CN106018045A - Pipetting mechanism for sectioning and staining cells - Google Patents

Pipetting mechanism for sectioning and staining cells Download PDF

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Publication number
CN106018045A
CN106018045A CN201610518685.8A CN201610518685A CN106018045A CN 106018045 A CN106018045 A CN 106018045A CN 201610518685 A CN201610518685 A CN 201610518685A CN 106018045 A CN106018045 A CN 106018045A
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China
Prior art keywords
assembly
liquid
suction pipe
inner suction
pipetting device
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Pending
Application number
CN201610518685.8A
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Chinese (zh)
Inventor
邱加伟
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Individual
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Individual
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Priority to CN201610518685.8A priority Critical patent/CN106018045A/en
Publication of CN106018045A publication Critical patent/CN106018045A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

Abstract

The invention relates to a pipetting mechanism for sectioning and staining cells. The pipetting mechanism comprises a vertical rotating shaft, a transverse rotating arm and a pipetting assembly, wherein the transverse rotating arm is arranged at the upper end part of the rotating shaft, and the pipetting assembly is arranged at the outer side end of the rotating arm; the pipetting assembly comprises an inner suction tube which is vertically arranged and a liquid suction and discharging component and a washing component A which are separately connected with an upper tube opening of the inner suction tube. The object of the invention is to provide the pipetting mechanism for sectioning and staining the cells. The pipetting mechanism has the advantages that the pipetting mechanism is diversified in function and can be used for completing operations such as sampling, liquid adding, washing and waste liquid suction, so that the manufacturing cost is reduced; the pipetting mechanism is provided with the washing component A, thus the internal tube wall of the inner suction tube can be washed, and an outer sleeve further sleeves the inner suction tube and can be used for washing an outer tube wall of the inner suction tube in a manner of being matched with a washing component B, thereby achieving the effect of preventing pollution.

Description

Pipetting device for sectioning cells dyeing
Technical field
The present invention relates to a kind of pipetting device for sectioning cells dyeing.
Background technology
In medical science detection and biological study, often need by cell sample is carried out film-making and dyeing, with The most preferably observe.Traditional film-making dyeing course all uses manual operations, but owing to manual smear exists The interference factor such as substantial amounts of cell accumulation and erythrocyte, mucus, impurity and affect correct diagnosis, can go out The false negative rate of existing 2%~50%, and easily make diagnostic personnel eye work fatigue, and waste time and energy, effect The problems such as rate is low.In recent years, the application that liquid basal cell learns a skill makes the film-making of cell and staining technique have newly Breakthrough, it is a kind of to be saved in cell-preservation liquid by exfoliative cyte that liquid basal cell learns a skill, and can effectively dispel Cell mass dispersion is also dissociated by the interference diagnosis factor such as erythrocyte, mucus, impurity, significantly changes pathological cells Length of schooling tablet quality, cellularity and clear background, beneficially Differential Diagnosis, hence it is evident that reduce false negative rate.
Summary of the invention
It is an object of the invention to provide a kind of pipetting device for sectioning cells dyeing.
The purpose of the present invention is achieved through the following technical solutions: a kind of pipetting device for sectioning cells dyeing, It includes vertical rotating axle, the lateral rotation arm being located at rotary shaft upper end and is located at cursor outboard end Move liquid assembly;Described shifting liquid assembly includes vertically arranged inner suction pipe and is connected with inner suction pipe upper orifice respectively Suction tapping assembly and cleaning assembly A;Described suction tapping assembly is used for the sample on pipette samples hole and by its turn Move to fill on liquid film-making assembly or for drawing the waste liquid on dress liquid film-making assembly;Described cleaning assembly A uses In the inner tubal wall cleaning inner suction pipe.
For prior art, it is an advantage of the current invention that:
1. pipetting device functional diversities, can complete sampling, liquid feeding, clean and draw the operations such as waste liquid, Save manufacturing cost.
2. pipetting device arranges cleaning assembly A, can be rinsed the inner tubal wall of inner suction pipe, and on inner suction pipe Also it is arranged with trocar sheath, matching purge assembly B, the outer tube wall of inner suction pipe can be rinsed, reach to prevent dirt The effect of dye.
3. cannelure top is provided with liquid storage cylinder, is once concentrated cleanout fluid by liquid storage cylinder, can make ring Shape groove full cleanout fluid suitable for reading, when cleanout fluid flows downwardly into cannelure, can be carried out inner suction pipe outer wall without dead The flushing at angle.
Accompanying drawing explanation
Fig. 1 is the perspective view of a kind of pipetting device embodiment for sectioning cells dyeing of the present invention.
Fig. 2 is the structural representation at another visual angle of pipetting device for sectioning cells dyeing.
Fig. 3 is the top view of the pipetting device for sectioning cells dyeing.
Fig. 4 is the A-A sectional view in Fig. 3.
Fig. 5 is zoomed-in view at A in Fig. 4.
Fig. 6 is the structural representation moving liquid assembly.
Fig. 7 is the structural representation of dyeing mechanism.
Label declaration: 1 frame, 11 framework platforms, 2 setting-out districts, 21 sample wells, 30 drive motors, Pipe, 36 cleaning groups are put in 31 rotary shafts, 32 cursors, 33 inner suction pipes, 34 suction tapping assemblies, 341 suctions Part A, 361 reserving liquid tanks, 362 cleaning fluid conveying pipelines, 363 check valves, 37 trocar sheaths, 371 cannelures, 372 liquid storage cylinders, 373 helical ducts, 38 cleaning assembly B, 39 constant speed modules, 4 producer agencies, 41 from Cartridge, 42 dress liquid film-making assemblies, 5 dyeing mechanisms, 51 fixed supports, 52 dyeing liquor conveying pipes, 53 dyes Color liquid case, 6 collection hole, 7 flow pumps.
Detailed description of the invention
Below in conjunction with Figure of description and embodiment, present invention is described in detail:
The enforcement of a kind of pipetting device for sectioning cells dyeing provided for the present invention as shown in Figures 1 to 6 Illustrate and be intended to.
A kind of pipetting device for sectioning cells dyeing, it includes vertical rotating axle 31, is located at rotary shaft 31 The lateral rotation arm 32 of upper end and be located at the shifting liquid assembly of cursor 32 outboard end;
Described shifting liquid assembly includes vertically arranged inner suction pipe 33 and is connected with inner suction pipe 33 upper orifice respectively Suction tapping assembly 34 and cleaning assembly A36;
Described suction tapping assembly 34 is used for the sample on pipette samples hole and transfers them to fill liquid film-making assembly Or for drawing the waste liquid on dress liquid film-making assembly;Described cleaning assembly A36 is for cleaning inner suction pipe 33 Inner tubal wall.
Described cleaning assembly A36 includes the reserving liquid tank 361 for store washing liquid, is connected to inner suction pipe 33 and Cleaning fluid conveying pipeline 362 between reserving liquid tank 361 and flow pumps, described cleaning fluid conveying pipeline 362 sets Put check valve 363.Check valve 363 is disposed to prevent inhaling tapping assembly 34 and draws waste liquid or sample liquid Time pollute the cleanout fluid in reserving liquid tank 361.Described cleanout fluid is distilled water.
Move liquid assembly when pipette samples or waste liquid, the inside and outside tube wall of inner suction pipe 33 all can residual contaminants, Therefore need the inside and outside tube wall of inner suction pipe 33 is cleaned simultaneously, can be only achieved the effect preventing polluting.
Described shifting liquid assembly also includes trocar sheath 37 and and the trocar sheath being set in inner suction pipe 33 outer wall top The 37 cleaning assembly B38 connected;
Trocar sheath 37 upper portion inner tube wall is tightly placed on inner suction pipe 33 outer wall, and trocar sheath 37 bottom inner tubal wall is with interior Leaving gap between suction pipe 33 outer wall and form cannelure 371, cannelure 371 top is with cleaning assembly B38 even Connect.
Described cannelure 371 top is provided with liquid storage cylinder 372, and described liquid storage cylinder 372 is in funnel-form.
When cleaning assembly B38 carries cleanout fluid in liquid storage cylinder 372, due to the feed liquor speed of liquid storage cylinder 372 Degree is more than liquid speed, and short time cleaning liquid inside is concentrated in liquid storage cylinder 372, when cleaning assembly B38 stops When only carrying, the cleanout fluid in liquid storage cylinder 372 can flow downwardly in cannelure 371, and to inner suction pipe 33 outside Wall is rinsed.
By liquid storage cylinder 372, cleanout fluid is once concentrated, cannelure 371 full cleaning suitable for reading can be made Liquid, when cleanout fluid flows downwardly into cannelure 371, can carry out the flushing without dead angle to inner suction pipe 33 outer wall.
The liquid storage cylinder 372 of funnel-shaped structure can increase cleanout fluid flushing pressure.
Cleaning assembly B38 is identical with the structure of cleaning assembly A36.
Axial screw passage 373 centered by inner suction pipe 33 is offered in described trocar sheath 37;Described spiral leads to Top, road 373 connection cleaning assembly B38, its underpart connection liquid storage cylinder 372.
Described suction tapping assembly 34 includes inhaling puts pipe 341 and the flow pumps of band function of measuring.Described inner suction pipe 33 upper orifices, pipe 341 is put in suction and cleaning fluid conveying pipeline 362 is connected by tee T.
Described rotary shaft 31 lower end is provided with driving motor 30, and described driving motor 30 is controlled by constant speed module 39 Oscilaltion processed.
When driving motor 30 to drive shifting liquid assembly to turn to directly over sample well 21 or dress liquid film-making assembly 42, Constant speed module 39 controls to move in liquid assembly drops to sample well 21 or dress liquid film-making assembly 42 and carries out imbibition or put Liquid, then constant speed module 39 controls to move liquid assembly and rises reset.
Described constant speed module 39 includes vertically arranged screw rod and the sliding shoe coordinated with screw flight, described Sliding shoe is fixing with driving motor 30 to be connected, and when screw rod rotates, sliding shoe drives and drives motor 30 to carry out Oscilaltion campaign.
Pipetting device for sectioning cells dyeing provided by the present invention is Full Automatic Liquid based thin-layer cell film-making A portion of dyeing apparatus.
Full Automatic Liquid based thin-layer cell slide-making staining machine includes frame 1, the setting-out being located on framework platform 11 District 2, the producer agency 4 being located at setting-out district 2 side and the dyeing mechanism 5 that producer agency 4 is dyeed;
Described producer agency 4 includes centrifugal pan 41 and some dress liquid set up separately on centrifugal pan 41 outer circumferential side Film-making assembly 42.Producer agency 4 sees earlier patent application: Thinprep pap test pelletizer, the patent No.: 201510422833.1。
Described setting-out district 2 includes some spaced sample wells 21, and described sample well 21 is with rotary shaft 31 Centered by annular spread;Border circular areas formed by described producer agency 4 intersects or tangent with setting-out district 2, Setting-out district 2 and producer agency 4 intersection are not provided with sample well 21, i.e. various kinds sample wells 21 interval arranges formation Arcuate structure centered by rotary shaft 31.
Described shifting liquid assembly just to various kinds sample wells 21 or dress liquid film-making assembly 42 directly over.
Move liquid assembly to be driven around setting-out district 2 center rotating by rotary shaft 31, turn to sample when moving liquid assembly Time directly over hole 21, can sample in pipette samples hole 21;
When moving liquid assembly and turning to setting-out district 2 with producer agency's 4 intersecting area, move liquid assembly just to dress liquid Directly over film-making assembly 42, the sample of absorption can be put to dress liquid film-making assembly 42 or draw dress liquid film-making Waste liquid in assembly 42.
In described setting-out district 2, at least a sample well 21 is the collection hole for collecting waste liquid or cleanout fluid 6.Collect hole 6 to be connected with waste liquid cylinder.
When moving liquid assembly and turning to collect directly over hole 6, cleanout fluid is also drained into receipts by shifting liquid assembly capable of washing Drain in collection hole 6 in collection hole 6 or by the waste liquid drawn in dress liquid film-making assembly 42.
Described dyeing mechanism 5 includes the fixed support 51 being located on framework platform 11 and some is respectively used to The dyeing liquor conveying assembly of conveying coloured differently liquid;
Each dyeing liquor conveying assembly includes the dyeing liquor case 53 for storing dyeing liquor, is connected to dyeing liquor case 53 On dyeing liquor conveying pipe 52 and flow pumps;The liquid outlet of each dyeing liquor conveying pipe 52 is located at fixed support On 51, and its lower nozzle just to same dress liquid film-making assembly 42 above.
The liquid outlet of dyeing liquor conveying pipe 52 is vertically arranged, and the liquid outlet tube wall of each dyeing liquor conveying pipe 52 Against forming drain pipe group.
In the present embodiment, it is provided with four groups of dyeing liquor conveying assemblies altogether according to the colouring method used, uses respectively In conveying ethanol, A liquid, B liquid and C liquid.Each dyeing liquor conveying pipe 52 is arranged on same fixed support On 51, not only simplify structure, save machine cost, and be easy to the programme-control of producer agency 4.
Work process:
One. sampling
Motor 30 is driven to drive inner suction pipe 33 to turn to directly over sample well 21, in constant speed module 39 controls Suction pipe 33 decline is stretched in sample well 21, inhales tapping assembly 34 and sample liquid is sucked in inner suction pipe 33;In Suction pipe 33 rises and turns to fill directly over liquid film-making assembly 42, and dress liquid film-making group is stretched in inner suction pipe 33 decline In part 42, inhale tapping assembly 34 and sample liquid is put into dress liquid film-making assembly 42;Inner suction pipe 33 rises also Turning to collect directly over hole 6, tube wall inside and outside inner suction pipe 33 is entered by cleaning assembly A36 and cleaning assembly B38 Row rinses, and cleanout fluid flows into be collected in hole 6;
Inner suction pipe 33 turns to directly over another sample well 21, and centrifugal pan 41 rotates a working position, repeats Above-mentioned sampling process.
Two. sample preparation
After having sampled, it is centrifugal that centrifugal pan 41 drives dress liquid film-making assembly 42 to rotate.
Three. dyeing
After centrifugal sample preparation completes, dress liquid film-making assembly 42 is parked in immediately below the drain pipe group of dyeing mechanism 5, dye Color liquid conveying assembly adds ethanol in dress liquid film-making assembly 42, and centrifugal pan 41 rotates a working position, weight Multiple above-mentioned ethanol adds operation.
After having added all of dress liquid film-making assembly 42, centrifugal pan 41 drives dress liquid film-making assembly 42 to rotate Centrifugal.
After being centrifuged, motor 30 is driven to drive inner suction pipe 33 to turn to fill directly over liquid film-making assembly 42, Constant speed module 39 controls inner suction pipe 33 decline and stretches in dress liquid film-making assembly 42, and inhaling tapping assembly 34 will dress Waste liquid in liquid film-making assembly 42 sucks in inner suction pipe 33, and inner suction pipe 33 rises and turns to just collecting hole 6 Top, inhales tapping assembly 34 and is entered in collection hole 6 by the waste liquid in dress liquid film-making assembly 42, and by clear Wash assembly A36 and cleaning assembly B38 tube wall inside and outside inner suction pipe 33 is rinsed.
After flushing completes, inner suction pipe 33 adds cleanout fluid to dress liquid film-making assembly 42 successively, and centrifugal pan 41 carries Dynamic dress liquid film-making assembly 42 rotates centrifugal;After being centrifuged, extract waste liquid by inner suction pipe 33;Dyeing liquor Conveying assembly adds A liquid to dress liquid film-making assembly 42, extracts waste liquid after being centrifuged;Inner suction pipe 33 is to dress liquid system Chip module 42 adds cleanout fluid, extracts waste liquid after being centrifuged;Dyeing liquor conveying assembly is to dress liquid film-making assembly 42 Add ethanol, after being centrifuged, extract waste liquid;Dyeing liquor conveying assembly adds B liquid to dress liquid film-making assembly 42, from Waste liquid is extracted after the heart;Inner suction pipe 33 adds cleanout fluid to dress liquid film-making assembly 42, extracts waste liquid after being centrifuged; Dyeing liquor conveying assembly adds C liquid to dress liquid film-making assembly 42, completes dying operation.

Claims (7)

1. the pipetting device for sectioning cells dyeing, it is characterised in that: it includes vertical rotating axle (31), it is located at the lateral rotation arm (32) of rotary shaft (31) upper end and is located at cursor (32) outward The shifting liquid assembly of side;
Described shifting liquid assembly includes vertically arranged inner suction pipe (33) and above manages with inner suction pipe (33) respectively Suction tapping assembly (34) of mouth connection and cleaning assembly A (36);
Described suction tapping assembly (34) is used for the sample on pipette samples hole and transfers them to fill liquid film-making group On part or for drawing the waste liquid on dress liquid film-making assembly;Described cleaning assembly A (36) is used for cleaning interior suction The inner tubal wall of pipe (33).
Pipetting device for sectioning cells dyeing the most according to claim 1, it is characterised in that: institute State cleaning assembly A (36) to include the reserving liquid tank (361) for store washing liquid, be connected to inner suction pipe (33) And the cleaning fluid conveying pipeline (362) between reserving liquid tank (361) and flow pumps, described cleaning fluid conveying pipeline (362) check valve (363) is set on.
Pipetting device for sectioning cells dyeing the most according to claim 1, it is characterised in that: institute State shifting liquid assembly and also include being set in trocar sheath (37) and and the trocar sheath on inner suction pipe (33) outer wall top (37) the cleaning assembly B (38) connected;
Trocar sheath (37) upper portion inner tube wall is tightly placed on inner suction pipe (33) outer wall, trocar sheath (37) bottom Leave gap between inner tubal wall and inner suction pipe (33) outer wall and form cannelure (371), on cannelure (371) Portion is connected with cleaning assembly B (38).
Pipetting device for sectioning cells dyeing the most according to claim 3, it is characterised in that: institute Stating cannelure (371) top and be provided with liquid storage cylinder (372), described liquid storage cylinder (372) is in funnel-form.
Pipetting device for sectioning cells dyeing the most according to claim 4, it is characterised in that: institute Axial screw passage (373) centered by inner suction pipe (33) is offered in stating trocar sheath (37);Described spiral shell Rotation passage (373) top connection cleaning assembly B (38), its underpart connection liquid storage cylinder (372).
Pipetting device for sectioning cells dyeing the most according to claim 1, it is characterised in that: institute State suction tapping assembly (34) and include that pipe (341) and the flow pumps of band function of measuring are put in suction.
7., according to the pipetting device for sectioning cells dyeing described in claim 1-6 any one, it is special Levy and be: described rotary shaft (31) lower end is provided with driving motor (30), and described driving motor (30) is passed through Constant speed module (39) controls oscilaltion.
CN201610518685.8A 2016-07-04 2016-07-04 Pipetting mechanism for sectioning and staining cells Pending CN106018045A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610518685.8A CN106018045A (en) 2016-07-04 2016-07-04 Pipetting mechanism for sectioning and staining cells

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Application Number Priority Date Filing Date Title
CN201610518685.8A CN106018045A (en) 2016-07-04 2016-07-04 Pipetting mechanism for sectioning and staining cells

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107340172A (en) * 2017-08-14 2017-11-10 中生(苏州)医疗科技有限公司 A kind of integrated sample preprocessing system
CN107340171A (en) * 2017-08-14 2017-11-10 中生(苏州)医疗科技有限公司 A kind of separate type system for pretreating sample
CN107643201A (en) * 2017-10-31 2018-01-30 麦克奥迪(厦门)医疗诊断系统有限公司 A kind of membrane type cell monolayer pelleter and its flaking method
RU2763339C2 (en) * 2017-10-06 2021-12-28 Диагдев Device and method for coloring organic material on micro-preparation
CN107643201B (en) * 2017-10-31 2024-04-19 麦克奥迪(厦门)医疗诊断系统有限公司 Film type monolayer cell pelleter and pelleter method thereof

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CN203908840U (en) * 2014-06-09 2014-10-29 马艳华 Microcomputer-controlled liquid-based cell sheet preparation dyeing system
CN204261986U (en) * 2014-12-04 2015-04-15 爱威科技股份有限公司 A kind of sampling probe cleaning device for outer walls
CN105067397A (en) * 2015-07-17 2015-11-18 邱加伟 Liquid-based thin cell slide preparation device
CN105562389A (en) * 2014-10-16 2016-05-11 北京普利生仪器有限公司 Sampling needle cleaning system and sampling needle cleaning method
CN205749076U (en) * 2016-07-04 2016-11-30 邱加伟 Pipetting device for sectioning cells dyeing

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Publication number Priority date Publication date Assignee Title
US20020185161A1 (en) * 1996-03-18 2002-12-12 Roche Diagnostics Gmbh Device for cleaning pipette probes or stirrers
EP0825444A2 (en) * 1996-08-21 1998-02-25 JEOL Ltd. Washing device for automatic biochemical analyzer
CN202002835U (en) * 2010-09-13 2011-10-05 南京卡博生物科技有限公司 Centrifugal smear liquid-based cell sheet processing dyeing machine
CN203908840U (en) * 2014-06-09 2014-10-29 马艳华 Microcomputer-controlled liquid-based cell sheet preparation dyeing system
CN105562389A (en) * 2014-10-16 2016-05-11 北京普利生仪器有限公司 Sampling needle cleaning system and sampling needle cleaning method
CN204261986U (en) * 2014-12-04 2015-04-15 爱威科技股份有限公司 A kind of sampling probe cleaning device for outer walls
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CN205749076U (en) * 2016-07-04 2016-11-30 邱加伟 Pipetting device for sectioning cells dyeing

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107340172A (en) * 2017-08-14 2017-11-10 中生(苏州)医疗科技有限公司 A kind of integrated sample preprocessing system
CN107340171A (en) * 2017-08-14 2017-11-10 中生(苏州)医疗科技有限公司 A kind of separate type system for pretreating sample
RU2763339C2 (en) * 2017-10-06 2021-12-28 Диагдев Device and method for coloring organic material on micro-preparation
CN107643201A (en) * 2017-10-31 2018-01-30 麦克奥迪(厦门)医疗诊断系统有限公司 A kind of membrane type cell monolayer pelleter and its flaking method
CN107643201B (en) * 2017-10-31 2024-04-19 麦克奥迪(厦门)医疗诊断系统有限公司 Film type monolayer cell pelleter and pelleter method thereof

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Application publication date: 20161012