CN101223889B - Food-intake preventer and preparing method thereof for striped flea beetle - Google Patents
Food-intake preventer and preparing method thereof for striped flea beetle Download PDFInfo
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- CN101223889B CN101223889B CN2008100261951A CN200810026195A CN101223889B CN 101223889 B CN101223889 B CN 101223889B CN 2008100261951 A CN2008100261951 A CN 2008100261951A CN 200810026195 A CN200810026195 A CN 200810026195A CN 101223889 B CN101223889 B CN 101223889B
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- lantana
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- phyllotreta striolata
- methyl alcohol
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Abstract
The invention discloses a phyllotreta striolata perfect insect food intake inhibitor which comprises a common lantana extractive solution, water and a surfactant. The volume ratio of the common lantana extractive solution, the water and the surfactant is 2:100-400:0.5-2. The invention can be directly sprayed on the surface of a cruciferous vegetable plant and can inhibit the food intake of the phyllotreta striolata perfect insect, resulting in the significant food refusal effect to the insect so as to reduce the harm on the cruciferous vegetable plant, thus protecting the vegetable plant. The invention pertains to a botanical plant protection agent and provides a feasible way for 'eliminating harm with harmful herb and changing the harmful herb into useful substance' for common lantana.
Description
Technical field
The present invention relates to the plant resource plant protection product, be specifically related to a kind of phyllotreta striolata imago feeding inhibitor and preparation method thereof.
Background technology
Phyllotreta striolata (Phyllotreta striolata) is the primary pest of brassicaceous vegetable, is distributed in more than 50 country.At China's southern area, be one of primary pest on vegetables produce.At present, the control to phyllotreta striolata is main with the chemical control.Along with the consumption increase of chemical pesticide, the pesticide resistance of phyllotreta striolata worm rises significantly, and insect pest is also more and more serious.Utilize the food refusal effect of plant secondary substance, set up the control of insect system, can realize sustainable Control (Pang Xiongfei, world technology research and development, Vol.21, NO.10.pp24-28,1999 of insect insect; Pickett J A etc., Agriculture Ecosystems andEnvironment 64:149-156,1997).
Lantana (Lantana camara L.) has another name called Lantana camara, the grass of complying with one's wishes, and is Verenaceae (Verbenaceae) Lantana (Lantana) plant.Lantana originates in the torrid zone and the subtropical zone in America, and breeding, adaptive capacity is strong, occupies large-area pasture and arable land, is regarded as one of 10 kinds of weeds the most harmful in the world.Its pattern is various and bright-coloured, is usually used in gardens and decorates, and all has wild and cultivates in province such as Guangdong, Guangxi and Fujian of China.Lantana has good prospects for application (Kong CH etc., Weed Research 46:290-295) aspect control of insect, its extract has significant repellent and food refusal effect (Zhao Hui etc. to phyllotreta striolata, Agricultural University Of South China's journal, Vol.24, No.3.pp38-40,2003; Yan Zhenmin etc., insect knowledge, Vol.42, No.6.pp664-668,2005).More than research is inquired into the biologically active of phyllotreta striolata with regard to the lantana extract from theory point of view.
But domestic do not see as yet the lantana extract is made the report that preparation is applied to phyllotreta striolata control.Therefore, develop a kind of preparation that can be applicable to the lantana extract of production practices, can be lantana in the utilization of phyllotreta striolata aspect preventing and treating, for lantana " control evil with evil, become evil into " a practicable approach is provided.
Summary of the invention
The objective of the invention is to remedy the deficiencies in the prior art, a kind of phyllotreta striolata imago feeding inhibitor is provided.
Another object of the present invention provides the preparation method of described phyllotreta striolata imago feeding inhibitor.
Purpose of the present invention is achieved by the following technical programs:
A kind of phyllotreta striolata imago feeding inhibitor is provided, is made up of lantana extract solution, water and surfactant, the volume ratio of described lantana extract solution, water and surfactant is 2: 100～400: 0.5～2.
The preparation method of described lantana extract solution is: with the methanolic extract of 1 weight portion lantana or chloroform extract with the methyl alcohol of 5～10 weight portions in water-bath fully dissolving prepare; Or with total lantadene of 1 weight portion with 9～15 weight portion volume ratios be methyl alcohol-chloroform mixed liquor of 3: 1 in water-bath fully dissolving prepare.
The described bath temperature of above-mentioned preparation method is 60～70 ℃.
The methanolic extract of described lantana is that the lantana blade is dried to constant weight at 50～70 ℃, is ground into dry powder, soaks dry powder with methyl alcohol at normal temperatures, filters, and gets methanol extract liquid; Methanol extract liquid discolors plain to golden yellow with activated carbon, filtration is removed methyl alcohol and prepared.
The chloroform extract of described lantana is to adopt the methanolic extract of lantana to soak with chloroform, filters, and removes chloroform and prepares.
Described total lantadene is to adopt an amount of methyl alcohol 60～70 ℃ chloroform extract to be dissolved fully in water-bath, in leaving standstill below 0 ℃ 24 hours, treats that after crystal is separated out crystal being filtered nature dries the yellow powder that obtains.
Described surfactant can be selected Tween 80 for use.
The present invention provides the preparation method of described phyllotreta striolata imago feeding inhibitor simultaneously, and water and surfactant are added the lantana extract solution, and mixing promptly.
The invention has the beneficial effects as follows:
(1) phyllotreta striolata imago feeding inhibitor of the present invention can suppress the phyllotreta striolata imago feeding, and this worm is had significant food refusal effect.This inhibitor can directly be sprayed on brassicaceous vegetable plant surface, and it can suppress phyllotreta striolata imago feeding plant corpus, reduces the cause harm degree of this worm to brassicaceous vegetable, thereby plant corpus is played a protective role.
(2) the invention provides a kind of lantana extract formulation, be applied to the phyllotreta striolata control, easily and effectively.
(3) the invention belongs to the plant resource plant protection product, for lantana " control evil with evil, become evil into " a practicable approach is provided.
Embodiment
Further specify the present invention below in conjunction with specific embodiment.
Embodiment 1
The lantana blade is dried to constant weight at 50～70 ℃, be ground into dry powder, soak lantana dry powder according to conventional method and consumption with methyl alcohol at normal temperatures, filter, get methanol extract liquid; It is plain to golden yellow that methanol extract liquid is discolored with activated carbon, and the methanolic extract that methyl alcohol prepares lantana is removed in filtration; The methanolic extract of lantana soaks with chloroform, filters, and removes the chloroform extract that chloroform prepares lantana; Adopt an amount of methyl alcohol 60～70 ℃ chloroform extract to be dissolved fully,, treat that after crystal is separated out crystal being filtered nature dries the total lantadene of yellow powder that obtains in leaving standstill below 0 ℃ 24 hours in water-bath.The method that adopts is all this area conventional method.
Embodiment 2
Get 0.2g lantana methanolic extract, with 2ml methyl alcohol it is dissolved, obtain the lantana extract solution in 60～70 ℃ of water-baths; Add 100ml water and 0.5ml Tween 80 in the lantana extract solution, mixing gets preparation 1.
Embodiment 3
Get 0.2g lantana methanolic extract, with 1ml methyl alcohol it is dissolved, obtain the lantana extract solution in 60～70 ℃ of water-baths; Add 400ml water and 1ml Tween 80 in the lantana extract solution, mixing gets preparation 2.
Embodiment 4
Get 0.2g lantana methanolic extract, with 1.5ml methyl alcohol it is dissolved, obtain the lantana extract solution in 60～70 ℃ of water-baths; Add 200ml water and 2ml Tween 80 in the lantana extract solution, mixing gets preparation 3.
Embodiment 5
Get the chloroform extract of 0.2g lantana methanolic extract, with 2ml methyl alcohol it dissolved, in chloroform extract solution, add 100ml water and 0.5ml Tween 80 in 60～70 ℃ of water-baths, mixing, preparation 4.
Embodiment 6
Getting the total lantadene of component of 0.2g chloroform extract, is that methyl alcohol-chloroform mixed liquor of 1.5: 0.5 dissolves it in 60～70 ℃ of water-baths with the 2ml volume ratio, adds 100ml water and 0.5ml Tween 80 in total lantadene solution, mixing, preparation 5.
Embodiment 7
Getting the total lantadene of component of 0.2g chloroform extract, is that methyl alcohol-chloroform mixed liquor of 3: 1 dissolves it in 60～70 ℃ of water-baths with the 1.8ml volume ratio, adds 200ml water and 1ml Tween 80 in total lantadene solution, mixing, preparation 6.
Embodiment 8
Getting the total lantadene of component of 0.2g chloroform extract, is that methyl alcohol-chloroform mixed liquor of 3: 1 dissolves it in 60～70 ℃ of water-baths with the 3ml volume ratio, adds 400ml water and 2ml Tween 80 in total lantadene solution, mixing, preparation 7.
Embodiment 9
Measure of the influence of the foregoing description 2～8 preparations with the method for shrouding to the phyllotreta striolata imago feeding:
Have the cabbage heart seedling of 5 true leaves to plant in plastic cup with long, diameter is 4cm at the bottom of the plastic cup, and the rim of a cup diameter is 6.5cm, and height is 8cm, respectively preparation is sprayed on leave dual sides with manual sprayer.In 100ml water, add 2ml methyl alcohol and 0.5ml Tween 80 as contrast, with manual sprayer it is sprayed on the positive and negative of cabbage heart blade equally.
After waiting to evaporate, the cabbage heart seedling that is sprayed with preparation and contrast is moved into dependent insect cage simultaneously, dependent insect cage 40cm * 40cm lines up 4 row, every row 3 strains by randomised block design.The phyllotreta striolata adult of 120 hungry 5h is inserted dependent insect cage.That investigates adult every day gets the food area, investigates 5 days.Experimental result shows that embodiment 2～8 preparations all significantly suppress the food of getting of phyllotreta striolata adult, reduce this worm handle on the blade 70.6～84.9% get the food area, obviously reduce this worm causing harm to the cabbage heart plant.And, after using the 5th day, embodiment 2～8 preparations still can play fine inhibition feeding effect to the phyllotreta striolata adult, can reduce this worm handle on the blade 75.2～83.4% get the food area.
Embodiment 10
Get the chloroform extract of 0.1g lantana methanolic extract, with 1ml methyl alcohol it dissolved, in chloroform extract solution, add 100ml water and 0.5ml Tween 80 in 70 ℃ of water-baths, mixing, preparation 8.Get the chloroform extract of 0.1g lantana methanolic extract, with 1ml methyl alcohol it dissolved, in chloroform extract solution, add 200ml water and 1ml tween degree 80 in 70 ℃ of water-baths, mixing, preparation 9.By the above-mentioned influence of shrouding method mensuration preparation 8 and 8 pairs of phyllotreta striolata imago feedings.
Experimental result shows, preparation 1 and 9 significantly suppresses the foods of getting of phyllotreta striolata adults, reduce respectively this worm handle on the blade 56.2% and 40.7% get the food area, obviously reduce this worm causing harm to the cabbage heart plant.And, after using the 6th day, preparation 8 and 9 still can play significant inhibition feeding effect to the phyllotreta striolata adult, can reduce respectively this worm handle on the blade 54.7% and 34.3% get the food area.
Claims (7)
1. a phyllotreta striolata imago feeding inhibitor is characterized in that being made up of lantana extract solution, water and surfactant, and the volume ratio of described lantana extract solution, water and surfactant is 2: 100～400: 0.5～2; Described lantana extract solution be with the methanolic extract of 1 weight portion lantana with the methyl alcohol of 5～10 weight portions in water-bath fully dissolving prepare; Or with total lantadene of 1 weight portion with 9～15 weight portion volume ratios be methyl alcohol-chloroform mixed liquor of 3: 1 in water-bath fully dissolving prepare.
2. phyllotreta striolata imago feeding inhibitor according to claim 1 is characterized in that described bath temperature is 60～70 ℃.
3. phyllotreta striolata imago feeding inhibitor according to claim 1, the methanolic extract that it is characterized in that described lantana is that the lantana blade is dried to constant weight at 50～70 ℃, is ground into dry powder, soaks dry powder with methyl alcohol at normal temperatures, filter, get methanol extract liquid; It is plain to golden yellow that methanol extract liquid is discolored with activated carbon, and filtration is removed methyl alcohol and prepared.
4. phyllotreta striolata imago feeding inhibitor according to claim 1, the chloroform extract that it is characterized in that described lantana are to adopt the methanolic extract of lantana to soak with chloroform, filter, and remove chloroform and prepare.
5. phyllotreta striolata imago feeding inhibitor according to claim 1, it is characterized in that described total lantadene is to adopt an amount of methyl alcohol 60～70 ℃ chloroform extract to be dissolved fully in water-bath, in leaving standstill below 0 ℃ 24 hours, treat that after crystal is separated out crystal being filtered nature dries the yellow powder that obtains.
6. phyllotreta striolata imago feeding inhibitor according to claim 1 is characterized in that described surfactant is a Tween 80.
7. the preparation method of the described phyllotreta striolata imago feeding of claim 1 inhibitor is characterized in that water and surfactant are added the lantana extract solution, and mixing promptly.
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| CN2008100261951A CN101223889B (en) | 2008-01-31 | 2008-01-31 | Food-intake preventer and preparing method thereof for striped flea beetle |
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| CN2008100261951A CN101223889B (en) | 2008-01-31 | 2008-01-31 | Food-intake preventer and preparing method thereof for striped flea beetle |
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| CN101223889B true CN101223889B (en) | 2011-07-27 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101785483B (en) * | 2009-12-29 | 2013-06-19 | 华南农业大学 | Phyllotreta striolata feeding deterrent and preparation method thereof |
| CN104273127B (en) * | 2014-10-25 | 2016-05-18 | 佛山市盈辉作物科学有限公司 | A kind of pesticides composition for killing nematodes containing lantadene first element and amino-oligosaccharide |
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Non-Patent Citations (4)
| Title |
|---|
| 董易之等.马缨丹总岩茨烯对小菜蛾和斜纹夜蛾幼虫的拒食作用.应用生态学报16 12.2005,16(12),2361-2364. |
| 董易之等.马缨丹总岩茨烯对小菜蛾和斜纹夜蛾幼虫的拒食作用.应用生态学报16 12.2005,16(12),2361-2364. * |
| 颜振敏等，42（6），.马缨丹提取物对黄曲条跳甲成虫的生物活性.昆虫知识42 6.2005,42(6),664-669. |
| 颜振敏等,42(6),.马缨丹提取物对黄曲条跳甲成虫的生物活性.昆虫知识42 6.2005,42(6),664-669. * |
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Effective date of registration: 20160411 Address after: 510530 Guangdong province high tech Industrial Development Zone of Guangzhou city science and technology innovation base on Road No. 80 D District 607-612 unit Patentee after: GUANGZHOU RUIFENG BIOTECHNOLOGY CO., LTD. Address before: 510642 Guangdong city of Guangzhou province Tianhe District Wushan Patentee before: South China Agricultural University |
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Inventor after: Li Shenlei Inventor after: Zhang Maoxin Inventor after: Dong Yizhi Inventor after: Ling Bing Inventor after: Liang Guangwen Inventor after: Song Yanxia Inventor after: Lin Xiaojun Inventor after: Chen Fang Inventor after: Jin Xin Inventor before: Zhang Maoxin Inventor before: Dong Yizhi Inventor before: Ling Bing Inventor before: Liang Guangwen Inventor before: Song Yanxia |
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