration is removed methyl alcohol and prepared.
The chloroform extract of described lantana is to adopt the methanolic extract of lantana to soak with chloroform, filters, and removes chloroform and prepares.
Described total lantadene is to adopt an amount of methyl alcohol 60～70 ℃ chloroform extract to be dissolved fully in water-bath, in leaving standstill below 0 ℃ 24 hours, treats that after crystal is separated out crystal being filtered nature dries the yellow powder that obtains.
Described surfactant is a polysorbas20.
The present invention provides the preparation method of described diamond-back moth oviposition inhibitor simultaneously, with mixing in water and the surfactant adding lantana extract solution promptly.
The invention has the beneficial effects as follows: the diamond-back moth oviposition inhibitor can reduce the diamond-back moth adult on the brassicaceous vegetable plant the ovum amount, this worm there is the significant oviposition inhibitory action, this inhibitor can directly be sprayed on brassicaceous vegetable plant surface, reduce the diamond-back moth adult on plant corpus the ovum amount, reduce the population quantity of this worm on brassicaceous vegetable, thereby such vegetables are played a protective role; The present invention has made full use of " evil grass " lantana, for lantana " control evil with evil, become evil into " a practicable approach is provided.
Embodiment
Further specify the present invention below in conjunction with specific embodiment.
Embodiment 1
The lantana blade is dried to constant weight at 50～70 ℃, be ground into dry powder, soak lantana dry powder according to conventional method and consumption with methyl alcohol at normal temperatures, filter, get methanol extract liquid; It is plain to golden yellow that methanol extract liquid is discolored with activated carbon, and the methanolic extract that methyl alcohol prepares lantana is removed in filtration; The methanolic extract of lantana soaks with chloroform, filters, and removes the chloroform extract that chloroform prepares lantana; Adopt an amount of methyl alcohol 60～70 ℃ chloroform extract to be dissolved fully,, treat that after crystal is separated out crystal being filtered nature dries the total lantadene of yellow powder that obtains in leaving standstill below 0 ℃ 24 hours in water-bath.The method that adopts is all this area conventional method.
Embodiment 2
Get 0.2g lantana methanolic extract, with 2ml ethanol it dissolved, in methanolic extract solution, add 400ml water and 2ml polysorbas20 in 60～70 ℃ of water-baths, mixing, preparation 1.
Get the chloroform extract of 0.2g lantana methanolic extract, with 2.5ml ethanol it dissolved, in chloroform extract solution, add 300ml water and 0.5ml polysorbas20 in 60～70 ℃ of water-baths, mixing, preparation 2.
Getting the total lantadene of component of 0.2g chloroform extract, is that ethanol-acetone mixed liquor of 2: 1 dissolves it in 60～70 ℃ of water-baths with the 2ml volume ratio, adds 400ml water and 2ml polysorbas20 in total lantadene solution, mixing, preparation 3.
Embodiment 3
Get 0.2g lantana methanolic extract, with 3ml ethanol it dissolved, in methanolic extract solution, add 100ml water and 0.3ml polysorbas20 in 70 ℃ of water-baths, mixing, preparation 4.Get the chloroform extract of 0.2g lantana methanolic extract, with 3ml ethanol it dissolved, in chloroform extract solution, add 100ml water and 0.3ml polysorbas20 in 70 ℃ of water-baths, mixing, preparation 5.Getting the total lantadene of component of 0.2g chloroform extract, is that ethanol-acetone mixed liquor of 2: 1 dissolves it in 70 ℃ of water-baths with the 3ml volume ratio, adds 100ml water and 0.3ml polysorbas20 in total lantadene solution, mixing, preparation 6.Measure 4,5 and 6 pairs of influences that diamond-back moth lays eggs of preparation with shrouding method:
Have the blue seedling of Jie of 5 true leaves to plant in plastic cup with long, diameter is 4cm at the bottom of the plastic cup, and the rim of a cup diameter is 6.5cm, and height is 8cm, respectively preparation 4,5 and 6 is sprayed on leave dual sides with manual sprayer.In 100ml water, add 3ml ethanol and 0.3ml polysorbas20 as contrast, with manual sprayer it is sprayed on the positive and negative of the blue blade that is situated between equally.After waiting to evaporate, the cabbage heart seedling that is sprayed with preparation and contrast is moved into dependent insect cage simultaneously, dependent insect cage 60cm * 60cm lines up 4 row, every row 5 strains by randomised block design.20 pairs of 3 days diamond-back moth adults that sprouted wings are inserted dependent insect cage.Investigate every day this worm on the blue plant that is situated between the ovum amount, investigate 5 days.Experimental result shows, preparation 4,5 and 6 reduce the diamond-back moth adults handle on the plant 69.2～78.2% the ovum amount, this one-tenth is had the significant oviposition inhibitory action.And, after using the 5th day, preparation 4,5 and 6 still can play the significant oviposition inhibitory action to the diamond-back moth adult, reduce respectively this worm handle on the plant 48.4%, 62.8% and 54.2% the ovum amount.
Embodiment 4
Get the chloroform extract of 0.2g lantana methanolic extract, with 3ml ethanol it dissolved, in chloroform extract solution, add 200ml water and 0.6ml polysorbas20 in 70 ℃ of water-baths, mixing, preparation 7.Get the chloroform extract of 0.2g lantana methanolic extract, with 3ml ethanol it dissolved, in chloroform extract solution, add 400ml water and 1.2ml polysorbas20 in 70 ℃ of water-baths, mixing, preparation 8.The method of shrouding by embodiment 3 is measured preparation 7 and 8 pairs of influences that the diamond-back moth adult lays eggs.
Experimental result shows, preparation 7 and 8 reduce respectively the diamond-back moth adult handle on the plant 51.5% and 54.4% the ovum amount, this worm is had the significant oviposition inhibitory action.And, after using the 6th day, preparation 7 and 8 still can play significant inhibitory effect to the diamond-back moth adult, reduce respectively this worm handle on the plant 55.7% and 56.4% the ovum amount.
Claims (8)
1. a diamond-back moth oviposition inhibitor is characterized in that being made up of lantana extract solution, water and surfactant, and the volume ratio of described lantana extract solution, water and surfactant is 3: 100～400: 0.3～2.
2. diamond-back moth oviposition inhibitor according to claim 1 is characterized in that the preparation method of described lantana extract solution is: with the methanolic extract of 1 weight portion lantana or chloroform extract with the ethanol of 10～15 weight portions in water-bath fully dissolving prepare; Or with total lantadene of 1 weight portion with 10～15 weight portion volume ratios be ethanol-acetone mixed liquor of 2: 1 in water-bath fully dissolving prepare.
3. diamond-back moth oviposition inhibitor according to claim 2 is characterized in that described bath temperature is 60～70 ℃.
4. diamond-back moth oviposition inhibitor according to claim 2, the methanolic extract that it is characterized in that described lantana are that the lantana blade is dried to constant weight at 50～70 ℃, are ground into dry powder, soak dry powder with methyl alcohol at normal temperatures, filter, and get methanol extract liquid; It is plain to golden yellow that methanol extract liquid is discolored with activated carbon, and filtration is removed methyl alcohol and prepared.
5. diamond-back moth oviposition inhibitor according to claim 2, the chloroform extract that it is characterized in that described lantana are to adopt the methanolic extract of lantana to soak with chloroform, filter, and remove chloroform and prepare.
6. diamond-back moth oviposition inhibitor according to claim 2, it is characterized in that described total lantadene is to adopt an amount of methyl alcohol 60～70 ℃ chloroform extract to be dissolved fully in water-bath, in leaving standstill below 0 ℃ 24 hours, treat that after crystal is separated out crystal being filtered nature dries the yellow powder that obtains.
7. diamond-back moth oviposition inhibitor according to claim 1 is characterized in that described surfactant is a polysorbas20.
8. the preparation method of the described diamond-back moth oviposition inhibitor of claim 1 is characterized in that with mixing in water and the surfactant adding lantana extract solution promptly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100261985A CN101223890B (en) | 2008-01-31 | 2008-01-31 | Egg-laying preventer and preparing method thereof for diamondback moth |
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| CN2008100261985A CN101223890B (en) | 2008-01-31 | 2008-01-31 | Egg-laying preventer and preparing method thereof for diamondback moth |
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| CN101223890A true CN101223890A (en) | 2008-07-23 |
| CN101223890B CN101223890B (en) | 2011-02-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655945A (en) * | 2013-12-26 | 2014-03-26 | 佛山市梦莎美容化妆品有限公司 | Key production technology and authentication method for bluegrass activated extract |
| CN106035239A (en) * | 2016-05-30 | 2016-10-26 | 中国农业大学 | Method for studying influences exerted by approach-avoidance substances on oviposition behaviors of diamondback moths |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1244279C (en) * | 2004-07-20 | 2006-03-08 | 中国科学院上海生命科学研究院 | Water hyacinth biological inhibitor and its preparing method |
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2008
- 2008-01-31 CN CN2008100261985A patent/CN101223890B/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103655945A (en) * | 2013-12-26 | 2014-03-26 | 佛山市梦莎美容化妆品有限公司 | Key production technology and authentication method for bluegrass activated extract |
| CN106035239A (en) * | 2016-05-30 | 2016-10-26 | 中国农业大学 | Method for studying influences exerted by approach-avoidance substances on oviposition behaviors of diamondback moths |
| CN106035239B (en) * | 2016-05-30 | 2018-11-09 | 中国农业大学 | A method of research walks quickly and keeps away substance to Oviposition of Diamondback Moth behavioral implications |
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| Publication number | Publication date |
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| CN101223890B (en) | 2011-02-16 |
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Effective date of registration: 20160411 Address after: 510530 Guangdong province high tech Industrial Development Zone of Guangzhou city science and technology innovation base on Road No. 80 D District 607-612 unit Patentee after: GUANGZHOU RUIFENG BIOTECHNOLOGY CO., LTD. Address before: 510642 Guangdong city of Guangzhou province Tianhe District Wushan Patentee before: South China Agricultural University |
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Inventor after: Lu Yongyue Inventor after: Zhang Maoxin Inventor after: Dong Yizhi Inventor after: Ling Bing Inventor after: Liang Guangwen Inventor after: Song Yanxia Inventor after: Li Shenlei Inventor after: Liu Huan Inventor before: Zhang Maoxin Inventor before: Dong Yizhi Inventor before: Ling Bing Inventor before: Liang Guangwen Inventor before: Song Yanxia |
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