CN105993288A - Treatment method for promoting germination of common andrographis herb seeds (including stored seeds) - Google Patents
Treatment method for promoting germination of common andrographis herb seeds (including stored seeds) Download PDFInfo
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- CN105993288A CN105993288A CN201610316914.8A CN201610316914A CN105993288A CN 105993288 A CN105993288 A CN 105993288A CN 201610316914 A CN201610316914 A CN 201610316914A CN 105993288 A CN105993288 A CN 105993288A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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Abstract
The invention disclose a treatment method for promoting germination of common andrographis herb seeds (including stored seeds), belongs to the technical field of plant seed germination and seedling growing and aims at solving the technical problems that the germination potential and germination rate of the common andrographis herb seeds, particularly the seeds stored at room temperature, are hard to increase. The method comprises the following main steps that 1, the seeds are cleaned; 2, gibberellin (GA3) and 6-benzyladenine (6-BA) solutions with different concentrations are used for soaking the seeds; 3, polyethylene glycol-6000 (PEG-6000) solutions with different concentrations are used for soaking the seeds; 4, flowing water is used for washing the seeds for a period of time; 5, alternatively, the steps 2, 3 and 4 are not carried out, the seeds in the step 1 are directly washed with flowing water for a period of time. The treatment method can be used for increasing the utilization rate of the common andrographis herb seeds, particularly the seeds stored at room temperature, simplifying the storage technology and lowering the storage cost.
Description
Technical field
The invention belongs to plant seed germination seedling-raising technique field, relate to a kind of processing method promoting Herba Andrographis seed (containing seed after storage) to germinate.
Background technology
Herba Andrographis (Andrographis paniculata (Burm.f.) Nees) is Acanthaceae Herba Andrographis platymiscium, originates in South Asia, large-scale use after China's introduction, has promoted it and entered American Pharmacopeia.The dry aerial parts of Herba Andrographis are made medicinal, and effective for multi-infection disease, market demand is bigger；Research finds, its extract andrographolide etc., except having antiinflammatory, protects the liver outside isoreactivity, has certain inhibitory action for HIV (human immunodeficiency virus) etc..
In the plantation of Herba Andrographis, the method using seminal propagation (spring sowing).Herba Andrographis seed is outer, and by waxiness, seed coat has certain thickness, there are some researches show, himself is containing the material of likely sprout inhibition.The quality of commercially available seed is irregular, and a domestic research shows, in its 22 parts of different seed samples collected, there is the germination percentage of 6 parts of seeds about 40%, the germination percentage of 13 parts of seeds is about 70%, and only the germination percentage of 3 parts of seeds can reach more than 80% (He Rui etc., 2011).Another research shows, the Herba Andrographis percentage of seedgermination after room temperature storage 2 years is 0%, it is necessary to 4 DEG C of cold preservations (Tong Jia etc., 2012).
The original producton location of Herba Andrographis and China domestic all carried out many about promote Herba Andrographis seed germination research (Li Ling prunus mume (sieb.) sieb.et zucc. and Li Ming, 2011；Tong Jia etc., 2011；Bhattacharya S, et al., 2012；Talei D, et al., 2012) use high temperature lamination, accelerated ageing and air plasma technology effective to improving germination percentage, but need calorstat, the special installation such as exsiccator and plasma generator.Use KNO3Or thiourea processes and can improve germination percentage, but agricultural KNO3Research can not also be had by alternate test reagent with thiourea.The method of conventionally employed friction seed coat, because the dynamics of friction, the degree galling seed coat is relatively difficult to hold, and the result difference of different tests is bigger.All experimental studies all require for the germination temperature of Herba Andrographis seed, generally 25 DEG C, temperature relatively low and higher time, germination percentage the most undesirable (Kumar B, et al., 2011), do not meet the feature that temperature Change in actual production is big.
Document is had to show (Rawat R and Vashistha DP, 2012), appropriate GA3Process can improve the germination percentage of Herba Andrographis seed, uses PEG-6000 (Pan Chunliu etc., 2013) to process the Herba Andrographis seed after room temperature storage 2 years so that it is germination percentage reaches 20.5%.Flowing water is used to rinse the effect being Simulated rainwater, because plant seed always germinates in rainy season.Having document (Wang Baolong etc., 2012) to show, flowing water rinses the germination rate that can improve Manchurian Ash Seed.And being used in combination of three, not yet have been reported that.This research proves GA3, PEG-6000 and flowing water rinse combination and process the germinating capacity that can significantly improve Herba Andrographis seed.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of processing method promoting Herba Andrographis seed (seed containing after storage) to germinate, aim to solve the problem that the problems such as (after storage) Herba Andrographis potentiality of seed is low, germination percentage is low, to improve germination percentage and the use value of (after storage) Herba Andrographis seed, reduce seed waste, simplify germination and storage technique and reduce cost, to overcome the deficiency of prior art problem.
Technical scheme comprises the following steps:
(1) by the Herba Andrographis seed (seed containing after storage) of pure maturation, 3 times are washed with clear water；
(2) gibberellins (GA containing variable concentrations it is placed in3) and the solution (the commercially available pure water of water) of 6-benzyladenine (6-BA) in (content is respectively 0mg/L, 50mg/L GA3, 200mg/L GA3, 50mg/L 6-BA, 200mg/L 6-BA, 50mg/L GA3+ 50mg/L 6-BA, 50mg/L GA3+ 200mg/L 6-BA, 200mg/L GA3+ 50mg/L 6-BA, 200mg/L GA3+ 200mg/L 6-BA), soaking at room temperature 6 hours；
(3) take out the seed that step (2) has been soaked, wash 3 times with clear water, be placed in the PEG-6000 solution that concentration is 4.3% or 6.8%, soaking at room temperature 24 hours；
(4) take out step (3) seed that soaked, wash 3 times with clear water, be placed under flowing water that (tap water, flow is that every 1000 seeds use 180ml/ hour；Water Sproading) to rinse 12 or 24 hours, process terminates；Germination ambient temperature is room temperature；
(5) (2nd) or is not performed, (3) and (4) step, the seed of step (1) is placed directly within flowing water that (tap water, flow is that every 1000 seeds use 180ml/ hour；Water Sproading) to rinse 12,24,36 or 48 hours, process terminates；Test germination environment mean temperature is 18 DEG C, 22 DEG C, 25 DEG C and 28 DEG C (average room temperature).
Preferably, process chamber room temperature (room temperature in normal sowing season-spring can meet requirement) between 19-30 DEG C.
Preferably, GA in described step (2)3The concentration of solution is 50mg/L, and in step (3), the concentration of PEG-6000 solution is 6.8%, and the time that in step (4), flowing water rinses is 24 hours.
Preferably, in step (5), germination environment mean temperature is 22 DEG C, 25 DEG C or 28 DEG C (different time, not chummery temperature, the room temperature in normal sowing season-spring can meet requirement).
Computing formula:
Total grain number × 100% of son is planted experimentally in seed grain number/confession that germinating energy (%)=process is sprouted after terminating on the 72nd hour
Total grain number × 100% of son is planted experimentally in seed grain number/confession that germination percentage (%)=process is sprouted after terminating on the 7th
Beneficial effects of the present invention:
The present invention uses GA3Solution, PEG-6000 solution and flowing water flushing process the Herba Andrographis seed that quality is the worst so that it is germinating energy reaches 41.9%, and germination percentage reaches 59.6%.Using flowing water flushing to process the new Herba Andrographis seed produced, germinating energy is up to 77.3%, and germination percentage is up to 94.1%.Flowing water flushing is used to process the room temperature storage Herba Andrographis seed of 2 years so that it is germinating energy is up to 34.3%, and document report only 0.5%；Germination percentage is up to 71.3%, and document reports only 20.5%.
The present invention does not use disinfectant, and use medicine easily buy, flow hydraulic energy recuperation, low to germination environment and storage environment temperature requirement, easily operation, low cost, after the present invention processes, Herba Andrographis seed has the feature neat, germination percentage is high of germinateing, and can improve seed or the utilization rate of the bad seed of quality after Herba Andrographis seed is especially preserved.
For further instruction beneficial effects of the present invention, 4 factor 3 horizontal quadrature tests (often 100 seeds of group, are repeated 3 times) shown in table 1 are carried out, it is thus achieved that different initiations process the germination index table of lower Herba Andrographis seed.As shown in Table 2,6-BA seriously inhibits the sprouting of seed, is got rid of application.Considering germinating energy the highest, germination percentage is high, and plumular axis is longer and Seedling is heavier as selection gist；Selecting flowing water to rinse 24 hours, PEG-6000 concentration is 6.8%, GA3Concentration is the combination of 50mg/L, i.e. No. 9 process.By contrast, its germination index is significantly better than other 8 group.
Table 1 Herba Andrographis germination orthogonal test
Table 2 orthogonal experiments analysis
Detailed description of the invention
In conjunction with following example, the present invention is further explained.
Embodiment 1
Herba Andrographis seed the worst for the quality bought is carried out initiation process, comprises the steps:
(1) the Herba Andrographis seed after cleaning, washes 3 times with clear water, removes silt etc.；
(2) 50mg/L GA it is placed in3In solution, room temperature (average 20 DEG C) is soaked 6 hours；
(3) taking out the seed soaked, wash 3 times with clear water, be placed in the PEG-6000 solution that concentration is 6.8%, room temperature (average 20 DEG C) is soaked 24 hours；
(4) take out the seed that soaked, wash 3 times with clear water, be placed under flowing water that (flow is every 1000 seeds use 180ml/ hour for tap water, average temperature 20 DEG C；Water Sproading) to rinse 24 hours, process terminates.Paper bed germinates (filter paper or absorbent paper, lower same), and light culture, between germination ambient temperature is 19-30 DEG C.
By the process of embodiment 2, the germinating energy of the Herba Andrographis seed that quality is the worst can reach 41.9%, and germination percentage can reach 59.6%, is significantly higher than and does not processes the 23.5% and 53.2% of group.
Embodiment 2
The Herba Andrographis seed newly produced is carried out initiation process, comprises the steps:
(1) by the Herba Andrographis seed of pure maturation, 3 times are washed with clear water；
(2) clean seed is placed directly within flowing water (flow is that every 1000 seeds use 180ml/ hour for tap water, average temperature 25 DEG C；Water Sproading) to rinse 48 hours, process terminates.Paper bed, natural light, germination environment mean temperature is 25 DEG C.
By the process of embodiment 1, the new germinating energy producing Herba Andrographis seed can reach 77.3% (comparison 50.0%), and germination percentage can reach 94.1% (comparison 89.2%), than document (Li Ling prunus mume (sieb.) sieb.et zucc. and Li Ming, 2011；Bhattacharya S, et al., 2012；Rawat R and Vashistha DP, 2012) improve, or reach to use the top level of (Tong Jia etc., 2012) in special installation document.
Embodiment 3
The Herba Andrographis seed of room temperature storage 2 years (valve bag using PE material preserves, and puts in locker, does not use desiccant or drying equipment) is carried out initiation process, comprises the steps:
(1) by the Herba Andrographis seed of pure maturation, 3 times are washed with clear water；
(2) clean seed is placed directly within flowing water (flow is that every 1000 seeds use 180ml/ hour for tap water, water temperature 19-30 DEG C；Water Sproading) to rinse 36 or 48 hours, process terminates.Paper bed, natural light, germination environment mean temperature 22-28 DEG C.
Process by embodiment 3, preserve the Herba Andrographis potentiality of seed of 2 years up to 16.7% (48 hours, average 22 DEG C) and 34.3% (36 hours, average 28 DEG C), germination percentage is up to 69.7% (48 hours, average 22 DEG C) and 71.3% (36 hours, average 28 DEG C), it is above germinating energy 0.5% and germination percentage 20.5% that document (Pan Chunliu etc., 2013) shows.
Claims (4)
1. the processing method promoting that Herba Andrographis seed (seed containing after storage) germinates, it is characterised in that the method comprises the following steps:
(1) by the Herba Andrographis seed (seed containing after storage) of pure maturation, 3 times are washed with clear water；
(2) gibberellins (GA containing variable concentrations it is placed in3) and the solution (the commercially available pure water of water) of 6-benzyladenine (6-BA) in (content is respectively 0mg/L, 50mg/L GA3, 200mg/L GA3, 50mg/L 6-BA, 200mg/L 6-BA, 50mg/L GA3+ 50mg/L 6-BA, 50mg/L GA3+ 200mg/L 6-BA, 200mg/L GA3+ 50mg/L 6-BA, 200mg/L GA3+ 200mg/L 6-BA), soaking at room temperature 6 hours；
(3) take out the seed soaked in step (2), wash 3 times with clear water, be placed in the PEG-6000 solution that concentration (mass/volume) is 4.3% or 6.8%, soaking at room temperature 24 hours；
(4) take out the seed soaked in step (3), wash 3 times with clear water, be placed under flowing water that (tap water, water temperature is between 15-30 DEG C, and flow is every 1000 seeds use 180ml/ hour；Water Sproading) to rinse 12 or 24 hours, process terminates；
(5) (2nd) or is not performed, and (4) step (3), the seed of step (1) is placed directly within flowing water that (tap water, water temperature is between 15-30 DEG C, and flow is that every 1000 seeds use 180ml/ hour；Water Sproading) to rinse 12,24,36 or 48 hours, process terminates.
2. a kind of processing method promoting Herba Andrographis seed (seed containing after storage) to germinate as described in right 1 requires, it is characterised in that do not use any disinfectant, GA in step (2) in described step (1)3The concentration of solution is 50mg/L, and in step (3), the concentration of PEG-6000 solution is 6.8%.
3. a kind of processing method promoting Herba Andrographis seed (seed containing after storage) to germinate as described in right 1 requires, it is characterized in that the time that in described step (4), flowing water rinses is 24 hours, the time that in step (5), flowing water rinses is 36 hours or 48 hours.
4. a kind of processing method promoting Herba Andrographis seed (seed containing after storage) to germinate as described in right 1 requires, its feature in seed treatment room room temperature between 19-30 DEG C, water temperature is between 15-30 DEG C from the beginning, germination ambient temperature (i.e. process chamber room temperature) between 19-30 DEG C after end, to illumination without particular/special requirement (natural irradiation or light culture).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116171861A (en) * | 2023-02-28 | 2023-05-30 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Rapid propagation method of kidney seeds of Caiton |
-
2016
- 2016-05-10 CN CN201610316914.8A patent/CN105993288A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| CHINNAPPAN ALAGESABOOPATHI: "Seed biology of Andrographis elongata T. And. (Acanthaceae) – a valuable endemic medicinal plant of India", 《INTERNATIONAL JOURNAL OF BIOSCIENCES》 * |
| 潘春柳等: "PEG处理对新鲜和老化穿心莲种子萌发及幼苗生长的影响", 《种子》 * |
| 裕载勋等: "《中草药栽培技术》", 31 March 1986, 中国青年出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116171861A (en) * | 2023-02-28 | 2023-05-30 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Rapid propagation method of kidney seeds of Caiton |
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